LOCUS       DAJTUM010000001       135977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTUM010000001 DAJTUM010000000
VERSION     DAJTUM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 135977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 135977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..135977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO471_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO471_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6241440.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO471_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO471_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6241441.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO471_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO471_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO471_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO471_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6241442.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO471_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO471_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6241443.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO471_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO471_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6241444.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO471_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO471_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6241445.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO471_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO471_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6241446.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO471_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO471_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6241447.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO471_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO471_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6241448.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO471_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO471_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6241449.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO471_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO471_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6241450.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO471_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO471_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6241451.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO471_000014"
     CDS             18619..18894
                     /locus_tag="OO471_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241452.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO471_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO471_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6241453.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO471_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO471_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6241454.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO471_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO471_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6241455.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO471_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO471_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6241456.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO471_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO471_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO471_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO471_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6241457.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO471_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OO471_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO471_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO471_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6241458.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO471_000023"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO471_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6241459.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO471_000024"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO471_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO471_000025"
     CDS             complement(30355..31602)
                     /locus_tag="OO471_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6241460.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO471_000026"
     CDS             complement(31624..31950)
                     /locus_tag="OO471_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6241461.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO471_000027"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO471_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6241462.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO471_000028"
     CDS             33338..34594
                     /locus_tag="OO471_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6241463.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO471_000029"
     CDS             34709..35035
                     /locus_tag="OO471_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6241464.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO471_000030"
     CDS             complement(35176..36414)
                     /locus_tag="OO471_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6241465.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO471_000031"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO471_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6241466.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO471_000032"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO471_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO471_000033"
     tRNA            complement(40322..40397)
                     /locus_tag="OO471_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            40618..40977
                     /locus_tag="OO471_000034"
     CDS             40618..40977
                     /locus_tag="OO471_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6241467.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40979..41371
                     /locus_tag="OO471_000035"
     CDS             40979..41371
                     /locus_tag="OO471_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6241468.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41423..42838)
                     /gene="gltX"
                     /locus_tag="OO471_000036"
     CDS             complement(41423..42838)
                     /gene="gltX"
                     /locus_tag="OO471_000036"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6241469.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43097..43172
                     /locus_tag="OO471_000037"
     tRNA            43097..43172
                     /locus_tag="OO471_000037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43130..43132,aa:Val,seq:tac)
     gene            43217..43292
                     /locus_tag="OO471_000038"
     tRNA            43217..43292
                     /locus_tag="OO471_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43250..43252,aa:Val,seq:tac)
     gene            43297..43372
                     /locus_tag="OO471_000039"
     tRNA            43297..43372
                     /locus_tag="OO471_000039"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43330..43332,aa:Lys,seq:ttt)
     gene            43403..43528
                     /locus_tag="OO471_000040"
     ncRNA           43403..43528
                     /ncRNA_class="other"
                     /locus_tag="OO471_000040"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43648..43989
                     /locus_tag="OO471_000041"
     CDS             43648..43989
                     /locus_tag="OO471_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6241470.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(43980..44906)
                     /locus_tag="OO471_000042"
     CDS             complement(43980..44906)
                     /locus_tag="OO471_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6241471.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            44996..45994
                     /locus_tag="OO471_000043"
     CDS             44996..45994
                     /locus_tag="OO471_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6241472.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(45991..46209)
                     /locus_tag="OO471_000044"
     CDS             complement(45991..46209)
                     /locus_tag="OO471_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6241473.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46211..48226)
                     /gene="ligA"
                     /locus_tag="OO471_000045"
     CDS             complement(46211..48226)
                     /gene="ligA"
                     /locus_tag="OO471_000045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6241474.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48297..49283)
                     /gene="zipA"
                     /locus_tag="OO471_000046"
     CDS             complement(48297..49283)
                     /gene="zipA"
                     /locus_tag="OO471_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6241475.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49513..50274
                     /gene="cysZ"
                     /locus_tag="OO471_000047"
     CDS             49513..50274
                     /gene="cysZ"
                     /locus_tag="OO471_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6241476.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50459..51430
                     /gene="cysK"
                     /locus_tag="OO471_000048"
     CDS             50459..51430
                     /gene="cysK"
                     /locus_tag="OO471_000048"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6241477.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51814..52071
                     /gene="ptsH"
                     /locus_tag="OO471_000049"
     CDS             51814..52071
                     /gene="ptsH"
                     /locus_tag="OO471_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6241478.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52116..53843
                     /gene="ptsI"
                     /locus_tag="OO471_000050"
     CDS             52116..53843
                     /gene="ptsI"
                     /locus_tag="OO471_000050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6241479.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53884..54393
                     /gene="crr"
                     /locus_tag="OO471_000051"
     CDS             53884..54393
                     /gene="crr"
                     /locus_tag="OO471_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6241480.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54435..55286)
                     /gene="pdxK"
                     /locus_tag="OO471_000052"
                     /gene_synonym="thiJ"
     CDS             complement(54435..55286)
                     /gene="pdxK"
                     /locus_tag="OO471_000052"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6241481.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55391..55765
                     /locus_tag="OO471_000053"
     CDS             55391..55765
                     /locus_tag="OO471_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6241482.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55798..56521
                     /locus_tag="OO471_000054"
                     /pseudo
     CDS             55798..56521
                     /locus_tag="OO471_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56725..57636)
                     /gene="cysM"
                     /locus_tag="OO471_000055"
     CDS             complement(56725..57636)
                     /gene="cysM"
                     /locus_tag="OO471_000055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6241483.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57770..58867)
                     /gene="cysA"
                     /locus_tag="OO471_000056"
     CDS             complement(57770..58867)
                     /gene="cysA"
                     /locus_tag="OO471_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6241484.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58857..59732)
                     /gene="cysW"
                     /locus_tag="OO471_000057"
     CDS             complement(58857..59732)
                     /gene="cysW"
                     /locus_tag="OO471_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6241485.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59732..60565)
                     /gene="cysT"
                     /locus_tag="OO471_000058"
     CDS             complement(59732..60565)
                     /gene="cysT"
                     /locus_tag="OO471_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6241486.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60565..61581)
                     /gene="cysP"
                     /locus_tag="OO471_000059"
     CDS             complement(60565..61581)
                     /gene="cysP"
                     /locus_tag="OO471_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6241487.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61885..62676)
                     /gene="ucpA"
                     /locus_tag="OO471_000060"
     CDS             complement(61885..62676)
                     /gene="ucpA"
                     /locus_tag="OO471_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6241488.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62863..63663)
                     /gene="murR"
                     /locus_tag="OO471_000061"
     CDS             complement(62863..63663)
                     /gene="murR"
                     /locus_tag="OO471_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6241489.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63827..64723
                     /gene="murQ"
                     /locus_tag="OO471_000062"
     CDS             63827..64723
                     /gene="murQ"
                     /locus_tag="OO471_000062"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6241490.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64727..66151
                     /gene="murP"
                     /locus_tag="OO471_000063"
     CDS             64727..66151
                     /gene="murP"
                     /locus_tag="OO471_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6241491.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66331..67230)
                     /gene="yfeX"
                     /locus_tag="OO471_000064"
     CDS             complement(66331..67230)
                     /gene="yfeX"
                     /locus_tag="OO471_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6241492.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67326..67901)
                     /locus_tag="OO471_000065"
     CDS             complement(67326..67901)
                     /locus_tag="OO471_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6241493.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67962..68411)
                     /locus_tag="OO471_000066"
     CDS             complement(67962..68411)
                     /locus_tag="OO471_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6241494.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68398..68823)
                     /locus_tag="OO471_000067"
     CDS             complement(68398..68823)
                     /locus_tag="OO471_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6241495.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69037..69906
                     /gene="amiA"
                     /locus_tag="OO471_000068"
     CDS             69037..69906
                     /gene="amiA"
                     /locus_tag="OO471_000068"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6241496.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69910..70809
                     /gene="hemF"
                     /locus_tag="OO471_000069"
     CDS             69910..70809
                     /gene="hemF"
                     /locus_tag="OO471_000069"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6241497.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70815..71867)
                     /gene="eutR"
                     /locus_tag="OO471_000070"
                     /pseudo
     CDS             complement(70815..71867)
                     /gene="eutR"
                     /locus_tag="OO471_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71913..72413)
                     /gene="eutK"
                     /locus_tag="OO471_000071"
     CDS             complement(71913..72413)
                     /gene="eutK"
                     /locus_tag="OO471_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6241498.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72426..73085)
                     /gene="eutL"
                     /locus_tag="OO471_000072"
     CDS             complement(72426..73085)
                     /gene="eutL"
                     /locus_tag="OO471_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6241499.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74267..75058
                     /locus_tag="OO471_000073"
     CDS             74267..75058
                     /locus_tag="OO471_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241500.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75778..76173
                     /locus_tag="OO471_000074"
     CDS             75778..76173
                     /locus_tag="OO471_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6241501.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76191..76454
                     /locus_tag="OO471_000075"
     CDS             76191..76454
                     /locus_tag="OO471_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241502.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76477..77019
                     /locus_tag="OO471_000076"
     CDS             76477..77019
                     /locus_tag="OO471_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241503.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77158..77445
                     /locus_tag="OO471_000077"
     CDS             77158..77445
                     /locus_tag="OO471_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241504.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77484..77690
                     /locus_tag="OO471_000078"
     CDS             77484..77690
                     /locus_tag="OO471_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241505.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78002..78487
                     /locus_tag="OO471_000079"
     CDS             78002..78487
                     /locus_tag="OO471_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6241506.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78468..78746
                     /locus_tag="OO471_000080"
     CDS             78468..78746
                     /locus_tag="OO471_000080"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241507.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78739..80199
                     /locus_tag="OO471_000081"
     CDS             78739..80199
                     /locus_tag="OO471_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6241508.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80233..81042
                     /gene="murI"
                     /locus_tag="OO471_000082"
     CDS             80233..81042
                     /gene="murI"
                     /locus_tag="OO471_000082"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6241509.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81161..81855)
                     /locus_tag="OO471_000083"
     CDS             complement(join(81161..81609,81609..81855))
                     /locus_tag="OO471_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6241510.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82374..83747)
                     /gene="eutA"
                     /locus_tag="OO471_000084"
     CDS             complement(82374..83747)
                     /gene="eutA"
                     /locus_tag="OO471_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6241511.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83722..84201)
                     /gene="eutP"
                     /locus_tag="OO471_000085"
     CDS             complement(83722..84201)
                     /gene="eutP"
                     /locus_tag="OO471_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6241512.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84214..84549)
                     /gene="eutS"
                     /locus_tag="OO471_000086"
     CDS             complement(84214..84549)
                     /gene="eutS"
                     /locus_tag="OO471_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6241513.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84842..86998)
                     /gene="maeB"
                     /locus_tag="OO471_000087"
                     /pseudo
     CDS             complement(84842..86998)
                     /gene="maeB"
                     /locus_tag="OO471_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
     gene            86995..87651
                     /locus_tag="OO471_000088"
                     /pseudo
     CDS             86995..87651
                     /locus_tag="OO471_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase A"
     gene            87671..89674
                     /gene="tkt"
                     /locus_tag="OO471_000089"
     CDS             87671..89674
                     /gene="tkt"
                     /locus_tag="OO471_000089"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6241514.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(89777..90820)
                     /locus_tag="OO471_000090"
     CDS             complement(89777..90820)
                     /locus_tag="OO471_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6241515.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(90946..91521)
                     /gene="nudK"
                     /locus_tag="OO471_000091"
     CDS             complement(90946..91521)
                     /gene="nudK"
                     /locus_tag="OO471_000091"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6241516.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(91589..93568)
                     /gene="aegA"
                     /locus_tag="OO471_000092"
     CDS             complement(91589..93568)
                     /gene="aegA"
                     /locus_tag="OO471_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6241517.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            93774..95491
                     /gene="narQ"
                     /locus_tag="OO471_000093"
                     /pseudo
     CDS             93774..95491
                     /gene="narQ"
                     /locus_tag="OO471_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            95693..98806
                     /gene="acrD"
                     /locus_tag="OO471_000094"
     CDS             95693..98806
                     /gene="acrD"
                     /locus_tag="OO471_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6241518.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(98905..98964)
                     /gene="ypfM"
                     /locus_tag="OO471_000095"
     CDS             complement(98905..98964)
                     /gene="ypfM"
                     /locus_tag="OO471_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6241519.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            99345..99701
                     /locus_tag="OO471_000096"
     CDS             99345..99701
                     /locus_tag="OO471_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6241520.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            99705..100832
                     /gene="dapE"
                     /locus_tag="OO471_000097"
     CDS             99705..100832
                     /gene="dapE"
                     /locus_tag="OO471_000097"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6241521.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            100860..101060
                     /locus_tag="OO471_000098"
     CDS             100860..101060
                     /locus_tag="OO471_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6241522.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101170..101868)
                     /gene="ypfH"
                     /locus_tag="OO471_000099"
     CDS             complement(101170..101868)
                     /gene="ypfH"
                     /locus_tag="OO471_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6241523.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(101942..103957)
                     /gene="tmcA"
                     /locus_tag="OO471_000100"
     CDS             complement(101942..103957)
                     /gene="tmcA"
                     /locus_tag="OO471_000100"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6241524.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(103972..104835)
                     /locus_tag="OO471_000101"
     CDS             complement(103972..104835)
                     /locus_tag="OO471_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6241525.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105003..105716)
                     /gene="purC"
                     /locus_tag="OO471_000102"
     CDS             complement(105003..105716)
                     /gene="purC"
                     /locus_tag="OO471_000102"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6241526.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(105929..106963)
                     /gene="bamC"
                     /locus_tag="OO471_000103"
     CDS             complement(105929..106963)
                     /gene="bamC"
                     /locus_tag="OO471_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6241527.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(106980..107858)
                     /gene="dapA"
                     /locus_tag="OO471_000104"
     CDS             complement(106980..107858)
                     /gene="dapA"
                     /locus_tag="OO471_000104"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6241528.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108004..108576
                     /locus_tag="OO471_000105"
     CDS             108004..108576
                     /locus_tag="OO471_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6241529.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            108576..109046
                     /gene="bcp"
                     /locus_tag="OO471_000106"
     CDS             108576..109046
                     /gene="bcp"
                     /locus_tag="OO471_000106"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6241530.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            109299..109916
                     /gene="hyfA"
                     /locus_tag="OO471_000107"
     CDS             109299..109916
                     /gene="hyfA"
                     /locus_tag="OO471_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6241531.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            109916..111933
                     /gene="hyfB"
                     /locus_tag="OO471_000108"
                     /pseudo
     CDS             109916..111933
                     /gene="hyfB"
                     /locus_tag="OO471_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            111944..112891
                     /gene="hyfC"
                     /locus_tag="OO471_000109"
     CDS             111944..112891
                     /gene="hyfC"
                     /locus_tag="OO471_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6241532.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            112908..114347
                     /gene="hyfD"
                     /locus_tag="OO471_000110"
     CDS             112908..114347
                     /gene="hyfD"
                     /locus_tag="OO471_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6241533.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            114359..115009
                     /gene="hyfE"
                     /locus_tag="OO471_000111"
     CDS             114359..115009
                     /gene="hyfE"
                     /locus_tag="OO471_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6241534.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115014..116606
                     /gene="hyfF"
                     /locus_tag="OO471_000112"
     CDS             115014..116606
                     /gene="hyfF"
                     /locus_tag="OO471_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6241535.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            116596..118310
                     /gene="hyfG"
                     /locus_tag="OO471_000113"
                     /pseudo
     CDS             116596..118310
                     /gene="hyfG"
                     /locus_tag="OO471_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            118320..118865
                     /gene="hyfH"
                     /locus_tag="OO471_000114"
     CDS             118320..118865
                     /gene="hyfH"
                     /locus_tag="OO471_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6241536.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            118862..119620
                     /gene="hyfI"
                     /locus_tag="OO471_000115"
     CDS             118862..119620
                     /gene="hyfI"
                     /locus_tag="OO471_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6241537.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            119613..120026
                     /gene="hyfJ"
                     /locus_tag="OO471_000116"
     CDS             119613..120026
                     /gene="hyfJ"
                     /locus_tag="OO471_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6241538.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120056..121885
                     /gene="hyfR"
                     /locus_tag="OO471_000117"
     CDS             120056..121885
                     /gene="hyfR"
                     /locus_tag="OO471_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6241539.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            121886..122068
                     /locus_tag="OO471_000118"
     CDS             121886..122068
                     /locus_tag="OO471_000118"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6241540.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122090..122962
                     /locus_tag="OO471_000119"
                     /pseudo
     CDS             122090..122962
                     /locus_tag="OO471_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123000..124061)
                     /locus_tag="OO471_000120"
     CDS             complement(123000..124061)
                     /locus_tag="OO471_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6241541.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            124274..125737
                     /gene="bepA"
                     /locus_tag="OO471_000121"
     CDS             124274..125737
                     /gene="bepA"
                     /locus_tag="OO471_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6241542.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            125758..126117
                     /gene="arsC"
                     /locus_tag="OO471_000122"
     CDS             125758..126117
                     /gene="arsC"
                     /locus_tag="OO471_000122"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6241543.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(126250..126996)
                     /gene="hda"
                     /locus_tag="OO471_000123"
     CDS             complement(126250..126996)
                     /gene="hda"
                     /locus_tag="OO471_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6241544.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127046..128335)
                     /gene="uraA"
                     /locus_tag="OO471_000124"
     CDS             complement(127046..128335)
                     /gene="uraA"
                     /locus_tag="OO471_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6241545.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(128421..129047)
                     /gene="upp"
                     /locus_tag="OO471_000125"
     CDS             complement(128421..129047)
                     /gene="upp"
                     /locus_tag="OO471_000125"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6241546.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            129371..130408
                     /gene="purM"
                     /locus_tag="OO471_000126"
     CDS             129371..130408
                     /gene="purM"
                     /locus_tag="OO471_000126"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6241547.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            130408..131046
                     /gene="purN"
                     /locus_tag="OO471_000127"
     CDS             130408..131046
                     /gene="purN"
                     /locus_tag="OO471_000127"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6241548.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131218..133284
                     /gene="ppk1"
                     /locus_tag="OO471_000128"
     CDS             131218..133284
                     /gene="ppk1"
                     /locus_tag="OO471_000128"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6241549.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            133289..134830
                     /gene="ppx"
                     /locus_tag="OO471_000129"
     CDS             133289..134830
                     /gene="ppx"
                     /locus_tag="OO471_000129"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6241550.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(134869..>135977)
                     /gene="pdeF"
                     /locus_tag="OO471_000130"
     CDS             complement(134869..>135977)
                     /gene="pdeF"
                     /locus_tag="OO471_000130"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6241551.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        33932 a        34991 c        33426 g        33628 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccat
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attaccaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaagcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccgtc acgtaaagct tgtcgagttg acgggcggca tcatatgaat
    40441 tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg
    40501 caaatgaaca attttggaaa accgctcgca ttcagtagtt attcatgtca cggtttcctg
    40561 taaagtggtg ttataaaatg aactactaat agacccacat acattcaggg aattgttatg
    40621 ttcaaggaga ggatgacacc agatgaactt gccagactga ccggctatag ccgccagacc
    40681 attaataaat gggtacgcaa ggaaggctgg acaacgtcac caaaacctgg cgtccagggt
    40741 ggcaaagccc gactggttca cgtcaatgaa caggttcgtg aatatattcg caatgctgaa
    40801 cgtccagaag gtcagggaga agcgcctgcc ctttctggtg atgcgccgct tgaagtattg
    40861 ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa aacagtttac atccttgctt
    40921 ctgcgggaag ggattatcgg attgttacaa cgcttaggga ttcgcgatag caaataatat
    40981 gaaaagatta cgcaataaaa tgaccaccga agaactggct gaatgcctcg gtgtggctaa
    41041 acaaactgtt aaccgttgga ccagagaaaa aggctggaaa acggaaaaat ttcccggcgt
    41101 gaaaggcggt cgtgccagac ttattctggt cgatacgcaa gtttgcgagt ttattcagaa
    41161 cacgccagcc ttccataaca caccaatgtt gatggaagct gaagagagaa ttgctgaata
    41221 cgctcctgga gcacgcgccc cggcgtatcg acaaatcatc aacgcgatag ataatatgac
    41281 agacattgag caggaaaaag tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct
    41341 cgcccgtctc gatattgacg aatcagcata aataaaaaac ggcaggatat tatctcctgc
    41401 cgtttatctt tttacacgct aattactgct gattttcgcg ttcagcaata aaatccagcg
    41461 ctttgttgat acgctcgata ctgcgggtct taccaattgc gtgaacggta acatccagtg
    41521 ctggagactg ccccgcaccg gttacggcga cacgcagcgg cataccaact ttacccatac
    41581 ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg aacgttttca gcggtccagt
    41641 cagtaatcgc ggccagtttg tcacgaacca cttccagcgg ctgacgcgct accggacgca
    41701 gatgtttttt cgccgcatcg gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc
    41761 tctgtgccat ctctttcagc gtcttgcagc gttcgcccag cagtttcacc agatcagcca
    41821 gctgcgggcc gttacgggta tcgatatttt cctgctcaat gtgccactgt aagtgagtag
    41881 caacatactc cggcggcagc gcgttaatgt agtgatggtt cagccacagc agcttgtcgg
    41941 tgttgaacgc actggcagat ttgctgacgg cattcaaagt gaagtatttg atcatctctt
    42001 cacgagtgaa gatttcctga tcgccgtggg accagcccag acgcaccaga tagttcagca
    42061 gtgcttctgg caaataaccg tcatcacgat actgcattac gctgactgcc ccgtgacgtt
    42121 tggacagttt tttaccgtca tcgccattga tcatagaaac gtgcgcgtaa accggcaccg
    42181 gcgctttcag ggccttaaga atgttgatct ggcgcggcgt gttgttgata tggtcttcgc
    42241 cacggataac gtgggtgatt tccatatccc agtcatcgac aaccacacag aagttatagg
    42301 ttggggaacc atcggtacgg cggataataa gatcgtccag ttcctggttg ctgaactcga
    42361 tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta
    42421 caacacacgg ttcatcatca gcatgatgct catggctgtg gcggcagcga ccgtcataac
    42481 gcggcttctc acctttcgcc atttgctctt cgcgcagcgc ttccaggcgc tctttagagc
    42541 aatagcattt ataagcagtg ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat
    42601 caaaacgttt ggtctggtag tacggacctt catcccactc caggctcagc cagttcatgc
    42661 catccataat ggcttcgata gcttccggcg tggaacgctc aagatcggtg tcttcaatac
    42721 gcagcacgaa ctcaccgccg tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg
    42781 cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa gcgagttttg attttcatga
    42841 aatggcctta cgtttagaaa gatgccgaca accggcaaat cctggaaaaa ttgagtgggc
    42901 gatattctat cactcccgcc tgattcctca atgtagttcg ggtttctacc ttacgcatta
    42961 ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc gatcatcttg cttaaattta
    43021 cgacgaacga acaatttctt tagaaaatgc gttgactcat tttgaactct ccctataatg
    43081 cgactccaca cagcgggggt gattagctca gctgggagag cacctccctt acaaggaggg
    43141 ggtcggcggt tcgatcccgt catcacccac caactacttt atgtagtctc cgccgtgtag
    43201 caagaaattg agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg
    43261 ggtcggcggt tcgatcccgt catcacccac cacttcgggt cgttagctca gttggtagag
    43321 cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caatgtaaaa
    43381 aagcgcccta aaggcgcttt tttgctatct gcgatactca aagattcgaa cctgcagcag
    43441 gtttgagttg agcgcagcga aacaacggag ccgctcgcgg cgacggcccg aagggcgagc
    43501 gaagcgagtc atcctgcacg acccaccaat gcaaaaaagc gccctaaagg cgcttttttg
    43561 ctatctgcga tttgtaagat tgatttttct tctcaagttc cttgttcttc taccgatatt
    43621 acctcgtaca gtgacgagga ctgaacaatg gccactatct cttcaacttc aattccttcc
    43681 atacaaacac agagtagtaa cagggcatca caaggtagcg atgttgcttc acaaatcgcg
    43741 cggataagtc agcaaatcat caaactcact caacaaatta aagagattgt agacactagc
    43801 ggcagtgctg aagacaagca aaaacaggcc gaattgattc aacagcaaat caccctactg
    43861 gaaacacaac tggcgcagtt gcagaaacag caggcagaaa aggcgcagga gaaagagcag
    43921 cgtttgtctc tgaatgtatc ccttctaaat cctgttgaaa aaacgactca tatcgatatc
    43981 tatatctgat acaacggatc cccttcccga ccagccatta gcgcattgcc ctggtctcgt
    44041 accacgtccc ataacgcttc agcggcggtg gaaagtgaac ggttcttcct gcgcaccagc
    44101 atcaactggc gttcaaccac cggcgtaatg cgcttaacca ccagtgggct accttcaggc
    44161 aatggtagcg ccagtgcagg aagaatactg atgccgatgc ctgcggctac catcgggaac
    44221 agcgtcgccg gatgtccaat ctcctgcaca atattcgcct gaataccatt gcgcgccagc
    44281 gctgcgtcaa tcagcggtcg gctgcctgac gcgtaatcct gcaacaccaa tttcgctccc
    44341 tgtaatgcct gccagggtac ataatcttcc acggccaaag cgctatcgcg atggcaaagc
    44401 agaaagaaag gctcggaaag aatcgcttca cattgcagat cgcccactgg gcctggatcg
    44461 atgacgatgc caaagtcgac atctccctgg cgaatacttt ccatcaccca ctgctgcgga
    44521 cgatcatgca ggacaaactg aatatctgga tagcggcgat ggctttcagc aatgcattgc
    44581 ggaataagat gcgcggaaat ggtctggctg gcagcgaccc gaacttttcc gctcagttgt
    44641 tgccccatac gcccggtatc gcgcaacgtg ctgttaagtt catccagcag tcgctcaaga
    44701 cgcaaagcca actgctgccc tgcatctgta agcaccactt cacgcgtggt tctgtccagc
    44761 aggcgaacac cagtatgatt ttccagctcc ttcacactgt gacttactgc cgactggctc
    44821 aagccgatac gctctcctgc acgactaaaa cttttctcct gcgctactgt gacgaaaacc
    44881 tttaattgtt ttaaagaata attcatctgt tttcttcatg aatggatgca ataaatcaat
    44941 tttatttctc aaactggaag aagcacaata gaaccatcga tcatctggag tctttatgaa
    45001 actttttcgt atcctcgatc ctttcacctt aaccctgatc acggtggtgt tgctggcctc
    45061 tttctttccg gccagaggcg atttcgtccc cttctttgaa aatctgacca ccgcagctat
    45121 tgccctgctg ttctttatgc acggcgcgaa gttgtcgcgt gaggcgatta ttgctggcgg
    45181 tggtcactgg cgactgcatt tgtgggtaat gtgcagcacc ttcgtgctgt ttccgattct
    45241 gggtgtactg tttgcctggt ggaaaccggt aaatgtcgac ccgatgctct actccggttt
    45301 tctctacttg tgcattctcc cggctaccgt gcagtctgca atcgccttca cgtcaatggc
    45361 gggcggtaac gtcgcggcgg cggtttgttc tgcgtcagca tccagcctgc tggggatttt
    45421 cctttcacca ttgctggttg gtctggtgat gaatgttcac ggtgcaaggg gcagccttga
    45481 gcaggtcggt aaaattatgc tgcaactgct gctgccgttt gtgttggggc atctttcccg
    45541 gccgtggatt ggtgactggg tgtcgcgcaa taaaaaatgg attgcgaaaa ctgaccagac
    45601 gtccattctg ttggtggttt atacagcgtt cagcgaagcc gtcgttaatg gtatctggca
    45661 taaagttggc tggggatcat tgctgtttat cgtggtggtc agctgcgttc ttctggctat
    45721 cgtgattgta gttaacgtct ttatggcacg ccgactgagc ttcaataagg cagatgaaat
    45781 tactatcgtc ttttgtggtt cgaaaaagag tctggcaaat ggcatcccga tggcaaacat
    45841 tctgttcccc acatcggtga tcggtatgat ggtgctgccc ctgatgattt tccatcagat
    45901 ccaattgatg gtctgtgcgg tgctggcgcg tcgatacaaa cgccagaccg aacagttaca
    45961 ggcgcagcag gaaagcagcg ccgataaagc ttaaagcgga cgcttcaggg gctggaccag
    46021 ttgcgtcagc ccctcggttt taatcagcag cgtgatttgc attagctcac cgagcttccc
    46081 tgccgggaac tcatctttgc gggcaaacca cagcagatac tcctccggca ggtcaattaa
    46141 gcgacgccct ttgtatttgc caaacggcat tatcgtattg gctatttcaa tcagctgctc
    46201 tttttccatc tcagctaccc agcaaacgca gcatttccgc ttcgtcgatg acttcaatgc
    46261 ccagttcctg cgccttcgcc agtttagatc ctgcagcttc acccgctatc accagatcgg
    46321 ttttcttcga cacgctgccc gcgactttcg cgcccagttc gaccagtcga gctttagcgt
    46381 catcacgcga catctggctt aagctgcccg taagcaccac ggttttacca gcaaacgggc
    46441 tgtcaatctc ttccgcgttg ataacgatcg gcgcaggcca gtgaacacct tccgccaaca
    46501 gctcgctgat gacattgcgg ttgctttctt cggcaaagaa gttgtgaacg tgggatgcaa
    46561 cgacaatgcc aacatcaggc accttttgca gctcttcaat cgaagcggct tccagcgctt
    46621 ccagcgtgcc gaaatatgcc gccagacctg ctgcggtggc ctcgccgact tcacggatgc
    46681 caagtgcata gaggaagcga gcaaaggtgg tttctttcgc tttttccagc gcgttaacca
    46741 cgttttgtgc caattttggc cccatacgct ccagtccggt cagtttgcct gcggtgagtt
    46801 tgaacagatc tgccggagta tggacatatt ctttttcaac cagctgatcg atgattttgt
    46861 cgcccattcc gtcaacatcc atcgcacggc gggaaacaaa gtgtttcagc gactctttac
    46921 gctgcgcacc gcaaatcagg ccaccggtac agcgggcaac cgcttcacct tccacacgct
    46981 caacgtcaga accacatacc ggacaatgcg tcgggaatac aacctcacgg gtatcttccg
    47041 ggcgttcaga aagcacgacg ttaaccacct gcgggatcac gtcgccagcg cggcgaatca
    47101 ccactttatc gccaatgcgt aaaccaagac gttcgatttc atccgcattg tgtaaggttg
    47161 cgttactcac cagcacgcca gcaacatgga caggttccag acgcgcaaca ggcgtaatcg
    47221 cgccagtacg cccaacctga aactcgacgt cacgcacaaa ggtcatctgc tcctgcgccg
    47281 ggaatttaaa cgcaaccgcc cagcgcgggg cacgcgcgac aaagccaagc tgctcctgct
    47341 gttccagtga gttgacctta atcaccacgc cgtcgatatc aaagcccagc gtcgggcggt
    47401 cttcttccac tttgtgatag aacgccagca cttcttccgc cgattcacaa agcgttaccc
    47461 gatcgctgac cggcaccccc cactttttaa attgcagtaa acggccaaga tgagtatccg
    47521 gcagctcgcc accttccaga acaccaacgc catagcagaa aaaagtgagc ggtcgcttcg
    47581 ctgtaatacg cggatcaagc tgacgcagtg aaccagctgc cgcattacgt gggttagcaa
    47641 aaactttccc gcccgtgcgt cgcgcatctt cgttaatctt ttcgaacccc gcctgcggca
    47701 ggaacacttc accacgcact tccagacgcg ccgggatatt ctctccgtgc agcttcagcg
    47761 gaatggcgcg aatagtacgc acattagacg tgatatcttc cccggtggtg ccatcgccac
    47821 gggtcgcggc actgactaaa acgccatttt catacagaat actgacggca aggccatcca
    47881 gcttcagctc acagcaccag gtgactttct cgttgttttt cagacggtcc tgcacacgtt
    47941 tgttgaaagc aagaaagctt tcttcatcaa aaacgttatc cagtgacagc attggtactt
    48001 catggcgtat ctggctgaaa gctgccagcg gcgcagcacc tacacgttgg gtaggcgaat
    48061 caggcgtaat cagttctgga tgtttggttt ccagctcgcg cagttcgcgc atcagcctgt
    48121 cgtattcagc gtcgggaatt tccggcgcat ccatcacatg ataaagatat tcatgatggc
    48181 aaagcgtcgt tcgcagttct gtcagttgtt gttcgattga ttccatatcg caccatcaat
    48241 gctaaaaacc cccgacaagc gggggttcga agaggagtta atttgcctta agtgtatcag
    48301 gcgttggcgt ctttgacttc gcggatgatg tcctggtact cgcgcaattt ctgcggagtc
    48361 atcatacggc gctgatcgtc aagcacgaca ccgcccactt catcggcaat atgctgcgca
    48421 gattgcagca tcagcttgaa gttctgcagc tcgtcaccgt aagacggtac ctgcataaag
    48481 atagtgacac ccggagtagt gaaatccttc atttcaggat caaaggttcc cggtttcacc
    48541 atattcgcca ggctgaataa cgccgggccg ctgccatccg ggctaagatg acgatggtaa
    48601 atattcatat cgccaaaaat gaagcccgct tgttgaatgc tgttaagaag cagttcaccg
    48661 tttagctcgc taccgtgatg cgccgcgacg ttcatgataa tcaccgcttc tttgcgcttc
    48721 ggtttatcca taactggagc cggttccgct acaggctcag gctgtggtgc cgctacgggt
    48781 tctgcaggct ggaaagcctg ttgtgccggt tgcggtgctg aatgcacagg ctgcggcgct
    48841 ggcgcgacct gtggcgaaac tggctgctgc aacggctgtt caggctgcgg ctgataggca
    48901 ggctgctgca ccggctgcgc tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
    48961 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggtaggta ctggtgttgc
    49021 ggcgacggac gagcagcctc atgctcctga gcgttagccg gggcatgatt cacgcggtga
    49081 acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
    49141 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
    49201 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
    49261 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
    49321 aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt
    49381 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
    49441 gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag
    49501 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
    49561 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
    49621 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacaca gctcgatgtc
    49681 tggatcccga ctttcatgag ttacgttccg gactggctac aatggctgag ttatctgttg
    49741 tggcctctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc cacgattgct
    49801 aactggattg ccgctccgtt taacggttta ttggctgaac aactggaagc acgattgact
    49861 ggcgctacac cgccagatac cgggattttc ggtatcatga aagatgtgcc gcgaatcatg
    49921 aaacgcgaat ggcaaaaatt tgtctggtat ctgccgcgcg caattgtatt actaattctt
    49981 tacttcatcc ctggtattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc
    50041 tggatgttag ccatccagta ttgcgattac cccttcgata accacaaagt gccgtttaaa
    50101 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
    50161 agcctgttta cgatgatccc gctgcttaat ctgttcatca tgcccgttgc cgtttgtggc
    50221 gcgacggcga tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg gtaacaatct
    50281 accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc
    50341 atcttgcatg tcattatttc ccttctgtat atagatatgc taaatcctta cttccgcata
    50401 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
    50461 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
    50521 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
    50581 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
    50641 aggcgttgaa ctggttgaac cgaccagcgg taataccggg attgcactgg cctatgtagc
    50701 tgccgctcgc ggttacaaac tcaccctgac catgccagaa accatgagta ttgaacgccg
    50761 caagctgctg aaagcgttag gtgcaaacct ggtgctgacg gaaggtgcta aaggcatgaa
    50821 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
    50881 gcaacaattc agcaatccgg caaaccctga aattcacgaa aaaaccaccg gtccggagat
    50941 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
    51001 gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt
    51061 cgccgttgag ccaaccgatt ctccagttat cgcccaggtg ctggcaggtg aagagattaa
    51121 acctggcccg cataaaattc agggtattgg cgctggtttt atcccggcta acctcgatct
    51181 caagctggtc gataaagtca ttggcatcac caatgaagaa gccatttcta ccgcccgtcg
    51241 tctaatggaa gaagaaggta ttcttgcagg tatctcttct ggagcagctg ttgccgcagc
    51301 gttgaaacta caagaagatg aaagctttac caacaagaat attgtggtta ttctaccatc
    51361 atcgggtgag cgttatttaa gcaccgcatt gtttgccgat ctcttcactg agaaagaatt
    51421 gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc acctttttag gtgctttttt
    51481 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
    51541 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
    51601 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
    51661 acgtcagcgg tcaacacccg ccagcaatgg actgtattgc gctcttcgtg cgtcgcgtct
    51721 gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc
    51781 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
    51841 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
    51901 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
    51961 ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac
    52021 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
    52081 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
    52141 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc attgaccgga
    52201 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
    52261 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
    52321 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
    52381 tcatagccct gattaaagat aaacacatga cagctgacgc agctgctcat gaagttatcg
    52441 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
    52501 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
    52561 tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacgccg tccgaaaccg
    52621 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
    52681 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
    52741 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
    52801 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
    52861 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg
    52921 tagaagtatg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
    52981 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgctgccca
    53041 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg
    53101 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    53161 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggat cgtaaagaga
    53221 tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa ttgcgcatta
    53281 tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaatct
    53341 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    53401 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    53461 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    53521 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    53581 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg
    53641 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    53701 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    53761 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    53821 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    53881 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaaaaa ggataccgga
    53941 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    54001 gtcgtttttg cggaaaaaat cgttggtgat ggtattgcta tcaaaccaac gggtaacaaa
    54061 atggtcgcgc ctgtagacgg taccattggt aaaatctttg aaaccaatca cgcgttctct
    54121 atcgaatctg atagcggcgt tgaactgttc gtccacttcg gtatcgacac cgttgaactg
    54181 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggcgatact
    54241 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    54301 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtaaccgtg
    54361 ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc
    54421 atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc gctctcatgc
    54481 tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg cacggtgcac cgcatcgttt
    54541 aacgccttcc ctttcagcaa accactgata agctgagcac aaaacaggtc gccagtccct
    54601 ttcaggtcgg tttttacccg tgaatgggaa atgacattca cgctgtcggc actgaccacc
    54661 accacctgca tctcctgatt ttcttcatta ccggaggcgc tggtaatcac cacccatttt
    54721 aatgtgtctg aaagcagact ttttgcggca gcaatggcac tgtcgagatc gcggcaattt
    54781 ttaccggtca ggatttccag ctcaaagata ttgggggtaa ttccctgcgc cagcggcagt
    54841 aaatattgtc gatacgcctc gggaaggtca ggtttgacat aaattccgct atcaatatcg
    54901 ccaatcaccg gatcgaccat gatcaatagg tcaggatggt ctttgcgtag cgcagtcagc
    54961 cactcggcaa ggattttgat ttgcgatgcc gttcccatat agcccgtggt tacagcacga
    55021 agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa ccattcgtcc
    55081 ggaatcgcac caccgtagaa agtgtcataa tgcggcgtat tgctcagcaa taccgtcggc
    55141 acggcaaaga cattcaagcc gttctgtttt atagcaggca ccgcaatgct gttgcctacg
    55201 cttccgtaca ctacctgcga ctgcacggcg acgatatccg cctgcagcgc cctactctta
    55261 tcgttaaaca acaacaaact actcatttaa ttttttctcc ttgccgatga tcctcatcgt
    55321 attccaaccg aaactttacc tgattctggc agtcaaatcg gctattacaa aacaaagata
    55381 aggtaattca gtgaagaaaa tcatttgtct ggccattacg ctgctaatga cactccccgc
    55441 ttacgcgaag ttaactgttc atgaagaagc ccgcatcaac gccatgctgg agggattagc
    55501 acagaaaaag gatttgatat ttgtgcgcaa cggtgatgaa catacctgcg atgaagcggt
    55561 ttcccacctg cgtctgaagc tcggcaatac ccgtaaccgc attgacactg ccgagcagtt
    55621 tattgataag gtagcttcgt cgtcatcgat taccgggaag ccgtatatcg tgaagatccc
    55681 cggtaagagc gatgagaacg cacagccttt tttacatgcg ttaattgcgc agacggataa
    55741 aacggtgcct gcggaaggaa attaatccgc tttggtaagg catttacagg aggtaacatg
    55801 aaaaaatgct ttatttatca cgatgaaaaa tcgaataaat tttggtggat agattacgaa
    55861 ggggatagtt tagctgtcaa ctatggcaag gtaggtagta ttggtaaatt ccatacaaaa
    55921 gaattcgata atgaagaaca gtgtctgaaa gaagccagta aattgattgc cgcaaaaatg
    55981 aaaaaaggat atcaagaaac tccagacttt aacttcatgg atctctacta ttttgatgat
    56041 gaagagattg ggttacatct taaaacgtca cacccaaact tccagtgcca ttttactgat
    56101 ccactttata tgtgttgctg ggatgaagaa tctccttttg gtagcgatga aggtgctgat
    56161 gctctaaacg aacttgaaaa tagcctccgt tctggactgt gctgatttcc ctcaaatgtt
    56221 aattgaaact atgtggggta tgaaatacat cgctatggac agtattcttg aagaggatgt
    56281 gcgtgcgcaa ttactagccg atgaaatgag ctctatccag agcaatatga ttacctacgc
    56341 gactgcattc ggtcagatca aagtcatggg taaaatctcc cataaactta aaaagatggg
    56401 actcaatgca ctagcgcgtc atcagcttac cgcaaaaatt cttcaatggg gtgacggtca
    56461 ggactcacca atacttcaaa aaatgattga tgaccttact gcttttccac acgaaaatta
    56521 aaaactgcat ttgtcggcag caacaactgc cgacaaatca gattattgac aaagtgcact
    56581 ttgtttatgc cggatgcggc gtaaacgcct gatccggcat aaaaatattg ctaattcaat
    56641 atattgcaga aacattgtag gcctgataag cggagcgcat caggcagttt tgcgtttgtc
    56701 atcagtctcc aatgctatta atccttaaat ccctgccccc tgactaaaat gctcttcccc
    56761 aaacactccg gtggagaggt agcgatcgcc acgatcgcag atgatcgcta ccaccaccgc
    56821 accagggtta gcttttgcca cccgcagtgc tccagcaacc gcgccgcctg agctgacgcc
    56881 acagaatatt ccttcccgca ccgccagttc gcgcatggtg ttttccgcat cgcgctgatg
    56941 aatatccagc acctcatcca ccagcgcagc gttgaaaatc cccggcagat attccgcagg
    57001 ccagcggcga atgcccggaa tactgctgcc ttcttccggt tgcaggccaa caatggtcac
    57061 cggtttggat tgttcgcgca taaagcatga aacgccggtg atagtgccgg tcgtccccat
    57121 gctggagaca aaatgagtga tgcgcccgcc ggtttgctgc cagatttccg gcccggtggt
    57181 ggtgtaatgc gcataagggt tatcgggatt attgaactga tcgagcagct ttccttcgcc
    57241 acgattcgcc atctccagcg ccagatcgcg cgcaccttcc atgccctgct ctttggtgac
    57301 aagaatcagt tccgcaccat aagcacgcat cgccgcacgg cgttcctggc tcatgttgtc
    57361 gggcatcagc aatttcatgc gatagccttt cagcgcggca atcattgcca gcgcaatgcc
    57421 ggtgttacca ctggtggctt cgattaagac atcacccggt ttaatttccc cgcgcttttc
    57481 cgcctcgacg atcatcgaaa gtgccgcacg atctttcacc gaacctgccg ggttattgcc
    57541 ttccagtttt aaccacactt cactgccgtt atccggcccc attcgctgca acttcaccag
    57601 aggcgtattg cctattgttt gttctaatgt actcacaatc tctatccata cgtggtgttg
    57661 cctgatgcga cgcttgcgcg tcttatcagg cctacaggtt acaaaccttg ccattaaaaa
    57721 gcccggatcg cgggaagcgc ctccgggcgt ttaacattca ctcaacctat caggcgcttt
    57781 gtgcgagagc aagttcctca tcgcgggttt caatacgctc gtcgccgtta tacagccgcg
    57841 catgttgcag accaacgaat aaacgctcgc catgctgcgg ggcatcgtcg ccatgcatca
    57901 cgaccgtcag cggttcgttg taccacccca gcggctgcac cactaattgg gtgtagtgac
    57961 ctttcgggct ggcttccagt acctgtaccg gcagcggcga atcgagactg gtacggcggc
    58021 tgatatccac ttcccacggg cgcaggaaga gatccaccgg cccctgatac gcaggtgtgt
    58081 agcccagcgg ccagcgatgc gcgccaacat ggaactgccc gccgcgaatg gttccctgca
    58141 ggcggttcac ttcgcccata aattcgagta caaaacgggt cgctggttcg cgccatacct
    58201 gattcggcgc atcagcctgt tcaatattcc cctgactcat caccactaca cgatcggcta
    58261 cttcggtcgc ctcttcctgg tcgtgggtca caaacacgct ggtgaatttc agctcttcat
    58321 gcagttgacg cagccagcga cgcagctctt tacgcacctg cgcatccagc gcgccgaacg
    58381 gttcatcaag cagcagaatt tgcggttcta cagccagcgc gcgcgccagc gccacacgct
    58441 gtttctggcc gccggaaagc tgcgccggat aacgatccgc cagatgcgca agctggacca
    58501 tttccagcaa ttttgtcact ttcgctttga tggctgcggc attcgggcgc tcgcgacgcg
    58561 gcagcaccgt caggccaaaa gtgatattgt cgaataccgt catatggcgg aacagcgcgt
    58621 aatgctggaa cacgaaaccg actttacgat cgcgcgcgtg caggcgactc acgtcggtgc
    58681 cgtggaagcg aatatgcccg ctggtttgat gctccagccc ggcgataatc cgcagcagcg
    58741 tggttttccc ggaaccggac ggccccagca acgcgaccat ctgaccagaa ggaatatcca
    58801 gtgagatatc gttcagcacc tgggtgcgac caaaagactt cttgatattg gcaatctcaa
    58861 tgctcatgat gctcctcctg ctgcgcgcgt ttttcctgat tctccaggcg ccactgcaac
    58921 atactcttta aaaacagggt gataatcgcc atcaacgtca acagcgcggc ggcggtaaag
    58981 gagccgacgg tgttgtagtc ctgctccaac aattcaatct gtaacggcag cgaaagtgtt
    59041 tcgccgcgaa tcgagccgga aaccaccgac actgcaccaa actcgccaat cgcgcgggcg
    59101 ttggtcagca ccacgccgta aagcagcgcc cagcgaatat tcggcaatgt gacgcgacgg
    59161 aacatctgcc agccggacgc gccaagcaaa atcgccgctt cgtcttcctg gctgccctgg
    59221 cttaacatca ccggcaccag ctcgcgcacc acaaacggac acgtcacaaa gatggtgacc
    59281 agcaccattc cgggccagga gaacataatt tgcagattat gttcatccag ccagccgccg
    59341 agcgggccgt tggagccgta gaacagcaga tacaccagac cggcaaccac cggcgataca
    59401 gcaaacggaa tgtccagtag cgtcagcagt aactggcgtc ccgggaagtt aaagcgcgtc
    59461 accagccagg ccagcagaat gccgaacacc aggtttaccg gtacggcaat cagcgcgatc
    59521 atcaccgtca gccagatggc gtgcagcatg tccggatcgg ccagattctg taaaaccggc
    59581 atcagcccct tgctgaatgc ctgcacgaag atgtaaatca tcggcaccag caggatgaac
    59641 gccgaaacca gcatcccgat gccaatcaga aaccatttgc cccagttaat cgggcgcgcg
    59701 tcataacgct tcaattgggt aacttccgcc attaatgacc taccacacgc cgaccaaagc
    59761 gactttgcag agtgttaatt gagaacagca gcagcagaga tgccgcgagg atcaccgaag
    59821 caatcgcgct cgctgccggg taatcaaact cctgtaagcg cacaaaaatc atcagcgacg
    59881 tcacttccgt cttccacgcg atatttccgg cgataaaaat caccgcgcca aattcaccaa
    59941 gactacgggt aaacgacagc gccacgcccg ccaccagcgc cggagaaagc tccggcagca
    60001 ccactttgca gaaactctgc cagcgcgttg caccaagcgt ttccgccgct tcttcatatt
    60061 ccgggcctaa ctcttccagc accggctgca cggtacgcac cacaaacgga atgctggtaa
    60121 aggccatcgc caccgcaata ccaagccagg tataggtgac tttgatatca aacttcgcca
    60181 gccattcacc gtaaaaaccg tttacggaaa agagcgaggc cagcgttaaa ccggcgactg
    60241 ccgttggcag cgcaaagggt aaatccatca gcgcatcaag cagcgtgcgg cccggaaagc
    60301 gataacgtgt taaaatccac gccatcagca gaccgaaaac gccgttaaaa atcgatgcca
    60361 caaacgccga cagcagcgtc actttgtagg ccgcgaccac ctgcgggttg gtgatcacct
    60421 cccagtactg cgcccagctc atctcagaaa gctgcatcac cagcgcagag agcggcagca
    60481 gcaatatcag gcacacaaac agcagactgg tgccaaggct taaagtaaag cccggcagca
    60541 cgcgtcttga agaaactgca aacattactt acgccccgcc gctaacagct tgtctaactc
    60601 gccgccgctg gtgaagtggg ttttcatcac ttccggccat gagccaaatt tgtcttccac
    60661 gcggaacagt tcggtctgcg ggaatttatc tttcagtttg tccatgactt ccgggttatt
    60721 cacacggtag taatagtcgg tgatgatggt ctgcgcctgt gggctgtaga gccagttcag
    60781 gtaggctttt gcggcttttt ccgtaccgtt ggcctgcaca tttttatcga cccacgccac
    60841 cgggaattcc gccagaatat tggttttcgg gatcaccact tcaaagccct gtgcttcata
    60901 ctgtttacgg atgttgttca cttccgattc aaagctaatc agcacatcgc ccaagccacg
    60961 ctcggcaaaa gtggtggttg cgccacgacc gccggtatcg aacacttcaa cgtttttcag
    61021 gaactgggtc ataaactgtt cggttttggc tttatcgcca ccgtcagctt tatccgctgc
    61081 gccccatgcc gccagatagg tataacgcgc gttacccgac gttttcgggt tcgggaaaat
    61141 cagcttcacg tcggagcgca ccaggtcgtt ccagtcgtgg atattcttcg ggttgccctt
    61201 acgcaccagg aagcccatgg tggagtagaa cggcgagcta ttattcggca ggcgcgactg
    61261 ccagtcagcc gggatcagct tgcctttgtc gtgcaggatt tgtacgtcgg tcacctggtt
    61321 ataagtgaca acgtctgctt ttaagccctg caaaatcgcc agcgcctgtt ttgatgaccc
    61381 ggcatgagat tgttttatcg tcagtttgtc gccgccgtta tcttttgccc attgctgttc
    61441 aaacggcgga ttcagggcgg caaacagctc gcgggagacg tcataagaac tgttcagcag
    61501 ttccgttgcc tgtacatggc ccgccagcag cagagaagcg accagcgcga gtgagttctt
    61561 tttcagtaag ttaacggcca ttgcgcaccc ttataaattt aatgactttc taatagccat
    61621 catatttata acggacgtga aaggagtaac ggttttatat accgtttggt gatttggaag
    61681 ttaaaaaggg aataagaact tggttcaaca ggcgtaattg ttgcaggcag tttggacacg
    61741 gacagcgcgg agaaaccgga gcgtacacat agtacgtgag gatttcgagc actgcccggg
    61801 gccaaaatga caaatgaaat agcctggtga acttagttca agacgaaatc ctccccacac
    61861 atgcagggag gaaacagagg tgaatcagat accgacgcta accgtctccg gcagtgtgct
    61921 gccgccatca atcacattct gtgtaccggt taaatagctg gattcatccg atgcgaggaa
    61981 ggccgccagt tcgccgactt ccagcggatc ggcgaggcga cacagcggga ttgcttttgc
    62041 catttcagtc agcaccgatt ctggatcttc cgggttcgac tggcgggcaa tgctttccgc
    62101 cattggcgtg cggacgtatc ccgggcagat ggcgttaacg cgaataccgg attgcgcgta
    62161 ctccaccgcc agcgatttag tcaggccaac aatcgccgct ttcgttaagg catacgccgt
    62221 ttcgccagga tcggccacca tatcaccagt gactgaagac atcatcacaa tgcgaccatc
    62281 tttgcgcgca atcatctccg gcagcaccgc cttcgtgacg ttccatacgc ctttaatatt
    62341 gatatcaata tggaaatcgc gatcttcatc gctcatatcg aggaaactgc ccagacgaca
    62401 aacgcctgcg ttattcacca ggatatcaat gcgcccttct ttttccttcg cgcgtttgat
    62461 agctgcggct accgacgccg ggtcacgcac atcggcgaca accgccgtac agcgatgacc
    62521 acgaccacac agttcgtccg ctagcttttc gatctcaggg gagatatcca gcaagattag
    62581 gttcgcgcca tgacgtgcaa aagttctggc aattccttcg ccaattccct gcaatgcgcc
    62641 cgtaatcagt gctgtcttgc ccgtgagttt acccatttta atgctctcct tgttaataca
    62701 gagcgttaca ctccattccc tctgaaaata caggataaac aaaatattgc tgcggatcag
    62761 ctcactaatt caggaaaatc tcaatccagt cgctgcttta tgcatcattt caggcgttgt
    62821 gttagctcac tgctgcgctg aatcattttc aatgacgcca catcattgat gttgcacaag
    62881 accaacaaac agtaaatcgg taacagaatt ttgcgccgtg cgggtagaca tcgacgagct
    62941 acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa tgcgccagcc gccgcagcgg
    63001 agagtcagcc agagaagtaa tggcaatcac cgttgccccc tgctttcgtg ccgcttctgc
    63061 gcacaagaca atctcttttt tactgccgct gtaagagata gcgatttgta catcgccttt
    63121 tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtcg gcttcgcagg ccacgcgata
    63181 gccaattttc atcagtttga acgacagatc gcgccccacc agcgccgatc cgcccaggcc
    63241 cgttatctgg ataaacggtg ctttactgat gacctcaatg attttttgca gccgcgcgta
    63301 gtcgaacaac gcacaggttt gctccagcgc cagctctttt tcgcgattca gtttgcgggc
    63361 aatcacttcc agcgaatcat cactggtaat cgaactgtgc aggtgcagcg ccgtggcatt
    63421 cgttttctcc cggctggcac tgtattcgcc aattaacgcc atacgcaatt cagtaaatcc
    63481 ttgcgcgccg agcttctggg caaatttcac aatgctcgac tggctgatac cgagctgttt
    63541 cgccatctgc cgtgaagaga cggatttcag ctcgctcact ctggcccgca gaaagtcagc
    63601 aattttttgc tcgttcccgg taaattcact ttccgcattg cgtattttcg tcaggtacaa
    63661 catcttccac tctcctgggc tggcagcaaa aattccgctt aagttcttat gacgctcttc
    63721 acactctgcg agtgtagact caatgattcc tttaagtctg tcaattcggg atatatgatt
    63781 ccatacatct gcaattattt gatcgtaaat agtaaggtca ccaccgatgc aactagaaaa
    63841 gatgattact gaaggctcga acaccgcctc ggctgaaatt gaccgcgtat cgacgctgga
    63901 aatgtgccgg attatcaacg atgaagataa aaccgtaccg cctgccgttg atcgcgtcct
    63961 gccggatatc gccgcggcga tcgatgttat ccacgcccag gtcagcggcg gcggtcgtct
    64021 gatttatctc ggtgcgggaa catccggtcg tctggggatt ctggatgcca gcgaatgtcc
    64081 gcccacctac ggcgtgaaac cgggtctggt ggttggtttg attgctggag gcgaatatgc
    64141 cattcagcac gcggtggaag gagctgaaga tagccgggaa ggtggtgtta acgatctgaa
    64201 aaatattaat ttaacggcac aggatgtggt ggttggcatt gctgccagcg gtcgcacgcc
    64261 gtatgtgatt gccggactgg aatacgcacg ccagctcggc tgccgcacag tgggaatttc
    64321 ctgtaatccg gggagcgccg tttcaaccac cgctgagttt gccattacac cgattgtagg
    64381 tgccgaagtt gttaccggtt cttcgcggat gaaagcaggt acagcgcaga aactggtgct
    64441 caatatgctt tccaccgggc tgatgattaa atccggcaaa gtgttcggca acctgatggt
    64501 cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag gtcaatatcg ttaaaaacgc
    64561 caccggatgt aatgccgaac aagcggaagc ggcgttaatt gcctgcgagc gcaactgtaa
    64621 aacggccatt gtgatggtgc tgaaaaatct cgatgccgca gaagctaaaa aacgcctgga
    64681 tcaacacggc ggctttattc gtcaggtttt agacaaggaa taacccatgg ccaaagagat
    64741 cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc ggaccgggaa atatcgccag
    64801 ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat gacagttcac tggttgatcc
    64861 caatatcaaa acgctggaag gcgtgaaggg cgtcattttg accagcgacc aggtgcaggt
    64921 cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg atgagcgagc tgctgggcga
    64981 tgcaccggta caggatgccg cagaaatcgc cgcccagaac aaacgtcagt taaaagccaa
    65041 acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc accatcttca cgccgctgat
    65101 ccccggtttt attgccgccg gtctgctgct ggggatagcg acgttaattg ccacggtgat
    65161 gcacgttccg gcagatgctc agggcacgct acccgacgcg ctgaatttta tgaaggtgtt
    65221 cagcaaaggt ttgttcacct tcctagtgat tctggtgggc tataacgccg cccaggcatt
    65281 tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc tttttgctcg gttataaccc
    65341 cgctgcaacc accggttact acgccggttt tcacgatttc tttggtctgc ccatcgatcc
    65401 gcgcggcaat attatcggcg tgctgattgc cgcgtgggcc tgcgcccgca ttgaaggcat
    65461 ggtgcgccgc tttatgccgg acgatctcga catgctgctg acctcgttaa tcaccctgct
    65521 gatcaccgct acgcttgcgt acctgatcat tatgccgctg ggtggctggt tattcgaagg
    65581 tatgtcatgg ctgtttatgc acctgaacag taatccgttc ggttgtgcgg ttttagccgg
    65641 gctgttcctg atcgccgtgg tatttggcgt gcatcagggc tttattcctg tttacctcgc
    65701 gttaatggac agccagggat tcaacagctt atttcccatc ctttcaatgg caggcgcggg
    65761 ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa ccgcacagtg cgctacgcag
    65821 tcaggtacgc ggggcgatta ttcccggcct gctgggcgtt ggtgaaccgc tgatttacgg
    65881 tgtcaccctg ccccgcatga agccgtttgt taccgcctgt ttaggcggcg cggcgggcgg
    65941 tttgtttatc ggattgatag cctggtgggg tctgccgatg ggcttaaaca gcgcctttgg
    66001 gccgtctggt ctggtggcgc tgccgctgat gacttccgca caaggcatcc ttccggcaat
    66061 ggcggtttat gctggcggga ttctggtggc atgggtttgc gggtttattt tcaccacgct
    66121 ctttggctgc cgtaacgtca atctggactg atattatgaa acggacaatg ctctatcttt
    66181 ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata ccctgttctg acagaaggta
    66241 gttgatcaag tatatgcggc gttaaagcag taataaaaag gccagtcagt aatgttactg
    66301 cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcaacttgt ccagcgacgg
    66361 tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc aacatcgcat cacgtttgcc
    66421 atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta
    66481 ggcgcagaag tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctggcgaac
    66541 aatcttcagc cctttgccat cttctttcag atcaacgcgg gtgaggtgag aggtttccgg
    66601 acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg cgcccgatca tcatctcctg
    66661 atcgtgaacg ctcatccggt tgagctgctt caggttgtgc tcccaacgct gcacaaacac
    66721 atagctgccg ccggaatcca cgccgtcttt gataaccgcc acttcgcggc gcgtctcttc
    66781 acccgccggg ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt ccacccaacg
    66841 gaagccgtgg atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg
    66901 agcgacagag aagtttacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg
    66961 ggtcgtcggc gcaaggcctt taccgtagcc cggaaaatct ttcagctctt ctgccccaac
    67021 gccgccgctc agagcgcgcc aggtgttgtt accaaaggca accaccgcac caagatgcgc
    67081 gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg
    67141 cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc
    67201 tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataaga atgccacgca
    67261 atccgtggca ttgttgagcg ctattttacg tgttttttcg cgacggcggt ttgttcagac
    67321 gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa
    67381 ggcattaacc cttcaggacc acgccattcc ccgctgaact ggtagctaat atgttgactg
    67441 ccttcggctt tacattcgac ggcacgatta tcatcaccat cagctttttg gcaattgcca
    67501 aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt taacaccggt gtcagcagga
    67561 atatcgctat ccagcacgtc aatgcgactg atcgtgcctt gatcgccgtt aatcaccatc
    67621 gccacgttgt cgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgtggttttc
    67681 attccgctgc gcaggcgata atcgccatca agcgcatcag caatggcttg ttcttgcagt
    67741 ggtgtggacg ccgttaattc acccacgccc tgctcgctca ctttggtgga cgatccaaac
    67801 cagttccacg ggttagcggc agaccagtta actgacgaca tcgtggaaca gccggtcagc
    67861 atcaacggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt
    67921 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta
    67981 aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacaat atccgccact
    68041 accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccag
    68101 caaagtaaaa ccacctgcgc caacagcaac agaaaataga gcacatgcca ggtgcgagga
    68161 aacgaagccc gccgaccgct caacaaaaac gccagcaccg ccggaatacc gggaatcagc
    68221 cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctca
    68281 cgcgacgcac cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc
    68341 cagaagagca agggtaaacg caggcgtccg tgcgcatcat aatggacagg atggaactca
    68401 gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc
    68461 caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt
    68521 cgggcagccg cgagcaatca gctttttctc cagccggtta agcaacgcat tggcaattcc
    68581 acgcccacga aactctggat gcacgccaag ataataagca gacccgcgat gcccgtcata
    68641 accgcccatc accgttccga ccacttcacc gtttacctca gcgaccaaaa acaaactgac
    68701 gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac gcagcaagtc
    68761 gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc
    68821 catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt
    68881 agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg
    68941 aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta acggattacg cgatacgata
    69001 taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa
    69061 acactcactt cgcgccgcca ggtgctgaaa gccggtttag ctgccctgac gttgtcagga
    69121 atgtcgcaag ccattgccaa agacgaacct ttaaaaacca gcaacggaca cagcaagccg
    69181 aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg atccaggcca cggcggaatt
    69241 gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcgattgct
    69301 aaaaacgtcc gttccatttt gcgtaatcat gggattgatg cgcgtttaac gcgttctggc
    69361 gatacgttta tcccacttta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg
    69421 tttatgtcaa ttcatgccga tggctttacc aacccgaaag ctgccggtgc ttcggtattt
    69481 gccctctcta accgtggggc aagtagcgca atggcgaaat acctgtctga acgcgaaaac
    69541 cgcgccgatg aagttgccgg taaaaaggcg actgacaagg atcacctatt gcagcaagta
    69601 ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgcttgg ctcgcatatt
    69661 ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt
    69721 gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg aaacctcgtt tatcaccaac
    69781 ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc agaaaatcgc cacagcgatt
    69841 gctgaaggcg tgatcagtta tttccactgg ttcgacaacc agaaagcaca ttcgaaaaag
    69901 cgataagtta tgaaacccga cgcacaccag gttaaacagt ttctgctcaa ccttcaggat
    69961 acgatttgtc agcagctgac cgccgtcgat ggcgcagaat ttgtcgaaga tagttggcag
    70021 cgcgaagctg gcggcggcgg gcgcagtcgg gtgttgcgta atggtggtgt tttcgaacag
    70081 gcgggcgtca acttttctca cgtccacggt gaggcgatgc ctgcttccgc caccgctcat
    70141 cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg
    70201 cataacccgt atgttcccac cagccacgcg aatgtgcgtt tttttattgc cgaaaaaccg
    70261 ggtgcagagc ccgtttggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt
    70321 gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgccagcc atttggtgaa
    70381 gacgtttatc cccgttacaa aaagtggtgc gacgattact tctacctcaa acatcgcaac
    70441 gaacagcgcg gcattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac
    70501 tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta
    70561 gagcgacgta aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc
    70621 ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg cctgcaaact
    70681 ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat
    70741 tatcagccaa aagatggcag cccagaagcg gcgttaagtg agtttattaa ggtcagggat
    70801 tgggtgtaac tccctcaccc ccattcccgc atccgctgat gcaacgtcaa cgacggcttc
    70861 tcggcaaaca gctgctggta atccgtggca aattgcccca gatgccagaa tccccactgc
    70921 atggcggcgt cttttaccgt tgtgctttgc gaccacgggc ttatcagttc gcggcgtacg
    70981 acgttcaggc gaatgcgttt cagccacgcg tttgggccaa tgcctaaaat agcgtgaaac
    71041 gcgttttgta gcgtgcggcg actgacatgc agttgattac acaaatccag caccgtcacc
    71101 ggttcggaca tgttttccag cacatattca cgggcgcggg aaagcaaccg acggtaactc
    71161 tgatgactga tgctttccgc cgtcaccatt ggttgcgctt cttccagcat cgcccccatc
    71221 gccattagca aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg
    71281 ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagcgcggc tttatgctgc
    71341 tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc cggattgtgc
    71401 aaaaagttag cctgacaggt gatgacatct tcagacagca ctacgcccag gatcgtataa
    71461 tcgtccggcg tacttaattc aaactcggtt ccaccggggc gcgtcgctat ttctgcgctt
    71521 cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat gccaaaccag
    71581 aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccggtgta ttcacgaaaa
    71641 acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc
    71701 tgatcgtaaa tctgctgcta ggcggtaatc gtcaaagcat gttcatagac atccgttgtc
    71761 tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag
    71821 ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg
    71881 ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg ataacgacta
    71941 ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc
    72001 agcggccagc caaagtgggc ggccacttct cctgccgtca ttccctgaca tactgatgtt
    72061 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt
    72121 acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    72181 cgaccgatct ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    72241 tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttaa gcgaccaggg
    72301 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca
    72361 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    72421 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    72481 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    72541 tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg cagatttcaa cgcggcatca
    72601 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    72661 atcccggcgg ttgatgaaag ataagaaccg gtacgcgaaa ccacatgcgc caggaaggcg
    72721 gtgttttctg cgtcattggc ccactggaaa gctgcgccat tttcaatatg tgcaaccatc
    72781 gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca
    72841 ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc atataccact
    72901 tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt aggttacgtc gtcagaatcg
    72961 gcagaaatca gcccgaggct acgaatatgc ggcggcaatt ttagttcacg cgcaaattca
    73021 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    73081 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    73141 ttcgccaaat ccacaatgac ggcacagcag cacgaaagag aagcaaaagt cagctacctg
    73201 gtacactagg cagcgggtca gcaaaggggg gcaaatgccc cttttttcat gtcagataat
    73261 cagatcatta cctcaataaa ctaataatca catcgcggtt acaaccagtt tgatagtgat
    73321 agctcaaacc cagacgtggc gctggctgca agcaaaataa gccgcaaaaa tgcgacccca
    73381 tgtttactag ccgcaaaaat gcggtcctat ttgccagaac cttttattat tcaattggtt
    73441 agtgcaagaa aaagcgtgat tgctgttcaa gaagtatcca gaaaggtcat gattttgacg
    73501 ctgaaaaaaa ataggtgggc atttttgccc actcactagc cgcaaatttg cccactcact
    73561 agccgcattt ttgcggccca aagtaataaa tacagagaaa taataaatac agatcagcgc
    73621 caagattttt cgtcttttcg gcggaatcat gagactagtg cttacgtcct gataacctcc
    73681 agagcagacc ttaacttctg tgcagcagga ctacgctcat tgccgctcgg ctaggtcaaa
    73741 tcctcacgat aaagctgttc ggattgtgac cataatgtta aaggactctt taaatcattc
    73801 cttgacgaat taactagctg gcttaagcgg aatagcatgc aggataaaaa caaaaccagc
    73861 ttagcagtgg ggagagcttc gcttcgctcg tctcctgggg ccaatgctca atgaaacgac
    73921 gtaaggcacg gagcgctcac cataggtaga cacataaaca tctgagagcg caccttctat
    73981 cgcgctacgc gctcaataaa aaccaacgac accgccacac tgcaagacca atggttttta
    74041 tcgcgtatac agaccagaca ggaaacagca gcaaagcagc gtatagccag gtgcagacag
    74101 cacactcaca cgacgcacag caggcttaag tgatcactca ggaaacttta tccccgccgc
    74161 cgatcaccgc gaactatcat gaccgcgcca aattcgaaag gaatggccca tagaacgcga
    74221 tgaacacaca taagcgctac acttacttaa cgtcgccaca gagcgattga gagcgcttaa
    74281 aatcgatgcg atttcattaa atttttctca ctcgcctgaa tggtggcgat caggactgat
    74341 cgcaacattg ccccgcctcg atattgagcg cataatagat acatacatta agcggaaaag
    74401 agataagttt attgtgaaaa tcagggggtt acatatcatg cgcatatcag aacaacaacg
    74461 gcagatcctc gactatctgg caaaaaacga atcaatcacc gcacgacaag ccgcagatct
    74521 cgtttatggt ggcaatgtta cccgcgtcca ggttgacgcc gcccgccgct cactcatgac
    74581 gatggttagt aatgggctaa tcaggaaaca aggccgcgct ttcgtagctg aaagacatat
    74641 caatcacgct tatagccagg acgttgaaga attgcgccgc cagatccttt gggtattcag
    74701 caaacagggt caggagtatt tcctttctga acacccaaat ttcgccgcca aagagttaac
    74761 agtaagcgga ttgtcgtgcg cactggatta tcttgtcggt gaaggttgcg ttacttacaa
    74821 gcagctatgg cacgccctga atacgcttgt agaagatggt ttactcattg ccacgcgtga
    74881 gaacatcgaa tactggataa tgccgcgttc aggtcgccgc aatcatgcac ggtatatttc
    74941 aggcaacaga tacagaacgc cggactttaa tccgacacag gaagatcggg accgtgccga
    75001 aatgatgtgc agggaacagg agaaaagaat tatggataag ttattttcga tgcagtgaac
    75061 acaagcgggc gcaatgcccg tttttattgc cttattccac gcctgacgaa tcaatttgat
    75121 cacacctgag aaaatgcata gccagaagtt aaggccgtta tattgcgatt gggagcgttt
    75181 taaacggtat caatttaatt aattctgatg tggtgatttg caggcaaaaa aaacccgccg
    75241 gagaagcggg aaatagagac atatcgttgt agttattaac agatcacttt gaggtcgagg
    75301 gcggctaacc ctcgctaata ttatcccgct attaccttgc gggtgcaaat aatcttttat
    75361 tgctttgatg atttaataaa tagcactttt tgctaacaaa aaccgctgtc aatatatggc
    75421 caaaaatgtc aacagaaaaa ataaggttta taatattaag tatcacatgg gtaatgggtt
    75481 cagggaatcg gaatccaccg cctcacggaa attcaaaatt tgcttcttct ttcctccgtg
    75541 ggcggcttct ttttcgtatc gaggtattta tgaaagcaat aacattagaa cagttcgcag
    75601 caatggcgaa tgtatcgacc agaacgctga ggcgctggct aaaaaaacca tcaaccggag
    75661 tccctttgcc atttacccca cgcggtactc gccaattatt ctgggagcac gaatgcaaag
    75721 aatggttgta cagaacagaa cgataataat cacgcatcac attttgaggt aaacatcatg
    75781 accaatatcg aaagattaga acgctgtgcc gatattatgc gacgcagatg gatttatgac
    75841 cctgaagcgg gtttattgtt tagccgtgaa acaaaagacg ttatcaaagg cagcatgacc
    75901 aataaagggc atttggttgt aacggtacat gaaggtaaat tcaaagtgac actcacctat
    75961 cagaaagcct gctatgtata cacatacggc gcttatgacg agacattata tgaagtgcac
    76021 cacgttaatt tgaacaaaca ggataaccgc atcaagaata ttcgcctgat gccgaaagaa
    76081 aagcaccgcc ggatacataa aaacgtccgc aaacttattc ggctgggcca cacacaatgg
    76141 cttcaaccgc aattaattga acaatcacta taaacacagg agtaaatacc atgcattcat
    76201 tagacagtta ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa cgccgtgagg
    76261 aattccatga gaaagtgatg cgttctgctg attatattgc ggacaagttc gtggaaacgg
    76321 ttcgtccact tgttgatgaa gtagctgata aacttcagtc tgaaatgccg gaagatatgg
    76381 aaggcactgc aaagcggcga ctcatttgcg aattaagtcg tcgttttggc gtgtcaataa
    76441 gcgcttttaa ataatcgaaa aaagggggga aagataatgg acgcatttga ggtactggca
    76501 aataaaggcg ctgaactggt agcacagcgt gataaaaccg ccaacgaagg ggaacgcgca
    76561 ctgctaaata aacagattaa agcgatccgt atggcccagt ttaaattaat cagtaatgaa
    76621 gttatcgaaa caattaaacc gcagatccca caaatcgttg ctgacgctat taaagccgcc
    76681 ggactggaga aacgcattgc cgggctaaaa acgaacgcag agaaaatgga gatgtacaaa
    76741 cagagcggtg ctccgaatcc cgatgagtac ttctcaccgg atgttgagtt tatggcgcag
    76801 gttgaggaac gtctgggtag ctgtctcacc gaggaacagc gtcgctattt tgatggagtg
    76861 gatagttcgg caggtattga cctgaactcg tatttcggtc gtgaaattga gcattttgat
    76921 gccgccccgt ctatgccgga gccagaacca gaaatggcca ccgatcctga agctgagcaa
    76981 cggaaacgcg tattcaatgc gatagggatt cggtactgat cggataaaag aaagccggga
    77041 attaccccgg ctgacttaac aatgtgacca atgaacaaat cggacaagac aaacaggata
    77101 agcagtctcg aaacaatgtt cgggaactat attagcacaa gtccggagaa aatcatcatg
    77161 ttggcactac gcatcatgca gggcatcgct aagaccctcg cagaacatgt cttagacctg
    77221 aaacattcgc cactgagcaa acaggccatg aagcgccaaa cgcttcgtct gtgggctgaa
    77281 tattcgcttg gaactatcaa taaaatcatc gacatgaaat cggggccgag caaccagagc
    77341 gcggaggaaa tggagttcat caggcgttta attctcattc gcagagacat tcatagccag
    77401 ttacacagcg tgggaatcga tattaatgat gggacgggcg attaattttc ggacaagtac
    77461 tatgcgcgcg cgaggagctg aacatgaagc aagatattgc agacaggctg gagatactcg
    77521 aaggccagcg ggcagaagcg aaacaactac gtaagcaggc cagaagagcg cacaggaaca
    77581 acgaagcaga gttactaacc aaatacatca gtttcaccaa ttactgcatc tacgagtgtt
    77641 acaaggaaga tgcagaggac tggctggatt cattgccaga acagtactga gcagaataac
    77701 accacgtcat gttgttctga ggtgagcata tcacctctgt tcaggtggcc aaatttagtg
    77761 tgccacttca gcatcaccgt tgaaatcaca ataacgattt ttttgtaaat atgctctcac
    77821 atatgtagga atagaccagg ttggcattga aacacatcta ccatgaccat aataaagtaa
    77881 ttttttgata tgtttatggt tttatattaa ttaaaatata tctaaatttg atagttttaa
    77941 aattgcaaaa tccagattgt gtttgtaatg tttaatctga ttgtattgag tggcgtttat
    78001 tatgaaaaat aaatcaggta attcttgtta tagagaagca gtcgatattc atagctcact
    78061 tgagctttca tttatattgc ataaatatca tttgcgtgta gatgagaaga aatctcgaaa
    78121 tgtattcagc gccatacgtg atgagctcat ccttgatggg gattcacgac aaaacttagc
    78181 tactttctgt caaacatggc ttgatgatga aatccgtgag ttgcttaatt tagctattga
    78241 taaaaacatg atttataaag atgaatatcc acaaatggcg gaacctgaag gtcgctgtgt
    78301 gcgtatgctt tccgattcgt ggagtttccc cgattcgcga cacaccttag gttgctcaac
    78361 cattggttca tctgaagctg ctatgctcgg ccgcttggcg ttaaaatggc agtggtgtaa
    78421 aaaacgcgaa gtacaaggta aatcaaccga acctgatttg cgatcctgtg caaatatgct
    78481 ggcataaatt ctgccgttac tgtgatgtga aattgtgtga aattccactt gatgatcatc
    78541 acctgataat gaatgctaaa gaggttttga agcatgtcga tgaaaacact attggtgtcg
    78601 ttacgacttt aggtattact tatttttgta aagcagattg cgttagtgtt tttaagataa
    78661 gtgttaaagt taatattagt ttttatattt acatcgtaat tatatgtttc tattttaaaa
    78721 atgttcgaga gtataaaaat gtctaatgct aatatcgcac ccaaaagtaa gaaaatgaca
    78781 gtggggacac tcgctatcat gaatataact gctgtagtaa gtctacgatt tataccttct
    78841 gaagcagaat acggcttagg tgccattttt tactatacct ttgctgcaat tatgttttta
    78901 gtacctgttg cctttgttgc ggctgagcta gctacaacat atccacaaaa agggggggca
    78961 tttcgatgga taggagaagc atttggggct cgctggggtt ttgtaggaat gcttatgacc
    79021 tggctacaag tcatccccta ctttccaaca gtactgacat ttggtgcagt ctcggttgca
    79081 tttattgatg ttgatatggg ggtggcagaa tcaattgcca gtaataaatg gtatatttta
    79141 tttttcgtac tatttgttta ctggggagct atttgtgttg cattacgtgg cgttggtatt
    79201 ttctcgcagg tatctaagtg gtgcggtatt atagggacaa ttattccagc tattatcctt
    79261 gttattcttg gtttctccta tctgtggttc agcggtaaac cagcggtgat cccattaggt
    79321 tggggtgatc ttttccctaa tatagctaac ttccagaata ttgttcttgc cgccagcata
    79381 tttcttgctt atgcagggat ggaaatgaat gcggtccatg ttaacgattt agataatcca
    79441 actaaaaaat acccaattgc aattaccatc gcttctatag gtactattgc aatattcctg
    79501 ctcagtactt taggggtcgc ctttattatt cctacagata aaattagtct aactcaaagt
    79561 ttgttggttg cctacgatat gctgttcgaa tgggctggag tgccatggct tggctctgtg
    79621 atggcattta tgctagcaat tggcgtactg ggtggggtgg ttacttggat tgcagggcct
    79681 aatacaggtg tattagcgat cgcgaaagct ggttatttac ctaagttttt ccaaaaaacc
    79741 aatcgtcatg gaatggggca ccatctaatg tttgtacaag gtataattgt ctcagttctt
    79801 tctgttactt ttgtgataat gccttcagtt caagcagctt ttcagatttt atcacagtta
    79861 acagttatgc tctatttagt tatgtatatg ctcatgttcg ctagtgctat ctatctacga
    79921 cattctcaac ctgatacaaa acgcccatat cgagcccctg ctttattttt ctgggcaaca
    79981 cttggattct tagggtcttt tctggctttt gtattaagtt ttattccgcc cgagcaaatc
    80041 ccaactggta gtccaaccag ctatgttctt tatttgttag tattagttgc cctattttta
    80101 ttaattccaa tattaattta cgccttccga aaatctgact ggaaagatcc aaactctgat
    80161 ttcgaaccat tttcttggga aaaaagaact agtaattaaa ttttccataa gctacaaaag
    80221 atgaggttaa atatgtttag tgctgaatct ccaattggtg taatagactc aggtgtcggt
    80281 ggattgacat cggtaaagga aataattaat ttgcttcctg gtgaagatat tatctattgt
    80341 ggagataata tgaatgcacc ttatggcaac cgttctgccg atgatatcat cagtttgact
    80401 aaaaaaatgc tgactttttt acagtcaagg aatgtcaaac tagttgctgt cgcatgtaat
    80461 actatatcct caactttgga atccgaagaa tacagcggat atgctaagag ctttccgttt
    80521 cctatcttat ccatcattga acctgcagta gaagatgtga ttcgccagca atataaaaat
    80581 gttggtatta tcgctaccga gtttactatt aaaacggggt gtcacaaaga gctaataaaa
    80641 aaattgaatt caacaataaa tgtatttgga gaaccaagca aaaatcttgc aatgttaatt
    80701 gaggaaggta atcttaatgc tcctgcaatc ttgaatgaca tcaaaaaaca tgttaaccat
    80761 ttaatttccc tccatccagt aaatgaaatt attctcggct gtactcacta tccaattgtt
    80821 cagaattttt ttgaagcagt tgcaccggat ataaaattta ttaatcctgc acatgatcag
    80881 gctatctcta ttaaaaatca cttgggacag ctaaatttat taaactcctc aaatatagga
    80941 actttgcaaa taaatactag tggaagtatg gagatctaca aaacggtact aggggaattg
    81001 agcattacga aacctcatac attcagtatc aggcagtttt aaatttatcc ggtacaccgt
    81061 aaaatcccgt ccttctgaac tgcaccccaa aagttggaca ccaactgagt aaggtgcatt
    81121 ttatggacaa acccagttac atactgggta gtgcatccaa ttagtagaac atgtgttttt
    81181 cgataaacgc tccgatcact ttttcgtgga tctcaactga gcgtgagaag cagattgttt
    81241 tacgacccaa ccgcttaatg cgggtgcgta gcgtcagatt attacgctca atgcgttggg
    81301 tgaatatttt gccggtcaga tgcttattct tcggcacctc ccggccatag ctgccccagt
    81361 catcgctggt cagcatgccg atgttgaagg gtgtaagcag tgccagtagc tcccggcacg
    81421 tttgatcggt tcggggacca aaagtgtagg ccagtacacc gcctgttttg gtgttgtacg
    81481 cgtacccgag ccaatgttgc cgggctttac tgccaacgta gctccattgc tcatcaagct
    81541 cgcagataaa cgccacatca gcatgggcaa caggcgaaga agttattcgc tttggcgcga
    81601 gttttttaaa gtccggatga cggtgttact accaattttc agtgtcctgg cggtatcgcg
    81661 aaccccggca ccattaaagg ccatttcagt tatcagctct ttcatacccg gcttacgtgc
    81721 ttgataagta taagtgaact gaaacacacg gtggcagtca cggcagcgaa gtctgtcacg
    81781 gcctttaggg ttctgaccat ggcggtaaac ctgagctgac tgacaacggg gacaatgaat
    81841 attaacgctg gtcatgagat aacctcaaaa gcccgtatta tacatcagat tcaactaatt
    81901 agaggcatca cccatccagc attataaaac atactaacaa taactaaatt ataataatca
    81961 caacttacaa tcaaataata aatgcataag attaacaatg attttttcgg gttatattta
    82021 tctaaaaaag cagaatatta tgtaacttat tattttctaa gcgaaaattt attgtgcggt
    82081 cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta ttctttcttt
    82141 tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctgttc tggttttggt
    82201 tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc
    82261 accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga
    82321 tataatccca ctaacaccca gacaccaagc accatacctg ataactaccg tattcaggaa
    82381 ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca
    82441 atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt
    82501 tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc
    82561 agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc
    82621 agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cacgtcagtt
    82681 ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca
    82741 tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt
    82801 gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtgga gcgtacggtt
    82861 tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc
    82921 agaagtgggc caatatcggc aaaacagaac gggttggcgg gctggtggcg ataacattcg
    82981 cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt
    83041 tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc
    83101 gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct
    83161 gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc
    83221 tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt
    83281 ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga
    83341 cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg
    83401 aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc cttcatcact
    83461 ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg
    83521 atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc gggcacggac
    83581 gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag atccgccact
    83641 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
    83701 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    83761 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    83821 tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg ttgcggcgac
    83881 gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
    83941 atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga catagatcag
    84001 catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
    84061 ttcacccggc gtatcaatat cgcccttatc attaaattcc acggccagtg tttttctggc
    84121 gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacgctacc
    84181 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
    84241 ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc
    84301 caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga tatgcacatc
    84361 agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag ttagcgtcat
    84421 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct caccgggatg
    84481 cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    84541 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    84601 gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcaata agaatggaga
    84661 gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat aaaataatat
    84721 cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa gctttgctaa
    84781 tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag ttaagaatga
    84841 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg acgatacgac
    84901 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
    84961 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
    85021 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
    85081 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
    85141 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
    85201 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
    85261 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
    85321 tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgca ttcattgcac
    85381 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacacta aaatgttcat
    85441 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
    85501 cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga gtgacaccgc
    85561 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
    85621 tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca
    85681 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
    85741 cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg cgcttcggcg
    85801 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
    85861 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
    85921 tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
    85981 gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa gccaccactt
    86041 cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt
    86101 cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcaaagaa
    86161 cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca tccggacgca
    86221 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
    86281 gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccca
    86341 ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg
    86401 catatgcggc tttggtttcc gccatatttg gctcacggcc ctgatagata acgccttttg
    86461 aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg ttcatacagg
    86521 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    86581 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    86641 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    86701 gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg gcgacgactt
    86761 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccagcga
    86821 atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt
    86881 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta ccgcgcgcgg
    86941 tatatttgta ggcttttagc gggtcttttt cgatttcaag acaaggtgcg gcaacgcctc
    87001 tcttttgata aagaaaagag tattgagaag gcgcgccatc tggtggactt gtatcagcaa
    87061 caaggcgttg agaaatcacg cattttgatc aaactggctt cgacctggga aggaattcgc
    87121 gcggccgaag agctggaaaa agaaggtatt aactgcaacc tgacgctgct gttttctttt
    87181 acccaggcgc gggcctgtgc ggaagcaggc gtttttctga tttcgccgtt tgtagggcgt
    87241 atttatgact ggtatcaggc acgcaagccg atggacccgt atgtggtgga agaagatccg
    87301 ggcgttaaat cggtgcgcaa tatctacgac tactataagc aacaccacta tgaaaccatt
    87361 gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg ctgcgatcga
    87421 ctgactatcg caccgaattt gctgaaggag ctgcaggaaa aagtttctcc agtggtacgt
    87481 aaattaatcc cgccttctca gacgttccca cgcccagcac ccatgagcga agcggagttc
    87541 cgttgggagc acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg cattcgtctg
    87601 ttcgctgttg atcaacgcaa actggaagat cttcttgccg ccaaactata aaccagccac
    87661 ggagtgttat atgtcccgaa aagaccttgc caatgcgatt cgcgcactca gtatggatgc
    87721 tgtacaaaaa gccaattctg gtcatcccgg cgcgccgatg ggcatggctg atattgccga
    87781 agtgctgtgg aacgattttc ttaaacataa tcctaccgac ccaacctggt atgatcgcga
    87841 ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc tacatctgac
    87901 cggttacgac ctgccgctgg aagaactgaa gaacttccgt cagttgcatt cgaaaacccc
    87961 aggccacccg gagattggct atacgccagg cgttgaaacc accaccggcc cgcttggaca
    88021 aggtttggcg aacgccgtcg ggctggcgat agcagagcgt acactggcgg cgcagtttaa
    88081 ccagccagac catgagatcg tcgatcactt cacctatgtg tttatgggcg acggctgcct
    88141 gatggaaggt atttcccacg aagtctgttc gctggcaggc acgctgggac taggcaagct
    88201 gattggtttt tacgatcaca acggtatttc catcgacggt gaaacagaag gctggtttac
    88261 cgacgatacg gcaaaacgtt ttgaagccta tcactggcat gtgatccatg aaatcgacgg
    88321 tcacgatccg caggcggtga aggaagcgat ccttgaagcg caaagcgtga aagataagcc
    88381 gtcgctgatt atctgccgta cggtgattgg ctttggttcg ccgaataaag caggtaagga
    88441 agaggcgcac ggcgcaccac tgggggaaga agaagtggcg ctggcacggc aaaaactggg
    88501 ctggcaccat ccgccatttg agatccctaa agagatttat cacgcctggg atgcccgtga
    88561 aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt gccgcctata aaaaggctca
    88621 tccgcaactg gcagaagagt ttccccgacg gatgagcggt ggtttaccga aggactggga
    88681 gaaaacgact cagaaatata tcaatgagtt acaggcaaat ccggcgaaaa tcgctacccg
    88741 taaggcttcg caaaatacgc ttaacgctta cgggccgatg ctgcctgagt tgctcggcgg
    88801 ttcggcggat ctggctccca gcaacctgac catctggaaa ggttctgttt cgctgaagga
    88861 agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa tttggcatga ccgctatcgc
    88921 caacggcatc gcgcaccacg gcggctttgt gccgtatacc gcgacgttcc tgatgtttgt
    88981 tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg aaagcgcggc agattatggt
    89041 ttatacccac gactcaattg gcctgggcga agatggtccg acgcaccagg ctgttgagca
    89101 actggccagc ctgcgcttaa cgccaaattt cagcacctgg cgaccgtgcg atcagatgga
    89161 agcggcggtg ggctggaagc tggcggttga gcgccacaac ggaccgacgg cactgatcct
    89221 ctcaaggcag aatctggccc aggtggaacg tacgccggat caggttaaag agattgctcg
    89281 tggcggttat gtgctgaaag acagcggcgg taagccagat attattctga ttgccaccgg
    89341 ttcagagatg gaaattaccc tgcaagcggc agagaaatta gcaggagaag gtcgcaatgt
    89401 acgcgtagtt tccctgccct cgaccgatat tttcgacgcc caggatgagg aatatcggga
    89461 gtcggtgttg ccttctaacg ttgcggctcg cgtggcggtg gaagcaggta ttgccgatta
    89521 ctggtacaag tatgttggtc tgaaaggggc aattgtcggg atgacgggtt acggggaatc
    89581 tgctccggcg gataagctgt tcccgttctt tggctttacc gccgagaata ttgtggcaaa
    89641 agcgcataag gtgctgggag tgaaaggtgc ctgatggtga tcgccggatg ctgtatgttc
    89701 ccctcaccct aaccctctcc ccagaagggc gaggggacgg tccgtgcgct ttttagggcc
    89761 gcatctgcga ttttgtttaa cgggtgatcc acaacgtggg cctggcgtct ggcccatgcc
    89821 agttatcgca ggtaggttct gcggcgtaac gcaccaggcg aaaacgctga ccgtcaaagc
    89881 gccagcgcgc ctgaatgcca caatcgctta atccgcgccc tttcgctaag gtcaccagtt
    89941 cacgcgattt ctcatcaaat gttgcgttca tcagttccag ttcattcgtc tcctggccgt
    90001 tgttgaacgg caaacgcaac cgaaccgggc gcgaggctag cggcttttta cgcgacacaa
    90061 tccatgccaa atcaatggtg ttataggccc ctgcctcaca gctaatcatc atcagtgctt
    90121 tatcatcggt cagcgcagtg acattcacct cacgacgtaa cggatcaagc gagcagcgca
    90181 gaccattcat ccgccagttt ccataatcca gcaaatcgtt acgttcttcg agtgagagtg
    90241 gcgtcggcgt tgggttaacc accgcgacct ctttcagcgc aggcgcgggc ggtacgctga
    90301 gaggcggttc gtcccctttc ttgatccacg cggtttcact gccaacgcgc ttttgctgag
    90361 catcaataaa caacaacgcc gcttttaagc cgctcagaga aatggtctga ttgccgtcgc
    90421 gtaaggtgat cgccttccct tcctgaatta tctgcaaaaa cgcggtgatg gttgccgtat
    90481 catcggttac taataaccat ggtgaaatcc gccacttgtc accacttaat gccaacggct
    90541 caccatctaa tagcagccgt ggcgctatct ccccttcaga cgcatccggc gacttcaacc
    90601 caccgcgctc aatacgtaaa acggcatcgg tatgcgcccc agcgctgcgg ctcagggtca
    90661 tcaccagtcc attatgatcg cccgtattgc gcgccacaca aaaattttga ttattacagg
    90721 tgacctgcca gtcggaaaac gcccgttgcg ctggtgccgc ccacaccaaa gacgtcggca
    90781 acaaagcgaa aaaaaagaga aggaaaatgc gatagcgcat ggacggtacg accccagaag
    90841 ttcaaacaaa cagcacaagt cgtatcttcg ttgcgcaggt agcgcggctc aatcggattt
    90901 gtcggattga gccaataata aacaacgaaa tattattgct atttttcagt ccattaaatg
    90961 tgacatttgc aaatagttaa gcaataacac cgtcttaccg tcacgtatct cgccggtttt
    91021 gatcatctcc agcgcctggc tgaacggcag ctcgagcact tcaatatctt catcttcaac
    91081 accgccaccg gcgttagcgc gctgattgtc actgtattcg gcgataaaaa agtggattag
    91141 ctcagtcaca ccgccaggcg acatatacag ttcaaataat ttgcgcactt cgcccacttc
    91201 atagcccgtc tcttcaatcg cttctttacg aatgcacact tccggttcgt cgttatccag
    91261 cagcccggcg caggtttcaa tcagctgccc gctttcattg ccattaaccc aggtagcgac
    91321 acggaactga cgaatcagaa ccacgctctt tttcttcgcg ttgtacagga ggatcgtcgc
    91381 accattaccg cgatcgtaaa cttcacgttt atggcggata acttcgccat ctttgcgcgt
    91441 gagatcgtaa gtaatgttgt gcagggtgaa atagttatcg gagagaattt tgtctttaat
    91501 gagggtgatt tgttgcgtca taccgactcc acagcgcgaa atgaacaatt atattacgct
    91561 gtgaagtcgg gtttgtctgc gcaggctatc agtgagattt gactgatttt acccccagcc
    91621 agtcaataat cccctgtgcc gcatgacgtc cttctgccat tgcggtaacc accagatccg
    91681 caccacgcac ggcgtcgcca ccggcgaaga ttttcggatt ggtggtctgg taacggtact
    91741 ggctttccac atccgcgatg atgcggcccc atttgtctac cgttacaccg tgcgactcca
    91801 gccacggcat cccgtgtgga ttgaagccaa acgccataat caccgcatcg gctggcatga
    91861 caaattcact gccctcaatc ggtacaggtc gccgacgccc ctgggcatcc ggctcgccaa
    91921 gacgcgtgcg caggaaacga attccgcaaa cgtgaccttg ctcattcagc tcaagcgcca
    91981 ccggctggac gttaaattcg aagttggccc cctcttcgcg ggcgttcttc acctctttct
    92041 tcgagcctgg catgttagct tcatcacgac gataagcgca ggtgacgtta ctcgcgccgt
    92101 ggcgcagcgc agtacgcaca cagtccatcg cggtgtcgcc gccgcccagt accacgacgt
    92161 taagtccggc ggtattgata aacggctctt ccggtagctc ttcgagcccc atcacctgtt
    92221 tagtgttggc aatgaggaac ggcagcgcgt cataaacgcc cggcgcatct tcattgggta
    92281 aacccgcttt catggaacgg taagtgccta cgccaacgaa gaccgcgtcg tattgttcca
    92341 aaagcaaatc caaagagaca tctttaccca cttcacaatt gagttcgaag tgaatcccca
    92401 tcgcgctgaa gatttcccga cggcgtgcca gcagggattt atccagtttg aaagaaggaa
    92461 tgccgaaagt gagcaagcca ccgatttctg gatggcgatc gtacaccgtc accgccacgc
    92521 cattgcgggt cagaacatcc gcacaggcca gccctgccgg acctgccccg ataatcgcca
    92581 cccgcttgtc cactttggtg acatgactta agtcaggacg ccaacctttc gccaacgcct
    92641 gatctgaaat atagcgttca atgttgccga tagttaccgc gccgtgctca tcgcgaatag
    92701 tacaggcacc ttcacacaaa cggtcttgcg gacaaacgcg tccggtaatt tccggcaggg
    92761 tgttggtctg gtgagaaagc tcgacggcgg cgtcgatgtt tccggctttc accagttcaa
    92821 tccactgcgg aatatggtta tgcagcgggc aggtccattc acaaacgctg tgctcgccga
    92881 acttaaggca gcgcgaggct tcccgttgtg cctggtcggc gcgaaatggc agataaattt
    92941 catcaaaacc ggttttgcgc gcttcaatcg ccagtttatc cggctcgcca cgcgcgggcg
    93001 ttgcctgcat ttgttcgact ttactcatta ccggcatttc ttgtgcagcg gtactggcat
    93061 gccacggttg atgctcctga cgtgcggtgc gcaagcggcg ggatttcgcc atgccggaga
    93121 gtgcgacgtc agtgaccagt tgcagcgcgt ccgccgggca attctcgaca caggcaggac
    93181 cgttttcgcg ccctgcacaa aggtcgcatt tatgcgccgt ggcttttact tttcctgccg
    93241 cgacgggtgt caggacgatt tgcatcgtac caaacggaca ggccaccacg caggatttac
    93301 agccaataca cttttgctga ttgacctgaa tgctgtcatc aacgtggctg attgcgccat
    93361 tagggcagct acgggcgcag ggcgcatctt cacaatggtg acaggtcact gcactacgtt
    93421 gctgctgatg tttgataacc gtaattcggg gatgaaaatg gtgttggctc aggacatgtt
    93481 gctcatcatt gtgagccatg acacaggcga tttcacaagc atgacaaccc agacattgct
    93541 gactgttggc cataataaaa cgattcataa cgaccttctt ttttggttgt aaaaacctta
    93601 ttctttatat gagtgttgtt attacccgac ttacagggga accggcaatg ttcatgtgcc
    93661 cagaataagt aactatttcg ctataaactg tggcagatca aataatccca tcactgacta
    93721 aattgcgttt cagcgaactg gaacattaat gattttttgt ggagaagacg cgtgtgattg
    93781 ttaaacgacc cgtctcggcc agtctggccc gggccttttt ttacattgtg ctgctgtcga
    93841 ttctttccac gggtatcgct ctgctaactc tggcgagcag tttgcgcgac gctgaggcta
    93901 tcaatattgc cggatcgctg cgtatgcaga gttaccgcct gggctacgac ttgcaaagtg
    93961 gcagtccaca actcaatgca catcgccagt tgtttcagca ggcactgcat tcaccggtat
    94021 taaccaacct caacgtctgg tatgtgccag aagcagtaaa aactcgctat gcgcatctga
    94081 atgccaactg gctggagatg aataatcggc tcagcaaggg cgatttgccg tggtatcagg
    94141 ccaatattaa taattatgtt aatcagatag acctgttcgt gctggcttta cagcactacg
    94201 ctgaacgcaa aatgctgctg gtggtggcga tttccctggc tggcggtatc ggtattttca
    94261 cgctggtctt ttttactttg cgccgcatac gccatcaggt ggttgccccg ctgaatcagc
    94321 tggttaccgc cagtcagcgt attgaacacg ggcagttcga ctcgccgccg ctggatacca
    94381 gtctgccgaa tgagcttggt ctgcttgcaa aaacctttaa ccaaatgtcg agcgagctgc
    94441 ataaattgta cctttcgctg gaggcgtcag tagaagaaaa gacccgcgat ctccacgaga
    94501 ccaagcgtcg tctggaggtg ttgtatcagt gcagtgttcc caggcgctga acaccagcca
    94561 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcagctga
    94621 atatctggag ctgaacgttg gggataactg gcggattagc gaagggaagc caaaccctga
    94681 gctgccaatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    94741 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agtgtttcat cgatgctggg
    94801 acgcggtttg tactttaatc aggcgcagaa gcattttcag cagttattgt tgatggaaga
    94861 acgtgcgacc atcgcccgcg aattgcacga ctcgctggct caggtgcttt cttacttacg
    94921 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    94981 catggccgat ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    95041 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    95101 tacgttacaa aatcaaacca gcgctaaact gaccctcgac tgccgtctgc caaccctggc
    95161 actggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    95221 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    95281 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    95341 tcattatgga ctgaatatca tgcgcgaacg cgcggaacgg ctgggcggga cgctgacttt
    95401 ttcacaacct tccggcggcg gcacgttagt gagtattagc tttcgctccg cagagggtga
    95461 ggggaagggg aacacgccgg ctgaggtgta aataattacc ctcacaccgg ccctctccct
    95521 ttaaaggcga gagggaaaaa cgtgcccata ttgacctgta agcgagtgtg tcagcaatct
    95581 catgataatt tacattaact cctttttttc tccacgattg gctcgtacct tgccgctaca
    95641 gtgaagcaag tcaagcctac aacaatacgc agaaacacga ggtcctcttt taatggcgaa
    95701 tttctttatt gatcgcccca tttttgcctg ggtgctgaca atcctgttgt gtctgacagg
    95761 taccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc caccgaatgt
    95821 gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca ccgtgaccca
    95881 ggttatcgag caaaatatga ccggcctcga taatctgatg tatatgtcat ctcagagcag
    95941 tggcaccggt caggcatctg tcactttaag ttttaaagca ggcaccgatc cggacgaagc
    96001 cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc aggcggtgca
    96061 aaatcagggc gtgacggtgc gtaaaaccgg cgataccaac attctgacca ttgccttcgt
    96121 ctctaccgat ggctcgatgg ataaacagga tattgctgat tatgttgcca gtaatattca
    96181 ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt cgcaatattc
    96241 catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg ctaaagatgt
    96301 cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg gtggtacacc
    96361 ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc tgcaaacacc
    96421 agaacagttc cgcgatatca ccttgcgggt gaatcaggac ggctcagagg taaggctggg
    96481 cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc gcttcaatgg
    96541 taagccagcc tccgggttgg gggtaaaact ggcctccagc gcaaacgaaa tggcgacagc
    96601 ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtat ttcccgcacg gactggaata
    96661 caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg tggtgaaaac
    96721 gctgctggaa gctatcgctc tggttttcct cgttatgtat ctgttcctgc aaaacttccg
    96781 cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct tctccgtact
    96841 ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtgc tggcgatcgg
    96901 tctgctggtg gatgacgcca tcgtggtggt ggaaaacgtc gaacgtatta tgagtgagga
    96961 aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg gggcactggt
    97021 cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg gcggcaccac
    97081 cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc tgtcagtact
    97141 ggtagcgatg ctcctcactc cggctctgtg tgccacgcta cttaagccac tgaaaaaagg
    97201 tgagcatcac gggcaaaaag gtttttttgc ctggtttaac cagatgttta accgcaacgc
    97261 cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt agcctgcgct ggattgtgat
    97321 ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga cgtcgttctt
    97381 accgctggaa gaccggggca tgtttactac ctcggtacaa ttgcccagcg gttcaaccca
    97441 acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc atgaaaaaga
    97501 caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg ggcaaaacgt
    97561 ggcgagaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga ccggcacctc
    97621 gtttgccatt atcgagcgtg caacgaaggc gtttaaccaa attaaagaag ctcgcgttat
    97681 cgccagcagc ccaccagcaa ttagcggtct tggtagttct gcaggttttg atatggagtt
    97741 gcaggaccac gctggagcgg gtcacgatgc gctgatggca gcacgtaatc agttgctggc
    97801 gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg atgacagtcc
    97861 gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg atatcgacga
    97921 tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact ttatggatcg
    97981 cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc tgccagatga
    98041 catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct ctgctttcgc
    98101 gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt attctgcggt
    98161 tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg atattatgga
    98221 atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga tgtcgtatca
    98281 ggagcggctt tccggcgcgc aggctccggc gctgtacgcc atttccttgc tggtggtatt
    98341 cctgtgtctg gcggcattgt atgaaagctg gtcggtaccg ttctcggtaa tgctggtcgt
    98401 gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg aaaacgacgt
    98461 ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg ccatcctgat
    98521 cgtcgagttt gctaacgaga tgaaccaaaa aggccatgac ctgtttgaag cgacgctcca
    98581 cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta tcttcggcgt
    98641 attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg tgggtactgg
    98701 cgtaatgggc gggatgattt cagctactat tctggctatt tacttcgtgc cgctgttctt
    98761 tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg gaataagcaa taaaaaaggc
    98821 gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca gcgggttctt
    98881 gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa agtctttcca
    98941 gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta ttctacggaa
    99001 acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct ccgggggaca
    99061 aaccttatgt aaatactatg gtcctgcggt gataatttgt atgtgataca cagcaacatt
    99121 tcgagatatt catacggcat ctaatactga tttaattctg gttaaaatac agacagataa
    99181 caagatgaat attcttaatg tttacgttaa aaatgtttaa tattatttaa tagttgttaa
    99241 tttgaatact tcgataatgt tatattttct gataatcatt tgcaggcaaa atgttttcac
    99301 ccttaaatga gtatttattc tcataaatcg aaaaaggatt cattatggtt acactttacg
    99361 gcatcaaaaa ttgtgacacc attaaaaagg ctcgccgttg gctggaagcc aataacatcg
    99421 actatcgttt tcatgattac cgcgtcgatg ggctggacag cgaattattg aacggtttta
    99481 tcaacgaatt aggctgggaa gcgttactca acacccgtgg tacaacctgg cgtaaactgg
    99541 acgaaactac ccgcaataaa atcaccgatg cggcctctgc ggcggcatta atgactgaaa
    99601 tgcctgcaat tatcaaacgt ccattgctct gcgcgcctgg taagcctatg ctgctgggtt
    99661 tcagtgattc cagttatcag caatttttcc atgaggtgta gtctatgtcg tgcccggtta
    99721 ttgagctgac acaacagctt attcgccgcc cttccctgag tcctgatgac gcaggatgtc
    99781 aggctttgtt gattgaacgt ttgcaggcga tcggttttac cgttgaacgc atggattttg
    99841 ccgatacgca gaatttttgg gcatggcgtg ggcaaggtga aacgttagcc tttgccgggc
    99901 ataccgacgt ggtgccgcct ggcgacgccg atcgttggat caatccgccg tttgaaccca
    99961 ccattcgtga cggcatgtta ttcgggcgcg gtgcggcaga tatgaaaggc tcgctggcgg
   100021 cgatggtggt agcagcagaa cgttttgtcg cacaacatcc caaccatacg gggcgactgg
   100081 catttctgat cacttctgat gaagaagcca gtgcccacaa cggtacggta aaagtcgtcg
   100141 aggcgttaat ggcacgtaat gagcgtctcg attactgcct ggtcggcgaa ccgtcgagta
   100201 tcgaagtggt aggtgatgtg gtgaaaaatg gtcgtcgcgg atcgttaacc tgcaacctca
   100261 ccattcatgg cgttcagggg catgttgcct acccacatct ggctgacaat ccggtacatc
   100321 gcgcagcacc tttccttaat gaattagtgg ctattgagtg ggatcagggc aatgaattct
   100381 tcccggcgac cagtatgcag attgccaata ttcaggcggg aacgggcagt aacaatgtta
   100441 ttccgggtga actgtttgtg cagtttaact tccgcttcag caccgaactg actgatgaga
   100501 tgatcaaagc gcaggtactt gccctgcttg aaaaacatca actgcgctat acggtggatt
   100561 ggtggctttc cgggcagcca tttttgaccg cgcgcggtaa actggtggat gcggtcgtta
   100621 acgcggttga gcactataat gaaattaaac cgcagctact gaccacaggc ggaacgtccg
   100681 acgggcgctt tattgctcgc atgggggcgc aggtggtgga actcgggccg gtcaatgcca
   100741 ctattcataa aattaatgaa tgtgtgaacg ctgccgacct gcagctactt gcccgtatgt
   100801 atcaacgtat catggaacag ctcgtcgcgt gatgagtggt tctgcaagag gaaataagca
   100861 tggactggct ggctaaatat tggtggattc tggtgattgt ctttttggta ggcgtcctgc
   100921 tgaacgtgat taaagatctc aagcgcgtcg accataagaa atttctcgcc aacaagccgg
   100981 agcttccccc gcatcgtgat ttcaacgata agtgggacga tgacgacgac tggccgaaaa
   101041 aggatcaacc gaagaagtaa gctgagtgct caaccgtagg ccggataagg cgttaacgcc
   101101 gcatccggca atggtgaacg atccctgatg cgacgctcac gcgtcttatc aggccaggta
   101161 atctgtcttt tacatcattt caatcacatc atcatcgcca ggcttaccgc cacttagcgc
   101221 ttggtcgaaa taatgtttcg gaatggtata acgcaagtga tccagggcaa attgcatact
   101281 gcgattgtcg atcgcatggc ctaaatcctc cacgatatcc agcgtcacgt caccaccggc
   101341 actgattaac gcttcctgcg ccgcaactgc atgcgccaga tcgataaccg gatcttcacc
   101401 accatgaatc aagtgaattg tcgtggctgt agacgcggtc tccggcaggc tcgcatagcg
   101461 tccgttaaag gcaatgacgc gcgaagcaag gcccggttcg gctttaatgc tctccagcgc
   101521 cataatcgcg ccttgtgaga aaccaattag cgccgtggcg ttagccccta ctccgctctg
   101581 tttctgccag tagcgtaccg tttcaataaa cgtcggcatg atggcatcca cacgcgcctg
   101641 gcgattatct tccgtaatac cctgcactga aaaccactga cgccccgccg gattaccgct
   101701 cggctccgcg ccaccgacac tcaccaccag cgcatcagga aacagcggtg caaaccagct
   101761 gcctatttcc cccattgcca ccgggttatc cccgacaccg tggaaaagca gtaacaactg
   101821 ttgtgcaggt ttatccgggc tttgaacaac aaaatggtca tgtttcatgg cgaactcctt
   101881 aactgatgtc attaatttta cgccgctgac tcacaatgac catgggagaa aattgaagga
   101941 gtcagtgaaa aaattgccat tgcgttatgc gatcgcgcag acgctcagtg cgaacctcat
   102001 ttagtgcgaa cagcgcctgc gcggcctctt cccgctggcg aaccagtaac atcttgcggc
   102061 ctgaaagttt aagtgtggta cataactgcg cgtcactggc gtttttctgt aaacgcccac
   102121 gcagcgccgg taatgccagt tcactggttt gtagcagacg caataagcaa cctaacgatg
   102181 ttaatagcgg acgatgagcg aaagcaaaac cggccagttc aagccagtcg tcgtcagaaa
   102241 ggatggcatc gtttagtgga tcaacaggaa gcatttcgcc attccactgc gcgagagctt
   102301 gcgcatcgcg acgtaaacgg taatgctcac gttcagccag ctgtttaccc gcatcactca
   102361 tcggtaacag cgccatcgcc gtatagcaac cgctgctggc ttcccgatga ttacccatcc
   102421 gcaccagcac aaaaccgcag cgttgccaga aacgccataa ctccccagtg taaccaaaac
   102481 tcaccgaaag atagtcgagg tcatgcgtat attgcaaagc tccagcaata agttgtcgcc
   102541 ctgtgccttc ccgctgacga gccggatgaa ctgctatccg gctaacccgc cttccgcgca
   102601 atgtcgccgc cagtggattg ctgccgtgcg ccgccagcga ctgggccacc agattacccc
   102661 gcgggcgacg aaaacctgcc cataccgcct gactgagttc ttgagataat ccgccctcat
   102721 ccaccagcca caacgccccg gcaatctcgt tttcgccagc cgcctgtaaa aaatgttgcc
   102781 ctggtgcatc catcatccgg cgtaaatcca gcggcgaggt ccggtagtgc gcaccagaca
   102841 ataactgata aacctttaac ggcgtttctg gctcgcttcg ccataacgtt tgttcaaatg
   102901 cggaaatgac gatattgcct tgtggtgtat gggtgaagtt ttcatcgtta aaaaccagtg
   102961 cctcactaac cattttttcc agcgggcatc cctgtgccca gcggatcggc tgttgcagtt
   103021 caaaacggtg taaatgcgga aagcgagcgc aaaatttcag caaaaaacca cgtccggtac
   103081 cttcgtagcc ctgcaccgta gtggttaaca acgttcgagg aaaacgcgat accagttgat
   103141 gcaacaacgg cgcaggtatg gctgcggctt cgtcgaccac cagccagtcg gcttgctcat
   103201 cgctggctaa caaggcatcc ggtgcaataa agcgaaactt ctcgcccgca aattgtgcca
   103261 gtacatccgt tgccgctttt gcgggcgcgg tgacaatcgc actgcccgca atacgagaaa
   103321 tgagttgccc tgccaacgcc gacttaccgc gcccacgcgc agccgttacc gctgccacgc
   103381 ccggcggcat ggtcagtagc tgctgtaaga gttgctgttg ttctggttgt ggtgcgccgg
   103441 tcgcggggtg ccagtcagta cggggagtaa aatgcgccaa cgagaacggc tggttttgcc
   103501 gccagaggat agcgtcgtta tccgccgtaa gtacgcgttt gagatgctgg acaaaatgcg
   103561 gcgtcgcaat agggtcaggg caatcactcc agcgcagcga gtcggcatca ggttggtttt
   103621 cccactcttc ccatacaggg agtaacaaaa ccagccagct tcccgctttc aacgttccgc
   103681 taagtgctgc aaaggcagcg gcatcaaagc cgtggcgggc gtcgaatacc gcatgccgga
   103741 actcgcgccc aagtaaagtt tgtagcgccg agggtgaaca gtggttttca gcatctagct
   103801 gcggcgaaat ccacagccag tcgccaggta aggcatcacg caacttaaga gcatgatcaa
   103861 aacaccaacc ctcttccccg ctcaacacca gcaagcggca gatcccttca cgtttcattt
   103921 gcgctgttaa tgtgtaaagc gcagtcagtt cagccatccc tgccccggaa attaaatgct
   103981 tttaccaaaa gtattgcatt gtgccggatc gccgctgtcg aaaccacgtt taaaccagct
   104041 gtagcgttgc tgagaagtgc catgtgtgaa actgtctggt actactcgcc cctgactttg
   104101 ctgttgtaaa cggtcatcgc cgatggcctg cgccgcattc agcgcctctt ccagatcgcc
   104161 agtttccaga acgccttgct gctgcatact atgcccccag acaccggcaa aacagtcggc
   104221 ctggagttcc atacgcacag ataagcggtt tacttccgcc tgcgtcgcgt tttgttgcag
   104281 ttgacgaact ttcggctcga tgcctaacag tttctgcaca tgatgaccga cttcatgggc
   104341 gataacgtac ccctgggcaa aatcgccatc cgcgccaagt ttgtctttca tgtcatcata
   104401 gaaggagaga tcgatataaa ctgtgccatc cgccgggcaa tagaacggcc ccataatgga
   104461 ctggcccgcc ccgcagccgg tgcgcgtcat tccacggtac atgaccagct tcggttgctg
   104521 ataggtctta cccatctttt cgaactgttg tccccaggtg tcttccgtgg ttgcctgaat
   104581 caccgaggtg aattttgcgg cttcgtcttc attagggcta attgaccgcg ttgattgttg
   104641 ttgagaaacc ggctgcccgg tcatcaaccc ggttaaatca acgccatagt agcctgcaac
   104701 cagcacgact atcagtaaaa tcagcccgcc tttaccgctc ggcagacgga aaccgggacc
   104761 gcccattgac ggaccgccag agctgttgcg cctgtcttca acattgtcac tttcacgtcg
   104821 cccttgccaa cgcatagata cctcaacaat atattcatta tagaaatgat cgtaggtggt
   104881 tcagaggaag attaccacag gaaagcggga tgaaatggcg gaggaatgag cgttcaatgc
   104941 atatattccg agagcggttg tcttgccgtg ccagctgcac ggcaagatga tgaacagaaa
   105001 aatcagtcca gctgtacacc caggcggcgg gcgacggctt cataggcttc gatcaggcca
   105061 ccgaggctct ggcggaaacg gtctttgtcc attttctcca gtgtttcttt gtcccacagg
   105121 cggctgccgt ccggggagaa ctcatcgccc agtaccactt cacctttgta cagaccaaac
   105181 tccagcttga agtcgaccag aatcagacca gcatcatcga acagtttttt cagcacgtcg
   105241 ttcgctttgt aggtcagctc tttcatacgc gccaggttct ctttgctcac ccagccaaag
   105301 gtttcgcagt aagattcgtt gaccatcgga tcgtgcatgg catcgttttt caggaacaga
   105361 tcgaacagcg gcgggttcag ctcaatacct tcttcgattc caagacgttt cactagagag
   105421 ccagcagcac ggttacgcac gacacactca accggcacca tatccagctt tttcaccaga
   105481 cattcggtat cggagagcag acgctccatt tgagtcggga tacccgcttc agccagtttg
   105541 ctcataatga agtagttgaa cttgttgttc accatacctt tgcgatcaaa ctgctcaatg
   105601 cgcgcgccat cccctgctga cgtatcattg cggaattcga gcaccaacag gtccgggttt
   105661 tccgtgctgt atacggtttt cgctttacca cgatacaact cagcttgctt ttgcatcttt
   105721 atcactcctg ggtgtgaatt aacgttttaa aatcttttgc tgtctggtgt gccggatgtt
   105781 ttgtcggatg cggcgtgaac gccttatccg aactacgtcc gacaaacaaa tttcgtgcga
   105841 attaccgcta aaatcgcgtt ttcctgccga cgcacacgtt tgcgtatcat atcagaaaaa
   105901 agggccggat gattccagcc ctgtattttt acttgctaaa cgcagcctgg aagacagcta
   105961 ccagcgcgtc gttctgactc tgagtcagag tatgaccttt cggatcgatg aactgtaggc
   106021 tgctgcggtt atctaaatcg ccaacctgca gtttatagtc accggatgcc aggcctggat
   106081 cgctcgcgcc cagttcctgc cagtcgctgt cagacagcgg cttataagtt acggccatgt
   106141 tgccctgcga acgggtgctg tcggtcactt tcatgcccac tttttccagc gccgctggca
   106201 gacgttgcca aaccacattg aacggcccgc gtacgaccag cattggtaaa ccggtgtcat
   106261 cagctgcact ttgtacgtcc atagtggtgg aggcacgatt ttgcgcagcg ttcgcggcgt
   106321 cagtggcaga tttatccaga ccggcggaaa taacgttcat catctccgtg ctgtaacgct
   106381 gcatggaagc cgcgtctgca accggtttgc ccgcctgttc caggttcagc agtttaaccg
   106441 taaccgcctg ctgataaccc tgcggcttaa cagagatttg ataacgacca cgatactgct
   106501 cgtcttcgtc cagacggttc cattgtaccc aatcggtggt cagtgtctga ccagcatcat
   106561 cacgttgggt gatggtgtag tttttcgcct gcagcacgct aaccacctgc ggccacagag
   106621 tattgccacg accattttcc accagcaatg aagcggtatc gcccgtgaac tgggtacgcg
   106681 cgccagaaac cagtgccaac ggctgggctg gtggacgaat gtccagcgcc ttaccgacag
   106741 caccactacc gttggtcacc gggattgcat aatcaccgga ggtcaccggc aaaatcattc
   106801 cagccggggc atgaagctcc gcaagcggtg ccgcttccag gtaggcttca tcaccactga
   106861 cctgacgctt atagcgtgag tcagaactac aggcagcgag taataaaaca agcgaaacac
   106921 ccgcaacctt tgccaggcgc gacttttgaa cagagtaagc catcaaatct ccctaaactt
   106981 tacagcaaac cggcatgctt aagcgccgct ctgaccgtct cacgaccact gtcggtgatt
   107041 ggtgtcattg gcaggcgcag cgtatcggtc gccacaagac ccagttcctt acatgcccat
   107101 ttcaccggga ttggattggg ttcgacaaat agtttgttgt gtaatggcat cagacgctga
   107161 ttaataacgc gtgcctcggc aaaatgccct tctgctgcca gtttgcacat ctgggccata
   107221 tcacgcgctg cgacgttagc cgtaacggaa ataactccat gaccgcccaa ttgcatgaag
   107281 tccagcgcgc tcgcatcatc gccgctcagc agaacaaaat catctgaaac cagctctttg
   107341 atctggttta cacgcgttaa gttccctgtt gcctctttga ttccgataat attttttact
   107401 ttcgccagac ggcccaccgt ttccgggagc agatcgcagc cagtacggga cggcacatta
   107461 tacagaattt gcggcaggtc agtatgctca gcgatggctt tgaaatgctg atacaaacct
   107521 tcttgcgacg gacgattgta gtaaggggtt accgtcaggc agccgacgat accactgtca
   107581 ttgaagcgct gcgtcaggct aatggcttcc gcagtagcgt tagcgccggt cccggcaatt
   107641 accggaatgc gcccatcagc cagatccagc gtcatcatca ccacatcagc atgttcgtca
   107701 tgatttaagg tagcggactc gccagtggtg ccaacagaaa cgatcgccga agtaccgctg
   107761 gcgacatgat aatcaatcag ttttttcaag ctagcccgac agacattacc tttttcatcc
   107821 atcggagtaa caatcgcgac aatacttccc gtgaacatgg gccatcctct gtgcaaacaa
   107881 gtgtctcaat ggtacgtttg gtatggcatt aaaagcaagc agacagaacc gttctgattg
   107941 ttgtatgcat gattttttta tgctttcctt aagaacaact caccccttaa aggaataacc
   108001 agtttgacac tgtcatcgca acattatctg gtgatcactg cgttgggtgc cgatcgccct
   108061 ggaattgtga acaccatcac ccgtcatgtc agtagttgcg gctgtaatat tgaagacagt
   108121 cgcctggcga tgctgggaga agagttcacg tttatcatgc tgctttccgg ttcatggaat
   108181 gccatcactc tgattgaatc aacgttaccg ttgaaaggtg ccgaactgga tcttttaatc
   108241 gtgatgaagc gcacgacggc gcgtccgcgt ccgccaatgc cagcatctgt ctgggttcag
   108301 gtcgatgttg cagactcccc gcatttaatt gaacgcttca cagcactttt cgacgcgcat
   108361 catatgaaca ttgcggagct ggtgtcgcgc acacaacctg ctgaaaatga acgggctgcg
   108421 cagttgcata ttcagataac cgcccacagc cccgcatctg cggacgcagc aaatattgag
   108481 caagcgttca aagccctatg tacagaactc aatgcacaag gcagtattaa cgtcgtcaat
   108541 tattcccaac atgatgaaca ggatggagtt aagtaatgaa tccactgaaa gccggtgata
   108601 tcgcaccgaa atttagcttg ccggatcaag acggagaaca agttaatttg accgacttcc
   108661 agggacagcg tgttctggtt tatttctacc cgaaagccat gacccccggc tgtaccgtac
   108721 aggcctgcgg cttacgcgat aacatggatg agttgaaaaa agcgggcgtt gatgtgctgg
   108781 gtatcagcac cgataaaccc gaaaaactct cccgttttgc ggaaaaagag ctgcttaact
   108841 ttacgctcct gtctgatgag gaccaccagg tgtgcgaaca attcggcgtc tggggtgaaa
   108901 agtccttcat gggcaaaacc tacgatggta ttcatcgcat cagcttcctg attgacgctg
   108961 atggcaaaat cgaacatgtc tttgacgatt tcaaaaccag caatcaccac gacgttgtgc
   109021 tgaactggct gaaagaacac gcctgattac tttgctccat tccgtgctgg ctgcgcttgc
   109081 ggccagcata cctcacttct cgtgatcaag atcacattct cgctttcccc tgcgacacgg
   109141 gtgtcgaatc cattttttgc tgaacgttaa tgaccatcat ttttgtaccg ttcagaatcc
   109201 agttaataca taacttattg aatagattga gttaatcaga atggcatcct ttatacaata
   109261 tgaaatgcaa tgtttcatat cattttcaag gagccgacat gaaccgcttt gtggtggccg
   109321 aaccactgtg gtgtacagga tgtaatacct gtctcgctgc ctgttcggac gtgcataaaa
   109381 cgcaaggttt acagcaacac ccgcgcctgg ccctggcgaa gacgtcaaca atcactgccc
   109441 ctgtcgtgtg tcatcactgt gaggaagccc cttgcctgca ggtctgcccg gtcaatgcca
   109501 tctctcagag ggatgatgcg atccaactca acgaaagcct ctgtattggc tgcaagcttt
   109561 gcgccgtggt ctgcccattt ggcgcaatca gcgcttcagg aagccgtccg gtgaatgccc
   109621 atgcgcaata tgtttttcag gctgaaggct cactcaaaga cggcgaagaa aacgtgccgc
   109681 cccaacatgc gttgctgcgc tgggaaccgg gtgtccagac cgtcgcggtg aaatgcgacc
   109741 tgtgtgattt cttgccagaa ggtccggcct gcgttcgcgc ttgcccgaat caggcgttac
   109801 ggctgatcac cgacgatagc ctgcaacgtc agatgaaaga aaaacagcgc cttgccgcaa
   109861 gctggtttgc caatggcggg gagtctcccc tttccctcac tcaggagcaa cactaatgga
   109921 tgccctgcaa ttattaacct ggtcgctgat cctctatctg tttgccagtc tggcttcgct
   109981 gtttttactc ggtctggaca gactggctat taagctttcc ggcatcacat cgctggtggg
   110041 cggcgtgatt ggcatcatca gcggaattac gcaattacat gctggtgtaa ctttagtcgc
   110101 ccattttgcc accccttttg actttgccga tttaaccctg cgaatggata gcctctcggc
   110161 atttatggtg ctagttatct ccttgctggt ggtggtttgt tcgctctatt cattgactta
   110221 tatgcgcgaa tacgagggca aaggcgcggc ggcgatgggc ttctttatga atcttttcat
   110281 cgcatcgatg gttgccctgc tggtgatgga caacgctttt tggttcatcg tgctgtttga
   110341 aatgatgtcg ctgtcttcct ggtttctggt cattgccagg caggataaaa cgtcgatcaa
   110401 cgctggcatg ctctactttt ttatcgccca cgccggatcg gtgctgatta tgatcgcctt
   110461 cttgctgatg gggcgcgaaa gcggcagcct cgattttgcc agtttcgcac gctttcactt
   110521 tctccggggc tggcgtcggc ggtgtttctg ctggcctttt tcggttttgg cgcgaaagcc
   110581 gggatgatgc cgttgcacag ctggttgccg cgcgctcacc ctgccgcacc atcgcacgct
   110641 tcggcgttga tgtctggcgt aatggtcaaa atcggtattt tcggcatcct gaaagtagcg
   110701 atggatctgc tggcgcaaac gggtttgcct ctgtggtggg gcattctggt gatggcgatc
   110761 ggcgcaatct ccgcgctcct gggcgtgcta tatgcgctgg cggaacagga tatcaaacgg
   110821 ctgctggcct ggagtaccgt cgaaaacgtc ggcattattt tgctggcagt cggtgtggcg
   110881 atggacggtc tgtcactgca cgacccgctg ctcaccgtgg ttggactgct cggcgcactg
   110941 tttcatctgc tcaaccatgc gctgttcaaa gggctgctat ttctcggcgc ggacgcgatt
   111001 atttcgcgtt tgcataccca cgacatggaa aaaatggggg cactggcgaa acggatgccg
   111061 tggacagccg cagcatgcct gattggttgc ctcgcgatat cagccattcc tccgctgaat
   111121 ggttttatca gcgaatggta cacctggcag tcgctgttct cactaagtcg tgtggaagcc
   111181 gtagcgctac aacttgcggg tcctattgct atggtaatgc tggcagtcac tggtgggctg
   111241 gcagtaatgt gcttcgtcaa aatgtacggt attactttct gtggtgcgcc gcgcagtaca
   111301 cacgctgaag aggcacagga agtgccaaat acgatgatcg tcgccatgct actgctcgcg
   111361 gcactctgcg tattaattgc gcttagtgcc agttggctgg caccgaagat aatgcatatt
   111421 gcccatgcgt ttaccaatac ccctcccgtc actgtcgcca gcggaatagc acttgtaccc
   111481 ggcacgtttc atacacgggt cactccttca ttactgttgc tgttactact ggcgatgcct
   111541 ttgctgccag gcctttactg gctgtggtgt cgttcgcgcc gcgcagcgtt tcgtcgcaca
   111601 ggagatgcct gggcatgcgg ctacggctgg gaaaacgcga tggctccgtc aggcaatggc
   111661 gtgatgcagc cgctgcgtgt ggtcttctct gcgttatttc gtctacgaca acagctcgac
   111721 cctacgctga ggctaaataa aggtcttgcg cacgtcaccg ccagggctca gagcacagaa
   111781 cccttctggg atgagcgggt gatccgcccc atcgtgagcg ccacccaacg gctggtcaaa
   111841 gaaatacagc atctgcaaag cggcgacttt cgtctctatt gcctgtatgt ggtcgccgca
   111901 ctggttgtgc tgctaatcgc tattgccgtc taaggaaatc accatgagac aaactctttg
   111961 cgacggatat ctggtcattt ttgcgttagc acaggccgtg attctgctga tgctaacccc
   112021 actttttacg ggtatttccc ggcagatacg cgcgcgtatg cactcccgcc gcgggccggg
   112081 gatctggcag aattatcgcg atatccacaa actgtttaaa cgccaggaag ttgcgccgac
   112141 atcttcaggt ctgatgttcc gcctgatgcc gtgggtatta atcagcagca tgctggtgct
   112201 ggcgatggcc ttaccactat ttattaccgt ttcccctttt gcgggcggcg gcgatctgat
   112261 cacccttatc tatcttcttg ccctgtttcg ttttttcttt gctctttccg ggctggatac
   112321 cggaagtccg tttgcgggag tcggtgccag tcgcgagttg acgctcggca ttctggtcga
   112381 accaatgctt attctctcac tgctggtatt ggcgctgata gcaggttcca cgcatatcga
   112441 gatgatcagc aatacgctgg ctatgggctg gaactcgccg ctaaccaccg tactggcgtt
   112501 actggcctgt ggttttgcct gcttcattga gatgggaaaa attccctttg atgttgctga
   112561 agcagaacag gaattacagg aaggcccgct gaccgaatat tccggtgccg ggctggcgct
   112621 agcgaaatgg gggctggggc tgaaacaggt cgtgatggca gcactgtttg tggccctgtt
   112681 tctgcccttt gggcgcgcgc aagaactttc tctcgcctgc ctgctgactt cacttgtcgt
   112741 tacgctgctc aaggttttgc tgatttttgt actggcctca atcgcagaaa acacgctggc
   112801 acgcgggcgt tttttactca ttcaccatgt gacctggctt ggcttcagcc ttgctgcgct
   112861 tgcatgggtc ttttggttaa ccggtctgta aggagcactg acggaatatg gaaaatcttg
   112921 ctctgacgac gttaatgctg ccttttatcg gcgcactggt cgtttcgttt tcgccacaac
   112981 gtcgggccgc cgaatggggg gttttgttcg ccgcgctgac cacgctgtgc atgttgtcgc
   113041 tgatctccgc gttttatcag gccgataaag ttgccgtcac gttgacgttg gtcaacgtgg
   113101 gggatgtggc attatttggc ctggtcattg atcgcgtgag tacgctgatt ctgtttgtgg
   113161 tggtgtttct cggtttgctg gtcacgatct actccacggg ttatctgacg gataaaaatc
   113221 gcgaacaccc gcataacggc acgaatcgtt attacgcatt tttgctggtg tttatcggcg
   113281 cgatggcggg actggtactc tcctcgacgc tgctcggtca gttgttgttt tttgaaatta
   113341 cgggcggctg ctcctgggcg ttgatcagtt attaccagag cgataaagcg cagcgttcag
   113401 cactaaaagc gttgcttatc acccatatcg gttcgttggg gttgtatctt gccgccgcca
   113461 cgctgttttt gcagaccgga acgtttgtgc ttagcgcgat gagcgagtta catggcgacg
   113521 cacgttatct ggtttatggc ggcatcctgt ttgccgcgtg ggggaaatcg gcccagctac
   113581 cgatgcaagc gtggctaccg gacgcaatgg aagcgccaac accgatcagc gcctatctcc
   113641 acgccgcatc gatggtgaaa gtgggcgttt acatttttgc ccgcgccatt atcgacggcg
   113701 gcaatatccc gcatgtgatt ggcggcgttg gcatggtcat gacactggtc accattcttt
   113761 atggctttct gatgtatttg ccacagcagg atatgaagcg gttgctggcc tggtcgacca
   113821 tcactcaact tggctggatg ttcttcggct tgtcgctctc catcttcggc tcgcggctgg
   113881 cgctggaggg tagcatcgcc tacatcgtca accacgcgtt cgctaaaagc ctgtttttcc
   113941 ttgtagcagg tgcgctgagt tacagctgcg gcacgcgctt gttgccgcgt ctgcgtggcg
   114001 tattgcacac cctgccgttg ccaggcgtgg atttctgcgt ggcagcgctg gcaattaccg
   114061 gtgtgccgcc gttcaacggc ttcttcagta aattcccgct gtttgccgcc ggttttgcgt
   114121 tgtcagtgga gtactggatc ctgctgcccg ccatgattct gctgatgatt gaatcggtcg
   114181 ccagtttcgc ctggtttatt cgctggtttg gtcgcgtcgt gccgggcaaa ccgagcgagg
   114241 ccgtcgccga tgccgcaccg ctgccagggt caatgcgcct ggtgttgatt gtactgattg
   114301 tgatgtcgct gatttccagc gtaatcgccg cgacctggtt gcagtaagga gatgatgaat
   114361 gaccggttct atgatcgtaa ataatctggc gggactgatg atgctgacat cgctgtttgt
   114421 gattagcgtc aaaagctatc gcctgtcatg cggattttac gcctgccagt cgctggtgct
   114481 ggtgtctatt ttcgccactc tctcgtgcct gttcgccgca gagcaactgc tgatctggtc
   114541 cgccagcgcc tttatcacca aagtgctgct ggtaccgtta atcatgactt acgctgcacg
   114601 aaatattccc cagaacatcc cggaaaaagc gttattcggt ccggcaatga tggcactgct
   114661 cgcggcgtta attgtcctgc tttgcgcatt tgtcgttcag cccgtgaagc taccgatggc
   114721 taccgggctg aaaccggcgc tggcggtagc gttaggtcat tttctgcttg gcctgctgtg
   114781 cattgtcagc caacgcaata tcctgcggca aatttttggt tactgcctga tggaaaacgg
   114841 ctcccatctg gtgctggcgc ttcttgcctg gcgagcaccg gaactggtgg aaataggtat
   114901 cgctaccgac gccatcttcg ccgtcattgt gatggtgtta ctggcaagaa aaatatggcg
   114961 tacccacggc acgctggacg tgaacaactt gaccgcgctg aagggataat gagatgagtt
   115021 attctgtgat gttcgcttta ctcctgctca cgccgctgct tttttcgctg ctctgttttg
   115081 cctgccggaa acgggggctc tctgcgactt gtacggtgac tgtattacat agcttaggga
   115141 tcacactgct gctgattcta gcactctggg tggtccaaac tgccgctgat gcaggagaaa
   115201 tattcgctgc gggactgtgg cttcatattg atggtctggg cggtttgttc ctcgccattc
   115261 ttggtgtgat tggctttctc accggtgttt actcgattgg ctacatgcgt catgaagtgg
   115321 cacacggcga gctttcaccc gttacactgt gcgattacta cggtttcttc catctgtttt
   115381 tgttcaccat gctgctggtt gttaccagca ataacctgat tgtgatgtgg gcggcgatcg
   115441 aagccaccac cttaagctcg gcgtttctgg taggcattta cggtcagcgt tcatcgctgg
   115501 aatcagcatg gaagtacatc attatttgta ctgttggtgt cgcttttggt ctgttcggta
   115561 ccgtgctggt atacgccaac gccgccagcg ttatgccgca ggcagaaatg gcgatattct
   115621 ggagcgaggt tcttaagcaa tcgtccttgc ttgacccaac attaatgctg ttggcctttg
   115681 tgtttgtgct aattggcttt ggtaccaaaa ccgggctatt tcccatgcac gcctggctgc
   115741 cggatgctca cagtgaagcg ccgagtccgg tcagcgccct gctctccgcc gtattgctga
   115801 actgcgcgct gttggtgctg attcgctatt acatcattat ttgccaggcc atcggcagcg
   115861 atttccccaa ccggttgttg ctcatcttcg gcatgttgtc ggttgccgtg gcggcatttt
   115921 tcattctggt acagcgggac attaagcgtc tgctggcgta ctccagcgtg gagaacatgg
   115981 ggctggtcgc ggtggcgcta ggcattggcg ggccgctggg aatttttgcc gcgctgctgc
   116041 acaccttaaa ccacagtctg gcaaaaacgc tgctgttctg cggttccggc aatgtactgc
   116101 tcaagtacgg cacgcgcgat ctcaacgtcg tctgtgggat gctcaaaatc atgccattta
   116161 ccgccgtgct gtttggtggc ggtgcgctgg cgctggcgct ggcgctggca gggatgccgc
   116221 ccttcaacat ttttcttagc gaatttatga ccgttaccgc aggactggca cgtaatcacc
   116281 tgctgattat cgtcctgctg ttattgctgt taacgctggt gctggcgggc ctggtacgga
   116341 tggctgcgcg ggtgttaatg gcgaaaccgc cgcaggccgt taaccggggt gatctcggct
   116401 ggttgaccac ctcgccaatg gtgattctgc tggtcatgat gctggcgatg ggaacgcata
   116461 ttccacaacc tgtcatcagg atcctggcgg gcgcttccac tatagtcctc tcagggacgc
   116521 acgacctgcc tgcacaacgt agcacctggc atgatttttt gccttcaggc accgcatctg
   116581 tttcggagaa acacagtgaa cgttaattca tcgtcaaatc gtggcgaagc gattctcgcc
   116641 gccctgaaaa cgcagttccc cggcgcggtg ctggatgaag agcgacaaac gcctgaacag
   116701 gtcaccatta cggtgaaaat caatctgctg cctgacgttg tacattatct ttattatcaa
   116761 catgatggct ggcttccagt cctgtttggc aacgacgagc ggacacttaa cggtcattac
   116821 gcggtttatt atgccctttc aatggaaggg gccgaaaaat gctggatcgt ggtgaaggca
   116881 ctggtcgatg ccgacagtcg ggagtttccg tcagtcacac cgcgcgtccc tgccgcggtc
   116941 tggggcgagc gagaaattcg cgatatgtac gggctgattc cggttggcct gccggatcag
   117001 cgtcgcctgg tgttgcccga tgactggccg gaagatatgc atccgctgcg caaagatgcg
   117061 atggattatc gactgcgccc tgaaccgacg acagattccg aaacgtatgc gtttatcaat
   117121 gagggcaaca gcgatgcgcg ggtgatccct gtcggcccgc tgcatatcac ctcctatgaa
   117181 ccgggtcact tccgcttgtt tgtggatggc gagcaaattg tcgatgctga ttaccgcctg
   117241 ttttatgtcc atcgcggcat ggagaaactg gcagaaacgc ggatgggcta caacgaagtg
   117301 accttcttat cggaccgcgt gtgtgggatt tgcggttttg cccacagtgt ggcctatacc
   117361 aactcggttg aaaatgcact ggggattgag gtgccgcaac gagcacatac tattcgctcg
   117421 attctgctgg aagtcgagcg gctacacagt catttgctca accttggcct ctcctgccat
   117481 tttgttggtt ttgataccgg ctttatgcaa tttttccgcg tgcgggaaaa gtcgatgacg
   117541 atggcggaat tgctgaccgg gtcgcgtaaa acctacggtc tgaatctgat tggtggtgtt
   117601 cgccgcgata ttctcaaaga gcaacgtctg caaacgctga aactggtgcg cgagatgcgc
   117661 gccgacgtgt cggagctggt agagatgctg cttgctacgc cgaatatgga acaacgcact
   117721 cagggcattg gcattctcga ccgacaaatc gcccgtgatt atagccctgt agggccgctg
   117781 atccgcgcag tggttttgcc cgtgatttgc gctttgatca cccctacgcc gactacggca
   117841 atattccaaa aacactgttt acctttaccg gcggcgatgt tttctcccgc gtgatggtcc
   117901 gtgtcaaaga gacgtttgat tcgctggcaa tgctggaatt tgccctcgac aacatgccgg
   117961 ataccccact gctgaccgaa ggctttagct ataaacctca cgcattcgcg ctgggctttg
   118021 ttgaagcgcc acgcggtgaa gacgtgcact ggagcatgct cggtgataac caaaaattgt
   118081 tccgctggcg ctgtcgtgcc gccacctacg ccaactggcc ggtgttgcgt tacatgctgc
   118141 gcggcaatac cgtttctgac gcaccgctga ttatcggtag ccttgatccc tgctactcct
   118201 gtaccgaccg tgtgacgctg gtagatgtgc gcaagcgcca gtcaaaaacc gtgctgtata
   118261 aagagatcga acgctacggc attgatcgta accgttcgcc gctgaagtaa ggacagaaga
   118321 tgctgaagtt actgaaaact attatgcgcg ccggaaccgc gacggtgaaa tatcccttcg
   118381 cgccactgga ggtcagccca ggctttcgcg ggaaaccgga cctgatgccc agccaatgta
   118441 ttgcctgcgg tgcctgcgcc tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg
   118501 accagcaaaa ttcgcgcacc tggcagctct atctggggcg ttgtatttac tgcggacgtt
   118561 gtgaagaagt gtgcccgacc agagccatcc agcttaccaa taactttgaa ctgaccgtca
   118621 cccataaagc cgatctctat acccacgcga cgttccatct acaacgttgc agccgttgcg
   118681 aacgcccgtt tgccccgcaa aaaaccgtcg cactggctgc tgaattgttg gcacagcaac
   118741 aaaatgctcc acaaaaccgc gaaatgttat gggcgcaagc gagcgtctgc ccggagtgca
   118801 aacaacgcgc aacgctgatc aacgacgata cagatgtacc gctggtggct aaggagcagc
   118861 tatgagtcca gtgcttacgc aatatgtcag ccagcccatc acgctggacg agcaaacgca
   118921 aaagatgaag cggcatttgc tacaggatat ccgtcgctcg gcttacgttt atcgcatcga
   118981 ttgcggcggc tgcaacgcct gtgaaatcga aatttttgct gccatcaccc ccgtattcga
   119041 cgcagaacgt tttggcatta aggttgtttc atcaccgcgt cacgccgata ttttgttatt
   119101 tactggcgca gtcacccggg cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc
   119161 cgatcataaa atctgtgttt cctacggcgc gtgcggtgtc ggcggcggta ttttccacga
   119221 tctctacagc gtctggggcg gcagcgacac cattgtcccc attgatgttt ggatccccgg
   119281 ctgcccgcca acaccggccg ccaccattca cggtttcgcc gtggcgctcg gtttgctgca
   119341 acagaagatt cacgcggtgg attatcgcga tcccaccggg gtgactatgc aaccgttgtg
   119401 gccgcagatc ccgccatcac agcgtatcgc cattgagcga gaagcgcggc ggctggcggg
   119461 ctatcgtcag gggcgagaaa tttgcgatcg gctcctgcgc catttaagcg acgatcctac
   119521 aggaaatcgg gttaacacct ggttgcgcga tgccgacgat ccacgtctca atagtatcgt
   119581 tcagcaactc tttcgcgtac tccgggggtt acatgactga agagtgcggg gaaattgttt
   119641 tctggacgct gcgaaaaaag tttgtcgcca gtagcgacga gatgccggaa cacagctctc
   119701 aggtaatgta ttactcgctg gctatcggtc atcacgttgg cgtgattgat tgtctgaatg
   119761 tcgccttccg ctgcccactg acggaatacg aagattggct tgcactggtc gaagaggagc
   119821 aagcccgacg taagatgctg ggggtgatga cttttggtga gattgttatt gacgccagcc
   119881 acaccgccct gttgacccgg gcattcgcgc cactggcaga tgacgcgacg tctgtgtggc
   119941 aagcgcgtag cattcaattc attcatctgt tggatgaaat tgtgctggaa ccggctatct
   120001 atctgatggc cagaaaaatt gcgtgagaag gatttctcat taataaggac tgttgatggc
   120061 tatgtcagac gaagcgatgt ttgccccgcc gcaaggaata acaattgaag cggtaaacgg
   120121 aatgctcgcg gagcggttag cgcagaaaca cggcaaggcg tctttattac gcgccttcat
   120181 cccgctgccg ccgccgttca gcccggtaca acttattgaa ctgcatgttc tcaaaagcaa
   120241 cttctattac cgctaccatg atgatggcag cgatgtgacg gcaacaacag agtatcaggg
   120301 cgagatggtc gattattcgc gttacgccgt ccttctcggc agtagtggaa tggcggagct
   120361 acgctttatt cgtacccacg gcagccgttt tactccccag gattgcacac tgtttaactg
   120421 gctggcacgc attatcaccc cggttctgca atcatggctc aatgatgaag cacagcaggt
   120481 ggcgctgcgt ttgctggaga aagatcgcga tcatcatcgg gtactggtgg atatcactaa
   120541 tgcagtgctg tcacatcttg atctcgacga tctgatcgct gacgtcgctc gtgagatcca
   120601 tcattttttc ggtctggctt cagtcagtat ggtactgggc gatcatcgaa agaacgagaa
   120661 gttcagcctg tggtgcagcg atctttctgc ctcacattgt gcgtgtctgc cacacaatat
   120721 gcctggcgac agtgtattgc tgacacaaac gctacaaacc cgacaaccga ccttgacgca
   120781 ccgtgcagat gatctgtttc tctggcaacg cgacccgtta ttactcttac ttgcatctaa
   120841 cggctgcgaa tctgcgctcc ttataccgct tacctttggc aaccatacac cgggtgcatt
   120901 gttgctggcg catacctctt ccactctctt tagtgaggaa aactgccagc tactacaaca
   120961 catagccgat cgcatcgcta ttgccgttgg caatgccgat gcctgccgta gaatgaccga
   121021 tttgcaggaa agtttgcagc aagaaaacca ccagcttagc gagcagctcc tttcgaatct
   121081 gggcgtcggt gacattatct atcaaagcca ggcaatggaa gacctgctcc agcaggtaga
   121141 tattgtggcg aagagcgaca gtacggtgtt gatttgcggt gaaaccggaa ccggcaaaga
   121201 ggtgatcgcc agagcgatcc atcaacttag cccgcgacac gacaagccgc tggtcaaaat
   121261 caactgcgct gccatccccg ccagtcttct ggaaagtgag ttattcggtc atgacaaagg
   121321 ggcgtttact ggtgcgatta atacccatcg tggtcgtttt gaaattgccg atggcggcac
   121381 gttgtttctc gatgaaattg gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt
   121441 attgcaggaa cgggagattg agcgtctcgg cgggagtaga acgatcccgg taaatgtcag
   121501 agtcattgcc gccaccaacc gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag
   121561 cgatctcttt tatcgcctga atgtcttccc actggaattg cctccgctgc gcgaccgtcc
   121621 ggaagatatc cctcttttag caaagcattt cacgcaaaaa atggcgcgcc atatgaatcg
   121681 ttcaattgac gccatcccga ccgaggcgct acgccagttg atgtcgtggg actggccggg
   121741 caacgtgcgc gagctggaaa acgtgattga gcgagcggta ctgctgactc gcggtaacag
   121801 tctgaattta catcttaatg tccgacaaag ccgtttactg ccgacgctaa atgaagattc
   121861 agcgcttcgc agttcaatgg cgtagttgct gcacccgacg acgccagaga atgacgaaga
   121921 agaacgtcag cgcattgttc aggtattgcg agaaaccaat ggcattgttg ccgggccccg
   121981 tggcgcggcg acacgattag ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct
   122041 gggaatctcg gttcgcgagg tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa
   122101 actctctttc gacttgcagt tgagcgccag aaaagcggca atcgctgaac ggattgccgc
   122161 ccataaaatt gcccgcagta aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt
   122221 tatggcgatg tccgctggcg tgtttatggc gatcggattt actttttacc tttccgttat
   122281 cgccgatgcc ccgtcttcat aggcattaac ccatctggtg ggcggccttt gctttacact
   122341 tggctttatt ttgctggcgg tttgcggcac cagcctgttc acctcgtcgg taatgacggt
   122401 aatggcaaaa agtcggggcg ttattagttg gcgaacttgg ctgattaacg cacttctggt
   122461 ggcctgcggt aatctggcag gtattgcctg tttcagtttg ttaatctggt tttccgggct
   122521 ggtgatgagt gaaaacgcga tgtggggagt cgcgatttta cactgcgccg agggcaaaat
   122581 gcatcataca tttactgaat ctgtcagcct cggcattatg tgcaatctga tggtttgcct
   122641 ggcgctgtgg atgagttatt gcggacgttc gttatgcgac aaaatcgtcg ccatgatttt
   122701 gtccatcacc ctgtttgtcg ccagtggctt tgagcactgt atcgccaatt tgtttgtgat
   122761 tccgttcgcc attgccattc gccatttcgc ccctacctcc ttctggcaac tggcgcacag
   122821 tagcgcagac aattttccgg cactgacggt cagccatttt attaccgcca atctgctccc
   122881 ggtgatgctg ggtaatatta tcggcggtgc agtgctggtg agtatttgtt atcgggctat
   122941 ttatttacgt caggaatcct gaaaaatcag cccggcgaaa cagttcgtcg ggctgaagat
   123001 tattcttgcg cgatttgccc atcgggccag gcgtggatca cggctttgat cagcgtcgcc
   123061 aatggaatgg cgaagaatac gccccagaat ccccacaaac caccgaagat caccaccgat
   123121 aaaataatca ccagcggatg caggttaacc gcttcggaga acaacaccgg tactagcagg
   123181 ttgccgtcca gcgcctgaat aatcagatac accgcgaagc agctccagaa ttcagtacct
   123241 gcgccaaact ggaatagcgc cacgccaacc accggaatgg tcaccacaaa tgcgccaatg
   123301 tacggaataa gaaccgagaa gccgaccagc actgccagca gcagcgaata gttcagcccg
   123361 aagagcaaga accccagcca ggtggcgatc cccaccacga tcatctccag cactttgccg
   123421 cggatatagt tggtgatttg ttgattcatc tccttccaca cctgtcctgc cagcccacgg
   123481 ttacgcggca gcacccggcg aacggcgttc agcatctgct ctttgtcttt cagcaggaag
   123541 aagaccatca atggcaccag caccagatag acggctatgg tcagcaaacc gaccagcgag
   123601 gcgagggaaa ttttcaccac cgaatcgccc atggtcagca tccgactgcg catattttcg
   123661 gccattgcat caataatgcc cgcgtccatt aacaccggat agcggcgcgg caacgtggcg
   123721 gcaaagtcag aaagcttatt gagcatcccc ggcatatcgc ggattaagta gatgccctgt
   123781 tgccaggcga taggcagtac cacgaacgcc atcagtagca atataccgac aaaaaccacc
   123841 aatacaatcg acgtcgccca gcggcgggag cagccaatag attgcaggcg cacggttggc
   123901 cattccagca aataagccag cacaatagcc accagcaacg gagcaagcag gccactaaag
   123961 aaaaagataa tgccaaatcc ggcaactaaa ataaccagca aggcaatcgc ttccgggtcg
   124021 ctaaaacggc ggcgatacca ttgcatcaac atttcgagca taaagacctt ccctgaacct
   124081 caagagcggg attgcgatcc gcaattgtat cgaaatgtca caaaaaagac ttcgcttttt
   124141 atgacggatt caggaaactg aaaagtcatt tgagtgggct aatcttcgcc gttacactca
   124201 aaggcggcga ggtgggaacg atatttcaca gtatcggtca aatgactatc ttcagaaata
   124261 caggatagag gttatgttca ggcagttgaa aaaaaacctg gttgcaaccc tcattgctgc
   124321 tatgaccatt ggtcaggtag ccccggcgtt tgccgacagc gcagacacct tgccggatat
   124381 gggaacctcc gcaggaagca cgctttccat tggtcaggaa atgcagatgg gcgactatta
   124441 tgtccgccag ctacgcggca gcgcgccgtt aattaatgac ccgctgttaa cgcaatatat
   124501 taattcgctg gggatgcgtc tggtttcgca tgccaattcg gttaagacac cgtttcattt
   124561 ttttctgatc aacaacgacg aaattaacgc ctttgctttc tttggcggca acgtggtgct
   124621 gcactctgcc ctgttccgtt attccgataa cgaaagtcaa ctggcttcag ttatggcgca
   124681 cgaaatctcc cacgtcaccc aacgtcacct ggcgcgagcg atggaagatc aacaacgcag
   124741 cgcgccgctg acctgggtcg gcgcgttagg ttctatttta ctggcgatgg ccagtccgca
   124801 ggcggggatg gcggcgctga ccggtacact ggcgggaacg cgccagggga tgatcagttt
   124861 cacccagcaa aatgaacagg aagcggaccg cattggtatt caggtgctgc aacgctcggg
   124921 attcgatcca caggcgatgc cgaccttcct ggaaaaattg ctcgatcagg cgcgttactc
   124981 ctcgcgcccg ccggaaattt tattgactca cccgttgccg gaaagtcgtc tggcagatgc
   125041 ccgcaaccgt gctaatcaga tgcgcccgat ggtggtgcag tcgtcggaag atttctatct
   125101 ggcgaaagcg cgcacactgg ggatgtataa ttccggacgt aaccagctca ccagtgattt
   125161 gctggatgaa tgggcgaaag gaaacgttcg tcagcaacga gcggcgcaat atggtcgtgc
   125221 tttacaggcg atggaagcca ataaatacga cgaggcgcgt aaaacgctgc aaccgttact
   125281 ggcggcagaa cctggcaacg catggtatct cgatctggct accgatatcg atcttgggca
   125341 aaacaaagcc aatgaggcaa tcaatcgcct gaaaaatgcc cgcgatttac gcaccaatcc
   125401 ggtgttgcag ctcaacctgg cgaacgctta tctgcaaggc ggtcaaccac aagaagcggc
   125461 caatattctg aatcgctaca cctttaataa taaagatgac agcaacggct gggatttgct
   125521 ggcacaggcg gaagccgcgc taaataaccg cgatcaggag ctggctgcgc gagcagaagg
   125581 ttatgcgctc gccggacgac tcgatcaggc catttcgctg ttgagtagcg ccagttcgca
   125641 ggtgaaatta ggcagcctgc aacaagcgcg ttacgatgcg cgcatcgacc agttgcgcca
   125701 gctgcagaaa cgctttaagc cttataccaa gatgtaattc gcattaaagg aagaacaatg
   125761 accaaacagg taaaaatcta ccataaccca cgctgttcaa agagccggga aacgctgaac
   125821 ctgctgaaag aaaacggcgt ggagccagaa gtggtgctct accttgagac acccgccgat
   125881 gcggcaacgc tgcgcgattt gctgaaaatg ctggggatga atagcgcccg tgaattgatg
   125941 cgccagaaag aggatcttta taaagaacta aacctggctg acagttcgct tagcgaagag
   126001 gcgctgattc aggcaatggt ggataatccg aagctgatgg agcgcccgat tatggtcgcg
   126061 aatggcaaag cgcggattgg tcgtccaccg gagcaggtac tggaaatcgt gggttaagat
   126121 gtgctccatc gctagttgaa gcacattgcc ggatgcgacg cttgccacgt cttatccggc
   126181 ctacgaatac tatggattaa cttcgcgtag gtcaggcgtt cgcgccgcat ccgacaataa
   126241 acaccttatc tacaacttca gaatttcttt cacaaacgga atggtcagct tacgttgcgc
   126301 ggtaatcgac gcacgatcca actgatccaa cgtcataaat agcgtgcgca tttctctgtc
   126361 gagccgcttc agcaagaaac gccccacatc ttccggcagt tcaaaaccac gcaaacgcgc
   126421 gcgtaactgt agcgcctgca acttatcttc atcagaaagt ggctgcaatt tgtagatctg
   126481 cccccagtcg agacgcgacg cgagatccgg taatcccaga ttcaactgcc gcggtggacg
   126541 atcgccggtg atcaacaacc gtgttttgcc cgattccaga attcgattgt agagatcgaa
   126601 aatcgccatc tcccacaact catcgcctgc aatacactca atgttgtcga tacagaccag
   126661 cgacaaatgc tccataccgt cgagcacttc cggaacaaac caggtgcgtt tatccagcgg
   126721 gacatagccc accgcatcgc cacgctgcga caattccgcg caagccgcgt gcagcagatg
   126781 gctgcgcccc gcgccttcgc gtgcccagag atagatgtaa ccgctatgtt cctgacgcag
   126841 cacgttttgc agcgcggcca gtaaagagga gttatccccc ggccagaaac ttgcaaaggt
   126901 ttcgtcgtca ggaagataaa gtggcaaaga gagctgtgcc ggtgtgttca gagatacctc
   126961 aaccaggatt tcacaaaatc gcgagaagtt taccacagaa tcccatgatg tttgaaccgg
   127021 gcagcaacac tgcccggtcg gtacattatt tgtctgttat atccgcgtct tctgcgtcca
   127081 gcaccacttc ttcagggcgc agcacactga tcagtttgaa aatcaaactt aagccgatac
   127141 cgacgatggt cgccagcgcc atacctttta actccgccgc gccgatgttt accttcgcgc
   127201 cactgacgcc gatgatcaaa atcacggaag tcaggatcag gttctgtgct ttgttgtaat
   127261 ccactttcga ttcgatcaaa acacgaatac cggaagcacc aatgacacca taaagcagca
   127321 gcgaaacgcc gcccatcacc ggcaatggga tcatctggat agcggcagcc agtttaccga
   127381 cgcaggaaag caggatagcg aaaatcgccg ccccgccgat aacccaggta ctgtaaacac
   127441 gggtgatcgc catcacgcca atgttttctc cgtaagtggt atttggcgta gagccaaaga
   127501 agccggagat cacggtcgac aggccgttag caaacatcga acggtgcaga cctggatcgc
   127561 gcagcagatc ttttttgacg atattagccg ttactaccag gtgccctacg tgttcggcaa
   127621 taaccactaa cgccgctggc agaatagtca gaatggcaaa ccactcgaag cgcggcgtat
   127681 agagggttgg cagtgcaaac cagtgagcat taataatcgg cgtggtatcg acaattccca
   127741 ttgcgaaaga gagcgcgtac cccaccagca cgccaattaa aatcgggata attgccagga
   127801 aaccacgaaa cagcacggaa cctaaaaccg tgaccgccag ggtggtaata gagatgatga
   127861 tggttttgga gtttggcgtt tgcccttcag ccgggagtaa acccgccata ccggcagcta
   127921 cgcccgccag ctccagaccg atgacggcaa cgattgcgcc cattgccgca ggtggaaaca
   127981 gcacgtccaa ccagccggtc cccgctttct tcacgataaa agaaaccagg cagaacagca
   128041 cgccgcacat aataaagccg cccagcgcga cttcataccc taacggcaac agtaacaata
   128101 ccggtgaaat aaaggcaaag ctggaaccaa gataagccgg aattttccct ttacagatga
   128161 agagatacag cagcgttcca ataccgttaa ataacagtac agtcgccggg ttaatatgaa
   128221 ataagacggg caccaggacg gttgcaccaa acatggcgaa caaatgttgc aaactaagcg
   128281 ggattgtctg taaaagtggc ggtctttcac tcaccccgat agcacggcgc gtcatagtat
   128341 tatcctctgt attatgtgtt ataggcgctt tactcaaaaa aaagccgact cttaaagtcg
   128401 gctttaatta tttttattct ttatttcgta ccaaagattt tgtcaccggc atcgccgagg
   128461 cccggaataa tgtatccgtg ctcgttcagt ccctggtcaa tcgatgcagt atacagttcg
   128521 acgtccgggt gcgctttttc cagcgcagcg ataccttctg gcgcagcgac cagcaccaga
   128581 actttgatgc tgctgcagcc cgcttttttc agcaggtcga tggtcgcgat aacggaacca
   128641 ccggttgcca gcattgggtc aacgatcagc gccatacgct catcgatgtt agaaaccagt
   128701 ttctggaagt acggtaccgg ctccagcgtt tcttcattac ggtacatacc gacaacgctg
   128761 atgcgcgcgc tcggaacgtt ttccagcaca ccgtccatca taccaagacc cgcacgcaga
   128821 attggcacaa cggtaatttt cttacctttg atctggtcga tttctaccgg gccgttccag
   128881 ccttcgatag ttactttttc cgtttcgagg tcggcggtcg cttcgtaagt cagcaggcta
   128941 cccacttcgg aagcgagttc gcgaaagcgc ttggtgctga tatcttgctc acgcatcagt
   129001 cccagcttgt gtttgacgag tgggtgtttg acttccacga tcttcatact ctttctcctt
   129061 tgaggggcag ccacaaaaaa atcgacggat tatacctcct ttcttcaggg cggcaatatt
   129121 attttcgttg actttagtca aaatgataac agtttgagat aaagttattt tatattcaaa
   129181 tggttatgaa agaagattat tccatccgaa aactaacctt tgccctggca caagtcttct
   129241 ttcgccgcgc gcccggggaa aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg
   129301 tttgctttcc ctgttagaat tgcgccgaat tttatttttc taccgcaagt aacgcgtggg
   129361 gacccaagca gtgaccgata aaacctctct tagctacaaa gatgccggtg ttgatattga
   129421 cgcgggtaat gctctggttg gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga
   129481 agtgatgggc ggtctgggcg gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga
   129541 acccgtgctg gtttccggca ctgacggcgt aggtaccaag ctgcgtctgg caatggactt
   129601 aaaacgtcac gacaccattg gtattgatct ggtcgccatg tgcgttaatg acctggtggt
   129661 gcaaggtgcg gaaccgctgt ttttcctcga ctattacgca accggaaaac tggatgttga
   129721 taccgcttca gcggtgatca gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct
   129781 ggtgggtggc gaaacggcag aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc
   129841 gggtttctgc gtgggcgtgg tagaaaaatc agaaatcatc gacggctcta aagtcagcga
   129901 cggcgatgtg ctgattgcac tcggttccag cggtccgcac tcgaacggct attcgctggt
   129961 gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc accgaacttg atggtaagcc
   130021 attagccgat catctgctgg caccgacccg catttacgtg aagtcagtgc tggagttgat
   130081 tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc ggcggcttct gggaaaacat
   130141 tccgcgcgta ttgccagata atacccaggc agtgattgat gaatcttcct ggcagtggcc
   130201 ggaagtgttc aactggctgc aaacggcagg taacgttgag cgccatgaaa tgtatcgcac
   130261 cttcaactgc ggcgtcggga tgattatcgc cctgcctgct ccggaagtgg acaaagccct
   130321 cgccctgcta aatgccaacg gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga
   130381 ttccgaacaa cgcgtggtta tcgaataatg aatattgtgg tgcttatttc cggcaacgga
   130441 agtaatttac aggcaattat tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg
   130501 gcagttttca gcaataaggc cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt
   130561 gcaacgcata cgctcatcgc cagcgcgttt gacagtcgtg aagcctatga ccgggagttg
   130621 attcatgaaa tcgacatgta cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt
   130681 ctcagcccgg cgtttgtctc ccactatgcc gggcgtttgc tgaacattca cccttctctg
   130741 ctgccgaaat atcccggatt acacacccat cgtcaggcgc tggaaaatgg cgatgaagag
   130801 cacggtacat cggtgcattt cgtcaccgat gaactggacg gtggcccggt tattttacag
   130861 gcgaaagtcc cggtatttgc tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc
   130921 caggaacacg ctatttatcc actggtgatt agctggtttg ccgatggtcg tctgaaaatg
   130981 cacgaaaacg ccgcgtggct ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac
   131041 gagtaatgcc cccgtagtta aagctccagc tctgccgctg gcgtttttca attcacctgt
   131101 aaatcgcaag ctccagcagt ttttttcccc cttttctggc atagttggac atctgccaat
   131161 attgctcgcc ataatatcca ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg
   131221 ggtcaggaaa agctatacat cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg
   131281 cttcaggaag cggcggacaa atctaacccg ctgattgaaa ggatgcgttt cctggggatc
   131341 tattccaata accttgatga gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc
   131401 attattagcg aagaacaagg ctccaacgct cattcccgcc atttactggg caaaattcag
   131461 tcccgggtgc tgaaagccga tcaggaattc gacggcctct acaacgagct attgctggag
   131521 atggcgcgca accagatctt cctgattaat gaacgccagc tctccgtcaa tcaacaaaac
   131581 tggctgcgtc attattttaa gcagtatctg cgtcagcaca ttacgccgat tttaatcaat
   131641 cctgacactg acttagtgca gttcctgaaa gatgattaca cctatctggc ggtggaaatt
   131701 atccgtagcg atagcatccg ttacgcgctg ctggagatcc catcagataa agtgccgcgc
   131761 tttgtgaatt taccgccaga agcgccgcgt cgacgcaagc cgatgattct tctggataac
   131821 attctgcgtt actgccttga tgatattttc aaaggcttct ttgattatga cgcgctgaat
   131881 gcctattcaa tgaagatgac ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc
   131941 agcctgatgg agttgatgtc ttccagtctc aagcagcgtt taactgctga gccggtgcgt
   132001 tttgtttatc agcgcgatat gcccaatgcg ctggttgaag tgttacgcga aaaactgact
   132061 atttcccgct acgactccat cgtccccggc ggtcgttatc ataattttaa agactttatt
   132121 aatttcccca atgtcggcaa agccaatctg gtgaacaaac cactgccgcg tttacgccat
   132181 atttggtttg ataaagccca gttccgcaat ggttttgatg ccattcgcga acgcgatgtg
   132241 ttgctctatt atccttatca cacctttgag catgtgctgg aactgctgcg tcaggcttcg
   132301 ttcgacccga gcgtactggc gattaaaatt aacatttacc gcgtggcgaa agattcacgc
   132361 atcatcgact cgatgatcca cgccgcacat aacggtaaga aagtcactgt ggtggttgag
   132421 ttacaggcgc gtttcgacga agaagccaac attcactggg cgaagcgcct gaccgaagca
   132481 ggcgtgcacg ttatcttctc tgcgccgggg ctgaaaattc acgccaaact gttcctgatt
   132541 tcacgtaaag aaaacggtga agtggtccgt tacgcacaca tcgggaccgg gaactttaac
   132601 gaaaaaaccg cgcgtcttta tactgactat tcgttgctga ccgcagatgc gcgtatcacc
   132661 aacgaagtac ggcgggtatt taactttatt gaaaacccat accgcccggt gacatttgat
   132721 tatttaatgg tgtcaccgca aaactctcgc cgtctgttat atgaaatggt agaccgcgaa
   132781 atcgccaacg cgcagcaagg gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc
   132841 gataaaggcc tggttgatcg tctgtatgcg gcctccagct ccggcgtacc ggttaatctg
   132901 ctggttcgcg gaatgtgttc gctgatcccc aatctggaag gcattagcga caacattcgt
   132961 gccatcagta ttgttgaccg ttaccttgaa catgaccggg tttatatttt tgaaaatggc
   133021 ggcgataaaa aggtctacct ttcttccgcc gactggatga cgcgcaatat tgattatcgt
   133081 attgaagtgg cgacaccgct gctcgatccg cgcctgaagc agcgggtgct ggacatcatc
   133141 gacatattgt tcagcgatac ggtcaaagca cgttatatcg ataaagaact cagtaatcgc
   133201 tacgttcccc gcggcaatcg ccgcaaagta cgggcgcagt tggcgattta cgactacatc
   133261 aaatcactcg aacaacctga ataaccctat gccaatacac gataaatccc ctcgtccgca
   133321 ggagtttgct gcggtcgatc ttggttcaaa cagttttcac atggtcatag cccgtgtggt
   133381 agatggtgcc atgcagatta ttggccgcct gaaacagcgg gtgcatctgg cggacggcct
   133441 ggggccagat aatatgttga gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct
   133501 gtttgccgaa cggctacaag ggttttctcc tgccagcgtc tgtatagtcg gcacccatac
   133561 gctgcgtcag gcgctgaacg ccactgactt tctgaaacgc gcggaaaagg tcattcccta
   133621 cccgattgaa attatttccg gtaatgaaga agcccgtctg atttttatgg gcgtggaaca
   133681 tacccaaccg gaaaaaggtc gcaaactggt tattgatatt ggcggcggat ctacggaact
   133741 ggtgattggt gaaaatttcg aacctattct cgttgaaagc cgccggatgg gttgtgtcag
   133801 ctttgcccag ctttattttc ctggcggggt catcaataaa gagaattttc agcgcgctcg
   133861 catggcggca gcacaaaaac tggaaacttt aacctggcaa ttccgtattc agggctggaa
   133921 cgttgcaatg ggcgcttccg gtaccataaa agccgcccat gaagtgttaa tggaaatggg
   133981 cgagaaagac gggataatta ccccggaacg tctggaaaaa ctggtaaaag aaattttacg
   134041 tcaccgtaat ttcgcatcgc tgagtttacc gggtctttcc gaagagcgga aaacagtctt
   134101 cgttcctgga ctggcgattt tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg
   134161 cctttctgac ggggcgttac gcgaaggcgt actgtatgaa atggaaggac gtttccgtca
   134221 tcaggatgtg cgtagtcgca ccgccagcag cctcgccaac cagtatcaca tagacagcga
   134281 acaggcccga cgagtgctgg ataccactat gcaaatgtac gaacagtggc gggaacagca
   134341 accgaagctg gcgcatccgc aactggaggc gctactgcga tgggccgcca tgctgcatga
   134401 ggtcgggttg aatatcaacc acagcggttt gcatcgccac tccgcttata ttctgcaaaa
   134461 cagtgacttg ccgggtttta atcaggaaca gcagctgatg atggcgacac tggtgcgcta
   134521 tcaccgtaaa gcgattaagc tcgacgatct gccgcgcttt accttgttta agaagaaaca
   134581 gttcctgcca ctgatacagc tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc
   134641 aaccaccaca ccgccaacat tgacactgat tactgatgac agtcactgga cactgcgttt
   134701 cccgcatgac tggtttagtc agaatgcgct ggtactgctt gatctggaaa aggagcaaga
   134761 atactgggaa ggcgtggctg gctggcggtt gaaaattgaa gaagaaagta ccccagaaat
   134821 cgccgcttaa tgctttgcgg gccgacgaga aatgtcggcc cgcattattc aggcactttc
   134881 gcgaatgggt tcgatttcat tcagcgtatc aattaacggt tgcggcttac caataagata
   134941 accctgcata taatcgatcc ccaaagagag caccgcctcg cggatctctt cgttttcaac
   135001 gtactctgcc actacccgca ttttcttcat tcgcgccaga tggcaaatcg acgccacaat
   135061 ctgataatcc agactatttg acacaatatt gcggataaag ctgccgtcaa ttttaagcag
   135121 atcggcattc acatttttaa gccgtgcata gctggcgtag ccggtgccga aatcatcaat
   135181 cgcaatctgg cagcctaatt cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt
   135241 ggtcagagca ttactttcgg tgacttcaaa aataagttgc cacgcttcaa tctgatattt
   135301 agccagcaac tgactgactt caacaggaaa acgagcctga cataccgagg ttggagacag
   135361 attaatagca aaacggtgag cgggcatctt cgctctgttt tcagccataa attgcagcgt
   135421 atgctcaatg acccacatgt cgatactcga cgataaacca aattcgtgcg cgaccggcaa
   135481 gaagctaccg gggccgatca gttcatcatt ctcacctttc atgcgcagaa gaatttcatg
   135541 gtaaacatca ccacgcatac cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc
   135601 cagcgcctgc tgtagtcgat tcatcatcgc gactttatct ttcaattcgc gttgcaaata
   135661 cattgccccg caacgctgca tattttccgg ggcgttggtc acgatggaaa gttcggcgac
   135721 cgtatttagc tctcccagca gcaggtagat atgattcact ggcgagcgca catagcagta
   135781 actgacgcca atctgcggtt gcatcggcat cccatcccaa aagaaacgaa attgcttgag
   135841 atgactatcc agtgcggtaa tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag
   135901 atcgttaccc gaaagctgat aaacatcttc acccggttcc agcaagggtg acagccagtg
   135961 agaaagtttt tgcttgt
//
LOCUS       DAJTUM010000002        95425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTUM010000002 DAJTUM010000000
VERSION     DAJTUM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO471_000131"
     CDS             complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO471_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6241552.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1511..2164
                     /gene="rluE"
                     /locus_tag="OO471_000132"
     CDS             1511..2164
                     /gene="rluE"
                     /locus_tag="OO471_000132"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6241553.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2174..2635
                     /gene="nudJ"
                     /locus_tag="OO471_000133"
     CDS             2174..2635
                     /gene="nudJ"
                     /locus_tag="OO471_000133"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6241554.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2689..3795
                     /gene="mnmA"
                     /locus_tag="OO471_000134"
     CDS             2689..3795
                     /gene="mnmA"
                     /locus_tag="OO471_000134"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6241555.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3831..4472
                     /gene="hflD"
                     /locus_tag="OO471_000135"
     CDS             3831..4472
                     /gene="hflD"
                     /locus_tag="OO471_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6241556.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4476..5846
                     /gene="purB"
                     /locus_tag="OO471_000136"
     CDS             4476..5846
                     /gene="purB"
                     /locus_tag="OO471_000136"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6241557.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6015..6686
                     /gene="phoP"
                     /locus_tag="OO471_000137"
     CDS             6015..6686
                     /gene="phoP"
                     /locus_tag="OO471_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6241558.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6686..8146
                     /gene="phoQ"
                     /locus_tag="OO471_000138"
     CDS             6686..8146
                     /gene="phoQ"
                     /locus_tag="OO471_000138"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6241559.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8943..9224)
                     /locus_tag="OO471_000139"
     CDS             complement(8943..9224)
                     /locus_tag="OO471_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241560.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9238..10899)
                     /locus_tag="OO471_000140"
     CDS             complement(9238..10899)
                     /locus_tag="OO471_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6241561.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10883..11209)
                     /locus_tag="OO471_000141"
     CDS             complement(10883..11209)
                     /locus_tag="OO471_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6241562.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLEQYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11265..11447)
                     /locus_tag="OO471_000142"
     CDS             complement(11265..11447)
                     /locus_tag="OO471_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241563.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11431..11871)
                     /locus_tag="OO471_000143"
     CDS             complement(11431..11871)
                     /locus_tag="OO471_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6241564.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11871..12167)
                     /locus_tag="OO471_000144"
     CDS             complement(11871..12167)
                     /locus_tag="OO471_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6241565.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12164..12502)
                     /locus_tag="OO471_000145"
     CDS             complement(12164..12502)
                     /locus_tag="OO471_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6241566.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12499..13710)
                     /locus_tag="OO471_000146"
     CDS             complement(12499..13710)
                     /locus_tag="OO471_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6241567.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13712..14284)
                     /locus_tag="OO471_000147"
     CDS             complement(13712..14284)
                     /locus_tag="OO471_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6241568.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14324..15481)
                     /locus_tag="OO471_000148"
                     /pseudo
     CDS             complement(14324..15481)
                     /locus_tag="OO471_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15782..16006)
                     /locus_tag="OO471_000149"
     CDS             complement(15782..16006)
                     /locus_tag="OO471_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241569.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16148..16309)
                     /locus_tag="OO471_000150"
     CDS             complement(16148..16309)
                     /locus_tag="OO471_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6241570.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16319..16624)
                     /locus_tag="OO471_000151"
     CDS             complement(16319..16624)
                     /locus_tag="OO471_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241571.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16621..16899)
                     /locus_tag="OO471_000152"
     CDS             complement(16621..16899)
                     /locus_tag="OO471_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241572.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17188..18936)
                     /locus_tag="OO471_000153"
     CDS             complement(17188..18936)
                     /locus_tag="OO471_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6241573.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18933..19232)
                     /locus_tag="OO471_000154"
     CDS             complement(18933..19232)
                     /locus_tag="OO471_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6241574.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19238..19471)
                     /locus_tag="OO471_000155"
     CDS             complement(19238..19471)
                     /locus_tag="OO471_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241575.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19473..19693)
                     /locus_tag="OO471_000156"
                     /pseudo
     CDS             complement(19473..19693)
                     /locus_tag="OO471_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19686..19907)
                     /locus_tag="OO471_000157"
     CDS             complement(19686..19907)
                     /locus_tag="OO471_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241576.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19897..20097)
                     /locus_tag="OO471_000158"
     CDS             complement(19897..20097)
                     /locus_tag="OO471_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6241577.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20227..20406
                     /locus_tag="OO471_000159"
     CDS             20227..20406
                     /locus_tag="OO471_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241578.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20531..20860
                     /locus_tag="OO471_000160"
     CDS             20531..20860
                     /locus_tag="OO471_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241579.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20910..21098)
                     /locus_tag="OO471_000161"
     CDS             complement(20910..21098)
                     /locus_tag="OO471_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6241580.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21473..22702)
                     /locus_tag="OO471_000162"
     CDS             complement(21473..22702)
                     /locus_tag="OO471_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6241581.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22951..24072
                     /gene="roxA"
                     /locus_tag="OO471_000163"
                     /gene_synonym="ycfD"
     CDS             22951..24072
                     /gene="roxA"
                     /locus_tag="OO471_000163"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6241582.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24121..25347)
                     /gene="pepT"
                     /locus_tag="OO471_000164"
     CDS             complement(24121..25347)
                     /gene="pepT"
                     /locus_tag="OO471_000164"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6241583.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25597..26733
                     /gene="potA"
                     /locus_tag="OO471_000165"
     CDS             25597..26733
                     /gene="potA"
                     /locus_tag="OO471_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6241584.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26717..27574
                     /gene="potB"
                     /locus_tag="OO471_000166"
     CDS             26717..27574
                     /gene="potB"
                     /locus_tag="OO471_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6241585.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27571..28365
                     /gene="potC"
                     /locus_tag="OO471_000167"
     CDS             27571..28365
                     /gene="potC"
                     /locus_tag="OO471_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6241586.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28362..29408
                     /gene="potD"
                     /locus_tag="OO471_000168"
     CDS             28362..29408
                     /gene="potD"
                     /locus_tag="OO471_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6241587.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29564..30385)
                     /gene="cobB"
                     /locus_tag="OO471_000169"
     CDS             complement(29564..30385)
                     /gene="cobB"
                     /locus_tag="OO471_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6241588.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30401..31312)
                     /gene="nagK"
                     /locus_tag="OO471_000170"
     CDS             complement(30401..31312)
                     /gene="nagK"
                     /locus_tag="OO471_000170"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6241589.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31341..32585)
                     /gene="lolE"
                     /locus_tag="OO471_000171"
     CDS             complement(31341..32585)
                     /gene="lolE"
                     /locus_tag="OO471_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6241590.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32585..33286)
                     /gene="lolD"
                     /locus_tag="OO471_000172"
     CDS             complement(32585..33286)
                     /gene="lolD"
                     /locus_tag="OO471_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6241591.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33279..34478)
                     /gene="lolC"
                     /locus_tag="OO471_000173"
     CDS             complement(33279..34478)
                     /gene="lolC"
                     /locus_tag="OO471_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6241592.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34740..35813
                     /locus_tag="OO471_000174"
     CDS             34740..35813
                     /locus_tag="OO471_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6241593.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35941..39387
                     /gene="mfd"
                     /locus_tag="OO471_000175"
     CDS             35941..39387
                     /gene="mfd"
                     /locus_tag="OO471_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6241594.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39530..40492
                     /gene="ldtC"
                     /locus_tag="OO471_000176"
     CDS             39530..40492
                     /gene="ldtC"
                     /locus_tag="OO471_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6241595.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40575..40787)
                     /locus_tag="OO471_000177"
     CDS             complement(40575..40787)
                     /locus_tag="OO471_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6241596.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41072..41704
                     /gene="comR"
                     /locus_tag="OO471_000178"
     CDS             41072..41704
                     /gene="comR"
                     /locus_tag="OO471_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6241597.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41766..42305)
                     /locus_tag="OO471_000179"
     CDS             complement(41766..42305)
                     /locus_tag="OO471_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6241598.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42532..43836)
                     /gene="ndh"
                     /locus_tag="OO471_000180"
     CDS             complement(42532..43836)
                     /gene="ndh"
                     /locus_tag="OO471_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6241599.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44244..44786)
                     /locus_tag="OO471_000181"
     CDS             complement(44244..44786)
                     /locus_tag="OO471_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6241600.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44809..45834)
                     /gene="nagZ"
                     /locus_tag="OO471_000182"
     CDS             complement(44809..45834)
                     /gene="nagZ"
                     /locus_tag="OO471_000182"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6241601.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45845..46669)
                     /gene="thiK"
                     /locus_tag="OO471_000183"
     CDS             complement(45845..46669)
                     /gene="thiK"
                     /locus_tag="OO471_000183"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6241602.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46650..47291)
                     /gene="lpoB"
                     /locus_tag="OO471_000184"
     CDS             complement(46650..47291)
                     /gene="lpoB"
                     /locus_tag="OO471_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6241603.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47306..47683)
                     /locus_tag="OO471_000185"
     CDS             complement(47306..47683)
                     /locus_tag="OO471_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6241604.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47686..48045)
                     /gene="hinT"
                     /locus_tag="OO471_000186"
     CDS             complement(47686..48045)
                     /gene="hinT"
                     /locus_tag="OO471_000186"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6241605.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48384..50569
                     /gene="fhuE"
                     /locus_tag="OO471_000187"
                     /pseudo
     CDS             48384..50569
                     /gene="fhuE"
                     /locus_tag="OO471_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50629..52062)
                     /gene="ptsG"
                     /locus_tag="OO471_000188"
     CDS             complement(50629..52062)
                     /gene="ptsG"
                     /locus_tag="OO471_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6241606.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52357..53154)
                     /locus_tag="OO471_000189"
     CDS             complement(52357..53154)
                     /locus_tag="OO471_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6241607.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53165..54169)
                     /gene="holB"
                     /locus_tag="OO471_000190"
     CDS             complement(53165..54169)
                     /gene="holB"
                     /locus_tag="OO471_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6241608.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54166..54807)
                     /locus_tag="OO471_000191"
     CDS             complement(54166..54807)
                     /locus_tag="OO471_000191"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6241609.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54797..55819)
                     /gene="yceG"
                     /locus_tag="OO471_000192"
     CDS             complement(54797..55819)
                     /gene="yceG"
                     /locus_tag="OO471_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6241610.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55822..56631)
                     /gene="pabC"
                     /locus_tag="OO471_000193"
     CDS             complement(55822..56631)
                     /gene="pabC"
                     /locus_tag="OO471_000193"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6241611.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56751..57992)
                     /gene="fabF"
                     /locus_tag="OO471_000194"
     CDS             complement(56751..57992)
                     /gene="fabF"
                     /locus_tag="OO471_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6241612.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58079..58315)
                     /gene="acpP"
                     /locus_tag="OO471_000195"
     CDS             complement(58079..58315)
                     /gene="acpP"
                     /locus_tag="OO471_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6241613.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58526..59260)
                     /gene="fabG"
                     /locus_tag="OO471_000196"
     CDS             complement(58526..59260)
                     /gene="fabG"
                     /locus_tag="OO471_000196"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6241614.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59273..60202)
                     /gene="fabD"
                     /locus_tag="OO471_000197"
     CDS             complement(59273..60202)
                     /gene="fabD"
                     /locus_tag="OO471_000197"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6241615.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60218..61171)
                     /gene="fabH"
                     /locus_tag="OO471_000198"
     CDS             complement(60218..61171)
                     /gene="fabH"
                     /locus_tag="OO471_000198"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6241616.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61239..62309)
                     /gene="plsX"
                     /locus_tag="OO471_000199"
     CDS             complement(61239..62309)
                     /gene="plsX"
                     /locus_tag="OO471_000199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6241617.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62390..62563)
                     /gene="rpmF"
                     /locus_tag="OO471_000200"
     CDS             complement(62390..62563)
                     /gene="rpmF"
                     /locus_tag="OO471_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6241618.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62615..63136)
                     /gene="yceD"
                     /locus_tag="OO471_000201"
     CDS             complement(62615..63136)
                     /gene="yceD"
                     /locus_tag="OO471_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6241619.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63335..63919
                     /gene="yceF"
                     /locus_tag="OO471_000202"
     CDS             63335..63919
                     /gene="yceF"
                     /locus_tag="OO471_000202"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6241620.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64031..64990)
                     /gene="rluC"
                     /locus_tag="OO471_000203"
     CDS             complement(64031..64990)
                     /gene="rluC"
                     /locus_tag="OO471_000203"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6241621.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65563..68754
                     /gene="rne"
                     /locus_tag="OO471_000204"
     CDS             65563..68754
                     /gene="rne"
                     /locus_tag="OO471_000204"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6241622.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68951..69893)
                     /gene="flgL"
                     /locus_tag="OO471_000205"
                     /pseudo
     CDS             complement(68951..69893)
                     /gene="flgL"
                     /locus_tag="OO471_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69905..71547)
                     /gene="flgK"
                     /locus_tag="OO471_000206"
                     /pseudo
     CDS             complement(69905..71547)
                     /gene="flgK"
                     /locus_tag="OO471_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71613..72554)
                     /gene="flgJ"
                     /locus_tag="OO471_000207"
     CDS             complement(71613..72554)
                     /gene="flgJ"
                     /locus_tag="OO471_000207"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6241623.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72554..73651)
                     /gene="flgI"
                     /locus_tag="OO471_000208"
     CDS             complement(72554..73651)
                     /gene="flgI"
                     /locus_tag="OO471_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6241624.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73663..74361)
                     /gene="flgH"
                     /locus_tag="OO471_000209"
     CDS             complement(73663..74361)
                     /gene="flgH"
                     /locus_tag="OO471_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6241625.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74414..75196)
                     /gene="flgG"
                     /locus_tag="OO471_000210"
     CDS             complement(74414..75196)
                     /gene="flgG"
                     /locus_tag="OO471_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6241626.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75334..76085)
                     /gene="flgF"
                     /locus_tag="OO471_000211"
                     /pseudo
     CDS             complement(75334..76085)
                     /gene="flgF"
                     /locus_tag="OO471_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76105..77313)
                     /gene="flgE"
                     /locus_tag="OO471_000212"
     CDS             complement(76105..77313)
                     /gene="flgE"
                     /locus_tag="OO471_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6241627.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77338..78033)
                     /gene="flgD"
                     /locus_tag="OO471_000213"
     CDS             complement(77338..78033)
                     /gene="flgD"
                     /locus_tag="OO471_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6241628.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78045..78448)
                     /gene="flgC"
                     /locus_tag="OO471_000214"
                     /pseudo
     CDS             complement(78045..78448)
                     /gene="flgC"
                     /locus_tag="OO471_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78452..78868)
                     /gene="flgB"
                     /locus_tag="OO471_000215"
     CDS             complement(78452..78868)
                     /gene="flgB"
                     /locus_tag="OO471_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6241629.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79023..79682
                     /gene="flgA"
                     /locus_tag="OO471_000216"
     CDS             79023..79682
                     /gene="flgA"
                     /locus_tag="OO471_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6241630.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79758..80051
                     /gene="flgM"
                     /locus_tag="OO471_000217"
     CDS             79758..80051
                     /gene="flgM"
                     /locus_tag="OO471_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6241631.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80056..80472
                     /gene="flgN"
                     /locus_tag="OO471_000218"
     CDS             80056..80472
                     /gene="flgN"
                     /locus_tag="OO471_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6241632.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80512..82047)
                     /gene="murJ"
                     /locus_tag="OO471_000219"
     CDS             complement(80512..82047)
                     /gene="murJ"
                     /locus_tag="OO471_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6241633.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82247..83080)
                     /locus_tag="OO471_000220"
     CDS             complement(82247..83080)
                     /locus_tag="OO471_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6241634.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83082..83729)
                     /locus_tag="OO471_000221"
     CDS             complement(83082..83729)
                     /locus_tag="OO471_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6241635.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83740..84324)
                     /gene="rimJ"
                     /locus_tag="OO471_000222"
     CDS             complement(83740..84324)
                     /gene="rimJ"
                     /locus_tag="OO471_000222"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6241636.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84560..85768
                     /gene="mdtH"
                     /locus_tag="OO471_000223"
     CDS             84560..85768
                     /gene="mdtH"
                     /locus_tag="OO471_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6241637.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85832..86479
                     /gene="grxB"
                     /locus_tag="OO471_000224"
     CDS             85832..86479
                     /gene="grxB"
                     /locus_tag="OO471_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6241638.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86613..87173
                     /locus_tag="OO471_000225"
     CDS             86613..87173
                     /locus_tag="OO471_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6241639.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87279..88325
                     /gene="pyrC"
                     /locus_tag="OO471_000226"
     CDS             87279..88325
                     /gene="pyrC"
                     /locus_tag="OO471_000226"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6241640.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88399..88644
                     /gene="dinI"
                     /locus_tag="OO471_000227"
     CDS             88399..88644
                     /gene="dinI"
                     /locus_tag="OO471_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6241641.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88934..89188
                     /gene="bssS"
                     /locus_tag="OO471_000228"
     CDS             88934..89188
                     /gene="bssS"
                     /locus_tag="OO471_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6241642.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89303..90421
                     /gene="solA"
                     /locus_tag="OO471_000229"
     CDS             89303..90421
                     /gene="solA"
                     /locus_tag="OO471_000229"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6241643.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90469..90582
                     /locus_tag="OO471_000230"
     CDS             90469..90582
                     /locus_tag="OO471_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6241644.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90843..91409
                     /locus_tag="OO471_000231"
     CDS             90843..91409
                     /locus_tag="OO471_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6241645.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91413..91988
                     /locus_tag="OO471_000232"
     CDS             91413..91988
                     /locus_tag="OO471_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6241646.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92030..93082)
                     /locus_tag="OO471_000233"
     CDS             complement(92030..93082)
                     /locus_tag="OO471_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6241647.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93307..94227
                     /gene="lpxL"
                     /locus_tag="OO471_000234"
     CDS             93307..94227
                     /gene="lpxL"
                     /locus_tag="OO471_000234"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6241648.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94399..94596
                     /locus_tag="OO471_000235"
     CDS             94399..94596
                     /locus_tag="OO471_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6241649.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94620..>95425
                     /locus_tag="OO471_000236"
     CDS             94620..>95425
                     /locus_tag="OO471_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6241650.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAII"
BASE COUNT        22956 a        25292 c        23547 g        23630 t
ORIGIN      
        1 atatgctcga tatgctatta ataatatcca cataaataaa acaacgggcg tgttatacgc
       61 ccgtttaaat atttaacaca tgtagtgatt acatattctt gatgatcgca tcaccaaact
      121 ctgaacattt cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct
      181 tggcgttgat cgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca
      241 tatggcgtaa catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac
      301 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      361 ttgctccagg ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt
      421 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      481 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggta ttcgggtttt
      541 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaattcttca cgcgccagct
      601 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      661 gggtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt
      721 tggtgccttc ttcagaacac ggcttaatac cgataccgca atgttccggg aagcgaattt
      781 tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtcag
      841 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
      901 tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc
      961 agatgtagag atccagttcc tgacgcaggg caacgttgag agaacgaata ccgccaccaa
     1021 ccggagtggt cagcgggcct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
     1081 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
     1141 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
     1201 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1261 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1321 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1381 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1441 gatttgtaat tgacttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1501 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1561 aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac atgaaaacca
     1621 gcccacgcgt gtgatcctct tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1681 aaccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1741 ccttgaccgc gatagcgaag ggttgctggt gctgaccaat aacggcgcgt tgcaggcgcg
     1801 tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc
     1861 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     1921 gcccgcaggc gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat
     1981 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     2041 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     2101 tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga
     2161 ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     2221 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2281 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac
     2341 cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2401 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2461 tcatgacagc gatatcgact gctgccgttg ggtcagcgcc gaagaaattt tacaggcgtc
     2521 aaatcttcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg ggcaacgtta
     2581 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2641 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2701 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2761 ttgcaacaac agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac
     2821 ggtgaggaat attgcacagc agcagcggat ctggctgatg cccaggcggt ctgcgacaag
     2881 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     2941 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaag
     3001 gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     3061 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     3121 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     3181 caaagcctgt tcccggtagg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3241 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3301 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3361 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3421 ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa
     3481 gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct
     3541 gtcgggttga ttgcccagca gctgcactgg gtcgatcgcg aaccattcac cggcactatg
     3601 cgttgcacgg taaaaacccg ctatcgccag accgacattc cgtgcaccgt caaggcgctg
     3661 gacgacgatc gcattaaagt gattttcgat gagccggttg ccgccgtgac gccgggccag
     3721 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3781 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3841 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     3901 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     3961 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     4021 tcgggctgga aaccctgctc ggcgtgctca atgccagcag ttgccagggc ttaaacgccg
     4081 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     4141 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     4201 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4261 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4321 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4381 tcggcggcgg acgtctacaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4441 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4501 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4561 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4621 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4681 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4741 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4801 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4861 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     4921 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     4981 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     5041 accgtatgga gcgccagcac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     5101 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     5161 tcagcgaaga gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg
     5221 aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga
     5281 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5341 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa
     5401 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     5461 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5521 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5581 tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gcagaaccaa
     5641 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctta
     5701 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc
     5761 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta
     5821 tcacgatggt tgatgagctg aaataagcct caaatcagtg ccggatggcg atgctgtccg
     5881 gcctgcttat taagattatc cactttttct tttttcactt tacctcccct ccccgctggt
     5941 ttatttaatg tttaccccca taaacacata atcgcgttac actattttaa taattaagac
     6001 agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc
     6061 ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag
     6121 ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgcctg
     6181 acgaggacgg tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc
     6241 tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg
     6301 atgattacgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa
     6361 tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc
     6421 tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca
     6481 ccattatgga aacgttgata cgcaataatg gcaaagtggt cagcaaagat tcgttaatgc
     6541 tacaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac
     6601 gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt cattaccacc gttcgcggcc
     6661 agggctacct gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc
     6721 gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta
     6781 cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt
     6841 acgtggcgag agcaatctgt tctataccct tgcgcagtgg gaaaacaata agttgcatgt
     6901 cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa
     6961 cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaaaatga tccagcctga
     7021 ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt
     7081 gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga
     7141 cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc ggatgccaaa
     7201 attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg
     7261 gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt
     7321 cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga
     7381 agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg
     7441 aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct
     7501 caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc
     7561 tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag
     7621 ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt
     7681 gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa
     7741 caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt
     7801 tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa
     7861 atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt
     7921 ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg
     7981 tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat
     8041 caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat
     8101 ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccttttac
     8161 cactacatca agtcattgat tcgatcaaac aattttcatt gaaaaagtgc caaaaatcgc
     8221 aaattgacta caccattaac tacaccgttc ggtgcactgt atgaaacaac gtggaacaaa
     8281 tagacacaag aaatatacag gcgggtaatc tttccagggg gaagcgcacc aattcatgag
     8341 gggcgttaat gtcgatatgg ggatccccat aacggggcta ccggattttt tttccggtta
     8401 acctttaatc aggctggtgg gttttacata cccttgatca ccgtaatgat gatccagatg
     8461 tgcacctttt ccaccaatgg cggaaaagct ccaaccgtaa cgatgatcgc tatggttgcc
     8521 tcgatggtct gcgtggtcat tatgaccgtt cagccagtga ccttaatcag aatttttcgc
     8581 taaaaaatga cgttggccac gtcatccgga aagcacaaaa tcacgtcgat ttttaatgaa
     8641 cggttaaacc gtctttttgt cacggtgtgt cactggttgt cactctccgt cacgattgag
     8701 gtggtggtca ttctgcccga ccgcgcatct ttgcgctatc gggaatatca aacagttacc
     8761 tcgccgactt cctcaaattg aggtttccga aaatatcaga gcgggttagc gattaagctg
     8821 acgagtaatt aaactgcgaa aatttcgtag tttctgaata tattccggcc tgctggttaa
     8881 cagtcctcag atgtgaggga tgtagccagc gcaattttgc gctttgcttt aatctcagtg
     8941 agttaccgcc gtaaccgctc cggcttcttc cagtggtacg tgattttctc cttctcccga
     9001 tacagttcaa cgcggcgatt gtaggccagc atttccagaa cgcggatccg tatgtcgcgc
     9061 atatccgcat cattaagctg gataccatca cggcgcatca cctcagcaac aacacgcgca
     9121 taattttcgg ctgtcacgct gtccggctgc gtggtctgtt cgtcagcctg ctggctgatt
     9181 ccagagacgc ggcggattaa tcgcagtatt tcggcttctg tcatgctgct gacctcatta
     9241 caccccgcta aattcttcca gtttctggcg gtggctgtcg cttatatcaa aagcaaaatc
     9301 ctcatgctct gcctggaatg taccaaacgc catcagcgcc gctacgctcg ggtctatctt
     9361 gttcggtgat tttttcttgt tcggcttgat attggcgttc gcgtcactct gcatcacgac
     9421 gttactcatc gaccaggaca acaccggatc gccacgatgc acaatcaccc tgcggttaac
     9481 aaaaacttcg aacgatttcg ccgccggact gaatctgagg taggtttgcg ggaacggctc
     9541 cacctcaaaa ccagccccct gtaattgcgt cctgagatgc gtggcgttcc acgtatcaaa
     9601 gcccaccagc ctgatattaa atttctccgc atcctgcatg atgtcatctc tgatccggtc
     9661 gtaatcaatg cagtcacctg gcgttgtgcg tatccagccc gctttagccc actggcgata
     9721 gacagcgcgg tttttattgg cggggttctg tagctggaac tccggcagat aatgacggga
     9781 aatcagcata atggttttac cgaccggaaa ggcatagcat acgctggaaa tatcgctggt
     9841 tgatgataag tccagccccg cgtaacactc ctgaccgtgt aaatcttcct ccgtgaacgt
     9901 tccggcacac tcagcccatg caccattacc catccacggg gtagtcccct gacaccagat
     9961 attaaatcgc tttgtcatca tctccaccca ctgcgacgga ataccccgcg ctttctggat
    10021 ggttgaggcc agtttttcac gatccacgga aacatcaata ttagggttcg cctttatcca
    10081 catcgccgga tcatcaacct cgttttcgtc gtccagttcg taaatcagta caaaaattga
    10141 atcgttaacc tcttcgccgt ccaggatctg gcagcaataa tcatagtgct gtttacaggc
    10201 tgaaacgacg ttactgcccg atgtggtaat ggcaaataac agcccttcgg gacgtgcgcc
    10261 catccccagc tcaagcgcgg aataaacgcc gttatcgggg tgtaggtggt attcgtccac
    10321 gatggaaaga ctggggtttg tcccctcgat ggttgccgct tttgctgcca gcggctttaa
    10381 caggctgttg cttttcgggt gtatcacctt atgcgcctga atattcaccc gcctgcgtaa
    10441 cggtcgggat aaaaggcaca tctgacgcgc attatcaaac acgatccgcg cctggtcacg
    10501 gctcaccgct gcggtgtaga tatcctgctg accgttctcc ataatcagaa accagttagc
    10561 cagaatcgcg gcggtcgtgg atttcgcatt tttgcgcggc acttcgataa aggcgctcgt
    10621 gtatttgcgc cgtccggtgg ccttaacctt aaagccgagg atgcacgcaa aggcgaactg
    10681 ctgccacggc tccagctcaa tgggtctgcc acgcatcggt ccttttacgt gcgggcacac
    10741 cctggaaaag gcaataaacc gctccacaac ctcacgatcg aacgtgtaaa gggggctttt
    10801 aaggtccgaa aagtaccgtt taacagcctg ttttaaccgt ttacaggccg gaattttgcc
    10861 cgtttttacg tcttctgcgt acttattcca ggcggtcaag ctcgtcctct tcttctgttt
    10921 ccggtggatt tttacggcgg cttatcgggt caaaaccgag caaggaggcc atttttatca
    10981 tcactctttc agcgtcggat tttgcgctta atgcggggtt tctgctctcg ccgccctgac
    11041 tgttaacaat gctgaacccg cgcgccgcaa ggtctgcaac ggctttccgg taaatggagt
    11101 agttgacgca atactgttcc agattgctcc agtcggcggg ggtcaggtct ccccgttccg
    11161 caagctgccg cgatttttcc cgccactgct tcacggcgat atcatccagg taggcggggt
    11221 cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc ctcatcagcg attgcggaaa
    11281 cattccataa cctcgcggtc acggtcggtt aatcgcttcg ctgtgatgcg ttctgcgcgt
    11341 cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc atgcgtcacg ctgccttata
    11401 agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgtcataca tccagttatt
    11461 gcggttagca gcccgttctt cctcttcacg aaatccacct gcggcacgct tgctttttgt
    11521 tgccggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg gttgatggtt
    11581 ccattcgggc cagaagagaa catcatcacc gccattaatc gggataatgt gatccaccac
    11641 cacggcgggc gtatatatcc ccttctcaag gcatcgcacg cataacgggt ttttactcag
    11701 atacatggcg cggtatttgt cccactgtct ggaatatcca cgcgcgcggc ggtgtcctcg
    11761 tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc tcacacttgc cggatttaac
    11821 gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt tgccacggca tcagtacccc
    11881 cccacatcac ggtaagccgt ccagagtgcg cctatcgtca tgggtacgcg tgtttttgcg
    11941 ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat gcagccaatc
    12001 tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc tgcctatgtg tatctggcac
    12061 gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc gccatcgatg
    12121 cggcaatgga gtttcacttc ttccagggtg ataagttctt ctgtcatttt tccgcgccct
    12181 cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg cagcggctgc ccgatgatat
    12241 tcagtacacg ccccgccagc ggccccgtac tgacctttat ccggctggtg gcgttgatgt
    12301 ccttccggta ccgtatccag atccttacgg taccaatcgc cagctctgcg cccgatgaaa
    12361 tggcctcctt gctgctgacc atgttcacgc ttgcccagag tgtgtgaccg tcctcccacg
    12421 tttcgagtat ttcgccggtt atatccctgg tctgtttcag ggtctgaatc gttatcctgt
    12481 cacgcagtcg ccctatgttc attccggttt ttctccctcg ctgattttta cttcctgttt
    12541 ccatgcctgg ctgaactcat caccaccatc acgcggtgac agcccttcac gttcgcgggc
    12601 ttcgttcgga cacataacgt cggattttat gccgcgctca taggtggcaa agcgttcgcc
    12661 aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc tgataccgga taccaggtac
    12721 aggcgaggcc accagcaagg cgttttttat ctgctgctca aagttcgcca gccacgggcg
    12781 catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag ttgctgtagg tgctgttgct
    12841 gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg gcgatgtctt caatggtgaa
    12901 gcgacgggag gccagccatt cggcatcctg attactcatg cccagctgct gatagctcat
    12961 ccccccttca aggatgggcg tttttccggc gtttctggcc cctttgtagc gttccagtgc
    13021 ggctaatgcc tgtttgccct tcacgccgtc cagccattcg cctgacgtga taacccctgc
    13081 cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt tgttgggcca gcccaagccc
    13141 cagcgattcg cggcagatgg ttacaggtga gcgccccata aaaccgtcat ccgtggagta
    13201 acgaaggtgg agaacctccc acggtaaata gttgcggatg tttccggtgt aggcgtcagt
    13261 gatgcagtag cgccagttat gatcacctgt ctgctccacg ttcaccgact gcggcgggta
    13321 aggatgtaaa gccgccggaa aaccatcacg cccccactga ataaccgcat aagcattacc
    13381 acttaacagg cagtagcgga tcatcattcg cttaaactgg tagggtgtct gccacgcgtt
    13441 cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca agccattcgc gggcctcctt
    13501 ccccttctca ttgcgtacca ggtacaggta acacggcatg gtagccaccg cgtcagagat
    13561 gaccgtgacg gcgttcatga cggcggggag tgattccgct gtcccagatg atacgtactc
    13621 acccgccccc gtgttcgatg tgccggccag cgccataaat tcatcaagcg tcatgctgcg
    13681 ctgctctttt tttcgtctga aaggccacat atcacacccc cgctaaatcc gcccaccagc
    13741 ggcgattatc cgcacgcggc attttttcgg ggtgctgctc atacagggaa cggcgggcca
    13801 gctccacgcc ggaatcgggg taagccggta ccgatgtaac ggtaatttcg tacagttccg
    13861 ccaccagcac ggtgcgcacg catggatctg ttgtggtatc ccatacatcc ttacgggagc
    13921 gaaagccaaa gctcatcccc gagatgtccc cacgcttaac cagttcgata acgtcgcgcc
    13981 ctgtactggt atccggtggg gtcagttcaa agcgtaaccc tgtttcgtcc tcttccagtt
    14041 tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg gtcatgctca tacaggccgc
    14101 gaacgtcatt acccgccgca agccattcag taaacgcccc ccgctggaat ttttcgtaaa
    14161 actcacccca taacagttct gaaagcttat cccagcgaac aacgtagccc gtcagcgtac
    14221 cggctccgct ggtggtgatt tccgatgacc ggatttccat actcttcata attttttcac
    14281 ccataaaaca ctaaaggggc tttttagccc cttctgtatg ctgttaatcg tcgtcttccg
    14341 gcagtgccag aatcttgatc gcgttcgaat ccaccacgcc accgcctaaa tatttttgcg
    14401 tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg tctggtgcga acacctgttt
    14461 cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag ggcaataacg ttatcaggca
    14521 taaactccag atattcgacc ggaaggccca gcaacgtatc aggatcaccc gcctgtaaac
    14581 ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt ctgaagtttt gccgccgtcg
    14641 tggagttaac cactcagacc gcgtttttgc ggtatttttt acgtaatgca aatttcaggt
    14701 cgatcagcgg gtccgcagat gtccacgcca gagattcgga aggtttaatt acctgcaacg
    14761 taccaaaatc acgctctttg tcgttcttct ctgcacgggg tacggataaa aaaccttttg
    14821 cttttttgtc accgtcgccc acaaccagat cgctttcttc ggtttccgtg aaggtgtcgc
    14881 caatctcacc cgtcagccat gaaaggatgt ccacatcgga aaaatccacg atttcctgtg
    14941 tggtgcgcgg gtacgcatag accggataca gcttaatgct cacctcgtta atctgcgggg
    15001 tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt aacggtcgca ccgccagcgg
    15061 aaacaagctg cttaaactcg ttgctgctga ttttcttcac ggtacagatg cgacgcatgg
    15121 tggattcatc cgccagcatt cgcatgattt cggtgttcag ttcagggata acggtataac
    15181 caccatcagc gggaacgcct gtacttaatg cgcgggtttc accggtcaga atgtagttac
    15241 gtagttctgc ggggtcagtg gtctggctgc ttttacctgg cttgctgcgc tcttcgtctg
    15301 caatggcttc aaggcgggaa atgtctttat cgagggattc agctttagcg cgtaattcgt
    15361 caaattttgc gccctcagca tcgttaagac tgcggttttc tttttcagcg ttctccagca
    15421 tgtcgcgcat ctgattttta atggcggttt tctgctggcg taattcgatt attctctgca
    15481 taaaaaaagt cctggggtta agtaaggaac tccaggacgc ggcaaacact caaccgtttt
    15541 tcataaggaa atcagtaatc gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag
    15601 cacattaaca ggcgggaaag tggccccagc gtcctggcac cacgggcgag aataatcatg
    15661 attcagttcg ggtaaaatat gccgatccgc tcagtgaaca acgtggaaca accacgaaca
    15721 aataatttac aaaaaataac aaaaagccgg attgctccgg ctgtatgtgc tgtatgggtg
    15781 ctcagtctct gatccctttc agaaattcta tcaatgcatc tgtctgttca ggatttactg
    15841 ccagcatttc accggatagc gcgcaacgaa caaaaccatg atgatccttt tcaatcagcg
    15901 cccccgtttc cagaaatgcg cggtaatcag tgatattcat cgtctccatg ttgtcagcat
    15961 ggtatttatc acgctgttta agtatctcat caaatttcat cggcattgcg ttttcctctg
    16021 ttgtgtctgt ttgtttcaga tagtaactat gccggaccgt gacgaaaagc cagtagtgct
    16081 ccataccgtt tcaaatgacg caaaaaaagc cggattgctc cggctgcgtg gtgtggttct
    16141 gatattccta ctctgctaat ttgctcagcc ccatgtctgc gtaagtgcga tttactgcat
    16201 ttctcaggtc tgcgtagttc tccgttggcg gctccggtgt gcccttgctg ctctgtttta
    16261 ccagcgggtc gcccaccagc tgcccgtaag ctgcaatttg tgctgtcata ttgccacctc
    16321 agtgaagcga tccggcattg ttctttttca tctcttccat cattttttca gtgagcatta
    16381 cgcactgaaa ccccatgttt ttgatgtcta cgggtttcat gcctttgtca gtggatatca
    16441 caggcagaat atcctgatac acatcgcggt cagggtaact aagcacacaa agctcccatg
    16501 atgccacggg tgaggatcct tcgtctgttt caacccacca gcctggtgta gctgcgctta
    16561 tttgcagaat cgtgatccgt ccgttgtgct tgtaaaattt ttcaccaaat tttggttcgc
    16621 tcatgattcc acctctccgg ttttaacgtt gattgttgtt acctgttccg cttcggcaat
    16681 ctcccgctct gtcagcgtgg caaaatttgc cgccgtcgtg gtcatgaatg cgcttatcag
    16741 ttcgggatgt gctttcgcgt atccttcccc cgcgtggcgg tctatcgttc tgattgccac
    16801 ctttaaagcg tgctctgtca tgtctaacgc gcgatatttc ggttctgttc tgtctctgcg
    16861 ttttttgaat gatttattaa atttccctga agtgtgcata tttattttta ccccctcgtt
    16921 taaaaagttt tgagttgtgc ctccccttgt ctaccttatc taccttagtg gccctcatgc
    16981 cagtaatggc gctgctttca gcggggtaga gtgcttttat ccactatcta ccccgtgtct
    17041 acctccctgt ctgattcagg taaaatcagg tagagagggt agatagtggg tagacagtaa
    17101 aaaaaggcta tctacctaac ttaatgcact gaattaaatg tatttttctt tactcaggta
    17161 gacagggtag acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat
    17221 tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt
    17281 cgcggctggc ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc
    17341 gtacgccata aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga
    17401 atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca
    17461 aaactcaata accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg
    17521 ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat
    17581 aatgacaggg atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact
    17641 aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt
    17701 gaatatcatc gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg
    17761 cttttcgtat ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt
    17821 tccttcgcct gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg
    17881 cgcacgtcca ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc
    17941 tgccagaagg ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc
    18001 ctcaataaac atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat
    18061 acgcatcatc ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag
    18121 cgcgttatcg cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt
    18181 ggtgatccag ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc
    18241 attggcgaac ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa
    18301 cgcttccacg gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat
    18361 caccaccatt tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca
    18421 tacgtgaacg atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag
    18481 ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt
    18541 aagccctgcg gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt
    18601 ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt
    18661 ctgaccaatc tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg
    18721 tttttttcca tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag
    18781 ccacacaggc ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc
    18841 gtgcgaccag gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc
    18901 gcgtggctgt ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc
    18961 atctttttta tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg
    19021 ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca
    19081 tattcgtaac cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc
    19141 atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca
    19201 ctggcagtta agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt
    19261 tatatctgtc gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg
    19321 ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt
    19381 caagccatat gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc
    19441 ggtctattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa
    19501 ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg
    19561 atttatattc tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg
    19621 aaagcgcatc agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac
    19681 gatcatcacg cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca
    19741 ttcagtgacc gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc
    19801 tgttcttctc cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc
    19861 gtagtgagtg cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg
    19921 acgaatacgg gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg
    19981 ttccgtccaa gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg
    20041 ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac
    20101 ggctcctagt ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt
    20161 gctttttgtg ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta
    20221 attaacgtgt caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta
    20281 gatcctgact tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt
    20341 gctgcctgga taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa
    20401 ggataaaaac tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac
    20461 acaatgacca acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga
    20521 aagaaagagc atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt
    20581 aaagaatact catcaaagga gagaacttcg cagttatggc aacgcactga accagactga
    20641 ttgcattgcc caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac
    20701 acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt
    20761 tttatgcctg aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc
    20821 cgcccacgtt ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga
    20881 tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag
    20941 gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg
    21001 aattgtccgg ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg
    21061 cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta
    21121 aagagttatt tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga
    21181 gttggcaaat gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt
    21241 taaattcatg ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta
    21301 aagagtgatt cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa
    21361 tctgtacact ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg
    21421 ttgtatctgt tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc
    21481 ttctggctac tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct
    21541 ccgtccacat atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc
    21601 gctgcattcc tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta
    21661 aatcgttcag catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat
    21721 ttagcctcct gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc
    21781 ccagcaataa gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg
    21841 cttccggtat gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc
    21901 agagcatttc ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc
    21961 atccgaacac aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt
    22021 ccagtgcctg caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg
    22081 gttttttccc aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc
    22141 gcctccggca gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag
    22201 gcgttcgctt tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca
    22261 gcggcatagc accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat
    22321 aatccacgcg gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg
    22381 ttaccggctt taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct
    22441 ctgccagcca tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag
    22501 gataatttcc cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag
    22561 tgaaatacca ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat
    22621 taagaaaggc tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca
    22681 gtttgttaag tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta
    22741 actacaccga tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca
    22801 caaatctgtt tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa
    22861 acactaattg ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg
    22921 gttgcagata ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc
    22981 gattttcttg aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat
    23041 tttattgacc cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac
    23101 agtcgactgg tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc
    23161 tacgatcatc tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat
    23221 gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat
    23281 ctgatgattt ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac
    23341 gtgtttatca ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg
    23401 aaacagcact gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat
    23461 gaagagctgg agcctggtga tattctttat attccgccag gattcccgca tgaaggctac
    23521 gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta
    23581 attagcggat ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat
    23641 ccggatgttc cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg
    23701 cgtgagatga tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag
    23761 tttatatccc agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca
    23821 gatgaaatct acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc
    23881 gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt
    23941 ccggcactgg atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg
    24001 ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc
    24061 ttcgaagggt aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc
    24121 ttacttccgt tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat
    24181 accttccaga atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt
    24241 cgggcaaggt aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt
    24301 cagttccggt tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg
    24361 atgctcaacc actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta
    24421 gcaatcagga tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg
    24481 cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac
    24541 ggtgcctttc atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct
    24601 ttcatccgcc ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac
    24661 tcctttcgcc gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc
    24721 gttgaagttt tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg
    24781 ggcatcgaag gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt
    24841 aaaggcgacg cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt
    24901 catgatttct gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt
    24961 aatcaacgtc tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc
    25021 atcgccgata cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac
    25081 atttttgccg ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat
    25141 atcgccaggg acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac
    25201 attgataagc cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc
    25261 ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt
    25321 caaaaatcgc tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt
    25381 aagaagcaag aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat
    25441 ttatcagcat taccttaatg aatataaagc taagccagta attcacaaca aagattggcg
    25501 attcaagcgg tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca
    25561 caaaccccgc atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat
    25621 aaacaaccga gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat
    25681 ggtaaagagg tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg
    25741 cttggccctt ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact
    25801 gttgattccg gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac
    25861 cgctatgtga acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa
    25921 aatgtggcct ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg
    25981 atggaagccc tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc
    26041 tctggtggtc aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg
    26101 ttgttgctgg atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac
    26161 gagctgaaag cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag
    26221 gaagaagcac tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa
    26281 gacggcacgc cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt
    26341 ggcgaaatta atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc
    26401 gccaacgttg aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa
    26461 aaactgcatg ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac
    26521 cacgctgaag ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag
    26581 tcggttgttg aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac
    26641 gatcctgact ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg
    26701 gaggtcgtac tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat
    26761 tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt
    26821 tttgacccgc gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg
    26881 tctgctcgat ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc
    26941 caccctcgcc tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca
    27001 caaggtgcgt ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat
    27061 tcgtatctac gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct
    27121 ctggctgggc gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat
    27181 cggtctggtt tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa
    27241 actggataag ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt
    27301 tatccgcatc attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat
    27361 gctgccagcg atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct
    27421 gatcggtaac gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc
    27481 caccagcatt acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc
    27541 tcgtttgctg aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt
    27601 atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt
    27661 aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg
    27721 atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg
    27781 gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt
    27841 cgcggtaagc cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg
    27901 atggcaattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg
    27961 ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc
    28021 ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt
    28081 accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg
    28141 ttaagcttta ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt
    28201 tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt
    28261 aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt
    28321 attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc
    28381 acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca
    28441 acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca
    28501 ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg
    28561 ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg
    28621 tcgataaaat gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact
    28681 tcagcaatct cgatccagac atgctcaaca agccttttga cccgaataac gactactcca
    28741 ttccgtatat ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat
    28801 ctgtcaccag ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg
    28861 acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca
    28921 ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg
    28981 cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca
    29041 tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt
    29101 ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa
    29161 acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg
    29221 ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag
    29281 aagtggcaaa cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc
    29341 agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag
    29401 gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt
    29461 ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct
    29521 tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca
    29581 acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt
    29641 cattaccaac ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg
    29701 cttcgtgtac aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga
    29761 catccgccat cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc
    29821 aaacgacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg
    29881 taacgtctcc ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt
    29941 ccccatgcat atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct
    30001 gcgttaccag caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg
    30061 cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt
    30121 gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt
    30181 cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg
    30241 cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga
    30301 aaaaaatacg ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta
    30361 accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga
    30421 aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac
    30481 gagctacagg taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga
    30541 aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc
    30601 ccagagaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc
    30661 cttgatcata aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat
    30721 acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc
    30781 cgcagcggcg taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca
    30841 tatggccaaa ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca
    30901 gcccgccgcc aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt
    30961 catcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc
    31021 gatcaagacg tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag
    31081 cggcatacag cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac
    31141 ctttacagcc aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg
    31201 cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat
    31261 caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc
    31321 cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg
    31381 gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg
    31441 tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg
    31501 gagaggaact gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt
    31561 gaaacaacta cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca
    31621 taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg
    31681 atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc
    31741 acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga
    31801 tacatatagc cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca
    31861 cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct
    31921 gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa
    31981 ctgtgatcga gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt
    32041 ggctgcatca gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc
    32101 tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct
    32161 ttaaaattgc gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc
    32221 tgttgtgggt taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga
    32281 ccggtgaaat tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc
    32341 agtgcttcct gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc
    32401 accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg
    32461 atcaataccg ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc
    32521 atgccgccgc gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc
    32581 gccattactc cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca
    32641 gttggcggct catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc
    32701 cctgcaagcg attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga
    32761 ggttaccggt aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa
    32821 tcgctacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct
    32881 ccagccccac cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct
    32941 tgccaatcag cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt
    33001 gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca
    33061 gtttgctcat cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc
    33121 ctaacaggtg cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt
    33181 caccttcgcc cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct
    33241 gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca
    33301 gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc
    33361 atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg
    33421 ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg
    33481 ccaagcgccg ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag
    33541 accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc
    33601 ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc
    33661 aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct
    33721 ttacggtcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag
    33781 tcaactctca gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa
    33841 cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg
    33901 ttggcggcga aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc
    33961 gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac
    34021 tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt
    34081 ttcacattga ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc
    34141 accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg
    34201 acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag
    34261 ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg
    34321 cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg
    34381 ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg
    34441 taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag
    34501 caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg
    34561 tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc
    34621 aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac
    34681 ctcgggtcat aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga
    34741 tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga
    34801 tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg
    34861 aagtcctgag caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg
    34921 tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa
    34981 attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc
    35041 agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg
    35101 cctccaatgc cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca
    35161 gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta
    35221 gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg
    35281 ttcccacgcc gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg
    35341 gcgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc
    35401 tgctgatggc ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc
    35461 tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac
    35521 gattcggtat gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct
    35581 acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg
    35641 cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt
    35701 tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg
    35761 atctgttgtt ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca
    35821 ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg
    35881 cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt
    35941 atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc
    36001 gagttaaccg gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt
    36061 ccggtggtac tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc
    36121 cagttcaccg atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt
    36181 ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg
    36241 atgcagcgtg gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac
    36301 agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca
    36361 ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc
    36421 gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat
    36481 cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag
    36541 cgcacgctgg aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat
    36601 aaagcggcaa ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat
    36661 ccggaacata tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg
    36721 cagccattgt tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc
    36781 ttgctcgtga atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg
    36841 gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc
    36901 tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa
    36961 actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac
    37021 ctggccgttc aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact
    37081 ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa
    37141 ctgctcgcac gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac
    37201 cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat
    37261 ctggcgctga tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat
    37321 tctcgccgca ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt
    37381 cagccggtgg tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa
    37441 gctggcggca ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat
    37501 gttccggtgt cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc
    37561 ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa
    37621 gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc
    37681 ttcgcgttta aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa
    37741 accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg
    37801 gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc
    37861 gccgctttcc tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt
    37921 cttgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc
    37981 gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg
    38041 gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt
    38101 aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag
    38161 cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca
    38221 cgtacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc
    38281 gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag
    38341 gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa
    38401 aacattcaga aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc
    38461 ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa
    38521 cgtttcaacg tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc
    38581 aacaccatta tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc
    38641 ggtcgcgtcg gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa
    38701 gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg
    38761 gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc
    38821 gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa
    38881 aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag
    38941 caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg
    39001 aacacgcgtc tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa
    39061 gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg
    39121 ctggatattg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt
    39181 aatgagaaag gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg
    39241 attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa
    39301 tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt
    39361 gaactggaag agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg
    39421 caacatttac aaattctttg cactttcctg cactatccga cactgtcacc atccataatt
    39481 caggcttatc tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa
    39541 aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct
    39601 accggcaaaa gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa
    39661 caaatttcat gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa
    39721 cgtcggcttt ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc
    39781 gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat
    39841 tgtgatcaac attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac
    39901 cgtgtatcca ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac
    39961 caccgtttca gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg
    40021 ttataaagca cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat
    40081 gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa
    40141 cgccgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga
    40201 tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc
    40261 gataaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc
    40321 agagaagatt gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc
    40381 atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc
    40441 cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga
    40501 taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta
    40561 atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt
    40621 acagaagtaa tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct
    40681 tccagcgatc ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct
    40741 tctggcgttg actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc
    40801 gccgcagcga tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt
    40861 cgcaagaaat tacttaacga tcgataacta aatgatagat gtgatctgga tcacatacaa
    40921 gatatttttt ataacaatca ttaattaatt taaaaatctt taaatttcag tcaaatagaa
    40981 agcaattatt tttatgggat ctggactggt gaacggaggt gattattttt ataataacct
    41041 ttcattcaca gtcaccaggt acaacggcat catggcaact gactcaacac aatgtgtaaa
    41101 aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc gcgcttgata aggccatgaa
    41161 attgttctgg caacacggtt atgaagcgac ttctcttgcg gacctcgtcg aagcaaccgg
    41221 agccaaagcg cccacgctat acgcggaatt taccaacaaa gaggggttat ttcgtgccgt
    41281 tctcgaccgc tatatcgatc gttttgccgc taagcatgaa gcacagctgt tttgtgaaga
    41341 gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc atcgccaact gctttaccag
    41401 caaagacacc ccggcaggct gcttcatgat caacaactgc accaccctct ccccagattc
    41461 aggagatata gccaatacgt tgaaatcacg ccatgcgatg caagagcgca ctttgcagca
    41521 gtttttatgt caacgacaag cgcgcgggga aatcccgacc cactgtgacg tgacacatct
    41581 ggcagaattc cttaattgta ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc
    41641 gctggaaaaa ctgatgcaga ttgccggaac gactttgcgt ttatggcccg aactggtgaa
    41701 ataagtcgca aaaaaggccc ctcagcctga gcgaatgagg ggccaaattg cagagtacag
    41761 ccttgttata ctttgttatt caaaatcagt tgcccgttac tatctagcgg gatctgcgta
    41821 cccggatcgc ggtccatgcg gattttgccc tgctgatcgc caatcttata ggtcacatca
    41881 taaccgagca ttttttctga cttgtcatac accgttttac aacgctgttg cgtagtcgtg
    41941 taagtatcgc tttcctggag agagccctgg atctggttac cggcatatcc accacccagc
    42001 gcccccacaa cagtggcgac atctttaccg cgaccaccac caaactgatg ccctatcacg
    42061 ccaccagcaa cagcgccgag caccgacccg gtaatgcgat tttcatcctg caccggtcga
    42121 cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt
    42181 ggggttgcag aaacaacctg agcgtattgc gggccccgtt caaacacgtt cagactggcc
    42241 actgccgcta cgcccaacgc agctgcgaca ccaatcccga tacccgccaa cattgattta
    42301 ttcacgggaa cacctccttc tttgcagtta tgccaatcat gcaagcgaag gagaaaagca
    42361 gctatcagac agaagatgaa aagcgcggga atgactggaa atacttaagg attcggagga
    42421 gtctgaagga atgcctgatg cgacgcttaa cgcgtcttat caggcctacc gctcgtacca
    42481 tttgtccagg gcggataagg cgtttatacc acatccgcca gtgtacgtcg attaatgcaa
    42541 cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta atccggtttt
    42601 aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gagatatata caaagcgcgc
    42661 aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc taccaacggt
    42721 ggagaagttc gacagcgata ccagcgaacc atgatcttta tactgataat ttttcagcgg
    42781 cttaccgttc atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc
    42841 ctgagcacgc ggcggaacaa agcccccttc cggacgcggg catgacgcgc agtcgccaat
    42901 agcgtaaatg tctggatcgc gagtggtttg cagcgtcggt tccaccacca gctggttgat
    42961 acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga tcccggctgc
    43021 ccataccatc agatcagcct caatatattc gccatcttta gtgtgcaggc cgccttcatc
    43081 agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg
    43141 ggccgcagca gagatacgtg gcggtaatgc aggcaaaata cgttctcccg cttctaccag
    43201 cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca gttgcttgat
    43261 cgcgttgtgc aattcagcgg agagttctac acccgtcgcg ccgccgccga caatcgcaat
    43321 gttcactttg ccatttgcgc ccaggttggc agagtatttc aggaacagat tcagcatctc
    43381 ctggtggaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct ctttgacgcc
    43441 tggcgtattg aaatcgttag aggtgctacc cagcgccatg accagggtgt cataggcgat
    43501 tttacgttcc ggaaccaaca gttcaccttt ctcgtcgcgt agttctgcaa tagtgattgt
    43561 tttcgcttca cgatcaatat caatgacgga acccaactgg aactggaaac catgattgcg
    43621 cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag tcgccacttc
    43681 gtgcagcagt ggtttccaca ggtggctgtg gttacgatcg accagcgtaa ttttggcttt
    43741 tttcttacgt cccagcttat gccccagctg tgttgccatt tccagcccac cagcaccgcc
    43801 gccgacaatc acaatctttt tcaatggcgt agtcaacgtg ccccccttaa atttattaac
    43861 caattgttaa ttaatggtta ttaacatagc tcttaattaa caacaggtta cagcaatgaa
    43921 aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt
    43981 aatcaagttt tgttgcttaa aagataggca actttaccgg gggagatcaa aaaatagagt
    44041 ttattgttta ttatttgagc aaattcggaa gggtaatata gggtgaagcg tatatcgagg
    44101 gcgtttggtc gtcctgataa gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg
    44161 gatgcggcgt ggacgcctta tccgacttac agaattcgga gcgcatcagg taccggcaag
    44221 gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat gcgctgtaaa
    44281 tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa
    44341 tgatgcaact cttcagatgt ccgctggctg ttaagtgctt catcattacg cgacaaaata
    44401 accagacaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg
    44461 gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg
    44521 ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc
    44581 aggccaacgc cgcaaattag cggacgctcg tcaacgttca gttgcaacat tttgtccact
    44641 tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt
    44701 acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg actgttagag
    44761 tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctac
    44821 tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgatcg ctttccagcg
    44881 agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg
    44941 ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca
    45001 gaccaggatc atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc
    45061 cataatcgcg gcaccttcca tcgataaatc gtcagagaaa atcacgccgt caaaccccag
    45121 ttcctgacgc aaaacggttt tcagccagta gggagaaccg ctcgccggac gcggatcaac
    45181 atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaagga
    45241 actgaagacc gacatatctt tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt
    45301 ttctttgtgt gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt
    45361 cattccggct tcatgcatac catcaataaa ccggctggca attgccaggg ctttttgtgg
    45421 atcggcatga taagaacgct cgccaatcgc cgcgctgata tgcccgacat ccagcactgg
    45481 cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc
    45541 ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt gtgctgccgg
    45601 taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac
    45661 caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta actgggcagg
    45721 atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg ccagtatttc
    45781 acgctcttcc gcgtccagtt cgtaaccttc gacatccaac attactggac ccacactgct
    45841 ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt
    45901 gatcgccggt ttgtcgccag cggtactcaa accaccctcc tttgagcatc agcagccagg
    45961 gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat
    46021 agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca
    46081 gcgcgatatc accatctcca gcatactccc agtcgatgag ttttaaccct gatgcgctat
    46141 gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa ggtcgtggtt
    46201 cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc cgcgccggat
    46261 cgctttgctg ccagtacagt tccagtaagg gcaacagcgt tattcgccag ccaaaacgtg
    46321 gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt
    46381 ttaccgcacc gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg
    46441 gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg
    46501 gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa
    46561 aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc
    46621 gcgacagcaa ttcgtcgcgc gtgatgggat tattgctgcg aaacggcacc tttacctgac
    46681 cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg
    46741 ttgccagagg cgcaggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg
    46801 gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc
    46861 gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc
    46921 gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc
    46981 agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac catcggctgc
    47041 attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt tcgatcttcg
    47101 tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc
    47161 tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca
    47221 cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc
    47281 atttttgtca tcaagattca cccctttaca aatatagata aagtctgact ttgtgcgccc
    47341 ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc
    47401 gtggcctttc cagaggatgc atctccagcc ctctggcgtc ataccagtaa aaacgataat
    47461 gaacggtaac gggttcttgc ctttcgttat aaatcgttga ggatgctgaa ggttgaatat
    47521 cagacgtcga aaggacgggc ttttctgcac tgatgcccgc agccaataaa gatgactcca
    47581 tcaccagcga ttgctcatca ttcaccggaa tttccggatg tgaacgacag cccaccagca
    47641 gtaacaacac cagagacacc agcccaaagc atccttttct catcgttaca gacctttatg
    47701 cgccagcatt ggtcccagcg gacggccacc caacaagtgc atatggatgt ggtaaacctc
    47761 ttgtccgcca tggcggttgg tattcatgat cagacgatag ccatcttcgg caataccttc
    47821 ttgctcagca atttttgccg ctacggtgat catgcgtccc agcgcctgct catgctcagc
    47881 tgacacgtcg ttcacagtcg gaatgagaat attcggaatg atcagaatat gcgttggcgc
    47941 ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc tggtagacga tatcggaggg
    48001 gatctcacga cgaataattt tgctgaatat agtttcttct gccacgacat tttccttttt
    48061 caaatagcca tgcgttgcgc catgctacgc cgcgccacac tgcgagtata gagtatggtc
    48121 gagttaaacc tgctctttca atcttaactc acgatttctt aagcaaaaaa tcgttttgct
    48181 gccgtatata tcgccattat tcccatttct gcctgcgaat gcgctgaaaa gacattctct
    48241 gtttggttta cacgacaaat atgaatgcgt atatttctca tttgcatttg catttgcagc
    48301 aaaattattc gcacactaaa tataaccgct catcaccctc ctccggcatg agcctgacga
    48361 caacataaac caagagattt caaatgcttt caacacaatt taacagggat aatcaatatc
    48421 aagtcatcac caaaccgtca ctacttgccg gttgcatagc actggcacta ttaccttctg
    48481 ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt tgagggttca gccacagctc
    48541 cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc aaaatgcaga
    48601 tgactcaaca tgatattcct cagtcggtca ctattgttag ccagcagcgg atggaagatc
    48661 agcagttaca aacgctgggc gaagtgatgg aaaacacgct ggggatcagc aaaagtcagg
    48721 cggattccga tcgtgctctt tattattccc gcggattcca gatcgataac tatatggttg
    48781 atggtatccc cacctatttt gaatcgcgct ggaatctggg cgacgcactt tctgatatgg
    48841 cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc gggacgggta
    48901 atccatctgc ggcaattaat atggttcgaa aacacgcgac ctgtcgtgaa tttaaaggcg
    48961 atgtctcggc ggaatacggt agctggaaca aagaacggta tgtggcggat ttacaaagcc
    49021 cactcaccga agacggtaaa atccgcgcgc gaattgtcgg cggctaccag aataacgact
    49081 catggctgga ccgctacaat agtgaaaaga ccttcttctc gggcattgtc gatgctgatt
    49141 taggcgatct tacgacgctt tcagccggtt acgaatatca gcgcattgat gttaatagcc
    49201 caacctgggg cggtttaccg cgctggaata ctgatggcag cagcaacagt tacgatcgcg
    49261 cacgcagtac cgcacctgac tgggcgtaca acgataaaga gatcaacaag gtctttatga
    49321 ccctgaagca gcggtttgct gatacctggc aagcgacact gaatgccacc cactctgaag
    49381 tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc
    49441 tggttgggcc atacagtaat tatggacctg gctttgatca tgtcggcggc accggttgga
    49501 acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt tatgaattgt
    49561 ttggtcgtca gcacaatcta atgtttggtg gcagttacag caaacaaaac aatcgttcag
    49621 ttcatgggcc aacatcttcc cggatgaaat tggcagtttc tacaacttta atggcaattt
    49681 cccacaagcc gactggtcat cacagagcct ggcgcaggac gataccacac atatgaaatc
    49741 gttatatgct gccactcgtg tcacccttgc cgatccgctg catctgatcc tcggcgcacg
    49801 ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc acaccacgcc
    49861 ttacgctggt ctggtgtttg acatcaatga caactggtcg acctacgcca gctatacctc
    49921 tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc caatcaccgg
    49981 taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga ccaccacgtt
    50041 agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac ctatccccgg
    50101 cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag gggtggaatt
    50161 tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa cgcgctatct
    50221 tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca cggttaaaat
    50281 gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg
    50341 gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg ccgagcaagg
    50401 tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact tctcgttaca
    50461 ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacatggaag gttctatcgt
    50521 ctacggcgca ccgcgtaatt tcagcattac cggcacgtat caattctgaa taacacctgt
    50581 aaaaaaggca gccatctggc tgccttagtc tccccaacgt cttacggatt agtggttacg
    50641 gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa tagcctgaac
    50701 accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccag cctgatccac
    50761 tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga ggttagtaat
    50821 gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg tacctgttgc
    50881 ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg ctttaatcag
    50941 cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg gtaacagcca
    51001 cagtttgctg ctgttaccag acagaacgat gaagtcgatc agaccgtgcg agaacgacgt
    51061 accgtcacgc atccccagaa gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg
    51121 gatgatgtac aggatcggcg caacgaacat gaagaagaac tcgatcggct cggtgatacc
    51181 ggtcaggaac gaggtcagcg ccgcggagat cataataccg cccactttcg cgcggttttc
    51241 tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca ttttgaacag
    51301 gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc
    51361 gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt
    51421 ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt aaatgccaaa
    51481 cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg cagaaccaat
    51541 cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc cagaaatgat
    51601 cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa tacggtagaa
    51661 acggttaaac atgtacgctg caatcgcacc ggagataatc cctccgagta cgccagtatc
    51721 cgccaagtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac
    51781 ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg atacgccatc
    51841 gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac
    51901 ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc tgaaattcgc
    51961 ggaaccgacg cccagcagaa tacctgcgat aggtagtacg gataccggca gcatcagcga
    52021 tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga gtgctcctga
    52081 gtatgggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca
    52141 gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc caatttgttt
    52201 gacggctatc acgtttcaac ttcagatcat tggagaagtt gcacattttg acaaaacata
    52261 tgtcacaata tttcaaacgc ggttattcac cgcccaattt tcggcggtga actttactcg
    52321 ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt gaaggcggga
    52381 agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca gttcttcaac
    52441 ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg caggttgatt
    52501 ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg
    52561 atccaggggg acataacgcg cagcatcgcg cagttgctcc gcattacgga aggtcacaat
    52621 gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt ctctgtcctc
    52681 tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca gaattgccag
    52741 cgtatcggca cgggcgtcac gggtatggac gataaccggc ttgttcagtt cacgaccaat
    52801 ctggatatga tggatgaagg actcttgctg acgtacttta gtttccggtg tgtaataata
    52861 atccagcccg gtttcaccca gcgctacaac accctcttct gccgccagac ggcgtaaatc
    52921 ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa atacgacgtt
    52981 gtcacgttcg cctaccagat cccgcatatg taaataaccc ggtaatgttg tggcgactgc
    53041 cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca cgtccttatg
    53101 caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa acatgatgtc
    53161 tctcttaaag atgaggaacc ggtaacacaa cgcccggttg caggtaatgc tcaatacgca
    53221 gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt gacagacatt aactgttcac
    53281 gaatgtggca aacatccccc agtatagcct gcaggcacga gggagaaaga tggtttgcca
    53341 gttcggcgac caggcccggc acatcaacat tggtcacctg cgcagcgcca tgatggcgtt
    53401 ttagcgcatc catcagcaac gttgccagcc agtgtaaacg cgccggagct tgttcatgat
    53461 taagggccgc cagcagcgaa taccagtcgc ccgatggcac gctatatgcc aacgcctgac
    53521 acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg
    53581 aaccggcgct taagcgcaat gcggcaagta atgcatcctg tgacattgtc acttcgcgtg
    53641 aaagccaagt cacggcgtac tgttctggag gcggcgcaag gtaatgtaac cgacaacgac
    53701 tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac caggtttctg
    53761 ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag
    53821 catcggttac ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg
    53881 tgacctcacg taccgcatca atgcccagcg tatttttccc tttttcggga gccagggtgt
    53941 agtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga ccgcaacttt
    54001 tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt aaagcatcat
    54061 cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg gcctgatagc
    54121 tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg cgtccaactc
    54181 cttcacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca gcggctgggt
    54241 ggcatcaatg gtatgaatac ttttatcttg tgcagccagt tccagatagc gggcgcgggt
    54301 gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc gcgcacgcgc
    54361 gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtctg ggcgaaaatc
    54421 cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac gtcctccgcc
    54481 ctgatacgcc tgagtggaaa gatcgtggcg atcgccaatc acccaggtgc cgttagccag
    54541 cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc
    54601 ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca ggcttcttaa
    54661 cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc ggatacccag
    54721 ttgctcgagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc cttccagccc
    54781 ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga taatcctgca
    54841 cagacttgtt atgactggca agattggtat taaacgtgtg accaccttta ccatcggcca
    54901 caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa tccgcccccg
    54961 gcgtagctat cgcacccggc ggcaaaccgg taatggtata ggtgttatac gctgtcggcg
    55021 tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg taaatcacgg
    55081 ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc gaggcaacct
    55141 gatcgcgttc actggcaacg gcggtttctt tttcgataat tgatgccatc gtcaccagct
    55201 gatttttatc tttataaggc agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt
    55261 tcaccatttt cttgtgaact cgcttgagta acgcgacatc ggtggtattg gcggtataca
    55321 tcaaggtgtc tggccagaac caaccttcaa tccactccgg gttttccagt tcaagtgcct
    55381 gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt gatatacgga gcctcacgca
    55441 attgcttgag gtagtcgctc agacgcatcc cttctaccag tcgcagcggg aactgtgctt
    55501 ctttaccgct ttccaacaat ttcagcatct cgcgcacggt catctgcggt gtaaagcggt
    55561 aagtcccggc tttaaagtga gaaagatccg gttcgatacg cagcagccat tgaaaaaccc
    55621 gtgggcgatt gatgatctta tcagcataaa gctgttcacc gagcgccaga cgtccggtcc
    55681 ctggcttcag ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg gcaagatggc
    55741 gaaccttcca gacgcccaca ccagcggcga tacccagtac caccagcaat aacaagataa
    55801 tcaataacac ttttttcatg actaattcgg gcgctcacaa agtggggcta aatattcata
    55861 taacgttgct gacgaaaagg tgacatcgcc acaggcacgt acgggcatca ctggcattaa
    55921 cgcattacaa ataaccatct catctgcctg caacgcctcc tccagcgagg cttgcacttc
    55981 gacaagctga taagaggatt gtgccagtaa acggatacag aattgtcgca taatgccgtt
    56041 aacacctgcc tgatccagtc gcggcgtata aactacattg cccttccgcc agaacaaatt
    56101 agccgcacag cattccgtaa cccacccttc gctgtcaagg accagcgcct catcagcgtt
    56161 tgtctgctca agatgagagc gaatcaatac ttgctcaagc cgattaagat gtttaatacc
    56221 tgcaagatga ggattgcgcc ccagccgcac cgggcttagc gccaacgtca tcccttcgtt
    56281 acgcaaacgg tcgtaatgtg caggataagc cgtaacggag agaatccgcg ttgctggtcc
    56341 gctgttcaat gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga ctttcagcac
    56401 accattttgc tgttctgctg ccagcgtttt catctcttgt tcaagctgag gccagaagtc
    56461 acaggaaatc atcaaccgct gacaagcatc ctgtagtcgc aggatatgcg ccgataacaa
    56521 actgacttta ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc
    56581 ccgatcgctt actgccagcg attcctgctt ataaccgtta attaagaaca taccggctcc
    56641 ttatcagaga attgttagtg tggcagcatg ttcactacgg aacaagtcgg aataaaagct
    56701 aagaaaaaag gcccgcaagc ggacctttta tatgggtgga aaatgacaac ttagatcttt
    56761 ttaaagatca aagaaccatt agtgccaccg aagccgaagg agttacacag cgtgtattcc
    56821 attccgctga cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg
    56881 ttatccaggt tgatggttgg cggaacagcc tgatcgcgca gcgccaggat ggagtagata
    56941 gattctactg cacccgccgc acctaacagg tgaccggtca tagatttcgt ggagcttacc
    57001 aacacacggc ttgcagcttc accgaagatg gttttcaccg cctgcgcttc agctttatcg
    57061 ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct
    57121 gcatcacgca gagcatttgc cattgccaga gctgcgcctg cgccattttc tggcggtgac
    57181 gtcatatgat aagcatcgct gctcatacca aagccgacga gttcagcgta aattttcgca
    57241 ccgcgttttt tcgcgtgttc gtactcttca agtaccagca taccggcacc atcgcccagt
    57301 acgaagccat cacgctcttt atcccacggg cggctcgccg cttgcgggtt atcattgcgg
    57361 gtagataatg cacgtgccgc gccaaaacca ccaacgccca gcggcgtact ggctttctct
    57421 gcgccacctg caaccatcac gtcagcatcg ccatacgcga taatacgcgc agcatggcca
    57481 atgttgtgca cgccggaagt acaggcggtc gcgatagaga tgcttgggcc acgcaggcca
    57541 tacatgatag tcagatgacc tgccaccatg ttcacaatcg ttgacggaac gaagaatggg
    57601 ctgatcttac gtggaccacc gttcatcaga gatgtgtggt tttcttcgat cagtccgagg
    57661 ccgccaatcc cggagccaat tgcggcacca atgcgggttg cgttctcttc cgttatttca
    57721 aggccagaat cctgcatggc ctgaacgcca gcgacaattc catattgaat gaaggcatcc
    57781 atcttgcgct gttctttgcg cgagataatg tcctcacagt taaaatcctt tactaagcca
    57841 gcaaatttcg ttgcataggc gctagtatcg aaatggtcga ttaggctgat gccactctga
    57901 ccggcaagca gagctttcca ggtagactct acggtattgc cgacaggaga caacatgccc
    57961 agtccggtca caactacacg acgcttagac acgtttgtcc tccaaggagg gaaaaatgat
    58021 tctagtggga caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt
    58081 acgcctggtg gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt
    58141 cttcgtccgg aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt
    58201 caagagaatc cgcgcccagg tcttcaacga aagaagcatt gttggtaact tcttcctgct
    58261 taacgcccag ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct
    58321 taaatttcct atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa
    58381 atttgcaact aaatcccggc aggtcttacc acgattttac gttattttga ggccttaccc
    58441 cccaataacg caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa
    58501 catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag
    58561 tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt
    58621 cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat
    58681 cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa
    58741 taccgcgtga cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg
    58801 ccgcagcgta gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga
    58861 taatacgacc atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa
    58921 cagatgaaag gttagtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca
    58981 ggttatcacg agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc
    59041 gaattttttc cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt
    59101 tgccgttggc acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc
    59161 caataacttt cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc
    59221 ttgcaccggt taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct
    59281 cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa
    59341 tgcgtttcgt caggccagta agcaccttgc ccgggccgac ttcatagaga tgttctacgc
    59401 cttgcgctgc catgtactca acagacttcg tccactgaac cgggttatac aactgacgta
    59461 ccagtgcgtc acggatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa
    59521 cagaaactgt tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg
    59581 ctggtttcat cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg
    59641 cgccagcagc tttacaggca gcgccagcac gctcaaccgc ttctttatga ccggcaataa
    59701 ccacctgtcc cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt
    59761 cacacgcttt cgcaataggc gcatcatcca gaccgatgat tgccgccata gcgcccgtgc
    59821 cttccggtac ggcttcttgc atgaacttac cgcgcatctc aaccagacgc accgcatcag
    59881 cgaaatcaat cacgccagcg caaaccagcg cggagtattc ccccaggctg tgaccggcca
    59941 tcattgccgg tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag
    60001 tcaacagcgc tggctgggtt tgccaggttt tattcagttc ttcagctgac ccctgctggg
    60061 tcagcgccca caggtcgtag cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa
    60121 ttggatagct cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga
    60181 acacaaatgc aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcagagc
    60241 cccaagtgaa tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc
    60301 cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc
    60361 gatccagcgt caccacgaca ttgtccatcg acataccgag ttttttcgcc gttgcactga
    60421 taatacgcag gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat
    60481 tcgccgccag cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt
    60541 cgttgcccgc catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca
    60601 gcgtcagcaa ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg attcccggct
    60661 cttcagaggc agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac
    60721 gatctgttgg atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca
    60781 ccgccccaga tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa cctgcacagg
    60841 ctgctgcaac gtcaaatgcc gggcaacctt taatgtccaa catgctttga atctgacaag
    60901 ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt
    60961 ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa
    61021 cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact
    61081 cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat
    61141 agctgccagt accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac
    61201 cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg
    61261 ccaccgtcca gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc
    61321 tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc
    61381 tgattggctg caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg
    61441 ttatactggt cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc
    61501 tttaataaca gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa
    61561 aggaacatcc tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca
    61621 caaaccagca catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa
    61681 ttgatagaag ggattgtttt aagcaccgct gaggcatccc gaatactgtc aagacccttt
    61741 acttcttctt caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct
    61801 tcagccagaa ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg
    61861 ttggccccta aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc
    61921 agcgccggac gctcaatccc ctccaggggc ttgagtaata attttgccag ccccatcagt
    61981 gccccggtat taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg
    62041 gccatacgca ttgaactccc acggctggcg cggatagctt gcgaaggccg ggcatcactg
    62101 gcgataactg actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta
    62161 gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta
    62221 gaattcagtg cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg
    62281 acatctaacg ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc
    62341 cagttgcctg gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga
    62401 tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac
    62461 cagaagtttt gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca
    62521 tgccacgttt ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat
    62581 tacggtttat ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata
    62641 cggcaaatgg gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt
    62701 cggacacttc acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt
    62761 catcttcaac cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat
    62821 acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg
    62881 tgtagacctg atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct
    62941 tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt
    63001 ccacatcact gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat
    63061 agataccctg gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta
    63121 attttacctt ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa
    63181 aggcagcctg tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt
    63241 ttcgttcagt gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc
    63301 tatcatcaat acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac
    63361 ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc
    63421 tgaggtcgac gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc
    63481 acaagaaaaa tcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga
    63541 ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg
    63601 tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt
    63661 taattcggcg aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca
    63721 cctaagcgag gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg
    63781 tagctttaag agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgaccc
    63841 taacacgctg gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa
    63901 aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg
    63961 tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta
    64021 tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca
    64081 tcggcgcttc gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg
    64141 cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac
    64201 ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga
    64261 tctgatgagt acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt
    64321 cttccacttt aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt
    64381 cgccgctttg cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc
    64441 actgaccgcg caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat
    64501 gcagagaacg caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt
    64561 cgagacgatg aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga
    64621 taacgccgaa gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca
    64681 ccaggatatg atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat
    64741 gtggcgaaac cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat
    64801 cacccgcttc gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcacctt
    64861 tacgcaaaat acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt
    64921 tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct
    64981 ctgttttcat ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat
    65041 gcttatattt agttttgcac cttattactt cactacgtga tcactttatt gatggttatt
    65101 aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa atggtaaaag
    65161 tcatcttgct ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg tccatccttg
    65221 ttaaaacaag aaattttacg gaataaccca ttttgcccga ccgatcatcc acgcagcaat
    65281 ggcgtaagac gtattgatct ttcaggcagt tagcgggctg cgggttgcag tccttaccgg
    65341 tagatggaaa tatttctgga gagtaatacc cagtctgttt ctttgataat tgcgctgttt
    65401 ttccgcatga aaaacgggca accgacactc tgcgcctctt tgagctgacg ataaccgtga
    65461 ggttggcgac gcgactagac acgaggccat cggttcacac ccggaaaggc gttactttgc
    65521 ccgcagctta gtcgtcaatg taagaataat gagtaagtta cgatgaaaag aatgttaatc
    65581 aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac
    65641 ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc
    65701 acccgcattg aaccgagtct ggaagctgct tttgttgatt acggcgctga acgtcacggt
    65761 ttcctcccac taaaagaaat tgcccgcgaa tatttccctg ctaactacag tgctcatggt
    65821 cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa
    65881 gaagagcgcg gcaacaaagg cgcggcatta accaccttta tcagtctggc gggtagctat
    65941 ctggttctga tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat cgaaggcgat
    66001 gaccgtaccg aattaaaaga agcactggca agccttgaac tgccggaagg catggggctt
    66061 atcgtgcgca ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga tttaagcttc
    66121 cgcctgaaac actgggaagc catcaaaaaa gccgctgaaa gccgcccggc tccgttcctg
    66181 attcatcagg agagcaacgt aatcgttcgc gccttccgcg attatttacg tcaggatatc
    66241 ggcgaaatcc ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca tatcgctgca
    66301 ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca ccggcgagat cccgctgttc
    66361 agccactacc agatcgagtc gcagatcgag tccgccttcc agcgtgaagt ccgtctgcca
    66421 tccggtggtt ccattgttat cgacagcacc gaagcgttaa cagccatcga catcaactcc
    66481 gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa cctcgaagcc
    66541 gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac
    66601 ttcatcgaca tgacgccagt acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg
    66661 gtgcgtcagg accgtgcgcg tattcaaatc agccatattt ctcgctttgg cctgctggaa
    66721 atgtcccgcc agcgcctgag cccatcactg ggtgaatcca gccatcacgt ctgcccgcgc
    66781 tgctccggta ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat tctgcgtttg
    66841 atcgaagaag aagcgctgaa agagaacacc caggaagttc acgccattgt tcctgtgcca
    66901 atcgcttctt atctgctgaa tgaaaaacgt tctgcggtga atgcgattga aacgcgtcag
    66961 gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg
    67021 ttgcgcgtgc gcaaagggga agaaacgccg accttaagct acatgctgcc gaagctgcat
    67081 gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg tcctgaacaa
    67141 cctgcactgg caacctttgc catgccggat gtaccgccag cgccaacccc agctgaacct
    67201 gccgcgactg tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc agctcctgca
    67261 caacctgggc tgttaagccg cttcttcggc gcactgaaag cgctgttcag cggtggtgaa
    67321 gaagccaaac cgaccgagca accaacaccg aaagcagaag cgaaaccgga acgtcaacag
    67381 gatcgtcgca aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg cgacacccgt
    67441 agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca
    67501 cagcagcaaa ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac ggaaaaagca
    67561 cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta gccgccgccg taacgatgat
    67621 aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa
    67681 accgaacagg aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat
    67741 cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa
    67801 gaaactgccg ctgccgaacc aattgttcag gaagctccag ctccacgcac agaactggtg
    67861 aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac agcaagaaga gaacaatgct
    67921 gataaccgtg acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc
    67981 gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg
    68041 ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg gatccgctat
    68101 ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga agaagtacaa
    68161 gtgcagccga tggtgactga ggtccctgtc gccgccgctg tcgaacctgt tgttagcgcg
    68221 ccagttgttg aagaaatggc tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa
    68281 ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg
    68341 caggtgatta ccgagtctga tgttgccgta gcccaggaag tcgcagaaca cgcagaaccg
    68401 atggttgaac cgcaggaaga gacggcagat gcagatattg aagaagttgc cgaaactgct
    68461 gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa
    68521 gtcgcagcag aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac
    68581 cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt
    68641 cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat
    68701 acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta
    68761 gctcaaagta atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac
    68821 atattaaaat tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa
    68881 tgcccgcccc ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt
    68941 taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt
    69001 tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt
    69061 ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg
    69121 aatccagcga ctccagtttg ttcagctgcg tgcctaattc cgcgcgaacg gtaaggacat
    69181 tattcagcga gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt
    69241 tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg
    69301 caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac
    69361 tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt
    69421 gcttaatact ttctgttccg ccaacataat caccgttagc ctcactaaac ggcgcagtct
    69481 ctgttttata tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca
    69541 cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg
    69601 ctggcgtaga caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca
    69661 ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt
    69721 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg
    69781 ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa
    69841 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    69901 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    69961 cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    70021 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc
    70081 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    70141 cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct
    70201 gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt
    70261 cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc
    70321 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    70381 cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    70441 gattattatc gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg
    70501 tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga
    70561 tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt
    70621 tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat
    70681 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    70741 tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc
    70801 tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg
    70861 catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt
    70921 taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc
    70981 cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt
    71041 gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata
    71101 ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg
    71161 cgccgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat
    71221 ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc
    71281 atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa
    71341 cgtatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc
    71401 gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt
    71461 atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct
    71521 catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta
    71581 ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg
    71641 taggttttgc tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta
    71701 cgggcatagt gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc
    71761 gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa
    71821 tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct
    71881 tcgccgtttt catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag
    71941 gctttgacac caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc
    72001 ccccaaccag attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc
    72061 tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc
    72121 agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt
    72181 tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct
    72241 ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg
    72301 cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc
    72361 tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc
    72421 acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc
    72481 gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg
    72541 ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg
    72601 attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca
    72661 cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat
    72721 cgatctgcgt ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc
    72781 tgacattggc ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt
    72841 cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag
    72901 cgtcctgcgg ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg
    72961 aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc
    73021 catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt
    73081 cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa
    73141 taaccgcacc attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc
    73201 ctgcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac
    73261 tgtcaacgcc cttaagcgga gtcatcaaca acgtacctcc acgcaagctt ttggcattac
    73321 ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgaca
    73381 ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa
    73441 ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct
    73501 ggtcaccagt gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta
    73561 ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta
    73621 gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt
    73681 aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac
    73741 gtattcaata cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt
    73801 gcgtggatta accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg
    73861 tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa
    73921 cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc
    73981 ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt
    74041 atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc
    74101 gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc
    74161 ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt
    74221 ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt
    74281 ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt
    74341 atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc
    74401 caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg
    74461 ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca
    74521 cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg
    74581 gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc
    74641 tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag
    74701 ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg
    74761 cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc
    74821 cgttctgatc cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca
    74881 ttacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca
    74941 gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta
    75001 atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat
    75061 aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca
    75121 ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc
    75181 ataatgaact gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg
    75241 cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg
    75301 cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc
    75361 acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc
    75421 gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc
    75481 cgacatcaca cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc
    75541 tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac
    75601 cagtttcaga cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga
    75661 gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc
    75721 ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct
    75781 gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc
    75841 aacgcaacgt ccagcgggcg agaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc
    75901 ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg
    75961 cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg
    76021 gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga
    76081 tccattgagc tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc
    76141 ctgggttttg atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac
    76201 cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca gagtgccaaa
    76261 gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac
    76321 gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc
    76381 cagcagttgg gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg
    76441 ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc
    76501 gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg gttctgcacc
    76561 gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg tgccattagc
    76621 attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc tatcctgggt
    76681 gtagacctgc cagttattat ccccggtctt cacaaaatag acgctcatgt catgagcatt
    76741 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    76801 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    76861 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc
    76921 cccttgctga atagtcggcg gcgtaccggt taccgggtaa cccgtcagct gtaaaccttg
    76981 cgtattcaac aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    77041 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    77101 tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac
    77161 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    77221 gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc
    77281 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    77341 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    77401 accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    77461 ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct
    77521 gccatcagtc aacgaaccgt cccaggtgaa actgtgaact ccggcggtca gttcaccaat
    77581 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    77641 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    77701 ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca ggttactggc
    77761 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt
    77821 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    77881 tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa aactgctttg
    77941 taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    78001 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    78061 agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg
    78121 cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttatg
    78181 tagccctttg catctgccag cggattacct ggttcataaa ccagtttgtc cggggcctga
    78241 ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    78301 ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg ctatcagcat
    78361 ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac gccgacccgg
    78421 cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    78481 tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg
    78541 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    78601 gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg atgtgctgcg
    78661 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    78721 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    78781 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    78841 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    78901 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    78961 atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    79021 aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagta
    79081 cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc gcgggggtaa
    79141 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca tgcgagcagc
    79201 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    79261 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    79321 ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgtg
    79381 gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    79441 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    79501 ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca
    79561 acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg
    79621 tatcggaaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    79681 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    79741 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct gtaagcaccg
    79801 ttcaaccgcg cgaaaccacc gacgcgccgg taacgaacag ccgggcggca aaaacaaccg
    79861 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    79921 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    79981 taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc
    80041 agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt
    80101 aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg gcagatcaac
    80161 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac
    80221 ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    80281 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    80341 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    80401 gaaccgacgc tatatggggc gaacggtcag acctcaacac cccatcgcgg cagtaaaaag
    80461 atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    80521 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    80581 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    80641 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    80701 caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg
    80761 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    80821 cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt
    80881 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    80941 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    81001 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    81061 tgaaagaatg cccaacgcaa ccgcactcgg cagtgccaac aggaaacaaa gacgcaaccc
    81121 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    81181 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg
    81241 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    81301 gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac
    81361 cacgcgcatt gctccagcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    81421 cttcaggtgc ggtagctgat acaccagctg cagaatgccg ccgaccgtaa cagcccacgc
    81481 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    81541 gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat
    81601 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    81661 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    81721 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag
    81781 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    81841 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    81901 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    81961 ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    82021 ggcggccagc gattttaata aattcatcgg tgttctaatc cggacccacg ggcaaaacgc
    82081 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctag attatggtga attgctaccg
    82141 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    82201 gcgccagcac tgcctattcg ccggcggttt gcggaactgt ctgattttac acgcattcaa
    82261 tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa
    82321 tcggtttatt cactacaccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt
    82381 cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    82441 gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt
    82501 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    82561 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    82621 gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg
    82681 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    82741 ttttccgcgc cgccagttcc accagccttt cagcatcgcg cagattttct gccagcggtt
    82801 tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtggctgg
    82861 cggtgctgga gtgcacaaaa accgcatcac aactggcggc aaggctggat aacgaatcgg
    82921 cataaggaat gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta ggcgaccagg
    82981 ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcacaa
    83041 tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    83101 agcaacgaat caagacgttg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    83161 gtctgtaaat caccgtcaac catgttcgac atagccgtta ccgccggctg atcttcaacc
    83221 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    83281 accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc cacttccgcc
    83341 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    83401 ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt cagatcgcca
    83461 aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa accgctcact
    83521 gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc ggattcactc
    83581 agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact
    83641 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aattacgcga
    83701 gcttccaggg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    83761 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    83821 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    83881 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    83941 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    84001 accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg caacgccaat
    84061 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    84121 ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    84181 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg ccagacgcca
    84241 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    84301 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    84361 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc
    84421 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    84481 ctgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    84541 ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt
    84601 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    84661 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    84721 acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    84781 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    84841 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    84901 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgtgg
    84961 tcgttttttc tcgctgttga tgatgcagga cagtgccagt gcggtcattg gcgcattgtt
    85021 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt
    85081 tgtgctatgt gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg
    85141 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    85201 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    85261 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    85321 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    85381 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    85441 cagcggccta caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    85501 cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc gcggcagcta
    85561 tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg
    85621 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    85681 aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgccgc
    85741 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    85801 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    85861 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    85921 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    85981 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    86041 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    86101 gcgcaaggtc aatggctacg tcaacaaact gctgttgccg cgttttgcca aatcggcatt
    86161 tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    86221 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    86281 tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    86341 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    86401 gccaagccgc gttgctaatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    86461 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    86521 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    86581 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    86641 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    86701 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    86761 gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt cgcgaagagc
    86821 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    86881 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    86941 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    87001 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    87061 aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg
    87121 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag
    87181 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    87241 ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca tcccaggtat
    87301 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    87361 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    87421 cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    87481 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    87541 atgagctgga gcgcggattt aatgaagccg tgttcaccgc tgcaaaactt tacccggcaa
    87601 acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg
    87661 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    87721 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc
    87781 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    87841 tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    87901 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac
    87961 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc
    88021 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg
    88081 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    88141 ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat ggcttgccgg
    88201 tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac
    88261 tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    88321 aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa taaccagtat
    88381 attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    88441 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    88501 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    88561 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    88621 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg atcgccccgg
    88681 cttttttatt ttacttacga attttgagta caaaatttct acacttcctc aaaataattg
    88741 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    88801 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    88861 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    88921 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    88981 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    89041 aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa ccactgatgt
    89101 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    89161 cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    89221 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    89281 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    89341 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    89401 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    89461 ttatggtgaa ggcgaaaagt atgtcccgtt ggtcctccgc gcgcaaacgc tgtgggatga
    89521 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
    89581 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
    89641 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
    89701 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    89761 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
    89821 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
    89881 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    89941 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
    90001 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    90061 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    90121 tgaacgtgtt ccgtttgcgg aagtggccag tgatgggtca gaagccttcc cgttcttgcg
    90181 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    90241 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
    90301 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    90361 tgcgcaagac caaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    90421 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    90481 ttgcactatc tcatcaataa tattcgcgag catctgatgc tttatctttt tctctgggga
    90541 ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc actgacataa
    90601 actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgag attattcctg
    90661 cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat gaagattctt
    90721 tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg ttatttttgt
    90781 gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta ttggatgcac
    90841 ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc ttccattggc
    90901 taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc acgctcagtt
    90961 attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt attttgttaa
    91021 tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc
    91081 cgagttattc gcccatgact cgtcttgccg caaaagctgg acatctcgca ttgtgcctat
    91141 tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt aaaccgatca
    91201 gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca caggcagact
    91261 ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtct gttatgcacg
    91321 gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag cgcatgctgg
    91381 gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct
    91441 tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa attgacaaag
    91501 aaggtcagca cgcctttgtt aacttccgca tacagcatct tggctatagc tggttatatg
    91561 gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatccg gctgccgata
    91621 aagtgaatgt gacaattaac accaccagcg tcgatactaa ccacgccgaa cgcgataaac
    91681 atcttcgcag tgcggatttt ctcaatacca caaaatatcc acaggcaaca ttcacctcca
    91741 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
    91801 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggcg
    91861 gtaaacgtgc aggctttgag gccgaaggca aaattaagct caaagacttc aatatcaaga
    91921 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
    91981 agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattctgttg
    92041 gatcaggaat acccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
    92101 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
    92161 agcaaatctc actacaacaa cctttgtatt tttccgcgca tactggacac tgaataaaca
    92221 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg ccgcactggt
    92281 ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca aaaacaaaat
    92341 ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg tattcaataa
    92401 ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag gcactggcct
    92461 tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta tgtgcctgca
    92521 tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tcgagcgcgt
    92581 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat agtgcatcgg
    92641 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca ttgctggcat
    92701 caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc atgcgcagta
    92761 cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc tctaaagccg
    92821 ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
    92881 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc gcggtaaaca
    92941 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
    93001 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg tcgttggaaa
    93061 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
    93121 cggcatgata tagcaattat cgataattaa catccacata ttttacgcta catttgcgca
    93181 ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag ccacaaattt
    93241 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgtataggtt gaaaaacagg
    93301 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
    93361 acctggttgg gtattggcgt actttggttg gtcgtgcaat tgccctaccc gattatctac
    93421 cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt
    93481 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
    93541 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggtat ggcgtggttc
    93601 tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
    93661 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctg
    93721 ggcgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
    93781 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
    93841 cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca
    93901 ccagatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc cgttgagcag
    93961 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
    94021 ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct gccaccagag
    94081 tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
    94141 aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt taaaacacgc
    94201 ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag aatggcatcc
    94261 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
    94321 ggaagtcggg cgcataatta gtgtgtttat cttttcttct tatgttcacc gcgcctggcg
    94381 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
    94441 aacctgatcg tcgcctggct aggctgtttt cttaccgggg ccgccttcag tctggtaatg
    94501 cccttcttac ccctctacgt tgagcagctt ggtgttaccg gccactccgc cctgaatatg
    94561 tggtccggta ttgtcttcag tataatgggt tcctaaacac atcattttag gtattgaata
    94621 tgagacgggc aatcagctac atcagatttt caagtgaacg acaactgaaa ggggactctg
    94681 tacgccgaca atctaagctg gttactgact ggcttgataa gaaccctgag ttttacttgg
    94741 attcatcctt gagctttaaa gatttgggga aaagtgcttt tagtggtaag cacctgaaag
    94801 gtggtctagg tgacttttta accgctatcg agaaagggct tgttaaagct ggtgatacgc
    94861 tactaatcga gagtttagac cgtctgtcac gtcaggatat tgatattgct tctgagctat
    94921 tacgccgtat cttaagggct ggtgtagatg tagtcacact gagcgatgga gaacattaca
    94981 cacgagaaag tttaaaagac ccgttagccc tgattaaaag cattctgata atgcagcggg
    95041 cgcatgagga aagtttgaga aagtctgagc gtgtccaagc tgcatggaac cgtaaaaaag
    95101 aactcatctc cgaaggtatc aaggtatcta ggcgttgtcc tgcatggtta aggctgaatg
    95161 atgaccgtcg aacattcaca atcattcctg ataaagtgga agttgttaaa cgagcatttg
    95221 acctgagatt acagggttta agcttttggg ctatcaccag aactttaaac gatgaaggtc
    95281 acttgtcatt gaatcaatac acacccaaac aaaaggggtg gtctgatact gctgtaaaga
    95341 aactgctcag aaatcgtgct gtgattggat gtttcacacc tgctggacgt gaagaagttc
    95401 agggttacta ccccgcaata atcag
//
LOCUS       DAJTUM010000003        75635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTUM010000003 DAJTUM010000000
VERSION     DAJTUM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(436..642)
                     /locus_tag="OO471_000237"
     CDS             complement(436..642)
                     /locus_tag="OO471_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6241651.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            955..1044
                     /gene="kdpF"
                     /locus_tag="OO471_000238"
     CDS             955..1044
                     /gene="kdpF"
                     /locus_tag="OO471_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6241652.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1044..2717
                     /gene="kdpA"
                     /locus_tag="OO471_000239"
     CDS             1044..2717
                     /gene="kdpA"
                     /locus_tag="OO471_000239"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6241653.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2740..4787
                     /gene="kdpB"
                     /locus_tag="OO471_000240"
                     /pseudo
     CDS             2740..4787
                     /gene="kdpB"
                     /locus_tag="OO471_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4796..5368
                     /gene="kdpC"
                     /locus_tag="OO471_000241"
     CDS             4796..5368
                     /gene="kdpC"
                     /locus_tag="OO471_000241"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6241654.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5361..8041
                     /gene="kdpD"
                     /locus_tag="OO471_000242"
                     /pseudo
     CDS             5361..8041
                     /gene="kdpD"
                     /locus_tag="OO471_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8038..8723
                     /gene="kdpE"
                     /locus_tag="OO471_000243"
                     /pseudo
     CDS             8038..8723
                     /gene="kdpE"
                     /locus_tag="OO471_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8998..9102
                     /locus_tag="OO471_000244"
     CDS             8998..9102
                     /locus_tag="OO471_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6241655.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9413..11610
                     /gene="speF"
                     /locus_tag="OO471_000245"
                     /pseudo
     CDS             9413..11610
                     /gene="speF"
                     /locus_tag="OO471_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11607..12926
                     /gene="potE"
                     /locus_tag="OO471_000246"
     CDS             11607..12926
                     /gene="potE"
                     /locus_tag="OO471_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6241656.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12982..14622)
                     /gene="pgm"
                     /locus_tag="OO471_000247"
     CDS             complement(12982..14622)
                     /gene="pgm"
                     /locus_tag="OO471_000247"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6241657.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14648..15193)
                     /gene="seqA"
                     /locus_tag="OO471_000248"
     CDS             complement(14648..15193)
                     /gene="seqA"
                     /locus_tag="OO471_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6241658.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15378..16142
                     /gene="ybfF"
                     /locus_tag="OO471_000249"
     CDS             15378..16142
                     /gene="ybfF"
                     /locus_tag="OO471_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6241659.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16282..16575
                     /gene="ybfE"
                     /locus_tag="OO471_000250"
     CDS             16282..16575
                     /gene="ybfE"
                     /locus_tag="OO471_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6241660.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16715..17245
                     /gene="fldA"
                     /locus_tag="OO471_000251"
     CDS             16715..17245
                     /gene="fldA"
                     /locus_tag="OO471_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6241661.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17455..17541
                     /gene="uof"
                     /locus_tag="OO471_000252"
     CDS             17455..17541
                     /gene="uof"
                     /locus_tag="OO471_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6241662.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17534..17980
                     /gene="fur"
                     /locus_tag="OO471_000253"
     CDS             17534..17980
                     /gene="fur"
                     /locus_tag="OO471_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6241663.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18064..18390)
                     /locus_tag="OO471_000254"
     CDS             complement(18064..18390)
                     /locus_tag="OO471_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241664.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18427..19833)
                     /gene="chiP"
                     /locus_tag="OO471_000255"
     CDS             complement(18427..19833)
                     /gene="chiP"
                     /locus_tag="OO471_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6241665.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20412..22076)
                     /gene="glnS"
                     /locus_tag="OO471_000256"
     CDS             complement(20412..22076)
                     /gene="glnS"
                     /locus_tag="OO471_000256"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6241666.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22279..24225)
                     /gene="nagE"
                     /locus_tag="OO471_000257"
     CDS             complement(22279..24225)
                     /gene="nagE"
                     /locus_tag="OO471_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6241667.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24558..25358
                     /gene="nagB"
                     /locus_tag="OO471_000258"
     CDS             24558..25358
                     /gene="nagB"
                     /locus_tag="OO471_000258"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6241668.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25418..26566
                     /gene="nagA"
                     /locus_tag="OO471_000259"
     CDS             25418..26566
                     /gene="nagA"
                     /locus_tag="OO471_000259"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6241669.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26575..27795
                     /gene="nagC"
                     /locus_tag="OO471_000260"
                     /gene_synonym="nagR"
     CDS             26575..27795
                     /gene="nagC"
                     /locus_tag="OO471_000260"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6241670.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27843..28595
                     /gene="nagD"
                     /locus_tag="OO471_000261"
                     /pseudo
     CDS             27843..28595
                     /gene="nagD"
                     /locus_tag="OO471_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28992..30656
                     /gene="asnB"
                     /locus_tag="OO471_000262"
     CDS             28992..30656
                     /gene="asnB"
                     /locus_tag="OO471_000262"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6241671.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31035..31111
                     /locus_tag="OO471_000263"
     tRNA            31035..31111
                     /locus_tag="OO471_000263"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31069..31071,aa:Met,seq:cat)
     gene            31120..31204
                     /locus_tag="OO471_000264"
     tRNA            31120..31204
                     /locus_tag="OO471_000264"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31154..31156,aa:Leu,seq:tag)
     gene            31228..31302
                     /locus_tag="OO471_000265"
     tRNA            31228..31302
                     /locus_tag="OO471_000265"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31260..31262,aa:Gln,seq:ttg)
     gene            31337..31411
                     /locus_tag="OO471_000266"
     tRNA            31337..31411
                     /locus_tag="OO471_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31369..31371,aa:Gln,seq:ttg)
     gene            31427..31503
                     /locus_tag="OO471_000267"
     tRNA            31427..31503
                     /locus_tag="OO471_000267"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31461..31463,aa:Met,seq:cat)
     gene            31552..31626
                     /locus_tag="OO471_000268"
     tRNA            31552..31626
                     /locus_tag="OO471_000268"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31584..31586,aa:Gln,seq:ctg)
     gene            31664..31738
                     /locus_tag="OO471_000269"
     tRNA            31664..31738
                     /locus_tag="OO471_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31696..31698,aa:Gln,seq:ctg)
     gene            complement(31892..33067)
                     /gene="ubiF"
                     /locus_tag="OO471_000270"
     CDS             complement(31892..33067)
                     /gene="ubiF"
                     /locus_tag="OO471_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6241672.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33213..34637
                     /gene="miaB"
                     /locus_tag="OO471_000271"
     CDS             33213..34637
                     /gene="miaB"
                     /locus_tag="OO471_000271"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6241673.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34790..35830
                     /locus_tag="OO471_000272"
     CDS             34790..35830
                     /locus_tag="OO471_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6241674.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35827..36294
                     /gene="ybeY"
                     /locus_tag="OO471_000273"
     CDS             35827..36294
                     /gene="ybeY"
                     /locus_tag="OO471_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6241675.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36384..37262
                     /gene="corC"
                     /locus_tag="OO471_000274"
     CDS             36384..37262
                     /gene="corC"
                     /locus_tag="OO471_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6241676.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37287..38825
                     /gene="lnt"
                     /locus_tag="OO471_000275"
     CDS             37287..38825
                     /gene="lnt"
                     /locus_tag="OO471_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6241677.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39222..40130
                     /gene="gltI"
                     /locus_tag="OO471_000276"
     CDS             39222..40130
                     /gene="gltI"
                     /locus_tag="OO471_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6241678.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40300..41040
                     /gene="gltJ"
                     /locus_tag="OO471_000277"
     CDS             40300..41040
                     /gene="gltJ"
                     /locus_tag="OO471_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6241679.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41040..41714
                     /gene="gltK"
                     /locus_tag="OO471_000278"
     CDS             41040..41714
                     /gene="gltK"
                     /locus_tag="OO471_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6241680.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41714..42439
                     /gene="gltL"
                     /locus_tag="OO471_000279"
     CDS             41714..42439
                     /gene="gltL"
                     /locus_tag="OO471_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6241681.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42557..43492
                     /gene="rihA"
                     /locus_tag="OO471_000280"
     CDS             42557..43492
                     /gene="rihA"
                     /locus_tag="OO471_000280"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6241682.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43576..45246
                     /gene="hscC"
                     /locus_tag="OO471_000281"
                     /pseudo
     CDS             43576..45246
                     /gene="hscC"
                     /locus_tag="OO471_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45306..46757)
                     /gene="djlC"
                     /locus_tag="OO471_000282"
     CDS             complement(45306..46757)
                     /gene="djlC"
                     /locus_tag="OO471_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6241683.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46754..47461)
                     /locus_tag="OO471_000283"
     CDS             complement(46754..47461)
                     /locus_tag="OO471_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6241684.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47563..48117
                     /locus_tag="OO471_000284"
     CDS             47563..48117
                     /locus_tag="OO471_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6241685.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48127..49554)
                     /locus_tag="OO471_000285"
     CDS             complement(48127..49554)
                     /locus_tag="OO471_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6241686.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49551..50258)
                     /locus_tag="OO471_000286"
     CDS             complement(49551..50258)
                     /locus_tag="OO471_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6241687.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50422..51399
                     /locus_tag="OO471_000287"
     CDS             50422..51399
                     /locus_tag="OO471_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6241688.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51469..51951)
                     /locus_tag="OO471_000288"
     CDS             complement(51469..51951)
                     /locus_tag="OO471_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6241689.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52186..54768
                     /gene="leuS"
                     /locus_tag="OO471_000289"
     CDS             52186..54768
                     /gene="leuS"
                     /locus_tag="OO471_000289"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6241690.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54783..55364
                     /gene="lptE"
                     /locus_tag="OO471_000290"
     CDS             54783..55364
                     /gene="lptE"
                     /locus_tag="OO471_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6241691.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55364..56395
                     /gene="holA"
                     /locus_tag="OO471_000291"
     CDS             55364..56395
                     /gene="holA"
                     /locus_tag="OO471_000291"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6241692.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56397..57038
                     /gene="nadD"
                     /locus_tag="OO471_000292"
     CDS             56397..57038
                     /gene="nadD"
                     /locus_tag="OO471_000292"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6241693.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57062..57673
                     /locus_tag="OO471_000293"
     CDS             57062..57673
                     /locus_tag="OO471_000293"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6241694.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57933..58250
                     /gene="rsfS"
                     /locus_tag="OO471_000294"
                     /gene_synonym="rsfA"
     CDS             57933..58250
                     /gene="rsfS"
                     /locus_tag="OO471_000294"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6241695.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58254..58721
                     /gene="rlmH"
                     /locus_tag="OO471_000295"
     CDS             58254..58721
                     /gene="rlmH"
                     /locus_tag="OO471_000295"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6241696.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58752..60653
                     /gene="mrdA"
                     /locus_tag="OO471_000296"
     CDS             58752..60653
                     /gene="mrdA"
                     /locus_tag="OO471_000296"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6241697.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60656..61768
                     /gene="mrdB"
                     /locus_tag="OO471_000297"
                     /gene_synonym="rodA"
     CDS             60656..61768
                     /gene="mrdB"
                     /locus_tag="OO471_000297"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6241698.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61779..62867
                     /gene="rlpA"
                     /locus_tag="OO471_000298"
     CDS             61779..62867
                     /gene="rlpA"
                     /locus_tag="OO471_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6241699.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63006..64217
                     /gene="dacA"
                     /locus_tag="OO471_000299"
     CDS             63006..64217
                     /gene="dacA"
                     /locus_tag="OO471_000299"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6241700.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64328..64591
                     /locus_tag="OO471_000300"
     CDS             64328..64591
                     /locus_tag="OO471_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6241701.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64692..65333
                     /gene="lipB"
                     /locus_tag="OO471_000301"
     CDS             64692..65333
                     /gene="lipB"
                     /locus_tag="OO471_000301"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6241702.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65592..66545
                     /locus_tag="OO471_000302"
     CDS             65592..66545
                     /locus_tag="OO471_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6241703.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66754..67719
                     /gene="lipA"
                     /locus_tag="OO471_000303"
     CDS             66754..67719
                     /gene="lipA"
                     /locus_tag="OO471_000303"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6241704.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67820..68023)
                     /gene="tatE"
                     /locus_tag="OO471_000304"
     CDS             complement(67820..68023)
                     /gene="tatE"
                     /locus_tag="OO471_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6241705.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68152..68940)
                     /locus_tag="OO471_000305"
     CDS             complement(68152..68940)
                     /locus_tag="OO471_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6241706.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69033..69416
                     /gene="crcB"
                     /locus_tag="OO471_000306"
     CDS             69033..69416
                     /gene="crcB"
                     /locus_tag="OO471_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6241707.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69470..69679)
                     /gene="cspE"
                     /locus_tag="OO471_000307"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69470..69679)
                     /gene="cspE"
                     /locus_tag="OO471_000307"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6241708.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69854..70414)
                     /gene="pagP"
                     /locus_tag="OO471_000308"
     CDS             complement(69854..70414)
                     /gene="pagP"
                     /locus_tag="OO471_000308"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6241709.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71003..72388
                     /gene="dcuC"
                     /locus_tag="OO471_000309"
     CDS             71003..72388
                     /gene="dcuC"
                     /locus_tag="OO471_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6241710.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72429..72770)
                     /locus_tag="OO471_000310"
                     /pseudo
     CDS             complement(72429..72770)
                     /locus_tag="OO471_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73108..73617)
                     /locus_tag="OO471_000311"
     CDS             complement(73108..73617)
                     /locus_tag="OO471_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6241711.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73780..74025
                     /locus_tag="OO471_000312"
     CDS             73780..74025
                     /locus_tag="OO471_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6241712.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            74000..75631
                     /locus_tag="OO471_000313"
                     /pseudo
     CDS             74000..75631
                     /locus_tag="OO471_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18614 a        18996 c        19210 g        18815 t
ORIGIN      
        1 acataacatc ttattggatt agatatgtta acaattaatt attcttaata tatcaataaa
       61 gtatatatta ctctctttac tatctgtttt tattgcggta gcgatactaa taacaaaatt
      121 cggaatttat ttagatgctg gaattagaaa aagggatgac ggctttctca ccttaccctg
      181 aagtttcacg aaagtgtgta aggaagtatg caatctaact ggagatttct gatgatctag
      241 tcgcttagag cgacaatgaa ggtgaccgca tcctgggtag ccctatgaaa ggatactatt
      301 gaaacgtgat gctaaaacag tgatgaaatg aataatacga tggaaacgtc ttccatgtta
      361 attatcatgg aagacgttga tgttgaacac ctaaaccagc cccctgatac gaggctggct
      421 taagcgcgca ggcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccacac
      481 cggtttatcg ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc
      541 tttccagaaa agcatgaaag gcagcgccag aacgcccgct gcaaccgcat atgtacggcg
      601 taaaaagata agccatgcag gatattctct gtagagttcc atacttttct cccccttttg
      661 tatctacccg gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg
      721 ccttttgtgt aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt
      781 acattcacac tgtaattaaa ttacacaaaa gttaaattaa tactaaccat cagttaaatc
      841 atggcctttg ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt
      901 tgcagccaga attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt
      961 gcaggcgtga tagccggcgt gttgctggtg tttttattac tgggctattt ggtttatgcc
     1021 ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt
     1081 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
     1141 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1201 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1261 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1321 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1381 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1441 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1501 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1561 tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag
     1621 gtgcgcagca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1681 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1741 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1801 tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag
     1861 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1921 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg
     1981 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta
     2041 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     2101 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     2161 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2221 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaata gacgtacgcg
     2281 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2341 cgttggcgat gatgaccgac gccggacgta gcgccatgct taaccctagc ccgcatggtt
     2401 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2461 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2521 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2581 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2641 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2701 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2761 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2821 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2881 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2941 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     3001 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc
     3061 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     3121 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3181 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3241 gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg
     3301 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3361 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3421 ctgaccatcg tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3481 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3541 ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3601 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3661 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc
     3721 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg
     3781 gaaggtcgca gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg
     3841 cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac
     3901 atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct
     3961 aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaagga
     4021 gccacgctgc tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat
     4081 atcgtcaaag gcggtattaa agagcgcttc gcccggctgc gcaaatgggc attaaaacgg
     4141 tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg
     4201 atgattttct cgccgaagcg acaccggagg ccaagctggc attgatccgt cagtatcagg
     4261 cggaaggtcg tttggtagcg atgaccggcg acagcaccaa cgatgctacg gcgctggcgc
     4321 aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggctaaagag gcgggcaata
     4381 tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga
     4441 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4501 tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg ctgaacatca
     4561 tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4621 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4681 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4741 gtatcaaagt cattgattta ctgctaaccg tttgtagtct ggtgtgaggt ttaccatgag
     4801 tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg gcggcgttta
     4861 cccgctgctg accaccgcac tggggcaatg gtggtatccc tggcaggcca acggttcgtt
     4921 gattcgtgaa ggtgatacgg tgcgcggttc ggcattaatc gggcaaaatt ttaccggcaa
     4981 cggctatttt catggtcgcc cgtcggcaac agcagaaatg ccctataatc cacaggcttc
     5041 tggcgggagc aatctggcag tcagtaaccc ggagctggat aaacagatag ccgcacgcgt
     5101 tgccgcatta cgggccgcta acccggacgc cagcacgaac gttccggttg aactggtgac
     5161 ggcgtcggca agcggactgg acaataacat caccccgcaa gcggcggcct ggcagatccc
     5221 acgcatcgca aaagcgcgta atctcagcgt tgaacagctc acgcagctga tcgcaaaata
     5281 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa
     5341 tctggcactg gataagcttg atgaataacg aacccttacg tcccgacccc gaccgtctgc
     5401 tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcagt gcctgtgcag
     5461 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5521 atattgtggt tggtgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5581 ggctggctgt tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5641 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5701 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5761 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5821 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5881 ccgacgacgt ggtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5941 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     6001 atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa
     6061 tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc
     6121 ttttatgcat cggccataac accggcagcg aaaactggtc cgcgcggcgg cgcggctgac
     6181 gtcacggctg gggagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt
     6241 accggagaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc
     6301 ggagacggca acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga
     6361 acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga
     6421 aacatttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     6481 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctcat ttaaagacaa
     6541 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt
     6601 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct
     6661 tggcgtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa
     6721 tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt
     6781 gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac
     6841 tgctggtgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt
     6901 atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag
     6961 cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa
     7021 cggtaaattg cagccgttaa cacatccgca aggaattacg ccatgggacg atgccatcgc
     7081 gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt
     7141 accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga
     7201 gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacttttac
     7261 gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg
     7321 gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt
     7381 acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag
     7441 cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac
     7501 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
     7561 gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc cggaaccggg
     7621 tttattgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
     7681 gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc
     7741 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
     7801 ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg
     7861 cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat
     7921 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
     7981 tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg
     8041 acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg
     8101 gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg
     8161 gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
     8221 gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc
     8281 agcgaagaga gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag
     8341 ccgtttggta ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc
     8401 actccgccac cgccgcgccc gatccgctgg tgaaattttc cggtgttacc gtcgatttag
     8461 ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc
     8521 tgctggcggt gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc
     8581 aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc
     8641 tgcgacaaaa actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta
     8701 ttgggtatcg gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat
     8761 ttattattaa acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata
     8821 taaaacacga aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata
     8881 attaatgatt tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag
     8941 ccatttcatg gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg
     9001 gaaaataata accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc
     9061 catcgtaata gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca
     9121 ccccaacgta caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt
     9181 gctcatatac tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt
     9241 gaggtatgac gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc
     9301 aaactactga tttatttatc agcggtggag ctttgctttt tttccgacgt gatcgatttc
     9361 tcctttgaga aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga
     9421 attaaaaatt gccgttagcc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt
     9481 gaatattgac gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt
     9541 tgaacgtgga aaactcgata aaatagacgc tactggctat ggcattcctg tttttattgc
     9601 aacggaaaat gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga
     9661 gcattgcgaa tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta
     9721 tgaaacgcaa ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa
     9781 tagcgctttt gactgcccgg gtcatcaggg aggtgagttt ttccgtcgtc atccggcagg
     9841 gaatcagtcc gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga
     9901 cgtagcaatg ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc
     9961 ggcaaaagtg tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa
    10021 caaagtggtt ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa
    10081 ccacaaatct aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga
    10141 aacggcacgt aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag
    10201 ttacctgcgt gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt
    10261 ccgcctcgct gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt
    10321 ggtggataaa attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta
    10381 tgagcaattt atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa
    10441 cgatccgggt attctagtta cgcaatccgt acataaacag caggctggtt tttctcaaac
    10501 ttcacaaatc cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa
    10561 acgcatgaac aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc
    10621 cgcactggat atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga
    10681 ttgtgtggta aatggcatta atgcccgcaa actgatcctc gataactgtc agcatattcg
    10741 tccgttcgta cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat
    10801 tgcggttgat ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg
    10861 ctatgcagag aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat
    10921 tgatgcacgt aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt
    10981 cctgcgcgaa aatggcgtag tgccggaaaa atgcgatctt aacgccatcc tcttcctgct
    11041 gactccggca gaagatatgg ccaaacttca gcaacttgtg ccctgctggt acgcttcgaa
    11101 aaactgcttg agtccgacgc gccattagca gaagtgctgc cttccatcta caaacagcat
    11161 gaagagcgct acgccggtta taccctgcgt cagttgtgcc aggaaatgca tgatttgtat
    11221 gcccgccaca acgtgaaaca actgcaaaaa gagatgttcc gcaaagcgca cttcccacac
    11281 gtcagcatga atccgcaaga agccaactac gcctatttac gcggtgaagt ggagctggtt
    11341 cgtctaccgg atgccgaagg tcgtatcgct gccgaagggg cgcttcctta tcctccgggt
    11401 gtgctgtgtg ttgttccggg tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct
    11461 ctggaagaag ggatcaacct gctgccaggt tttgcaccgg agctgcaggg tgtctatatc
    11521 gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa
    11581 agcaccctgt tgaaagggga aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg
    11641 ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga
    11701 caaagcttgc cgaagttggg acaatttcaa ttatctcctg gctggtgaca gccgtaggct
    11761 caatggcacc ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta
    11821 tgggcggtta tgccgagtat gctttcggta aatccggtaa ctttattgcg aactatacct
    11881 acggcgtctc actgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca
    11941 ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc
    12001 tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca
    12061 ttaccgtgtg ggggggcatt attccggtgg taggcctgtg cattatcggc tggttctggt
    12121 ttagcccgac gctttacgtt gattcctgga atccgcatca tgcaccgttc ttcagtgcag
    12181 taggttcttc catcgctatg acgctgtggg cttttcttgg tctggagtct gcgtgtgcga
    12241 atactgatgt agtggaaaac ccggaacgta atgtgccaat cgcggtactc ggcggtacgt
    12301 taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgcagggatt gtgcctaata
    12361 tggagctggc aaattcaacg gcaccatttg gcctggcctt cgcacagatg tttacaccag
    12421 aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct
    12481 ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa
    12541 ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta
    12601 tccagagcgg gttggcgctg atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc
    12661 tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag
    12721 tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca aactttgttg
    12781 cttttgttgg cgcgatgtat agcttttatg cgctgtactc atccggggaa gaagccatgc
    12841 tgtacggttc catcgtgacc ttccttggtt ggacactgta tggtctggta tcaccacgct
    12901 ttgaactgaa aaataaacac ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg
    12961 ctcttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc
    13021 ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt agatcttata
    13081 tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca tcactttcag
    13141 accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga tcgggtcacc
    13201 tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg ctttttgtgc
    13261 ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt ttgccagttc
    13321 gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca
    13381 cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc
    13441 ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat caacaaacca
    13501 tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca cacggtcgat
    13561 catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg
    13621 ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg
    13681 agtgacgata ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc
    13741 acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac ggatcgcacc
    13801 gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag
    13861 gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac cgcccaacgg
    13921 atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga tatcggtcag
    13981 cccttccacg aacggctgca ccagatcctg ctctttcaca tagccggatg ccatcgcttc
    14041 gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt tggccttgtc
    14101 ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat tgtacttgat
    14161 accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc
    14221 acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag tgaagccatt
    14281 gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta cggaaatgaa
    14341 cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag tgatgccgtt
    14401 tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct
    14461 gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt
    14521 ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc
    14581 actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg
    14641 caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat tgcatcgact
    14701 gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat
    14761 acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt
    14821 catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg
    14881 cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct
    14941 tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa
    15001 tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca
    15061 ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa atgtcggatg
    15121 cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt
    15181 caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaagagtat
    15241 atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa
    15301 ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg
    15361 aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa aaccagcaca
    15421 ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac cttggcgtac
    15481 tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc
    15541 tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt gttgataccc
    15601 tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg
    15661 taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg atcgatatcg
    15721 caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc aacgcggtca
    15781 gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag catcttaatg
    15841 aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg
    15901 tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg
    15961 atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg
    16021 atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc gcgggtcact
    16081 gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt
    16141 aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga
    16201 ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg gctgattgtt
    16261 tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg
    16321 ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgaacag
    16381 ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc
    16441 gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga atcgcgtaat
    16501 atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt
    16561 cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct
    16621 gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc
    16681 ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg
    16741 cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga
    16801 cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag cttacgacat
    16861 tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt
    16921 cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt ttggttgtgg
    16981 tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc gcgacatcat
    17041 tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc atttcgaagc
    17101 atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg aagaccgtca
    17161 gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg aagagttgca
    17221 tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt
    17281 aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctat
    17341 acaatgtccc tgtgttcaag tggccttgcc gttgtaaatg taagctgtgc cacgttttta
    17401 ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata
    17461 cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca
    17521 ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac
    17581 gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc
    17641 ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata
    17701 tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg
    17761 taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt gcctcgactg
    17821 cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc
    17881 aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg
    17941 cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag
    18001 ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag ttacagctct
    18061 cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg ctttcaccgc
    18121 ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta cgcgtacact
    18181 ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta acacgctctg
    18241 gcaggcttca attttttctg gcgaaccttg cgcggtgttg atacaggcgc tgtatgcctc
    18301 tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca gccccgatgc
    18361 catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa cctgccgcgt
    18421 cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac gctcatcctg
    18481 gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt cgtattcggt
    18541 gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt agaccgcatc
    18601 caggctgtag gcagactctt caatagtccg gtttttgtcg tagtacgcat ccggattgct
    18661 ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga tggcgaagcc
    18721 tggaagattc cagtttttca ggtcatacat cgcactgaag aagaccgctt tttcgccgtt
    18781 agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg aggaagcgta
    18841 ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa tccaggtgcc
    18901 ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca
    18961 ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc gggtaccata
    19021 gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc
    19081 aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta cgaagttatt
    19141 tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg ttttatcgtt
    19201 ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg tccacatgta
    19261 ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt ccgccccctg
    19321 ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg ttggctgaat
    19381 gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt tatacaggct
    19441 tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc
    19501 gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcagc
    19561 aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt tggcattcca
    19621 ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg
    19681 ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga atccttcggc
    19741 gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa tagccagcgc
    19801 cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt
    19861 gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    19921 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    19981 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    20041 agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga aattccagca agatgaaagc
    20101 ggttacattt tatttaatta tgttttgtga ttttaaattt attttatgga taaattgcga
    20161 agcggaatta attcgcgaag aaaagcagaa aaaacgccgc agagcgcggc gtttgggatt
    20221 gtttgctatt tcttgccgga tgcggcgtga acgccttatc cggcctacaa aagcacgcaa
    20281 attcaatata ttgcagagat catgtaggcc tgataagcga agcgcatcag gcaatttagc
    20341 gtttgaaatt tattgtctat ttattgccgg atgcgacgta aatgcctcac ccggcttggt
    20401 agaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta
    20461 aataccggtt tttcggccgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca
    20521 aactggaatg ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg
    20581 atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc tgggttaggc
    20641 acgctgaaca gacgatcata caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc
    20701 cagtgaataa cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg
    20761 tcataagtac agaagatggt ggtgatatta ccttcggcat ctttctccac gcgttcagcc
    20821 ttaatgacgt aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc
    20881 ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa tttcaccgct aaacggcacc
    20941 tgacggctcc ccatttccgg tttgttcgga tggttcggca tggtgaccat ttcgccttcg
    21001 ccctgatagt tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca
    21061 ttttcgttga gatcttcacg gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc
    21121 tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagccgcagt gtaaccacga
    21181 cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg
    21241 gtcaccagca ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag
    21301 aattcgtact ggcgcggatg aacaggaatg gtgatgttat ccagtaccca gtcgtacaga
    21361 cgacggttgt cctggaactc aagcgtacac agagagtgcg taataccttc cagcgcatcg
    21421 ctgatgcaat gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg
    21481 tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca ttacgataaa tggcgacgcc
    21541 atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt
    21601 tcgaacagcg ccaagttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggc
    21661 tgcgtcaggg taccgcggta ttcgcggatc tgttccggtg tcagttcatc aacgtacgcc
    21721 aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag
    21781 taacggacgt taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgaatcg
    21841 acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct
    21901 ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga
    21961 tagccattcg gctccggcgg gaaacgggtg tgtactgtga tgtgcttacc actggccaga
    22021 tcttcatcga tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg
    22081 gattcctcaa agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga
    22141 caactgttag ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa
    22201 aaaaagcggc agagaagatc tccaccgctt gtcattgttg gatgcgacgc tgccgtgtcg
    22261 catcaggcat aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac
    22321 atggccctga gctttaatga gcaagccact gaaatcgtcg atattgctgc aaaccaccgg
    22381 gctaatcatc gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg
    22441 ccctgcgctt acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc
    22501 tacggtgtcg atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa
    22561 cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt
    22621 atctgtcggt ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg
    22681 aacctgatcc agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac
    22741 cgcgtttggt acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc
    22801 tgacgcagcg gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc
    22861 tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc
    22921 acccagacgt ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag
    22981 acgggtaata caggcatcaa tcgctttcag gttgtcagta ccgccaaccg cagcaatata
    23041 gttggttgcc agttgagtca gaccttcttc agtattgctg ttggcttctt cagtaacgat
    23101 ctcgtcttct ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact
    23161 gaacaccacg aagtagatag cgaagaagat aacgcccatc accagcagca tccagacgtt
    23221 ctggctggcg gcaggcaggt tatacatcaa cgcgtagtcg atagcacccg cagagaaaga
    23281 gaagccggca tggataccca gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc
    23341 gtgcaggagg tacagcagcg gcgcaaggaa catgaacagg aattccagcg gctcagtcac
    23401 accggtcagg aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg
    23461 ctctttcggt gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcatgat
    23521 cgggaagaag ccggacatga acatccccgc ggtgccgtca ccagcgtaga aacggttgat
    23581 gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat
    23641 ggtgttcagc acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat
    23701 accggaaccc agcgcgcccg cagaaacgat ccactcgccg cctgcatgga tagcgtgctg
    23761 taccggcggc cagacgtaac caaaaatggc cgccagcacc agacagaaga agccggtggc
    23821 gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt taatatcgga
    23881 ccaacggtta taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat
    23941 gttaatttct gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc
    24001 cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa
    24061 gattaatgcg aggttatcaa aaattgcacc gcccgcctgg gcaataaacg caacgttaag
    24121 taaatctggc tgaccgaatc gcagcaacag cgccgccacc ggcagcaccg cgatagggag
    24181 ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg
    24241 agaaccctat ttggctcgtt taaagccgta tttttatttt gctgcaaatt ttactgccga
    24301 tgttctgtaa tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgcat
    24361 cgcaaattaa acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc
    24421 ttttactggc taaaccagaa aacttatttt atcattcaaa aaatcaagtc ggattgacgc
    24481 ctgtctgcgc aaatccaggt tacgcttaaa gatgtctaat ccgccaacgg cttacatttt
    24541 acttattgag gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat
    24601 gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa accaactgcc gatcgtccgt
    24661 ttgtactggg cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa
    24721 tgcataaagc aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg
    24781 tcggtctgcc gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg
    24841 atcacgttga tattccagca gaaaacatca accttctcaa cggcaatgcc ccggatatcg
    24901 atgccgagtg ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta
    24961 tgggcggtgt aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt
    25021 ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt cgcgaactct cgtttctttg
    25081 ataacgatgt caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acactgctgg
    25141 atgccgaaga agtgatgatt atggtgctgg gtagccagaa agcactggcg ctgcaggccg
    25201 ccgttgaagg ttgcgtgaac catatgtgga ccatcagctg tctgcaactg catccgaaag
    25261 cgatcatggt gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt
    25321 tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggttcc
    25381 ttgctcaggg caatattttt taaaatcggg gggcagaatg tatgcattaa cccagggccg
    25441 gatctttacc ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat
    25501 taaaagcgtc tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg
    25561 ggccattctc tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt
    25621 taacgacacc gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga
    25681 gaaatcaggc tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa
    25741 acagggcgtg cgcgttatgc gtgagtacct ggcaaaacat ccgaatcagg cgttaggtct
    25801 gcatctggaa ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt
    25861 gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa
    25921 agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg
    25981 gattgtggtt tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg
    26041 cgcggggatt acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga
    26101 accgggcctg gcgggcgcga tcctcgacga agctgacatt tattgcggta ttatcgctga
    26161 tggcctgcat gttgattacg ccaacattcg taacgctaaa cgccttaaag gcgacaaact
    26221 gtgtctggtt accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc
    26281 gggtaaaaca atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg
    26341 ttcatcctta accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact
    26401 ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt gcgattgacg ttgaaaaacg
    26461 tctcggcacg ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa
    26521 aatcaccaag accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca
    26581 ccaggcggac aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg
    26641 gtttatcgcc tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc
    26701 cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc
    26761 aaagaagttg atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa
    26821 acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc atgacgccac catcactctg
    26881 tttgatctca gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag
    26941 cagacgctgg aacatgccct gctgaatgcc attgctcagt ttattgatag ctaccagcgc
    27001 aagctgcgcg agctaatcgc catttcggtg atcctgccag ggcttgttga cccggacagc
    27061 ggcaaaattc attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg
    27121 gaagaacgtt ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg
    27181 gagcactact tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc
    27241 ggaaccgggg ccgggattat ctctaacggg cgcattttta ttggccgcaa cggtaacgtc
    27301 ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt
    27361 ggctgcctgg aaactatcgc cgccaacgct gccattgaac aacgggtgtt gaatctgtta
    27421 aagcagggct accagagccg tgtgccactg gacgactgca ccatcaaaac tatctgcaaa
    27481 gccgcgaaca aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg
    27541 ggtaaaacca tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgctggt
    27601 gaaatcaccg aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccaa
    27661 gcgctgaagg cgtttcgtac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg
    27721 gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat
    27781 ttgctggaaa attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt
    27841 ccatgaccat taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg
    27901 ccgtaccggg tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt
    27961 tgctgaccaa ctatccttcg cagactgggc aagatctggc gaaccgcttt gccactgccg
    28021 gtgtcgatgt gccggatagc gtgttttata cctctgcgat ggcgaccgcc gatttcctgc
    28081 gtcgctagga aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt
    28141 acaaagccgg tttcactatt accgatgtga accctgattt cgtgattgtt ggcgaaacac
    28201 gttcctacaa ctgggacatg attcataaag cagcctattt cgtcgctaac ggtgcacgct
    28261 ttatcgccac caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt
    28321 gtgcagggat tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt
    28381 ggatcatccg cgcggcatta aacaaaatgc aggcgcattc ggaagaaaca gtgattgtcg
    28441 gcgataacct gcgtaccgat attctggcgg gcttccaggc cggtctggag acgattctgg
    28501 tgctttctgg tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga
    28561 tttacccgtc agtcgctgaa atcgacgtta tctgaatacc acccacgtct gcggacgtgg
    28621 ttggctctga tgacaaacgc caaactgcct gatgcgctac gcttatcagg cctacgcagc
    28681 tcctgcaata tattgaattt tcatgctttt gtaggccgga taaggcgttc acgccgcatc
    28741 cggcatgaac aaatcgcact ttatcagcaa tttggccccc gtaaaatata accattcaat
    28801 aaaacccgct aaaaattatc gattcatcaa taaatacatc ctcacaccag accctttttc
    28861 accaatcgac aatcaccatt acgtttttta tttttgcact gccaaatagc ttgcgcacag
    28921 tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat accaggttaa
    28981 cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac gcagttgagc
    29041 tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg
    29101 gtatttatgc cagcgataac gccattctcg ctcacgaacg tctgtcaatt gttgacgtta
    29161 acgcgggggc gcaacctctc tacaaccaac aaaaaaacca cgtactggcg gtaaacggtg
    29221 aaatctacaa ccaccaggca ttgcgcgctg aatatggcga tcgttatcag ttccagacag
    29281 ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg
    29341 acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgcc tacctgattg
    29401 gtcgcgacca tctggggatc atcccactgt atatggggta tgacgaacac ggtcagctgt
    29461 atgtggcctc agaaatgaaa tcgctggtgc cagtttgccg cacgattaaa gagttcccgg
    29521 cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat cgcgactggt
    29581 tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt caggcactgg
    29641 aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgcta ctttctggtg
    29701 gtctggattc ctcaattatt tccgctatca ccaagaaata cgcagcccgt cgcgtggaag
    29761 atcaggaacg ctctgaagcc tggtggccgc agttacactc ctttgctgta ggtctgccgg
    29821 gttcaccgga tcttaaagca gcccaggaag tggcaaacca tctgggcacg gtgcatcacg
    29881 aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt taccacatcg
    29941 aaacttatga tgtgaccact attcgcgctt caacaccgat gtatttaatg tcgcgtaaga
    30001 tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa gtgtttggcg
    30061 gttatcttta cttccataaa gcgcccaacg ttaaagaact gcatgaagag acggtgcgta
    30121 aactgctggc cttgcatatg tatgactgcg cgcgcgccaa caaagcgatg tcagcctggg
    30181 gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg atgcgcatta
    30241 acccgcagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt
    30301 ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc tccgatggcg
    30361 tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt tctgatcagc
    30421 aactggaaac tgcccgcttc cgcttcccgt acaacacgcc gacctcaaaa gaagcgtatc
    30481 tgtaccgcga gatctttgaa gaactgttcc cacttccgag tgccgctgag tgcgtgccgg
    30541 gcggtccttc cgtcgcgtgt tcttcagcta aagcgatcga atgggatgaa gcgttcaaaa
    30601 aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat aagtaagaaa
    30661 tgcggtactg ccccggagaa atccggggct gttttagtta ttgcgctgct tgttgattct
    30721 gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac tcatcactgt
    30781 aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa tttcatcctg
    30841 tacgacaaaa gaatggctgt agggtgggga aaaacacaaa gtaaacaata attgacgaat
    30901 atagcgccac gctgttcgca acctaaccaa acagtcactt tcgagcaatt ttccttgaaa
    30961 aagaggttga cgctgcaagg ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat
    31021 cgcgaaaaaa agatggctac gtagctcagt tggttagagc acatcactca taatgatggg
    31081 gtcacaggtt cgaatcccgt cgtagccacc atcttttttg cgggagtggc gaaattggta
    31141 gacgcaccag atttaggttc tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc
    31201 accattcacc agaaagcgtt gtacggatgg ggtatcgcca agcggtaagg caccggtttt
    31261 tgataccggc attccctggt tcgaatccag gtaccccagc catcttcttc gagtaagcgg
    31321 ttcaccgccc ggttattggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    31381 ttccctggtt cgaatccagg taccccagcc atcgaagaaa taatctggct acgtagctca
    31441 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    31501 ccaaattctg aatgtgtcga atacgttcgg caaattcaaa aaccaatttg ttggggtatc
    31561 gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc
    31621 cagccaattt attcaagacg cttacatcgt aagtgcaccc agttggggta tcgccaagcg
    31681 gtaaggcacc ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaca
    31741 ttaaaaaagc tcgcttcggc gagctttttg cttttctgcg ttcattcaat gtcgaatgcg
    31801 atgttgacac gtcttatcct tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta
    31861 cggctggcac gcatccggca atgttgtaag gctacaaccc taacgcatat ttcagcgcct
    31921 gacgtttcaa cacgccagca cgctctgccg ccattaaccc gagattacgc acaaaacgca
    31981 gcggcggcag attattactg aatccggcat aaaacagatc cataccactt tgcataatga
    32041 agttatccgc catgcgccgc atctggtaac gcttgaggac aggataactg gcccacgctt
    32101 caccgtagct gcgggcattc accagaacat caatcagggc atcgacatca cgataaccaa
    32161 gattcacccc ctgccccgcc agcggatgga tggtatgcgc ggcatcgccc accagcgcaa
    32221 gccccggctg cacatactgc aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa
    32281 gcggtgtaac gtaacccaga cgcgacggga aatgcttcgc gatttccgcc tggagttgcg
    32341 ccatattcat attctgcaac tggcgaatac gcgtcggaga gtcataccac accagcgatg
    32401 cccagttatc aaacaacggc agaaacgcac gcggtccgtc cggggtaaat tgctgccagg
    32461 tgctgtcgcc gggatcgttc tcacactgta cgctaatcaa catgcacgac tgcgcatact
    32521 gccaggcatg aacgccaatt cccgccatct gccgcacctg agaatttgcg ccgtcggcac
    32581 caatcaccag tttcgcgcgg atcgtttcac cgcccttcag ttccagctcc tgaagatcat
    32641 tatctcgatg taatgcaatc agcgaggttg gcacacgtaa cgttactttc ggatgcgctt
    32701 ccagcgcctg ccacaacgcc tgttgcagga ctgtgttttc caccatgtag ccaagcaacg
    32761 gcaactttaa ttcggctgcg tcaaatacca catgtgccgt ttcccactcc caggtttcca
    32821 gtctgcggta aggatggcaa cgcatagcct gtactgcatc ccagacccct aaccctttaa
    32881 gcaatgatac cgaagccgcg ctgatcgccg agatccgcac gtccggttgg ctgtcagcga
    32941 caaacggtgc aggttctgcg tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc
    33001 ccagcgccag tgcgccgccg accattcctc cgccgacaat ggcaatttcc gttggttgat
    33061 ttgtcatggt caatcatcct gttagcaata tgctttaagt ttacaggatt tttaccccca
    33121 agggctgata acgctcatac tggtcacaac ggcagcaaag cattacacta tgcgccctgc
    33181 attcctggct actatttcgc aagagcaagt cgatgaccaa aaaactccat attaaaacct
    33241 ggggctgtca gatgaacgag tacgattcat cgaagatggc cgatctgctg gatgcctccc
    33301 acggctatca actgaccgac gtggcggaag aagcggatgt gctactgctg aacacctgct
    33361 caatccgcga gaaggctcag gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa
    33421 aagagaagaa tccagacctg attatcggcg tcggtggctg cgtggcatcg caagaaggcg
    33481 agcacattcg ccagcgcgcc cactatgtcg atattatttt tgggccgcaa acgctgcacc
    33541 gtctgccgga gatgatcaac tccgtgcgcg gcgaccgcag cccggttgta gatatcagct
    33601 tcccggaaat cgagaagttt gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt
    33661 ttgtctccat catggaaggc tgcaataaat attgcaccta ctgcgtggtg ccttacaccc
    33721 gtggtgaaga ggtaagccgt ccgtctgacg atattctgtt tgagattgcc cagcttgcgg
    33781 ctcagggcgt gcgtgaagtc aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga
    33841 actacgacgg caccaccgga tcgtttgccg atctgttgcg tctggttgct gcgatcgacg
    33901 ggatcgatcg tattcgcttt accaccagcc atccgatcga attcaccgac gatatcatcg
    33961 aagtgtaccg cgacacgcct gagctggtga gcttcctgca tctgccggta cagagcggtt
    34021 ccgatcgcat tctgaacctg atgggacgta cccatacggc gctggagtac aaagcgatca
    34081 tccgtaaact gcgtgcggcg cgtccggata ttcagatcag ttctgacttc attgttggct
    34141 tccctggcga aaccaccgaa gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt
    34201 tcgacatgag ctacagcttt atcttctctg cacgtccagg aacgcctgcc gccgatatgg
    34261 ttgatgatgt tccggaagaa gagaagaagc agcgtctgta tattctgcaa gaacgtatca
    34321 atcagcaagc gatggcatgg agccgtcgga tgctcggcac cacccagcgt attctggtag
    34381 aagggacttc gcgtaagagc atcatggagc tttccgggcg taccgaaaat aaccgcgtgg
    34441 tcaacttcga gggcacaccg gatatgatcg gtaaattcgt cgatgtagaa attaccgacg
    34501 tctacccgaa ctctctgcgc ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgca
    34561 tggcagaaac accggagtcg gtgattgccc gcacccgcaa agaaaacgac cttggcgtgg
    34621 gttattatca gccgtaattc tcaggcctgc cgttccggtg ggccttgttt tttctctctt
    34681 tatccccatc tttatcgcaa tgcttgcgcc tttttcctgt ggcgtgaata atttccttat
    34741 aggccggtga atgttcagct tccggcggca tacagataag aggaacggtt tgaacataga
    34801 cactcgcgaa atcaccctgg agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc
    34861 gtttgatgac aacatcaagc agctcgaacg ccgtctcggc atcgagatca atcgccgcga
    34921 taaccacttt aaactgaccg gccgtccgat ttgcgtcacc gctgcggcag acattctgcg
    34981 tagcctgtat gtcgatactg ccccgatgcg cggtcagatt caggatatcg aaccggaaca
    35041 gatccacctt gcgattaaag aagcgcgggt actggagcaa agcgcggaga gcgtgccgga
    35101 gtacggcaaa gcggtcaata tcaaaactaa acgcggcgta attaagccgc gcacgccaaa
    35161 ccaggcgcag tacatcgcca atattctcga ccatgacatt accttcggcg ttggcccggc
    35221 gggtacgggt aaaacctacc tggcagtggc tgcggcagtt gatgccctgg agcgtcagga
    35281 aattcgccgt attctgctga ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct
    35341 gcctggcgat ttaagccaga aagtagaccc gtatctgcgc ccactgtacg acgcgctgtt
    35401 tgaaatgctg ggctttgaga aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc
    35461 gccgctggcc tatatgcgtg gtcgtacgct gaacgacgcg tttatcattc tcgatgagag
    35521 ccagaacact accatcgaac agatgaagat gttcctgacc cgtatcggtt ttaactcaaa
    35581 agcggttatc accggcgacg tcacacagat cgacctgccg cgtaatacta aatcaggctt
    35641 acgtcatgcc attgaagtgc tggccgatgt cgaagagatc agctttaact tcttccacag
    35701 cgaagacgtg gttcgtcacc cggtggtggc gcgtatcgtt aacgcttatg aagcctggga
    35761 agaagcagaa caaaaacgta aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga
    35821 acaaaaatga gtcaggtgat cctcgattta caactggcat gtgaagataa ttccgggtta
    35881 ccggaagaga gccagtttca aacatggctg aatgcggtga tcccgcagtt tcaggaagag
    35941 tcggaagtga ctattcgcgt ggtcgatacc gccgaaagcc acagtctgaa tctgacctat
    36001 cgcggtaagg ataagccgac caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg
    36061 gagatgtcgc tactgggcga tctggttatc tgccgtcagg tggttgagaa ggaagctcag
    36121 gagcaaggca aaccactgga ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat
    36181 ttgttaggtt acgatcacat tgaagatgac gaagcagaag aaatggaagc cctcgaaaca
    36241 gagattatgc ttgctctggg ctatgaggat ccgtacattg ccgagaaaga ataatttgcc
    36301 aggcatttga ctggcagacg tccccgccgt tggtgattaa cgacggcgtt ttgattaact
    36361 aacatgagaa cccatacgac gccatgagcg acgacaattc acacagtagt gacacgataa
    36421 gcaacaagaa gggatttttc tccctgttac tcagccaact tttccacggt gaaccgaaaa
    36481 accgtgacga actgctggcg ctgatccgtg attccgggca gaacgacctt atcgacgaag
    36541 atacgcgcga tatgctcgaa ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca
    36601 tgatcccccg ctcccagatg attaccctga aacgcaacca gacgctggac gaatgccttg
    36661 atgtcatcat cgagtccgcc cactcacgtt tcccggtgat tagcgaagac aaagatcaca
    36721 ttgaagggat tctgatggcg aaagacttgc tgccgtttat gcgcagcgat gctgaagcct
    36781 tcagcatgga caaagtgtta cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc
    36841 ggatgctgaa agagtttcgc tctcagcgtt accacatggc gatcgttatt gacgaattcg
    36901 gtggggtttc cggtctggtg accattgaag acatcctgga actgattgtt ggtgagattg
    36961 aagacgagta tgacgaagaa gatgatatcg acttccgtca gctgagtcgt catacctgga
    37021 ccgtgcgcgc actggcttcc attgaagact tcaacgaagc gttcggcacc cactttagcg
    37081 atgaagaagt cgacactatc ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc
    37141 gtggcgaaac tatcgacatc gacggttacc agttcaaagt ggcgatggcc gatagtcggc
    37201 gtattattca ggttcatgtc aaaatcccgg atgactcacc ccagccaaag ctggatgaat
    37261 aaaaccgaaa ctggatagat aactacatgg attttgcctc attaattgaa cgccagcgca
    37321 ttcgcctgct gctggcgtta ttattcggtg cctgcggaac gctggccttc tctccttacg
    37381 acgtctggcc tgcggcgatt atttcgctga tggggcttca ggcgttgacc tttaaccgtc
    37441 gcccactcca gtctgccgct attggctttt gctggggatt tggcctcttt ggcagcggta
    37501 ttaactgggt ctatgtcagc atcgcgacct ttggcgggat gcctggcccg gttaacatct
    37561 tcctggtggt gctgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc
    37621 tgtcgcgtct gtggccgaaa accacctggc tgcgcgtggc gattgccacc cctgctctct
    37681 ggcaagtgac cgagtttctg cgcggttggg tgctgactgg cttcccgtgg ttacagttcg
    37741 gctatagcca gattgatggc ccgttaaaag ggctggcacc gctaatgggc gtggaagcca
    37801 ttaacttcct gctgatgatg gttagtggcc tgctggcact ggcgttggtc aaacgcaact
    37861 ggcgtccgct ggtggtggcc gtcgtgctgt ttgccctacc cttcccgctg cgttacatcc
    37921 aatggtttac cccgcaaccg gagaaaacca ttcaggtttc gatggttcag ggcgatattc
    37981 cgcaatcgct gaaatgggac gaaggccagc ttcttaatac gctaaagatt tactacaacg
    38041 caacggcacc gctgatgggc aaatcatcgt tgattatctg gccggagtcg gcgataaccg
    38101 atctggaaat taatcagcaa ccgttcctca aagcactgga cggtgagttg cgtgataaag
    38161 gtagctcgct ggtaaccggg attgtcgacg cgcgtctcaa taagcagaac cgctacgata
    38221 cctacaacac catcatcacg ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc
    38281 gctataacaa aaaccatctg gtgccgtttg gcgagtttgt cccgctggag tcgattctgc
    38341 gtccgttagc accgttcttt gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc
    38401 agccgccgct gtcggcaaat ggtattgagc ttactgcggc tatttgctac gagattattc
    38461 tcgccgagca agtgcgcgat aacttccgcc cggataccga ctacctgctg actatctcca
    38521 acgatgcgtg gtttggtaaa tctattggtc catggcaaca cttccagatg gcgcgaatgc
    38581 gtgcgctgga gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga
    38641 ttggcccgca gggtgagatt caggcgatga tcccgcagtt cgcccgcgag gtgttaacca
    38701 ctaacgtgac gccgaccacc ggactcacac catacgcacg taccggcaac tggccgctgt
    38761 gggtgctgac ggcactgttt ggttttgctg ctgtgttgat gagtctgcgt cagcgacgta
    38821 aataatccct tcttatcgtg ccggaataca tcccatattt cggcacgctc accggatgcg
    38881 gcatcagtgc attatccgga taacaatatt ctcctatcca actttggcac atctattgct
    38941 ttgttataca aggcaaagct tgaaccagca tcagtacaac ccggttgcac cacagcaatc
    39001 caccggtagc accaaaagag tgcattttaa gaattttgct tcagaatggt gcggtgcacc
    39061 tcgcaaaaac aaacaataaa acatcaatat ctttacattt agccaaacta aatgttacct
    39121 aacaggcaca acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg
    39181 gtatccatgc gttcttaacg cagaagataa aggtgttgga tatgcaatta cgtaaacctg
    39241 ccacagcaat cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc
    39301 cggcagcagg cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc
    39361 gtgaatcttc agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc
    39421 aggattactc caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc
    39481 aggtaaaact gattccgatt acctcacaaa atcgtattcc actgctgcaa aacggcactt
    39541 tcgattttga atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct
    39601 ctgacactat tttcgtggtc ggtacgcgcc tgttgaccaa aaagggcggc gatatcaaag
    39661 attttgccga cctgaaaggc aaagccgtag tcgtcacttc cggcactacc tctgaagttt
    39721 tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc
    39781 acggtgactc tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgacg
    39841 ctctgctggc cggtgaacgt gcgaaagcga agaaaccaga caactgggat atcgtcggca
    39901 agccgcagtc tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa
    39961 agctgatgga tgacactatc gctcaggtgc agacctccgg tgaagcggaa aaatggtttg
    40021 ataaatggtt caaaaatcca attccgccga aaaacctgaa catgaatttc gaactgtcag
    40081 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac
    40141 caggggcgga aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga
    40201 tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag
    40261 cagttaacgc tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg
    40321 ggtatttttt tacaacaagc cccgttcggc aataccacct atcttggttg ggtctggagt
    40381 ggttttcagg tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt
    40441 tcatttttcg gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg
    40501 tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg
    40561 atcccggagc tgctgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat
    40621 attcagtttt tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc
    40681 gaacaggtac gcgccgctat tcagtcgcta ccgcgtgggc agaagaatgc cgcgctggcg
    40741 atgggcctga cgctaccgca gacttatcga tacgtactgc tgcctaatgc ttatcgcgtt
    40801 atcgtcccgc cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg
    40861 accatcggtc tggtggatat ggcggcgcag gcgggtaaat tgctcgacta ctcggctcac
    40921 gcctgggagt cgtttaccgc catcacgctt gcgtatgtgt tgattaacgc ttttatcatg
    40981 ctggtgatga cgctggttga acgtaaagtt cgcctgcccg gcaacatggg gggcaaataa
    41041 tgtacgagtt tgactggagt tccattgttc cttccctgcc atatctgctc gacgggctgg
    41101 tgatcaccct gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggtaccatgc
    41161 tggcggtgat gcgcttgtcc agcttcgcgc ctgtttcgtg gtttgccaaa gcctacgtta
    41221 acgtattccg ctcgattcct ttagtcatgg ttttgctgtg gttttacttg attgtgccgg
    41281 gatttctgca aaatgtgctg ggattatcgc caaaaaatga tattcgttta atttcggcga
    41341 tggtggcgtt ttcgatgttt gaagcggcct actattcaga gattatccgt gccggtattc
    41401 aaagtatttc ccgtggtcag tcgagcgccg cgctggcgtt ggggatgact cactggcagt
    41461 cgatgaaact gattattctg ccgcaggctt tccgcgcgat ggtgccgctg ctgctcactc
    41521 agggcatcgt actgttccag gatacctcgc tggtgtatgt gttatctctg gccgatttct
    41581 tccgtaccgc ctcaaccatt ggtgagcgtg atggtacgca ggtcgagatg atcctgtttg
    41641 ccggatttgt ttatttcgtt attagcctta gcgcgtcgtt gttggtcagc tacttgaaaa
    41701 gaaggacagc ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt
    41761 gctgaccgac tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc
    41821 tggttccggc aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg
    41881 tgaaatcacc gtcgatggta tcgtggttaa cgacaagaaa accgatctgg caaagctgcg
    41941 ctcccgcgtc gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga
    42001 aaacctgacc ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa
    42061 agcattgaaa ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca
    42121 gctttccggc ggtcagcagc agcgcgtggc aatcgctcgc gcattgtgta tggctcctat
    42181 tgcgatgctg ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct
    42241 ggacgtgatg gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat
    42301 gggctttgcc cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga
    42361 agactcgccg aaagacgctt tcttcgatga tccgaaatcg gaccgcgcaa aagacttcct
    42421 cgcgaaaatc ctgcattaat cactctggcg cacataccca attgcgcgcc atctcacgct
    42481 ttatcgtttc gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac
    42541 acaacaagga gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgatga
    42601 cgctatcgca atagttctcg cactcgcctc accagagctt gatgtcaaag caattacgtc
    42661 ttccgccgga aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt
    42721 gcttaatcgc accgatattc cggtggcagg cggcgcggta aaaccgttaa tgcgtgagtt
    42781 gattatcgcg gacaatgtgc acggcgaaag cggtctcgac ggcccggcat taccggaacc
    42841 gacgttcgca ccgcaaaact gtacggcggt ggagctgatg gcgaaaacgc tgcgcgaaag
    42901 tgcggaacct gtcacgattg tttctaccgg accgcaaact aacgttgcct tgctgctcaa
    42961 tagccatccg gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg
    43021 gcttggtaac tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga
    43081 aattgtcttc cagtcaggga tcccggtggt gatggccggt ctggatgtta ctcataaagc
    43141 gcaaatccac gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat
    43201 tgttgccgaa ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt
    43261 cggcgcaccg ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac
    43321 cactgttgag cgctgggttg gcgtggaaac acaggggaaa tatacccagg gtatgacggt
    43381 tgttgattat tattatctga ctggcaataa accgaatgcc actgtaattg tcgatgttga
    43441 tcgtcagggc tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacactcgtc
    43501 attgattaag atcaccacgc gttacctggg tggtcttaat ctatttatct ctttttcttt
    43561 caagatataa atgcaatgga taatgcagaa ctcgccatag gtatcgatct cggcactacc
    43621 aatagtttaa ttgccgtctg gaaagacggt gccgcgcaat taattccaaa taagttcggt
    43681 gaatatttaa caccatccat aattagcatg gatgaaaata atcatatttt agtcggaaaa
    43741 ccggctgtat cacggcgtac ttcgcatccg gataaaacgg cagcgttatt taaacgtgca
    43801 atgggcagta ataccaactg gcggttaggc agcgacacat ttaacgcgcc agaactgtcc
    43861 tctttggtat tacgctcatt aaaagaagat gccgaagaat ttctgcaacg tccgattaaa
    43921 gatgtggtga tctccgttct ggcttatttc agcgatgaac aacgcaagca tacccgttta
    43981 gcagcggact tagccgggtt aaatgcggta cgcttaatta atgaacccac agcagctgcg
    44041 atggcgtatg gcctgcatac ccaacaaaat acccgttcgc tggtgtttga tctcggtggc
    44101 ggcacgtttg acgttacggt gcttgagtac gccacgccgg tgattgaagt tcacgcctcc
    44161 gctggcgaca acttcctcgg tggcgaagat tttacccata tgttggtcga tgacgtttta
    44221 aagcgcgcgg atgtcacaag gaccacgctt aacgagagtg aactggcagc cttgtacgcc
    44281 tgtgtggaag cggcaaaatg tagcaatcaa tcgccaatgc acattcgctg gtagtaccag
    44341 gatgaaacgc gggaatgcga attttacgag aacgaactgg aagatttgtg gttgccgctg
    44401 ctcaatcgct tgcgagtgcc gattgaacag gcgttgcgcg atgcgcgtct gaagccgagt
    44461 caaatcgaca gtctggtgct ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc
    44521 gccgtgcgtc tgtttggcaa attaccgtat caaagttacg atccgagcac cattgtcgcg
    44581 ctgggcgcag caatccaggc cgcctgccgc ttacgcagtg aagatattga agaagtgatc
    44641 ctcactgata tttgccctta ctcgttgggc gttgaggtta accgccaggg cgtttccggc
    44701 attttctcgc cgattattga acgaaacacc actgtgcccg tgtcgcgtgt agaaacttat
    44761 tcaaccatgc acccggaaca ggattcaatt acggttaacg tctatcaggg agaaaaccac
    44821 aaagttaaaa acaacattct ggtggaatcc ttcgatgtgc cgttgaagaa aaccggggct
    44881 tatcagtcga ttgatattcg ctttagttat gatatcaacg ggttgcttga agttgacgtg
    44941 cttctggaag acggcagcgt taagtccaga gtgattaacc acagcccggt aacattgagc
    45001 gcgcagcaga ttgaagagag tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat
    45061 atgctcatca atcgcacctt taaagccaaa ctggaagagt tgtgggcgcg ggcgctgggt
    45121 gacgagcgag aagagattgg ccgggtgatc accgattttg atgcggcgct gcagtcaaac
    45181 gatatggccc gcgtcgatga ggtcaggcgg cgggcgagcg tttatttagc cattgagacc
    45241 tcataatcac ggctgccgga tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca
    45301 catcgtcaac taaacaacat cgcgattttg ccgagagcat aacctataac cagcaccgtt
    45361 aaactgattt tgataatgct ggtaatgctc tgtggttttt caccctcatc atttttttct
    45421 tcatggtttt caggtgttgg ctcatcgaca tcttcatcct ccggctcgag ttttactttc
    45481 gtcaacagca tccgggtgat aatggcctct aatggatgag catctccgcc ttccagcctt
    45541 gtattaagac gggccagcat ctcttgtttc ggaaacgctt caagtactac ctgttccgct
    45601 acccgataca aatccagtaa cggtgaatct tcttctgtct cgctaccgac tctcgatttc
    45661 caccacgccc atgctttgcg tttgaagaat aacgactcaa taaacgattg acctaacggc
    45721 gataattttc cgctatgtaa aatttccgcc cataacagcc gcgtccgggc gctctcacca
    45781 ggaacatatt tcagcggttg accttgcgca tctaccatgc tccgcgcttc gataaccatc
    45841 actaataacg cgaaataatc cgggcaatga cgcttgctcc actgcaacaa caaattatca
    45901 gcttgtgtag atgggtaact ttcccagtac gcgccgagat cagcaagcag gctttccccc
    45961 tcgccgcagt acaaacgttg tgcacactgg taataccggg catcttcgct ttctggttct
    46021 tccgcttgcc actgtttggc tacaggaatg agttccacca tacctgtttg caccgagctg
    46081 taccagcgca acagtttgcg atgtaacttt gcatcatcag gaatgaacca cggtccttcc
    46141 atcgccaaaa acgcagtgac atagtcagga tggtaacggc agcaacgttc cagcgcgaag
    46201 taatattcaa cagtctggtt ctgtaacgcg acgggatatt ccagaaggct aaggaaatcg
    46261 aaacaatcac cagcctgaat ggcctcaaga aaagcctccc tttcttcctc atccattcct
    46321 tcgctgtcat taaacgaatg ccagttcaaa cgctctgcca acaaagaagc acaatttaat
    46381 gacagatatc gcgcttctat agcgattgca cacagcggcc agcgtaattg atcgacatcc
    46441 tccatgttcc aggtatttaa ttgctgaata aatttctgcc aggcggaggg ctgaaaacga
    46501 tcactttctg aatccagtaa tgtcctgaat gcacgtagta tttcatgttc agcggtggca
    46561 tctttttctt catcatctgc ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca
    46621 taggcctggc gtaatgcttt aaacccctgc ggatcggttt cgggatgaca caacggtaag
    46681 cgagcaagat aagcctggcg gataatgtct atttgcgtcg tgctttcaat ttcgaggatt
    46741 tgccaacatg ttttcatagg tcgctcacat ccttaagcgt taccgggcat tcaggctgga
    46801 cagttaatgg ctgccaggga agcgtggcat agaaacggtc gcttcccgct gccagctctt
    46861 cgaacatccg actcccggcg ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag
    46921 cgttgtcttc gcactgagaa gattgccagt agagacgccc tagtgaatag gcagaaaaat
    46981 agtgcatcca gctgtgatag taatggtgag ctcgtgcata gacgcgcgat tgcagccata
    47041 aactctcttc ttccgtcagc cagttgttga gcacaccggc ccgtgacaga aatcccattc
    47101 gaacataatc ccaggctttg attccccctt ctccacagca catagcggtt tgggcaacga
    47161 attgggtata aatcaaccct ctctccgagc caccagcaat taatgctttc cactcttcag
    47221 gggaagagcg ttgccatgtc agatataagc cagctaaatc attggcatga ccatcatcag
    47281 tcatctgata aatagtctga attaaatcat cacgattatt tattccccag gatgatgaga
    47341 gatcgaattc ttttatcccg gtatacattt ttggcgagtc atgcgcgggt ttgcaactct
    47401 gattaagtat ttccatgggc gcagaaagcg caaaaagaag atattgttct tccttattca
    47461 ttttttaata atcccttaag tgcattaact gactactttt ttattattga tgagtataaa
    47521 cttgacgctt tcaaaataaa aagaaaatcg aagcattcac acatgaataa aaaattaatg
    47581 tatatattcg caatttttat agttgcagca attacctgta ttagccaacc caagaaaacg
    47641 atgttgcgtg ataaagccat ggtgaattat gcctttgatt atttaagatc accaggcagt
    47701 ctaccgttaa ccacggcagc cacggagctt tccgcgattc atggtcactc aacatcgcaa
    47761 tatcgccttg gcgaatttta tcttcatggt agcgacggta aaccactgga ttatacacag
    47821 gcgagatact ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg
    47881 gggtggatct acctgaaagg cctgggagtc aaacccgata cccgtaaagc aattctctgg
    47941 tataaggaag cagctgaaca agggtatgct catgctcaat atactttggg tttgatctac
    48001 agaaatggca ctggtattaa tgttaaccat tatgaatctc aaaaatggtt aaaactggcc
    48061 gccaaacaac attacaaaaa tgcggaaaga ttacttgccg gacttcccgc acattaacgt
    48121 cgactgtcac ccgaagagat ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta
    48181 gaaaataatt ttgatgacgc ccagacagcc cagcccgcca tcatcaacgg gcattggctg
    48241 cggttgcggc tccggctcga cggtttcctg accggtaaaa ttcgccgcct gaattaataa
    48301 tcgacgaata attaatgctt ccagtggaca acgatcttct gcatcaaggc ggctcacaat
    48361 cggcattaat gattcttctg tcggttggct ggcctgcact aattgtttgg caactaaata
    48421 taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct tacgtccttt
    48481 gtgcaaaacc atctcgacca gtgaacgccc cagcgcagag agtttttcat cgctgagtat
    48541 ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg acataaagta gcggttttcc
    48601 ctgatcgtta accagatcac gcgcttcaat catcatcacc actaatgggt aataatagac
    48661 ccgatgctga cggcaccatt gcaacattaa agcatccgcc tgggtggagg gatactcgcg
    48721 ccagtagtaa caaagttctg ccagcaggct ttcgccttct ccacaataga cgcgttgtgc
    48781 gtattcataa taacgcgcat cttcattatc aggttcagct tcccgccacg accgggcgta
    48841 atccaggagt tcggggattc ccaggcggct tgcgctatac cagcggagta aatttctgtg
    48901 cagttttgca tcatcgggga taatcactgc gcgatgttgc gctaaataca atgtcagcca
    48961 ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg tgtcattttg
    49021 cacggcaact ggcagatgca gaatggtctg gaagttaaaa acattcccct tcgcaatggc
    49081 atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct cctgcaacca
    49141 ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag tgttcatggc
    49201 gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat aatttaattg
    49261 ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg caagtaacgc
    49321 gcgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt gccaaacaga
    49381 tttagcaggc gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa
    49441 accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag cgcggcggat
    49501 aatatcgacg tcagtcgttt cctctatatc gaggatcttc cagcaatttt tcataagtcg
    49561 ctcatatcct taagcgtttc cgggcatgtg gggtagtcag ccagtggttg ccaggacaag
    49621 gtagggtaat acgcatcatc actggcaaat aattgataaa acatatcatt gccggaatcg
    49681 tcgtattctt tacgcgctaa gaattcgcgt aataaaggca gatgatcatc ttcgggagac
    49741 tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt actataataa
    49801 cgtagagccc gtagatgaat acgcgactgg atccatagac tttcttcttc actcaaccag
    49861 ttattaagta cgcccattcg acttaaaaaa cccatccgga cataatccca tgctttaatt
    49921 cctccctcac cacagcattc ggctgtgctg gcgacaaatt gcgcataagc ctgaccttgc
    49981 tcatttaatt cagcaagata gtcacgccat tcacatggag aataacgaaa ccagcggtga
    50041 taaaaccctg ccaaccgagc ggcatgaccg ttctcgatca ttcggtgaat tatgcgcagc
    50101 aggtcatcac ggtcatcaat tccccatgag gaagaaaggt caaaaaagca tgtacctaaa
    50161 tacattttgg gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc catcggagtg
    50221 gaaagtgcaa acaatatttt ttgcgacccc atgtccatat aatttatcct taaacattca
    50281 tttaatgaat gtaaactaca tttcgaggct cagttattta tacttttctg acgcaacata
    50341 atacgcataa aataaggctt acgcaaaaat ggaacacata aaaatataat atattttatc
    50401 tacaaccata aggaaatgat aatgattttt acgtcaagtt gctgcgataa tttatcaata
    50461 gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt
    50521 tattataatc gcgatagcgc aattgactct ccggacgacg aaaaggcctt ttactggctg
    50581 aagctggccg ctgagcaagg tcattgtgaa gcacagtatt ccttagggca aaagtatacc
    50641 gaggataaaa gccgtcataa agataatgag caagccatct tctggctgaa aaaagctgcc
    50701 ctacaaggcc atactttcgc ttccaacgcc cttggctgga tactggatcg tggagaagac
    50761 cccaactata aagaagcggt tacctggtat cagatagccg cggagagcgg aatgtcttat
    50821 gcgcaaaata atcttggatg gatgtacaga aatggcaacg gagtcgcaca agactatacg
    50881 ctggcatttt tctggtacaa acaagctgca ttacaaggcc atagttacgc acaagacaat
    50941 ctggccgatc tttataaaga cggagaaggc gttgctcaaa acaagacact cgccgcattc
    51001 tggtatttga aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat
    51061 tataacgctg gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag
    51121 gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga
    51181 caaggcgttg agaaagatta tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc
    51241 aatgacgcca ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct
    51301 gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat
    51361 gtcagtcagg aaattcgtga gactgaagct ttactgtaga aataaaaaca agggccgggg
    51421 aatttcattc tgcccatatt ctctttattg ctgtgcgaaa tcagactatt acggctcaaa
    51481 cgggcgtctc tggaactggt cataaccaca tttcgggcat agcgtcagca cttccggtgt
    51541 gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc
    51601 tccgctgtga taaaccccat gatgattgag gtcctggaaa atttcgcgcc attcaagctg
    51661 cgttttatcg gtgatgtctg ccagctcctg ccacaagctt tctttaatca cccgcataaa
    51721 gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc
    51781 acgtctgaca gctcgcgtca gctcatcgat ctcggttcgc gttaactccc ctgtttttat
    51841 tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg
    51901 ttcgttcagt gacgcgacca gttcacggta atattgagca accttgttca tcgtttcatc
    51961 tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg
    52021 cccacaaact ctgtaaatta atgtacgaat aatccgggca tcatttcaac gtcgagtgcg
    52081 cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc
    52141 aacgctacat ttgtagccgt attgaaaaca ggaccactgg ctgccatgca agagcaatac
    52201 cgcccggaag agatagaatc caaagtacag cttcattggg atgagaagcg cacatttgaa
    52261 gtaaccgaag acgagagcaa agagaagtat tactgcctgt ctatgcttcc ctatccttct
    52321 ggtcgactac acatgggcca cgtacgtaac tacaccatcg gtgacgtgat cgcccgttac
    52381 cagcgcatgc tgggcaaaaa cgttctgcag ccgatcggct gggacgcgtt tggtctgcct
    52441 gcggaaggcg cggcggtgaa aaacaacacc gctccggcac cgtggacgta cgacaacatc
    52501 gcgtatatga aaaaccagct caaaatgctg ggctttggtt atgactggag ccgcgagctg
    52561 gcaacctgta cgccggaata ctaccgttgg gaacagaaat tcttcaccga gctgtataaa
    52621 aaaggcctgg tatataaaaa gacttctgcg gtcaactggt gcccgaacga ccagaccgta
    52681 ctggcgaacg aacaagttat cgacggctgc tgctggcgct gcgataccaa agttgaacgt
    52741 aaagagatcc cgcagtggtt tatcaaaatc actgcttacg ctgacgagct gctcaacgat
    52801 ctggataaac tggatcactg gccagacacc gttaaaacca tgcagcgtaa ctggatcggt
    52861 cgttccgaag gcgtggagat caccttcaac gttaacgact atgacaacac gctgaccgtt
    52921 tacactaccc gcccggacac ctttatgggt tgtacctacc tggcggtagc tgcgggtcat
    52981 ccgctggcgc agaaagcggc ggaaaataat cctgaactgg cggcctttat tgacgaatgc
    53041 cgtaacacca aagttgccga agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact
    53101 ggctttaaag ctgttcaccc attaacgggc gaagaaattc ccgtttgggc agcaaacttc
    53161 gtattgatgg agtacggcac gggcgcagtt atggcggttc cgggtcacga ccagcgcgac
    53221 tacgagtttg cctctaaata cggcctgaac atcaaaccgg ttatcctggc ggctgacggc
    53281 tctgagccag atctttctca gcaagccctg actgaaaaag gcgtgctgtt caactctggc
    53341 gagttcaacg gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa actgactgcg
    53401 atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt ttctcgccag
    53461 cgttactggg gcgcgccgat tccgatggtg acgctggaag acggtaccgt aatgccaacc
    53521 ccggacgacc agctgccggt gatcctgccg gaagatgtgg tgatggacgg cattaccagc
    53581 ccgattaaag cagatccgga gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt
    53641 gaaaccgaca ctttcgacac ctttatggag tcctcctggt actatgcgcg ctacacttgc
    53701 ccagagtaca aagaaggtat gctggattcc gaagcggcta actactggct gccggtggat
    53761 atctacattg gtggtatcga acacgccatt atgcacctgc tctacttccg cttcttccac
    53821 aaactgatgc gtgatgcagg catggtgaac tctgacgaac cagcgaaaca gttgctgtgt
    53881 cagggtatgg tgctggcaga tgccttctac tatgttggcg aaaacggcga acgtaactgg
    53941 gtttccccgg ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa
    54001 gatgcggcag gccatgaact ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac
    54061 aacggtatcg acccgcaggt gatggttgaa cgttacggcg cggacaccgt tcgtctgttt
    54121 atgatgtttg cttctccggc tgatatgact ctcgaatggc aggaatccgg tgtggaaggg
    54181 gctaaccgct tcctgaaacg tgtctggaaa ctggtgtacg agcacacagc aaaaggtgat
    54241 gttgcggcac tgaacgttga tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg
    54301 cataaaacta tcgctaaagt gaccgatgat atcggccgtc gtcagacctt caacaccgca
    54361 attgcggcga ttatggagct gatgaacaaa ctggcgaaag caccgaccga tggcgagcag
    54421 gatcgcgctc tgatgcagga agcactgcag gccgttgtcc gtatgctcaa cccgttcacc
    54481 ccgcacatct gcttcacgct gtggcaggaa ctcaaaggcg aaggcgatat cgacaacgcg
    54541 ccgtggccgg ttgctgacga aaaagcgatg gtggaagact ccacgctggt cgtggtgcag
    54601 gttaacggta aagtccgtgc caaaatcacc gtcccggtgg acgcaacgga agaacaggtt
    54661 cgcgaacgtg ctggccagga acatctggta gcaaaatatc ttgatggcgt tactgtacgt
    54721 aaagtgattt acgtaccagg taaactcctc aatctggtcg ttggctaagc gcgggaggaa
    54781 gcgtgcgata tctggcaaca ctgttgttat ctctggcggt gttaatcacc gccgggtgtg
    54841 gctggcatct gcgtgacacc acgcaggttc cttccactat gaaggtcatg atcctggact
    54901 caggcgatcc gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg
    54961 tcgagttgct tgataaagaa accacgcgta aggacgttcc atccctgcgt ttgggtaaag
    55021 tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg tcaaacagca gagtatcaga
    55081 tgatcatgac ggtgaatgcg accgtgttga tccccggccg tgatatctac ccgattagcg
    55141 ccaaagtctt ccgttcattc ttcgataacc cgcaaatggc gttagcgaaa gataacgaac
    55201 aagacatgat cgtaaaagag atgtacgacc gtgctgccga acagctgatt cgtaagctgc
    55261 caagcatccg tgctgcggat attcgttccg acgaagaaca gacgtcgacc acaacggata
    55321 ctccggcaac gcctgcacgc gtctccacca tgctgggtaa ctgatgattc ggttgtaccc
    55381 ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa
    55441 cgatcctctg ttattgcagg aaagccagga cgctgttcgc caggtagctg cggcacaagg
    55501 attcgaagaa caccacactt tttccattga tcccaacact gactggaatg cgatcttttc
    55561 gttatgccag gctatgagtc tgtttgccag tcgacaaacg ctattgctgt tgttaccaga
    55621 aaacggaccg aatgcggcga tcaatgaaca acttctcaca ctcaccggac ttctgcatga
    55681 cgacctgcta ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg
    55741 gtttactgct cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg agcaggctca
    55801 gcttccccgc tgggttgctg tgcgcgcaaa acagctcaac ttagaactgg atgacgcggc
    55861 gaatcaggtg ctctgctact gttatgaagg taacctgctg gcgctggctc aggcactgga
    55921 gcgtttatcg ctgctctggc cagacggcaa actgacatta ccgcgcgttg aacaggcggt
    55981 gaatgatgcc gcgcatttca ccccttttca ttgggttgat gctttgttga tgggaaaaag
    56041 taagcgcgcg ttgcatattc ttcagcaact gcgtctggaa ggcagcgagc cggttatttt
    56101 gttgcgcaca ttacaacgtg aactgttgtt actggttaac ctgaaacgcc agtctgccca
    56161 tacgccactg cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc ggggcatgat
    56221 gggcgaggcg ttaaatcgct taagtcagcc gcagttacgt caggccgtgc aactcctgac
    56281 acgaacggaa ctcaccctca aacaagatta cggtcagtca gtgtgggcag agctggaagg
    56341 gttatctctt ctgttgtgcc ataaacccct ggcggacgta tttatcgacg gttgatatga
    56401 aatctttaca ggctctgttt ggcggcacct ttgatccggt gcactatggt catctaaaac
    56461 ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt cacaatcatc cctaataatg
    56521 ttcctccgca tcgtccccag ccggaagcga acagcgtgca gcgtaaacac atgcttgaac
    56581 tggcgattgc cgacaagcca ttatttactc ttgatgaacg cgagctaaag cgcaatgccc
    56641 cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc
    56701 tggcgtttat tattggtcag gattcattgc tgacctttcc gacctggtac gaatacgaaa
    56761 cgatactcga caatgcacat ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg
    56821 cgcaaccgca ataccagcaa tggctggaag atcatttgac acataacccg gaagatcttc
    56881 accttcagcc tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac atctcggcga
    56941 ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga ggatttattg ccggaaccgg
    57001 tattgactta cattaaccaa caaggcttgt atcgctgata cctgtcgtta cggagggcat
    57061 gatgcgactg tggttaattc gtcatggcga aacacaagcg aatgtcgatg gtctgtacag
    57121 cggtcatgcg cccacacccc tgaccgcacg cggtattgag caagcgcaaa atctgcatac
    57181 actgctagat gatgtttcct ttgacttggt tttatgcagt gaactggaac gggcacagca
    57241 taccgcgcga ctggttctca gtgaccgcca gctccccgtt catatcatcc ctgaactcaa
    57301 cgaaatgttt tttggcgact gggagatgcg acatcatcgc gacctcatgc aagaagatgc
    57361 cgaaaactat agcgcgtggt gcaatgactg gcagcatgct atccccacga acggtgaagg
    57421 atttcaggca ttttcgcaac gtgtggaacg ctttatcgca aggcttagtg aatatcagca
    57481 ctatcagaat attttaatcg tcagccatca gggcgtactg agtctgttaa ttgctcgttt
    57541 aattggcatg cctgccgagt ctatgtggca ttttcgcgtt gaccaggggt gttggagcgc
    57601 cattgatatc aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt
    57661 cgagaacgca tgacttttct gtttttttac gggtaagccg caacgaccat tgacagcccc
    57721 gggcaggctg atattctccg cagccagact ttttccgcca gacacgactt tgtagaaatt
    57781 gttttacaaa aatggcgatg caatctgcgg cgcggggtgg gatgatagcc cactttcgaa
    57841 agccgattcg gcgacaattg tcccgaaatc gcctctggtt caggtatact gacagaccat
    57901 ttttatctat ttgattcacc cagggggaaa acttgcaggg taaagcactc caggattttg
    57961 ttatcgacaa aattgatgac ctcaaaggtc aggacatcat cgccttagac gttcaaggca
    58021 aatccagcat caccgactgc atgatcatct gtacgggtac gtccagccgt catgttatgt
    58081 ccattgctga ccacgttgtg caggagtctc gcgcagcggg cctgttaccg ctcggcgtag
    58141 aaggtgaaaa cagcgccgac tggattgtcg tggatttggg cgatgtgatt gtccatgtca
    58201 tgcaggaaga gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc
    58261 tgcaacttgt cgccgtggga acgaaaatgc cggactgggt acaaaccggt tttaccgagt
    58321 acctgcgtcg ttttccgaaa gatatgccct tcgagctgat tgaaattccg gccggcaaac
    58381 gcggcaagaa tgcggacatc aagcgcatac tcgacaaaga gggtgagcag atgttggcgg
    58441 ccgcaggcaa aaaccgcatt gtcaccctcg atattccagg caagccctgg gatacgccgc
    58501 agttagccgc tgagctggaa cgctggaagc tggatggtcg cgacgtaagt ctactgattg
    58561 gcgggcctga agggttgtcg cctgcctgta aagcggcggc tgagcagagc tggtcgctgt
    58621 cggcgcttac cctcccccat ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg
    58681 cgtggagcat caccaccaac catccttatc accgtgagtg ataagggagc tttgagtaga
    58741 aaacgcagcg gatgaaacta cagaactctt ttcgcgacta tacggcagag tccgcgctgt
    58801 ttgtgcgccg ggcgctggtc gcctttttgg ggattttgct gctgaccggc gtgcttatcg
    58861 ccaacctgta taatctgcaa attgttcgct ttaccgacta ccagacccgc tctaatgaaa
    58921 accgcattaa gctggtgcct atcgcgccca gccgcggcat tatctacgac cgtaacggta
    58981 tccctctggc cctcaaccgc actatctacc agatagaaat gatgccggag aaagtcgata
    59041 acgtgcagca aacgctggac gctttgcgca gcgtggtaga tctgaccgat gacgatattg
    59101 ctgcattccg aaaagagcgc gcacgttcac accgtttcac ctctattccg gtgaaaacta
    59161 acctaaccga agtacaagta gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg
    59221 aagttaaagg ctataaacgt cgttactatc cttacggttc ggcgttgacc cacgtcatcg
    59281 gctatgtgtc gaaaatcaac gataaagacg tcgaacgcct gaataatgac ggcaaactgg
    59341 ccaactatgc ggcaacgcat gatattggta agctgggcat tgagcgttac tatgaagacg
    59401 tgctgcacgg tcagaccggt tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc
    59461 gccagttaaa agaagtacca ccgcaagccg gacacgatat ttacctgacg ctggatctca
    59521 aactccagca atatattgaa acgctgctgg cgggtagccg cgcagctgtg gttgttaccg
    59581 aaccgcgtac aggtggggtg ctggcgctgg tttccacgcc tagttatgac ccaaacctgt
    59641 ttgttgacgg tatctccagc aaagattatt ccgccttgct gaacgacccg aatacaccgc
    59701 tggtgaaccg cgccacacag ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg
    59761 cggtttcggc attaagcgcc ggggtgatca cgcgcaatac gacgctgttt gacccaggct
    59821 ggtggcaact accagggtcg gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc
    59881 gtctgaatgt cacaagatcg ctggaagaat ctgcggatac cttcttctat caggtggcct
    59941 acgatatggg gatcgatcgc ctctccgaat ggatgggtaa attcggttat ggtcattaca
    60001 ccggtatcga cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga
    60061 aacgctttaa aaaaccgtgg tatcagggtg acaccattcc ggttggtatc ggccagggtt
    60121 actggacagc gaccccaatc cagatgagta aggcactgat gatcctgatt aatgacggta
    60181 tcgtgaaggt tccgcatttg ctgatgagca ccgccgaaga cggcaaacag gtgccatggg
    60241 tacaaccgca tgaaccgccc gtcggcgata ttcattccgg ttactgggag ctggcgaaag
    60301 acggtatgta cggtgttgct aaccgcccta atggtacggc gaataaatac tttgccagcg
    60361 caccgtacaa aattgcggcg aaatctggta ccgctcaggt cttcggtctg aaagcgaacg
    60421 aaacctataa tgcgcacaaa attgccgagc gtttacgtga ccacaaactg atgaccgcct
    60481 ttgcgccata caacaatccg caagtggctg tcgccatgat tctggagaac ggcggcgcgg
    60541 gtccggcggt tggtacgctg atgcgccaga tcctcgacca cattatgctg ggtgataaca
    60601 acaccgatct gcctgcggaa aatccagcgg ttgccgcagc ggaggaccat taatcatgac
    60661 ggataatccg aataaaaaaa cattctggga taaagtccat ctcgatccca caatgctgct
    60721 gattttactg gcattgctgg tttacagcgc cctggttatc tggagcgcca gcggtcagga
    60781 tattggcatg atggagcgta aaatcggcca aatcgcgatg ggtctggtca tcatggtggt
    60841 gatggcgcaa attcctccac gcgtttatga aggctgggcc ccctatctct atatcatctg
    60901 tattattttg ctggtggcgg tagatgcttt cggagccatc tctaaaggcg ctcaacgctg
    60961 gctggacctc ggtattgttc gttttcagcc gtcggaaatt gccaaaatag ccgtaccact
    61021 gatggttgcg cgctttatca accgcgacgt ttgcccgcca tcgttgaaga atactggcat
    61081 cgcgctggtg ctgatattta tgcccacgct gctggtggct gcacagcctg acctgggaac
    61141 atcaatcctc gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc ttagctggcg
    61201 tctgattggc gtcgcagtag tgctggtagc ggcgttcatt ccgattctgt ggttcttcct
    61261 gatgcatgat taccagcgcc agcgcgtaat gatgctcctg gacccggaat cagacccact
    61321 cggcgcgggc tatcacatta ttcagtctaa aattgctatt ggctccggcg gattacgcgg
    61381 caaaggctgg ctgcacggca ctcagtcaca gcttgaattt ctccccgaac gccatactga
    61441 ctttatcttc gcggtactgg cggaagagct gggattagtg ggcattctga ttctgctcgc
    61501 tctctacatt ctgctgatca tgcgcgggct gtggatagcc gccagagcgc aaaccacctt
    61561 tggtcgcgtc atggctggtg gcttaatgct gatattattc gtttatgtct tcgtaaatat
    61621 tggtatggta agcggtattc tgccggttgt aggggttccg ctcccactgg tcagttatgg
    61681 aggatcggcg ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa tccacaccca
    61741 caggaaaatg ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga
    61801 tctgcatcgc ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag caacagacgg
    61861 taagtgtacc gcagcctgcg gtatgtaacg gccccatagt tgaaattagc ggggcggacc
    61921 cgcgtttcga accactgaac gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct
    61981 acaaaatcgt gcaggatccg tctcgattta gccaggcggg actggcggca atctatgatg
    62041 ccgaaccagg cagtaacctg acggcctctg gcgaagcttt cgatccgaca aagctgacgg
    62101 cggcccatcc aacgcttccg atccccagct acgccagaat cactaacctg gctaacgggc
    62161 gaatgatcgt ggtgcgcatt aatgatcgcg gcccttacgg caacgaccgc gttatttcgc
    62221 tttctcgcgc ggcagctgac cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc
    62281 cgattattgt cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt accacagtcg
    62341 ccaaacagac ttacgccctg cctgcacctc ccgatttaag cggtggcgcg ggaacaagtt
    62401 cagtgtctgg cccgcagggt gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag
    62461 atccgaccgg cgcgccggta accagtagcg gtttcctcgg cgctccaacg accttagcgc
    62521 ctggtgtact ggaaggcagc gaaccgacgc ctgcgccaca gcccgttgtt acagcttcgt
    62581 cgacaacgcc tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg caaagcgcca
    62641 gcggcaactt tatggtgcaa gtcggggccg taagcgatca ggctcgtgcg caacagtacc
    62701 aacagcaact gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct
    62761 ggcggatcca gcttggccca ttcgccagca aagccgaagc cagtaccttg cagcaacgtt
    62821 tgcaaaccga agcccaatta cagtcattta ttactaccgc gcagtagcgt aaagcaggca
    62881 tctgatgtgt caatttctgt aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg
    62941 ccggtagtgc atttgctata gtagggcact tttttaattc catcacggat gtcgtagttc
    63001 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    63061 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa actatgatcc
    63121 cgggtgtacc gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag
    63181 tgctcgccga acagaacgca gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga
    63241 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    63301 ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    63361 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat
    63421 cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    63481 ttggcttgat gaacagctac gttaacgcac tgggcctgaa aaatacccac ttccagacgg
    63541 tacatggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc
    63601 aggcattgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    63661 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    63721 acggcatcaa aaccggacac actgacaaag caggttacaa tcttgttgct tctgcgactg
    63781 aaggccagat gcgcttgatt tctgcggtga tgggtggacg tacttttaaa ggccgtgaag
    63841 ccgaaagtaa aaaactgcta acctggggct tccgtttctt cgaaaccgtt aacccactga
    63901 aagtaggtaa agagttcgcc tctgaaccgg tttggtttag tgattctgat cgcgcttcgt
    63961 taggggttga taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag
    64021 ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    64081 gaactatcaa cttccagctt gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc
    64141 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    64201 atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa
    64261 gcatcagtaa aaaaactccc gccttctggc gggagttgct atttaattac gttacgccgg
    64321 agctgacatg aaaaccaaac ttaacgaact gcttgaattc cctactcctt ttacttacaa
    64381 agttatgggg caggcgttac ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca
    64441 tgcgccaggt gactacaccc caacggtaaa accaagcagc aaaggcaact accactcggt
    64501 atctatcact atcaacgcca ctcatatcga gcaggttgaa acactgtatg aagaactggg
    64561 caaaatcgat attgtccgca tggttctgta actcgcttct ccgttacccg gctcctggtc
    64621 gggtaactcc cctctcctcg ctgtgatata cttttccccc acttttactc attctccacg
    64681 gagatgccgt tttgtatcag gataaaattc ttgtccgcca gctcggtctt cagccttacg
    64741 agccaatctc ccgggctatg catgaattca ccgatacccg cgataatagt acccttgatg
    64801 aaatctggct ggtcgagcac tatccggtat tcacccaagg tcaggcagga aaagcggagc
    64861 acattttaat gccgggtgat attccggtga tccagagcga tcgcggtggg caggtgactt
    64921 atcacgggcc gggacaacag gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg
    64981 gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt gaataccctg gctgaactgg
    65041 gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt
    65101 gctcactggg tttacgaatt cgacgcggtt gttcattcca cggtctggca ttaaacgtca
    65161 atatggatct ttcaccattt ttacgtatta atccttgtgg gtatgccgga atggaaatgg
    65221 ctaaaatatc acaatggaaa cccgaagcga cgactaataa tattgctcca cgtttactgg
    65281 aaaatatttt agcgctacta aacaatccgg acttcgaata tattaccgct taattccata
    65341 catcaatggc ccaatttaca ctgggtcatt actcataata cctgctaatg ctttacatct
    65401 ccttcgcgtt tgtatatttt ctttatcact tgcagaaatg cggtgccacg ccttgctccg
    65461 taactcaccc cattgattaa cctattgaaa tagttgatta tggtgagcaa aagcgttccg
    65521 ttccaattag acataagatt attgtaattt tttaaatatt atttttccat gaataaaatt
    65581 ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag
    65641 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaacctt ctgactattt
    65701 ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc
    65761 cctcggcaat cagtcagtct attcagaaac tgcgcgttat attccctgac ccattgttta
    65821 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca
    65881 gtcagggcct tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    65941 gaacgataac tattgcgacc actccctcgg tcggagccct ggtccttcct gtcatctatc
    66001 gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg
    66061 aaaaccaact cagtcagttt caaaccgatc tcatcatcga taacatgttt tgcaccaatc
    66121 gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcaagggaa
    66181 atccactact ctctttagaa gatgacagag agactatcga caacgctgcg catgtacttc
    66241 tgttaccgga agggcaaaat ttcagcggtc tgcggcagag agttcaagag atgtttccgg
    66301 accggcaaat caatttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca
    66361 gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagtcgt tgctggccgc
    66421 tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca
    66481 acaaattcat cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat
    66541 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
    66601 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
    66661 tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
    66721 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
    66781 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
    66841 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
    66901 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
    66961 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
    67021 ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta
    67081 gctcctgatg ccaatgaacc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt
    67141 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt
    67201 gcggattgta ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
    67261 ccggatttcc gcggtcgaat ggatcgtgct ctggatattc tgactgcaac gcccccagat
    67321 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca
    67381 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
    67441 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
    67501 cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc
    67561 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
    67621 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac
    67681 cacgccgatt tgcaggcgaa aggaatggaa gttaagtaat cctgtaataa ttacttacat
    67741 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
    67801 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
    67861 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
    67921 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
    67981 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
    68041 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
    68101 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
    68161 ttgcggcggc gcaaagcgac ggttgtttaa gatgggcagt tgcgcgcgca cctgacgcac
    68221 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
    68281 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
    68341 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accagtgatg
    68401 ctctttgagc ggcccacgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
    68461 agcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
    68521 catcccttcc acctccagta acggagctat ttcattacca gcatcaacat ggcgtgattc
    68581 ctgaatggca aatgcatcat agagatgcag tttggcataa cgggcgacga tgtttcctgc
    68641 ctgaagtgcc accagcatat tccatgcgcg ccccggcgtt gaaggaagat gaatcgtcag
    68701 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
    68761 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc
    68821 ttccggcagg acaaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
    68881 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcag caaccagcat
    68941 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
    69001 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    69061 ttggcggtgg tacgggaagc gtagcgagat ggctgttaag tatgcgattt aacccgctgc
    69121 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
    69181 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
    69241 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
    69301 tgttacaaga gggccgcttt ggctgggcat tactgaacgt tttcgtcaac cttctggggt
    69361 cttttgccat gaccgcactg gcattctggc tgttttcggc ctcaaccgta cactaaagac
    69421 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
    69481 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
    69541 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
    69601 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
    69661 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
    69721 cggttatcag tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat
    69781 aaaagtcgcg ttatttttga tgttgcttat tttagttatt gattttaaag aagttactaa
    69841 aacttcattt gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt
    69901 aggtacccgg aatgtaggtc atctgaaaag tcactgggcc ataacccacg gaggccaatg
    69961 gcagtagaac cgggagaggg atgtaattcc agttatcgcg tgccgttacg ccagcggtga
    70021 atcccagacc taaatgaaaa ttttcatccg ccagcggtcg ccaggtactt tcccatccgt
    70081 atccggcaat cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat
    70141 gccagtttcc tttttcatcc caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt
    70201 tatagcgatc ggttttttct ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa
    70261 tatataaatc ataatgttca ggctgttgcc aggtttgtgc aatattttct ctaaacgttg
    70321 tcatcctctc atctgcgtta gcaaaaactt taccaacgct gattaactga ataaaaacaa
    70381 aggaaaagat agcgacatat ttactcacgt tcatttgtga ccataaaaca tttatcaaaa
    70441 atctactact agcatagcaa agctacaatt aacataacct taatagaccc aacataaaga
    70501 ataatctgaa tagctgctct atttatttcc gcttgttcaa gttaaatatt tccgcgcatc
    70561 gattaaagat gatgcgtcgt tttctgcgat gggaatagtc aaaaaagaaa aaccaagtct
    70621 tttttgatga caaaatgcaa tcaaggaaaa attattttat ttttttaacg agatacaaca
    70681 atcatcttaa cgaagtatat aatatatatc gtcaacccat gctcatatta gattattttt
    70741 tgagcgcaca gccgcagcta acaccattgc aattaacaaa tttgcatcaa tccaccatca
    70801 atttacacac attattatgt gataattgcc aaccgctaaa tatgcgtttt gttatctatg
    70861 tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa tcagcattca
    70921 tcgccaattt attgggcata ttttttcctt aagctttagg aagtttttat tatttacttt
    70981 gggcctggag acaggaaaaa ttatgctgac attcattgaa ctccttattg gggttgtggt
    71041 tattgtgggt gtggctcgct acatcattaa agggtattcc gccactggtg tgttatttgt
    71101 cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt taccgtccag
    71161 ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa tattactaat
    71221 gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggctttg ccgcttacat
    71281 gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat tgcagtatat
    71341 taactcccct tacctgctga tgattgccgc ctattttgtt gcttgtctga tgtcactggc
    71401 cgtctcttcc gcaaccggtc tgggggtttt gctgatggca accctgtttc cggtgatggt
    71461 aaacgttggt atcagtcgtg gcgcagcagc tgccatttgt gcctccccgg cggcgattat
    71521 tctcgcaccg acttcagggg atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat
    71581 tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg gcatggcgat
    71641 cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc atgaaatgtt
    71701 agatgtcagt gaaatcacca ccactgcccc cgcgttttat gccattttgc cgttcacgcc
    71761 gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca tcatcactat
    71821 tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct ttaataccca
    71881 gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt ttgctaacgt
    71941 ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca tcggctttat
    72001 tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc tgatgctggt
    72061 attggtgatt ctgaccatgc tggcggcagt cacgaccggt tcaggcaatg cgccgtttta
    72121 tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc cggcgtattt
    72181 gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg tttctggcgt
    72241 agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc
    72301 ggtgccggtg cttgttgggc tagtgattgt tatcgttgct acagagctga tggtgccagg
    72361 aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt tcctccggct
    72421 ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc gtgaacaatt
    72481 tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc agtggtgcgg
    72541 ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg ctctttaaac
    72601 agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggcagttc gtctttgggt
    72661 tcaccgcgag cataggcatt aaacatctca tcaatttgct tctggctggc gctatcaata
    72721 ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag tgtattgatt
    72781 cacttgaagt acgaaaaaac cgggagaaca ttggattatt cgggatctga tgggattaga
    72841 tttggtgggg cttgcaagcc tgtagtacaa attttaggcg ttaaccaatg aaacgcgaaa
    72901 gatagtaaaa aattgctttt gtttcattga aaaaacgaaa aacaaaaaca ctataaatca
    72961 tttcaatcat agcttcaaaa aacgttcaaa accgataaca accaggctgt caccaaatga
    73021 ctcatatcac aaatcagctc atgccgttta ggtatgttac atgtgtgatt atgtgaggtg
    73081 aagtatgttt tagctggctc atggttgtta tacggctttt ttcgcatctt gtggttccaa
    73141 caaaggagct acaggctctt cctgtttatg aacaccatac attgacaaaa tctcttggcg
    73201 agtttctgga ggtaatttat gcagcgccaa agctgtttgt agtgtttggc gatcaatacc
    73261 cagcgttgcc aatagggtgt caacggagat ggaggggcgt tcttgcatta atccggaaag
    73321 cccatattta gccatgttta gctctatctc tcggacttca ttttctccta gtttattgat
    73381 taacagggag aacattttca atgtatcagc attcatttct tcgatagcaa ttttttgcat
    73441 agccggtatt gccgggatat attcaacccc aggcccgcga cgcccttctt gtgaacgctt
    73501 cggccatcct tctttctttg ctcgtttatg caccccctgt attgtcccag gcattccatc
    73561 cagttcagta atttgtttgg gtgtcatcca ctggtttatc tcatttgcgc ttttcataaa
    73621 ctaagtttac cagatttatc tatataaaaa tcaatggatt acaaaataaa ccacattaac
    73681 tgatataaac cggtttggtt tatttggtaa tatataaaac agataatcaa gacgaattat
    73741 ttgttttggt aaccctttat catacgcaat atggttggtg tgcgaaagat gagtaacgat
    73801 tggcatcggg ccgacgtgat tgcggccctc aaaaaaacag gaacccctct ggcaaaattg
    73861 tccagacgtt caggttattc aaccgggacg cttgcaaatg cattagtcag gccatggcct
    73921 aaaggtgaac gcattattgc tgacgcctta aacatgaaac ctgaggaaat ttggcctagt
    73981 agatatctaa gggatcacaa tgagagagtg gatatcagca attgaatgca tgggtttatc
    74041 tggtttacct ggttcattaa tgggggttca caaaagagct cagagagaag gatggctcaa
    74101 gagaagaaga gaaggaatta gaggggttgc ttacgaatac catatatctt ctcttcctaa
    74161 cattgctcga gctgaagttt tgctccaaca aggaaaattt aaaaccagtc agggatgttt
    74221 tgaaatcgcc cgccccacgc tggaagctca tgattatgat cgtgaggcac tatggagcaa
    74281 atgggataaa gccagcgatt cccagcgcag cctggctgaa aaatggttgc cctcaattca
    74341 ggccacagac gaaatgctga accaggggat ttcaacgaaa acggcttttg cgaccgttgc
    74401 agggcattac caggtcagcg catccacttt gcgggacaag tattaccagg tacagaagtt
    74461 tgcgaagcct gactgggcgg ctgcacttgt tgatggacgt ggagcatccc gtcgcaatgt
    74521 tcacaaaagt gaatttgacg aggatgcctg gcagtttctg attgcagatt atctgcgacc
    74581 ggaaaaaccc gccttccgca aatgttatga gcgtctggaa ctggcagccc gcgagcatgg
    74641 ctggagtatt ccctcccgtg ccacggcctt tcgccggatt cagcaactgg acgaggcaat
    74701 ggttgttgcc tgtcgtgaag gtgaacatgc actgatgcat ctgataccgg cacagcagcg
    74761 aactgtggaa cacctggacg ccatgcagtg gatcaacggc gacggttatc tgcataacgt
    74821 ctttgtacgc tggtttaacg gtgatgtgat ccgtccgaaa acatggttct ggcaggatgt
    74881 gaaaacccga aaaattctgg gctggcgctg cgatgtgagc gagaacattg attcaattcg
    74941 cctctcgttc atggatgttg tgactcgcta cagtatcccg gaggattttc acatcaccat
    75001 tgataacacc cgtggtgctg cgaataaatg gctgacggga ggcgcgccca atcgctaccg
    75061 ctttaaggta aaagaggacg atccaaaagg actgttttta ctgatgggtg cgaaaatgca
    75121 ctggacaagc gttgttgccg gtaaaggctg gggccaggca aaacctgttg aacgtgcttt
    75181 cggtgttggt gggcttgagg aatacgttga taagcatccg gcactggctg gcgcatatac
    75241 ggggccaaat ccgcaggcaa aacctgataa ctatggcgac cacgctgttg atgcagagct
    75301 gtttctgaaa acctttgccg aaggtgtggc gatgttcaat gccagaacag gccgtgaaac
    75361 agaaatgtgc gggggcaaac tctcgtttga tgatgttttc gagcgtgaat acgccagaac
    75421 gattgtgcgt aagccaaccg aagaacaaaa acggatgctg ttactgcctg ccgaggcggt
    75481 gaacgtttca cgcaaaggcg agtttacgct taaagttggc ggctccctta aaggcgcgaa
    75541 aaacgtttat tacaacatgg cattaatgaa tgccggagtg aaaaaagttg tggtcagatt
    75601 tgatccacag cagttacaca gcacggttta ttgag
//
LOCUS       DAJTUM010000004        72930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTUM010000004 DAJTUM010000000
VERSION     DAJTUM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO471_000314"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO471_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6241713.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO471_000315"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO471_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6241714.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO471_000316"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO471_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6241715.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO471_000317"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO471_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6241716.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO471_000318"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO471_000318"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6241717.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO471_000319"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO471_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6241718.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO471_000320"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO471_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6241719.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO471_000321"
     CDS             complement(4598..5320)
                     /locus_tag="OO471_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6241720.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO471_000322"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO471_000322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6241721.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO471_000323"
     CDS             6520..6738
                     /locus_tag="OO471_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6241722.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO471_000324"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO471_000324"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6241723.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO471_000325"
     CDS             complement(7472..7672)
                     /locus_tag="OO471_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6241724.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO471_000326"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO471_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO471_000327"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO471_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6241725.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO471_000328"
     CDS             10101..12014
                     /locus_tag="OO471_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6241726.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13035
                     /locus_tag="OO471_000329"
                     /pseudo
     CDS             12014..13035
                     /locus_tag="OO471_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            13029..13247
                     /locus_tag="OO471_000330"
     CDS             13029..13247
                     /locus_tag="OO471_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6241727.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13301..14170
                     /locus_tag="OO471_000331"
     CDS             13301..14170
                     /locus_tag="OO471_000331"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6241728.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14225..14629)
                     /locus_tag="OO471_000332"
     CDS             complement(14225..14629)
                     /locus_tag="OO471_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6241729.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14931..15563
                     /gene="crp"
                     /locus_tag="OO471_000333"
     CDS             14931..15563
                     /gene="crp"
                     /locus_tag="OO471_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6241730.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15614..17704
                     /locus_tag="OO471_000334"
     CDS             15614..17704
                     /locus_tag="OO471_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6241731.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17771..18991)
                     /gene="argD/dapC"
                     /locus_tag="OO471_000335"
     CDS             complement(17771..18991)
                     /gene="argD/dapC"
                     /locus_tag="OO471_000335"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6241732.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19077..19640)
                     /gene="pabA"
                     /locus_tag="OO471_000336"
     CDS             complement(19077..19640)
                     /gene="pabA"
                     /locus_tag="OO471_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6241733.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19672..20274)
                     /locus_tag="OO471_000337"
     CDS             complement(19672..20274)
                     /locus_tag="OO471_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6241734.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20264..20431)
                     /locus_tag="OO471_000338"
     CDS             complement(20264..20431)
                     /locus_tag="OO471_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6241735.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20536..21117)
                     /gene="ppiA"
                     /locus_tag="OO471_000339"
                     /gene_synonym="rotA"
     CDS             complement(20536..21117)
                     /gene="ppiA"
                     /locus_tag="OO471_000339"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6241736.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21388..22569
                     /gene="tsgA"
                     /locus_tag="OO471_000340"
     CDS             21388..22569
                     /gene="tsgA"
                     /locus_tag="OO471_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6241737.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="OO471_000341"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="OO471_000341"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6241738.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="OO471_000342"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="OO471_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6241739.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="OO471_000343"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="OO471_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6241740.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cobA"
                     /locus_tag="OO471_000344"
     CDS             26648..28021
                     /gene="cobA"
                     /locus_tag="OO471_000344"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6241741.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28268..28435
                     /locus_tag="OO471_000345"
     CDS             28268..28435
                     /locus_tag="OO471_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6241742.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28730..30067
                     /gene="frlA"
                     /locus_tag="OO471_000346"
                     /pseudo
     CDS             28730..30067
                     /gene="frlA"
                     /locus_tag="OO471_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30088..31110
                     /gene="frlB"
                     /locus_tag="OO471_000347"
     CDS             30088..31110
                     /gene="frlB"
                     /locus_tag="OO471_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6241743.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31160..31990
                     /gene="frlC"
                     /locus_tag="OO471_000348"
     CDS             31160..31990
                     /gene="frlC"
                     /locus_tag="OO471_000348"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6241744.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31987..32772
                     /gene="frlD"
                     /locus_tag="OO471_000349"
                     /pseudo
     CDS             31987..32772
                     /gene="frlD"
                     /locus_tag="OO471_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32872..33603
                     /gene="phoB"
                     /locus_tag="OO471_000350"
     CDS             32872..33603
                     /gene="phoB"
                     /locus_tag="OO471_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6241745.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33763..34848)
                     /locus_tag="OO471_000351"
     CDS             complement(33763..34848)
                     /locus_tag="OO471_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6241746.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34860..36164)
                     /locus_tag="OO471_000352"
     CDS             complement(34860..36164)
                     /locus_tag="OO471_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6241747.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36176..36529)
                     /locus_tag="OO471_000353"
     CDS             complement(36176..36529)
                     /locus_tag="OO471_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6241748.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36540..37418)
                     /locus_tag="OO471_000354"
     CDS             complement(36540..37418)
                     /locus_tag="OO471_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6241749.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37415..38641)
                     /locus_tag="OO471_000355"
     CDS             complement(37415..38641)
                     /locus_tag="OO471_000355"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6241750.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38642..39805)
                     /locus_tag="OO471_000356"
     CDS             complement(38642..39805)
                     /locus_tag="OO471_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6241751.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39889..40251)
                     /locus_tag="OO471_000357"
     CDS             complement(39889..40251)
                     /locus_tag="OO471_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6241752.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40268..41173)
                     /locus_tag="OO471_000358"
     CDS             complement(40268..41173)
                     /locus_tag="OO471_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241753.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41443..42447)
                     /gene="trpS"
                     /locus_tag="OO471_000359"
     CDS             complement(41443..42447)
                     /gene="trpS"
                     /locus_tag="OO471_000359"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6241754.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42440..43198)
                     /gene="gph"
                     /locus_tag="OO471_000360"
     CDS             complement(42440..43198)
                     /gene="gph"
                     /locus_tag="OO471_000360"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6241755.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43191..43868)
                     /gene="rpe"
                     /locus_tag="OO471_000361"
     CDS             complement(43191..43868)
                     /gene="rpe"
                     /locus_tag="OO471_000361"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6241756.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43886..44722)
                     /gene="dam"
                     /locus_tag="OO471_000362"
     CDS             complement(43886..44722)
                     /gene="dam"
                     /locus_tag="OO471_000362"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6241757.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44829..46121)
                     /gene="damX"
                     /locus_tag="OO471_000363"
     CDS             complement(44829..46121)
                     /gene="damX"
                     /locus_tag="OO471_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6241758.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46213..47301)
                     /gene="aroB"
                     /locus_tag="OO471_000364"
     CDS             complement(46213..47301)
                     /gene="aroB"
                     /locus_tag="OO471_000364"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6241759.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47358..47879)
                     /gene="aroK"
                     /locus_tag="OO471_000365"
     CDS             complement(47358..47879)
                     /gene="aroK"
                     /locus_tag="OO471_000365"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6241760.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48280..49518)
                     /gene="hofQ"
                     /locus_tag="OO471_000366"
     CDS             complement(48280..49518)
                     /gene="hofQ"
                     /locus_tag="OO471_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6241761.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49430..49836)
                     /gene="hofP"
                     /locus_tag="OO471_000367"
                     /pseudo
     CDS             complement(49430..49836)
                     /gene="hofP"
                     /locus_tag="OO471_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49826..50266)
                     /gene="hofO"
                     /locus_tag="OO471_000368"
     CDS             complement(49826..50266)
                     /gene="hofO"
                     /locus_tag="OO471_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6241762.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50250..50789)
                     /gene="hofN"
                     /locus_tag="OO471_000369"
     CDS             complement(50250..50789)
                     /gene="hofN"
                     /locus_tag="OO471_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6241763.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50789..51568)
                     /gene="hofM"
                     /locus_tag="OO471_000370"
     CDS             complement(50789..51568)
                     /gene="hofM"
                     /locus_tag="OO471_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6241764.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51688..54240
                     /gene="mrcA"
                     /locus_tag="OO471_000371"
     CDS             51688..54240
                     /gene="mrcA"
                     /locus_tag="OO471_000371"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6241765.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54405..54965)
                     /gene="nudE"
                     /locus_tag="OO471_000372"
     CDS             complement(54405..54965)
                     /gene="nudE"
                     /locus_tag="OO471_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6241766.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55285..57420
                     /gene="igaA"
                     /locus_tag="OO471_000373"
     CDS             55285..57420
                     /gene="igaA"
                     /locus_tag="OO471_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6241767.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57485..58153
                     /gene="yrfG"
                     /locus_tag="OO471_000374"
     CDS             57485..58153
                     /gene="yrfG"
                     /locus_tag="OO471_000374"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6241768.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58164..58565
                     /gene="hslR"
                     /locus_tag="OO471_000375"
     CDS             58164..58565
                     /gene="hslR"
                     /locus_tag="OO471_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6241769.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58590..59459
                     /gene="hslO"
                     /locus_tag="OO471_000376"
     CDS             58590..59459
                     /gene="hslO"
                     /locus_tag="OO471_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6241770.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59603..61354)
                     /locus_tag="OO471_000377"
     CDS             complement(59603..61354)
                     /locus_tag="OO471_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6241771.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61733..63355
                     /gene="pckA"
                     /locus_tag="OO471_000378"
     CDS             61733..63355
                     /gene="pckA"
                     /locus_tag="OO471_000378"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6241772.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63431..64783)
                     /gene="envZ"
                     /locus_tag="OO471_000379"
     CDS             complement(63431..64783)
                     /gene="envZ"
                     /locus_tag="OO471_000379"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6241773.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64780..65499)
                     /gene="ompR"
                     /locus_tag="OO471_000380"
     CDS             complement(64780..65499)
                     /gene="ompR"
                     /locus_tag="OO471_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6241774.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65727..66203
                     /gene="greB"
                     /locus_tag="OO471_000381"
     CDS             65727..66203
                     /gene="greB"
                     /locus_tag="OO471_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6241775.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66300..68621
                     /locus_tag="OO471_000382"
     CDS             66300..68621
                     /locus_tag="OO471_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6241776.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69059..69286
                     /gene="feoA"
                     /locus_tag="OO471_000383"
     CDS             69059..69286
                     /gene="feoA"
                     /locus_tag="OO471_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6241777.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69303..71624
                     /gene="feoB"
                     /locus_tag="OO471_000384"
     CDS             69303..71624
                     /gene="feoB"
                     /locus_tag="OO471_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6241778.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71624..71860
                     /gene="feoC"
                     /locus_tag="OO471_000385"
     CDS             71624..71860
                     /gene="feoC"
                     /locus_tag="OO471_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6241779.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72063..>72930
                     /gene="rpnA"
                     /locus_tag="OO471_000386"
     CDS             72063..>72930
                     /gene="rpnA"
                     /locus_tag="OO471_000386"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6241780.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17575 a        19760 c        18448 g        17147 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgttagtg
    12241 gtgtttcacg ggctggaagg cagtctcaat agcccttacg cccacggtct ggttgaggcg
    12301 gcgcaaaagc gcggctggct gggcgtggtg atgcattttc gcggatgcag cggtgaacca
    12361 aaccgtatgc accgcattta ccattcgggc gaaaccgaag acgccagttg gtttttacgc
    12421 tggctgcaac gcgaatttgg tcatgcgcca acggctgccg tcggctattc gctcggcggt
    12481 aatatgctgg cctgtttgct ggcaaaggaa ggcaatgatc tcccggttga tgcggcggtg
    12541 attgtctctg cgccgtttat gctggaagcc tgtagctatc atatggaaaa gggcttttcc
    12601 cgcgtttatc agcgttactt gctgaacctg ttaaaagcca atgccgcgcg caagctggca
    12661 gcctaccccg gaacgctgcc gattaatctc gcgcagttaa aatcggtacg tcgcatccgt
    12721 gaatttgacg atctaatcac cgccagaatt cacggctacg ccgacgctat cgactattat
    12781 cgtcagtgta gcgccatgcc aatgctgaac cggatcgcca aaccgacgct gattattcac
    12841 gccaaagacg atccgtttat ggatcatcag gtgatcccaa aaccggaaag tctcccctcg
    12901 caggtggagt atcaactgac tgaacatggc ggtcatgttg gctttattgg cggtacatta
    12961 cttcatccgc aaatgtggct ggagtcacgc attcctgact ggttaacaac gtatctggag
    13021 gcgaaatcat gttgattccg tggcaagacc tctcccccga aacgctggaa aatttaattg
    13081 aaagctttgt gttacgtgaa ggcaccgatt atggtgaaca tgagcgtaca cttgaacaga
    13141 aagtcgccga cgtcaaacgc cagctacagt gcggagaagc ggtgctggta tggtcggagc
    13201 tgcacgaaac ggtcaatatt atgccgcgca gtcagtttcg cgaataacct gcaaccgtcg
    13261 gcggacgaga taatagtgat agctaagtca tggagttact atgtctgcca aacatccggt
    13321 cattgcggta acaggatcca gcggcgcggg gaccaccacc accagcctcg cgtttcgtaa
    13381 aatattcgcg cagttaaatc tgcatgcagc tgaggtggaa ggcgacagtt ttcaccgtta
    13441 cacccgcccg gaaatggaca tggcgatccg caaagcgcgc gacgccgggc ggcatatcag
    13501 ctacttcggc cccgaagcga acgacttcgg cctgctggaa caaaccttca ttgaatacgg
    13561 tcagagcggc aaagggaaat ctcgcaaata tctgcatacc tacgacgaag ccgtaccctg
    13621 gaatcaggta ccgggaacat tcaccccctg gcaaccttta ccagaaccca ctgatgtact
    13681 gttttatgaa ggtttacacg gcggcgtagt cacgccacag cataacgttg cgcagcatgt
    13741 ggacttactg gtcggcgtag tgcctatcgt taaccttgag tggattcaaa aactgatccg
    13801 cgacaccagc gagcgcgggc actcacgaga agcagtgatg gactcggtag tgcgttcaat
    13861 ggaagactat atcaactaca tcacaccgca gttttcccgc acccatctta acttccagcg
    13921 cgttcccacc gtcgacactt caaacccgtt cgcggcaaaa ggtatcccgt cgctggatga
    13981 aagctttgtg gtgatccatt ttcgtaatct ggaagggatc gatttccctt ggctgctggc
    14041 gatgttgcaa ggctcattca tttcccacat caatacgtta gtggtacctg gcggcaaaat
    14101 ggggctggca atggaattaa ttatgctgcc gctggtgcaa cgattgatgg aaggaaagaa
    14161 aatcgagtaa ctttgctatt atgccggata aaatactatc cggcttcaca acgggatagt
    14221 taagtcacgc ggcaaccact tcatacgagt gagtaatatt caccgctttt tccagcatca
    14281 acgccactga acaatatttc tcggcagaga gatcaaccgc acgcgcaacc gctgcgtctt
    14341 tcaggtcgcg accggtgacg ataaaatgca gattaatgtg cgtaaacagg cgtggtgcct
    14401 cttcgcggcg ttcagaggtc aattttactt cacaatcgac cacatcctga cgcccttttt
    14461 gcaggatcga aaccacatcg atggcactgc aaccacccgc cgccatcaac accatttcca
    14521 tcggacttgg tgctttatcg cctgagttgc cgtccattaa aatctggtga ccagaggcgg
    14581 attcgcccag aaaagttaac ccttcgaccc acttcactcg cgcttgcatt tttgctactc
    14641 cactgcgtca attttcctga cagagtacgc gtactaacca aatcgcgcaa cggaaggcga
    14701 cctgggtcat gctgaagcga gacaccagga gacacaaagc gaaagctatg ctaaaacagt
    14761 caggatgcta cagtaataca ttgatgtact gcatgtatgc aaaggacgtc acattaccgt
    14821 gcagtacagt tgatagcccc ttcccaggta gcgggaagca tatttcggca atccagagac
    14881 agcggcgtta tctggctctg gagaaagctt ataacagagg ataaccgcgc atggtgcttg
    14941 gcaaaccgca aacagacccg actctcgaat ggttcttgtc tcattgccac attcataagt
    15001 acccatccaa gagcacgctt attcaccagg gtgaaaaagc ggaaacgctg tactacatcg
    15061 ttaaaggctc tgtggcagtg ctgatcaaag acgaagaggg taaagaaatg atcctctcct
    15121 atctgaatca gggtgatttt attggcgaac tgggcctgtt tgaagagggc caggaacgta
    15181 gcgcatgggt acgtgcgaaa accgcctgtg aagtggctga aatttcgtac aaaaaatttc
    15241 gccaattgat tcaggtaaac ccggacattc tgatgcgtct gtctgcacag atggcgcgtc
    15301 gtctgcaagt cacttcagag aaagtgggca acctggcgtt cctcgacgtg acgggccgca
    15361 ttgcacagac tctgctgaac ctggcaaaac aaccagatgc tatgactcac ccggacggta
    15421 tgcaaatcaa aattacccgt caggaaatcg gtcagattgt cggctgttct cgtgaaaccg
    15481 tgggacgcat tctgaagatg ctggaagatc agaacctgat ctccgcacac ggtaaaacca
    15541 tcgtcgttta cggcactcgt taatcccgtc ggagtggcgc gttacctggt agcgcgccat
    15601 tttgtttccc ccgatgtggc gcagactgat ttatcacccc gatatcaact atgcacttcg
    15661 acaaacgctg gtgctatgtt tgcccgtggc cgttgggtta atgcttggcg aattacgatt
    15721 cggtctgctc ttctccctcg ttcctgcctg ttgcaatatt gcgggccttg atacgcctca
    15781 taaacgtttt ttcaaacact taatcattgg tgcgtcgctg tttgccacct gtagcttact
    15841 gacacagctg ctactggtaa aagatgttcc cctgcccttt ttgctgaccg gattaacgct
    15901 ggtacttggc gtcactgctg agctggggcc attacacgca aaattgcttc ctgcatcgct
    15961 gctcgccgcc atttttaccc tcagtctggc gggatacatg ccggtctggg aaccattgct
    16021 catctatgcg ttgggcactc tctggtacgg attgtttaac tggttttggt tctggatctg
    16081 gcgcgaacaa ccgctgcgcg agtcactaag tctgctgtac cgtgaactgg cagattattg
    16141 tgaagccaaa tacagcctgc ttacccagca caccgatcct gaaaaagcgc tgccaccgct
    16201 gctggtgcgc cagcaaaaag cggtcgattt aattactcag tgctatcagc aaatgcatat
    16261 gctttccgcg caaaacaata ccgattacaa gcggatgctg cgtattttcc aggaggcgct
    16321 ggatttgcag gaacatattt cggtcagttt gcatcagccg gaagaggtgc aaaaactggt
    16381 cgagcgtagc catgcggaag aagttatccg ctggaatgcg caaaccgtcg ccgctcgcct
    16441 gcgcgtgctg gctgatgaca ttctttacca tcgcctgcca acgcgtttta cgatggaaaa
    16501 gcaaattggc gcactggaaa aaatcgcccg ccagcatccg gataatccgg ttgggcaatt
    16561 ctgctactgg catttcagcc gcatcgcccg cgtgctgcgc acccaaaaac cgctctatgc
    16621 ccgtgactta ctggccgata aacagcggcg aatgccgttg cttccggcac tgaaaagtta
    16681 tctgtcacta aagtctccgg cgctacgcaa tgccggacga ctcagtgtga tgttaagcgt
    16741 tgccagcctg atgggcaccg tgctgcatct gccgaagtcg tactggatcc tgatgacggt
    16801 attgctggtg acacaaaatg gctatggcgc aacccgtctg aggattgtga atcgctccgt
    16861 gggaaccgtg gtcgggttaa tcattgcggg cgtagcgctg cactttaaaa ttcccgaaag
    16921 ttacaccctg acgttgatgc tgattaccac cctcgccagc tacctgatat tgcgcaaaaa
    16981 ctacggctgg gcaacggtcg gttttactat taccgcagtg tataccctgc aactgttgtg
    17041 gctgaacggt gagcagtaca tccttccacg tcttatcgat accattattg gttgtttgat
    17101 tgctttcggt ggtaccgtct ggctgtggcc gcagtggcag agcgggttat tgcgtaaaaa
    17161 cgcccatgat gctttagaag cctatcagga agcgattcgc ttgattctga gcgaggaccc
    17221 gcaaccaacg ccactggcct ggcagagaat gcgggtaaat caggcgcata acactctgta
    17281 taactcattg aatcaggcga tgcaggaacc ggcattcaac agccattatc tggcagatat
    17341 gaaactgtgg gtaacgcaca gccagtttat tgttgagcat attaatgcca tgaccacgct
    17401 ggcgcgggaa caccgggcat tgccacctga actggcacaa gagtatttac agtcttgtga
    17461 aatcgccatt cagcgttgtc agcagcgact ggagtatgac gaaccgggta gttctggcga
    17521 tgccaatatc atggatgcgc cggagatgca gccgcacgaa ggcgcggcag gtacgctgga
    17581 gcagcattta cagcgggtta ttggccatct gaacaccatg cacaccattt cgtcgatggc
    17641 atggcgtcag cgaccgcatc acgggatttg gctgagtcgc aagttgcggg attcgaaggc
    17701 gtaatacagg ctaaaccatc ggataaggca ttcacgccgc atccgacata ttttgcctga
    17761 tacgtcttat ttacgcccca accaccttcg ccaccgcgtg ggcgaaacgt tgcatccctt
    17821 catcgatatc cgcatcttcc accaccagcg acggtgctaa acgcatcaca tccggtccgg
    17881 cattcagcac cattacgcca acctctgcac ccgcatacag gaaatcacgc gcccgacctt
    17941 tgtagtgtgg tttcagctcc gcgccaatta acagccccat accgcgaata tcgctaaaca
    18001 catcgtactg ctgatcgatc ttctgcaaat ggtcaacaaa acgctggcgt ttcgcctgaa
    18061 tgccttccag cacttcaggg gtattgatga tatcaaacgc cgcgcccgct actgcacagg
    18121 ccagaggatt accgccgtag gtggaaccgt gagaaccagg atgaaacgca gaagcaattt
    18181 ccgccgtggt cagcatggcg ctaatcggga agccgccgcc taacgctttc gcagaagtca
    18241 gaatatccgg cgtcacaccg tagtacctgt aagcaaacaa atcgccagtc cgccccatcc
    18301 cgcactgcac ttcatcaaac accaataatg cctgatgttg atcgcacagc tcgcgcaagc
    18361 cctgcaaaaa ctctggcgtc gctgccgtca caccgccctc gccctggatc ggctcaacca
    18421 ccaccgcaca ggtgtgatca tccatcaccg ctttcactgc atggagatcg ttaaagggaa
    18481 cgtggatgat gtctgccggt ttcggcccaa agccgtcgga atattttggc tgcccaccca
    18541 ccgaaacggt aaacagcgag cgaccatgaa aagcgttatg gaaggcaata attttggttt
    18601 tgaacgggct atgacgcaca caggcgtaat ggcgtgccag tttaaaggcg gtttcgttag
    18661 cttccgtgcc ggagttcata aacaccacgc gttcggcaaa cgtcgcctca atcagtttgc
    18721 gcccaagacg cagcgccggt tcattggtga aaacgttact gatatgccac agagtttcgc
    18781 cctgggtttt taacgcgttc accagcgcag gatggcaatg gcccaacgcc gtaactgcaa
    18841 tgccacccgc gaaatcgaca tactccttgc cttgctgatc ccagattcgg ctgccctgac
    18901 cttttaccgg aataaactct gccggagcat aaatcggcag gatcacttca tcgaaagtcg
    18961 cgcgtgtaat tgctgtttgt tcaattgcca tctcatgatc accctgttac gcataaataa
    19021 atgtgaaatt ataatcacaa aatatgcata aaaaatcact gaatggcaat cggaaatcag
    19081 agatgcagga aattagccag cagttgatgt ccttgttcgc taagaatact ttctggatgg
    19141 aactgcacac cttccagatc ccactggcga tggcgaatcc ccataatctc gcgggtttcg
    19201 ctccaggccg tcacgtcaaa gcacgctggt aatgagtcag gttccaccac cagcgaatgg
    19261 tagcgtgtca cggtaagtgg atttgccagc ccccgaaata cgccctcacc gctatgtgta
    19321 atcggcgagg ttttgccgtg catgaccttt gcggcgcgca caactttgcc gccaaatgcc
    19381 tgcgccattg cctgatgacc gaggcagacg ccaagaatcg gcaagcgccc ggcatagtgg
    19441 cgaataacgt caagggagat cccggcttca tctggcgtac aggggccagg tgagatgaca
    19501 attttttgtg gtttaagggc gtcgatatcc gccagcgtca acgcatcgtt gcgcttaacc
    19561 agcacatccg cccccagttc acaaaagtac tggtagaggt tccaggtaaa agaatcgtag
    19621 ttatctataa gcaggatcat ggcggctccg gtacgaaaga accgcgctat tttactcaga
    19681 ttccccggct tcgcttacca ctttgctaaa tatcatctgc agtgcggtga gatcccccat
    19741 tgccccactc tgatttgcct gattccaggc ctctttttcg ataccctgcc agctcagttg
    19801 atatccggca tgaatcgcca gttgctcgaa gaagatccgc tgtgccagac cacttcccac
    19861 ccggaaggga tgcagcacgt tgatttcaca atagtaatgc gccagccgct cgacgaactt
    19921 cgctttctcc aggccaacca gataaccttc ttcctccaga tcctgcatca gggcattgcc
    19981 ctctttttcg atataagcaa agtggcagaa cggcgtatcg ccctgataaa tatcaacttc
    20041 acgcagttgc cctgcccagt cgaaaatatc ctggtacagc tggcgatgga tagtgcgcaa
    20101 atgcggtaaa ccgcgcacca gcggaccaag ctcaatggtc gcagcacgca gcgccgtcat
    20161 ttcgtaagcg gcctgttcca gccgctgctg ctggcggatg ttcagccggt tacgcatgat
    20221 atcaaggcca ggataaagat acagatcgcg cccttcgccg aatttatcgc tcatagtgcc
    20281 tcctcagctc ttcaaggcgc gctaaagcct ctgcggcagt aagagtgact aaaggcatct
    20341 cgacgccttc aagacggcga ctggcctgga aattacgatt acgctgaagc tcccagagac
    20401 gggacttttg cttatcggtg agtttcttca cttgatgcct ccctgaacgt gtccatttgc
    20461 cacaagtata agcggcaaat ccgggttacg ccgggagagc agaagcgcgg gcaagattgc
    20521 ccgcgcgaga aatcattacg gcaggacttt agcggaaagg ataactaccg gttttgacgg
    20581 cacattctgg tacggaccaa catcatgagt cggcacctgg gaaatcttat cggcaacgtc
    20641 catgcctttc accactttac caaataccgc gtaaccgaaa tcacgctgac catggtcaag
    20701 gaaggcgtta tcggcaacgt tgataaagaa ctggctggtg gcgctgtctt tgtcagcggt
    20761 acgcgccatc gcgatggtgc cacgcgtgtt gcgcaggccg ttatcggctt catttttgat
    20821 tggcgggttt ggttttttct gctgcatctg ctcggtgaaa ccgccgccct gaatcataaa
    20881 gccaggaatg acgcggtgaa aggtagtgtt gttataaaaa ccgctgttca cataatcgac
    20941 aaagttttgc acagacactg gcgctttttg tttatccagc tccagttcga tgttaccagc
    21001 tgaggttgtc aacaatacgt gcgggtcccc tttcgctgcc attgctgcgg gagaaagagc
    21061 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21121 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21181 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21241 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21301 acatttaaac agatcataaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21361 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21421 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21481 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21541 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21601 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21661 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21721 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21781 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21841 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21901 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacgt
    21961 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28261 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28321 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28381 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28441 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28501 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28561 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28621 acatcacata acataatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat
    28681 ggctgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28741 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28801 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28861 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28921 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    28981 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29041 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29101 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29161 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29221 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29281 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29341 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29401 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29461 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29521 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggctctttgt
    29581 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29641 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29701 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29761 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29821 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29881 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    29941 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg ccatcgttat
    30001 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30061 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30121 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30181 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30241 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30301 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30361 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30421 ctggagctgg gccgggcctg cggagcactc actgcggcgt tcaccaaacg cgcggacagc
    30481 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30541 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30601 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30661 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30721 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30781 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30841 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30901 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    30961 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31021 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31081 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31141 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31201 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31261 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31321 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31381 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31441 ggcgatagat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31501 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31561 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31621 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31681 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31741 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31801 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31861 tattattgat cggggctatg agggttactc tacggtggag ctggtgacga tgtatatgaa
    31921 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    31981 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32041 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32101 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32161 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32221 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32281 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32341 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32401 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32461 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32521 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32581 attgcctagg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32641 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32701 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32761 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32821 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32881 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    32941 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33001 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33061 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33121 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaagtc gacgaaagag
    33181 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33241 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33301 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33361 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33421 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33481 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33541 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33601 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33661 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
    33721 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33781 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33841 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33901 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    33961 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34021 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34081 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34141 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34201 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34261 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34321 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34381 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34441 atcaggttgt tgctcgtcga cgacctgctt cagtgccgac aggtcattga aatcagcagt
    34501 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34561 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc
    34621 agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg
    34681 ctccacccgt tgggtaatac gcggttgatt cagacttgga gttaacccta aatcaccgcc
    34741 agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt
    34801 ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag gtgccttcct
    34861 taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt
    34921 actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag
    34981 taaatggcaa ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc
    35041 gaaccgacca gcagcgccac ttccatcagc atattcatgg cgttacggat gttatccgac
    35101 gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact
    35161 tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag
    35221 cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg
    35281 gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta
    35341 atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag
    35401 atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc
    35461 acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa
    35521 agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc
    35581 cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa
    35641 tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc
    35701 cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc
    35761 gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta
    35821 actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc
    35881 aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg
    35941 agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga
    36001 ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg
    36061 atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gcgccagaag cgatgtcata
    36121 cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt
    36181 tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg
    36241 ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta atgcctggtt
    36301 tggcaatggt gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac aatgccgcgc
    36361 ccgcaccggt attacacgcg ccaatgtaat aatctagttg cccggatttc accttcattg
    36421 ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc gcttcaatgg
    36481 tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg agtctgtcct
    36541 tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc ctgactgaat
    36601 cctgactggc gcaattgcgg aataaaggtg gtcaataaat agtcatagcc ataaccacca
    36661 ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg gttcagcaac
    36721 ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta gtaactgttc
    36781 ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa aatgttgtcg
    36841 aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac tccgtgggct
    36901 tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt ggagatcggg
    36961 cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc cagcggcgta
    37021 atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc cgtgccatcg
    37081 ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct gcgggtcacc
    37141 acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac gttgatcccg
    37201 gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg gttggtcatc
    37261 tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca aatgaacgca
    37321 tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat atgcagatgc
    37381 tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct gcaaaccgga
    37441 gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat tcacacacgg
    37501 tagctcccac atcagaaagc gtggtgcgca caccgagctg cgtggcgacc agcccttgct
    37561 gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg gtcggatcgt
    37621 taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca acaagccggg
    37681 caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct tctgcatgac
    37741 cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgg gtgttaaatt
    37801 cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag ctcacaccat
    37861 aaggattgct gacgatatct gccaccttac ccaccagcac ggttggcacg cccgcttcat
    37921 acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc acgacctgga
    37981 agccgttgtc ataagcgcca gaacgcggcg cattaatacc aataaagcgc ccttctttgc
    38041 tttctgcggc atcgagaata cgctgactgt tggttaacag gccgccaaat gtaatgaccc
    38101 gaccgacctg tacctggtca cgcacgatac gaccaatttt gattgcgtcg tcaaaagaga
    38161 tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg agattatcgc
    38221 caatcgcaac cgcctgattg accgacagaa attgcagatc atcgccacgg cgctccacct
    38281 gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga aaaggcatcc
    38341 gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag gtatcgccac
    38401 cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc atatcgcctg
    38461 gcgcataacc caatgtgttg attagcccca gcttctccag cgttggtagc tgcaaatgcg
    38521 gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcggg cgcaccagcg
    38581 tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca aatcgcgcca
    38641 tttatgcctc cagagggttg ccgaggctgt cataacgacc aactatttct ggttcgccgc
    38701 gctgaatacc ggacaccagc accacatcgc tacgggtgac aaaaatctgc gtgcgaaaac
    38761 agagcaccac tgcactgctc accggatact cgcctgccag cggcagggtg tagtcgatac
    38821 tgctgtcatc cacagttttg agattggttt cagtaatctt ttgattttct ggcgtaaaca
    38881 ccagcgcatg ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc
    38941 cacggaaatg atgggagatt tcacttaacc agagcatcgc gatacgttca ggctgatcgc
    39001 cctgctggtt tgccggaata gtaccggtca gcgcatgacc gggttcggca tgagtcgcac
    39061 cgtattccgc cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg ttcagttgct
    39121 caatcgcaat accagatttc gccagttgat cccgtgcttg taccagcgtg tgaagattcg
    39181 gtgttggcaa aactttccca gcagcctcat cccaaagcag gcaagggaaa tgggttagtc
    39241 cggctaaatg cagccccggc agtttcttaa tttcagcaat cacctcgtgc agcgaatgct
    39301 gtacaaaacc gctctcctgc cccggataaa gaaaatcatc gtcgctataa actttgagca
    39361 gcacaggctg gacccgtccg gccttaaccg ctgccgcaga aacttcccgc gctttgtcga
    39421 gagtaaacac ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc tgatgacaag
    39481 ggatttgtac cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc atgactcgcg
    39541 cctctttgta atccaccgcc acaatgccgc tgtagcctaa tgccagcaat ttttccgcca
    39601 gccacggatt acgaccaaat tgcttggtca tcagatacag ttcaatgccg taaagccgcg
    39661 ccgtctcaat gagccgctta ccgttttcca gtatctgatc cacgtcgatc acccagctgt
    39721 ccggggcgat tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc gccgggttct
    39781 ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt ctgttccttg aattaaatat
    39841 tcattttttt gaatatttag gttgtgcgac gtggatttgt cgggagcttc aaacctcttc
    39901 gttagcagcc atccataatc cataaagatt ggccagaaaa tagccttctt cacacgggtt
    39961 aacttccaga gcgaattcct tcaacaactc ctgatgcaat tgcacaacgg cttgccagtg
    40021 gctggattgc gccagttccg ccagcaactc gttatccagc ggctctattt cttcaccacg
    40081 gcgactgcgc atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg
    40141 caccggcaga tggcactctt tttccagtac gttgacgacc tgcatcatgc ctttgcagac
    40201 gtccttatca ataacgcctg cctcgcaaag caggttgagt ctggtttcca tatcagatgc
    40261 ctttcgttta ataactgggt tcctgttccc cactcaccac ccgttgttga tgggcaagca
    40321 gggcgtgttt atctacaaca gcacgcagca attgttgcat tgggtaatca tcgactcgcg
    40381 tcaggaccac ggcttcggtg gcctgtaaaa atcgcggatc gtctgttagc ggcgtcgcct
    40441 ctaatcccag catggtcagt tcgttttccg ccaccacgtt ccagatcacc gcatcgacat
    40501 cgcctttaac aatgcgttgt aaactctcgt gataagagag atcgactcgt tccacatcac
    40561 tatcgccaaa aaagacatcg gtcatgattt tctgatccgc cgaacgacta tcgagaccta
    40621 ctcgcttaac atttccagac tcacctttgc ggcatatcag ttgatgttca ccaacatacg
    40681 tatgcgggcc aagctcgagg gcgatgcaca aattgttttg gctgagataa ctttccgccg
    40741 ccaggcggga aactactgcc atgtcataga caccgttaag taaacactcc acgcgaatat
    40801 ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc aaactgggct ttcaatccgc
    40861 tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc acgttgttga
    40921 tatccacatg actcagcaat gctttgttat ccatctcgac aagataactg ccattgcgcc
    40981 cacggcgttc aatccgtatc gctccgcttg attccagcgt tttcaacgcg gcctgcgtca
    41041 ggccaaccga tgaacggcat tcagttgcca gctcatctat ggttttcagt cgattaccgc
    41101 atttttcacc caacaaataa cgggccagtg tcgtgatgac gacgccttct tttttgataa
    41161 acgttcgacg cataatatat tttcaataaa atgaatattt atatcttcat taaaatgaag
    41221 ataagtggcg acgacgagat gtggaaacgg cgaggcgctt cacatttttt cgggttgttg
    41281 cgaaaagagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat
    41341 tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc
    41401 ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa
    41461 accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc
    41521 gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc
    41581 ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata
    41641 catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta ccgcagaaag
    41701 gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg
    41761 ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag
    41821 caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag
    41881 cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc
    41941 gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg
    42001 gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac
    42061 cggatagcca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa
    42121 ctgcgtcatg cgactcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg
    42181 cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca
    42241 agccagatac aaggccagcg tatccaacgt cgctttacgc agcttctgtg catcctggcg
    42301 cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt
    42361 tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc
    42421 gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc
    42481 ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga
    42541 tagcctcgcc gtagttgtat ccgtaggtta agccaactga tgggcaacct gccgcttttg
    42601 ccgcctgaat atcattccgt gagtcgccga caaacagcat ctgttgtggg gcaattccca
    42661 tccgctcagc caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc
    42721 cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga
    42781 acggcgtcgg tttgttggtg accaagccta gcggcaggcc tttagcctgc aacgcgccca
    42841 acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc
    42901 gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg
    42961 gcggtttccc cattgtttta cgcagagtcg cacgttcctg acgcgcccag gtcaatgcgc
    43021 gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga
    43081 cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagacca ggagcactgt
    43141 cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga
    43201 cttacctttg ccagttcact gcgcatttca tcaatgactt ttttatagtc tggctggtcg
    43261 aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg
    43321 ttcaccttca cgccaccgtc cacttccaga cgaatgtcaa agccagactc gtcgatacgg
    43381 cgacgtactt cgcgcagttt atccagtgtt tgaggaatga aagactgacc gccgaaacca
    43441 gggttgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc
    43501 agaggtgtcg ccgggttaaa taccagaccc gctttacagc cattttcttt aatcagttgc
    43561 agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg
    43621 gcagcgaaat caggcacaat gcgatcgacg ggtttcacca tcagatgtac gtcgataggg
    43681 gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt cagattggga
    43741 acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt
    43801 gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac
    43861 tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt
    43921 acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc
    43981 gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt
    44041 gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat
    44101 gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag
    44161 acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgctccatgc
    44221 tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca
    44281 actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc
    44341 gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg
    44401 cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg
    44461 cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca
    44521 tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag
    44581 aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc
    44641 ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca
    44701 aaaaagcgcg atttttcttc atgccgatta actaattaca ccttctccgg ctgtggagaa
    44761 agctccgaca gcatccagcg cttcaggcag ctcccgcaac gataaaccat tgcgggagat
    44821 aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg
    44881 gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac
    44941 acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag
    45001 tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc
    45061 agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc
    45121 ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc
    45181 gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc
    45241 acaggggcag ctggttcagg ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc
    45301 gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg aactgggcgc
    45361 gtagtggacg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc attgccattg
    45421 cgaacaggcg caaccgttgc gggttcagtc ggcaacgtgg aattgaccgc cacattgttc
    45481 agctgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca
    45541 cgttgttgac catccgttgc caccggggtt tgcccttgag tcggcgtaga agagatcggt
    45601 ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcacg
    45661 ccattcgcct gatcggtcgc attgccagca agatcaatac tcttctcgcc agacgcggtt
    45721 tgatcgctgg aagtggtcga gggggctttt agcgcagaac cgataccgat gatcaacagc
    45781 agtagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg tttactggct
    45841 gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt ttcatcttcg
    45901 gattcgtcaa tttcttcttc gatttccggt tcctcattgc gctctttttg cgcacgagtc
    45961 ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca
    46021 cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg
    46081 ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg
    46141 ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga
    46201 cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga
    46261 aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc
    46321 cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg acatttcgcg
    46381 cggcccattg accggtaacc cagcccgcgt gagcagggtt ataatacgct gcgtttcggc
    46441 agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcgac
    46501 cgcttcacca tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt
    46561 gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc
    46621 tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa
    46681 caacgcatcc agattctctt ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta
    46741 tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc
    46801 gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg
    46861 atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg
    46921 gacttgaatg aaacgaacac cgcgctgata actcgccgcc gcgaagccgg tcagatcgcc
    46981 cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa
    47041 cgccgtaaag acggtatcga gtacagctag gcttttatac tgctcgccgt cagggaggat
    47101 aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag
    47161 aggagccagg gtttcgctgg tgaccaacat gacctgctcg cccgatttca gcggtaagaa
    47221 tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag
    47281 agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag
    47341 tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcaaccact
    47401 ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg
    47461 cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg
    47521 cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg
    47581 acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga gccgccgcca
    47641 gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg
    47701 cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc
    47761 tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca
    47821 atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt
    47881 ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag agagcgccgc
    47941 aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata
    48001 ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa
    48061 gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa
    48121 agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc agcgaagagc
    48181 accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa
    48241 acacgttcag cataaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg
    48301 tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa
    48361 ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg atttattttt
    48421 acgggtaaaa atgccgccca gcgccaacgt ttctccgctt tttacctcga cctgcgtttc
    48481 gatctcctgc ttatcaatcg ccagcacttc gccatcggcc tgctgtagca cctgccccgg
    48541 aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt
    48601 gacctccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct
    48661 ggaaacctga tatggaattt cgctctcctg tttaatgctg gcaggctgaa gatgtgaggc
    48721 cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg cggaaagctc
    48781 aagatccagc aagcgtccgt tgatgcgccc aatgttaaaa ccgacatgcg ttgtcgccgt
    48841 cgctacggag aggtcgctac caagcgtggt gacttgccca acgccaccag cgtgttgcgc
    48901 atcggccagc gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat
    48961 atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc actgttcaag
    49021 cgcgcttaac gccgttttgt tatctcgtag caaaaggcga ttggtgcgtt tatcgacggt
    49081 catactccct ttggcactca gtagcttctc ccccgctttc gccagttctc ccgcgtcggc
    49141 gtattgcagg gttatactgc gattttccag cggcagattt gcctgcgccc gcgcctgctc
    49201 cgcctcctgg cgggcgatat tgtcattctg ccaggcaatg gaatgcactg agagaatgtt
    49261 gccttcctgc cgcgttatca gtccggcgct tttcactaca gtttgtagtg cctgcttcca
    49321 gggaacatct gttagatgta acgacaccgt accgctgacg tctggcgaca cgaccaggtt
    49381 caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat
    49441 cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca acagtagtgc
    49501 ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccattgt ggcggttcgc
    49561 agtttgtccc ggtacccagc gttagtacgt ctggcgtcag ctgtaaaatt gtccagccgt
    49621 tttccagcac atcgttttgc tgcacccgtc gccatttctt ttgcccgtct tttattacac
    49681 cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta agttcgctaa
    49741 tccggcatat agatcttccg gcgatttaaa agggtcacgc ataccggtta aaaggcacaa
    49801 tgcaatacct gccaacaacc agcgtttaac cctcatgcgg catctccagt tgtagcgtga
    49861 acaaaagatc atcaccttcc acgcttaacg aaaaacggct cacgctgacg ttgcgctctg
    49921 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcacctc
    49981 cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg
    50041 aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc taccaggcga tacagtgcgg
    50101 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50161 tcgatgatag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50221 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50281 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50341 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50401 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50461 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50521 tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt
    50581 ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct
    50641 atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50701 agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc
    50761 cagggcaaaa aattaattgg cggattcatc agtacgcctt cccaagcgcc agccccagcg
    50821 cgatggcaaa gtctccacca ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50881 aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc gggatcaacg gataacgctg
    50941 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag ctatagcgcg
    51001 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51061 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcactaaca tgaacacgca
    51121 acctttctgc cagcgtaagc agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51181 aagcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca
    51241 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg
    51301 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51361 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa
    51421 caatccgccc atctttgata atgtcgtgcg ccagcggcaa ccgccaccag cgttgcaaaa
    51481 agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc ttcttgctgt tgtaaatgca
    51541 aaccaatttg ccagatctta aatgtcattg tgatgatctc cttatcaccc gtcactctga
    51601 cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc
    51661 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51721 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51781 caactgccgg atgtggtgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51841 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51901 gatcaaatcc caccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51961 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    52021 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa tttcttcctc
    52081 agtccagaac gcacgatgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52141 cagttgctga cgaaagacga aatcctcgag ctttatctga acaagattta ccttggttac
    52201 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52261 acgctgaacg aaatggcggt gatagccggg ttgccgaaag cgccttccac ctttaatccg
    52321 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52381 gaagggtata tcacccagca acagttcgat cagacacgca ctgaggcgat taacgctaat
    52441 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52501 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52561 acccgcaaag tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct ggactacgac
    52621 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52681 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52741 gccgcagtca ccagcgccaa tcctcaggga gcgacggcga tgctggcgga cgggtcgacc
    52801 gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52861 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52921 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52981 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    53041 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53101 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53161 tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat
    53221 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53281 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53341 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53401 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctagtgga cccgtggttt
    53461 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53521 tgcccggaat gcgatattcc agtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53581 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53641 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53701 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53761 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53821 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53881 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53941 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    54001 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54061 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54121 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54181 gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga
    54241 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54301 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54361 ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54421 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54481 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54541 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54601 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54661 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54721 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54781 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54841 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54901 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54961 ttcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    55021 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    55081 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga
    55141 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taagctccac
    55201 gcttgccgat agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga
    55261 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55321 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55381 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55441 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacggaagct
    55501 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55561 cacgctatca cacgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55621 gattcggtag aagtccacct gccccccttc tgggaacagt acatcaacga tgagaatacc
    55681 gttgaactga ttcataccga ttcgctgccg ttggttattt cactcaacgg tcatacgctg
    55741 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55801 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55861 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55921 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55981 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    56041 tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56101 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56161 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56221 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56281 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct agtgctgttt
    56341 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56401 ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56461 ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56521 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac
    56581 gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56641 gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta
    56701 cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56761 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56821 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56881 gatggcgtga atgtgttatt acgcccggtg agtgctgaat cgctggataa cctggtggca
    56941 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    57001 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57061 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57121 ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc
    57181 actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg
    57241 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57301 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc
    57361 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57421 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57481 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57541 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57601 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57661 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57721 ggatatctgt gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat
    57781 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca
    57841 tccgcacaac ctggcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt
    57901 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57961 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    58021 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58081 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58141 ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt cgactggata
    58201 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58261 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58321 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58381 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58441 agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58501 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58561 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58621 tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58681 tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58741 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg
    58801 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58861 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58921 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc
    58981 tggaaggtga taccctggcg gcctgcctag aagattactt tatgcgttct gaacagctgc
    59041 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59101 tgttgcaggt aatgcctgcg caaaataccc aacaggacga ctttgaccac ctggcgacgc
    59161 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59221 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59281 cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata
    59341 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59401 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgtaag
    59461 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgact taatggcctg
    59521 atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa ttattttggt cagatacagc
    59581 gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59641 ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga
    59701 gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt
    59761 ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc
    59821 gctccgcctg accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa
    59881 tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt
    59941 taacagaacc aggggcaatt aacacatttt cggcgaatac tttttccgat acctgttcct
    60001 gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca
    60061 taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt
    60121 cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat
    60181 gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca
    60241 ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc
    60301 cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac accagctccg
    60361 ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacc
    60421 cccaggtggc gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac
    60481 gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga
    60541 tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg
    60601 cgctcaggcc cgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg
    60661 gcagcaaccc cgtggcgatt agcgtgaaca acttttgaat agagtcgatt aaacgtgact
    60721 gtgtacgcgc gaggatcacc gccagtgcgg taaccagacc aaatgtaaga tacataaacc
    60781 agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcac
    60841 cccacagtaa aagcaccagc cacgtcaggc cattggagag aaaaatcacc agtaatataa
    60901 gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcggatac
    60961 gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa
    61021 aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc
    61081 aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct
    61141 gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc
    61201 cataaacaat ccaactgtga ttttttccgg caagccaggc gataagcagg tagcaaacca
    61261 gcccctgaag taatccggta gcaatcatcc cctgaagtaa tccggtagca atcatccccc
    61321 agcgtgttgc cggtgaaagt tcaacgttat ccatgttggt tatccagaat caaaaggtgg
    61381 gttaattatc gcatccgggc agtagtattt tgcttttttc agaaaataat caaaaaaagt
    61441 tagcgtggtg aatcgatact ttaccggttg aatttgcatc aatttcattc aggaatgcga
    61501 ttccactcac aatattcccg ccatataaac caagatttaa ccttttgaga acattttcca
    61561 cacctaaaat gctatttctg cgataatagc aaccgtttcg tgacaggaat cacggagttt
    61621 tttgtcaaat atgaatttct ccagatacgt aaatctatga gccttgtcgc ggttaacacc
    61681 cccaaaaaga ctttactatt caggcaatac atattggcta aggagcagtg aaatgcgcgt
    61741 taacaatggt ttgcccccgc aagaactcga ggcttatggt atcagtgacg tacatgatat
    61801 cgtttacaac ccaagctacg acctgctgta tcaggaagag ctcgatccga gcctgacagg
    61861 ttatgagcgc ggggtgttaa ctaatctggg tgccgttgcc gtcgataccg ggatcttcac
    61921 cggtcgttca ccaaaagata agtatatcgt ccgtgacgat accactcgcg atactttctg
    61981 gtgggcagac aaaggcaaag gtaagaacga caacaaacct ctctctccgg aaacctggca
    62041 gcatctgaaa ggcctggtga ccaggcagct ttccggcaaa cgtctgttcg ttgtcgacgc
    62101 tttctgtggt gcgaacccgg atactcgtct ttccgtccgt ttcatcaccg aagtggcctg
    62161 gcaggcgcat tttgtcaaaa acatgtttat tcgcccgagc gatgaagagc tggcaggttt
    62221 caaaccagac tttatcgtta tgaacggcgc gaagtgcact aacccgcagt ggaaagaaca
    62281 gggtctgaac tccgaaaact tcgtggcgtt taacctgacc gagcgcatgc agctgattgg
    62341 cggcacctgg tacggcggcg aaatgaagaa agggatgttc tcgatgatga actacctgct
    62401 gccgctgaaa ggtatcgctt ctatgcactg ctccgccaac gttggcgaga aaggcgatgt
    62461 tgcggtgttc ttcggccttt ccggcaccgg taaaaccacc ctttccaccg acccgaaacg
    62521 ccgcctgatt ggcgatgacg aacacggctg ggacgatgac ggcgtgttta atttcgaagg
    62581 cggctgctac gcaaaaacta tcaagctgtc gaaagaagcg gaacctgaaa tctacaacgc
    62641 tatccgtcgt gatgcgctgc tggaaaacgt caccgtgcgt gaagatggca ctatcgactt
    62701 tgatgatggt tcaaaaaccg agaacacccg cgtttcttat ccaatctatc acatcgataa
    62761 catcgttaag ccggtttcca aagcgggcca cgcgactaag gttatcttcc tgactgctga
    62821 tgctttcggc gtgttgccgc cggtttctcg cctgactgcc gatcaaaccc agtatcactt
    62881 cctctctggc ttcaccgcca aactggccgg tactgagcgt ggcatcaccg aaccgacgcc
    62941 aaccttctcc gcttgcttcg gcgcggcgtt cctgtcgctg cacccgactc agtacgcaga
    63001 agtgctggtg aaacgtatgc aggcggcggg cgcgcaggct tatctggtta acactggctg
    63061 gaacggcact ggcaaacgta tctcgattaa agatacccgc gccattatcg acgccatcct
    63121 caacggttcg ctggataatg cagaaacctt cactctgccg atgtttaacc tggcgatccc
    63181 aaccgaactg ccgggcgtag acacgaagat tctcgatccg cgtaacacct acgcttctcc
    63241 ggaacagtgg caggaaaaag ccgaaaccct ggcgaaactg tttatcgaca acttcgataa
    63301 atacaccgac acccctgcgg gtgccgcgct ggtagcggct ggtccgaaac tgtaatgatt
    63361 tgaagctgga gaatatctat ccagtatctt atagaaagca aaacgggagg caccttcgcc
    63421 tcccgtttat ttacccttct tttgtcgtgc cctgcgcccg cgttaccggc actggcagcc
    63481 aggcgcgaat ggaaagcccg ccccgctcgc tggtgccaag ctccagcatc ccgttatggt
    63541 tatccacgat acgctgcaca attgccagcc ctaatcccgt gccgctaatg gtgcgcgcac
    63601 tgtcgccgcg gacaaacggc tggaacaggt gcttacgttg ttccggcgca attcccggac
    63661 cgtcatcttc cacctggaac caggcgcgat tcggctccgt tccgctgctg actttgatcc
    63721 agccattgcc ataacgggcg gcgttgacca ccatattcgc caccgcgcgt ttgatcgaca
    63781 gcgggtgcat tttcacttca atgctgccgg ggtaaagcgc ggtttcaatt tcccgctcat
    63841 agccactttc ggcagcaatc acctcaccga gcactgcatt aagatccgcc atttccatcg
    63901 gcatctcctg cccggtgcgc aggtagtcga taaactgctc aatgatggcg ttgcactctt
    63961 cgatatcttt attgatcgat tctgccagat agccatcctg ctcgctcatc atctcagtcg
    64021 ccaggcgaat acgcgtcagc ggcgtgcgca agtcgtgact tacccccgcc atcagcagcg
    64081 tgcggtcatc cgccagttgc ttaacaccag ccgccatatg gttaaaggca cgggtaacgg
    64141 aacgcacctc cgaagcgcca tattcacgca gcggcggcgg aataatccct ttaccaacct
    64201 gcaaggctgc gtgttcgaga tcgaccaacg gtcggttctg gatacgaata aacagccacg
    64261 ccccgcctat cgccaatagc ataatcgcca gcgtatagcg gaacagcgga gagaaatcgc
    64321 cctgatgaat ttcggtcagc ggcacgcgta cccagatatt gggcgacagc caggttttca
    64381 gccagacgac aggcgaactt ttgttgacct caacgcgcac ttccgtcggg ccgcccagtt
    64441 gctgtgccat ctgatggctt aagaattcat agtgttgcgc ccaacgcaga cctgcctctt
    64501 cggcagcctc gttggagtag agagagatcc ccagctcacg gtagatctcc cgacggaaag
    64561 cgggaggcac aaccaactgc gtgccgtcct ccagttgcag tttgtcggtc atcaacatac
    64621 gcacttcgta cgcgaggact ttattaaact gctggaggct cggcaaaatc gcgaagttca
    64681 gcaccaccag ataagtcgtc accaggctgg cgaacagcaa ggtgacgatg agcaataacg
    64741 tacgggcaaa tgaacttcgt ggcgagaagc gcaatcgcct catgctttag agccgtccgg
    64801 tacaaagacg tagcccaggc cccaaacggt ctgaatgtaa cgcggatgcg ctggatcttc
    64861 ttctaccatg cggcgcagac gcgaaatctg cacgtcgatg gagcgttcca ttgcggaata
    64921 ttcacgacca cgggcaaggt tcatcagctt atcgcgggag agcggctcac gcggatggct
    64981 gaccagtgcc ttcagtaccg caaactcacc gctggtgagc ggcatcggct catcttcgcg
    65041 gaacatttcg cgcgtaccga ggttaagttt gaacttaccg aaagcaatta ccgcctcttc
    65101 ctgtgacggt gcgcctggca gttcgttcgc ctgacgacgc agcaccgcac gaatacgggc
    65161 cagcagttca cgcgggttaa acggttttgg aatgtagtcg tcagcgccaa tctccaggcc
    65221 tacgatacgg tccacttctt cccctttcgc cgtcaccata atgatcggca tcgggttgct
    65281 ctgactacga agacgtcggc aaatcgacaa gccatcttca ccaggtaaca ttaaatccag
    65341 taccataaga tggaaagatt cacgagtcag caggcgatcc atctgttctg cattagcgac
    65401 gcttcgaacc tggaagcctt gttcggtgag ataacgttcc agcagcgcac gcaggcgcat
    65461 gtcgtcatcg accaccagaa tcttgtagtt ctcttgcatt gtttgtactc ccaaaggttc
    65521 gcaacaattt gtaagcgtgt attcttaaaa aagctcacgt tcgtcaccag ctaaatctgg
    65581 tatgaatttc agcctaaatt gttacaaagc atattaaaca gcagcttaag tatacaattt
    65641 attcggcgaa acattattga ttctgttgat atgatcacgt tatacccaat gtgcgcatta
    65701 tcaaacagac aaagggaatc aacgagatga aaacgcccct ggttacccgg gaagggtatg
    65761 aaaaactcaa acaagagctt aattatctct ggcgtgaaga acgcccggag gtcacaaaaa
    65821 aggtgacctg ggccgcaagt ctgggcgacc gcagcgaaaa tgctgactat cagtataata
    65881 aaaagcgtct gcgtgaaatc gaccgtcgcg tgcgctatct cactaaatgc ctggaaaatc
    65941 tcaaaatcgt cgattactcc cctcagcagg aaggcaaagt cttttttggc gcgtgggtgg
    66001 agattgaaaa cgacgatggc gtgactcacc gtttccgtat tgtcggctac gatgaaattt
    66061 ttggccgtaa agattacatc tctatcgatt ccccgatggc ccgcgcattg ctgaaaaaag
    66121 aagtcggcga tctggcggtg gtgaataccc ctgccgggga agcgaactgg tatgttaatg
    66181 ctatcgagta cgtgaaaccg taaggaagag tcttaacctc ctgccgatgg ctggcatttt
    66241 tgccagccag tccgtataac tatcccctga tttttgatcc gaaaagatga actcaaacca
    66301 tgatgaatga ttcgttctgc cgcattattg cgggtgaaat tcaggcgcgc ccggaacagg
    66361 ttgacgctgc cgttcgcctg cttgacgaag ggaataccgt gccgtttatc gcacgttatc
    66421 gtaaggaaat caccggcggt ctggatgaca cgcagctgcg taatctggaa acgcgtctga
    66481 gctatctgcg cgagctggaa gagagacgtc aggcgatcct caaatccatt tccgagcaag
    66541 gcaaactcac cgatgatctg gcgaaggcca tcaacgccac cctaagcaaa accgaactcg
    66601 aagacctcta cctgccctac aaacctaaac gccgcacccg cgggcaaatc gccattgaag
    66661 cagggcttga gccgttggct gacctgctgt ggagcgatcc gtcacacacg ccagaagtcg
    66721 ccgctgcaca atatgttgat gccgataaag gcgtggcaga taccaaagcc gcgctggatg
    66781 gcgcgcgcta tatcctgatg gaacggtttg ccgaagatgc cgcgctgctg gcgaaagtgc
    66841 gtgattatct gtggaagaac gcgcatttgg tttctacggt ggtgagcggt aaagaagagg
    66901 aaggggcgaa attccgcgac tatttcgatc atcacgaacc gttgtccacg gtgccttctc
    66961 accgcgcgct ggcgatgttc cgtgggcgta acgaaggcgt actccagctt tcgctgaatg
    67021 ccgatccgca gttcgatgag ccgcccaaag agagctattg cgagcaaatc atcatggatc
    67081 accttggcct gcgcctgaac aatgccccgg cggatagctg gcgcaaaagc gtagtgagct
    67141 ggacctggcg catcaaggtg ctgatgcatc tggaaaccga actgatgggt accgtgcgcg
    67201 aacgcgcgga agatgaagca atcaacgtct ttgcccgtaa cctgcacgat ctgctgatgg
    67261 cggcccctgc cggactgcgt gcaacgatgg gcctcgatcc gggtctgcgt accggggtaa
    67321 aagtggcggt ggtcgatgcc actggcaaac tggtggcgac cgataccatt tacccgcaca
    67381 ccggacaagc cgcaaaagca gcgatgaccg ttgctgcctt gtgtgaaaaa cataacgttg
    67441 aactggtggc gatcggcaac ggtacagctt cccgcgaaac tgaacgtttc tatctcgacg
    67501 tgcagaagca gttcccgaaa gtgaccgcac agaaagtgat cgtcagcgaa gctggcgcgt
    67561 cggtttactc agcttccgag ctggcagcgc aggagttccc ggatctcgac gtttcgctgc
    67621 gtggtgcggt atctatcgcc cgccgtttgc aggatccgct ggcggagctg gtgaaaatcg
    67681 atccgaaatc tatcggcgta ggtcagtatc agcatgacgt cagccagacg caactggccc
    67741 gcaaactgga tgcagtagta gaagactgcg taaacgccgt tggcgtcgat ctcaacaccg
    67801 cttctgttcc gctattaacc cgcgtggcgg gcctgacgcg catgatggcg caaaacatcg
    67861 tggcctggcg cgatgagaac ggccagttcc agaaccgtca gcaactgctg aaagtcagcc
    67921 gtctggggcc gaaagccttc gagcagtgcg cgggcttcct gcgcattaac cacggtgata
    67981 acccgctgga cgcctctacc gttcatccgg aagcctatcc ggtggtggaa cgcattctgg
    68041 cagcaacaca gcaggcactg aaagatctga tgggtaacag cagcgaactg cgtaacctga
    68101 aagcgtctga ctttactgat gaaaaattcg gtgtgccgac agtaactgac atcatcaaag
    68161 agctggaaaa accgggtcgc gatccgcgtc cggaatttaa aaccgctcag tttgccgatg
    68221 gcgtcgagac aatgaacgac ctgcaaccgg gtatgatcct cgaaggcgca gtgaccaacg
    68281 tcaccaactt tggcgcgttt gtcgatattg gcgtgcatca ggacggcctg gttcacatct
    68341 cttcattgtc gaacaagttt gtggaagatc cgcataccgt ggtgaaagcg ggcgacattg
    68401 tgaaggtgaa agtgctggaa gtggatcttc agcgtaaacg tatcgccctg actatgcgtc
    68461 tggacgagca gcctggcgaa accaactctc gtcgcggcgg cggtaatgaa cgcccgcaaa
    68521 acaaccgccc ggcagccaaa ccacgcggtc gtgaagcgca gcctgccggt aatagcgcga
    68581 tgatggatgc gctggcggca gcgatgggta aaaaacgtta aacgcccgga ctggccccta
    68641 aacctgcgcg ataaggcgtt cacgccgcat ctggcaacga acgctttgcc tgacataaaa
    68701 gtgccggaga atatctccgg cacttttatt ccacagccaa actcacaata tactccgtta
    68761 atatttatca cttcattaac aactgaaacc ttaattaaac attagccagt ccgggtaact
    68821 cactattcga attatatttt cgctgcgata taaccttgag ccacatcaac attgagtcag
    68881 attattattc aaaccaacat tcgcacacat tttaagtatt gctgatagaa accattctca
    68941 ttatcattgt gttgttgatt atttaatctc tcctttgttg gcaaatcatc tggtctcatg
    69001 tcgcggtcaa acgccccatg aggtagttat ccagttaatg agaaacaagt aggcacctat
    69061 gcaatacact ccagatactg cgtggaaaat cactggcttt tcccgtgaaa tcagcccggc
    69121 atatcgccaa aaactgcttt ctctaggcat gttacctggc tcctcttttg atgtggtgcg
    69181 cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc tggtattacg
    69241 caaaaaagat ctggccttat tagaagtgga agcggtttcc tgttaatacg gtgataacaa
    69301 caatgaaaaa attaaccatt ggcttaattg gtaatccaaa ttctggcaag acaaccttat
    69361 ttaaccagct cactggcgca cgtcagcgtg taggtaactg ggctggcgtt accgtcgaac
    69421 gtaaagaagg gcaattctcc accaccgatc atcaggtcac gctggtggac ctgcccggca
    69481 cctattctct gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc gcctgtcact
    69541 acattttgag tggcgacgcc gacctgctga ttaacgtggt ggatgcgtct aaccttgagc
    69601 gtaacctgta cctgacgctg caactgctgg aactcggcat tccctgcatc gtggcgctga
    69661 acatgctcga cattgccgag aagcaaaata ttcgtattga aattgatgct ctatcggcac
    69721 gtcttggctg cccggtgatc ccgctggttt caacccgtgg tcgcggtatt gaagcgctca
    69781 agctggcgat tgatcgctat aaagctaacg agaatgtgga actggtgcat tacgcacagc
    69841 cgctactcaa cgaagcagat tcactggcaa aagtgatgcc ttccgacatc ccgctgaaac
    69901 aacgtcgctg gctgggcctg caaatgctgg aaggcgatat ctacagccgc gcctacgccg
    69961 gtgaagcgtc gcagcatctg gatgccaccc tcgcccgtct gcgtaatgaa atggatgatc
    70021 cggcgctgca cattgccgat gcgcgttacc agtgcattgc tgccatctgt gatgtggtaa
    70081 gcaacaccct gacggcagaa cccagccgtt tcaccactgc ggtagataaa atcgtgctca
    70141 accgtttcct cggtctgccg attttcctct ttgtgatgta cctgatgttc ctgctggcta
    70201 tcaacatcgg cggggcgtta cagccgctgt ttaacgtcgg ctccgtggcg ctatttgtgc
    70261 atggtattca atggattggc tacacgctcc acttcccgga ctggctgact atcttcctcg
    70321 cccagggcct gggtggcggt attaacaccg tgctgccact ggtgccgcag atcggcatga
    70381 tgtacctgtt cctctccttc cttgaggact ccggctatat ggcgcgtgca gcgtttgtga
    70441 tggaccgcct gatgcaggcg ctgggcttgc cggggaaatc ctttgtgccg ctgatcgtcg
    70501 gtttcggttg taacgtaccg tcggtaatgg gcgcacgtac gcttgatgca ccgcgtgaac
    70561 gtctgatgac catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg gctatcttcg
    70621 cagtattcgc ggctgccttc ttcgggcaga acggtgccct ggcggttttc tcgctgtata
    70681 tgctcggtat cgtgatggcg gtgctgactg gcctgatgct caagtacacc atcatgcgcg
    70741 gtgaagcgac gccgtttgtc atggagctgc cggtctacca tgtaccacac gttaaaagcc
    70801 tgattatcca gacctggcag cgtctgaagg gcttcgttct gcgtgctggt aaagtgatta
    70861 tcatcgtcag cattttcctg agcgctttca acagcttctc gttgagcggg aaaatcgtcg
    70921 ataacatcaa cgactcggcg ctggcttccg tcagccgggt gataacccct gtcttcaagc
    70981 cgattggcgt acatgaagat aactggcagg caacggttgg cctgtttacg ggcgcaatgg
    71041 caaaagaagt ggtggtgggt acgctcaaca ccctctacac cgcagaaaat attcaggacg
    71101 aagagttcaa tccggcggaa ttcaacctcg gtgaagagct gttcagtgcg gtagatgaaa
    71161 cctggcagag cctgaaagac accttcagcc ttagcgtact gatgaacccc attgaagcta
    71221 gcaaaggcaa cggtgaaatg ggtaccgggg cgatgggcgt gatggatcag aaattcggta
    71281 gcgcagctgc cgcatacagc tacctgattt tcgtcctgct gtatgtacca tgtatctcgg
    71341 tgatgggagc catcgcccgt gaatcaagcc gtggctggat gggcttctcc atcctgtggg
    71401 ggctgaatat cgcttactca ctggcaacat tgttctatca ggtcgccagc tacagtcagc
    71461 acccaactta cagcctggtg tgcattctgg cggtaatcct gtttaacatc gtagctatcg
    71521 gtctgctgcg ccgcgcgcgt agccgggtgg atatcgaact gctggcaacc cgcaagtcgg
    71581 taagcagttg ctgcgcagcc agcaccaccg gtgattgcca ttaatggctt cacttattca
    71641 ggtacgtgat ttgctggcgt tacggggccg tatggaagcg acccagataa gccagacatt
    71701 gaacactccg cagccaatga ttaacgccat gctgcaacag ctggaaagca tgggcaaagc
    71761 cgtgcggatt caggaagaac ctgacggctg cctctctggc agttgtaaaa gctgcccgga
    71821 aggcaaagcc tgtctgcgcg agtggtgggc gctgcgttaa ccatactcca tcgcctgttt
    71881 tgaaaagccc ggtatgcgtt tgcatccggg cttttttgcg tgcggctttc cataaaaatg
    71941 caattcttgc agcgcaacgt aagttcctct gaaagcgtct cgcagggatg aaaactcgct
    72001 aatgcacaga tgtggagtgg cgcgtagagt cgcggcattc aaacaacagg tgaaggaacg
    72061 ccatgagtaa aaagcagagt tccaccccac atgatgcgct gttcaaactc tttttacgcc
    72121 aaccggagac ggcacgcgat tttcttgcgt ttcatttacc ggcacccatt cacgcgctct
    72181 gtgatatgaa aaccctcaag ctggagtcga ggagctttat tgatgacgat ctgcgtgaaa
    72241 gctattccga tgtgctgtgg tcggtgaaaa cggaacaagg accaggatac atctattgtc
    72301 tgattgaaca tcaaagcacc tcaaacaaac tgatcgcatt tcgcatgatg cgttacgcta
    72361 ttgccgcaat gcaaaatcac cttgatgctg gatacaaaac gttgccgatg gtggtgccat
    72421 tgttgtttta ccacggtatt gaaagcccct atccctattc gctgtgttgg ctggattgtt
    72481 tcgccgatcc caaactggca aggcagcttt atgcctccgc atttccgctg attgatgtca
    72541 ccgtcatgcc tgatgatgaa atcatgcagc accgacgtat ggcgctgctg gagttaattc
    72601 aaaaacatat tcgtcaacgc gatctgatgg ggctggtaga gcaaatggcc tgcttattaa
    72661 gtagtggata cgctaatgac agacaaatca aagggctgtt taattacata ctgcaaactg
    72721 gcgacgctgt acgttttaac gattttatcg acggcgttgc cgaacgttca ccgaaacaca
    72781 aggagagttt aatgactatt gcggaaagat tgcggcagga gggggaacaa tccaaagccc
    72841 tgcatatagc caaaataatg cttgaatccg gagtccctct tgcagacatc atgcgattta
    72901 ccgggctgtc agaagaagag ttggctgcgg
//
LOCUS       DAJTUM010000005        72097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTUM010000005 DAJTUM010000000
VERSION     DAJTUM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1370..2956)
                     /locus_tag="OO471_000387"
     CDS             complement(1370..2956)
                     /locus_tag="OO471_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6241781.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3311..5482)
                     /gene="glcB"
                     /locus_tag="OO471_000388"
     CDS             complement(3311..5482)
                     /gene="glcB"
                     /locus_tag="OO471_000388"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6241782.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5504..5908)
                     /locus_tag="OO471_000389"
     CDS             complement(5504..5908)
                     /locus_tag="OO471_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6241783.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5913..7132)
                     /gene="glcF"
                     /locus_tag="OO471_000390"
                     /pseudo
     CDS             complement(5913..7132)
                     /gene="glcF"
                     /locus_tag="OO471_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7143..8195)
                     /gene="glcE"
                     /locus_tag="OO471_000391"
     CDS             complement(7143..8195)
                     /gene="glcE"
                     /locus_tag="OO471_000391"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6241784.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8195..9694)
                     /gene="glcD"
                     /locus_tag="OO471_000392"
                     /pseudo
     CDS             complement(8195..9694)
                     /gene="glcD"
                     /locus_tag="OO471_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9945..10715
                     /gene="glcC"
                     /locus_tag="OO471_000393"
     CDS             9945..10715
                     /gene="glcC"
                     /locus_tag="OO471_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6241785.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10839..11980)
                     /locus_tag="OO471_000394"
                     /pseudo
     CDS             complement(10839..11980)
                     /locus_tag="OO471_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12342..14072
                     /locus_tag="OO471_000395"
     CDS             12342..14072
                     /locus_tag="OO471_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6241786.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14069..14983
                     /locus_tag="OO471_000396"
     CDS             14069..14983
                     /locus_tag="OO471_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6241787.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15016..15264
                     /locus_tag="OO471_000397"
     CDS             15016..15264
                     /locus_tag="OO471_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6241788.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15264..16435
                     /locus_tag="OO471_000398"
                     /pseudo
     CDS             15264..16435
                     /locus_tag="OO471_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16471..17550)
                     /gene="lptG"
                     /locus_tag="OO471_000399"
     CDS             complement(16471..17550)
                     /gene="lptG"
                     /locus_tag="OO471_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6241789.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17547..18617)
                     /gene="lptF"
                     /locus_tag="OO471_000400"
     CDS             complement(17547..18617)
                     /gene="lptF"
                     /locus_tag="OO471_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6241790.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18648..19208)
                     /locus_tag="OO471_000401"
     CDS             complement(18648..19208)
                     /locus_tag="OO471_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6241791.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19220..20044)
                     /locus_tag="OO471_000402"
     CDS             complement(19220..20044)
                     /locus_tag="OO471_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241792.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20044..21393)
                     /locus_tag="OO471_000403"
     CDS             complement(20044..21393)
                     /locus_tag="OO471_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6241793.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21439..22197)
                     /locus_tag="OO471_000404"
     CDS             complement(21439..22197)
                     /locus_tag="OO471_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241794.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22229..22942)
                     /locus_tag="OO471_000405"
     CDS             complement(22229..22942)
                     /locus_tag="OO471_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6241795.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23116..23808
                     /locus_tag="OO471_000406"
     CDS             23116..23808
                     /locus_tag="OO471_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6241796.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23857..25356)
                     /gene="pitB"
                     /locus_tag="OO471_000407"
     CDS             complement(23857..25356)
                     /gene="pitB"
                     /locus_tag="OO471_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6241797.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25649..27508)
                     /gene="gss"
                     /locus_tag="OO471_000408"
     CDS             complement(25649..27508)
                     /gene="gss"
                     /locus_tag="OO471_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6241798.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27713..28579
                     /gene="yghU"
                     /locus_tag="OO471_000409"
     CDS             27713..28579
                     /gene="yghU"
                     /locus_tag="OO471_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6241799.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28702..28950)
                     /gene="hybG"
                     /locus_tag="OO471_000410"
     CDS             complement(28702..28950)
                     /gene="hybG"
                     /locus_tag="OO471_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6241800.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29084..29425)
                     /gene="hypA"
                     /locus_tag="OO471_000411"
     CDS             complement(29084..29425)
                     /gene="hypA"
                     /locus_tag="OO471_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6241801.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29418..29906)
                     /gene="hybE"
                     /locus_tag="OO471_000412"
     CDS             complement(29418..29906)
                     /gene="hybE"
                     /locus_tag="OO471_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6241802.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29899..30393)
                     /locus_tag="OO471_000413"
     CDS             complement(29899..30393)
                     /locus_tag="OO471_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6241803.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30393..32096)
                     /gene="hybC"
                     /locus_tag="OO471_000414"
     CDS             complement(30393..32096)
                     /gene="hybC"
                     /locus_tag="OO471_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6241804.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32093..33271)
                     /gene="hybB"
                     /locus_tag="OO471_000415"
     CDS             complement(32093..33271)
                     /gene="hybB"
                     /locus_tag="OO471_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6241805.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33261..34247)
                     /gene="hybA"
                     /locus_tag="OO471_000416"
     CDS             complement(33261..34247)
                     /gene="hybA"
                     /locus_tag="OO471_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6241806.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34250..35368)
                     /gene="hybO"
                     /locus_tag="OO471_000417"
     CDS             complement(34250..35368)
                     /gene="hybO"
                     /locus_tag="OO471_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6241807.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35557..35844)
                     /locus_tag="OO471_000418"
     CDS             complement(35557..35844)
                     /locus_tag="OO471_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6241808.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35963..36850)
                     /locus_tag="OO471_000419"
     CDS             complement(35963..36850)
                     /locus_tag="OO471_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6241809.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37007..38047
                     /gene="gpr"
                     /locus_tag="OO471_000420"
     CDS             37007..38047
                     /gene="gpr"
                     /locus_tag="OO471_000420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6241810.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38087..38581)
                     /locus_tag="OO471_000421"
     CDS             complement(38087..38581)
                     /locus_tag="OO471_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6241811.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38772..39656
                     /gene="yghA"
                     /locus_tag="OO471_000422"
     CDS             38772..39656
                     /gene="yghA"
                     /locus_tag="OO471_000422"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6241812.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39928..40353)
                     /gene="exbD"
                     /locus_tag="OO471_000423"
     CDS             complement(39928..40353)
                     /gene="exbD"
                     /locus_tag="OO471_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6241813.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40360..41094)
                     /gene="exbB"
                     /locus_tag="OO471_000424"
     CDS             complement(40360..41094)
                     /gene="exbB"
                     /locus_tag="OO471_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6241814.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41346..42533
                     /gene="metC"
                     /locus_tag="OO471_000425"
     CDS             41346..42533
                     /gene="metC"
                     /locus_tag="OO471_000425"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6241815.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42673..43332
                     /locus_tag="OO471_000426"
     CDS             42673..43332
                     /locus_tag="OO471_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6241816.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43372..44271)
                     /gene="yqhC"
                     /locus_tag="OO471_000427"
     CDS             complement(43372..44271)
                     /gene="yqhC"
                     /locus_tag="OO471_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6241817.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44465..45628
                     /gene="yqhD"
                     /locus_tag="OO471_000428"
     CDS             44465..45628
                     /gene="yqhD"
                     /locus_tag="OO471_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6241818.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45733..46560
                     /gene="dkgA"
                     /locus_tag="OO471_000429"
     CDS             45733..46560
                     /gene="dkgA"
                     /locus_tag="OO471_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6241819.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46760..47686
                     /locus_tag="OO471_000430"
     CDS             46760..47686
                     /locus_tag="OO471_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6241820.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47737..47994
                     /gene="yqhH"
                     /locus_tag="OO471_000431"
     CDS             47737..47994
                     /gene="yqhH"
                     /locus_tag="OO471_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6241821.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48037..50256)
                     /locus_tag="OO471_000432"
     CDS             complement(48037..50256)
                     /locus_tag="OO471_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6241822.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50367..51779)
                     /gene="ftsP"
                     /locus_tag="OO471_000433"
     CDS             complement(50367..51779)
                     /gene="ftsP"
                     /locus_tag="OO471_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6241823.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51854..52591)
                     /gene="plsC"
                     /locus_tag="OO471_000434"
                     /gene_synonym="parF"
     CDS             complement(51854..52591)
                     /gene="plsC"
                     /locus_tag="OO471_000434"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6241824.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52825..55083)
                     /gene="parC"
                     /locus_tag="OO471_000435"
     CDS             complement(52825..55083)
                     /gene="parC"
                     /locus_tag="OO471_000435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6241825.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55629..56111)
                     /locus_tag="OO471_000436"
     CDS             complement(55629..56111)
                     /locus_tag="OO471_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6241826.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56164..56556)
                     /gene="ygiW"
                     /locus_tag="OO471_000437"
     CDS             complement(56164..56556)
                     /gene="ygiW"
                     /locus_tag="OO471_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6241827.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56708..57367
                     /gene="qseB"
                     /locus_tag="OO471_000438"
     CDS             56708..57367
                     /gene="qseB"
                     /locus_tag="OO471_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6241828.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57364..58638
                     /gene="qseC"
                     /locus_tag="OO471_000439"
     CDS             57364..58638
                     /gene="qseC"
                     /locus_tag="OO471_000439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6241829.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58684..59016)
                     /locus_tag="OO471_000440"
     CDS             complement(58684..59016)
                     /locus_tag="OO471_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6241830.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59335..59916
                     /gene="mdaB"
                     /locus_tag="OO471_000441"
     CDS             59335..59916
                     /gene="mdaB"
                     /locus_tag="OO471_000441"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6241831.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59947..60261
                     /locus_tag="OO471_000442"
     CDS             59947..60261
                     /locus_tag="OO471_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6241832.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60309..62201)
                     /gene="parE"
                     /locus_tag="OO471_000443"
     CDS             complement(60309..62201)
                     /gene="parE"
                     /locus_tag="OO471_000443"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6241833.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62230..62811)
                     /gene="yqiA"
                     /locus_tag="OO471_000444"
     CDS             complement(62230..62811)
                     /gene="yqiA"
                     /locus_tag="OO471_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6241834.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62811..63638)
                     /gene="cpdA"
                     /locus_tag="OO471_000445"
     CDS             complement(62811..63638)
                     /gene="cpdA"
                     /locus_tag="OO471_000445"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6241835.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63663..64085)
                     /locus_tag="OO471_000446"
     CDS             complement(63663..64085)
                     /locus_tag="OO471_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6241836.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64086..64715)
                     /gene="nudF"
                     /locus_tag="OO471_000447"
     CDS             complement(64086..64715)
                     /gene="nudF"
                     /locus_tag="OO471_000447"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6241837.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64920..66401
                     /gene="tolC"
                     /locus_tag="OO471_000448"
     CDS             64920..66401
                     /gene="tolC"
                     /locus_tag="OO471_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6241838.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66549..67220
                     /locus_tag="OO471_000449"
     CDS             66549..67220
                     /locus_tag="OO471_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6241839.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67226..68386
                     /locus_tag="OO471_000450"
     CDS             67226..68386
                     /locus_tag="OO471_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6241840.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68424..69212)
                     /gene="ygiD"
                     /locus_tag="OO471_000451"
     CDS             complement(68424..69212)
                     /gene="ygiD"
                     /locus_tag="OO471_000451"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6241841.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69355..70128
                     /gene="zupT"
                     /locus_tag="OO471_000452"
     CDS             69355..70128
                     /gene="zupT"
                     /locus_tag="OO471_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6241842.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70186..70356)
                     /locus_tag="OO471_000453"
     CDS             complement(70186..70356)
                     /locus_tag="OO471_000453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241843.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70617..71270)
                     /gene="ribB"
                     /locus_tag="OO471_000454"
     CDS             complement(70617..71270)
                     /gene="ribB"
                     /locus_tag="OO471_000454"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6241844.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71374..71522)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71644..71934
                     /gene="ubiK"
                     /locus_tag="OO471_000455"
     CDS             71644..71934
                     /gene="ubiK"
                     /locus_tag="OO471_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6241845.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18039 a        19162 c        17738 g        17158 t
ORIGIN      
        1 tacgtaccgt ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt
       61 acagggctta ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca
      121 taagaaatca aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt
      181 cattaatact gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga
      241 gatccatcgg tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg
      301 tatcagattc ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat
      361 ttacattatt tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt
      421 aatctcttac tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctattttta
      481 gcaggaaata ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataagttaa
      541 tgattttaat gattttaatg attttaatga ttttaatgat tttaatgatt ttaatgattt
      601 taatgatttt aatgattttt tcatttattc acacctaata aaagtattca aattcagatg
      661 atttttctgc tcaacagaca caaacaggca ataatactct ccaaagcgaa caatatttgg
      721 cattacgatt taatgcttca taatcaccaa attatttaac cataaatttt cttatttcta
      781 acaaaatgat tggtgtgttg ttggagttaa aatacataca taatattaac atctgccaac
      841 atttcacttt ccgcaagacg gatgatttcg aatcctcatc cacgttaccc tatcccaaat
      901 atctttatta ataggcagta acgaacactg ttatagcaag attaacttat taacctaacg
      961 gcatctttta tgtaacaagt ggagataaat tgcttaaaat tgatcctcga caaatcttaa
     1021 aagttctcat tattctgcaa aatcagcttt gcgaataatc ttatgttttt gggagttttg
     1081 ggaattttga tctactcatt gttctagtga aaaatataaa taagagaaaa aacgccattc
     1141 gtcatgaaag caacagatga agcaatgtaa taacatacta aaattgatct catgaaagtg
     1201 ggtactttat aaccagcaca gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa
     1261 aacggagact acgtataaag aagccaatcg taaaaacggt tccctggcga gataaacgac
     1321 agacaaatgg aaagccgggt gatatttacc ttcatccggc tttattgatt tacgagacta
     1381 acatcccggt aaacacatac gcctgcagca gggtgataac gctggcaaaa atcagactgt
     1441 gcttcacggt gtagcggaac agttcagatt ctcggcccac catgcccgtc gcggcgcagg
     1501 ccacggcgat agattgcggg gagatcatct tgccagttac gccgccgctg gtatttgctg
     1561 ccaccagcag agtgtcagag acgttgattt gctgcgccgt ggtcgattgc agtgaaccaa
     1621 acagggcgtt tgaagaagtg tccgacccgg taaggaatac gcccagccag ccgagaaacg
     1681 gtgagaaaaa cgggaacatc acgcctgtac ctgccagtac cagcgccagc gtggtggaca
     1741 tgccggaata gttggtgacg aaggcgaatg ccagcaccat gccaatcgac aatatcggcc
     1801 acttcagact aattagcatt tcggcaaaga cgccaatccc tttcttgatg cccacgccga
     1861 ggatgaagat agagataatc gccgcaataa aaatagcggt gccgccagcc gagagagggt
     1921 cgaatttaaa caccgcatcc attggcgttg gttgggcgac aatgggtgcc gctttcaaca
     1981 cttgttgatg caaatgaggg atctggaaat taatcaccag tgaataaaac gcgccgcccg
     2041 gagcaaataa cgctttaaac ggcttcatgg tccagatggt gaccagcacc gttaagatta
     2101 aaaacggtga ccacgcacga atgatttgcc ccagactata ttctgaaggc acgggaccgc
     2161 cagaagatgg cttatttacc accatcgcac ctgcggattg ccccatgctg attgccgttt
     2221 cggtattttt cggccgccag actttaagga ataaagcgag tgagacgata ctcaccagcg
     2281 ccgaagtaat atccggcagt tccggaccaa tatagttaga ggtaaagaac tgagtgacag
     2341 cgaagcttcc cccagcaacc agcgccgctg gccacgtctc tttcacccct ttccagccgt
     2401 ccatcattgc taccagccag aacggcacaa gaaccgacag gaacggtaac tgacgtcccg
     2461 ccattgcgcc aatgtggaac ggatcgattc ccgttacctg accggcgacc agaatcggca
     2521 cgcccaacgc accaaacgcc accgtcgcac caaagccagc agctccttcc agcaacgcac
     2581 caaaggagaa accaatcagt aacacctgca aacgctgatc gtcggtgatg gagataaccg
     2641 agctgcggat aatatcgaac tgcccgctgg caacggttaa tttatacagg aacaccgccg
     2701 cgacaataat ccacgcaatt ggccataatc cataaataaa gccatagccc gcagcagcaa
     2761 atgccatatc aatcggcatt ttaaaggcga atatcgcaat caggatagat aatataaggg
     2821 ttattgctcc agcaacatgt cctttcagac gtaataccgc gagtgcaacg aagaagaata
     2881 ttatcgggat cagggcgacc agagcggata gccccagtcc tcccatcggc atatacattt
     2941 gggtccaggt aaccatattg ttcagtctct tattatcttt atatgcttga tatacttaag
     3001 gttgtaataa gcaaaagagg actgaactgt aaaatatagg cgttatactt tacagcaaca
     3061 gtacgccgct aacgcaattg ctacctctgg cataacaagt atatcgggta agggtttctg
     3121 ttccgcacac gcaggcgcag agtatcgtta agatgtccat attgttgttt taggcccgct
     3181 agtaatgcgc tacgggtatt taatattgtt aaaccctgat aatcgctccg gttatttccg
     3241 ggataaatgt actaccgcag ttactatcat agccccgaca ataaaacttg ccggggcttt
     3301 tttgacgcta ttaatgactt tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg
     3361 tataaccgtt tggctgtttc acgccgagga agattaaatc gctggcagct ttaaaagcac
     3421 acgagttagc gaaattcccc gccatcggac gataagccgg atcgccagca ttttgctgat
     3481 caaccacttt cgccatattc tccagcgacg cctgcacctg ttctttggtc agaataccgt
     3541 gacgtaacca gttggcgata tgctggctgg agatacgcag cgttgcgcgg tcttccatca
     3601 gcgccacgtt gtgaatatcc ggcacttttg aacaaccaat cccctgctcc acccaacgca
     3661 ccacgtaccc cagaatcccc tgcacgttgt tatccagctc ttgttggatc tcttgcgccg
     3721 accagttagc gttttcagcc accggaatag tcagcagatc gtccagcagc ggttcaaatt
     3781 cagcactgaa ctcggtctgg gcaatgttgg cttgcacgct ctgtacgttg gtatggtggt
     3841 agtgcagcgc atggagcgta gcagcggttg gtgacggaac ccaggctgtg tttgccccgg
     3901 cacgcagttg gtcgcccttc tggctgtaca tgtctgccat caggtccggc attgcccaca
     3961 tgcctttacc gatttgcgct ttaccgcgca gcccacagaa cagaccggaa agcacgttat
     4021 tacgttcgta ggctttgatc cacggcgtcg atttcatctg gtttttacgc agcatcgggc
     4081 cagcttccat caccgaatgc atttcatcgc cggtacggtc gaggaaaccg gtattgatga
     4141 acgccacgcg gttgcgcgcc tgagcgatac agctacgcaa gttcagcgag gtccggcgtt
     4201 cttcatccat aatgcccatt ttcagggtat tcggtgccat accgagcatt gtctcaatgc
     4261 gggtaaacag tttgttggcg aacgccactt cctgcggacc gtgcattttc ggtttcacaa
     4321 tatagacgct gccagtgcgc gagtttttct gcacttttaa atcatagagg gcaatcgcgc
     4381 cagtcatgac gccatcaaga ataccttccg ggatttcatt gccttcgctg tcccaaatca
     4441 caggaatggt catcaaatga cccacgttgc ggataaacag caacgagcgt ccgtgcagag
     4501 aaatttcaga gccatcggcg gcggtgtact gacgatcgtc attcagttta cgtacgattt
     4561 gccgaccgtt tttctccatt ttctcttgca gagtcccctg catcaggccc agcagattgc
     4621 ggtacagcag gattttatct tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa
     4681 tggtactgat agcagcttcg acgataacat cgttgatgtg cgccggatcg tctttgccaa
     4741 tccgcccgtt ggcatcaatt tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc
     4801 aggtcggcgc agcggcatcg ccacggtaac cgacaaactg tgctggagta cgtaacgtgg
     4861 tttctttacc atttttcaac tggatgcgta attgtttatc gaccacctta aacgccacca
     4921 catcctgata gctgccgttt tccagcggta gtgattcatc gaggaaacgt cgaacccagg
     4981 cgataacctg ctcaccgcgc tgcggatcgt agccactgac catcgcccct tcctgcggga
     5041 tgatgtcgct gccgtataac gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca
     5101 gcgcgtagcg ggcgttcatt gccggaacca ccagctgtgg ccccgcctga ctggtgattt
     5161 cgctgtcaat gcccgtggtt tccaccgtca cgcgctccgg ttgcggcacc aggtagccca
     5221 gttcacgaag gaaagattta taggccgctt tatcttttac cggccccgga ttgctgcgat
     5281 gccactcatc aagcgccgcc tgaatgcgat cgcgttctgc cagcaactga cgattttctg
     5341 gtgccagatc gtgaacgatc tcatcaacat tgtgccagaa tgcagcagcg tccagttcca
     5401 cgcccggcaa aacttctcca tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc
     5461 cctgggttat ggtttgactc attgtttctc tcctcgtttt cgcttatttc gccaacaccg
     5521 ctgctgccac tttcgcgacc tgcgcatcct gtgctccggt taaaccagaa acgcccacgg
     5581 caccaataat ttgcccatca acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg
     5641 gcgcagtcac gaacgcggta cgtccgttgt tcaccatctc ttcatagccc ttagtttcac
     5701 gacgccccag cgcggcggta cgcgctttct cctgggagat gtaagccgca atcggcgcgc
     5761 aatcgtccat gcgacttaac gccagcagat gaccgccgtc atcggcaaca gcaatggaaa
     5821 cagaccagtt atttttctgc gcctcttcct gacctgcggc aataattgca ctcgccattt
     5881 gctggctaag aatgacttta gttttcattt ttttattcct tttcaagggc ttgttctaca
     5941 atttcaatcc agtgacgcac agaggtacga ccggcgctcg ccagatgcgt ctggcaacca
     6001 atgttggcgg tgacgatcat ttccggtttg ccgctttcca gcgcattcat tttgttatcc
     6061 cgcagctggc gtgccagatc gggatgcgtt aacgcatatg ttcccgctga accgcagcac
     6121 agatggctgt cgggaacgtc cgttaaggta aatccaagac gaagtaacac tttttccact
     6181 tcgccgttca gcttttgcgc atgttgtagg gtacacgggc agtggaaggc cagtttttta
     6241 tcgccgcgaa ttgccagttt ttccagcggt tcctcgcgca gaagttcgac taaatcgacc
     6301 gccagttcac tgacctgacg cgctttatcg gcatataacg catcgttttt cagcatctgc
     6361 ccatactctt tgacgaacgc gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct
     6421 gcttcaatcg cgggccacca ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc
     6481 tgcgcattaa gatgatagtc caccgcgcca caacagcctg cttcgttagc tgacatgacg
     6541 ctgatcccca gacgatccag cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta
     6601 ggctgggcgc agccttccag cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt
     6661 ttagctttca ccgtttcagc aggcagtttt gctctgacct gttccggtaa aaacggtcgc
     6721 agcaccagcc ctacctgcgt cagcgcacgg aagaccgccg gacgcggcac tacctggcgc
     6781 aatccttcgc gcagcattcg ctccggcagt gggcgtttca ctttctgctc gacaatatca
     6841 cgcccgatat ccagcaaatt gtgatagcgc acaccagaag gacaggtggt ttcacaattg
     6901 cggcaagtga ggcagcgatc gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc
     6961 agcacctgtt taatcagata gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc
     7021 tgataggttg ggcaggttgc ggtacaaaat ccgcagtgaa cacaggcgca caggatgctg
     7081 tcggcttcca gcgcgcgttc tgccgcatct cttcggttaa tcgggtttgc atagcctgct
     7141 cctcaaagtt ccgcgtacat gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt
     7201 ttaagctgct ggtgatagcg gaataaagga gccgatagcg gggcaaagcc accatctccg
     7261 gcactaaagc gggtcgcatg accgccagcg ttgcgggcga tgcgatggat ttgattgtca
     7321 tcggctgtcg atttcagcca gcgtaacgcc ccgccccagt cgatcagttg ctcgccgggt
     7381 aaatccatca tcggcgcatc gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag
     7441 aagaacggca gttgttgttc acgcaattgc tgccagaact gacccacaac ctcttcgcca
     7501 cccagcagtt cacgcgctgc ttttaccgat ccttcgccgc cctcaaggcg gatccacaac
     7561 gcattgtcga agtaacataa gccactaatg ggtaatggct ggagttgcca ctcggcgatt
     7621 tcactcatgg cttcttgcag gctgatttcc cgacgcaggc tcagagaggc acgcggtcgc
     7681 ggtaacactt tcattgagat ttcagtgagc acgccaagac aaccgtagct tccggccatt
     7741 aaccgtgaga gatcgtatcc ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt
     7801 tttccagtgc cggtaatgat gcgcgtgccg aggacaaaat cgcggaccga accgctccac
     7861 gggcgacgcg gccccgccag cccgcaggcg accatcccgc cccaggtggc ttcttcacca
     7921 taatgcggcg gctcgcaggg aagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc
     7981 accagcggcg ttccggcacg agcggttatc accagctcgg tcgggtcgta attaacaatg
     8041 ccgcgatgac aacgcacatc cagcgtttgc ccggtgactg ggcgacctaa aaaggctttg
     8101 ctattgctgc cctgaatcac cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc
     8161 agcagcgcct gactgtaatc acactcgcgt agcatcaaaa gcgctccagt tcaggaaaag
     8221 gtaaatgacc gtgatgcaca tgcatggcac caaattcagc acagcggtgt agcgtgggaa
     8281 tgtttttccc agggttcagc aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg
     8341 tcgtgatttc atcgctgttg aactgggcgc acatttgatt gattttttct cgcccgatgc
     8401 catgttcccc actgatgctg ccgccaactt caacgcagag ttcgaggatc ttcccgccca
     8461 gctcttccgc gcgggcaaat tcaccaggtt cgttggcatc gaaaaggatt aacgggtgca
     8521 tgttgccatc tccggcatga aagacgttgg caacacgtaa atcatattgc tgcgataaac
     8581 gggcaatgcc ttccagtacg ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt
     8641 agtaatccgg ggagatacgt cctaccgccg ggaacgcatt tttgcgaccg gcccagaaac
     8701 gtacgcgctc tgcttcgtcc tgtgccaaac ggacgtcagt cgcgcccgct ttcaacaaga
     8761 tgtagttaac ccgctcgcag tcttcctgta cgtcagactc cacgccgtcc agttcgcata
     8821 acaaaatcgc ttcggcgtcg acgggataac cggcatgaat aaaatcttcc gccgcgcgga
     8881 tcgacaggtt atccatcatc tccagcccgc cggggataat gccattggcg atgatgtcac
     8941 caaccgcaag tccggctttt tctaccgagt caaagctggc taacagaacc cgcgccacgg
     9001 gcggcttcgg cagcagtttt accgtcactt cggtggtcac gccgagcata ccttccgatc
     9061 cggtgaacag cgccagcagg tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca
     9121 gcgcctcgcc gtccagcgtt tgcacttcaa ttttcagcag gttatgtacg gtcagaccat
     9181 atttcaggca gtggacgcca ccggcatttt ccgccacatt gccgccaatt gaacaggcga
     9241 tttgtgagga aggatccggc gcgtaataga gattatgcgg cgcaacggcc taggagatcg
     9301 ccaggttaca tacgcccggc tgcacgcgcg cgcggcgacc aacggggtta atgtcgaggc
     9361 tctctttaaa gcgcgccatc accaacaaca cacctttttc cagcggcagc gcgccaccag
     9421 aaagcccggt gcctgcacca cgggtcacca ccggtacacg caggcgatgg cagacagcca
     9481 gaatcgctgt cacctgttcc atttgcttag gcagaacaac cagtaatgga cgcgtgcgat
     9541 acgcgctcaa cccgtcacac tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa
     9601 gtccagggac atgctcacgc agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag
     9661 cgccatcaag acgctcttcg tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt
     9721 gtggcagctg atttttgcgc gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt
     9781 cgtctatctc tttagctacc ggtcagacca ttttttttcc agctctgtga ccttgtcttg
     9841 gttaacttaa tgttaaattg atgtaacata atcacttgcg tgatgtgcgt gttttgcgag
     9901 ttaagaacag aaaaattggt cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc
     9961 gccctatttg cgaagtggtt gcagagagta tcgaacggtt aattatcgac ggcgtactga
    10021 aggtcggtca gccgcttccc tcggaacgtc gactgtgtga aaagctcggc ttctcacgct
    10081 ccgcactgcg tgaagggctg accgtgctgc gcgggcgcgg gattattgaa acggcgcagg
    10141 gtcgcgattc tcatgtcgca cggcttaatc gggagcagga caccagcccg ctgatccatc
    10201 tgttcagtac gcagccgcga acgctgtacg atctgctcga cgttcgcgca ttactggagg
    10261 gcgaatcggc aaggctggcg gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc
    10321 gctgttatga aaaaatgctc gccgccagtg agaaccacaa agagatttcg ctgatcgaac
    10381 atgcgcagtt ggatcacgct ttccatctcg ccatttgtca ggcttctcac aatcaggtgc
    10441 tggtgtttac gctgcaatca ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa
    10501 ataatctcta ccatcgacca cagcaaaaaa agcagatcga tcgccagcat gcgcggatct
    10561 acaacgcggt gttgcagcgg ctgccgcacg tcgctcagcg cgcagcgcgc gatcatgtac
    10621 ggaccgtgaa aaagaatctc cacgatatcg agctggaagg ccaccatttg attcgctcgg
    10681 cggtgccgct ggagatgaac aaggtgggaa tgtgagcaga gttttaccct atccccaagg
    10741 cgaggggacc gcccctgcac ttaacaggat ctggtgttaa atctgttcat ctataccctc
    10801 tcccgaatga ccgcccctgc gctaaacagg atctggtatt aaatttgctt ttcatacaat
    10861 cggtaacgct tgtacggctc cgccccaatg cgttccagca tgttattcat gcctgtattg
    10921 gtttcgagga tccatgacat ctccagcgca tcgatcttcc ggcgggcaaa cggatcgcgt
    10981 aaggcttcaa tcaataacag cgcaatcacc gggccgatgc ggctgaactg atactcgtcg
    11041 cgcacgccca tcagcggtac tcgcgcagtt cgcacaccgc tgactttcaa acgccacagc
    11101 aattttgccc agccgaaggg aaagagcgat ccgttcagat cggcaatcgc ctcgttgatg
    11161 ttaggcaagc cacaataaac gcgcagggtg cagaatcaat ctcagcgatg tagatcatat
    11221 cgtccggcac cagatattta agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg
    11281 gcacaaatcc ccagttgtgc tgccagccag agttgaaaat ctcacgcagg atctgcatct
    11341 cttcggcaaa ccgctgacga ttgatgcagc gaatggtcac ctttttgcgc acctgatcca
    11401 tcagtttttt tagcgccgga gagaaagtga gatcggttcg ctgcatccac cacgccagta
    11461 aatcaatgcc tttgtgataa cccagttgtt caatatgcgc ggcataccac ggtttgccgt
    11521 gtggcatcat cgcacagggt ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat
    11581 tgatattcag gctgaaaggc ccgctgatct tacttgcacc ttgtgacttc aaccacgctt
    11641 ccgctgcgcc aaacaacgcg gcaaaatcct gcggatcatc aatggcgtca atcatgccga
    11701 aatgaccggt atcttcaccg taacgctcac ggtgcaaggt atctatttgc gcggtaatac
    11761 gcccaactat ctgccccgct ttttttgcca cccacgcctg ccagatgata tggtccgtcc
    11821 ccggattttt cgcagacaaa tgctcgttgc gttcaatgaa taaagggggt atccagtttg
    11881 gatcgtcggg ataaagtgat gacggaaaag cgataaatgc cttaaggtca tttttattaa
    11941 ttactttttc aattttaatc atcagcagat cgcattccat atctctgtgg ataacaaata
    12001 attaatggtg aaaaacaggt tgcaacagca acgagcggca tcttaataat caactcaatg
    12061 aatacaaacg acgaataaat agtattaatt aatgattatg ataaggtcag aggctacaca
    12121 gaaacgcctc aatgcctttt gtcaatttaa ggcgaccgga caaggcttct tcttaatata
    12181 ttcataaatt aaatatcatt cataataaaa acaaaaaact taaactcatt atttaaatca
    12241 atgttcattt tcacaccgat gaaaatacta tctcatttat cttttatgcc tatataatcc
    12301 accgcattat tgtcatttta tttttgatat ccaatttttt cgtggtgtat atgtctaata
    12361 aaatctttac gcattcccta cctatgcgct atgccgattt tccaacgctg gttgatgctt
    12421 tggactacgc cgctctgagt agagccggaa tgaattttta tgacagacgt tgccaacttg
    12481 aagatcaact ggaatatcag acattaaaaa cgcgtgccga agctggtgcg aagcggttgt
    12541 tatcgctgaa cctgaaaaaa ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt
    12601 ttgtagaggc tttttttgcc tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc
    12661 caatgggcgt cggtcagcgg gattcctgga gcgccaaact ccagggttta ctggcaagtt
    12721 gccagcccgc agccattatc actggtgatg agtggttgcc actggtcaat gccgcgacgc
    12781 ataacaaccc cgaattacat gttttaagcc acgcttggtt taaggcatta ccggaagccg
    12841 atgttgcgct ccagcgtcca gttccgaacg atatcgccta cctccagtac acctccggca
    12901 gcacccgttt tccccgtggc gtcattatca cccatcgcga agtgatggct aatctacgtg
    12961 ctataagcca cgatggcatt aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt
    13021 tctaccatga tatgggactg gtcggctttc tcctgccccc cgtcgccacg caactttcag
    13081 tagattattt gcgcactcag gattttgcca tgcgtccttt gcaatggctt aaattgatca
    13141 gtaaaaatcg cggcaccgtt tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc
    13201 gcgtgaatga aaaagatctc gctgaactgg atctttcctg ctggcgcgtc gctggtattg
    13261 gtgctgagcc gatctccgca gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta
    13321 actttgacaa taaaactttc atgccgtgct acggactggc agaaaatgcg ctggctgtca
    13381 gcttctctga tgaagcctcc ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat
    13441 atcagggtaa agccgtcgcg ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact
    13501 gcggcaaagc gttgccggaa catggtattg aaatccgcaa tgaagcaggt atgccggtcg
    13561 cggaacgtgt ggtaggccat atttgcatct ccggtcccag tctgatgagc ggttactttg
    13621 gcgaccaggt ttcgcaagac gagattgccg cgacgggctg gttagacacc ggcgacctcg
    13681 gttatctgct ggacggttat ctgtatgtca ccggacgcat taaagatctg attattattc
    13741 gtggccgtaa tatctggccg caggatattg aatatatagc ggaacaggaa ccggaaattc
    13801 attctggcga tgcgattgct tttgttaccg cccaggaaaa aatcattttg cagatccagt
    13861 gtcggatcag cgacgaagaa cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc
    13921 aaagcgaatt tggcgtgacc gcggctatcg atctgttgcc gccccacagt attccccgaa
    13981 cgtcctccgg caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg
    14041 ccagtcttaa tgtgcaggaa tccctggcat aaatcaaact gtcgcggtga cgggcgctac
    14101 cgggtttatc ggtaaatata ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc
    14161 attgacgcgt actgcccgcg ctcacgtcaa tgataatctc acctgggtgc gcggttcgct
    14221 ggaagatact cattcactta gcgagctggt tgccggagcc agcgtagtgg tccattgcgc
    14281 cgggcaagtg cgcgggcaca aagaagagat tttcacccgc tgtaacgttg acggcagcct
    14341 gcgcctgatg caagcagcaa aagagagcgg cttttgccaa cgttttctgt ttatctcttc
    14401 gctggcggcg cgccatcccg agctctcctg gtatgcaaaa tccaaatacg tcgccgagca
    14461 acgactggca gcaatggcgg acgaaattac gctgggcgtt tttcgcccga cagccgtgta
    14521 tggtcccggc gataaagagt taaaaccgct gtttgactgg atgctacgcg gcctgctgcc
    14581 acgacttggt gcgccagata cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc
    14641 agtgggtcag tggttaagcg ccgaaactgt acagacgcaa acctatgaat tatgcgatgg
    14701 cgtcgccggt ggctatgact ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg
    14761 ttccgtgcga atggttgaca ttcctctgcc ggtactcaca ggccttgcgg atatcagtac
    14821 cgcgttgagt cgcctggcag gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt
    14881 aacccacgcc gactggtcgg caagtaataa ccgtatttct gaagatatca attggtttcc
    14941 cgggattagc ctggaacacg cattacgcaa cgggctattt tgattgtctt gttttaaagg
    15001 tgtgttaacg tttctatggt aaatcgtgaa atagtaatgg attacatcct ttcctgttta
    15061 caggatttgg tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt caacgatctt
    15121 ggcctggagt cgatcaaagt gatggatctc ttaatgatgc tggaagacag attcgatatt
    15181 tccattccta ttaatattct gctggatgtc aaaacccctg cgcaattaat ggaaactcta
    15241 cttccatggc tggagaataa ataatggggc tatacgataa atatgcgcgc cttgctggcg
    15301 agcgtctgca atttagtgac aacgggttaa caccgtttgg cacctgtatt gatgaagtgt
    15361 attccgccac cgaaggccgg atcggcaata aaaaagtgat tctggccggg accaataatt
    15421 atctcggttt aacctttaat catgatgcca tcagcgaagg tcaggccgcg ttagcggccc
    15481 aaggcaccgg caccaccggt tctcgtatgg caaacggcag ttatgcgccg cacctggcgt
    15541 tagagaaaga gattgccgag tttttcaatc gccctaccgc tatcgttttt caaccggtta
    15601 taccgctaat ttaggcgtca tcagcgcact tgccgaccat aacgccgtgg tgttactgga
    15661 tgccgacagc cacgccagta tttatgacgt ctgttctctc ggtggcgcgg aaattattcg
    15721 tttccgccat aacgatgcta aagatctgga acggcgcatg gttcgcctcg gtgaacgggc
    15781 gaaagaggcg attatcatcg ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct
    15841 ggcggaaatt gttgatatca aacgtcgtct cggtggttat ttgattgtcg atgaagcgca
    15901 ttcgtttggc gtgttaggcg caacggggcg cggactggct gaagctgtcg gcgttgaaga
    15961 tgatgtggat attatcgttg gtactttcag taaaagtctg gcctcgattg gcggttttgc
    16021 tgtcgtttcg gaagcgatgg aagtgctgcg ctacggcagc cgcccgtata tttttaccgc
    16081 ctctccttct ccgtcctgca ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca
    16141 accagagctg cggcaaaagc tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa
    16201 actgggttat gagttaagtt cacatattag cccggtagtg ccagtaataa tcggttcgaa
    16261 agaggaaggt ttacgcatct ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat
    16321 cctgccgcct gccgcgcctg caggcatcac gctgctgcgc tgtagcgtaa atgctgccca
    16381 cagtcgtgag caaatcgatg ccatcattca ggcatttgct actctgaagc agtaatgtga
    16441 gcccgccgtg gtgtttgcgc cacggcgggt ttaatgttgt ctgtcatacc aatacaccag
    16501 gctcaacgcc accaacacaa acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa
    16561 cgcaaatagc aatcccagat taaccatgat ttgatagctg atccaggtga gtaagcccac
    16621 gatgacgcct acagcgagac ggctacccat cccaggcgaa cgcggggcac taaaggtaaa
    16681 cggcacagcc agcaaaatca tcgccagggt caggatgggt tgccccagtt tttcccacag
    16741 tgcaaggtgg tattcgctgc tgggttgccc ggtttcctgc aaataatgga tgtaatgatt
    16801 aagctgacgg acagaaaaag tattccccgg cattgataac tcttcaagat tcatactggt
    16861 gaaggccgat tgccaggcaa gattatctaa tgtttccagc gtttctttgc cattcagcca
    16921 ttttttatga ttcacaccat gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg
    16981 tgcataaatg taggattcaa gcgagagatc gtcgcgataa tgaaatatct ccacgcccac
    17041 cggctggccc tgctcgttca gcgatttcac cgtcacaaat tcattaccac gcctggccca
    17101 cagcatattg ccggtaatgt catcgtcctc ccccaacgcg gtggcggtcg atttgatttg
    17161 caacgcctgt tgctgcaatg gcgacgccac ccactcatct atcgcgccta acgaaacagt
    17221 ccacaatatc ccggcaacca gcgccaccag tgcaatacgg aagatagaaa accccgtgct
    17281 gcgaatggcg gtaagctcac tgtttttcga taactgcccc agtccaacaa taccacccaa
    17341 taacgcgata aacggcgaaa gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag
    17401 caccgcctga gtccagcgat aaccgcccgg gctgacacca tccagttcgt taatcaggtt
    17461 gaaggtggta aaaagcggca gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg
    17521 acggattaaa tagcgactga aaacattcat cgggaaaatt tctgcagcaa agagaagtca
    17581 cgtgcgacca gtatcagcag gcccattagc atgagtccgg gcactaacca caaaccagga
    17641 atgaggggaa tcgcgccgtt agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg
    17701 gcaataaaca gcaacgtcag tggcaataac gttgaaaatc gcccttgccg cggttttacc
    17761 cgacttaatg aaatggccag cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc
    17821 cattgcagtt cggcatgatc ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc
    17881 gctttgcgtt tgacgttcgg gctttgatcc agcggattca gatgtaattg cagattacgg
    17941 tagatctgtt cgttgtcgtc acgcccctga tgatcgagaa gataggcagt gccgttgtgc
    18001 aacataacgg tcggtttctc gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa
    18061 atacgggttc gattggcagt agaagtataa atcagcgcgt cagtcaggcg attattatcc
    18121 tgatcaaccg tctgcgaaag gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc
    18181 cgcaactggc gaacatccag ctccgactgc gactgctgct ccagttgata aatctgcgca
    18241 taagcccacg gacgaccata catcgacaga agggtgacaa aaatacttag cggtatcgcc
    18301 agataaagaa cggctttgta caagcgtccc ggactgcccc ccgccgcaga gatagcggta
    18361 atttccgagt cggtatacat ctgccctagc gtcacgccaa ctgacacata cagaccaaca
    18421 ggtaacaaca tctccagtgc aatcagtact ttgtagaaaa cgatatccag cacaacatca
    18481 agagctaatg tcccatttgc cgcttcggtc agataacgct gtgcggagta gctggcgaaa
    18541 ataaagatca gaaaaccgac gataatcagc accagacggc gcgtaccacg catgatgtag
    18601 tgttcaacca gcttcatctc actaacgccc tcaatccttt gagtttttta ttcattgatc
    18661 agtttctgcg ccaacgcaat gacatgatct acctcttccg gcgtcaggct gccgtccttt
    18721 gcccactgaa tttgcccggc tttattcagc accagaatgg ttgaactctg ctccggcaag
    18781 cgccaggcaa cgcgtccctg tccattgccg tcaataacga attgcgccca cggatagcgg
    18841 cgtttatttt tttcgatttt gccaacaaca aaatagccag taccaaagat ggcatcatcg
    18901 gtgttcacaa tggtggtggg ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct
    18961 ttaattaaca gtgagttctt tttcttcgcc gacttacgcc cggcgatata ctgaataatg
    19021 cggacttttc ccgctaattg tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta
    19081 ttcagaagca gctcgccgcc ttcctggata taaacagggg taaccgtttt gccagtgacg
    19141 aaattgtgtg cctgcacacc cggggctaag agcatgataa tcaacgcaat aatcaaacga
    19201 gatgacatca ttgctccggc tatagcggaa aacgcgaaat cagcagctct aagcgctgac
    19261 aaatatcgct atccagcgcc aacggtgcat aatacgactg gcggttttcc atatcatccg
    19321 ccaccatgtc gtcgtgttta tctcccatca ggccattaat ggcggcgagg aaacgggaaa
    19381 tctgtaacgc ctctttattt tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct
    19441 gctggctgat gatttttcgc acatcatgaa attccctgcc ggttagtcgc ggtttggcaa
    19501 taatctcttt cagggtatgc atttgctttt gccgataggc aataaaactg cttacgcttt
    19561 ctaactggct atggcgtaat gaatagttca ccaacgacca gaccggagag cttaaataaa
    19621 tccgtaacag gttgccgtaa tacgacacaa tatttttctt accgttacga aagccgtcct
    19681 gaaaacgccc cagaaaaacg gtcgttttgc cgaataaaaa tccggcctga tgcttcttta
    19741 aatacagccg ttgcgcaaaa cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt
    19801 tgcgctcatc agcagtcagc tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt
    19861 cctgacgatc aacgccattg agccagaatt gcaggcataa cgcgtaatta tcatgaatga
    19921 tttcggtggt acaacttagt gcaatagtcg gcggcaaact aacctgtgca tcaacgacat
    19981 catcttcttc aatcgcaaca aatagacgct cgctcgcctc aagcgggaaa tattttctca
    20041 tcatcaggat ttctctccgg ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt
    20101 taaggttgga attaacgaga gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc
    20161 tgctcccagc acaccaaaca gatgacagaa catgaacagc aatacgatgt agccgattga
    20221 ggcgatggtt tgcgccacga gaaaagcgcg ctgttttccc gccatcaata gcaatgactc
    20281 ctgcgggaag cccagcatcg aaatcacaat tgcgcccagc atcacctgaa tcaggtcata
    20341 cgcttcgaga tacttaacgc caaacaccag cgaaatgagc ggcttgccga cgatcaacac
    20401 cgcgagcgcc accagaatac cgattccgcc cgccagtaaa ccggacttca cacctaacaa
    20461 ccacggtctg gtggtgcgcg gatctaaacg catcacctct gggtaaaaac ttttacccag
    20521 caaacccgct ggcgttccgg cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc
    20581 ggcagcgggt cctaacacga tccccaccaa cactgtgctg cacgagttac gcgccgacca
    20641 gatggagtgg gcaatgttgg ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc
    20701 agactcaaac agattcaatt tgaaggcgtt atggatattt ctacggcgta attcgcgcgc
    20761 ggcaaaccac cagtacatgg tgccgccaac cagattcgac acgtaccagg caataacaaa
    20821 acccacaaaa ccaaagtcaa aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg
    20881 tttcgtcgcc tgctgtacag caatcaaatc aaagcgatct accgcacgca gaatgccggt
    20941 cggcgtggag gaagccattg aaggaatgag cgtgcaatac agcgccgcca gccaaaagct
    21001 ttggtcatcc agacctaatg aatgggaaag gaatggcagt aaggcaatgc cgccgacaat
    21061 cgccaccgcg ccgctgacga tatccagcga gaaggaaaat gagacgacat tgcgaaattg
    21121 ctgcggatta ttgttggtca aggctggcgt tccgtactga accaccagct gccatgtctg
    21181 aaacttaata aaatcgctga tcgacttggc gtacgattgc acaatcacca gtacaccaaa
    21241 catggcgggc gtcatccctt taccggcaca cgagagcgcc aacagcccca gcaaggcgct
    21301 ctcgacatta ctggagccta accaggcgct attgcgaata atggtgcgaa acgcgccatc
    21361 tgcaaaccaa tgtttgatgt taaaacccgc caattcagcc tgaccttatc gttgatagta
    21421 aaaaatatcc cgccagcctt aagttaaact tcggcggtca gaaacgatgg caaccagaga
    21481 aaccgccttc tgtacctgtt ccagcacttc gctgtagggc gctctggaat caatctcaag
    21541 aatttttgtg ccgttatagc caatcgtcga catgacgccg attttgtcct gcagctcggc
    21601 atactcatgg tcaggcttgc gggagatggc agtatcaata tcaatcccca aacgaatgat
    21661 taattcagga cggtattgcg ccattttttg gtataaactc cgttcgcgct gcgccagaaa
    21721 catgctgatt ttcccggtcg cacgttcgac gccgatcccc ggtccgtcat aataaaagcc
    21781 cgaaatttca gcctgcggga agcgatcgct gaccaccaga acgccacttt gcgccagtcg
    21841 ctgaaccttg cgtagattcg ccattcttcg cagcgagaag cagtacataa tcaccgccgc
    21901 ccatagcgcc ggagatttgg ttttcatgct ttgggttttc gaggatttgg ccgccaatcg
    21961 ccgttccagc cagacgccaa ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga
    22021 gagcagcccc agatagcggc gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc
    22081 ggtggtcagc gtggatttac cggtgccatc acacccgacc accgcaatca gccccggaat
    22141 gtagttgggc tgcggcgcgg ttgtactatt aacagtttgt gtttgtagtg catccattcg
    22201 ttaaatcatc ccctatccat tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg
    22261 acgcttgcat aatttcatcg gctggttgcc gaccatccag ttcaaggatc ttggcgccat
    22321 tgaatgtcag ttgcggcgta acggcgattt tttcctgtaa cgctgccagt tggtggtcag
    22381 gtttacgcgc aaacgcggtt tgttcatcaa tgccaagacg aatcaacaat acgggtaaat
    22441 aagacgccat ccattggtac agcttcaact cgcgctgcct taacattttt atccaaccgt
    22501 taccgcccgt ggtttttgcc aattgcgggc catcaaagcg aaaacccggc acttcaacct
    22561 gcgggtagcg gtcggtgatg agcagatagc cttgctggct tttacacaac attttgcgaa
    22621 acttgtacgc ccgccagcag gaaagcagat agataaccag cgcagtaata ttgccaggcg
    22681 gagttaaggg cttttcgtgt acatgtgccg ctttacttcg cagataacac ccacaaggtg
    22741 cgccaataac agggagctgt gaaatccatt cgccaattcg cccggacgat tgcccgagat
    22801 aaatgtgttc tgttggcatt cttgctgcca gttcatttac caggcttgcc gtgagggtcg
    22861 atttacctga accatcacat cctacaatgg caataacacg cactggcgtg gaattaatta
    22921 ttgacatatt acgttgattc acgaaaaacc cggcagtaaa taatgtattg aatattacgt
    22981 ggtccgtgct cagaatatcc gttcaacctt attgtgacaa tgactaattg tgacattgac
    23041 ctgaattacg ctttcataaa aacatattaa ccaaataaat atttttaatg gatatttaaa
    23101 ttaaaggata tattcatgca gtcaataaca cctccattaa ttgccgttat tggtagcgat
    23161 gcttcaggca agtcaacggt gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct
    23221 gccgaaagag ttcatttagg aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta
    23281 cctttgatgg gaaaatcctt acataaaaca attgaacgaa atcaggtgaa aacagcaaaa
    23341 aaattgcctg gaccagttcc ggcgctggta attacagcgt ttgtcgcccg tcgcttactg
    23401 cgctttcgtc atatgcttgc ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat
    23461 cctcaggacc aaattcctgg cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt
    23521 ggtcgttttg tctcatggct ggcaagccag gaacgtaaag cgtttcactg gatggcgagc
    23581 cataagcctg atctggtcat caaactcaat gttgaccttg aagttgcctg tgcacgtaaa
    23641 cccgaccata aacgggaatc gctggcgagg aagattgcca taacgccaca gttaaccttt
    23701 ggtggtgcac aactggttga tatcgatgcc aatcagccac tggaacaggt gttggttgat
    23761 gcagaaaaag cgattacgga ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa
    23821 atgcccgatc gccaggaccg ggcattttca ggaaggttaa atcaactgca atgctatcca
    23881 gtacagccca ccagaaagaa aaattgccgc cggtaaagta aacacccacg ccatcaggat
    23941 gctggttacc gttttacgct gtaacccacc gccgtccacc accatcgtcc ctgctaccgc
    24001 agacgagagg acgtgtgttg tggagacagg catcccaata taactggcaa ggccgataga
    24061 cactgccgcc gtcatttgtg ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat
    24121 cttctcaccg atggtcatcg ctacacgacg ccagccaatc atggtgccaa tgccgagcgc
    24181 cagtgctacc gccatgatga tccacaccgg agcgtactca atggtgctta acatatcgct
    24241 gcgcagtttt ttcagcaggt tctggtcttc tttactgacg cctggcagtt tcgccagctt
    24301 cgcggaggta tcggagatgc acagcataat gcggcgcagc tggctgcgct gactcacgct
    24361 taacggctcg taactttcca tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc
    24421 aaaggtattt gccggatgac agtgaaactc tgttacttgc gtgccatcag tcgatgctgc
    24481 aggcaatgga ggttccatcg caatcaactt ctgcggcagt tcaggatgct gttgcaggta
    24541 gtgttcgaag ttggtaacgg catcgcgggt acgggtaatt tcatagccgg acgcattcat
    24601 attgacgacg aagccagcag gggcaatccc caccagtacc agcattacca ggccgatccc
    24661 tttctgcccg tcgttcgcgc cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc
    24721 aatacgcgtc cagaatggcg gtttacgttt gccctttttc tttttgcgat cttccggaat
    24781 gcggtgaata cggtcacgct ttttcgtccc actccagaaa cggcgcagca ggaaaatcag
    24841 accacccgca atgaccaggc cgacgatagg ggaaacaatc agcgaggaga aaattttggt
    24901 tacttcacgc aggtttaacg catccatcac cgatgagccg gttaacagcg cgttggttaa
    24961 accgatgccg ataatcgcac caatcaaggt gtgcgaactg gaggccggta aaccaaagaa
    25021 ccacgttccc aggttccaga taatcgccgc cagcagcatg gaaaagacca tcgccaaacc
    25081 gtgagttgac cccatattca gcaacaaatc gattggtaac atatggacaa tggcataggc
    25141 aacgctaagt ccgcccaata acacgccaaa aaagttaaaa aatgccgcca tcaccacagc
    25201 aagttgtggc tgcatggcac gagtataaat aacggttgcc accgcattcg ccgtgtcatg
    25261 aaaaccattg attgcttcgt agaacaacac aaatgccaga gcaagtaata acaaaagccc
    25321 cgtatatata tcaaggccaa caaataaatt tagcatatat tagattacgc cattttgaat
    25381 ttacgaacgg acgcattatc agtgacttta acggcatggg caaagtgaaa tatcattttt
    25441 ttaacgataa aaagagtgtt atttcttgtg tatcagacaa taagcattca ttcaaaacct
    25501 ttagaaataa tcaaaacaca ggtattccac tggttaatat attcacatat gaaatgaata
    25561 aatattggaa tatataaata ttgaatattt tgattaatcc ccgtactaat tattcttcat
    25621 aatcagtacg gggttgctac aacatgtatt actttttcac cacaattaac ggttcaatat
    25681 cactctcttt tttgatgacc agtgattcat caccgcgcaa acacgtccca ccgtagttgc
    25741 cgccaacggt gaaggtacat acctgaatgt atttaccgtc cactttcggc aaacaccaca
    25801 gttgctgata gatgtttttc tgctcggcaa atttaccgct ggttttgtcc agcacctctt
    25861 catgatggct gaccaggtcg atattgctac cacagcgacc ggcgatcggt ttcactgcgt
    25921 aacctgtttt caccaattca tcattaacag tgaaatcggt atccagcagg taacgatggt
    25981 gcgggaacag cgaccagagg atcggcagaa tcgctttgtt gccggggatc accgtccaca
    26041 gcggctcaaa gaccagcact tccgggcgca gcaatacgtc gataagacgc acttcgtttt
    26101 gcggatgacc ggtacggatt ggcaccgcag caaactcacg gtcgctaact tcacgaatct
    26161 gatcaaacgc ggtttcccac gcccaggttt tccacacgca gttaaccagt cgcccttccc
    26221 catcaatcag ttgcccggca gcatcccagc ccagttcatc cagcccgcgc aagatacgcg
    26281 tttcaaagcc cgcctggtgc agcgcctgct ccataaactg cgcgtgatag ttttcctcga
    26341 tatctttgtc ctgcatgata tggacaaacg gacgtgcacg actgtgtttc caggcaccag
    26401 ccagttcgtt aatcagccct tccgccggat tgaagccgtt gcctttatag ccctgctccg
    26461 cccaacgttc gaggatcaag cccgcttcgg tatgacagga ggcggagtcg gcgttgtatt
    26521 cgtaaacctt caggccacgc tcatccatgc agaaatccat acgaccagtg atcatatggt
    26581 gacggcgacg ctgccaggag agacgcaaac gtggccagag gattttcgga atgtcgaaca
    26641 gcgccagcag gttgtcatct ttcagcacct tgtcggttgc gtgaagatac atcagatgca
    26701 gctcgttggt ggctttaatt agctcctgct cggcactctc ggtaatggtg tagtaatgat
    26761 acggatcctg attgatcacc tgaccgttgg cctgcacata ggcgttttgt agcggatctt
    26821 tttcatccag ccatttaccg tcaaactggc ctttgttttc cagacgcgct ccactgattt
    26881 tcagcagctc gcctgcaatt tccggctgcg gtaagctgta ttcagtatct tccgtctgga
    26941 tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt aagggtatag cagccgtttt
    27001 cgaccaccat ctccagctcg cgcgtccact gttgcccttg cggcaacggg gaatgaatca
    27061 cgttctgttc cgcaatacgg actttgttgc catgcaattg ggtaatgatg gcgacatggc
    27121 cagtgtcttt aaattcaccg cctttatccc agataagaag cgcacccgcg accggcgcac
    27181 gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt gtcattaacc acttctcgca
    27241 ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc agtaaagacc acaccgtaat
    27301 tcagaaagag aaaacggcga gcaaattcaa cgcattgcca cttgtggccc atatattcgt
    27361 cgtcgatata gctacggaat acggcgtcat cttcgtattc ctgcggatcg agagaactgt
    27421 aatctgaaga gtagattgct accccacctg gggcgtagcc caataatgtc ccgaacgggg
    27481 catcctggct ggtcgttcct ttgctcatca ctttaccttt aacaatacaa cctaagcagt
    27541 tggcgtaatt gttgtgctct gattacctgc tcagcgacat taaccggaca gaggtcagac
    27601 taatcataca cctcatcgca gcggctggcg caaagggtta aaaaattcac attctgtaca
    27661 gcaagtgacc tgctacactg cttcaacact accactcaga aggcaactca ctatgacaga
    27721 caatacttat cagcccgcga aagtctggac gtgggataaa tccgctggcg gcgcgttcgc
    27781 caatatcaat cgcccgtttt ctggtccgac gcatgaaaaa acgctgcccg ttggcaaaca
    27841 tccattgcaa ctttattcgc tgggaacgcc gaacggtcag aaagtaacga ttatgcttga
    27901 ggagctactg gcgctgggcg ttactggtgc agagtacgac gcctggctga ttcgtattgg
    27961 cgatggcgat caattctcca gcggctttgt cgaagtgaac ccaaactcga agatcccggc
    28021 gctgcgcgat catacgcata atccgccgat ccgcgtgttt gaatctggtt cgatcctgct
    28081 ttatctggcg gagaaatttg gctacttcct gccgcaggat ctggcaaaac ggactgaaac
    28141 gttgaactgg ctgttctggt tacagggtgc cgcgccgttc ctcggcggtg gttttggtca
    28201 cttttaccat tacgcgccgg tgaaaattga gtacgccatc aaccgcttta ccatggaagc
    28261 caagcgcctg ctcgacgtgc tggataagca actggcgcag cataagtttg ttgcgggcga
    28321 tgagtacacc attgcggata tggcgatttg gccgtggttt ggcaacgtgg tgctcggcgg
    28381 cgtgtatgat gccgcagagt ttctggatgc gggaagttat aaacatgtgc aacgctgggc
    28441 gaaagaagtg ggcgaacgtc cggcagtgaa acgtgggcgt attgttaacc gcaccaacgg
    28501 gccgctgaac gagcagttgc atgagcgcca tgacgccagt gattttgaga caaatacaga
    28561 agataagcgt caggggtaag ggttggtgtt cgtcgcagca agccatccag gccggataag
    28621 gcgttcacgc cgcatccggc aatcgtgcac aatgcctgat gcgacgctgc cgcgtcttat
    28681 caggcctaca aaagctaatc atcacgcgct ggtaatgtcg taatccattt ggcgcagtgc
    28741 gtctaatgtg gctttggctt catcttcgtc gatgatgctc atggcaaatc cgacgtgtac
    28801 cagcacccac tggcccagca gatcggcagg gttaccttcg caaatcaggg cgatattcac
    28861 gtcgcgcttg ataccacata cttcaacctg cgcaagctgg tgaatatctt caccgacagc
    28921 cagcacctgg cctggaacgc caatacacat tgttaactcc ggttatttcg attgccggac
    28981 aaacgcgaaa aagaaccttt agcgggtgtt ccctctcacc ctaaccctct ccccggaggg
    29041 gcgaggggac cgtccgtgcg ctttttaggc gttgtcctca gtcttattca acttcaatac
    29101 ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga cagagcggac
    29161 actgcgcatc gtgctgatga atctccacca cctggctgca atcccagcac caggcctggg
    29221 cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt
    29281 caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcgccaatt tccagccaca
    29341 cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcgc
    29401 tctggcaaag cgacaactca tgcattttcg cccctcctgc ggccaaacag cagggcgcga
    29461 cgccctgcgt gtggtacatc cggattggtg actggcaacg aaaggatcat tcgtgcgcag
    29521 tcatccgtca ggcgttggcc ttcttcaatc gacatgctgt gcgaaagcgg cgacatcagc
    29581 gaacaggaga ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt catggtgcca
    29641 tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg
    29701 ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct
    29761 tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa agagagatcg
    29821 tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt cggggaggtc
    29881 tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt ccacgcctga
    29941 ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca atcattgctt caaccgtcgg
    30001 cgttaagccg atgtgtggct ccagcgattc cgggatcacg ccgaccaggg tcagcttttt
    30061 cggaaactca ccggtgaagc gcagggccga caatacgtcg gccaggccaa gctgatgcgg
    30121 agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca tcatcgttcc
    30181 cggagtgttc tttttcgaca caatggcatc cgcaataatc agatgatcgc gatttgccat
    30241 gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat aatccggcag
    30301 aatgtatcgt tgctctaacg cttcgacaat ccgcacgccg atggcttcat cggtcagcaa
    30361 aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg
    30421 ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg
    30481 gtacgcacca cttccagcgg tttattcgga tcggcaaccg gtgtacccac cagcgactgc
    30541 tcgtaaggac cgacgtcatc attgaagtta cgcggaccag agttccaggt tgatggaaca
    30601 accgcctggt agttgctgat gataccgtct ttaataacca tccagtgaga gagcataccg
    30661 cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat gttcggcttc
    30721 acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttggttttgc
    30781 aggatatcct gcaattcaca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag
    30841 tgcagctgtg ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca
    30901 ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat attagccagc
    30961 ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga atatttcccg
    31021 tcgtcagacc aaccatcata agccggaatg gtggtacctt cccacggcgc ctgtggcgct
    31081 tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag gtattcatcg
    31141 gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta gcctcccggg
    31201 aacaggaagc tgccgttttt gctgtcggtc gggaattccg gcacgctcag gtagttcacc
    31261 gcacctttac cgcgcgtcag ccattccggg tagaacgcgg cgataaccgc ggtatcaacc
    31321 ttataaacct gctcaacaaa gtcgctcagt ttgtcgatga aagacttgat gtacatcagg
    31381 cgctcaaggt tcaacacgcc caacccgtcg aggttgattg ggttcgcgac accacccaca
    31441 gccaggttct gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg gttagcgtca
    31501 cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc
    31561 agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg accgctggca
    31621 accagatctt tgatcttgtt ctgaacttta gtgaactctt ccggactgtt cagatgccag
    31681 gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt cagcttgcag tgcagaagtg
    31741 atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc atgcgtggtg
    31801 tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagc
    31861 gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac gccacagata
    31921 cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg
    31981 cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg
    32041 atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt
    32101 tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac
    32161 gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa atcaacagtt
    32221 cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc aacgaatagg
    32281 tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac agcatacggg
    32341 aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt
    32401 caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat
    32461 agataagctc gccaaagcgc agcccgatga aaatcgccag caacacactg atggtgttgg
    32521 tcagcttgac gaacaggctc ttttcatccg gaccgttgcc acgcagaccc gcctgcacca
    32581 gtgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca
    32641 gcggcaacat ttcatagctc tgccagagcg gatgcacctt gtagcccgcc gagatcatca
    32701 gcgaccccat tgaagactgg tgcatggtcg gcagcagcgc accgagcgcg atgatgaaga
    32761 acatcacctt gttcagacgt tgtagcgaca ccttccagcc cagacgttca aacagtgccg
    32821 gagcaaactc cagagccatc acgccgatgt agatggtcat acagaccgcc gtctcgaaca
    32881 gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac
    32941 ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc gccaacagcg
    33001 ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg
    33061 cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc cacacgcccc
    33121 acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga cgcttcacaa
    33181 tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt
    33241 tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt
    33301 ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg
    33361 cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt
    33421 ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg agtaccgccg
    33481 ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg
    33541 ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag cgccagacgt
    33601 tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc cgccgggcac
    33661 acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt ctggttgcac
    33721 agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg cacgttgtac
    33781 ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta atggacaatg
    33841 ccggttttcg gatctttttt cagtgcagag accgggcaca cagagacaca gttcggatcg
    33901 acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg
    33961 acccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga cagtttgtcg
    34021 ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat atcctgacac
    34081 ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat ccccagcgat
    34141 cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc
    34201 agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc
    34261 gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga
    34321 caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca gagacgttgc
    34381 cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt tcgacgttgg
    34441 caagctgatg gatgccttta tggaagccga tacctttttc gttacagcca tagcacgggt
    34501 gaccaatcgc caccggccac acaccgccaa catcgcagaa ttgcagcgtt gagcagttgc
    34561 cgtaagtttc tggcccttta cagccgaggt ggtacaagca ccagccttcg cggtggcctt
    34621 catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt
    34681 cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt ttcggcggtt
    34741 tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc
    34801 ccggaatatt gataacggtt ttgcctggca gaacttcttg caagctgact gcgccagttg
    34861 ggttaactcc agctgcggca acaccgcccc acgcggagca ggaaccaata gcgataatgg
    34921 ctgctgagcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg gcaaccatgc
    34981 aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca tactgccctt
    35041 tgtacttctc gagagcgtta tgtttgttct cttcgacctg atgaccgaag gcggcggaaa
    35101 gcacttcgtg atattccaga gagatagtct ccagcacgag gttttctacc gttggatgcg
    35161 ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg
    35221 gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc
    35281 ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag
    35341 aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaga acctggcata
    35401 tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat
    35461 tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag
    35521 tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc
    35581 cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc
    35641 tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa gctggcgatc
    35701 tccggtcttt tcgtacatcc tgtgtgagta gcctaataca aaaccgcgtt tataatcggc
    35761 acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa
    35821 gcctttacca aaatggttat tcatcgttca cctcattcat tatatatcgc catatataac
    35881 gatattttag gcaaagaacg acttttgcgc acgctatttt ttcatacttg tttaagacgt
    35941 aatcccgtaa gagataaacc tatcaggaga gatatttatc gaaccatttc agtgtccttt
    36001 gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta tggaatccgt
    36061 gattaacccc cggatagata taagcctcat aaaccttatt attgtctttc aacgccgcct
    36121 cgtaaggagg ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg agtagtaaag
    36181 gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac ggcaccgcgc
    36241 aggccagttc cggatacgcg acagccgccg cgttcgatac gccaccgcca tagcaaaatc
    36301 cggtaatacc cactttacct gtcgcttgcg gatagcgttg cataaactca attgcggcaa
    36361 agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga cctttatcat
    36421 catttcccgg ataacctcca acggaactta atccgtcagg tgccagggcg atataccccg
    36481 ccttcgccac tcgccgtgcc acatcttcga tatacggatt cagtccacga ttctcatgca
    36541 ccacccccac ggctggcgtt ttgccgctca tttttgcggg tttcaccaga taaccccgta
    36601 cctcgccgtg accatttggc gaaggatacg tgatgtactc agcaacaatc tccgggtcgg
    36661 taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac aacgctaatg
    36721 ccgtcatccc gcccaccgca tacttcgccg caaggttgag gaactcacgt ttcgaaattt
    36781 tcccgtgaac gtaatagtcg tagtaatcca acaactcttg tgggaaatct ttggcggtta
    36841 aacgcggcat cgttgcattc ctcaactggt gtttttttaa gcaaagcata agcgcgtgtt
    36901 tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg gcgaatcgct
    36961 aaactagaaa cattgtttcg aaattgaacg gtggaaagga gaggtcatgg tctggttagc
    37021 gaatcccgaa cgttacgggc agatgcagta ccgctattgc ggaaaaagtg gtttacgcct
    37081 gcccgcatta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc tggaatcaca
    37141 gcgtgcaatc ctgcgtaaag cgtttgattt aggcattacg cactttgatt tagccaacaa
    37201 ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc gcgaggattt
    37261 tgctgcttat cgcgatgaac tgattatctc taccaaggct ggctacgata tgtggcccgg
    37321 cccgtacggc tctggcggtt cacgtaaata cctgctcgcc agcctcgacc aaagcctgaa
    37381 gcgtatgggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg aaaatacgcc
    37441 gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg cgctgtatgt
    37501 cgggatctcc tcttactcgc cagagcggac gcaaaaaatg gtcgagttgc tgcacgagtg
    37561 gaaaattccg cttttaattc atcaaccttc gtacaattta ctgaaccgct gggtggataa
    37621 aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgtattgcct ttactcctct
    37681 ggctcaggga ttgctgaccg gaaaatatct caacggcatt ccgcaagatt cacggatgca
    37741 tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg cttaccgaag ccaacctcaa
    37801 cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga caatcaatgg cgcaaatggc
    37861 gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta ttgattggtg ccagccgcgc
    37921 ggagcaacta gaggagaacg tgcaggcgct gaataatctg acatttagca ccgaggagct
    37981 ggcgcagatt gatcagcata tcgccgatgg cgagctgaat ctgtggcagg cgtcgtccga
    38041 taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga atcagatcag tgattatgac
    38101 gagtcagtcg gtcaagatag cccatcacaa atgcagaaag cacaaacgtc agatggataa
    38161 tgacgtacca catcagtttg ttatccggga catttttcgc atccataaag acgcgcagta
    38221 agtgaatgga agaaattgcc acaatcgacg ctgctacttt gtttttcagc gacgttgcgt
    38281 ccattttccc cagccacttc agcttctctt tgttctcgga gatatccagc tgtgagacga
    38341 aattctcata accggaaaac atcaccatca ccagcaaacc gccaaccagc gtcatatcca
    38401 ccagcgacag caacacgagg atcaaatctg attccgccat cgagaagata ttcggcagta
    38461 cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc gaaaggccaa
    38521 agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga aaacgttcca
    38581 tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattgagt aaacagcacg
    38641 caacagcaaa tcaacatata cgttaaacct ttgtcgcata attctgaggt ttagggatct
    38701 tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt gaacctgaag
    38761 ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca gtattacact ggtgaatatc
    38821 ccaaacagaa acaaccgacg ccaagcatcc aggcgaagat gacaccgata ccggattgcg
    38881 gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagcactg gtgacagggg
    38941 gcgattccgg aataggtcgc gctgccgcca tcgcttacgc gcgtgaaggg gctgacgtgg
    39001 cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag atcattgaag
    39061 aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa ttcgcccgtt
    39121 cgctggttca cgaagcgcac aaggcgttag gcgggctgga tattatggcg ctggtcgccg
    39181 ggaaacaggt tgccattccg gatattgcag acctcaccag cgaacagttt caaaagacct
    39241 ttgccattaa cgttttcgcg ctgttctggc taacccagga agcgatcccc ctgctaccga
    39301 aaggtgcaag tgtcatcacc acttcgtcaa tccaggcata ccagccaagt ccgcatttac
    39361 tggactatgc ggctacgaag gcggcgattc tgaactacag ccgtggcctg gcaaaacagg
    39421 tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg ccctatctgg acggcactgc
    39481 aaatttccgg cggacaaacg caggataaga tcccgcagtt tggtcagaaa acgccgatga
    39541 agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt ttatctggca agtcaggagt
    39601 cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg cgagcattta ggttaaaaaa
    39661 atgcccggtt gtgaaaagca accgggcatc attgtgaatt cactcttacc tgatgtggcg
    39721 aaaacgcctt atccgcctac aaacgcatga agattcaaca tgttgcaata gattttgtag
    39781 gcctgataag cgtagcgcat caggcattga gcacctaatg ccggatgcgt cgtgaacgcc
    39841 ttatccggcc tacaaacgca cgaagattca acatgttgca atagattttg taggcctgat
    39901 gagcgtagcg catcaggcaa ttctacttta cttcgctttg gcggtttctt cgccgaccag
    39961 acctatcttc aggtaacccg cctgatgcag cgtatccatt accttcatca acgtctcgta
    40021 atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct ttcttgcctt cggttaacgc
    40081 attcaacgcc gtaatcattg tttcatcggt gaccggatcg ttaccgataa acatcgagtt
    40141 gtctgccttc accgacagat aaaccggttt ttccgggcgc ggctgcggcg tgctggtaga
    40201 agcaggcaag ttcaccttca catctaccgt cgctaacggt gccgccacca taaagataat
    40261 cagcagaacc aacatcacgt cgataaacgg cgtcacgttg atatcatgca tttcgccgtt
    40321 atcgtcgagg ttttcgttaa gatgcattgc catcggacat tatcctgcgc gtaatttttg
    40381 tgcgacacga accggatgcg cagcggcgct ggcttccaga tccaggtcac ggctttgcag
    40441 caacaatacc tgcgctgcaa catcacccag catcgcttta aagccgccaa tctggcgtgc
    40501 aaatacgtta tagataacga ccgcaggaat cgctgccacg aggccgattg ccgttgctaa
    40561 cagagcttct gcgatacccg gcgcaacgac tgccaggtta gtggtctgcg tttgcgcgat
    40621 accaataaag ctgttcatga tgccccagac cgtaccaaac agaccaacga acggagaaat
    40681 cgcgccgatg gttgccagat agccgttacc gcggcccatt tgacgaccca ctgcggcgac
    40741 ccgacgctcc agacggaagc tggtacgttc tttaatacct tcgttatcgt cgctgccttc
    40801 tgacagttcc agctcattct gcgcttcatt gagcaaatgc aggcttaagc ttttgctacc
    40861 aaaatcagcg gcgatatcgt tggcctggtt taaggaacgt gcttcagcca gcagttgctg
    40921 ctcgcgctta aggcgacgct tctgattgaa gaactctacg ctcttactga agaagattgc
    40981 ccaggtgact acagaggcca aaataagccc aatcatcacg cacttaacga caatatcggc
    41041 gtactgatac ataccccaga cggaaaggtc cgtctgcatt aaattattac ccactgtgta
    41101 tctccaggac gcaagtcaca aaatctgcgc ataataatat caaaacgacg tcgaattgat
    41161 agtcgttctc attactattt gcatactgcc gtacctttgc tttcttttcc ttgcgtttac
    41221 gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt ctgctttatg ccaattcttc
    41281 aggatgcgcc cgcgaatatt catgctagtt tagacatcca gacgtataaa aacaggaatc
    41341 ccgacatggc ggacaaaaag cttgatactc aactggtgaa tgcaggacgc agcaaaaaat
    41401 acactcttgg cgcggtaaat agcgtgattc agcgcgcttc ttcgctggta tttgagagta
    41461 tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg cgagttgttc tatggacggc
    41521 gcggaacgtt aacccatttt tccttacaac aagcgatgtg tgaactggaa ggtggcgcag
    41581 gctgcgcgct atttccctgc ggggcggcag cggttgctaa ttcgattctt gcttttgtcg
    41641 aacagggcga tcatgtgttg atgaccaaca ccgcctatga accgagtcag gatttctgta
    41701 gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt tgatccgctg attggtgccg
    41761 atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt tctggaatcg ccgggttcca
    41821 tcactatgga agtccacgac gttccggcga ttgttgccgc cgtgcgcagt gtggtgccgg
    41881 atgccatcat tatgatcgac aacacctggg cagccggtgt gctgtttaag gcgctggatt
    41941 ttggcatcga tgtttctatt caggccgcca ccaaatatct ggttgggcat tcagatgcga
    42001 tgattggcac tgccgtgtgc aatgcccgtt gctgggagca gctacgggaa aatgcctatc
    42061 tgatgggcca gatggtcgat gccgataccg cctatataac cagccgtggc ctgcgcacat
    42121 taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa agtggccgaa tggctggcag
    42181 aacatccgca agttgcgcga gttaaccacc ctgccctgcc tggcagtaaa ggacacgaat
    42241 tctggaaacg agactttaca ggcagcagcg ggctattttc ctttgtgctt aagaaaaaac
    42301 tcaatgatga agagctggcg aactatctgg ataacttcag tttattcagc atggcctact
    42361 cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc agaacatatc gccgccattc
    42421 gcccacaagg cgagatcgat tttagcggga ccttgattcg cctgcatatt ggtctggaag
    42481 atgtcgacga tctgattgcc gatctggacg ccggttttgc acggattgta taacattgcc
    42541 acttttggac aattttgcag acattttatt gtgaaaagtc ttaaattgtt gcgtccggga
    42601 tcaaggcgtc ccggacgatt caggagtaca ataggcagat aaaggcttaa acgctgttcc
    42661 acaggaaagt ccatggctgt tattcaagat atcatcgctg cgctctggca acacgacttt
    42721 gccgcgctgg cggatcctca tattgttagc gttgtttact ttgtcatgtt tgccacgctg
    42781 tttttagaaa acggcctgct gcccgcctca tttttgccag gcgacagctt gttgatactg
    42841 gcaggcgcat tgattgccca aggggttatg gattttctgc ctacgattgc gattctgacc
    42901 gccgcagcaa gtctgggctg ctggctaagt tatattcagg ggagctggtt agggaatacc
    42961 aaaacggtga aaggctggct ggcacagctt cctgctaaat atcaccagcg cgccacctgc
    43021 atgtttgacc gccacggtct gctggcgctg ctggctggac gttttcttgc atttgtccgt
    43081 acgctgctgc caaccatggc gggaatttcc ggtctgccaa accgccgctt ccagtttttc
    43141 aactggttaa gtggattgct gtgggtcagc gtggtaacca gttttggcta tgccttaagt
    43201 atgattccgt tcgttaaacg ccatgaagat caggtaatga cgttcctgat gatcctgcca
    43261 attgccttgt taaccgctgg cttgttaggc acgctgtttg tggtgattaa aaaaaaatac
    43321 tgtaacgcct gacgattttc cccgttcccg gctgctgtac cgggaacgta tttaattccc
    43381 ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc acaccgaagt aacgtttaaa
    43441 ctcacggcta aattgcgatg cgctttcata gccgacgcgc atcgctgctg cgctggcctt
    43501 catgccgtca tggatgatca tcatccgcgc cttatgcaga cggtaattct tcaaatactg
    43561 caacggcgag gtgctggtga cagacttaaa attatggtgg aacgccgata cgctcatgtt
    43621 ggcttctgcc gccagttgct cgacgctcag gttttcggtg tatttattct caattcgttt
    43681 cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg accagcgcca gtaacgcgcc
    43741 gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg atgatctgtt tgccgagaat
    43801 acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc tccgccgcgc ataaaatctc
    43861 ttctgataac gtggcggagt taatcccgct ggctgccatc gacggctgga aatgctcatc
    43921 ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata tcgacattga gacgcaaccc
    43981 tgccagcggc acctctgacg tggcataggt ttcgcactca aacggcaacg gcaccgtcag
    44041 cagcaggtat tcattggcat cataacgaaa cacgcgttca ttgatataac cgattttatg
    44101 cccggaaaag agaattatga tgccaggctc gtacatcacc ggtgtacgtg cgaaaggcgt
    44161 ctcgccatac aacaaacgca catcgggcaa cagttctgac aaactatttt ctttattttt
    44221 cagtttatta actttatccg ccagcaagcg acaaatctct tcacgtttca tatcgcgtaa
    44281 tttcttagga ataatgcggc aatttgattg tgcgcaattt tgtagcattt ctccagcact
    44341 ctggagaaat aggcaagaca ttggcagaaa tgagcattga gagccagggc gctggcgatc
    44401 acaatgaaaa acatcaggca gatcgttctc tgccctcata ttggcccagc aaagggagca
    44461 agtaatgaac aactttaatc tgcacacccc aacccgcatt ctgtttggta aaggcgcaat
    44521 cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta ttgattacct acggtggcgg
    44581 cagcgtgaaa aaaaccggcg ttctcgatca agttctggat gccctgaaag gcatggacgt
    44641 gctggaattt ggcggtattg agccaaaccc ggcttatgaa acgctgatga acgccgtgaa
    44701 actggttcgc gaacagaaag tgactttcct gctggcggtt ggcggcggtt ctgtactgga
    44761 cggcaccaaa tttatcgccg cagcggctaa ctatccggaa aatatcgatc cgtggcacat
    44821 tctgcaaacg ggcggtaaag agattaaaag cgccatcccg atgggctgtg tgctgacgct
    44881 gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc tcccgtaaaa ccacaggcga
    44941 caagcaggcg ttccattctg cacatgttca gccggtattt gccgtgctcg atccggttta
    45001 tacctacacc ctgccgccgc gtcaggtggc taacggcgta gtggacgcct ttgtacacac
    45061 cgtggaacag tatgttacca aaccggttga tgccaaaatt caggaccgtt tcgcagaagg
    45121 cattttgctg acgctaatcg aagatggtcc gaaagccctg aaagagccag aaaactacga
    45181 tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg aacggtttga ttggcactgg
    45241 cgtaccgcag gactgggcaa cgcatatgct gggccacgaa ctgactgcga tgcacggtct
    45301 ggatcacgcg caaacactgg ctatcgtcct gcctgcactg tggaatgaaa aacgcgagac
    45361 caagcgcgct aagctgctgc aatatgctga acgcgtctgg aacatcactg aaggttccga
    45421 cgatgagcgt attgacgccg cgattgccgc aacccgcaat ttctttgagc aattaggcgt
    45481 gccgacccac ctctccgact acggtctgga cggcagctcc atcccggctt tgctgaaaaa
    45541 actggaagag cacggcatga cccaactggg cgaaaatcat gacattacgt tggatgtcag
    45601 ccgccgtata tacgaagccg cccgctaagc tttttacgcc tcaaactttc gttttcgggc
    45661 atttcgtcca gacttaagtt cacaacacct caccggagcc tgctccggtg agttcatata
    45721 aaggaggaac gtatggctaa tccaaccgtt attaagctac aggatggcaa tgtcatgccc
    45781 cagctgggac tgggcgtctg gcaagcaagt aatgaggaag taatcaccgc cattcaaaaa
    45841 gcgttagaag tgggttatcg ctcgattgat accgccgcgg cctacaagaa cgaagaaggt
    45901 gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag aactgttcat caccactaag
    45961 ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc tcgacagcct gaaaaaactc
    46021 cagcttgatt atatcgacct ctacttaatg cactggcccg ttcccgctat cgaccattat
    46081 gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg gattaatcaa aagcatcggc
    46141 gtgtgcaact tccagatcca tcacctgcaa cgcctgattg atgaaactgg cgtgacgcct
    46201 gtgataaacc agatcgaact tcatccgctg atgcaacaac gccagctaca cgcttggaac
    46261 gcgacacaca aaatccagac cgaatcctgg agcccattag cgcaaggagg gaaaggcgtt
    46321 ttcgatcaga aagtcattcg cgatctggca gataaatacg gcaaaacccc ggcgcagatt
    46381 gttatccgct ggcatctgga tagcggcctg gtggtgatcc cgaaatcggt cacaccttca
    46441 cgtattgccg aaaactttga tgtctgggat ttccgtctcg acaaagacga actcggcaaa
    46501 attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc ctgaccagtt cggcggctaa
    46561 cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg acttttcaga aatcatttat
    46621 tcccctcgcg tcccgcccgt tgttactctt ccttgttcag gaatgccaaa tataaggaca
    46681 tcatcatgca gagccggaag ctcttaaaag aacaactcat ctatatccgg gataaacgca
    46741 acggagaggt gaaaaacaga tgaaaataat acttctgttt ttagcagccc tggcaagttt
    46801 taccgtacac gcgcagcctc cttcacagac cgtagaacaa acagtccggc agatttatca
    46861 gaactataaa tcagatgcca gtacgcctta tttcggtgaa accggagagc gggcgataac
    46921 ttcagcgcgt attcaacagg cgcttaccct gaacgacaat cttacactac cgggcaatat
    46981 tggctggctg gattatgatc cggtttgtga ttgtcaggat tttggcgatc tggtgttaga
    47041 aagcgtagcg ataacccaaa ctgacgtcga tcatgccgat gccgttgtgc gctttcgtat
    47101 ctttaaagat gataaagaaa agaccacaca gacactgaaa atggtggcgg aaaatggtcg
    47161 ttgggtcatt gatgatattg tcagcaatca tggcagcgtc ttacaagcag ttaatagcga
    47221 gaatgaaaaa acgctggccg ctttagcttc gttgcagaaa gaacagccag aatcctttgt
    47281 tgctgaactc tttgaacata ttgctgatta tagctggccg tggacgtggg tggtttccga
    47341 ctcttaccgc caggcggtta atgccttcta taaaaccacc ttcaagacgg ccaataatcc
    47401 cgatgaagat atgcaaatag aacggcaatt tatttacgac aatccgatct gttttggcga
    47461 agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg gagaaaaccg ccgattccgc
    47521 ccgcattcat gttcgtttta cgctgaccaa tggcaacaat gaagagcaag agctggtttt
    47581 acagcggcgc gaaggtaagt gggaaatcgc tgattttatc cgcccgaaca gcggcagcct
    47641 acttaagcag attgaggcaa aaaccgccgc cagattaaag caatgagctg aattaaataa
    47701 caattagccg gaacaataaa taaaagggaa cactatatga aaacgatttt caccgtggga
    47761 gctgttgttc tggcaacctg cttgctcagt ggctgcgtca atgagcaaaa ggtcaatcaa
    47821 ctggcgagca atgtacaaac attaaatgcc aaaatcgccc ggcttgagca ggatatgaaa
    47881 gcactacgcc cacaaatcta tgctgccaaa tccgaagcta acagagccaa tacgcgtctt
    47941 gatgcacagg actattttga ttgcctgcgc tgcttgcgta tgtacgcaga atgataaaaa
    48001 aatccccggc agcatgtcag ttgccgggga ttttttttaa cgtccaaccg ccgctttagg
    48061 gcgtttcttc gcaccagcat tcaccggacg agattgcgta gacgacgctt tttttgccgt
    48121 agcaggcgtc tgacgctgag tcgccatcgg cgtatgttta gtcaacgccg gacgggtatt
    48181 gcggttctgg cgacgagctt cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc
    48241 gcgacggctg ccaatcagat gctttttgcc catcgcttcc agcgcctggc ggattaacgg
    48301 ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct gtttgtcgcc
    48361 cttcggtacg aagacgcctt cgctcttata accaatcttc gccagcggtt ttttcccggt
    48421 gtaatacatg gtggttgagt tcgccagcgg cgatgggtag aagttctgta cctggtcgag
    48481 acggaaacga tgctttttca gccacagcgc caaattcacc atatcttcat cacgcgtacc
    48541 ggggtgcgca gagatgaaat acgggatcag atactgctct ttacctgcct gtttcgagta
    48601 ggtatcgaac agctctttaa agcggtcata gctgcccatg cccggcttca tcatcttcga
    48661 caacggccct tcttcggtat gttccggagc aatcttcaga taaccgccga cgtgatgggt
    48721 cgccaactct ttgatatagc gcggatcttc cacggcgatg tcataacgta cgcctgaggc
    48781 gataaggatt tttttaatgc ctttcagatc acgcgcacgg cgatagaggt tgatcgtcgg
    48841 ttcgtggtta gtgtccatgt gcggacaaat atccggataa acgcacgaca aacggcgaca
    48901 agtttgttca gcgcgtggcg atttgcagcg caacatatac atgttggcag ttggcccacc
    48961 gagatcggaa atcacgcccg taaaacctgg aacggtgtcg cggatcgctt cgatctcatt
    49021 aatgatcgaa tcttccgaac ggctctgaat aatgcgccct tcgtgctcgg taatagaaca
    49081 gaaagaacag ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga tcatttcgta
    49141 agccggaata cgggcattgc cataggccgg atgtggcacg cgcttgtacg gcagcgcaaa
    49201 aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc gggttgatcc acacatagcg
    49261 gtcaccgtgt ttttgcatca atgcgcgggc gcagcctggg ttggtttcgt ggtgcagaat
    49321 gcgcgaagca tgggcgtaca gcactttatc gcccttcact ttctcgaaag aaggcagcaa
    49381 tacgtaggtt ttttcccacg gtttctggcg cggtggctgc acgattacag ctttcgcttc
    49441 ctgctttttc ggcgcaacag gtttgttatc cgcacacggc aaatcttcgc catacggatg
    49501 cgggattggg tcgattttcc ctagggtatc aagacgggtg gaatccacac cgctccagcc
    49561 aggcagcgcc tcttttacga taattgcggt attacgcaca tcgcggattt cactaatcgg
    49621 ctcgcccatc gccagacgat gcgctacttc caccagcgga cgttcaccgt taccaaacat
    49681 cagcatgtcg gctttcgaat ctaccagaac ggaacggcgc acggtatcgg accagtaatc
    49741 ataatgcgcg gtacggcgca ggctagcctc aataccgccg aggatcaccg gcacatcttt
    49801 ccacgcctct ttacaacgct gggtataaac cagcgtggcg cgatccggtc gcttacccgc
    49861 gacattatct ggcgtgtagg catcatcatg acgtaaacgg cgatcggcgg tatagcggtt
    49921 gatcatcgaa tccatgttgc cagcagtaac accaaaaaac agattcggtt tacccagacg
    49981 cataaagtcg tctttgctgc tccagtccgg ctgggcgatg atcccgacgc gaaagccctg
    50041 cgcttccagc atacgaccgc aaatcgccat cccgaagctt gggtgatcga catacgcgtc
    50101 gccagtaacc aaaatgatgt cgcagctatc ccagccaagt tgatccatct cttcacgaga
    50161 catcggcaaa aacggtgccg gtccaaaaca ggcggcccag tactgcggcc aggagaacag
    50221 gtcgcgatcc ggttggatca gggagataga gctcattttg cttccagaaa tgataaaaaa
    50281 ataatcaaag gccggggatt ataagccgga acgaaagaga aatcgaaagg tattccatac
    50341 tcgccctcct cgggcgagta tgaagattac ggtaccggat tgaccaacag ttgcccaatc
    50401 gagccacggt ccgccatttc cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag
    50461 gaaggctgac cgaaataaac aagcagctcc acttgtccgt caacccaaac ggtatctttc
    50521 cagcctctgt cttccgggaa cggcatcgcg ccattcacgt tacggatctg gaacattacg
    50581 ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct
    50641 tgctgcgcgg tgacatcaat acggttgacg tcccacagct gtccattaat acccgggtca
    50701 tcacccagac tgatatcgcg actgcgaatt ggcgaaccag ccatgatttc agttggcagc
    50761 aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc
    50821 accaaggtag aaaccagaat actggatggc tcaaagaagc cgcgaatacg atcaacaatg
    50881 ctcgccgctt caccacaggt gatcgacact tcatcgccgt tgctcatatc caccagaatc
    50941 tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc aggcaggaat
    51001 ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc tcatctgtag ttgatagcga
    51061 cgagagttcg acgcgttcag cagtcgcaag cgcacccagc cacgcgagac ttcaacgtac
    51121 gggctttgta caccgttaac cagcagcgta tcaccaacaa agccgccgct tcccggttcg
    51181 ttgtattctg gcgtaccaaa gttatccagc cgtttatcct ggataatgac cggaaaatca
    51241 tccacaccat aatggttggg gataggcagc gacttgctga cttcatcttc caccagccac
    51301 attccggcaa ggccgttata gacctgctga gccgtgcggt tgggagtatt ggcgtgatac
    51361 cacagagtag ctgcgttctg gcgaataggc agtacgggtg cccagtcagc gtttggcgac
    51421 atcatccgtg ccggaccgcc catcagcggg cctggtacct gtagcccggc caccgtcatt
    51481 gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc cttccagacg
    51541 cggatagtcg gccccaggta acgaccattg attccccaga ccgacgcgcg tgtccctggc
    51601 gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg gcgagattca
    51661 agtagcggcg gaacgggtag cggttgctgt tgcccggctg cgctggcctt caggggaaca
    51721 gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact gacgccgact gagtgacata
    51781 tttgctccat gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc ccgcaaaaat
    51841 acagattgcc gacttaaact tttccggcgg cttcgcgttc tgcgacttct ttatcgagct
    51901 cggcgatttt ttgttccatt atcgaacgac aatgggcagc cagctcacga acctgatctt
    51961 tgccatactg actgacgtca attggcggca gcatttcgac aatcaccaga ccgttgtgca
    52021 gtcgattaag attaatctta ttcgaagttg tagagacgca cacgggaata atcgggacgc
    52081 ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg tagcaggccg cgaccacggc
    52141 tgcgggttcc ttccgggaac atccagatgg aaatacggcg ttttttgaaa tgattcacga
    52201 cttccgcaat ggtgccgtga gctttagtgc gattgtttct gtcgatcaat aagttgccgg
    52261 ttaaccagta caactgcccg aagaagggga tccacagcaa gctcttttta cctaccgtca
    52321 ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc tggtggttag
    52381 cgatatagat agcattgccg tagctttcag catctgcagg tttgcggcac tcaactttca
    52441 ggccgaacag tggcgcaaga cggccaaaca tatgcccaaa ggtggccaca tgtttcgggt
    52501 tacgcgggct gaaaaggcag taaatggagc cgaatacaca gactaagatg ctgtaaatca
    52561 cggtaataat aagacgaaag atatatagca taacaccctc tgaaggtctg agagactggc
    52621 attgtacgtc acctgaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt
    52681 attgttaatc gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg
    52741 ggttgaatac gctgccggat gacgacttaa cgtttcatcc ggcgttcctt gctagcggga
    52801 ggaaacagcg ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg
    52861 agagtcgatc tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc
    52921 acgttcgcca gtgactttct gtagctcttc cggacgcagt ttaattttgc gtttcccaac
    52981 atgaatggtc agcgtgcttt gcggcggcag aacgtacagt tgcgccagac catcctcgcc
    53041 acgcgcggct tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg
    53101 cggcagatcg cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat
    53161 atcggaagca tcttcaatca ccaccggcgg cataacatgg gcattttccg gtaaggtgat
    53221 caaagcctta cccgcacggt tacgcgctac cagatcgtta aaggtgcaga cgaaaccgta
    53281 acccgcatcg gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac
    53341 ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt tcgccctgac cacgcgccga
    53401 cggcagcgta atcgggtcga tggcatagct acgaccggtg gaatcaacaa acactaccgg
    53461 ttggttactc ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc
    53521 cggcgcgtcg atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat
    53581 ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc
    53641 ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt
    53701 cagcaggtta ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt
    53761 ttccagctca ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag
    53821 tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc
    53881 cggtttcggt tcatcttcgt tacgaatgat ctcaatcact tcgtcgatgt tgagaaacgc
    53941 caccagcaaa ccttccagga tatgcaggcg cttgaggact ttctccagac gatagttcag
    54001 tcggcggcgc acggtatcgc gacggaacac cagccattcg gagaggattt ccagcaggtt
    54061 tttcaccgcc ggacgaccat ccagaccgat catattgagg ttaatgcgat agctcttttc
    54121 cagatcggtg gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg
    54181 cggcacaatc accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac
    54241 catcggcagc tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga
    54301 aacctgatgc ggtaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg
    54361 catacgcact gaaccacgtc cgttctcgta gattttacga atctcggcgc gcgaagtgat
    54421 aatttccgct tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt
    54481 agttttcggc tggtcgatta atgcgattgc cgcctgagct acttcacgca ggttatgcgg
    54541 tggaatatcg gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg
    54601 cagacgggca ggtagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc
    54661 agccgtcccc tgccccagct cgctcaatag cagctcggaa tatttcgaca accgggattc
    54721 ggtgtaacgc attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc
    54781 atcaaccagc ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca
    54841 ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt
    54901 tttaaatttg gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg
    54961 aacaggtttc agaccatcac caataaacgg caacgcacgg tccatgatca cgtacatgga
    55021 gtagtttaag taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct
    55081 cattaattct gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat
    55141 actacattat ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg
    55201 ctccgacggc gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacttga
    55261 tcggcataga gggcagcaca aggttgcgcc catgccacac cactcggcat gggggtccgc
    55321 tccgggcgat tcgagaagtt gaggttatgc gagtccaggg aagcctaaag catcaaagta
    55381 gtcgactgta agtatacggt tcagcccagt ttagctgtgt ctccaccagt gccccgcgcc
    55441 tcctgccata agtgcggctt gctcatggat atccccgaga cggcttacgg cgcaccggat
    55501 aataaatttc gcgtatacat ggtattcctt accggagaaa ttataagccc aacaacaata
    55561 cgcctgtgac attgttcgct caaacttatc gcgagtgatt tggctcacac tccggtaaag
    55621 attccccatc acgccaacgg cacataaaca tccgtttcca gtcgctgctc tgtcacccct
    55681 tcggcaagat tggtgtagtg aaacagaatc ggtgctttac gcattttctc gccgcttgca
    55741 ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaacg
    55801 tggcgggcca cggcataacg tccaccggta agttcaccat tgctcacacc ataacgatta
    55861 tcgggaatca gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg
    55921 gtggcagggg cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc
    55981 gtctctttac gccacatgat aaatttcgcc acactgtagt tgagcaactc agggctacag
    56041 cggtgcggca acatcgccac agggattgat gggaaatcga taatgtttac atccagtgtc
    56101 aggtttgtca ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa
    56161 caattacgga tttactttgc ggatctgttt gacgtcaatt tcaacagaat tccagtcttt
    56221 atcgacttca ccctgaatct caaccgtatc tttcggcgtc accgtcacac cgttccagcg
    56281 tttgtgatcg atatcaacat tgatagtacc gctggcatct ttaaacacgt agagatcgtc
    56341 agagatgcgt tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt
    56401 tgcgctttct acagtcgtta cgctgccgtt cggcccctga aatcctccgg cctgactttg
    56461 cgttaccgat gggccagaaa aaccgccctg ctctgctgcc atcaccggtg cactgcacag
    56521 ggccattact gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg
    56581 tttccgagca tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta
    56641 atgctgtaca gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga
    56701 tgaaaaaatg cgaattttac tgatagaaga tgacatgctg attggtgacg gcatcaaaac
    56761 tggccttagt aaaatgggtt ttagtgtcga ctggtttaca caaggtcgtc agggaaaaga
    56821 ggcgctttat agcgcacctt atgatgcggt gatcctggat ttaaccttac caggcatgga
    56881 tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag cgtgagccgg tactgaacct
    56941 gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg cgtctgggag ctgacgatta
    57001 tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg ctggaggctc tgatgcgccg
    57061 aaccaacggc caggccagca acgagctgcg ccacggtaac gtcatgctcg accccggcaa
    57121 acgtatcgcc acgctggctg gcgaaccctt aacactgaaa ccaaaagaat ttgccctgct
    57181 ggaattactg atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact
    57241 gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg
    57301 acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga
    57361 gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc tgacgctaat ctttttaatt
    57421 ctggcctcgg tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac
    57481 gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca aacggttaag tacgctcgat
    57541 ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt
    57601 catgttgatg acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt
    57661 aatgatggcg ataacggaga agatattccc tatagctatc aacgggaagg ttttgctgac
    57721 gggcaactgg tcggtgaaga cgatccttgg cgttttgtct ggatgacctc acctgatggc
    57781 aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg aagacatggc gctggcgatt
    57841 gttgccgggc aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg
    57901 gtactactgg gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt
    57961 gaccctgact cggaaaaacc actaaacgcg actggcgtac ccagcgaagt gcggccactg
    58021 gttgagtcgc taaatcaact gttcgcccgc acacatgcga tgatggttcg agaacgacgc
    58081 tttacctccg atgcagctca cgaactacgt agcccgttaa cagcgctgaa agtgcaaacc
    58141 gaagttgcgc agctctctga cgatgatccg caggcgcgga aaaaagcact gctccaatta
    58201 cattccggga tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg
    58261 gactcactgg ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctacaatcg
    58321 tcggtgatgg atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc
    58381 aatgcccacg gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat
    58441 ttgctggata acggccccgg tgtgacacca gaggcactgg cgcgaattgg cgaacgcttc
    58501 tatcgcccac ccggacaaac cgctaccggc agtggacttg ggctatcgat tgtccagcga
    58561 atcgccaagc tgcatgacat gaatgttgag tttgggaatg cggaacaagg tggatttgag
    58621 gcgaaggtaa gctggtaact tctctgtcta tgagagccgt taaaacgact ctcatagatt
    58681 ttactaatag caaaatataa accgtcccca aaaaagccac caaccacaaa ccagacaaat
    58741 aacgagcgag aatacatata gccaaaactc gaacatttcc cgcataaaga gttttcttaa
    58801 gataagaata ataagtggcg taagaagaaa aaatgctgca atcattgctt gcaatatacg
    58861 atcttcgcct gaaacggaag gtatactgca aatattgcct cccgactcaa gcatccagtc
    58921 atacttattg aaagcaaaaa aagaatctat taaccaataa gtaagcatta tatatagcaa
    58981 tgcttcaaat attgttttaa ttttctgctt taacatatat cagtacgctc ataaaaatcc
    59041 atttataatt atcaacatga cacgttgata ttttcatctt ttacttattc aaagccatat
    59101 tttcaatccc caaaaaaacc gaaccaaacg tgtaaattat tccggtgcga gtaagaatta
    59161 tgaggaatgg ctatcagtat tgtcattttc agaaaatatt tatcctgcat cggtgagtca
    59221 gagctagatc agacttttgc taaattcgca aaagactttg cacattttgc taatttcaca
    59281 gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgagatta aaaaatgagc
    59341 aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac
    59401 accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt ccgcatcgtt
    59461 cgcgccgaca gcgactacaa tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg
    59521 gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt
    59581 gatgatgtat tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa
    59641 gatccgtcga aaaaatacgg ttccggcagc ctggtacagg acaaaaaata tatgctttct
    59701 ctaacctgga acgcaccaat ggaagccttc accgaaaaag atcagttctt ccacggcgtt
    59761 ggcgttgacg gtgtgtatct gccgttccat aaagcaaacc aattcctcgg tatggaaccg
    59821 ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg ctatactgaa
    59881 gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct ttagaaggag
    59941 ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag
    60001 gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac
    60061 ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat
    60121 tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca tctgcaaacc
    60181 ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat gaatatccgt
    60241 attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac cgggcaatgt
    60301 tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca gttgcggcga
    60361 tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg ctgatcgtct
    60421 tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc
    60481 aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg cttgcctttc
    60541 ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc
    60601 tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc
    60661 accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca
    60721 tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct cagatcgtcg
    60781 ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga ggccagcact
    60841 tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat gatcgcctga
    60901 tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacgaggaac
    60961 agttcggtac ggttaaggcc ctgcgcggta caatcagcca gtttgccagg cagcgccggg
    61021 ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg ctgggcgctg
    61081 gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag ccacaggatg
    61141 aaggcatctt tcaccacgcc cgaaacgaat gccgcacact gacgcgaaga gagacgctct
    61201 ttggtctgcc cggcaaactg cggatcctgc attttaaccg acagaacata ggcgcagcga
    61261 tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg gtattcacag
    61321 aactcacgca tcgcgtccaa caggccctga cgcagaccat taacatgggt accgccctgc
    61381 atcgttggga taaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac
    61441 agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa cggtttttct
    61501 ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag
    61561 caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac gccggggcac
    61621 agtactgctt tggccttcag cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca
    61681 aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg
    61741 ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt ataaacctga
    61801 ccatcgcggc gcacgttaac ttctacgcgc ttcgacaagg cgttaactac cgaaatcccc
    61861 acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc gcctgcatgc
    61921 agacggcaaa gaatcagttc aaccgccggt actccttctt ccgggtgaat atccaccggc
    61981 atcccgcgcc catcgtcaat cacttctaac gactggtcag catgtaagat tacatccacg
    62041 cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa
    62101 tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg
    62161 gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt tagcaattcg
    62221 agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc
    62281 gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcatagta
    62341 cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa
    62401 atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc
    62461 tagcacatat tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt ccgtcagcaa
    62521 ttcaaacggg cgtaccgccg ggtttaccac cactgcgggc agcataaaac attgcgacaa
    62581 ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg
    62641 ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg gcaactgcgg
    62701 aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca agctcgcttt
    62761 tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct
    62821 tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc
    62881 gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc cagcgtaaag
    62941 ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg
    63001 ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga
    63061 aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg atcgagccaa
    63121 ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca
    63181 tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg
    63241 ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa cacccgcttc
    63301 gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg
    63361 cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag
    63421 gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc
    63481 tggtgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt tacccctaac
    63541 agggcttcgt gcttttgtgc aaacaggtga gtatcggtaa tttgtaaaat ccttcctctg
    63601 gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga
    63661 cgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct
    63721 aaaaactggt taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca
    63781 taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca
    63841 tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt
    63901 tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg
    63961 tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc
    64021 aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc
    64081 ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg
    64141 atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg
    64201 cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat accgcttgcg
    64261 gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc ccccgggctt
    64321 gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc ttcaatcgct
    64381 tcgcgacggg caacatcttc cacactttca ccctcttcaa tcatcccggc aaccatctcc
    64441 agtagccagg gggtttcgct ggtgtcgtac gcggcaatcc gaatctgctc aatcagcaca
    64501 acttcatcac gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt
    64561 tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa tctataaaga
    64621 tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg
    64681 ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctagc actaatagtg
    64741 aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatat gttaaattga
    64801 ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga
    64861 atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa
    64921 tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt tcgttgagcc
    64981 aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta
    65041 agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac
    65101 tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg
    65161 gcatcaactc gaacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt
    65221 cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac gtcacatatc
    65281 agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg
    65341 ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt caattagatc
    65401 aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg
    65461 cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg
    65521 tagagcagct gcgccagatc accggtaact actatccgga actggcggcg ctgaatgtcg
    65581 aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac
    65641 gcaacctgtc gctgttacag gcacgcttga gccaggacct ggagcgcgag caaattcgcc
    65701 aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg atttctgaca
    65761 cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gatagcaata
    65821 tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta
    65881 actcgcaggt gaaacaggca cagtacaact ttgttggtgc cagtgagcaa ctggaaagcg
    65941 cacatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat gcttctatca
    66001 gtagtattaa cgcctacaaa caagccgtag tttccgcgca aagctcatta gacgcgatgg
    66061 aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg accaccacgc
    66121 tgtacaacgc caagcaagag ctggcgaatg cgcgttataa ctacctgatt aatcagctga
    66181 atattaagtc agccctgggt acgttgaacg agcaggattt gctggcactg aacaatgcgc
    66241 tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg
    66301 ctattgctga tggttatgcg cctgatagcc cggcacccgt cgttcagcaa acatccgcac
    66361 gcactaccac cagtaacggt cataaccctt tccgtaactg atgacgacga cggggcttcg
    66421 gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt
    66481 ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga
    66541 agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg
    66601 cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg
    66661 aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc
    66721 caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta
    66781 ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgtc
    66841 agcaagcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggct cagcaatcca
    66901 gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg
    66961 gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccggccagt ccggcttacg
    67021 gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga
    67081 ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac cactaccgtt acccgtggcg
    67141 gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt
    67201 ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg
    67261 gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg
    67321 gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg aagaagtcac
    67381 cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct
    67441 gatgaccaaa ttccgcatac caaaacacac ctggagtttt gtgcgtcaat catggctgac
    67501 tcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa
    67561 acttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg
    67621 gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa
    67681 cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca
    67741 gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt
    67801 gcaggactgc gcaaccgaag ctgaaatcgc caccgagttc ctctacatcg acgagatagg
    67861 tttaggcgaa aaaggccagt tcacggattt acaggatcag gtgatttcta acctgttcaa
    67921 gctttatccg tgggaattta tgctgcgcga aatgttctcc accaagctgg aagatgctgg
    67981 cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaggcac ttctaccgct
    68041 actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga
    68101 tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt
    68161 gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg
    68221 gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg
    68281 tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcgttgat
    68341 cacccaggat atgtctcggt tttatccaca tatttttgtt gaataagcca cgataccgga
    68401 tggcactcgc catccggtaa ttgttagcct atctgcaccg acagcatact taaactgccc
    68461 atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacac acctaacaca
    68521 tacaacaacg gcagatagtg ctctggcgtt gggttcaata acgcgccacc ttcatggtcg
    68581 agataattca ccagaggatg ctgctctacc ggcccttgcc acgtcagatt cgctttcaca
    68641 tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca cttcactgtg
    68701 cgcaggttat gcaccacgtt accgctggcg accaacatta ttccttcatc acgcagcgcc
    68761 gcgagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac
    68821 tgcaccatag ggatatcggc gtcaggatac atcttaatca gcacgcccca cgagccgtgg
    68881 tcaaagcccc aggcttcttt atccagcgtc accgggatcg gcgctaacag ctcaaccaga
    68941 cgctgtgcca gcgcaggcga acccggagca ggataatgcg tatcgtacag cgcctgcggg
    69001 aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc tgttccacgg
    69061 gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt catccccaac
    69121 ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct
    69181 aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt
    69241 ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga
    69301 tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca
    69361 gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc
    69421 gttctcggac aaaaaccctc gaaccgctta ctggcgtttt cgctaggttt tgcggcgggg
    69481 atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc tgaaggaatg
    69541 tcgccggtgt tgggttatgg aatgtttatc ttcggtctgc ttggctactt tggcctggac
    69601 cgcatgttgc cgcatgcgca tccgcaggat ttaatgcaaa aatcggtgca gctgttgcca
    69661 aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg
    69721 gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgca
    69781 ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc ggtttatgcg
    69841 gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact agcagaaatt
    69901 cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt ggtcatggcg
    69961 gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    70021 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    70081 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    70141 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    70201 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    70261 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    70321 agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa tatttaacaa
    70381 tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc atatggtcgc
    70441 ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct gatattaatg
    70501 aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc aaaataaaaa
    70561 ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag cggttttcag
    70621 ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt cacgagcgcc
    70681 atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca
    70741 ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat caaatcaata
    70801 gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa
    70861 acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg
    70921 gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc ttcaatggtc
    70981 acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg cagatcaagt
    71041 tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg gcgaatggtc
    71101 agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc ttcgttttca
    71161 cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc cagtgcattt
    71221 tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa
    71281 cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg catatagcca
    71341 aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc cggactctaa
    71401 ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg
    71461 cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc ctgagaataa
    71521 gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac ataattctgg
    71581 tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta cagggaaact
    71641 acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga atcaatgcct
    71701 aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct
    71761 cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg
    71821 ttacggactc gtgagaaact ggcgctgctt gaacaaagaa tcagcgaact ggaaaatcgc
    71881 agtactgaga tcaaaaagca gccagatcca gagactctcc ccccaacgct gtaaacattt
    71941 gtctttaatg cactgtaaat tatgaattaa tcctctaatt tacagtgttt tctattacat
    72001 tatcgcttaa tcccattcag agtatcatca caaaaatgaa tgattgcaat tgaaaatata
    72061 attgttatgt attctttaag ttttacttaa tcgatat
//
LOCUS       DAJTUM010000006        70293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTUM010000006 DAJTUM010000000
VERSION     DAJTUM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..377
                     /locus_tag="OO471_000456"
     CDS             <1..377
                     /locus_tag="OO471_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6241846.1"
                     /translation="RLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLV
                     TVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQ
                     FAFSTPDSEPTTSVLQGTAQCH"
     gene            388..1479
                     /locus_tag="OO471_000457"
     CDS             388..1479
                     /locus_tag="OO471_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6241847.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1522..2382)
                     /gene="rsmI"
                     /locus_tag="OO471_000458"
     CDS             complement(1522..2382)
                     /gene="rsmI"
                     /locus_tag="OO471_000458"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6241848.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2447..4483
                     /gene="lpoA"
                     /locus_tag="OO471_000459"
     CDS             2447..4483
                     /gene="lpoA"
                     /locus_tag="OO471_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6241849.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4441..4836
                     /locus_tag="OO471_000460"
     CDS             4441..4836
                     /locus_tag="OO471_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6241850.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4856..5446
                     /gene="diaA"
                     /locus_tag="OO471_000461"
     CDS             4856..5446
                     /gene="diaA"
                     /locus_tag="OO471_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6241851.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5456..6031
                     /gene="yraP"
                     /locus_tag="OO471_000462"
     CDS             5456..6031
                     /gene="yraP"
                     /locus_tag="OO471_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6241852.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6145..7185)
                     /locus_tag="OO471_000463"
     CDS             complement(6145..7185)
                     /locus_tag="OO471_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6241853.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVSGLALLF"
     gene            complement(7258..7893)
                     /locus_tag="OO471_000464"
     CDS             complement(7258..7893)
                     /locus_tag="OO471_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6241854.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8021..8539
                     /gene="yhbO"
                     /locus_tag="OO471_000465"
     CDS             8021..8539
                     /gene="yhbO"
                     /locus_tag="OO471_000465"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6241855.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8519..8962)
                     /locus_tag="OO471_000466"
     CDS             complement(8519..8962)
                     /locus_tag="OO471_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6241856.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9013..9315
                     /gene="yhbQ"
                     /locus_tag="OO471_000467"
     CDS             9013..9315
                     /gene="yhbQ"
                     /locus_tag="OO471_000467"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6241857.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9302..9805)
                     /locus_tag="OO471_000468"
     CDS             complement(9302..9805)
                     /locus_tag="OO471_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6241858.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9799..10323)
                     /locus_tag="OO471_000469"
     CDS             complement(9799..10323)
                     /locus_tag="OO471_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6241859.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10532..11527
                     /locus_tag="OO471_000470"
     CDS             10532..11527
                     /locus_tag="OO471_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6241860.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11536..12414
                     /locus_tag="OO471_000471"
     CDS             11536..12414
                     /locus_tag="OO471_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6241861.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12495..13502
                     /locus_tag="OO471_000472"
     CDS             12495..13502
                     /locus_tag="OO471_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6241862.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13620..14864)
                     /gene="mtr"
                     /locus_tag="OO471_000473"
     CDS             complement(13620..14864)
                     /gene="mtr"
                     /locus_tag="OO471_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6241863.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15018..16907)
                     /gene="deaD"
                     /locus_tag="OO471_000474"
     CDS             complement(15018..16907)
                     /gene="deaD"
                     /locus_tag="OO471_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6241864.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16900..16980)
                     /gene="yrbN"
                     /locus_tag="OO471_000475"
     CDS             complement(16900..16980)
                     /gene="yrbN"
                     /locus_tag="OO471_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6241865.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17087..17971)
                     /gene="nlpI"
                     /locus_tag="OO471_000476"
     CDS             complement(17087..17971)
                     /gene="nlpI"
                     /locus_tag="OO471_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6241866.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18080..20215)
                     /gene="pnp"
                     /locus_tag="OO471_000477"
     CDS             complement(18080..20215)
                     /gene="pnp"
                     /locus_tag="OO471_000477"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6241867.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20462..20731)
                     /gene="rpsO"
                     /locus_tag="OO471_000478"
     CDS             complement(20462..20731)
                     /gene="rpsO"
                     /locus_tag="OO471_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6241868.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20880..21824)
                     /gene="truB"
                     /locus_tag="OO471_000479"
     CDS             complement(20880..21824)
                     /gene="truB"
                     /locus_tag="OO471_000479"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6241869.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21824..22225)
                     /gene="rbfA"
                     /locus_tag="OO471_000480"
     CDS             complement(21824..22225)
                     /gene="rbfA"
                     /locus_tag="OO471_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6241870.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22389..25037)
                     /gene="infB"
                     /locus_tag="OO471_000481"
     CDS             complement(22389..25037)
                     /gene="infB"
                     /locus_tag="OO471_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6241871.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25062..26549)
                     /gene="nusA"
                     /locus_tag="OO471_000482"
     CDS             complement(25062..26549)
                     /gene="nusA"
                     /locus_tag="OO471_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6241872.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26577..27029)
                     /gene="rimP"
                     /locus_tag="OO471_000483"
     CDS             complement(26577..27029)
                     /gene="rimP"
                     /locus_tag="OO471_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6241873.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27236..27312)
                     /locus_tag="OO471_000484"
     tRNA            complement(27236..27312)
                     /locus_tag="OO471_000484"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27276..27278),aa:Met,seq:cat)
     gene            27660..29003
                     /gene="argG"
                     /locus_tag="OO471_000485"
     CDS             27660..29003
                     /gene="argG"
                     /locus_tag="OO471_000485"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6241874.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29011..30618)
                     /locus_tag="OO471_000486"
                     /pseudo
     CDS             complement(29011..30618)
                     /locus_tag="OO471_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31096..31182)
                     /locus_tag="OO471_000487"
     tRNA            complement(31096..31182)
                     /locus_tag="OO471_000487"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31146..31148),aa:Leu,seq:gag)
     gene            complement(31197..31529)
                     /gene="secG"
                     /locus_tag="OO471_000488"
     CDS             complement(31197..31529)
                     /gene="secG"
                     /locus_tag="OO471_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6241875.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31757..33094)
                     /gene="glmM"
                     /locus_tag="OO471_000489"
     CDS             complement(31757..33094)
                     /gene="glmM"
                     /locus_tag="OO471_000489"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6241876.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33087..33935)
                     /gene="folP"
                     /locus_tag="OO471_000490"
     CDS             complement(33087..33935)
                     /gene="folP"
                     /locus_tag="OO471_000490"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6241877.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34025..35959)
                     /gene="ftsH"
                     /locus_tag="OO471_000491"
     CDS             complement(34025..35959)
                     /gene="ftsH"
                     /locus_tag="OO471_000491"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6241878.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36059..36688)
                     /gene="rlmE"
                     /locus_tag="OO471_000492"
     CDS             complement(36059..36688)
                     /gene="rlmE"
                     /locus_tag="OO471_000492"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6241879.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36814..37107
                     /gene="yhbY"
                     /locus_tag="OO471_000493"
     CDS             36814..37107
                     /gene="yhbY"
                     /locus_tag="OO471_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6241880.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37263..37739)
                     /gene="greA"
                     /locus_tag="OO471_000494"
     CDS             complement(37263..37739)
                     /gene="greA"
                     /locus_tag="OO471_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6241881.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            37987..39420
                     /gene="dacB"
                     /locus_tag="OO471_000495"
     CDS             37987..39420
                     /gene="dacB"
                     /locus_tag="OO471_000495"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6241882.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39606..40778)
                     /gene="cgtA"
                     /locus_tag="OO471_000496"
     CDS             complement(39606..40778)
                     /gene="cgtA"
                     /locus_tag="OO471_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6241883.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40794..41759)
                     /locus_tag="OO471_000497"
     CDS             complement(40794..41759)
                     /locus_tag="OO471_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6241884.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41886..42143)
                     /gene="rpmA"
                     /locus_tag="OO471_000498"
     CDS             complement(41886..42143)
                     /gene="rpmA"
                     /locus_tag="OO471_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6241885.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42164..42475)
                     /gene="rplU"
                     /locus_tag="OO471_000499"
     CDS             complement(42164..42475)
                     /gene="rplU"
                     /locus_tag="OO471_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6241886.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42733..43704
                     /gene="ispB"
                     /locus_tag="OO471_000500"
     CDS             42733..43704
                     /gene="ispB"
                     /locus_tag="OO471_000500"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6241887.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43931..44209
                     /gene="sfsB"
                     /locus_tag="OO471_000501"
     CDS             43931..44209
                     /gene="sfsB"
                     /locus_tag="OO471_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6241888.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44257..45516)
                     /gene="murA"
                     /locus_tag="OO471_000502"
     CDS             complement(44257..45516)
                     /gene="murA"
                     /locus_tag="OO471_000502"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6241889.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45571..45825)
                     /gene="ibaG"
                     /locus_tag="OO471_000503"
     CDS             complement(45571..45825)
                     /gene="ibaG"
                     /locus_tag="OO471_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6241890.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(45985..46278)
                     /gene="mlaB"
                     /locus_tag="OO471_000504"
     CDS             complement(45985..46278)
                     /gene="mlaB"
                     /locus_tag="OO471_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6241891.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46278..46913)
                     /gene="mlaC"
                     /locus_tag="OO471_000505"
     CDS             complement(46278..46913)
                     /gene="mlaC"
                     /locus_tag="OO471_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6241892.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46932..47483)
                     /gene="mlaD"
                     /locus_tag="OO471_000506"
     CDS             complement(46932..47483)
                     /gene="mlaD"
                     /locus_tag="OO471_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6241893.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47488..48270)
                     /gene="mlaE"
                     /locus_tag="OO471_000507"
     CDS             complement(47488..48270)
                     /gene="mlaE"
                     /locus_tag="OO471_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6241894.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48278..49087)
                     /gene="mlaF"
                     /locus_tag="OO471_000508"
     CDS             complement(48278..49087)
                     /gene="mlaF"
                     /locus_tag="OO471_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6241895.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49297..50262
                     /locus_tag="OO471_000509"
     CDS             49297..50262
                     /locus_tag="OO471_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6241896.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50276..51262
                     /gene="kdsD"
                     /locus_tag="OO471_000510"
     CDS             50276..51262
                     /gene="kdsD"
                     /locus_tag="OO471_000510"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6241897.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51283..51849
                     /gene="kdsC"
                     /locus_tag="OO471_000511"
     CDS             51283..51849
                     /gene="kdsC"
                     /locus_tag="OO471_000511"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6241898.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51846..52421
                     /gene="lptC"
                     /locus_tag="OO471_000512"
     CDS             51846..52421
                     /gene="lptC"
                     /locus_tag="OO471_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6241899.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52390..52947
                     /gene="lptA"
                     /locus_tag="OO471_000513"
     CDS             52390..52947
                     /gene="lptA"
                     /locus_tag="OO471_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6241900.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            52954..53679
                     /gene="lptB"
                     /locus_tag="OO471_000514"
     CDS             52954..53679
                     /gene="lptB"
                     /locus_tag="OO471_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6241901.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53727..55160
                     /gene="rpoN"
                     /locus_tag="OO471_000515"
     CDS             53727..55160
                     /gene="rpoN"
                     /locus_tag="OO471_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6241902.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55183..55470
                     /gene="hpf"
                     /locus_tag="OO471_000516"
     CDS             55183..55470
                     /gene="hpf"
                     /locus_tag="OO471_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6241903.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55588..56079
                     /gene="ptsN"
                     /locus_tag="OO471_000517"
     CDS             55588..56079
                     /gene="ptsN"
                     /locus_tag="OO471_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6241904.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56125..56979
                     /gene="rapZ"
                     /locus_tag="OO471_000518"
     CDS             56125..56979
                     /gene="rapZ"
                     /locus_tag="OO471_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6241905.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            56976..57248
                     /gene="npr"
                     /locus_tag="OO471_000519"
     CDS             56976..57248
                     /gene="npr"
                     /locus_tag="OO471_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6241906.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57461..58093
                     /gene="yrbL"
                     /locus_tag="OO471_000520"
     CDS             57461..58093
                     /gene="yrbL"
                     /locus_tag="OO471_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6241907.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58090..58817)
                     /gene="mtgA"
                     /locus_tag="OO471_000521"
                     /pseudo
     CDS             complement(58090..58817)
                     /gene="mtgA"
                     /locus_tag="OO471_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(58814..59467)
                     /gene="elbB"
                     /locus_tag="OO471_000522"
     CDS             complement(58814..59467)
                     /gene="elbB"
                     /locus_tag="OO471_000522"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6241908.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(59697..62033)
                     /gene="arcB"
                     /locus_tag="OO471_000523"
     CDS             complement(59697..62033)
                     /gene="arcB"
                     /locus_tag="OO471_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6241909.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62129..63058)
                     /locus_tag="OO471_000524"
     CDS             complement(62129..63058)
                     /locus_tag="OO471_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6241910.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63733..68193
                     /gene="gltB"
                     /locus_tag="OO471_000525"
     CDS             63733..68193
                     /gene="gltB"
                     /locus_tag="OO471_000525"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6241911.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68206..69624
                     /gene="gltD"
                     /locus_tag="OO471_000526"
     CDS             68206..69624
                     /gene="gltD"
                     /locus_tag="OO471_000526"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6241912.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            69808..70287
                     /locus_tag="OO471_000527"
     CDS             69808..70287
                     /locus_tag="OO471_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6241913.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17211 a        19066 c        17456 g        16560 t
ORIGIN      
        1 accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgaa caaacaacac
       61 agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt
      121 atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt ggcgctcttg
      181 ccagcaacga tgatacgggg caacaaagta tcgtcgatga gggcggcata ctatatctct
      241 ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag gcagatcaac
      301 aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta ttacaaggca
      361 cagcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac aggacttttg
      421 ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagccga cagcactagc
      481 ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac agataacgtg
      541 acaggacggg agtttacctc tgcaacgcta agtagcacta actggcaata cgcctgttcc
      601 tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccccgtact taccaccact
      661 ggacatcaga caggatatta caaactcaat gacagcctgg atattaaaac cacattacag
      721 gcaaacgaca ttccaggact cacaaccgac caggttgtct ctgttaacac ccgattcaca
      781 cagataaaaa gcagcaccgt atattctgct gcaacccaaa cgggtgtttg ccagggtgac
      841 acgtctcgtt atggacccgt taatattggt gcaaatacca cctttaccct gtatgtcacc
      901 aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat taaaggcgcg
      961 tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt caagttatcg
     1021 attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga tgttattaaa
     1081 gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat gccagacggt
     1141 tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa gattaaaaac
     1201 tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa cctggtcgcc
     1261 aggcgaagaa gttcagacaa tgtgcccgat gtcggtattc gtattgaaaa tctcggcggc
     1321 ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc cgggcatggc
     1381 accgtcaata tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga aacaggaaaa
     1441 tttcagggca cagccaccat taccgtcatg gtgcggtaaa agaagacgtt gcgttttgat
     1501 taacgcaacg cttcggcact gttacccctg ctgctccagc gcatacttat acagcgcatt
     1561 tttcttcacg ccatgaattt ctgcggccag cgccgccgct tttttcagcg gcagttctgc
     1621 ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct gtgctttatg
     1681 accttcgaca atcagcacca tttcgccttt gcgacggttt tcatcttcct ttacccacgc
     1741 cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca gctcacgcgc
     1801 cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc tatctaacag
     1861 acggtgggta gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc
     1921 atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac ggtcagatgg
     1981 taaacccgcg gcgcttaacg cagtgatagc agcacacggt ccgggtagcg gcaccacacg
     2041 aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa ttagcggcgt
     2101 tccggcatcg gaaaccagcg caatgttttg cccctcttgc agctttgcca gcagcgtttc
     2161 ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg
     2221 ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa cggcctgtaa
     2281 tacctctaac gtacgctggg tgatatccgc cagattgccg attggcgtcg gtacaatgta
     2341 aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc
     2401 gatttaatat tgagcattgc gtaaaaaaaa tatcactgga tacattatgg taccctcaac
     2461 attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc tgattttcgc
     2521 cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca cggcgcaggc
     2581 tgattctgcc ttttatcttc agcagatgca gcaaagctct gatgatacca ggatcaactg
     2641 gcaattactc gccattcgtg cactggtgaa agaaggtaaa accgggcagg cggttgagtt
     2701 gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa cactgctggc
     2761 ggcagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc tggcgaaaat
     2821 cacacctgcc gatttagaac aaaaccagca agcgcgttac tggcaggcaa aaatcgatgc
     2881 cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg aaccgctgct
     2941 tggcgcgaaa gaaaaacagc agaatattga tgccacctgg caggcgctct cctccatgac
     3001 tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc aaggctggct
     3061 ggatctgcag cgcgtctggt ttgataaccg taacgatccc gacatgatga aagccgggat
     3121 cgccgactgg cagaaacgtt atccgaacaa tccgggcgcg aaaatgctgc caacgcagtt
     3181 ggttaacgta aaagcgttta aaccagcctc gaccaacaaa atcgccctgc tgttgccgct
     3241 gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag ccgcgaaaaa
     3301 tatcggcact cagccagtgg cggctcaggt agctgccgca cctgccgcag acgtagcaga
     3361 acaacctcag ccgcaaactg cggatagcgt tgccagcccg gcacaagcct cggttagcga
     3421 tctgaccggt gatcagcctg cggcccagcc ggtgcctgta agcgccccgg cgacaagcac
     3481 cgcagcggta agcgcacccg caaatccatc cgcagagctg aaaatctacg atacctcatc
     3541 acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta ttgtggttgg
     3601 tccgttgctg aaaaataacg ttgaagagtt gctgaagagc aacaccccgc tgaacgtact
     3661 ggcactgaac cagccggaga atatcgaaaa ccgcgtcaat atttgttact tcgcgctttc
     3721 accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta aacaagcgcc
     3781 gctggtgctg atcccacgca gtgcattggg cgatcgcgta gccaatgcgt ttgcgcaaga
     3841 gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt
     3901 acgcgcgggt gttaacggcg gttctggtat tgctttaacg ggtagcccga ttactcccag
     3961 agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca cgccaaccga
     4021 tgaccagttc accaataatg gcggtcgtgt cgatgcggtc tacattgtgg caacgccggg
     4081 tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga gcggtgcaac
     4141 gctgtacgcc agttcccgca gcgcacaagg caccgcaggc ccggatttcc gactggagat
     4201 ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctgc cgttaatgca
     4261 gcaggcgctc agcgcggtga acaacgatta ttcgctggct cgcatgtatg cgatgggcgt
     4321 tgatgcctgg tcgttggcaa atcatttctc acaaatgcgc caggttcagg gttttgaaat
     4381 caacggtaat accggaagtc tgacggctaa cccggattgc gtgattaaca ggaagttatc
     4441 atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa acagaccggc
     4501 gatgcgtggg aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg gtttatcgcc
     4561 gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg cctgaccacc
     4621 gtttttgtcg aggtacgcta tcgccgctct gcgctttatg gcggcgcggc agccagtgtg
     4681 acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat
     4741 gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt
     4801 gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca
     4861 agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc
     4921 gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa
     4981 caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag
     5041 catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga
     5101 taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca
     5161 ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag
     5221 ccgcgatatt gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt
     5281 gaccggctat gacggcggcg aacttgcagg tttgttaggg ccacaggatg tggagatccg
     5341 cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct
     5401 gtgcgatctg atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa
     5461 ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc
     5521 tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg
     5581 cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga
     5641 acagattaag aaagaaaccc gtattaatgt cacggcctat cagggcaaag tgctgctggt
     5701 tgggcagtca ccaaatgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga
     5761 cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc
     5821 atctaacgat acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt
     5881 gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt
     5941 gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg
     6001 ggttaccaca gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct
     6061 tgccgcgtct tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc
     6121 gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgctga caatcacacc
     6181 agacactgcc accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag
     6241 cataatcagt gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc
     6301 ggttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat
     6361 gacaaacaag catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc
     6421 accatcggca tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat
     6481 gtaaaccggg atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa
     6541 accgctctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg
     6601 cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg
     6661 aatcgccgca aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa
     6721 caccggattg cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat
     6781 tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt
     6841 tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg
     6901 gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt tccatactgc
     6961 gaggaaatag atcatcgcgt aatccaacgc cgcttgccac ggattagcat ccgcctgcgc
     7021 aaggatagat ttaccgatac tgtgggtttc ggctgcagta aacgctttgc cgtagtaagg
     7081 ttgccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca
     7141 ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa
     7201 taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta
     7261 ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg
     7321 catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc
     7381 tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc
     7441 gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc
     7501 ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat
     7561 agcactgacc accagcatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac
     7621 taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt
     7681 gccgagacaa caaaacacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag
     7741 ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc
     7801 agcaatggcg ttaactttcg gttcattaat caacatccgc agcaggtgac cgcccaccag
     7861 ccccgttgcg cctgtaatca gtacctgact catcttcgtt cctttacaga attgtctgcc
     7921 ttgcgctccc cggctcatgc accagtctta atagaccatg aggtaattat ccccgattgt
     7981 ggaaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt
     8041 tttaatcact gatgaatttg aagattcaga atttacttca cccgcagacg agttccgtaa
     8101 agccggacac gaagtgatta ctattgaaaa acaagcgggt aaaacggtga aaggcaaaaa
     8161 aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga
     8221 tgccctgttg ctaccgggtg gtcattcacc ggattatctg cgtggcgaca accgttttgt
     8281 cacctttacc cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc
     8341 gcagctgctg atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat
     8401 cattattgat gttaaaaatg cgggcgtgga attttacgat caggaagtcg tggttgataa
     8461 agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt
     8521 acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt
     8581 tgtcggtgaa tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg
     8641 caaccggaaa gcgacgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc
     8701 tgcggatctc acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt
     8761 ttaccgttcc ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt
     8821 ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaggca ttagcgcacc
     8881 agagttcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc
     8941 tgatggcgat gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat
     9001 gcgatgcaga cgatgacacc ctggtttctt tacttgatcc gtaccgccga caataagctt
     9061 tataccggga tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg
     9121 gcgaaagcac tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgac
     9181 cgttcgctgg cactacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag
     9241 cgtctggtag cggaaggcgc agggtttgca gaactgttga gcagcctgca aaccccggag
     9301 attaaaagcg attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt
     9361 catccgctaa acgatgtacc aggaaggcgc tttcagtgcc cggccagcgg caacgcagat
     9421 cgtgatgcgc cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca
     9481 ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg
     9541 ccagcccctg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca
     9601 ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt
     9661 catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct
     9721 tcgcttccgc atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa
     9781 tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc
     9841 ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt
     9901 cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacaccc ccagctccgt
     9961 atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc
    10021 cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgtgttctg
    10081 gctgaccacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac
    10141 atgaatactt aaccagcggc cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg
    10201 gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt
    10261 taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa
    10321 cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat
    10381 agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg
    10441 ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg
    10501 cgttttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct
    10561 cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga
    10621 tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc
    10681 ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca cttttgcgca
    10741 tccggacggt tacgcccgtt ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga
    10801 cgcgctgatc ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt acccgcatat
    10861 tgaacgtcat gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca
    10921 tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa
    10981 acagctggca cgggttactc ctgtcccgct ggaagtgttt gctttcggca gcctgtgtat
    11041 tatgtcggaa ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacactgt
    11101 gggtgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc
    11161 ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac
    11221 gctgtgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac
    11281 cagtctcaat accctggaac tgctgccgga gttaatggct gcgaatattg cttcggtgaa
    11341 aattgaaggc cgccagcgta gcccggcgta tgtcagccag gcggcgaaag tctggcgtca
    11401 ggctatcgac cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga
    11461 gacgctcggg tcgatgtccg aaggcacgca aaccaccctt ggcgcgtatc accgtaaatg
    11521 gcagtgagaa gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag
    11581 acgctggaag atttttatca gcaagccgcc gccagcagcg ccgacgtgat ttatcttggt
    11641 gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg actgtctgga gatggcaaaa
    11701 tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc
    11761 tctgaactgg gcgaactgaa acgctatgtt gagaacggtg aatttctgat tgaagccagc
    11821 gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg
    11881 ctgaactgct acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg
    11941 tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag
    12001 ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc
    12061 tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc
    12121 tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt
    12181 gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca
    12241 tccatgcagg gattggttga tgtagtccgc ctgtcaccgc agggcactga cactttcgcg
    12301 atgctcgacg ccttccgcgc taatgaaaat ggcgctgtgc cactgccgtt gacggcaaac
    12361 agcgactgta acggctactg gcggcggctg gcaggactgg agctgcaagc ctaagtaaat
    12421 agctcacttt gttaacaact ttaactattc tttaatgcag tattaaagat taatcggtaa
    12481 caaagtgagc tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca
    12541 tacccgaagg ctcttcagcg cgggaagcgt tctcccactc tctcgatctc gcccgtctgg
    12601 ctgaaaagcg cggctatcat cgctactggc tggcagaaca ccacaatatg accggcattg
    12661 ccagtgctgc aacatcggta ttgattggct atctggcggc gaataccacc acgctgcatc
    12721 tggggtctgg cggcgtgatg ttgcctaacc acttaccgtt ggtcattgcc gaacagttcg
    12781 gcacactcaa tacgctctat ccggggcgaa tcgatttggg gctgggtcgt gctccgggta
    12841 gtgaccaacg gacaatgatg gcgctacgtc gtcatatgag cggcgatatt gataatttcc
    12901 cccgcgatgt cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc
    12961 gcccggtacc aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca
    13021 gtgcacaact ggcggcgcag cttggtctgc catttgcgtt tgcctcacac ttcgcgccgg
    13081 atatgttgtt ccaggcgctg catctttatc gcagtaactt caaaccgtcg gctcggctgg
    13141 aaaaaccata cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg
    13201 aatttctgtt tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc
    13261 aactgccgcc gccgattcaa aatatggatc agttctggtc accgtctgag cagtatgacg
    13321 tgcagcaggc gctgagtatg tcgttggtag gcgataaagc gaaagtgcgt catggtttgc
    13381 agtcgatcct gcgcgaaacc gacgctgatg agattatggt taacgggcag attttcgacc
    13441 accaggcgcg cctgcattcg tttgagctgg cgatggatgt taaggaagag ttgttgggat
    13501 agtgtgtctt aacgcgggaa gccttatccg agctggcaac gctgtcctac gtaggcctga
    13561 taagcgaagc gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat
    13621 tactgataca ccggcagtaa attaaagctc gataaaatat gcaccagtgc gttgccgacg
    13681 ccaaacacca gaatcagcgc aatcatcggc ttgccacccc agacgcggaa tttcgggctg
    13741 ccaaagcgtt tacgcgatgc gcgggccagc agtgccggaa caattgccgc ccagatggta
    13801 gccgctaaac cagcataacc aatggcatac aggaatccgt tcgggaacag cagaccaccc
    13861 acaacaggcg gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc cgagtcgtcg
    13921 aaaccaaaca gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc
    13981 gctaccgcaa agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg
    14041 ccgcttaatg cttgtaccaa cacatcaata ttaccgccct tctcggcgat gccgataaac
    14101 tccggacgag ggatgttgcc cattgtcgcc agcaaccaga tggtatacag cgccagtgcc
    14161 atcagcgtac cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc
    14221 atcaggcttg gcacgttacc gtgataacca aacgacgcca ggcagaacgg cagggtcatc
    14281 agcaaatacg gcgcataaga tgcattgctt tcggcaacgt tgaacaatgt cgcaggctgc
    14341 acatgcccca gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagcaca
    14401 atcgccgtca tgcggctgac ggctttagta ctcaaccaca ccacaaacgc taccagcagt
    14461 gcaaaaccaa aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga
    14521 tgcagaatcg aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca
    14581 atggaaatgc cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca
    14641 aaactcgaac cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc
    14701 atacagaacc aggtaaagat cagcgctgcc attgaccaga aaaaccacgc cccggacatg
    14761 accactggca gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg
    14821 ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg ccatgagggc ttctctccag
    14881 tgaaaaatag tgcgactgcg ttgttatgca ttgcactgta ccagtacatg agtacaaaag
    14941 acagaaaaaa agccccgatg gttaaaatcg gggctgtata tattatttta cagattgtgt
    15001 tcgctgttca gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca
    15061 cgacgcggtg cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacgacca
    15121 ccttcacggc gttcaccgct gaagttacga ccgccttcac gacgctcgcc accgaaacca
    15181 cgaccaccgc cacgacgctc accgccagta tgcggctgtg catcgcccag taactgcatg
    15241 ttcatcggct tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata
    15301 cctttcggca gttcgatggt ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg
    15361 ctgctgatgt cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc aacaccatca
    15421 tcgcggccca cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc
    15481 ggacgatctt cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg accacggtca
    15541 tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggaac aatcagagta
    15601 cgttcaccct gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca
    15661 ccttctgcag tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt
    15721 tccagctgct gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct
    15781 gcgttcggca gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc
    15841 agacgacgct cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg
    15901 gtacgaccga tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaaccacc
    15961 aggctgatac gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat caggatgtcc
    16021 agacgaccgt ctttaaggcg ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg
    16081 ttcagcgcgg cgctattgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg
    16141 tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc
    16201 agtgcttcgt ttttacgcat accccacaca gtccagtagc tctggctgat gtcaggacgg
    16261 gtagtcacgc tggactgaat acgcacttcc tgcggctctt tcataaagcg gcgggtaatg
    16321 cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc
    16381 tgcgccataa tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca
    16441 tccagaacca gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc
    16501 agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg
    16561 tcataacgct ggccgccgta cagggcaacc acatttacgc cgcgcatgtg tttagagaaa
    16621 tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc
    16681 tgtggtgctt tcagctcagg atcaagattc tgcagcagag gtaaagagaa tgctgcagtt
    16741 tttccgctcc ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata
    16801 cactctgcct gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata
    16861 ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc
    16921 ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat
    16981 tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt
    17041 aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg
    17101 attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt
    17161 gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt
    17221 ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct
    17281 cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc
    17341 taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt
    17401 cgaagtgctg tttcaatact tctttagcct gcttctcatc gagcttctgc tcggcgagat
    17461 aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat
    17521 cttgcgctaa cttgtcacga ccgccgtaat ataatgcaat cccgcgattc aagtgcgcgt
    17581 agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat
    17641 tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga
    17701 ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca
    17761 acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa
    17821 ggatttgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg
    17881 cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg
    17941 caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg
    18001 aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag
    18061 cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag
    18121 caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa
    18181 cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa
    18241 cgcgtttgtc agcgatttga gagatgtgta ccagaccttc tttaccgccg ccgatggcaa
    18301 caaatgcgcc aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga
    18361 tttctgcagt gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg
    18421 ctgcaatctt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt tcttcggtca
    18481 gggcacggat aacagagccg cctttaccga taacgtcttt gatcttgtcc gggttgatct
    18541 tgatggtatg gatacgtggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct
    18601 gttccattac gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca
    18661 tgatctcttt ggtgatacct tcaattttga tatccatctg caatgcagag ataccctcgc
    18721 gggaacccgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag
    18781 acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag
    18841 ctttgatcgg cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag
    18901 aagaggaacc gttggattca gtgatttcag acacaacacg tacggtgtac gggaatttgt
    18961 ccatatccgg catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac
    19021 gacgcttcgg agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt
    19081 ggaacaggaa ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag
    19141 cagtacccag cgttgcggta accagcgcct gcgtttcacc acgggtgaac agcgcagaac
    19201 cgtgagtacg cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac
    19261 gaccgtcgat acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatcg
    19321 cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg
    19381 cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc
    19441 ggtaagcatc gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt
    19501 ttaccggctc cggctgccag tcccaacgtg gtttaccggc ttctttcacc agttcattga
    19561 tgttctgaat aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctggt
    19621 cttcgctcag cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa
    19681 caaccagatc cagtttgctc tctttcaact cgtcctgagt cgggttcagt acgtactggt
    19741 cattgatgta acctacgcga gcagcaccaa tcggaccatt gaacggaata ccagacagag
    19801 acagtgctgc ggaagcacca atcatcgcga cgatatccgg gttaacttgc gggttaacag
    19861 aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcggccgaa
    19921 tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac
    19981 ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca
    20041 gtgggaagaa gtcctgacct ggtttggctt ttttctggcc aacaacggta acaaataccg
    20101 cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt
    20161 ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa
    20221 tatcctttct ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc
    20281 gcatcctcgc gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag
    20341 ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa
    20401 ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga
    20461 attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt
    20521 tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt
    20581 ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca
    20641 gtgctacctg aacttcagta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga
    20701 ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa
    20761 ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta
    20821 cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat
    20881 tacgccgggt attcaaccac caggcgacga ggcgcaacgc ggccttcatc gtcaatttcg
    20941 cccataccga taaatttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc
    21001 gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga
    21061 ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac
    21121 tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg
    21181 gtcacaatcc gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc
    21241 gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt
    21301 gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa
    21361 acggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt
    21421 tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg
    21481 aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg
    21541 atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag
    21601 cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc
    21661 aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa
    21721 atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac
    21781 aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt
    21841 cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca
    21901 tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca
    21961 ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga
    22021 tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat
    22081 acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat
    22141 ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct
    22201 gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc
    22261 cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca
    22321 gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc
    22381 taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg
    22441 ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact
    22501 tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg
    22561 ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag
    22621 cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata
    22681 atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc
    22741 aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt
    22801 gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg
    22861 gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg
    22921 tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc
    22981 ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg
    23041 agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt
    23101 gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt
    23161 acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga
    23221 cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg
    23281 cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg
    23341 aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga
    23401 acttccgcct gcagcaggat agcgtccagc agttcatcga taccagtacc cgctttcgca
    23461 gacacatgta cgaactggct ttcaccgccc cactcttccg gcaggatgcc gtactgggag
    23521 agttcgttct taacgcgatc cggatcggct tctggtttat cgatcttgtt cactgcaacg
    23581 accaccggaa cctgcgccgc tttcgcgtgc tggattgctt cgatcgtctg cggcattacg
    23641 ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc acgagcacgc
    23701 attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt
    23761 tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggccactttc
    23821 gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc
    23881 acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct
    23941 tccagctcgt tttcacgacg caggataact ttatggccca tctcttcagc aaccagctgt
    24001 gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct
    24061 ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt
    24121 tcgccgatca caacgtcacg gttaacggcc tgagcaggct tctggaagcc ttgctgcagc
    24181 gaagaacctt tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga
    24241 tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga
    24301 ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt
    24361 tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat
    24421 ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt
    24481 ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg ctcacggcgg
    24541 gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt
    24601 tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca
    24661 cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc
    24721 tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg
    24781 cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg
    24841 cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca
    24901 atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca
    24961 tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta
    25021 atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac
    25081 cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg
    25141 atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg
    25201 gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag atcgtcagcc
    25261 ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta
    25321 gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct
    25381 ttcatcggca cataggccag ttcttccagc gtcgagaaac cttcttctac cagaacagtc
    25441 gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc
    25501 gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt tgagttccca accgctcagc
    25561 tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccagct
    25621 tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc
    25681 ggtgccattg cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata
    25741 cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa
    25801 gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc
    25861 gggtcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttccgg cacttcaata
    25921 cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc
    25981 gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg gaagttttca
    26041 cgcggcagca tatcttcgcg caggatcacg gcttcagcgt tgttgcccag atccagagag
    26101 atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg
    26161 aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt
    26221 gccgtctggg tggtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg
    26281 cccaagttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc
    26341 tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga
    26401 tcgatctgta cacggacgtc gatctcttgc tcatattttt tctttgttgc tgtcgccagc
    26461 gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct
    26521 tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa
    26581 aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct
    26641 tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga
    26701 cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag
    26761 tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca
    26821 acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa
    26881 tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg
    26941 cgaataaatt cgatgcctac cagttcaaaa cccagggcct caactggcgc agtaatcatc
    27001 tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta
    27061 aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa
    27121 ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc
    27181 cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt
    27241 gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg
    27301 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac
    27361 tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg
    27421 ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt
    27481 gttgaatact tttgccttcc cctgctctcc cttaagcgca ttattttaca aaaaacacac
    27541 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
    27601 aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta
    27661 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg
    27721 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat
    27781 atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
    27841 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
    27901 gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca
    27961 acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag
    28021 atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct
    28081 atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
    28141 actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt
    28201 tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa
    28261 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta
    28321 tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct
    28381 ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
    28441 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaga
    28501 accgtatcat cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc
    28561 acattgcgta tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc
    28621 acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg
    28681 cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
    28741 ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga
    28801 cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
    28861 gtattggtca attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg
    28921 gttatgccaa aactggcctg ctttcctcct ctgccacttc aggcctgccg caggtggaga
    28981 atatggaaaa caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
    29041 ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
    29101 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg
    29161 ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa
    29221 gatattgttt tccgttgtgc gatccacgcc atccccaaga acggggctat accagataaa
    29281 catcgggaca tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt
    29341 cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
    29401 ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg agttgtcata
    29461 gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat aggcgctaca
    29521 agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtattttg
    29581 ctgtaatgct tgcgataaat gcggcaacaa caattcatca aatcctctga cataaactgt
    29641 ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa aagcggattg
    29701 cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa tgttgtcggg
    29761 gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag
    29821 cgcagtgtaa ggtgcgccgc ttattgcttg attaaacagt ttgatctgtt ttctttgtgc
    29881 ttcaacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt cgacacgtac
    29941 agactccccc acaatcaaca cgtaggtatc aatacttgta tccctgactg ataattgaaa
    30001 atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc gctttcgctg ctaaagcaaa
    30061 atagttgaga ttgaaaaacg gcgtataggt agcaaatcac gacgctagta tatatggact
    30121 gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa ataaactgcc
    30181 cgaaatgaca atcagcaata atattcctgt cacttttttt gtcggcaagg aaacatcata
    30241 ttttattatt actgccaaaa aaagaaggga taaaaaagca aaagcacata gataagggat
    30301 atacatcccc agcattttca caacttcatc cggatcactc tgcagcacac taatcgcaaa
    30361 tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac tcatgtctgc
    30421 cgcagtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg atgatatacg
    30481 tttaacaacc aggatggtca ggacgaagaa cagcaatcgc tgaaccatat gcgctccgga
    30541 agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca accaatgaga
    30601 tttaaaatct tctagcaaat ttgttgaata ctgtcatcca gaaatccaag ttcacaacca
    30661 atataaacaa ttatcaacaa tgttcaattt tatatcagac atgtttttca actctgatcc
    30721 atatcagacc gaaacactgt tagttttttc tttttaaatg atgcgaccat tgcgttctca
    30781 gtaattaaat aacattatca atgcgtctct aaacattacg cagaacgtag ccatcagtag
    30841 ttatactgac aggagcagga tggtttatcg agaagtaaac atatccgtat tcgtaccgga
    30901 actggagttg acacaataaa gtgccaatta tgtcagcagt agggaaaagt ttataactaa
    30961 ggcgtaccgg caccatcgtt tcaaggtacc agctacgagt aaagcaactg gacgagatac
    31021 agatacctga caaccattca tcagaccagg accaaaacga aaagagacgc ttttcagcgt
    31081 ctcttttctg gaatttggta ccgaggacgg gacttgaacc cgtaagccct attgggcact
    31141 accacctcaa ggtagcgtgt ctaccaattc caccacctcg gcacggatac tactttttag
    31201 ttcgggatat cgctggtcgg cttagccgga gcagctggct gagtttgttc ggttttcgcc
    31261 ggtgcactca gattttccca ttcgctacct ctattggttt tgttgctatt gatgttaccc
    31321 agcaccagac tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag
    31381 ttaccagaac cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc
    31441 atatcagcgc ctttaccttg ctgcagcatg atcagaccaa caaggccaat tgccacaata
    31501 aggaaaacta ctaaaagagc ttcgtacata atcaacctgt tccttgcgga gttgccgcgt
    31561 accaatgctt caaccaataa agcgggagtt tttaatcttt cccactgaag cgggtgtgaa
    31621 tactaaccaa aacgaatgac cttcgcaagg gcaattttat cgcattgtat caactgcgga
    31681 aaaaaacagc aaaaccgatg tgttcgccga gaaaaaaggc gacaatcgcc gcctttttag
    31741 ccagttatct aacgctttaa acggctttta ctgcatcggc gatgcggtgt gcaaattcag
    31801 tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat taacggttcg gtgccggatt
    31861 tacgcagcaa cacgcgtcca cggttgccca gcgcagcttc aacctctgcg gtcacggctt
    31921 taactgactc atgctcaagc ggatcgccgc tacctgcggt gtaacgtacg ttaaccagaa
    31981 tctgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag gctcatatgg ttacgtgcca
    32041 tcgccgccag cacctgcaag ccagcaacga tgccgtcacc ggtagtggtt ttatccagca
    32101 ggatcacatg accggaattc tctgcaccga tacgccagcc tttctcctgc attttttcca
    32161 gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca
    32221 gttcaagccc catgttgctc atcaatgtac ccacagcacc accacgcagc tggccctgac
    32281 gaagaccttc acgcgcgatg atatacatga tctgatcgcc atcgacttta ttgccttcat
    32341 ggtcaaccat aatcacgcga tcgccgtcgc cgtcgaaggc aataccgaga tccgcttttt
    32401 cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt
    32461 ttacaccgtt tggctcacaa ccgatagcga taacgttcgc ccccagttcg cgcagcacgt
    32521 tcggcgcgat gtgataagtc gcaccgtttg cacaatccac cacaatcttc agttcactga
    32581 ggctaagttc gttcgggaac gtggctttgc aaaactcgat atagcgaccc gcggcatcaa
    32641 cgatacggct ggctttaccc agttcagccg aatcaacgca gctgatctcc ttttccattt
    32701 ccgcttcgat ggcctcttct accgcatccg gcagtttagt gccgtcgata gagaagaatt
    32761 taatcccatt atcgtagaac gggttatgcg atgcagatat cacaattccg gcctctgcgc
    32821 ggaaggtacg cgtcagataa gccacggccg gtgttggcat cgggccagtg aagagtgcgg
    32881 aaaggcccgc tgccgccaga cccgcttcca gtgctgactc cagcatatag ccagaaatac
    32941 gcgtgtcttt accaataata atcttacggg agccgtggcg cgccagcact ttacccgcgg
    33001 cccaacccag cttaagcaca aaatcaggtg tgatcggcgc atcccctaca cgaccacgaa
    33061 tcccatcggt accgaaatat ttacgattac tcatagcgtt tgttttcctt tgcagacaga
    33121 gtggcttcca ccacccgcat cgcttctacg gtttctttga cgtcatgaac acgaatgatg
    33181 tgcgcgcctt gcattgcggc aatgaccgca caggccagac taccgctcag gcgctcggac
    33241 ggccccacgt tcagcagctg cccaatcatc gattttcgtg acatacccac caacagcggc
    33301 aggttgaaat ggtgaaattc agccaggcgc gccagtaatg aatagttatg ggagagattt
    33361 ttaccgaaac cgaatccggg gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg
    33421 caacgtgcta tttgctcaat aaagtagcga ttcacttctg caaagacatc gtcatacttc
    33481 ggagcttcct gcatggtttt tggatttccc tgcatatgca tcagacaaac cggtaaaccg
    33541 gtttctgcag ccgcctccag agcgccaggt tcggaaaggg agcggatatc attaataatg
    33601 tgagcgccaa ctttcgctga ctcacggatg acttctggtt tggatgtatc gaccgagatc
    33661 cagacttcga agcgttgagc aattgcctca accacaggaa taacacgttg caactcttct
    33721 tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc caccaacgtc aatgatcgtc
    33781 gcgccagcat tgatcatcag attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg
    33841 ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg aggatggcta
    33901 aggtccagtg aagtaccctg ggcaaagagt ttcattatgt tatccctggt atgaattgat
    33961 aagaaaaaac cccggagcac gccccggggt tttcggtaca aatacagtca tctgatgcgg
    34021 gaacttactt gtcgcctaac tgctctgaca tggtgttacc cgggttcggc gtacgcggtt
    34081 catcaaccgg acgaggagcc tttggactac cattgtcgcc agaattgtta gaagcgcctg
    34141 gttcttccca gcccgctggc ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg
    34201 gtgcgtcgat agtctcatat ttcatgagag catctttcat cgcatgcaga atatccatat
    34261 tgtcggtcag aagctgacgc gcacgattat agttacgctc aatcagtgct ttcacttcct
    34321 ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg ctacggccga
    34381 ggaatacttc accttcttct tccgcgtaca gcagtggacc caatttctca gagaagcccc
    34441 actgagtcac catgttacgt gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg
    34501 tagaaacatg ttccggcccg tagatgatct cttctgccag acgaccaccg tacagcgtag
    34561 aaatctggct ttccagtttc tgacggctgg cgctgattgc gtcgccctca ggcaagaaga
    34621 aagtcacacc cagcgcacga ccgcgtggga taatcgtcac tttatgcacc ggatcgtgtt
    34681 ccggcaccag gcgaccgata atcgcatgac ccgcttcgtg gtaagccgtt gattctttct
    34741 gcgcttccgt catcaccatg gagcgacgtt ccgcacccat catgattttg tctttcgctt
    34801 tctcgaactc aaccatcgac acaacgcgtt tgttgccacg agcagcgaac agtgccgctt
    34861 cgttcaccag gttcgccagg tcagcaccgg agaaaccagg agtaccacga gcaatgattg
    34921 ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg aactttcagg atctgctcac
    34981 gaccgcgaac atctggcaag ccgaccacaa cctggcggtc gaaacggcca ggacgcagca
    35041 gggccgggtc gagaacgtcc ggacggttag tcgcggcgat aacgatgata ccttcgttac
    35101 cttcgaagcc atccatctca accagcatct ggttcagagt ctgttcacgt tcatcgtgac
    35161 caccgcccag accagcgcca cgctggcggc ctacggcgtc gatttcatcg ataaagatga
    35221 tgcacggtgc cgctttcttc gcctgttcga acatgtcacg aacacgggat gcacccacac
    35281 cgacgaacat ttctacgaag tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc
    35341 ctgcaatcgc tttcgccagc aacgttttac cggtacccgg aggaccgacc atcaggacgc
    35401 ctttcgggat cttaccgccg agtttctgga agcggctcgg ctcgcgcaga tactctacca
    35461 gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga
    35521 tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa cgacatggca cctttgccac
    35581 cgccgccctg catttgacgc atgaagaaga tccagacacc aatcagcaac agcatcggga
    35641 accaggagat gaagatagaa gccagcaggc ttggttcttc aggcggttca ccgacaacct
    35701 tgacgttctt ggtcaacagg ttatccagta atttcggatc ctgaaccgga atgtaagtgg
    35761 tataacggtt actatctttc ttggtaacgt tgatttcacg tccgttgata cgcgcttcac
    35821 gaacctggtc gttattgacc tcttgtagga aggtagagta atccacctta cggccattag
    35881 actcgctggg cccaaagctc tggaatactg acatcagcac aacggcaatg accagccaga
    35941 gtattaggtt tttcgccatg tcactcaagg gattaacctc ttattacaac tgtgttaaaa
    36001 acagcgtcag gatactctat atccagcatc tttcaaactt tcgtctgaaa tctcccggtt
    36061 agggtttacg cccggtcgct acaatataca cttctcgcga acgtgcacga gaagagtccg
    36121 gcttacgaac tttgaccttc gtaaacaggg agcgaatttc ccttagatac tcatcgaaac
    36181 cttcgccctg gaacaccttc actacaaaac tgccacctgg cgctaataca tcacgacaca
    36241 tttctagcgc cagttccacc agatacatgg ctcgggggat atccaccgcc ggtgttccgc
    36301 tcatgtttgg tgccatatcg gacatgacaa cctggacttt gctgtcgcca acgcgctcca
    36361 gcagtgcttt catcaccagt tcatcacgaa aatcgccctg aagaaagtcc acaccaacga
    36421 taggatccat aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca atttgggtga
    36481 ccacatattg tgaccaacca cccggagcag caccaaggtc gacaaccgtc attcccggtt
    36541 taaagagttt gtcactttgc tgtatttcat caagtttaaa ccaggcacgg gaacgtaatc
    36601 cctttttctg tgcctgttga acatatttat cgctaaagtg ttcctgaagc cagcggctgg
    36661 agctggcaga acgcttctta cctgtcattt aactttccca tggggataac tcatcgtaac
    36721 caattgcgta aatttttacg cgcctatttg gtgatatatg ggagatggcg gtagaatgac
    36781 ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc tgagtactaa acaaaaacag
    36841 cacctgaaag gtctggcaca tccgctcaag ccagttgttc tgcttggcag taatggtttg
    36901 accgaagggg tgctggccga gattgaacaa gcgttagagc accatgaact catcaaggtg
    36961 aaaatcgcca ccgaagatcg cgaaacaaaa accttgatcg tggaagctat cgtgcgcgaa
    37021 accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa
    37081 gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag ttacacacat ttgctgtgta
    37141 aaacgagggg ttttccgcag gcaggagagc aaaatgccac gctctgttcg ttgataaaag
    37201 gccgcagagc ggcctttttc ctttctttac aatacatcaa catcttgagt attgggtaat
    37261 tcttacaggt attccacctt aattacttca aattctactt cgccgcccgg cgttttgatg
    37321 accacaacat catcttcttc tttgccgatc aggccacgag caataggcga gtttacagaa
    37381 atcaggtttt gtttaaagtc agcttcgtca tcgccaacga tgcgataagt ctgttcttcg
    37441 tcagaatcca gattcagcac cgttacggta gcaccaaaaa taacgcgccc attgttgggc
    37501 attttggtga catcaatcac ctgcgcgttt gacagcttgg cttcgatgtc tttgatacgg
    37561 ccttcgcaga agccctgctg ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg
    37621 ccatgctcac gcgcttccgc gatagcagca atgatttcag gacggcgcac agatttcaga
    37681 aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt
    37741 tgttatacct cttgaatatt cctgatgggg caagtcttca cccaccccgg ctgttcggcc
    37801 tgcccggcat aactcctcac cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc
    37861 ccaggtcagc tacaattcac attttgatgg tcattttacc ctgaagttcc cgaagggtca
    37921 tcgtttactt tatagggcgt tgcgccgtag tatgacggct cgattccagg ttgttagcgc
    37981 gagattatgc gattttccag atttatcatc ggattgacca gctgtatagc gttcagtgtt
    38041 caggccgcga atgttgatga gtacgttact caactccccg caggtgccaa ccttgccctg
    38101 atggtgcaaa aagtcggtgc gtcggccccc gctattgatt accacagtca gcagatggcg
    38161 ctgcccgcca gtacccagaa agtgattact gcgctggcgg cgttgattca actcggcccc
    38221 gattttcgtt tcaccacgac gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag
    38281 ggtgacttag tggcgcgatt tggtgccgat ccgacgttaa aacgtcagga tattcgcaat
    38341 atggtcgcga ctttgaaaaa atctggcgtc aaccaaatcg atggcaatgt gttgatagat
    38401 acctccattt tcgccagcca cgataaagcc cccggctggc catggaatga catgacacaa
    38461 tgctttagcg ctccgcctgc tgccgccata gttgaccgca actgtttctc cgtctcgctc
    38521 tacagtgccc caaagcctgg tgatatggct tttatacgcg tggcatctta ttaccccgtt
    38581 acgatgttca gccaggtacg caccctcccc cgtggttctg ccgaagcgca atactgcgaa
    38641 ctggatgtgg tgccaagcga cctgaaccgc tttacgctga cgggatgcct gccacaacgt
    38701 tctgagccgc tcccgttggc ttttgccgtg caggatggag ccagctatgc cggtgcaatt
    38761 ctgaaagatg agttaaaaca ggcgggtatc acctggagcg gaacactgct gcgccagact
    38821 caggttaacg aacctggaac ggtagttgcc agtaaacagt cggccccgct gcacgatctg
    38881 cttaagatta tgctgaaaaa gtcggacaac atgatcgccg atacggtttt ccgcatgatc
    38941 ggccatgcgc gcttcaatgt gcctggaaca tggcgggctg ggtcggacgc cgtgcgtcag
    39001 atcctgcgcc agcaagccgg tgtcgatatt ggtaacacca ttattgccga tggttcaggg
    39061 ctttcgcggc ataacctgat tgcccccgcc accatgatgc aggtgctgca atacattgcc
    39121 caacacgaca atgaacttaa ctttatctcc atgctgccgc ttgcgggcta tgacggctct
    39181 ttgcagtacc gtgcaggtct gcatcaggcg ggcgtggatg gcaaagtctc agcgaaaacc
    39241 ggttcgttgc agggggtata taacctggcg ggattcatta ccacagcgag cgggcaacga
    39301 atggcatttg tacaatatct ttctggctat gcagtaaaac ccgcggatca gcgtaatcgc
    39361 cgtattccgt tagtgcgttt tgaaagccgt ttgtacaaag atatttatca gaacaattag
    39421 tcaaaaagaa accccggcgc atggccgggg tttcagatta ttgacaaagt gcgctttgtt
    39481 tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcgtgcaaa ttcaatatat
    39541 tgcaatcatc ttgtaggcct gataagtgta gcgcatcagg cttatttggc attggtcatt
    39601 agtgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca
    39661 tcccagtctt catcatcctc ttcagcaatc tcttcaagct gctgacggtg ataatcatcc
    39721 cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca
    39781 atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccgct cgccgcagag
    39841 atcagataat atttatcttc ccagcccagc gcttcagcga tcgctttcgc tttctcttcg
    39901 gcttctgcct tatccagcag atcgatcttg ttgaagacta accaacgcgg tttcgccgcc
    39961 agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg
    40021 gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc
    40081 ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata
    40141 tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga
    40201 gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc
    40261 gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc
    40321 agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact
    40381 gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc
    40441 tgaccgtgtt tagtcatatc acccatggtt tcaccagtac cctggtcgat tacacgcgta
    40501 cctaccggca ctttaatcgt cacgtcttta ccgcgcttac cggtacaatc gcggcttgcg
    40561 ccgttctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc
    40621 aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg
    40681 ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct
    40741 gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg
    40801 cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga
    40861 ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg
    40921 tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac
    40981 agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc
    41041 atcgcttccg ccaaggcgcc atatcctacc aaggtattca gtccgcaaaa aattaaacat
    41101 gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgcg
    41161 attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa
    41221 accttttgcg ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact
    41281 ccccaggtgt aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc
    41341 accagaccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc
    41401 aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa
    41461 gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg
    41521 gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc
    41581 aacctcttct tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa
    41641 aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc
    41701 ccccagcaaa ttgctgtggt aagcgccaaa agaatgccaa tgcctgcctg ctgcttcata
    41761 ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt
    41821 tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga
    41881 aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat
    41941 ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg
    42001 ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg
    42061 ccaccgaaac gcttaacgcc caagcgttta gcttctgaat cgcgaccgtt acgtgtggag
    42121 ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag
    42181 taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac
    42241 gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga
    42301 ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca
    42361 gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg
    42421 tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa
    42481 actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag
    42541 ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa
    42601 aaaaacacaa cttgtacggt aacgtttatc tgtgccattt tttcagtaca attaccctat
    42661 attcctaacc ataaacccta agttgccttt gttcacagta aggtaatcgg ggcgaaaagc
    42721 ccggcttttg cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga tatggcgggt
    42781 gttaatgcgg caatccttga gcagcttaat tccgacgtcc aactgatcaa tcagttaggc
    42841 tattacatcg tcagcggcgg cggtaaacgt attcgtccga tgattgctgt actggctgca
    42901 cgagctgttg gctatgaggg aaatgcgcat gtcaccattg ctgccctgat cgagtttatc
    42961 cacacggcga ctctgctaca cgacgacgtt gtggatgaat cagatatgcg caggggtaaa
    43021 gctaccgcca acgccgcatt tggcaatgcc gccagcgtgc tggtaggcga ttttatttat
    43081 acccgcgcgt tccagatgat gaccagcctc ggttcactca aagtgctgga agtcatgtca
    43141 gaagctgtaa acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt taacgatccg
    43201 gacatcactg aagaaaacta catgcgcgtt atctatagca aaaccgcgcg tctgtttgag
    43261 gctgccgcgc agtgttccgg gattctggct ggctgtacgc cggaggagga gaaaggcctg
    43321 caggattatg ggcgctatct cggcactgct ttccagttga tcgacgattt actcgattac
    43381 aatgccgatg gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga aggtaaaccg
    43441 acgctgccgc tgctgcatgc gatgcatcat ggcacaccag aacaggcaca gatgatccgt
    43501 accgccatcg aacaaggtaa cggtcgccat cttctggaac cggttctgga agcaatgaac
    43561 gcttgtggat ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga caaagccatc
    43621 gcagcgttac aggtgctccc ggacacccct tggcgagaag cactcatcgg cctcgcgcac
    43681 atcgctgttc aacgcgatcg ttaatcccct cccttcatcc cgcgcagtgc gcgggatgat
    43741 tccagtaaat tccataaaaa cactaattca gcgctataac acctgaaaaa agggcgatga
    43801 atctttatat attctgaata ttcacactct ttacaggaac tttttagcgc aatagaccat
    43861 caggagtata gtgatgcccg acagaagaag tgttctgaat gaaagcgact aacttaagga
    43921 gtgaggaaag atggaaagta atttcattga ctggcatccc gctgacatca ttgcaggttt
    43981 gcgcaagaaa ggaacgtcaa tggcggcgga atctcgcaga aatggtttga gttcctcaac
    44041 gctggcaaac gcattatcgc gcccatggcc gaaaggagag atgattattg cgaaagccct
    44101 gggaactgac ccctgggtta tctggccatc acgctaccat gatccgcaga cccatgagtt
    44161 tatcgacaga acgcagttga tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg
    44221 gtagccccgc gaacggggct gccagctctc agacgattac tcgcctttca cacgctcaat
    44281 atttgcacct aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat cgatgtgata
    44341 aatacgatca accaccgtcg tcccttccgc aatacagcca gccagcacca ggcttgctga
    44401 tgcacgcaga tcggttgcca taacctgtgc accagaaagt ttttcaacac catgacaaat
    44461 aacggtattg ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg gcacatgcat
    44521 aaagcggttt tcaaagaccg tttcggtgat gaacccggtc ccttcagcca ccaggttcaa
    44581 cagcgtgaac tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt
    44641 aacagccttc ggacgtttgc catgcatatc caggctaatc cagtcttcgc cgacttcgat
    44701 gtccgctcca gcatcacgca gtttcgccag cacggcgtcc agagtatctg gctgcgcgtt
    44761 acggcagata attttgccac gagagatcgc cgccgccacc aggaaagtac cggtttcgat
    44821 acgatccggc agcacgcgat agacaccgcc gcctaaacgt tccacacctt cgatgacgat
    44881 acgatcggtg ccctgaccgc taattttcgc acccagcgta atcaggaagt tcgcggtatc
    44941 gacgatttcc ggttcacgcg ctgcgttttc aataatcgtg gtgccttccg ccagggttgc
    45001 agcacacatg atggtcaccg ttgcgccaac gctgacttta tccatcacga tatgtgcgcc
    45061 tttcagacga ccatcgacgg aagctttaac gtaaccttct tccagtttga tggtcgcgcc
    45121 taattgttcg aggccagaaa tgtgtagatc aaccggacgc gcaccgatcg tacaaccgcc
    45181 aggtagtgaa acttgcccct gaccaaagcg cgctaccagc ggccccagcg cccagataga
    45241 agcacgcatg gttttaacca gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc
    45301 aatatgcaca gaaccattac gttctacttt cgcacccagc tggcttagca gtttcattga
    45361 tgtatcgacg tctttcagtt tcgggacgtt ctggatctct accggttctt ccgccagtag
    45421 tgcggcaaaa agaataggca gagcagcatt tttagcgccg gaaattgtga cttcgccctg
    45481 gagcttcgtt ggcccctgaa cacgaaattt atccatttag tttgttctca gttaacaatt
    45541 catatccgct accggcgaat cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc
    45601 ccactccgca ggggtatacg ctttgatcga cacagcatga atgcggttat ccgcaatata
    45661 ttccatcagc gggccataga ccgtctgctg ttttttaacc cgactcatac cgtcaaacaa
    45721 ctcacccacg gcaataacct gaaagtggct gccatcgccg gaaacgtgga cttcctggag
    45781 ggagagagcg ttcatcaaca cgctctgaat ttcattattt tccatgggat cttcaatcat
    45841 cagttaataa accagcgaaa catcttagag caaagttgcg ctggcataaa taagcaaaaa
    45901 gcctcgctga taaatcagac aaggctcgac ttgcaggcag gtttgccgga caggcggtta
    45961 acgccatatc cggcctgaaa aaatttaacg aggcagaaca tcagcaggca aattatacaa
    46021 tttcgcgagg gtatacactt tgtcgtttac cccctgaagc gtcacgttgt tgccctgctt
    46081 tttcgccaga tcgataagat ggagcagcag tgccagtccc cccgtatcca cgcgggacac
    46141 acggctaaga tcgatgcagg taatcccctt caccgcctcc tcacgcattt cccaaagcgg
    46201 tagcagaacg tcctgatcca gctctccgga taacgccagc gtgtcacccg tctgcatcca
    46261 gctcagtgac tcgctcatta ttttttctct tccagagtga ttttctgttg agaaatcgat
    46321 ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc ccactcgttt
    46381 tgtttggtgg tgatcatact gacgccttca gcaatcatgt cgtaagcttg ccagtttccc
    46441 gtctgggagt ttttacgcca ctggaagtcc agacgcaccg gcggacggcc attcgggtca
    46501 ataatggtaa cgcgaatagg cacaatggtt ttatcgccca gcggctgttc tggcgcaatc
    46561 tgataggttt gaccgtgata catcgccagc gcctgaccgt aagcctgctt caggtactca
    46621 cggaaagcgg caaagtaggc ttcacgttga gcaggggtcg cactcttgta atactggccc
    46681 agcaccagcg caccggcgta tttcacctgt acgtatggca gcagttcctg atcaacaatg
    46741 gtacgcagat aatccgggtt ggcccgaatt tgcggttgct cattcttcag gcgatcgaac
    46801 gttttctgcg ccgcctcgtc catcagctta taaggattgg tctggtctgc cgcggttgcc
    46861 gcactcagag gtgcaatcac cagcaaagcg accatcatta aacgtttaaa catgcgtcgg
    46921 ttctcctgaa attatttcgt tgtacccaca ggttcagtgg tttcattatt acctggcgca
    46981 gcagctggcg catcgccact attcttattg tcatcgcctt tactaccgta aaggaactga
    47041 ccaatgagat cttccagcac catcgcagac ttggtgtcct gaattgtatc gccatccttc
    47101 aggatagcag tccccagttc cgggtcttca aaaccgacgt ttaatgccag atattgttct
    47161 cccagcaggc cggaagtacg aatgctcagc gaactggtat caggaatgtg gttataacgt
    47221 tgttcaattt ccagcgttac gcgcggcaga taggttttcg ggtccagcgt aatatccgcc
    47281 acccggccca caacaacacc accaatgctg accggagagc gcgctttcag gccgccaatg
    47341 ttatcgaacg tcgcataaag cgtgtaggtc gattcagtac gtatggacgt cacgttcgcc
    47401 gccttcaggc aaacaaacag cgccgccagc agtgctgcta ataaaaagat acccacccaa
    47461 atttcatttt ttttcgtttg catgaactca attcccaaac atcaatgcgg tcagcacaaa
    47521 atccagcccc agaacagcca gagacgagtg gacaacggtg cgagtggttg cccggctaat
    47581 cccggcagac gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat
    47641 ggcgaacacc acgctcttaa tcagacagtt gaccagatcc atacgccagt cgacggcatt
    47701 ttgcattgcc gaccagaaga acccgctatc aatgcctttc caactgacgc cgaccagcga
    47761 tccgccccag atccccacgg cgacgaagat aaccgtcaac agtggtaatg aaataacccc
    47821 agcccagaaa cggggagaaa taacccgacg cagcggatcc accgccatca tctccatact
    47881 ggagagttgc tctgtagcgc gcatcaggcc gatttctgcg gttaacgccg aaccagcacg
    47941 cccggcaaac aacagcgcgg caaccaccgg ccccagttca cgcagtagcg ataacgccac
    48001 cagcataccc agactggttt ccgcgctata agtggtcaga accagataac cttgcagccc
    48061 caacaccatt ccgatgaaca cgccagaaac tacaataatc agcatcgaca ggacgccgac
    48121 attatagagc tggcgcacca gcagcggcgc atgtttgcga aattccggtt tgccgaccag
    48181 cgcattgaat aacattaacc cggcccgccc gaacgttctc agggttttaa tccctttatg
    48241 tccgagcgac gccagcgcat ttaacagcat gagtggctta actccctggt aaaagatcag
    48301 cgtgataatc gccggcagga tagcggaacg gaacaggccc gtcagctatc ccgtccagaa
    48361 actgacgtac gcgcggatca ggattcgtct gcaacgcctg ggcactgcca tgagcgacaa
    48421 tttttttgtc cgccaggatc caggcgtgat ccgcaatact taacacttcc ggcacatcgt
    48481 gagaaaccac cacacaagtc acgcccagcg cgctgttcag ctcagaaatc agcttcacca
    48541 gtacgcccat ggtgatggga tcttgcccaa caaaaggttc atcaaacatg atgagatccg
    48601 gctccagcgc aatcgcacgc gccagcgctg cacgccgcgc catcccaccg gaaagttcag
    48661 aaggcattag tttagccgct ccacgcagcc ccacggcctc cagcttcatc atcaccgtac
    48721 tatgcaacaa tggcgcggga agttgggtat gttcgcgcag tggataggcg acgttgtcaa
    48781 atacgttcat atcagtgaac aacgccccgg actgaaataa catgctcatc cgtttacgca
    48841 ctgtatacag gcgcgaacga gacatcgccg gaatattctc accatcgaaa aggatctcac
    48901 catgatctgg tgcgatttgc ccgccaatca gacggagtag cgtcgtttta ccgatgcccg
    48961 atggccccat gatcgccgtg atcttccctc gcggcacggt cagggaaata ttatcgaaga
    49021 tgcagcgatt gccacgcgta aaactgacat cgcgcatatc gactaaattc gccacagact
    49081 gctccataat tcacccttcg tcttgcgttg attttctaag catggcgctc aatttaacct
    49141 tgaacccaac atatttacag aatattaccc gccgtggtta gcgaaagctg gcatttgttt
    49201 tactttttag ccgcataaag tcaaaattaa gaatccgtta cggctttctg aaaatcttca
    49261 gcggaccggc gagtatacct gaagaaagga cgttagatgc ttttagctac ggcactgtta
    49321 attgttggtt tacttttggt cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt
    49381 ctttgccgaa cctttggcat cccgccgctg atcatcggca tgacggtggt cagtattggt
    49441 acatcgttac cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta
    49501 gccgttggta cagccctcgg ctcaaacatt atcaatatat tgctgatcct cggtctggct
    49561 gcgctggttc gtccttttac cgtccattcc gatgttctgc gccgtgaatt acccttaatg
    49621 ttgttggtca gcgtggtggc cggttccgta ctctatgacg gacaacttag tcgaagcgat
    49681 ggtatctttc tcctctttct ggctgtgcta tggctgctgt tcattgttaa acttgcacgt
    49741 caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga cggcggattg
    49801 cccgtcgcgt ttttatggct cggcattgcg cttatcatca tgccagtggc cacgcggatg
    49861 gtggttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt gacgatggct
    49921 ctgacggcaa ttgctatcgg aaccagtctg ccggaactgg caaccgcaat agcgggggtt
    49981 cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg gcgcaaacat ttttaatatt
    50041 gtcatcgtgt tgggtttacc cgcgctgata acgccaggag agattgatcc actggcgtac
    50101 agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct gtgctggcgg
    50161 cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt tatcgtatgg
    50221 ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac gcattatgtc
    50281 gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag tcctggcgat
    50341 tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca cgcttgcctg
    50401 tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga aatcggggca
    50461 catcgggcgc aaaatggccg caacgtttgc cagcaccggt acaccttcat ttttcgtcca
    50521 tcctagtgaa gccgcgcatg gtgatttagg catggtcacc ccacaggatg tggtgattgc
    50581 tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc ttaagcgtct
    50641 tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc gcgccgcaga
    50701 tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg caccgaccag
    50761 cagcaccacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg
    50821 cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact
    50881 tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg ttaagaaaac
    50941 ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa aatcttggta tgactgtcat
    51001 ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt
    51061 cgatatgggc gtggatgttc gtcagttaag tattgccgat gtgatgacgc cggggggaat
    51121 acgtgtgcgc cctggcattc tggccgttga ggcactgaac ttaatgcagt cccgccatat
    51181 cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata tgcatgattt
    51241 actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa agcaggtgcg
    51301 tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc agagaacatt
    51361 cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg gcctgattta tatgggcaat
    51421 aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc
    51481 acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt agaagatcgt
    51541 tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact gatcgccttt
    51601 agcgatttgc tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt tggcgatgat
    51661 ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat
    51721 ccactgttga tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg
    51781 cgcgaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc caaagggcaa
    51841 tcgatatgag taaagccaga cgttgggtta tcattgtgct atcactggcg gttctggtga
    51901 tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg
    51961 atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa ggggcactaa
    52021 gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt tcgtggttta
    52081 cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc gtaaaagcag
    52141 ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt gaagtcaacg
    52201 cacttgtgcc agactctcaa cttcgcagaa tcacgacgga taacgcgcag atcaatctgg
    52261 tgacgcagga tgttacctct gaagacctcg tcacgttata cggaacaaca tttaactcca
    52321 gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt gaaaaggtta
    52381 gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct tgccagctca
    52441 cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca gccgatccac
    52501 attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt taccggtaat
    52561 gtcatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt tacccgtccg
    52621 ggcggcgaac aaggtaaaga agtgattgac ggatacggta aaccggcaac gttctaccag
    52681 atgcaggaca acggtaaacc cgttgaaggt cacgcttccc agatgcacta cgaactggca
    52741 aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag caacattaag
    52801 ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag cgacaaaggc
    52861 aagcgcgtaa ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag
    52921 accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc aaagaacctt
    52981 gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt caactccggg
    53041 gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt ctacatggtt
    53101 gtaggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga tatcagtctg
    53161 ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccacagga agcctccatt
    53221 ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg tgacgacttg
    53281 tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca cattgagcac
    53341 ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc
    53401 cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc cggggttgac
    53461 ccgatctcgg ttatcgacat taaacgcatc attgaacacc tgcgcgacag cggcctgggc
    53521 gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc
    53581 gtcagtcagg ggcatttgat cgcccacggc acgcctacag aaatcttaca agacgaacac
    53641 gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag tttgcgacgt
    53701 tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg cttagccaac
    53761 aactggcgat gacgccacag ctccaacagg caattcgtct gttgcagttg tcgacgctgg
    53821 aacttcagca ggagctacag caggcgctgg agagtaatcc gctgcttgag caaatcgaca
    53881 ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga aacgctggac accgccgacg
    53941 cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg gacaccattt
    54001 acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgacgacgag ctgccggtct
    54061 accagggcga aacgacgcag accttgcagg attacctgat gtggcaggtc gagctgacac
    54121 cgttttccga cactgaccgc gctattgcta cctctatcgt cgatgccgtt gatgaaaccg
    54181 gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa gagatcgaca
    54241 tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt tgacccggtc ggtgtagcgg
    54301 caaaagatct gcgtgactgc ctgctaatcc aactctccca attcgataag accacgccgt
    54361 ggctggaaga ggccagattg atcattagcg atcatctcga tctgttagcc aatcacgact
    54421 tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc
    54481 tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa cctgagtatg
    54541 tcattccaga tgtgctggtg cgtaagcata acggtcactg gacggtagaa ctcaacagtg
    54601 acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat aacgcgcgca
    54661 acgatggtga cagccagttt atccgcagca atctgcagga tgccaaatgg ttgatcaaga
    54721 gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc
    54781 aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc gatatcgccc
    54841 aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata
    54901 gtccacgagg catttttgaa ctgaagtatt tcttttccag tcacgtcaat accgagggcg
    54961 gcggcgaagc ttcctccacg gcgattcgtg cactggtgaa gaaattaatc gcggcggaaa
    55021 acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa caaggtatca
    55081 tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg ccgtcaaacc
    55141 agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct caacattacc
    55201 ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttacagctaa atttgccaaa
    55261 cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc
    55321 acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc cagcgcggaa
    55381 ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg ccagttgacc
    55441 aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg tgcatggcgg
    55501 cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgagcctg ttccactgtt
    55561 tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct acagcttagc
    55621 agtgttctta acagggaatg tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc
    55681 ctggaaatca tcagcgagct ggcggcgaaa caacttagcc tgccgcctca ggtggttttt
    55741 gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg tattgccatt
    55801 ccgcatggca aacttgaaga agatactctg cgtgccgttg gcgttttcgt tcagctcgaa
    55861 acgcctatag ctttcgatgc catcgacaac caaccggtgg acttactttt tgccctgctg
    55921 gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc gaaacgtctg
    55981 gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa
    56041 atcattacgg ataccgaagg tactccggat gaagcgtagt tattcggtaa tgtctctttt
    56101 agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg ttcaggttca
    56161 ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt ggataacctt
    56221 cccgtagtgt tgttacccga tctggctcga actctggccg atcgcgagat ttctgccgcc
    56281 gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca ggcgatgagt
    56341 aacctgcctg acgctttctc accgcaacta ctgttcctgg atgccgaccg taatacctta
    56401 attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg
    56461 gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg agcggatctg
    56521 attgtcgata cctcagaaat gtccgttcac gagctggctg aaatgctgcg tacccgtctg
    56581 ctgggtaaac gtgaacgcga actgaccatg gtctttgagt ctttcggctt caaacacggt
    56641 atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc gcactgggat
    56701 ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac
    56761 acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg gttacctatg
    56821 ctggaaacca acaaccgtag ctacctgacg gtcgccattg gttgtaccgg cgggaagcac
    56881 cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg taaaaacgtc
    56941 cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa
    57001 tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg
    57061 gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa gccaacagcg
    57121 ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt gaagcgaccg
    57181 gtccacagga agaggaagca ctggccgccg ttatcgccct ctttaattct ggttttgatg
    57241 aagattaatc ttcatcactt tgacatacaa ctcccttcaa gctcccccga caataagaaa
    57301 atcacgtact gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg cttttcactt
    57361 tcagaagaac cttaagaaaa ccttaagagg cattgtttag gttttgttta agttaatcga
    57421 ccatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct tatctgaaca
    57481 aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag atgcccaacg
    57541 ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc gccgtgagtt
    57601 aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac cgcgctatca
    57661 cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg ccgattttga
    57721 cggcaagcct tccattacgc tgaccgaatt tgctgaacaa tgccgttacg aagaagacat
    57781 tgcacaactg cgccagctac tgaaacagct gaagcgttat ttgcaggata accgtatcgt
    57841 gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt cagaggttat
    57901 cccggtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga cctggtcgaa
    57961 gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc aaccggcgct
    58021 ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc
    58081 gcgcgaagct taatccagct ggtgttgttg cataaacggc tcaccgccta actgatacat
    58141 ctgccgtaaa atccacgcct gacggctacg cacgtagccc gatggcgagg agactttgaa
    58201 acgaagtgga ttaggtaata cagctgccag taatgcagct tccgaccggg taagtttgct
    58261 cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa
    58321 ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg tttctatccc
    58381 cagcgttaat ccagcttcca gcccttttcg tacccagcta cgcccatccc ataaaaagag
    58441 attttggccg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg
    58501 ttgtgcgcca gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc
    58561 tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc
    58621 cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg
    58681 accattaccg ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc cccgccccag
    58741 aaaacggcga ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt
    58801 aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc
    58861 tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt
    58921 cttcatcaac cacgatatca tcgacaggac acggcacatg ctccgcgccc atctcttcca
    58981 gcacttctgc ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt
    59041 tcggcagcat cgccggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg
    59101 cttgtgccag cgcctttaat tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat
    59161 tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc agttcagcgg
    59221 catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca
    59281 cattgcgcgt ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct
    59341 tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca
    59401 ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt
    59461 tcttcattgt gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat
    59521 ggtacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa
    59581 ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg
    59641 cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat
    59701 tttttagtgg cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct
    59761 ttcatctctt caatccattc accgacgtta tcttcccagg cgggaaggtc aggagactga
    59821 atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt
    59881 ttatgtcctt cctcaacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg
    59941 ctgacatagc ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt
    60001 cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg
    60061 ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc
    60121 atcgcggtta gcgccggaac cgaaagcggc ttactcagca catcatccat tccagcattg
    60181 aggtactctt gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc
    60241 tcgcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat atctggcaac
    60301 tgaatatcca gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg
    60361 ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg cgcgacaatc
    60421 acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct
    60481 tcatcaaacg catcatcgac ctcttctgcc accgacggtg cgtggatcgt caacgtaaag
    60541 gttgaacctt tgccctgttc actggtaacc gtaatatcgc cgcccatatt tttcgccaga
    60601 cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct
    60661 ttcacctggt aatacatggc gaagatttta tccagctcat cctgcggaat gccgatacca
    60721 gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc
    60781 tggccttgct gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa
    60841 cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg taggctccag gttaaagcgc
    60901 aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg
    60961 aaatcaaccg gctgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata
    61021 atatcgttaa agatattccc cagcgtgacg gccgaaacat ggatagtttt gagatatttt
    61081 tcctgctcgg cggtgagttc ggtatccagc agaatgcggc tcaggccgac gataccgttc
    61141 agcggtgtac gcaattcgtg actgatggtg gaaataaacg tcgttttgtc gcggctggcg
    61201 cgttcaagcg catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa
    61261 ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg
    61321 cgcccgtccg ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact
    61381 ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc
    61441 aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg
    61501 gaaaactctt tatcttcgtt acgataaaaa accaagtcgg gtgaagcatc aaggaaggaa
    61561 cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca
    61621 attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcaatt
    61681 tcctgattta gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg
    61741 cgcatctcct ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc
    61801 tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata
    61861 gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt
    61921 accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct
    61981 aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa
    62041 ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg
    62101 tgagaaatgc ggaattcccc tcaacgacct actccgttgg aggtagccac ggacgtccca
    62161 gcgccgatcc ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc
    62221 atcgattttc gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc
    62281 ggtggtagat cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtataat
    62341 cctccagttc aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt
    62401 tcacaatatg cagcggatgc agctttatgc cgtccacgct ggtttcaacc actcgctcca
    62461 gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca
    62521 ccttcagccc gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc
    62581 cgcggttgat gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca
    62641 cttcgtaacc ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt
    62701 ccgggcgggt accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca
    62761 tagaacgcag cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat
    62821 agcgtttagc gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat
    62881 gctgctgcgc ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga
    62941 tggtaccgtc acggttaggg cagctaaaac cgccgtgcag cgtcagctta tgcacctttt
    63001 gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa
    63061 tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga
    63121 tgacctggat caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg
    63181 aaatttcatg cacaatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct
    63241 ccttatggat tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata
    63301 aaatgctggt tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt
    63361 caggaattga ctgtttctct aacgactctc cttttagcct taaagataaa atctatttta
    63421 atttcagcta tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc
    63481 catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga
    63541 gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat
    63601 gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc
    63661 cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg
    63721 gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg
    63781 atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg
    63841 gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc
    63901 ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt
    63961 ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga actcgccgct
    64021 gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg
    64081 cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc
    64141 attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cacgcgatat ggagcgtcgt
    64201 ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc
    64261 tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg
    64321 cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct gttccaccag
    64381 cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg ctatctggcg
    64441 cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat
    64501 aaattccaga cgccgctgat ccctgacctg cacgacgccg cgccattcgt caacgaaacc
    64561 ggttctgact ccagttccat ggataacatg ctggaactgc tgctggcagg cgggatggat
    64621 atcatccgcg ccatgcgcct gttagtgcca cccgcctggc agaacaaccc ggatatggac
    64681 ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggtccg
    64741 gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta acctcgaccg taacggtctg
    64801 cgtccggcac gctacgtcat taccaaagac aagctcatca cctgcgcctc tgaagtcggt
    64861 atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg
    64921 atggtgatcg acactcgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg
    64981 aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt
    65041 gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc
    65101 taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc
    65161 gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc
    65221 agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg
    65281 ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa
    65341 atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt
    65401 ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat
    65461 acgctggata tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagcta
    65521 tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct ctccgaccgg
    65581 aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc gatccagacc
    65641 cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc
    65701 cgcgatccgc accacttcgc cgtgttgctg ggtttcggcg cgacggctat ttatccgtac
    65761 cttgcctatg aaacgctggg ccgcctggta gacacccacg cgattgccaa agattatcgt
    65821 accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa
    65881 atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta
    65941 cacgatgatg tagtgggcct gtgcttccag ggcgcggtca gccgcattgg tggggcaagc
    66001 tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct ggcgcgcaag
    66061 ccgattagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcctacaac
    66121 ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag
    66181 gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt
    66241 acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa
    66301 cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg
    66361 gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg
    66421 gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc
    66481 gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg
    66541 aagccgggcg aaggtggtca gttgccgggc gacaaagtca ctccttacat cgccaaactg
    66601 cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct
    66661 atcgaagact tagcgcagct tattttcgac ctcaagcagg ttaacccgaa agcgatgatc
    66721 tccgttaagc tggtgtccga accaggcgtt ggcaccatcg cgactggcgt ggcgaaagct
    66781 tatgcggact tgatcaccat cgcgggctat gacggcggca ccggcgcaag cccgctgtcg
    66841 tcagtgaaat atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca acaggcgctg
    66901 gttgccaacg gcttgcgtca taaaattcgt ttgcaggttg atggcggcct gaaaacgggt
    66961 gtcgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggccca
    67021 atggtggcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg cgccacgggt
    67081 gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc gttcaaggtg
    67141 actaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca gctgggagta
    67201 acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc
    67261 gccaaacagc agaaactggc gctgtcgaag ctgctggaga ctgccgagcc acatgcaggt
    67321 aagtcactct actgcactga aaacaacccg ccgtttgata acggcctact gaacgcgcag
    67381 ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat
    67441 atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac
    67501 ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag
    67561 agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac
    67621 tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc
    67681 ttccgcagcc atgaagcaag cattatcggc aacacctgcc tgtatggcgc gaccggtggt
    67741 cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc
    67801 accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt
    67861 attctgggta aaaccggcgt taacttcggc gcgggcatga ccggcggctt cgcttacgtt
    67921 ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactggtcga agtcttaagc
    67981 gtcgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag
    68041 cataccggtt ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccacgaaa
    68101 ttcgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt
    68161 agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt
    68221 tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgtaaa
    68281 attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac
    68341 cgctgtctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc
    68401 ccaaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcaga actgtcgcat
    68461 cagaccaaca cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg tctgtgcgaa
    68521 ggttcctgca ccctgaacga tgagttcggt gcggtgacca tcggcaacat tgagcgttat
    68581 atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc
    68641 ggtaaaaaag tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg
    68701 acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg
    68761 accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc
    68821 ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga cgtacagctg
    68881 gacgatctgc tgagtgatta cgatgccgtg ttccttggcg ttgggactta tcagtcaatg
    68941 cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc
    69001 gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg
    69061 gaaggcaaac gcgtggtagt acttggcggt ggcgacactg cgatggactg cgtgcgcacg
    69121 tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg
    69181 ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac
    69241 gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggta
    69301 cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt
    69361 tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg tccacacaac
    69421 atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgctccg
    69481 gaaggcaacg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc
    69541 gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc ggcagacggc
    69601 attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg
    69661 gcgctgaaat tctcctttct ttctggaaat cgcctcgcat aatctgaaga aaaccaatgt
    69721 aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca
    69781 acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc
    69841 taccagcaaa tccacaacgg cattattcat ctggtcgata gcgcccgaac ggaaacggta
    69901 cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa ttggccgacg gattgtcgaa
    69961 tttgaacaag gtggtgaggc cagagcagct tatggtgcac agttgattga tcggctatcg
    70021 aaggatttaa gtcgacggta taaacgtggg gtctctgcaa aaaacttacg ccaaatgagg
    70081 cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg cgaattaaca
    70141 ccattgggaa ttccgcagac accatctgca gaattcccat cggcaaagat ttggcagacg
    70201 ctgtctgcca aattgagagg ctgtttgata tttccacagc ttttgtattg catctcctat
    70261 tttcggtttg tatattcatg caaataaacg aag
//
LOCUS       DAJTUM010000007        67671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTUM010000007 DAJTUM010000000
VERSION     DAJTUM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..228
                     /locus_tag="OO471_000528"
     CDS             <1..228
                     /locus_tag="OO471_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6241914.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO471_000529"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO471_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO471_000530"
     CDS             1365..1688
                     /locus_tag="OO471_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6241915.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO471_000531"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO471_000531"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6241916.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO471_000532"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO471_000532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6241917.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO471_000533"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO471_000533"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6241918.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO471_000534"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO471_000534"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6241919.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO471_000535"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO471_000535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6241920.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO471_000536"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO471_000536"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6241921.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO471_000537"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO471_000537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6241922.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO471_000538"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO471_000538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6241923.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO471_000539"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO471_000539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6241924.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO471_000540"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO471_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6241925.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO471_000541"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO471_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6241926.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO471_000542"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO471_000542"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6241927.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO471_000543"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO471_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6241928.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO471_000544"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO471_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6241929.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO471_000545"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO471_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6241930.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO471_000546"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO471_000546"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6241931.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO471_000547"
     CDS             complement(21226..23058)
                     /locus_tag="OO471_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6241932.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO471_000548"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO471_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6241933.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO471_000549"
     CDS             complement(23806..24300)
                     /locus_tag="OO471_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6241934.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO471_000550"
     CDS             complement(24383..24838)
                     /locus_tag="OO471_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6241935.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO471_000551"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO471_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6241936.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO471_000552"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO471_000552"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6241937.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO471_000553"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO471_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6241938.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO471_000554"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO471_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6241939.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO471_000555"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO471_000555"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6241940.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO471_000556"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO471_000556"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6241941.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO471_000557"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO471_000557"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6241942.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO471_000558"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO471_000558"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6241943.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO471_000559"
     CDS             33414..34307
                     /locus_tag="OO471_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6241944.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQIRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO471_000560"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO471_000560"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6241945.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO471_000561"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO471_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6241946.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO471_000562"
     CDS             complement(36223..36939)
                     /locus_tag="OO471_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6241947.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO471_000563"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO471_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6241948.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO471_000564"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO471_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6241949.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO471_000565"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO471_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6241950.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO471_000566"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO471_000566"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6241951.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO471_000567"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO471_000567"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6241952.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO471_000568"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO471_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6241953.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO471_000569"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO471_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6241954.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO471_000570"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO471_000570"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6241955.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO471_000571"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO471_000571"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6241956.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO471_000572"
     CDS             complement(45788..46447)
                     /locus_tag="OO471_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6241957.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO471_000573"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO471_000573"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6241958.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO471_000574"
     CDS             complement(47342..48355)
                     /locus_tag="OO471_000574"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6241959.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO471_000575"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO471_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6241960.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO471_000576"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO471_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6241961.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO471_000577"
     CDS             complement(50648..51826)
                     /locus_tag="OO471_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6241962.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO471_000578"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO471_000578"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6241963.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO471_000579"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO471_000579"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6241964.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO471_000580"
     CDS             complement(55608..55886)
                     /locus_tag="OO471_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6241965.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO471_000581"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO471_000581"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6241966.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO471_000582"
     CDS             complement(56468..57277)
                     /locus_tag="OO471_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6241967.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO471_000583"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO471_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6241968.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO471_000584"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO471_000584"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6241969.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO471_000585"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO471_000585"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6241970.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO471_000586"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO471_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6241971.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO471_000587"
     CDS             complement(60978..61868)
                     /locus_tag="OO471_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6241972.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO471_000588"
     CDS             complement(61855..62379)
                     /locus_tag="OO471_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6241973.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO471_000589"
     CDS             complement(62376..62846)
                     /locus_tag="OO471_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6241974.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO471_000590"
     CDS             complement(62843..63391)
                     /locus_tag="OO471_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6241975.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO471_000591"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO471_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO471_000592"
     CDS             complement(64138..64860)
                     /locus_tag="OO471_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6241976.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO471_000593"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO471_000593"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO471_000594"
     CDS             complement(66023..66778)
                     /locus_tag="OO471_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6241977.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO471_000595"
     CDS             complement(66860..67423)
                     /locus_tag="OO471_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6241978.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16922 a        18284 c        16783 g        15682 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa attttccttt ttttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg gtcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaggaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagt ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aatccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtac gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg a
//
LOCUS       DAJTUM010000008        66983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTUM010000008 DAJTUM010000000
VERSION     DAJTUM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO471_000596"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO471_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6241979.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO471_000597"
     CDS             complement(1151..1237)
                     /locus_tag="OO471_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6241980.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO471_000598"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO471_000598"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6241981.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO471_000599"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO471_000599"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6241982.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO471_000600"
     CDS             3586..3630
                     /locus_tag="OO471_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6241983.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO471_000601"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO471_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6241984.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO471_000602"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO471_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6241985.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO471_000603"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO471_000603"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6241986.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO471_000604"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO471_000604"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6241987.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO471_000605"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO471_000605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6241988.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO471_000606"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO471_000606"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6241989.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO471_000607"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO471_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6241990.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO471_000608"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO471_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6241991.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO471_000609"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO471_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6241992.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO471_000610"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO471_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6241993.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO471_000611"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO471_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6241994.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO471_000612"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO471_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6241995.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO471_000613"
     CDS             complement(16981..17745)
                     /locus_tag="OO471_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6241996.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO471_000614"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO471_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6241997.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO471_000615"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO471_000615"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6241998.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO471_000616"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO471_000616"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6241999.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO471_000617"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO471_000617"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6242000.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO471_000618"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO471_000618"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6242001.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO471_000619"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO471_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6242002.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO471_000620"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO471_000620"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6242003.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO471_000621"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO471_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6242004.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO471_000622"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO471_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6242005.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO471_000623"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO471_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6242006.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO471_000624"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO471_000624"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6242007.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO471_000625"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO471_000625"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6242008.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO471_000626"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO471_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6242009.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO471_000627"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO471_000627"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6242010.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO471_000628"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO471_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6242011.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO471_000629"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO471_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6242012.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO471_000630"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO471_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6242013.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO471_000631"
     CDS             complement(40421..41815)
                     /locus_tag="OO471_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6242014.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO471_000632"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO471_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6242015.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO471_000633"
     CDS             complement(42158..43444)
                     /locus_tag="OO471_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6242016.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO471_000634"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO471_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO471_000635"
     CDS             complement(44451..45221)
                     /locus_tag="OO471_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6242017.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO471_000636"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO471_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6242018.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO471_000637"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO471_000637"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6242019.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO471_000638"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO471_000638"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6242020.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO471_000639"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO471_000639"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6242021.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO471_000640"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO471_000640"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6242022.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO471_000641"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO471_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6242023.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO471_000642"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO471_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6242024.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO471_000643"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO471_000643"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6242025.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIIEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO471_000644"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO471_000644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6242026.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO471_000645"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO471_000645"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6242027.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO471_000646"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO471_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6242028.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO471_000647"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO471_000647"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6242029.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO471_000648"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO471_000648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6242030.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO471_000649"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO471_000649"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6242031.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO471_000650"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO471_000650"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6242032.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO471_000651"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO471_000651"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6242033.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="OO471_000652"
     CDS             complement(66071..66289)
                     /locus_tag="OO471_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6242034.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="OO471_000653"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="OO471_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6242035.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16248 a        18239 c        17245 g        15251 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacgct tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgag gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccatga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac acatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctctatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg actaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt
    66901 gaggtattaa acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt
    66961 cataaatatc tcctcattag acc
//
LOCUS       DAJTUM010000009        66630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTUM010000009 DAJTUM010000000
VERSION     DAJTUM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO471_000654"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO471_000654"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6242036.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO471_000655"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO471_000655"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6242037.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO471_000656"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO471_000656"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6242038.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO471_000657"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO471_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6242039.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO471_000658"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO471_000658"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6242040.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO471_000659"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO471_000659"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6242041.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO471_000660"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO471_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6242042.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO471_000661"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO471_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6242043.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO471_000662"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO471_000662"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6242044.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO471_000663"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO471_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6242045.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO471_000664"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO471_000664"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6242046.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO471_000665"
     CDS             complement(15665..16702)
                     /locus_tag="OO471_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6242047.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO471_000666"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO471_000666"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6242048.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19312)
                     /gene="ggt"
                     /locus_tag="OO471_000667"
     CDS             complement(17582..19312)
                     /gene="ggt"
                     /locus_tag="OO471_000667"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6242049.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSRIITTVLQMVVNSIDYGMNVAEATNAP
                     RFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDP
                     RSVDDLTAGY"
     gene            19432..19872
                     /locus_tag="OO471_000668"
     CDS             19432..19872
                     /locus_tag="OO471_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6242050.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19859..20602)
                     /gene="ugpQ"
                     /locus_tag="OO471_000669"
     CDS             complement(19859..20602)
                     /gene="ugpQ"
                     /locus_tag="OO471_000669"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6242051.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20599..21589)
                     /locus_tag="OO471_000670"
                     /pseudo
     CDS             complement(20599..21589)
                     /locus_tag="OO471_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21591..22436)
                     /gene="ugpE"
                     /locus_tag="OO471_000671"
     CDS             complement(21591..22436)
                     /gene="ugpE"
                     /locus_tag="OO471_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6242052.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22433..23320)
                     /gene="ugpA"
                     /locus_tag="OO471_000672"
     CDS             complement(22433..23320)
                     /gene="ugpA"
                     /locus_tag="OO471_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6242053.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23418..24734)
                     /gene="ugpB"
                     /locus_tag="OO471_000673"
     CDS             complement(23418..24734)
                     /gene="ugpB"
                     /locus_tag="OO471_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6242054.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25131..25844)
                     /gene="livF"
                     /locus_tag="OO471_000674"
     CDS             complement(25131..25844)
                     /gene="livF"
                     /locus_tag="OO471_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6242055.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25846..26613)
                     /gene="livG"
                     /locus_tag="OO471_000675"
     CDS             complement(25846..26613)
                     /gene="livG"
                     /locus_tag="OO471_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6242056.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26610..27887)
                     /gene="livM"
                     /locus_tag="OO471_000676"
     CDS             complement(26610..27887)
                     /gene="livM"
                     /locus_tag="OO471_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6242057.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27884..28810)
                     /gene="livH"
                     /locus_tag="OO471_000677"
     CDS             complement(27884..28810)
                     /gene="livH"
                     /locus_tag="OO471_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6242058.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28858..29967)
                     /gene="livK"
                     /locus_tag="OO471_000678"
     CDS             complement(28858..29967)
                     /gene="livK"
                     /locus_tag="OO471_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6242059.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30391..30774
                     /gene="panM"
                     /locus_tag="OO471_000679"
     CDS             30391..30774
                     /gene="panM"
                     /locus_tag="OO471_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6242060.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30973..32076)
                     /gene="livJ"
                     /locus_tag="OO471_000680"
     CDS             complement(30973..32076)
                     /gene="livJ"
                     /locus_tag="OO471_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6242061.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32347..33201)
                     /gene="rpoH"
                     /locus_tag="OO471_000681"
     CDS             complement(32347..33201)
                     /gene="rpoH"
                     /locus_tag="OO471_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6242062.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33446..34504)
                     /gene="ftsX"
                     /locus_tag="OO471_000682"
     CDS             complement(33446..34504)
                     /gene="ftsX"
                     /locus_tag="OO471_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6242063.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34497..35165)
                     /gene="ftsE"
                     /locus_tag="OO471_000683"
     CDS             complement(34497..35165)
                     /gene="ftsE"
                     /locus_tag="OO471_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6242064.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35168..36664)
                     /gene="ftsY"
                     /locus_tag="OO471_000684"
     CDS             complement(35168..36664)
                     /gene="ftsY"
                     /locus_tag="OO471_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6242065.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36814..37410
                     /gene="rsmD"
                     /locus_tag="OO471_000685"
     CDS             36814..37410
                     /gene="rsmD"
                     /locus_tag="OO471_000685"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6242066.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37400..37669
                     /locus_tag="OO471_000686"
     CDS             37400..37669
                     /locus_tag="OO471_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6242067.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37672..38031)
                     /locus_tag="OO471_000687"
     CDS             complement(37672..38031)
                     /locus_tag="OO471_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6242068.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38172..38798
                     /locus_tag="OO471_000688"
     CDS             38172..38798
                     /locus_tag="OO471_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6242069.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38872..41070
                     /gene="zntA"
                     /locus_tag="OO471_000689"
     CDS             38872..41070
                     /gene="zntA"
                     /locus_tag="OO471_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6242070.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41172..41417)
                     /gene="tusA"
                     /locus_tag="OO471_000690"
     CDS             complement(41172..41417)
                     /gene="tusA"
                     /locus_tag="OO471_000690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6242071.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41638..42303
                     /locus_tag="OO471_000691"
     CDS             41638..42303
                     /locus_tag="OO471_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6242072.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42376..42933
                     /gene="dcrB"
                     /locus_tag="OO471_000692"
     CDS             42376..42933
                     /gene="dcrB"
                     /locus_tag="OO471_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6242073.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42937..44154)
                     /locus_tag="OO471_000693"
     CDS             complement(42937..44154)
                     /locus_tag="OO471_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6242074.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44288..45337
                     /locus_tag="OO471_000694"
     CDS             44288..45337
                     /locus_tag="OO471_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6242075.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45392..45979
                     /gene="acpT"
                     /locus_tag="OO471_000695"
     CDS             45392..45979
                     /gene="acpT"
                     /locus_tag="OO471_000695"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6242076.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46090..47664
                     /gene="nikA"
                     /locus_tag="OO471_000696"
     CDS             46090..47664
                     /gene="nikA"
                     /locus_tag="OO471_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6242077.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47664..48608
                     /gene="nikB"
                     /locus_tag="OO471_000697"
     CDS             47664..48608
                     /gene="nikB"
                     /locus_tag="OO471_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6242078.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48605..49438
                     /gene="nikC"
                     /locus_tag="OO471_000698"
     CDS             48605..49438
                     /gene="nikC"
                     /locus_tag="OO471_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6242079.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49438..50202
                     /gene="nikD"
                     /locus_tag="OO471_000699"
     CDS             49438..50202
                     /gene="nikD"
                     /locus_tag="OO471_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6242080.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50199..51005
                     /gene="nikE"
                     /locus_tag="OO471_000700"
     CDS             50199..51005
                     /gene="nikE"
                     /locus_tag="OO471_000700"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6242081.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51011..51412
                     /gene="nikR"
                     /locus_tag="OO471_000701"
     CDS             51011..51412
                     /gene="nikR"
                     /locus_tag="OO471_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6242082.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51532..51891)
                     /locus_tag="OO471_000702"
     CDS             complement(51532..51891)
                     /locus_tag="OO471_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6242083.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51888..52163)
                     /locus_tag="OO471_000703"
     CDS             complement(51888..52163)
                     /locus_tag="OO471_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6242084.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52222..53346)
                     /locus_tag="OO471_000704"
     CDS             complement(52222..53346)
                     /locus_tag="OO471_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6242085.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53346..56081)
                     /gene="rbbA"
                     /locus_tag="OO471_000705"
     CDS             complement(53346..56081)
                     /gene="rbbA"
                     /locus_tag="OO471_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6242086.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56078..57145)
                     /locus_tag="OO471_000706"
     CDS             complement(56078..57145)
                     /locus_tag="OO471_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6242087.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57511..59133)
                     /locus_tag="OO471_000707"
     CDS             complement(57511..59133)
                     /locus_tag="OO471_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6242088.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59395..60984)
                     /locus_tag="OO471_000708"
     CDS             complement(59395..60984)
                     /locus_tag="OO471_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6242089.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61369..62400
                     /locus_tag="OO471_000709"
                     /pseudo
     CDS             61369..62400
                     /locus_tag="OO471_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62487..62963)
                     /locus_tag="OO471_000710"
     CDS             complement(62487..62963)
                     /locus_tag="OO471_000710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242090.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62956..63720)
                     /locus_tag="OO471_000711"
     CDS             complement(62956..63720)
                     /locus_tag="OO471_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6242091.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63966..64727
                     /locus_tag="OO471_000712"
     CDS             63966..64727
                     /locus_tag="OO471_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6242092.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64834..65334)
                     /locus_tag="OO471_000713"
     CDS             complement(64834..65334)
                     /locus_tag="OO471_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242093.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65485..>66630)
                     /locus_tag="OO471_000714"
     CDS             complement(65485..>66630)
                     /locus_tag="OO471_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6242094.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16186 a        17885 c        16347 g        16212 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccggtaa
    17881 ccagccaggt tttaccgtct ttcaccacaa tggtcggcga catcgacgac agcgggcgtt
    17941 tgttcggccc gacggcgttg gcatcaccgc ccaccagccc gtaaacgttc ggtacgcccg
    18001 gtttggcgga gaaatcatcc atctggttat taagcagaat accgctctca cccgcgacaa
    18061 tacccgtacc gaaggtggtg ttcagcgtat aggtcaccgc caccgcgtta ccgtctttat
    18121 ccactacaga gtaatgggta gtttgattac tctcataagg cgcaagcttg ccagggcgaa
    18181 tctcactgga cggcttcgct tttttgatat caatttgatc ggcaatggat ttggcatagg
    18241 ctttattggt cagtgcctgc cacgggactt tgacaaaatc cgggtcgcca agatattccg
    18301 agcggtcggc gtaggcgtat ttctccgctt ctgccatgat ttgcatcgca tcggcgctgc
    18361 caaagccgta tttcttcata tcgaagtttt ccagaatatt gaggatttgt acgatatgga
    18421 tcccgccgga ggatggcggt ggcatggagt aaacctgata cccgcgataa tcgccgctta
    18481 tcggagtgcg ttcgaccgct ttataggccg ctaaatcttc tttagtgatc aagccaccgt
    18541 ttttctgcat ctcctgggcg atctgttccg caatcgtgcc tttatagaat tcgtccgggc
    18601 cgttttcagc aatcatctcc aggctctttg ccaggttcgc ctgcaccagc gtgtcgccct
    18661 ttttcagcgg ctcgccctct ttccagaaga tagctttact gttttcgtga ttcggcaaca
    18721 cttcgctacc gtaggttttg agatcgtcag ccagcgcgtc gttaacgata aaaccatcgc
    18781 gtgccagttt aaacgcgggc tgcacgactt tgttcagcgg catggtgccg tatttatcca
    18841 gcgccagcga gaaacctgct accgtacccg gtgtgccgga agccagatgc gaagtgagtg
    18901 attttttgct gtccgggttg ccctgatcat cgaggaacat atcgcgggtc gctttggcgg
    18961 gtgccatttc gcggaaatcg atagccgtgg tattgccatt tttcgagcgg attaacataa
    19021 aaccaccacc gcccagattc cctgcctgcg gatgcgttac cgccagcgcg tagcccaccg
    19081 ccacggcggc atcaacggca ttcccgccct ccttgaggat atccaccccc acctgagtgg
    19141 cagtggcgtc cacagacgct accattccct gtttcgcgcg taccggatgg aagacatctt
    19201 cctccacacc atacgagacg ggcggcgcag gaggcgcggc ggcggcgcta aaacaacttc
    19261 ctgagagcag agcagcaatg gccacccggc gtaaaaacgt cggttttatc atcgttattc
    19321 tccagagatt aaggggcaac cccagctaag cctggtatat aactctgaat taatcatcgt
    19381 tttgtcggga agcgagtaaa cttaaaggat atcttcagag gaggatacgc gatgaaacga
    19441 cttctgattc ttacggcact cctgccgttt gtcggctttg cacagcccat taatactctg
    19501 aacaacccta accagccggg gtatcagata cccagccagc agcggatgca aacccagatg
    19561 cagactcagc aaatccagca aaaagggatg ctgaatcagc aactgaaaac gcaaactcag
    19621 ttgcaacagc agcatttaga aaaccagata aacaataatt ctcagcgggt gttgcagtca
    19681 cagccggggg agcgaaatcc cgcccggcag caaatgctgc ccaacaccaa cggcgggatg
    19741 ttaaacagca accgtaatcc ggatagttcg ttgaatcagc agcatatgct gccggagagg
    19801 agaaacggcg acatgctgaa tcagcccagc acgccgcagc ctgacattcc gttgaaaact
    19861 attgggccgt aaagttcgga ccaatcacgt caatcgcatc ggtacagatg caatccacgc
    19921 cccagcgcag caactctgcc gcgtgctggg gtttattgac ggtataaacc agaatccgta
    19981 atccggcgtc tttcaactgc atcactcgcg ctttatcgag taacttatga ttgagatgaa
    20041 tagagacgca gcccagccgc gcggtcagtt cgcgccagtc gtcgcgccac tcatcaagca
    20101 ataaaccacg cggcagttcc ggtgccgcct gttgtgcagc ttctaaagca tcaatctcaa
    20161 acgatgacag cagcggcggc gtcataccgg cccacagttg gcgtgccgcc agcgccacca
    20221 ttttgcccgt taatggtcca gtgccagtgg tgggtttgat ttcgatattc gccatcatcc
    20281 cgtgttcgcg acagcgttcc gccacctgcg aaagcaacgg cagcggctca cctttaaacg
    20341 ctttgctgta ccaactgccc gcatccacgc gcagtaaatc ctgccagttc agttcacccg
    20401 cgacgcccca gccgttgctg gtgcgttcga gattgtcgtc atggagcagg aagatctcgc
    20461 catctttcga taacttcgcg tcaaattcga tcatcttatg accgtatttt gccccgacgt
    20521 cgattgccgc cagggtgttt tccggggcca gcttaccgcc gccacgatga gcgacgatgc
    20581 ggggataagg ccagttactc atactcgttg tcctgtttca ccatcaaaaa gatgcagctg
    20641 attttccgcc agatgcagcc acagcgtgct gcctgccgtc gggcgctcct gatgcgccag
    20701 tcgcaccacc agcttctgct tgcgagctta gcgcaatatg ttccgggcga atgccgaggg
    20761 tcattttacg cccggcatac tgacggtagc caccgtttag cggtagcacg ataccgccgt
    20821 ccagttcgaa atgcgtgcct tcattattca tgcggcctgt cagcaggttc atcgccgggc
    20881 tgccgataaa actcgccaca aacaggctgg cgggcttttc gtagacttcc accggcgtgc
    20941 caatctgttc ggcaacgccg ccgttcatca ccattactcg ctgggcgagc gtcatcgcct
    21001 caacctgatc gtgagtaacg tagagtgaag tcgttttcag gcgacggtgc agctgttgca
    21061 gttcaagacg catctgtacg cgcagcttgg catcgaggtt agagagtggc tcatcaaaca
    21121 agaacaccgc cggatcgcgc acaatcgcgc gtcccatcgc cacacgctgg cgctgaccgc
    21181 cggaaagctc gcgcgggcga cgtttgagca gaccgtccag ctccagaatg cgcgccgctt
    21241 ctttaacgcg ctcggctatt tgctgcttgc ccatgccgcg aattttcagc ccccacgcca
    21301 tgttttcttc gacgctcata tgcggataaa gcgcgtaatt ctggaacacc atcgcaatcc
    21361 cgcgatcttt cggctccatt tcggtcacgc gctggtcgtt gatccagata tcgccttcgg
    21421 tcaccctctc cagcccggca accatacgca gcagcgtcga tttcccgcag ccagacggcc
    21481 cgaccatcac gataaattcg ccatccgcca catcaagggt cagcggttta atgacctagg
    21541 ttttgccatc ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca
    21601 ctatcgacca ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac tactggtggg
    21661 atcagggtta acaacatcgc cgccatcact gagttccatt ccgtggtgcc ttcgccagtg
    21721 gcgatcatcc ctttgatccc tgccacggtg gtgccgagat ccacgtcggt aataatcaac
    21781 aacggccaca aatactggtt ccagccgtaa ataaaggtga tcacaaacag cgccgccaga
    21841 ttagttttgg agagcggaaa aacgatgtcg caaaagaagc gcattggtga cgcaccgtcg
    21901 atccgcgccg cttccaccag ttcatccggc agcgtcataa agaactggcg aaacagaaac
    21961 gtggcggtcg ccgaggccat cagcggcagc gttaaaccgg cgtagctgtc gagcatcttc
    22021 aggttggcga tgacttccac cgtcgggaag atacgtactt caaccggcag catcagggtg
    22081 ataaaaatca tccagaagaa gaggttacgt agcggaaaac gaaaccagac aatggcaaat
    22141 gccgagagca tcgagacagt aattttgccg agcgtaatgc tgaacgccat cacaaagctg
    22201 ttaagcaaca tccgccagaa cggcgcgcta tttgtgccta ccccgttcac ccagatgttg
    22261 tggatgtttt ccagcagatg cgtgccgggg atgagcgtca tcggcgcggc gtagacggcc
    22321 tgtttatcca gcgtcgccgc gacaaacgcc acgtacagcg ggaagaggat caccgcgatc
    22381 ccgaggatca gcatggtatg gctgaatatc gtcagccacg gacggttctc aatcattggt
    22441 aacgcacctt gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca
    22501 tcagcaccat cgactgtgcg gcagacgaag ccagatccag tccggtaaaa ccttcgcggt
    22561 agatcttata aatcagcgtc gtggtggcct gaaccggccc gccggacgtg gcggcgtcga
    22621 tcaccgggaa ggtgtcgaag aaggcataca ccaggttcac taccagcagg aagaaactca
    22681 ccggggcgat aagcggcagc gcaatcttaa agaagcggcg aatcggccct gcaccgtcga
    22741 tggctgcggc ttcgatcaac gaacggggaa tggattgcag cgcggcatag aagaacagaa
    22801 agttgtagct gatttgcttc cacaccgagg caaacaccac cagaaacatc gcctgaccgc
    22861 tattttgcgc gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc
    22921 gaccggggtt aaacaggaag atccacaata cggcggcaac ggcgggagcc acggcatacg
    22981 gcagcaacat taaggtttgg tagaaacgac tgccgcgcac gatgtactcc accagcgccg
    23041 caaagaacag cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg
    23101 tccagaagga gtcgagataa tagctgtcat gaaacagcgt gacgaagtta tccagaccga
    23161 caaactggct ggagaaacca aacggatcga cgctttgtag cgagtaccac aacgcttcgc
    23221 ccgcaggcca gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcagataag
    23281 gcagccagcg cgagcggaac accggacggg atgatgacat tacggttaat tcctgaactg
    23341 tgccggatgc gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc
    23401 atccggcatt acactgatta agacttcgtc gatttctcaa agcggcgcag taactggttc
    23461 ccacgctcaa ctgcggtatc cagcgcctgc tgtggcgtct tcttaccggt ccacacgctc
    23521 tcaagctctt catccacaat cacgcggatc tgcggcatgt tgcccagacg aagccctttg
    23581 gtgaacggca acggcggctt gttcagcatc tgacgcgtcg cggtatccgc acctgggttt
    23641 ttctcgtaaa agccctgctc acgggtcagg tcatacgccg ctttggtaat tggcagataa
    23701 ccggttttct gatgccactc agcagcgttt tctggcttcg cgaggaagtc gagaaacttc
    23761 gccacgccgg tatacgtttc tttatctttg ccctgcatca cccacaggct ggctccgccg
    23821 ataatggcgt tttgtggcgc atctttcgca tcggcgtcgt aaggcatcat acctacgccg
    23881 tagttaaatt tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtggtcatc
    23941 gcgcaatcac cgttatagaa cttctcggtg gattcatcct tacgaccgac gtagctgaag
    24001 tcgcccttct tgttcatctc ctcgagcatg gcgatgtgtt tcacctgctc cggcttgttg
    24061 aactccagca ccgcgtccgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg
    24121 ttccaggcgc taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac
    24181 ttaataccgg aggctttcag ttttgcggaa tagtccgcca gatcctgcca ggttttcggc
    24241 ggctgttccg ggtctaaccc cgctttcttg aaggcgtctt tgttgtaata gagaacggaa
    24301 gtcgagctgt tgaacggctg ggagagtaag tggccggttt tgctgtcgga gtagtaaccg
    24361 gaaaccgtcg gcacaaactg cgactcatcg aactgaatcc ctgcctcttt aaacacgtca
    24421 tacaccggtt taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc
    24481 aaaatagccg gagcgttgcc ggtacgaaat acggcaatcc ccgcgcttaa attctgttcg
    24541 tagttgcctt tataggtcgg tacaattttg taatccggat tttcggcgtt aaaacgttgg
    24601 gccagagaat ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc
    24661 gtcactgcct gtgcattccc cattaacgcc agtccgagcg ccagtgctga agctgtataa
    24721 tgtaacggtt tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta
    24781 tgctcgcggg gtaacatgac atgctcgaat tacagaaaaa taactttttt gttacatttg
    24841 taagatagta aggtgtcaga aagatgacaa ggcggtgacg gcgtgggtga gggaaaatgg
    24901 gagatggggc acggataagc ggaaaaatag aaggtctgaa tcaaactcta cagattactc
    24961 atcgtttcat gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc
    25021 aatatattgc aactcaccca gtaggcctga taagcgcagc gcatcaggca attttgcatt
    25081 tgtcacctgt ctcaaaggag tcttttgact ccttatcaat caacgtgtta ttacccgcct
    25141 aaatacgcac ttctcactgc ttcgttcgcc agcagcgcgt cgccggtatc ggagagcacc
    25201 acatggccgt tttccagcac gtagccgcga tccgccagct ttagcgcctg gttggcgttc
    25261 tgctcgacga ggaagatagt catcccctgc tcgcgcagct gctcgatggt gtcgaaaatt
    25321 tgctggatga taatcggcgc aagaccgagc gatggctcat caagtaacaa caaacgcgga
    25381 ttgctcatca gcgcgcgacc aatcgccagc atctgctgtt caccgccgga catggtgccc
    25441 gcccgctgaa cacggcgctc atgcagacgt ggaaacagtt catacaccca ctttatgcgc
    25501 tcctgaaact ggtcacgttc ggcaaagaag ccgcccatcg ccaggttctc ttccaccgtc
    25561 atccgcgaga agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc
    25621 gccgtctgcc agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc
    25681 ggatcgccgc ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgaga
    25741 gtgacaatct cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg
    25801 tagtgggcgc tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa
    25861 ataggcacgg atcacgtccg ggttattacg gatttgctcc ggcgtaccgt ttgccagtgg
    25921 cgtcccctga ttgaccacat aaattcggtc tgaaatcccc atcaccagct tcatatcgtg
    25981 ttcaatcaac aaaatagtgg tgttgtgatg attgcgcagt tcggcaatca gctcatccag
    26041 ctctttggtt tcttttgggt taagacctgc cgccggttcg tcgagcatta aaatctccgg
    26101 ctgcgtcacc atgcagcggg caatctccag acggcgttgg tcaccatagg ccaggttact
    26161 cgcctgacgg ttggcgtgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc
    26221 gagcgcttcg ctctgggcgc gacggaaaga aggcgttttc aacaggccag agaacagccc
    26281 ggttttcagt tgctgatgct gcgccaccag caggttttca attaccgtca tttcacggaa
    26341 cagacgcaca tgctggaagg tgcgcaccac gcccatgcga gcaatttgct gccccggtaa
    26401 accttcaaga tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttgt agaaaccggt
    26461 caggcagtta aaaaccgtgg tttttccggc accgttaggg ccgattaacg agacgatttc
    26521 ctgcgggtac agttcaagat tgacgttgtt caccgccagc aggccgccga agcgcatcat
    26581 cagaccgtta acagataata atggctgact catgcctgct ctcctttcgc tgcgccgttt
    26641 ttcagcttca gttgcgggcg cgtcatgggc agcaaaccct gcggacgcca gatcatcatc
    26701 agcaccatca aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaattcg
    26761 cgcgacacca ccagcagaac agccgccaga atcactgcaa attgcgagcc cataccgccg
    26821 agcaccacta tcgccagcac aaaggccgat tcagcaaagg tgaaggattc cgggctgaca
    26881 aagccctgac gcgccgcaaa cagcgttccg gcaaaaccgg caaatgcggc gctgatggta
    26941 aaagcagtca gcttgatacg gcgcgggctt aagcccagcg aacgacaggc gatctcgtct
    27001 tcacgcaacg cttcccacgc gcggcccagc ggcatccgca gcaggcggtt aatgacaaac
    27061 aggcttaaca ccaccagcag caacgccacc aggtagagga agataacgcg atcggacgga
    27121 tcgtatttca ggccaaagaa attactgaac gtgtcccagc cgccttcacg agcggtacgg
    27181 ctgaactcga gtccgaagag tgtcggtttc gggatctgac tgattccgtt cgggccgccg
    27241 gtgatttcag tgttattgag cagcaatatg cgcacaattt cgccgaaacc aagggtaacg
    27301 atcgccagat agtcaccgcg cagacgcagc accggaaaac cgagcaggaa acccgccgcc
    27361 gctgccatca gtccggcaat cggcaaacag gtccagaacc ccagaccgta atagtgattg
    27421 agcagcgcaa aagtgtaagc gccgatggcg taaaaaccgc cgtaccccag caccagcaga
    27481 ccagaaagac caacaaccac attcagcccg agaccgagga taatgtagat catggtcaga
    27541 gtggcgatat ccaccgtccc acgtgacacc ataaacggcc acgccaccac aagcaccagc
    27601 agcgccacga ggaacagctt ttgcttcacc gtggacccat caatcgcggg cagaataaac
    27661 ttcggcccgg aaacgctttt caaccctttc tggaaagccg gtcgcaaaag ctggaagaaa
    27721 aagaccaccg ccgtgccgat aaacatccac tgccaacgga tgtctgaagc cgtgtcgacc
    27781 accagtttgg tgccatccag ctccagttgt acgcccataa agacgcccgc caggacaaag
    27841 aacatcgcgg cagagagcag tgccattgca atatgcatcg gtttcatact ttctctacct
    27901 ccgggcgacc cagaataccg gtcggcatca ccagcagcac cagaatcagc agggcgaatg
    27961 agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca
    28021 ggatcagccc gccaatcatc gcccctggaa tgctgccaat cccaccgagc accgcagcgg
    28081 taaaggcttt catcccggcc ataaagccga tataggggtt aatgacgcag tagaactgac
    28141 cgagcagtac gccagccact gccgccatcg ccgcgccaat aacaaaggtc aaagcaatca
    28201 cccggtcggt gttgatgcca agcaggctcg ccattttcag atcttccgcg caggcacgac
    28261 aggcgcgccc catacgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaagg
    28321 tgacaatcca gatcaccgcc tgcatggtgg taatagaggc agagaagttt tcgctatggc
    28381 ccaccaccca ctgaccgtta aacaggctcg gcagcgccac gtcgcgcgaa ccttcggtca
    28441 ggctgacgta gttttgcagg aagatggaca taccgattgc ggaaataagc gcaatcaggc
    28501 gcttagagtt acgcaccggg cggtaagcta cccgttcgat actccagccg taggcgctgg
    28561 cgatgacgat tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca
    28621 tcatcatcag cgcggcgatg atcataaatg agacgtagct gccaatcata taaacctcgc
    28681 cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca
    28741 gcgcgtaggt actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact
    28801 gctcagacat aaggtaacct ttctaaaccc gcccgcattt tacgggcggt gggatgatca
    28861 cttggctgcc gtggatgaac cgtcggcgtg ccactggaag acaccaaaat caaatccctt
    28921 aagatcgcct ttttcatccc agttcagcgg cccaatcacg gtgtttgcac cgttagcttt
    28981 taaatctttc accagcgcca gcggctcatc gctgccggta cgctcaaggg cagttgccag
    29041 agattgcact gccgcgtaag tgatccagac ataaggcccg gacggatctt tcttgtctgc
    29101 tttcagcgca tcaacgatgc cctggtttgc cggatcctgg tcatagcgtt ttggcatagt
    29161 gaccaacatg ccttcggcgg catcaccggc aatgttcgac agcgatgcgt taccgacacc
    29221 ttccggcccc ataaactggg ttttcaggcc aacggaacgg gcttggcgca gcatctgccc
    29281 catttccggg tagtaaccgc cgtagtaaac gaagtcgata ttttcttttt tcaggcgggc
    29341 gatcagcgcg gagaaatctt tctccccggc ggtaataccg tcgaagaaga cgacgttggc
    29401 gttagccgct ttcagcccgt cctgcaccga acgcgccagc ccttcgccat actgttgttt
    29461 gtcgtgaatg atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt
    29521 aggcccctgg gaagagtcca gcccggcagt acgcataatg tgttgataac cgcgttgggt
    29581 cagctccggg ttggtcgctc ccggcgagat catcagaata ccttcgtctt cgtagatatc
    29641 cgacgctggc tgggtagagg aagaacacag atgaccaata acgtatttaa tgccgtcgtt
    29701 aacgattttg ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac
    29761 gccaaccagc ttgtcgccct taattccccc tttgacatta atgtctttaa tcgcctgacg
    29821 cgcgccgtta aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac
    29881 ggcgacttta atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc
    29941 gatgatagtt ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc
    30001 tttgttttta tgtgttaaca aatcagactg ttcttttttt atactgcact gtttttgcct
    30061 gtctgattaa ggggttagcg cagtattttg tgatgatagc gattaaaatc cctatttttc
    30121 agtcgattaa gaacagataa tattctgaat ttattgatag ataaacagaa aaaagtgcct
    30181 ttgtcagcat aaaataacgg cacaaagggc ggaataattc actatcattc aggggattat
    30241 gctggacatt tttcattctc taatgtttta actttgtaat tattgctgtt aaaaaattaa
    30301 tcacctgcca aaagaaataa aaaagagaaa gcctccgatt aaattatttc gctacactgg
    30361 ttccactttt gtgatttaca cgggttaccc atgaagctga ccatcattcg attagaaaac
    30421 tttagcgacc aggaccggat tgacctgcaa aagatctggc cggagtattc cccttcctct
    30481 ttacaggttg acgataacca ccgtatctac gccgcgcgtt ttaacgagcg tctgcttgcg
    30541 gccgtgcggg taaccttaag cggcaccgag ggagcactgg attctctacg cgtgcgggaa
    30601 gtcacccgcc gtcgcggtgt ggggcaatat ctgctggaag aggttttgcg taacaatcct
    30661 ggcgtttcat gctggtggat ggcggatgca ggcgtggaag atcgcggtgt gatgacggcg
    30721 tttatgcagg cactgggatt tacggcacag cagggcggct gggagaagcg ttaatcgtca
    30781 agttgatttc aaaagtgatg accaacgaga aattgcctga tgcgctacgc ttatcaggcc
    30841 tataatgtgt gctgcaattt actgatttct ttggttcttg taggccggat aaggcgttta
    30901 cgccgcatcc ggcatgaagc aacgtattcg atattagcaa tttggcggca acccaaagtt
    30961 gccgattaac aattacttcg catcggtcgc cgtgccgttg gcgtgccagt caaatacgcc
    31021 gaactcaaag ccctttagat cgcctttctc atcccaggtc agtggcccca tcacggtatc
    31081 cacggagttc gcttttaggt atttggcgat ttcagccgga tcgtcagact gattcaggcc
    31141 cgcctgcaaa gattgcagcg cggcgtaggt ggtccagacg aatgcgccgc ttgggtcctg
    31201 ttttttcgct ttgatcgcat caacaatggg tttgttcgcc ggaacctgat cgtagttctt
    31261 cggcttggtc accagcagcc cttccgctga ttcgcccgca atgttagaca gcgaaacgtt
    31321 agccacacct tccggcccca taaactgagt tttcagccct gccgcgcgtg cctgacgcag
    31381 gatttgcccc atttccgggt gataaccgcc gtagtaaacg aagtcgatat tctctttttt
    31441 cagacgcgcc accagcgttg agaaatcttt ttccccggcg gtgataccat caaagaacac
    31501 cacgttagca ttgcctttct tcaggccgtc ctgcaccgct cgtgccagac cttcgccgta
    31561 ttgctgtttg tcgtgaacga tagcaatacg ctgcggtttc actttctcaa gaatatattt
    31621 cgccgccgtc ggcccctggt cggagtccag gccggtggtg cgcaggatca gctgatagcc
    31681 acgggcggtc agctccggcg cggttgccgc tggggtgatc attaaaatgc cttcgtcttc
    31741 gtagatgtca gacgcaggct gcgttgatga agaacagagg tgaccaatca catatttaat
    31801 gccgtcgtta acgactttgt tcgccaccgc aaccgcctgt ttcgggtcac aggcatcgtc
    31861 atattttacg atttgcagtt tgttgccttt aatgccgcct ttagcgttga tatccgcaac
    31921 cgcctgctct gcgccggtaa actcctggtc accgtactgc gcaaccggac cggacattgc
    31981 acccacgacc gcgactttaa tatcttctgc cagagccata ttgctgaatg ccagcgcgat
    32041 acatcctgcc agtaacgctt taccctttat gttcatcctg agaatcccca ttcttctggt
    32101 tattacgtgt gttgtgatgt tgttgttcag cactttattt cgttttatgc atgactaccc
    32161 gtgctttagc agcatactct gctaaaacat acccgatttt tatgatattg gaatagctat
    32221 tttgacagtt tattaacaat ctgcgtgggg attggcgttt tgccggaggg ggaattgatt
    32281 attgtagagg cccaaaaaac aaaaaccccg gactctcatc cagggttctc tgcttaatag
    32341 cggaaattac gcttcaatag cggcgcgaag ttttttcatc gcgttctttt ccagttggcg
    32401 cacacgttca gcggaaacac cgtaacggtc agccagttcc tgcaacgtgg acttgttgtc
    32461 ttcgtccagc cagcgcgcac ggatgatgtc ctggctgcgt tcgtccagac catgcatcgc
    32521 gtcggtcaga cggtttgccg cctgctcttc ccagttatca tcttcgatgc cgtcggcaaa
    32581 gttagatgat ttatcctgca gatagagcac cggtgccatc ggctggctgt cggaatcgtc
    32641 gtcggaagac aggtcaaagg tcatgtcctg tgccgccata cgtgattcca tctcacgtac
    32701 gtctttgctg gttacgccca gttcacgggc caccatttcg acttcatcct ggttaaacca
    32761 gcccagacgc tgcttggttt tacgcaggtt gaagaacagt ttgcgctgcg ctttggtggt
    32821 cgcaactttg acgatacgcc agttacgcag aacgtattcg tggatctctg ctttgatcca
    32881 gtggacggcg aaggagacca ggcgcacacc cacttccggg ttaaaacggc gcactgcttt
    32941 catcaggccg atgttacctt cctgaatcaa atccgcctgt ggcaggccat agcccgcata
    33001 attacgagca atatgaacaa caaaccgcag gtgagacagg atcagcgttt tagctgcttc
    33061 cagatcgcca tggtaatgca gcttttcagc cagcgcccgc tcctcgtcag ccgacaacat
    33121 cggccacgcg ttagctgccc ggatgtagga atccaggttg ccaactgggg ctaaagctaa
    33181 actttgcatt ttgtcagtca ttcaaatcct ctcaatcgat atcttctggc gcttcagtgg
    33241 tagcaacaac tgtgccaaag cttaagagca acgaggttat cattcactgt tttatcagac
    33301 cgtgatttta tccacaagtt caatgcaagc ttgtgaataa attacgcaca aaatgtgaca
    33361 tagagatgaa ataccgggaa gagacaacgg ggtctctttc cctgctacgg aacccattgc
    33421 agggaaagag tataacacgc ttttattatt caggcgtaaa gtggcgtaaa tgttgtaccg
    33481 tggcaagcca cgctgccacc cagccaatca tcgagcatac cagcagcaat agcaggcatt
    33541 catcgaacga taaaccattg atatcaaact tcgttccgaa aacctgtgcc acttccgcca
    33601 ccgctgacga caaacgcagt accagaattt ctgacaaaat taatgacaac aatgcgccag
    33661 aaaatcccag cagcgcaccg ccgtacagga acgggcgaag gatgaaacca tccgtcgcac
    33721 caatcagttt ctgcacgttg atggagtcac ggcgagcaaa gatgctcaga cgtacgctgt
    33781 tgccgatgac gaggaacact gccgccacca tcaacacgcc gatcatcgcc gaaacgcgcc
    33841 caaccagccc ggtcaacgcc gccagacggg caaaccagct gtcatccatc cgcacttcgt
    33901 caataccgtt aatctgcgtg atacgatcac gcagcgtatt gagtgattcc gtcccctgaa
    33961 aatcgagttt cgggatcacc accgccactg ccggaagcgg gttttcttcc agcatatcca
    34021 gcgcaccacc aaaaccagac cagttacgga actcgcccag tgcgtcttca cgagaaagat
    34081 agttcacttt ctccacgcct tgctcggcct gcaactgtgc caccacgccc gcagcggcgt
    34141 catcgtccag cgttttttgc aagtaaacag tgatttgcgg tgacggataa tactgcgtcg
    34201 ccgcctgatt aacgtttttg tacaccatat aacagacgct gggcagcgtc agggagatgg
    34261 cgataaccat caccgttaaa aacgtggcga acggtttgct tttcagatcc tgcaatgcgc
    34321 cgtggaaggc atagcgcacc tgttcgttga aaacgttggt tttgcgattt accggttttg
    34381 gcgaggattt cgcgcgtttc ggtgcgttac gaccgccgtc gcctgagccg ccgaccgatt
    34441 tacggaagcg atccagacgc ccaccaaact gccgaatatg attgattgca tcgcgcttat
    34501 tcatggccca cgcctccatg caagtgacca tcgctcaggg tgagcatgcg ataggaacgc
    34561 cgtgaaatca gattaatgtc gtgcgttgcc atcaatacgg ttaccccaac gcggttaaac
    34621 tcttcaaaca gacgtagaat cccttccgac aacgcgtcgt ccaggttacc ggtcggttcg
    34681 tccgccagca gtaccgcggg cttattcacc accgcgcggg caatgccaac acgctgttgt
    34741 tcaccgccgg aaagctgaat agggaagttc ttcgctttgt ccagtagccc gactttatcc
    34801 agtgccgccg acacccggcg acgaatatcg tcaccgctgg caccagcgat aatcagcggg
    34861 atcgccacgt tatcgtagac agtacggtcc atcagtagat ggtgatcctg gaaaatcatg
    34921 ccaatctggc ggcgcagaaa cggaacttca cggtttttca gacgcgtgat gtcatggccg
    34981 ctaaaccaga ttttcccggc gctgggccgc tcaatcccac agatcagctt caggagggta
    35041 cttttccctg cgccggaatg accggtcaga aacgccatct cacccggctg catatggaac
    35101 gtaacgccct gcagcgcctg tctcccaccg agataagcct tgctgacatg ttcaaagcga
    35161 atcattgtta atcctctcgg gcaaaaagtg cctctataaa gtcgtccgcc ttaaacggac
    35221 gcaaatcctc aatacgttcg ccgacaccaa tgtagcggat agggatacca aactggtcag
    35281 ccaccgagaa aattaccccg cctttcgccg tgccgtccag tttcgttagc gtgatgccgg
    35341 ttaagccaac agcttcgtgg aacagtttgg cctggcttac cgcgttctgc ccggtgctgg
    35401 catcaatagt cagcataact tcatgcggcg cttcaacgtc gagtttcttc atcacgcgga
    35461 cgattttctt caactcttcc atcagatgcg atttgttctg cagacgtccg gcggtatcgg
    35521 caatcaggac gtcgatatta cgcgctttag ctgcctgaat ggcgtcgaag ataacagagg
    35581 cggaatccgc accggtatgc tgggcaatca ccggaatatt gttgcgctga ccccagacct
    35641 gaagctgttc aaccgcagct gcacggaaag tatcgcccgc cgccagcatc accgatttac
    35701 cctgctgctc aaactgacgc gccagcttac caatcgtcgt ggttttaccc acaccgttga
    35761 cgcccaccat caggatcacg aacggcgttt tatcttcaac attcagcggc tcatcgactt
    35821 tcgccagaat ctcgcccatc tcttctttca gcaggccata gagcgcctcg gcatcacgaa
    35881 gctgcttgcg ggatgcgcct tccgtcagat tggtgataat tttacgtgtg gtttccacgc
    35941 ccacatcggc gatcaacagc tgttcttcca gctcttcaaa cagatcatcg tcgatttttt
    36001 taccgcggaa caggctgata aatccggaac cgagattttc tttggttttt aacaggctgc
    36061 gtttcaggcg cgcgaagaag ccttctttgg tcggtttttc ttgctcctgg gcaatttctg
    36121 caaccggctg ctcttcttct gccggaggaa ctaccatcac cgcctcttct gccgcttcgg
    36181 cagccagcgc ctgtgcttcc agctcttcgt cggtgatttc ttctttagcc gcttcttctt
    36241 ctgccgcttc gacaatctct acggtttccg cttcagcctg ccactcttct ggcgaaacct
    36301 cttcggcgtt gacgtcttcc ggcaacggca gctcttcacg ttcgatagcc actggctccg
    36361 gcgtttcttc tacgaccggt tccggctgtg caacaacttc cgcttcaggc tgcgcttttt
    36421 cactttcaac aacctgttca gtgacttcca caacgtcggc agcaaaagtt tccgcctcgg
    36481 cttcagtatg cgcctgcggc tgctcttcaa cggcttgttc agaagccttc gcaggctctt
    36541 gcgcctgaac gatttcttct acaaccggtt gttcattctg aacttctgtc tctttttccg
    36601 gggtctgctc tttttgacca aagcccagcc aggaaaaaaa gccacgtctt ttttctttcg
    36661 ccatttgcga ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata
    36721 gcagctaaaa aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa
    36781 gtatgtgtta tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc
    36841 ggccaaatcc gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagc
    36901 ccaggtctgc gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg
    36961 gtcattgttg acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa
    37021 gcgttatcgc gctacgctgc gggggcaacg ttgattgaga tggatcgcgt ggtttctcag
    37081 cagttaatta agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac
    37141 gcaatgtcat tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg
    37201 ttccgccgtg gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct
    37261 gacgaagccc tgatttatgt cgaaagcgaa gtcgaaaacg gtctgcccac tgttccggca
    37321 aactggtcat tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacac
    37381 gaagcacaag gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgcgtttg
    37441 gggattttta atcctcaatc tggtgcatcc cttcccgcgc ccgctaaata ttttcgttaa
    37501 cgtggcgctg atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac
    37561 tttaccgaaa gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg
    37621 cgtgtttgaa ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc
    37681 gccaacaaag ctaacaaagc gcgttccttt atagctcagc gtacctttat cgcccactgt
    37741 cagggcgtgg tactgctggg cgtcgaggcg aaacgtctgc tccattcctc cgctttgcgg
    37801 tttgaagctt gcctcatagc gcatactggt gcctgccgga gtcacttcct gctggcgcga
    37861 acggcgatcg ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag
    37921 cggagccata tcattatcag ctttttcccg tcgctgttgc ataaaacgaa acgatgcggc
    37981 gacgacaatt aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc
    38041 tcatcggaaa atgcggaatt aagcataccc tgccagttat ggtgttgtca tccgtccacc
    38101 ctcgccacta aactggaagc aagaccgtag gcattctgct tacgaaaaaa taacgaattc
    38161 aaggaactaa gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg
    38221 atgcatcgta tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc
    38281 tcctgctgtt actggcctgg caagcaccga tgttcgacgc cattagctat ctggtgctgg
    38341 cgggactgtg cgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga
    38401 tgtacgccat cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc
    38461 agatgacgct ctctttcttc tggcctctgc cactggtgtt gctggttctg ggtgcgctgc
    38521 tactggcgat tatctggacg cggctggaag agtaccgttg gcctatctgc acgtttatcg
    38581 gcatgacgct ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc
    38641 cggcgctctc tgcgtttgtt ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg
    38701 ggagccacta tcgccgacgc ttccgtgcgg ataacgcgat tgctgcggcc tgctactttg
    38761 ccggtcactt cctcatcgtc cgctcactgt atctctgata aaacttgact ctggagtcga
    38821 ctccagagtg tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgaca
    38881 cctgacaatc acggcaagaa agcccctcaa tttgctgcgt tcaaaccgct aaccacggta
    38941 cagaacgcca acgactgttg ctgcgacggc gcatgctcca gctcgccaac gctctctgaa
    39001 aacgtctccg gcacccgcta tagctggaaa gtcagcggca tggactgcgc cgcctgtgcg
    39061 cgcaaggtag aaaatgccgt gcgccagctt gcaggcgtga atcaggtgca ggtgttgttc
    39121 gccaccgaaa aactggtggt tgatgccgac aatgacatcc gtgcccaggt tgaatctgcg
    39181 gtgcaaaaag cgggctattc cctgcgcgat gagcaggccg ccgacgagcc acaagcatcg
    39241 cgcctgaaag agaatctgcc gctgattacg cttatcgtga tgatggcaat cagctggggt
    39301 ctggagcagt tcaatcatcc gttcgggcaa ctggcgttta tcgcgaccac gctagttggg
    39361 ctgtacccga ttgctcgtca ggcattacgg ctgatcaaat ccggcagcta cttcgccatt
    39421 gaaaccttaa tgagcgtagc cgctattggt gcactgttta ttggcgcaac ggctgaagct
    39481 gcgatggtgt tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagccgc
    39541 gcgcgtcagg gcgttagcgc gttaatggcg ctgaaaccag aaaccgccac gcgcctgcgt
    39601 aacggtgagc gggaagaggt ggcgattaac agcctgcggc ctggcgatgt gattgaagtc
    39661 gccgcaggtg ggcgtttgcc tgccgacggt aaactgctct caccgtttgc cagttttgat
    39721 gaaagcgccc taaccggaga atccattccg gtggagcgcg cgacgggcga taaagttcct
    39781 gcaggagcca ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc
    39841 agcgccattg accggattct gaaactgatc gaagaagccg aagagcgccg cgcccccatt
    39901 gagcggttta tcgaccgttt cagccgtatt tatacgccag cgattatggc cgtcgccctg
    39961 ctggtaacgc tagtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa
    40021 ggtctgacgc tgctgctgat tggttgcccg tgtgcgttag ttatctcaac gcctgcggcg
    40081 ataacctccg ggctggcagc agcagcgcgt cgtggggcgt taattaaagg cggtgcggcg
    40141 ctggaacaac taggtcgtgt tacccaagtg gcgtttgata aaaccggtac gctgaccgtc
    40201 ggtaaaccgc gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg
    40261 acactggcgg cggcggtcga gcaaggcgcg acgcatccac tggcgcaagc catcgtacgc
    40321 gaagcacagg ttgctgaact cgccattccc accgccgaat cacagcgggc actggtcggg
    40381 tctggcattg aagcgcaggt taatggtgag cgcgtgttga tatgcgctgc cgggaaacat
    40441 cccgctgatg catttgctgg tttgattaat gaactggaaa gcgccgggca aacggttgtg
    40501 ctggtagtac gtaatgatga cgtgctgggt atcattgcat tgcaggatac cctgcgcgcc
    40561 gacgctacaa ctgccatcag tgaactgaac gcgctggggg ttaaaggagt gatcctcacc
    40621 ggcgataatc cacgcgcagc ggcggcaatt gccggggagc tggggctgga gtttaaagcg
    40681 ggcctattgc cggaagataa agtcaaagcg gtgaccaagc tgaatcaaca tgcgcccctg
    40741 gcgatggtcg gtgatggtat taacgacgcg ccagcgatga aagctgccgc catcgggatt
    40801 gctatgggta gcggcacaga cgtggcgctg gaaaccgccg acgcagcatt aacccataac
    40861 cacctgcgcg gcctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc
    40921 cagaacatca ccattgcgct gggactgaaa gggatcttcc tcgtcaccac gcttttaggg
    40981 atgaccgggt tgtggctggc agtgctggca gatacgggtg cgacggtgct ggtgacggcg
    41041 aatgcgttaa ggttgttgcg caggagataa ggcaaaccga tcgcaacatt gagcgcgatc
    41101 ggtcccctcg cccctctggg gagagggtta gggggtgagg aaaaggcggc atcgaagcca
    41161 atcagcccct atcaaccgcc tttacgaatc aaataacgat aaggcagtcc atccgtctct
    41221 ttcgcaacca gttcgtgttc cataaaggta caaaacccag gaatatcgcg ggtagtggcc
    41281 ggatcgtcgg cgataatcag caacgtttcg ccaggctgca tattgcgcac ggttttgcgc
    41341 accatcatca ccggttccgg gcagcgcagg ccaagcgcgt cgagtgtgtg gtcagggctg
    41401 gaaaagagat cggtcatttt cttctcatca cttaaaaaaa cggcgctagt ttacgccctg
    41461 tgagtccgta agcaaaccag gttaacgatt gcgtgaaaat taaccattgc attgtcaacg
    41521 taaagcagta tcatgcggcg gctcgaaaaa agggtaagca cgttattatg ttaaggtaac
    41581 agacgtgtcg tacgtattgg gttccctcac cccaatggtt aatcaaaaag gtacaatatg
    41641 aacgttttct cgcaaactca acgctataag gcgttgttct ggttatcgtt atttcatctt
    41701 ctggtgatca cctccagtaa ctatctggtt cagcttcccg tctccatttt tggtttccat
    41761 accacctggg gcgcgtttag ctttccgttt atttttcttg ctaccgacct gaccgtgcgt
    41821 atttttggcg caccgctggc ccgacgcatt atcttcgcgg taatgatccc tgcgttatta
    41881 atctcctacg tcatctcgtc gctattctat atgggttcct ggcagggatt cggcgcactc
    41941 gcccacttca acctgtttgt cgcccgtatc gccaccgcca gtttcatggc ctacgcgctg
    42001 gggcaaatcc tcgacgtgca cgtttttaac cgcctgcgtc agagtcgccg ctggtggctg
    42061 gcaccgacag cgtccacact gttcggtaac gtcagcgaca cgctggcctt tttcttcatt
    42121 gccttctggc gtagcccgga tgcctttatg gctgaacact ggatggaaat cgcgctggtc
    42181 gattactgtt tcaaagtgtt aatcagtatc gttttcttcc tgccaatgta tggcgtatta
    42241 ctcaatatgc tgttgaaaag actggcagat aaatccgaaa tcaacgcttt gcaggcgagt
    42301 taaaggttcg ttatcagagt tgtgataaga tggatgaatg agccgttatg gccgtttatc
    42361 gaaaggaaga agtcaatgcg caatctggtt aaatatgtcg gaattggcct gctggttatg
    42421 gggcttgcgg cctgtgatga taaagacact aacgctacgg cgcagggttc ggttgcggaa
    42481 agtaacgcta ccgggaatcc cgtcaacctg cttgatggca agttaagttt ctcgctgcca
    42541 gcggatatga ccgaccagag cggtaagctg ggaacgcagg ccaataacat gcatgtctgg
    42601 tccgacgcca ccgggcagaa agcagtcatc gtcatcatgg gcgatgatcc gaaagaagat
    42661 ctggcggtgt tggcgaagcg tctggaagat cagcaacgta gccgcgatcc gcagctgcaa
    42721 gtggtaacca ataaagccat tgagctgaaa ggtcacaaaa tgcagcagtt agacagtatt
    42781 atctccgcga aaggccagac ggcgtactct tccgttattc tgggtaacgt gggtaatcaa
    42841 ctgctgacca tgcaaattac gctgccagct gacgatcagc aaaaagcgca aaccaccgca
    42901 gaaaacatca ttaatacgct ggttattcag taagttttaa gatgatgagg cggcctcagg
    42961 gacgtgttcc ggaggccgtt tttttaatcg ccacgtcagt aataacgcga ttgcgaccag
    43021 tcccgccgcc gccagataaa tcaccggtac gcccgcccag ctcatcacca gcccagccag
    43081 tggtccagtc acgccaagcg ataaatccat aaatacggtg taagttgcca gcgccgcccc
    43141 ctgattttgc tgcggaaccg cttttaccgc cactacaccc aatgccggga acaccagcga
    43201 aaacccggcc cccgccagta agacgccgat tttcgccatc cacggcatag tcgccacgcc
    43261 aaccagtagc aggccgatta tctcaacgct aaagcaaatc atcgctacgt ttaagccacc
    43321 gatacggtta atgccgttag ggaataacaa acgcgtaccg acaaacgcac agctaaacag
    43381 cgtcagcgcg aaagccgcac cgtcccaacc tttagcgtca taaaacagcg tgataaaggt
    43441 ggcgatgacg ccaaatccgg cggaagccag tgccagcgcc ataccgtaca gccagacgcg
    43501 cccaagcacc gcgcgaaacg gcagcggttt gcctttactg gcttttaccg tcggacgcgg
    43561 gatcgccaac aaaatggcca ccagcgccac gcccataatg attaacgcta acgcctgcaa
    43621 gccgccccag tgataaaaca cgacgcctaa cggcgcaccc atcgccatcg ccccgtaagt
    43681 gacaatgccg ttccacgaaa tcacccgccc gatatgcagc gagccaacca ctccaacgcc
    43741 ccatagggtc gatcccgttc cggcaaaact ttgcccaatc ccaaggatga cgcgccccag
    43801 gcaaagtaat aacaggctga tgacaggcag actggcggtt aatcctgccg tcagataccc
    43861 cagaccgctc aaaaagcagc cgcataaacc gaagacgaca atctttttgg gtcccagcaa
    43921 atcggcgtaa cgtccggcat gagggcggct cagcaaggtg gcgaaatatt gcaggctgat
    43981 aaccaatcct gcccagaagg cgctaaagcc catcacatca tggacatagc ccggtaatac
    44041 agcgagcggc aacccgatgg tgaggtagct ggcgaagtta aacatgacaa tggagacaat
    44101 gcgcaaattc aggcgcaatc cgtttagcgc gggttcggct acgggttcgg gcatgaggat
    44161 caccacattt ttacaacagt gtttcatttt taccatgtgc tgacgtgaaa atcagcagta
    44221 agaatcagaa tattgctggc gtgactcccg cgctacactt aacacaaaaa gtcacaagga
    44281 agccccaatg gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc
    44341 ctcgctggta gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt
    44401 actggcgctg ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg
    44461 agtacaacgc cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc
    44521 gctggtcggc gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa
    44581 taaggagctg acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca
    44641 tatgtcgccg gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac
    44701 agcgctaatg accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt
    44761 agtttttatg ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa
    44821 cccacagatt cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc
    44881 gttaaagacg ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat
    44941 gggcgtacag tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa
    45001 tatcggcgcg gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt
    45061 ttacgaatgt attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat
    45121 tttagaacca cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc
    45181 attgttaatc tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac
    45241 cagcgtgtgt aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact
    45301 ggggccgggc agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca
    45361 ctttcatttt ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc
    45421 gaccttaagc gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg
    45481 acgcgaacgc tggctggcgg ggcgtacatt gctttcgcac acgctttccc cgctaccgga
    45541 gatcatctat ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa
    45601 cttaagccat agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg
    45661 cgatatcgaa gtgattcgcc cgcgcgtcaa ctggcgctgg ctggcgaacg ccgtgttcag
    45721 cctcggggaa cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg
    45781 gcgcatctgg acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat
    45841 cgtcagcgta gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa
    45901 tttaagcctt gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg
    45961 gatcgattca gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta
    46021 atcagtatga cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt
    46081 ggggttacca tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct
    46141 tttatcgtcc atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg
    46201 ccactaaacc cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa
    46261 ccattggtga aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact
    46321 cattcagaag atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac
    46381 ggtgaaccgt tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt
    46441 caacgtcacg cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa
    46501 acagagctgc aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg
    46561 ccccgtcctt tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga
    46621 attaaagcgc cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac
    46681 gatgtcttcg tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc
    46741 tttaacgtca tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac
    46801 ctgctgtacg gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat
    46861 ccggcttacc acacccaact gtcacagccg atcgaaaccg tgatgctggc gctcaatacc
    46921 gccaaagccc ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa
    46981 aaatcgctga ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc
    47041 ccttctgtgc cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg
    47101 aaagagttgc tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa
    47161 aatggtcagc cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg
    47221 atggcggaaa tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc
    47281 gaagaagaga gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac
    47341 cgcacctggg gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg
    47401 cacgctgact tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc
    47461 ggcgaagtgc tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg
    47521 acccgtctgc atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta
    47581 tcaaaaccgg agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa
    47641 cagattaaac cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga
    47701 tcccgatggt gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg
    47761 acccggcgct cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct
    47821 ctacccgcac catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt
    47881 ggaaggcgct gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg
    47941 atatgctgaa cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc
    48001 tgctcacttc cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt
    48061 tcgccgtgcg ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt
    48121 tgctagtgat ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct
    48181 ggcagcacat cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc
    48241 gactgctgcg cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc
    48301 tacgcggcct gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc
    48361 cgatgatcac cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg
    48421 aaaacatctt tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg
    48481 actatccggt gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt
    48541 tgattgtcga tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc
    48601 acgcgtgaac tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc
    48661 cctgctggcg ctgattgcgc tcaccagcca gtggtggcag ccgtacgatc cacaggcaat
    48721 tgatttgccg tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt
    48781 aggtcgcgat attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat
    48841 ggcctgcctg ctgctggtgc tgacattagg gctggttatt ggcggcagcg ccgggttgat
    48901 tggcgggcac gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac
    48961 ctcgattctg tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat
    49021 cgccatcgcc ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc
    49081 actacgccaa cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt
    49141 gtttgtcgat catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga
    49201 tatcggccat atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc
    49261 gccgaccgcc gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc
    49321 gctgcaaatg ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt
    49381 gggtgacgca ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg
    49441 ccgcaacaga ttgaactacg taatatcacg ctacaggccg cgcagccgct ggtacacggt
    49501 gtatcgttaa ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg
    49561 aaatcattaa cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg
    49621 ggggaaattt tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt
    49681 gccaccatca tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac
    49741 gcgcgggaaa cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc
    49801 atagaagcgg tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg
    49861 agcggcggca tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt
    49921 atcatcgccg atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat
    49981 ctgctggaaa gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg
    50041 ggcgttgtgg cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa
    50101 cagggcgatg tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt
    50161 tccgctcatc tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg
    50221 gcctttccca tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc
    50281 tgaataacgt ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg
    50341 gctgcggaaa aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga
    50401 atattagctg gcacggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc
    50461 gccgtgatat tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg
    50521 tgcgcgagat cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac
    50581 tggcgcgcgc cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac
    50641 gcccaccgca gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg
    50701 tcgaaccgaa attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg
    50761 cgggcgtcat tcgcctgctg aaaaagctac aacaacagtt tggcactgcc tgcctgttca
    50821 tcactcatga cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg
    50881 gacaaatcgc cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac
    50941 gtgtgctaca aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg
    51001 tttaacgcca atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga
    51061 cagcctgagc cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg
    51121 tagtgccctg gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc
    51181 gtatgtgtat gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca
    51241 tcaccacgac ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga
    51301 aatcgccgtg ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc
    51361 ccagcgcggc gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc
    51421 taaaacaagg aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca
    51481 tagatagatc ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg
    51541 attcaaacgc tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc
    51601 agccgcatta ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat
    51661 cttttctgta cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg
    51721 cagggaaagc tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata
    51781 acctgacaag cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac
    51841 agctggttgc ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc
    51901 cctatactta gtaaccagtc acacacgctt tctacagcgc ctttatcggt atctggcgac
    51961 ggatgaggcc gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct
    52021 cttccttcct taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg
    52081 atcccttgag gaacaggagt tttaaatatt tgatccaatg tatttttttg tttcttccgt
    52141 aaagcctgta ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt
    52201 atgaagtcaa gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag
    52261 tgcgatggtg aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat
    52321 ttcgaatccg gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg
    52381 catggtcagc ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc
    52441 ggaaagcatt tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga
    52501 acgcgctatc gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac
    52561 gcccagcata aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat
    52621 cagcaccagc gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc
    52681 catcatgatc tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc
    52741 acgctcgcgg atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat
    52801 gatcgccatc accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt
    52861 ttccagcgat accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc
    52921 accgttgata atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc
    52981 atcgacgttc acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg
    53041 cggaatattt atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc
    53101 ggtgatcatc tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga
    53161 taactgcgat tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt
    53221 aacggtcgct gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc
    53281 tttatcaccg agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaaatg
    53341 gcgcatcatc cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg
    53401 gctatcagca acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac
    53461 gttccgcggg cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca
    53521 ggcccttcca gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat
    53581 gtgataatcg ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc
    53641 agccccattc cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa
    53701 ctgcctttat gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc
    53761 agcatcccca gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg
    53821 ggggtcacgt aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg
    53881 cttagcattg acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc
    53941 aggcttttta cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg
    54001 ctggcgggtg tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc
    54061 acgtaacctt ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt
    54121 tccacaggcg taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc
    54181 gtgatatcgc ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt
    54241 tcgataaagt aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg
    54301 tcgcggtcga gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt
    54361 atcagcatca ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc
    54421 agttccagcg cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc
    54481 ggcgcgtggg tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc
    54541 gcttcctgca aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc
    54601 agttcctgcg gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg
    54661 caacgttccg cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc
    54721 gagagatcga ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta
    54781 ggctcatcga ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc
    54841 tggcgaatgc cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa
    54901 cgctcgctca tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg
    54961 gcatgtaact ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac
    55021 atatagccca cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc
    55081 cacgcctcac cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta
    55141 ccgcagccgt tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg
    55201 tgatcaacgg caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc
    55261 tctgcgtttt caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct
    55321 tgcggtaaca gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc
    55381 tcttcggcgc tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag
    55441 cgttcggcct cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg
    55501 cgaatactgt cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt
    55561 ggctcatcaa ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt
    55621 tgcttcatcc cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg
    55681 ctggtcagca gctcattaat tcgcgcttcc cgctccgctt tatcgtgacc gaacaggcga
    55741 gcgaaaaaat cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc
    55801 tgcggcatcc aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg
    55861 ccacccagca ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat
    55921 aaactcgact tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata
    55981 tcgagagtga tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt
    56041 tgcgcgacag gcgggacggg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga
    56101 ggtcgtcagg ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg
    56161 ttttgacata ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga
    56221 acatcagttt cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac
    56281 tggcgacaaa actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc
    56341 gggcttcacc gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat
    56401 agacgtcgct gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg
    56461 gctcggcaac ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga
    56521 tatctgcggc aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa
    56581 tgatattggt gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg
    56641 attccagcgc agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag
    56701 aaatagcccc tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca
    56761 gttcggcctg gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct
    56821 ccagcaaagc ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt
    56881 ccagtcgctg ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa
    56941 caaactggcc ttctttcacc agaatggtgt cgatacgccc ggcaattttg ctggcgatat
    57001 ccacttccgt cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg
    57061 ggcgcaacag ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg
    57121 ccagatggcg cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg
    57181 cgtgttacaa ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa
    57241 acgacaaata cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagtgaaaac
    57301 gggtagtaat gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa
    57361 gtgtgatcta taaaactggc tgacaaaacg gtatccatcc tgttggatcg catttcacgc
    57421 ctgttcagtt gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat
    57481 agtaacatta atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat
    57541 caaaggcata agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat
    57601 ctctggccct gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg
    57661 tttactgtca cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc
    57721 accatttccg ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag
    57781 tgcttcaagt ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca
    57841 agagatagcc cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt
    57901 aataatttcc gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt
    57961 tatcggtcct ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc
    58021 cattattttg tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt
    58081 atcctcatca agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt
    58141 ttcatgattt ccagctaata catgaacatt ttcattgata ctcattatgc gttttaacaa
    58201 atcgatgata aattgatcgc cacagatatt actaaatcga tcgccggttt ggtcgccaag
    58261 aaacgtacat ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa
    58321 aacattatct cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc
    58381 attaaagttc tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct
    58441 aaggaaattt tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa
    58501 aactgcaccg actgagccat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc
    58561 aacaggtgaa caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc
    58621 gaatttaata tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga
    58681 ctcagtaact gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac
    58741 aatgctgcac caattaatat cggtattatc aagagggcaa ccgtaatttt tactattaat
    58801 tgtcacaata aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag
    58861 catactatat ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat
    58921 agattgttgg aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat
    58981 tgtcctggca atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc
    59041 tttagtgaag ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact
    59101 gccattagtg cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag
    59161 gaatacatag ttaacatttt atcttatgac atacacctca tattcgatgg ggaaataatt
    59221 atattttgca caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag
    59281 agaaagtccc taaatattgt agaggcgtca tcagtacata cctctacaat cgattaagaa
    59341 aacctatgaa atactggcaa aattaaaaag agataccaca gcactcatga agttttattg
    59401 aggttgaaaa caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat
    59461 cttccaagaa tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa
    59521 tccataatgg ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc
    59581 ttcaggtttt ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg
    59641 tgctatagca ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt
    59701 tttattacat atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg
    59761 aacctgcaaa gcaccgagac aataacattg attttcttca tttcttatta ttccgtgatg
    59821 actggttaaa actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt
    59881 atacgtatca ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa
    59941 attgatttca tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt
    60001 accctccata tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt
    60061 actaaatcta tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt
    60121 taaaaaaaca tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta
    60181 gagcaattta ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa
    60241 ttcacataat aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga
    60301 ataaagtaca gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg
    60361 tgggttaatc acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata
    60421 gctatcaaac ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt
    60481 aaaattaccg ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt
    60541 ataaattatg tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc
    60601 aatcttaact gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt
    60661 aaagataaac gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga
    60721 tggctcacta ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca
    60781 gtgaccatgt tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc
    60841 attatccagc ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg
    60901 gtgttgatga ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt
    60961 aaatatattg tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa
    61021 ataaatgata catgtctgaa agacatattt acgacgatat aaaacaataa tacttatatg
    61081 ttcgctgatt ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca
    61141 tccctggaag acaattgtaa aaaaaaacaa cagaataaga attaatctta ggataaatat
    61201 ttatttatca tggccttttg accgctaaaa aatggcactc ggcacagtaa accccgctat
    61261 agttatgcta tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta
    61321 tcattaatgc acatttcaat attagcaggg atacctgagg agtttaaagt gaacatatat
    61381 atcgggtagc ttttcaaatt aatccctttg attataggct taatttgcat cgcgttaggt
    61441 ggctttgtgc tggaaagttc agggcaacgt cacgggtcat gtgctgattt ctctggcggc
    61501 catatgcctg gcattattca ctaccgcatt tattatcatt tcgcaactca cgcgcggcgt
    61561 taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat
    61621 gatatggggt tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt ttgttgccgg
    61681 ccatgttatt tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg cagcgtcgtc
    61741 cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgacg gaacaccggt
    61801 acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct
    61861 gctcggtttc atttggtcta ttacgctgtt acatagtgcc gacataactc cgcattatgt
    61921 cgccttgggt taaccgcaat ctgtgcctgt ctaattggcc tagtggccac aatcgtccat
    61981 caaacacgta atacgttctc atccaaagag cactggctgt ggtgttattg ggttattttt
    62041 ctcggctcag tcacggtgct gcaggggata tacgtcttag tcagttccga tgcaagcgcc
    62101 cgactggctc ccggcattat tctcatttgc ctcggaatga tctgttacag catattctca
    62161 aaagtctggc tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg
    62221 atgataccgg tatttacctg tctgttttgc ttgtttttgt catcattcct cgcggaaatc
    62281 gcacaaactg agctggacta ctttattcca tctcgggttc tggttggatt aggcgcagta
    62341 tgttttacat tgttctctat tgtttcaata ttagaggccg gttctgcgaa aaaaaattaa
    62401 aatttacata atcccagcgt tattcatatc ttaacgctgg ttttttacga acttagtgaa
    62461 ataatgtaga atataaaatt ataattttat attggagcac atgtgttaat gatcaacccc
    62521 gggatactaa aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt
    62581 aaagatgcat cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc
    62641 gaaactttgg ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa
    62701 aaatacgaat gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa
    62761 ctaccgctac catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga
    62821 tctgttttag catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca
    62881 cgagccttcc agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata
    62941 aaatacattt tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc
    63001 attagaggat tttatacatc ttgataaata ccgctgccct tccccaagca aataatgcat
    63061 actatttaca tcatatataa aatttgcacc tggcacacag ggtaaatgct ttttcgaaat
    63121 ttcagcggcc tctatttgag atttagcgag gccaatctcg ttaagattct ctgaaagata
    63181 taaatcacag gtgtcaatct tacctgattt aaccaatgga taatccgtta tactgtaggt
    63241 atctttaaaa gccacggcaa acatttcaat aataacaatt actaccacaa gcgaaatcaa
    63301 aatgcgaaaa agaggaatac tccttttgcg ctctactttt tcaacaacat cttcttgtaa
    63361 atgagaatca tcataagatt gtttacttat tgttatgttt tcactgatgt aaaagcctac
    63421 tttgggtatc gtaagaatca cattttcttc aacaccaata tttttaaaac ttttgcgtaa
    63481 cagggatata tattggttca atgtgttgtt tgacagatca aatgaaaatc tctccaaaac
    63541 gctatcaatg attgagttcc tgtcaaccgc tccaccatta ctttccagta acactgccaa
    63601 aatacacccc gccgtagctg gaaaatttat ctcatcctgg gtatttacat tcgagagcga
    63661 gcatttgttc ggatcaaaaa taatcgcgtc attgataatg aaaaaacatc tccgctccat
    63721 gcaggcaaca acacaaaata agattttgtc attttagcct attcctggtg gaactgacac
    63781 aagaaactaa aatacaaata taaccctgaa aaagtcatat aaaacttata tccacagaat
    63841 aaaaaatcaa acatgtcagt tttatcaata taaagtatcg caaggcgaaa agtgatgcaa
    63901 gaatactcct aagtaaatga aagcgcacca catgaaatta tcaacacatg taatttcatc
    63961 attaggtgct atcgaaaata actcaggagc tacttaagat gaataatgtc ttactcatga
    64021 gacccgatga ctcagaattt atgatacctg atgactggtc agggctaaac gcaaaaggca
    64081 ttacatacga acaagataat agagtgacag agtcttttct gccttttaaa tctttttatt
    64141 gttcaccggt gtgcacgaca gttgaactca tctcacaaaa taattgtttt gctatgggta
    64201 tccgctacct tctggaaaag aaaagagact taaggctaaa tcactttaaa gccttctctc
    64261 ataaatgcga gacaaaacct gacgtgttaa ttatgaccgg gtgcaccgtc gctgatactt
    64321 atagtatatt ctcacgcatt gttgaagaaa atataatgaa aacgatgaca cctatcgtca
    64381 tcattactgg gcatcactca gaagatcttt gttctctggc atcgaaactg ggaggagtta
    64441 aaataatatc accgacagaa aagattgcaa aaatagaaaa cgctatcaat gatgctttta
    64501 acagcaagcc taaaaaaata agatttttaa cagatatact ctctcctcaa caaattgaga
    64561 ccatgaaaat ggttgcgcag ggtagatccg ttaatgagat ttcagccgca atgaatatta
    64621 gcatcaagac cgtgtatgca cataaagttc gtgctatcaa taagctggga attaagaaaa
    64681 aagtttatga agcgcttttt tataaagcgc taattcagtt caactgatgt aaaaatcacc
    64741 ctcgcttaat aaataatata ttctatgtct ttttttataa gttaaaaaag gcgggatatc
    64801 aaatcccgcc atgaaattaa ctaatcatct gcattattct gcgtagatat aagcctgggt
    64861 acggatggtg taagagtctg gctcaacaac ctgttctttc atatgggaaa cgacgcgata
    64921 ggcaccacct gttccagtat ttttcagcat catataacgc ttaccgccaa tctcttcgga
    64981 ggcagctgaa ggaggatcct gctcaagcgt tgtcagggaa actttcagtt tatggtccgg
    65041 gttattgtca ccagaaatta ctgcatccgc tgaagtactg ctctccgcat aaatatcatc
    65101 cgggaaagtt acagcatagt cgtggctctg cccatcagat gcgccaacaa tacctgttgc
    65161 caaaggcgtc catgcagtta acgtaccgct atatacccct ttcactggtt gaatggtatt
    65221 attaacaacg ataggcttgg tcaaagtaag attagctgac gcttcagtac cctggcttac
    65281 taaagttccc gcctgagaag caaaagctgt ccccgccagc atgattgcta acaatgtttt
    65341 cttcattata ttttctccat gattttcatt tacactgcaa aatgcagttt ctttctttgc
    65401 atttacgtgc tatcgcgaaa atgcttccac caaataatgg aaacatcttc acaaaaataa
    65461 attatgagtt tcttaattta gaaactataa acatgtcgcc gctatctggt ataaccctga
    65521 tgcacttttc ttcgcaggaa gttggtattt tgcgatacag gattgatctt tagaataacc
    65581 ccatttcaca ttaaactcgc cttccgcact caagccagaa acataagcaa taccatcttc
    65641 acctacaatg ccggccatat tttcagtgtc tttgctataa ctaatcacag cgccgaaggg
    65701 gatgggttta ccctctttct gttttaaggt tactaatcct ttatccccgc tcttcactgc
    65761 caatgtcgca ggcacaacgg ctccagctgt cggtacaacc aattgactag tttgtggtaa
    65821 gtcgtaattc ttacctatcg ttttttcatc cagaataaca tcgtctcttc ggtatggctg
    65881 cactggcagt attgcatacc cacgaaaatc agtactcagt cccggccagc cattaacgtt
    65941 aacccccgtt gctccgggtg cctctataag ggctaatgtc ccatccacat tttgaccaaa
    66001 tgtaagtcca tcccttgtaa taagtatccc accgcttaaa tttgcaccta gctgtttcat
    66061 gttttcgctg gagctgtaat ggctactaaa ctgaccatat gatccatttt tgccaacttg
    66121 cacaatgttc atcgtgctct tcggttgtga ctgatgagcg ttagttatat ttgtcgctac
    66181 atcccaccat acaccaccac tattctgacc tgataatgaa acctgatagt tattaccctg
    66241 ctctcccgaa cgactcatct gtgaagacgc ataaatttga ttttcgtcac ccagcgataa
    66301 tggcactgaa atacccacgc ttaatttccg atctactttc catttatgcg atgtcagcaa
    66361 gtcgtttttt gcccagttaa tggagaatgt tatgcctctc cataccgtac tataactcag
    66421 gctggtagac cgcgtgtttc ctgtgccccg ggaagaatta tacctcgtgg tattgaagtt
    66481 caaattcccg atattaccaa ggggctggga taaggatatc gtagagccat cgtgactatt
    66541 cttatctctt tttctgttcc gttcatcctg tgcacttact gcttcagtca gtgtggcata
    66601 acctgatgca taacggttcg tggttataga
//
LOCUS       DAJTUM010000010        66023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTUM010000010 DAJTUM010000000
VERSION     DAJTUM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OO471_000715"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OO471_000715"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6242095.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO471_000716"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO471_000716"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6242096.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO471_000717"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO471_000717"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6242097.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO471_000718"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO471_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6242098.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO471_000719"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO471_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6242099.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO471_000720"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO471_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6242100.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OO471_000721"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OO471_000721"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6242101.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="OO471_000722"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="OO471_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6242102.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="OO471_000723"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="OO471_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6242103.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="OO471_000724"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="OO471_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6242104.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="OO471_000725"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="OO471_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6242105.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="OO471_000726"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="OO471_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6242106.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OO471_000727"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OO471_000727"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6242107.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="OO471_000728"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="OO471_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6242108.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="OO471_000729"
     CDS             19305..19370
                     /locus_tag="OO471_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242109.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="OO471_000730"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="OO471_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6242110.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="OO471_000731"
     CDS             complement(19754..20377)
                     /locus_tag="OO471_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6242111.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OO471_000732"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OO471_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6242112.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OO471_000733"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OO471_000733"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6242113.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="OO471_000734"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="OO471_000734"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6242114.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="OO471_000735"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="OO471_000735"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6242115.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /gene="cdaR"
                     /locus_tag="OO471_000736"
                     /pseudo
     CDS             25216..26372
                     /gene="cdaR"
                     /locus_tag="OO471_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26461..26847)
                     /locus_tag="OO471_000737"
     CDS             complement(26461..26847)
                     /locus_tag="OO471_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6242116.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OO471_000738"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OO471_000738"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6242117.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OO471_000739"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OO471_000739"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6242118.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="OO471_000740"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="OO471_000740"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6242119.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="OO471_000741"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="OO471_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6242120.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="OO471_000742"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="OO471_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6242121.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="OO471_000743"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="OO471_000743"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6242122.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="OO471_000744"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="OO471_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6242123.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="OO471_000745"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="OO471_000745"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6242124.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="OO471_000746"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="OO471_000746"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6242125.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="OO471_000747"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="OO471_000747"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6242126.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="OO471_000748"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="OO471_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6242127.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="OO471_000749"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="OO471_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6242128.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="OO471_000750"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="OO471_000750"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6242129.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="OO471_000751"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="OO471_000751"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6242130.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="OO471_000752"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="OO471_000752"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6242131.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="OO471_000753"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="OO471_000753"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6242132.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="OO471_000754"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="OO471_000754"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6242133.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="OO471_000755"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="OO471_000755"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6242134.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="OO471_000756"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="OO471_000756"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6242135.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="OO471_000757"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="OO471_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6242136.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..53858
                     /gene="ldcC"
                     /locus_tag="OO471_000758"
     CDS             51717..53858
                     /gene="ldcC"
                     /locus_tag="OO471_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6242137.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53915..54304
                     /locus_tag="OO471_000759"
     CDS             53915..54304
                     /locus_tag="OO471_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6242138.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54369..55667
                     /gene="tilS"
                     /locus_tag="OO471_000760"
     CDS             54369..55667
                     /gene="tilS"
                     /locus_tag="OO471_000760"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6242139.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OO471_000761"
     CDS             complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OO471_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6242140.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55963..56163)
                     /locus_tag="OO471_000762"
     CDS             complement(55963..56163)
                     /locus_tag="OO471_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6242141.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56329..56874
                     /locus_tag="OO471_000763"
     CDS             56329..56874
                     /locus_tag="OO471_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6242142.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56871..57293
                     /gene="arfB"
                     /locus_tag="OO471_000764"
     CDS             56871..57293
                     /gene="arfB"
                     /locus_tag="OO471_000764"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6242143.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57307..58017
                     /gene="nlpE"
                     /locus_tag="OO471_000765"
                     /gene_synonym="cutF"
     CDS             57307..58017
                     /gene="nlpE"
                     /locus_tag="OO471_000765"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6242144.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58172..58996)
                     /locus_tag="OO471_000766"
     CDS             complement(58172..58996)
                     /locus_tag="OO471_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6242145.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OO471_000767"
     CDS             complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OO471_000767"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6242146.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OO471_000768"
     CDS             complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OO471_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6242147.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OO471_000769"
     CDS             complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OO471_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6242148.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OO471_000770"
     CDS             complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OO471_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6242149.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OO471_000771"
     CDS             complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OO471_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6242150.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OO471_000772"
     CDS             complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OO471_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6242151.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64824..65399
                     /gene="gmhB"
                     /locus_tag="OO471_000773"
     CDS             64824..65399
                     /gene="gmhB"
                     /locus_tag="OO471_000773"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6242152.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65763..>66023
                     /locus_tag="OO471_000774"
     rRNA            65763..>66023
                     /locus_tag="OO471_000774"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15627 a        16342 c        18194 g        15860 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccacca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gggatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaaaataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acagcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagcg caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt attattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg gcagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgttcaa tctgtacaat ccggtgaatc ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccaataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc agcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcctgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gcttcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattccat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggg gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggggcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcggaccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgctaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccgtag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccgcttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaacac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagtccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgagtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttga cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga tcttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag caactgacta agatcgagcc gggcgagctg tataacggca
    40861 ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg ttattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgatggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52201 cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta
    52261 tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg gaagcggaag
    52321 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaatatcga
    52381 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52441 gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52501 tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc
    52561 gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg
    52621 tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc
    52681 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa
    52741 tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa
    52801 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag
    52861 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52921 gttatcccat tgttgcttcg gttaagacgg cggcggcgat gctgcgtggt aatccgggca
    52981 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc
    53041 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    53101 ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53161 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53221 gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg
    53281 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg
    53341 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc
    53401 tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc
    53461 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53521 tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53581 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53641 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53701 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53761 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53821 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53881 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53941 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    54001 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    54061 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    54121 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54181 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54241 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54301 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54361 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54421 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54481 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54541 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54601 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcacgtt
    54661 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54721 acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54781 cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc
    54841 gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54901 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54961 tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca ctttgtgctg
    55021 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    55081 aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta
    55141 ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca
    55201 tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct
    55261 ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55321 aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55381 ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55441 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga
    55501 agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55561 tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55621 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55681 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta
    55741 ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca
    55801 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc
    55861 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55921 tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc
    55981 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    56041 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    56101 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    56161 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56221 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56281 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56341 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56401 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56461 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56521 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56581 gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctg
    56641 tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg
    56701 cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc
    56761 tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat ctggttatcg
    56821 gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga
    56881 tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg
    56941 gcgctggcgg gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc
    57001 gggcgtccag cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga
    57061 tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc
    57121 gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa gaacaaaaag
    57181 cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac
    57241 aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag
    57301 gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg
    57361 atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg
    57421 aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga aggaatcgaa
    57481 atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct
    57541 cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg
    57601 gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag
    57661 atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct ggaaccggca
    57721 caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta tatggctgat
    57781 gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag
    57841 ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta
    57901 gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa agtattagcg
    57961 cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg gcagtaaacc
    58021 tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca ggcctacagc
    58081 atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc
    58141 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
    58201 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58261 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58321 gcgccagtgt aagtggatgc ccggatttgg caaatgcatc agtgacaaat tccgaacaaa
    58381 accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc aggtcgctga
    58441 cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
    58501 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    58561 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    58621 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    58681 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    58741 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    58801 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    58861 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
    58921 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg cggcagtacg
    58981 cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat tcaggaccct
    59041 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
    59101 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
    59161 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
    59221 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
    59281 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
    59341 acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta gttctgctca
    59401 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
    59461 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
    59521 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
    59581 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
    59641 tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
    59701 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
    59761 cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
    59821 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
    59881 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
    59941 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
    60001 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
    60061 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
    60121 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggcc
    60181 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
    60241 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
    60301 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
    60361 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
    60421 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
    60481 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcggct
    60541 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
    60601 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
    60661 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
    60721 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
    60781 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga tttagtttac
    60841 cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc agcgcaaaga
    60901 cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc cagacggcat
    60961 aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc gccagtactt
    61021 cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc tgcttttcga
    61081 cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca tagctggcac
    61141 tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact accggcgtac
    61201 catcgaccag atccagactg ccgagcttca gaatcacgct gcctttatgg caaacaacct
    61261 ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag cgtgttgcga
    61321 aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga cgccagccgc
    61381 cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct tccaggccgc
    61441 gaacggcgtc ggcctggttg taggaagcta tgagatgcag ttcgccgttg gcgcttttta
    61501 ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga ataacgccta
    61561 tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc cgatacatac
    61621 agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca gtaatacagc
    61681 attggctttc attttagagg cattgatttg catctgctta cgtgcggttg gaatgctcgg
    61741 cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga gatcgcggaa
    61801 cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc
    61861 tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg
    61921 ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga tcggtaaagc
    61981 acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg agcttgtaaa
    62041 aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga aacagcctaa
    62101 aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt cgtaaacttc
    62161 atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt tatcttcacg
    62221 cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac cgtctttcgc
    62281 cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag cgatttgcgc
    62341 gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca gattttttgg
    62401 gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca gtttgatcaa
    62461 gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt ttggcacggc
    62521 aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt aaccagcaat
    62581 cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt tcagttgctg
    62641 atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc
    62701 gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat atttgtcttt
    62761 cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca cgccgacttt
    62821 aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg atccaatcag
    62881 ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct tcttcaatta
    62941 tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt cgcctgcaaa
    63001 ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt
    63061 gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac cgactgcacc
    63121 acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat tcaccagacc
    63181 cggcaacgct tccggtaaca gcactttaca gacgatctgc attggcgtgg cacccattgc
    63241 gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga ccatacgggc
    63301 aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac caatcgatgt
    63361 accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga acgggatgga
    63421 acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag caataatttg
    63481 ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa acccaaaaaa
    63541 tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca gccacatcat
    63601 cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc caggcaatgg
    63661 cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg aatttaacgc
    63721 caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg
    63781 tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc agcatcggca
    63841 cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg atatccagat
    63901 gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag aacacttcac
    63961 ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa tcacaaatac
    64021 gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga cggcggttaa
    64081 tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg ctggtggctt
    64141 catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc acacgttgtt
    64201 tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga ccaaccaatg
    64261 acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc agctccagcg
    64321 gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc tggaaaatca
    64381 taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc aattcctggc
    64441 catcgaccag cacgctaccc tcggttgggc gctccagcaa gtttacacaa cgtataagcg
    64501 tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct ggcacatgca
    64561 ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact ttggtgatat
    64621 tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag catctcgaat
    64681 gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac gtctaaatca
    64741 atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact agcgtcccat
    64801 gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt cttgaccgtg
    64861 atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt gaatttatcg
    64921 acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa
    64981 ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa acgctgaccg
    65041 agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc tattattgcc
    65101 cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc cgcaaaccac
    65161 atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc gcttcttata
    65221 tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg ggaacaaaag
    65281 tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca gattgggtgt
    65341 taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg gcgcaatgat
    65401 taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg agccgactcc
    65461 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg
    65521 gttgaagaga aaaaatcctg aaattcaggg ttgactctga aataggaaag tgtaatatac
    65581 gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca atttatcaga
    65641 caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacgat aaatgaatac
    65701 caagtctcaa gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt
    65761 ttaaattgaa gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc
    65821 aagtcgaacg gtaacaggaa gcgcttgctg cttcgctgac gagtggcgga cgggtgagta
    65881 atgtctggga aactgcctga tggaggggga taactactgg aaacggtagc taataccgca
    65941 taacgtcgca agaccaaaga gggggacctt cgggcctctt gccatcggat gtgcccagat
    66001 gggattagct tgttggtggg gta
//
LOCUS       DAJTUM010000011        64184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTUM010000011 DAJTUM010000000
VERSION     DAJTUM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            121..564
                     /locus_tag="OO471_000775"
     CDS             121..564
                     /locus_tag="OO471_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6242153.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            800..1618
                     /gene="ybhA"
                     /locus_tag="OO471_000776"
     CDS             800..1618
                     /gene="ybhA"
                     /locus_tag="OO471_000776"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6242154.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO471_000777"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO471_000777"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6242155.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO471_000778"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO471_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6242156.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO471_000779"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO471_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6242157.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO471_000780"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO471_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6242158.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO471_000781"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO471_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6242159.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO471_000782"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO471_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6242160.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO471_000783"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO471_000783"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6242161.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO471_000784"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO471_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6242162.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO471_000785"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO471_000785"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6242163.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO471_000786"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO471_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6242164.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO471_000787"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO471_000787"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6242165.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO471_000788"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO471_000788"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6242166.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLSAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO471_000789"
     CDS             13924..14304
                     /locus_tag="OO471_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6242167.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO471_000790"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO471_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6242168.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO471_000791"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO471_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6242169.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO471_000792"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO471_000792"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6242170.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO471_000793"
     tRNA            complement(17405..17480)
                     /locus_tag="OO471_000793"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO471_000794"
     tRNA            complement(17484..17559)
                     /locus_tag="OO471_000794"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO471_000795"
     tRNA            complement(17611..17686)
                     /locus_tag="OO471_000795"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO471_000796"
     tRNA            complement(17689..17764)
                     /locus_tag="OO471_000796"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17900..17975)
                     /locus_tag="OO471_000797"
     tRNA            complement(17900..17975)
                     /locus_tag="OO471_000797"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17940..17942),aa:Lys,seq:ttt)
     gene            complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO471_000798"
     CDS             complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO471_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6242171.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO471_000799"
     CDS             complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO471_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6242172.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO471_000800"
     CDS             complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO471_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6242173.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO471_000801"
     CDS             complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO471_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6242174.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO471_000802"
     CDS             complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO471_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6242175.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO471_000803"
     CDS             complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO471_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6242176.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO471_000804"
     CDS             complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO471_000804"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6242177.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO471_000805"
     CDS             complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO471_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6242178.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO471_000806"
     CDS             complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO471_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6242179.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO471_000807"
     CDS             complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO471_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6242180.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO471_000808"
     CDS             complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO471_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6242181.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO471_000809"
     CDS             complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO471_000809"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6242182.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO471_000810"
     CDS             complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO471_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6242183.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32633..33355
                     /gene="mngR"
                     /locus_tag="OO471_000811"
     CDS             32633..33355
                     /gene="mngR"
                     /locus_tag="OO471_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6242184.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO471_000812"
     CDS             complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO471_000812"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6242185.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO471_000813"
     CDS             complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO471_000813"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6242186.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO471_000814"
     CDS             complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO471_000814"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6242187.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO471_000815"
     CDS             complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO471_000815"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6242188.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO471_000816"
     CDS             complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO471_000816"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6242189.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO471_000817"
     CDS             complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO471_000817"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6242190.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO471_000818"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO471_000818"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6242191.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO471_000819"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO471_000819"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6242192.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43545..43652
                     /locus_tag="OO471_000820"
     CDS             43545..43652
                     /locus_tag="OO471_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242193.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43858..45141
                     /gene="gltA"
                     /locus_tag="OO471_000821"
     CDS             43858..45141
                     /gene="gltA"
                     /locus_tag="OO471_000821"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6242194.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45532..46098
                     /locus_tag="OO471_000822"
     CDS             45532..46098
                     /locus_tag="OO471_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6242195.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46149..48608
                     /locus_tag="OO471_000823"
     CDS             46149..48608
                     /locus_tag="OO471_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6242196.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48623..49354
                     /locus_tag="OO471_000824"
     CDS             48623..49354
                     /locus_tag="OO471_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6242197.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49351..50427
                     /locus_tag="OO471_000825"
                     /pseudo
     CDS             49351..50427
                     /locus_tag="OO471_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50579..51625
                     /locus_tag="OO471_000826"
     CDS             50579..51625
                     /locus_tag="OO471_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6242198.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO471_000827"
     CDS             complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO471_000827"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6242199.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO471_000828"
     CDS             complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO471_000828"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6242200.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO471_000829"
                     /pseudo
     CDS             complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO471_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO471_000830"
     CDS             complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO471_000830"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6242201.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO471_000831"
     CDS             complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO471_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6242202.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55792..57273
                     /gene="dtpD"
                     /locus_tag="OO471_000832"
     CDS             55792..57273
                     /gene="dtpD"
                     /locus_tag="OO471_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6242203.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO471_000833"
     CDS             complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO471_000833"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6242204.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58838..59347)
                     /locus_tag="OO471_000834"
     CDS             complement(58838..59347)
                     /locus_tag="OO471_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6242205.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59476..59763
                     /locus_tag="OO471_000835"
     CDS             59476..59763
                     /locus_tag="OO471_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6242206.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59818..60198)
                     /locus_tag="OO471_000836"
     CDS             complement(59818..60198)
                     /locus_tag="OO471_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242207.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60201..63587)
                     /locus_tag="OO471_000837"
     CDS             complement(60201..63587)
                     /locus_tag="OO471_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6242208.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63606..63809)
                     /locus_tag="OO471_000838"
                     /pseudo
     CDS             complement(63606..63809)
                     /locus_tag="OO471_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15691 a        17276 c        15617 g        15600 t
ORIGIN      
        1 gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt
       61 gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc
      121 atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag
      181 gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt
      241 tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg
      301 aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca
      361 gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca
      421 tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc
      481 actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga
      541 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg ccacttgtaa
      601 ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca
      661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      841 attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga
      901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
     1021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag ctgacagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa atgcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg caggatgact
    17821 cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca cgttttacta
    17881 ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    17941 aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt
    18001 tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat
    18061 ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact
    18121 tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat
    18181 cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac
    18241 ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg
    18301 gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt
    18361 agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt
    18421 tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg
    18481 caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg ccagcattcg
    18541 cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga tcgctgctgg
    18601 ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt
    18661 gccgctccac gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat
    18721 caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag
    18781 cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ctgacactac
    18841 tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac
    18901 tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac
    18961 gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt
    19021 aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg
    19081 cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt
    19141 gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca
    19201 gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt
    19261 tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc
    19321 cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg
    19381 cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt
    19441 tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac
    19501 agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg
    19561 aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag
    19621 attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga
    19681 acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag
    19741 ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct
    19801 tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga
    19861 ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta
    19921 ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac
    19981 aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat
    20041 gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc
    20101 gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg
    20161 tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg
    20221 taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca
    20281 acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca
    20341 tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga
    20401 gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa
    20461 ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag tgcggaccat
    20521 gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac
    20581 ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt
    20641 gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca
    20701 ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa
    20761 ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac
    20821 gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa
    20881 ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat
    20941 ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt
    21001 ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc
    21061 agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac
    21121 gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata gttattgata
    21181 tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc
    21241 cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata
    21301 tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgccgct
    21361 gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgcctta
    21421 tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc
    21481 ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt ggctttttca
    21541 gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct
    21601 ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc tgcggctttg
    21661 gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc tgcttcttcc
    21721 gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt ctgctttaac
    21781 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    21841 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    21901 gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga ttcctggctt
    21961 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    22021 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    22081 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    22141 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    22201 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    22261 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    22321 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    22381 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    22441 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    22501 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    22561 aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga tttcggactt
    22621 gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct
    22681 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    22741 cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc
    22801 ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    22861 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    22921 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    22981 ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac
    23041 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct
    23101 gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag
    23161 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cacgcgccgc
    23221 tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg aaaaaccaat
    23281 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    23341 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    23401 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg
    23461 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    23521 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    23581 acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc
    23641 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    23701 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    23761 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    23821 gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga gcggcccata
    23881 attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    23941 tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca
    24001 cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca
    24061 gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa
    24121 gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg
    24181 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag
    24241 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    24301 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    24361 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    24421 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    24481 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    24541 ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg
    24601 gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc cagtgccgga
    24661 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    24721 acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    24781 ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc
    24841 gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    24901 agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg
    24961 aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    25021 ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg
    25081 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    25141 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc
    25201 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg
    25261 cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata
    25321 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    25381 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    25441 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    25501 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    25561 aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    25621 accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc tgtcggcagc
    25681 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    25741 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca
    25801 atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag caggaagcca
    25861 cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg
    25921 gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    25981 agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgtctggtcg
    26041 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    26101 tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt
    26161 gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga
    26221 tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca
    26281 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    26341 acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta
    26401 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    26461 gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta
    26521 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    26581 gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa
    26641 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    26701 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    26761 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    26821 tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg
    26881 ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc
    26941 ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    27001 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    27061 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    27121 agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga
    27181 aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt acgccgcaaa
    27241 atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt
    27301 tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgatg
    27361 cccatttcca agacttatac attgataaat atcaatttct acaaattatg ttaatcatgt
    27421 ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata agactgaaat
    27481 tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac ggttgacctt
    27541 aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt aataaaggca
    27601 taaatctccc ccccgccgat attaacattt aattaactta taattatatt ataattaact
    27661 cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg agagtgaaga
    27721 taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
    27781 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
    27841 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
    27901 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
    27961 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
    28021 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
    28081 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat cccactgggg
    28141 gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg agcttcatcg
    28201 catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc gcctgctgag
    28261 ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga caagaaagca
    28321 gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc ctaggcctta
    28381 aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg gcaaaggttt
    28441 tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca gccatgccgt
    28501 tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc ggcgcttctt
    28561 tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc gttagcgaac
    28621 caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc aggacgcgaa
    28681 ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc ctgttgttat
    28741 gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt gcgctgcgtt
    28801 gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc tgccaggcct
    28861 catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac tcttcttttg
    28921 cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc aacacccggt
    28981 catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca ttgagcgcaa
    29041 tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca atcacgttgc
    29101 caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca atctggttga
    29161 tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt tgccgatcga
    29221 ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc tgaccgcgat
    29281 cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc acttcttccc
    29341 gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc ggcatgttgt
    29401 cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca gccagttcga
    29461 agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg ctgtcgtggg
    29521 catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg tatccaaaac
    29581 ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta ttttcaatac
    29641 gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat
    29701 atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct
    29761 tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca
    29821 gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca
    29881 gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt
    29941 tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca
    30001 cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac
    30061 cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga
    30121 tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct
    30181 cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag
    30241 aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta
    30301 ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg
    30361 ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga
    30421 tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc
    30481 gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct
    30541 ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg
    30601 ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc
    30661 aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc
    30721 agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga
    30781 gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc
    30841 ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc
    30901 tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt
    30961 gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa
    31021 tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat
    31081 ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg taagcgagtt
    31141 attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt
    31201 accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa
    31261 gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag
    31321 aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg
    31381 tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag
    31441 cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc
    31501 ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac
    31561 aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac
    31621 gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac gctcgagggt
    31681 aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcacagaaag
    31741 cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca
    31801 ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg
    31861 cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc
    31921 catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt
    31981 ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc
    32041 aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag
    32101 cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg ccagcaacac
    32161 cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct cgctgagtgt
    32221 ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc
    32281 ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc
    32341 agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc
    32401 acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcaggtt
    32461 catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac aaaacaatac
    32521 cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta ttgtttacag
    32581 gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca
    32641 caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct
    32701 taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg
    32761 ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca
    32821 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    32881 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga
    32941 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt
    33001 ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    33061 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    33121 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    33181 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    33241 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    33301 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    33361 aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta
    33421 gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg
    33481 cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga
    33541 atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt
    33601 tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta acatgctctt
    33661 tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg
    33721 cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta gagccaggga
    33781 tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc gtggtctgtt
    33841 taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta cccggtttgt
    33901 gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg cctgggcagt
    33961 tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc agcatatcca
    34021 gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg atagcttcca
    34081 gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa gcgccagtag
    34141 cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta gtgccgcctt
    34201 tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt tctgagtgga
    34261 aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta tcaattaaaa
    34321 tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt
    34381 tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt
    34441 accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg cgccgatgat
    34501 accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt
    34561 cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc
    34621 gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta tgtccatcgt
    34681 acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta
    34741 gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat
    34801 ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt tgccgtcgag
    34861 gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca gcgccaggtc
    34921 gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt
    34981 accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag
    35041 cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt tttcgatttc
    35101 cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt ctacaacggc
    35161 accgagatac agctctttag cgatatcggt cgccgcttca accagaatct ggttaaccgg
    35221 ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt tttctgcaaa
    35281 agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc
    35341 agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag cggcttcttc
    35401 tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa
    35461 cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt
    35521 tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt
    35581 aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt tgatcgttac
    35641 caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta
    35701 catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg
    35761 catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca gggaaccgaa
    35821 cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg tcagcttgcc
    35881 atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca tgccgagggt
    35941 atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg ccatgctgac
    36001 gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca cttccgggta
    36061 acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc
    36121 gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt
    36181 gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc
    36241 cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag ccagagccgg
    36301 ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt tcgccagatg
    36361 tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa tggcgctggc
    36421 gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg
    36481 ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc
    36541 gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt
    36601 taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac
    36661 ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc
    36721 gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg
    36781 gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca
    36841 ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg
    36901 gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc acggaaatga
    36961 tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc
    37021 ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt
    37081 tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg ttccagcagg
    37141 tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg
    37201 tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggaaacc
    37261 gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg acgcatcccg
    37321 cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg tacgcaaacc
    37381 tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc
    37441 ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat ccggccccat
    37501 ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca
    37561 ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata accatattca
    37621 aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc gccctgaccg
    37681 ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg
    37741 tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct
    37801 tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca
    37861 ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact
    37921 gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat ctcaactttg
    37981 ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg
    38041 ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc gcggtacagg
    38101 ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag cttgtcagcg
    38161 tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg ctgggttgcg
    38221 ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc gatgaagaca
    38281 tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc
    38341 ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat atcagtacag
    38401 tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg gttgttgata
    38461 acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagagtttcc
    38521 tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg cagcactttg
    38581 ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat aacaaccggg
    38641 cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg
    38701 gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc gaggtgttct
    38761 ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc
    38821 agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg
    38881 ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc ttccagcgag
    38941 aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc agcggcggtc
    39001 agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata
    39061 cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc ggcaccaatc
    39121 gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat ggtttctttg
    39181 ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt cagatcgtgg
    39241 aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg atctagattc
    39301 gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc
    39361 acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg
    39421 cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac
    39481 tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca
    39541 tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct
    39601 ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt
    39661 ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt
    39721 agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt
    39781 tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg gtaatgactt
    39841 gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc aacgcttgcg
    39901 cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt gatatggccg
    39961 atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga
    40021 cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca
    40081 cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg
    40141 ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac
    40201 agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt
    40261 tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg
    40321 tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg
    40381 cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg
    40441 ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga caggctggga
    40501 tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct
    40561 tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt
    40621 gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat
    40681 gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactcaggc
    40741 agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta
    40801 tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc
    40861 atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca
    40921 tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag ccctttcgcc
    40981 atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg cagcgcttta
    41041 cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag gcgatccaga
    41101 gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc gatagactct
    41161 tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga gttgccgccc
    41221 agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa cagtcccgga
    41281 acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac tttggtcgga
    41341 ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatcg
    41401 acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc gagaacttct
    41461 ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga
    41521 ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc caggtctttg
    41581 gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct
    41641 tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt cgggtggaat
    41701 tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc gacaccgtcg
    41761 ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc gccagtcgcc
    41821 agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca cagtgcggta
    41881 caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa
    41941 atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc gtgaccggta
    42001 cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg
    42061 ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag gcccatgtgt
    42121 tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca
    42181 ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg
    42241 gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg
    42301 aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc
    42361 atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg caatttcatc
    42421 acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca acgacaatca
    42481 ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac acctgccaca
    42541 tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac actttggtga
    42601 acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg gtggcgaaaa
    42661 aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga
    42721 aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc
    42781 gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt tacccgcttc
    42841 gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg
    42901 atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat
    42961 cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa gccactgcag
    43021 gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc
    43081 cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt
    43141 tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact acgggcacag
    43201 aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt
    43261 cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac
    43321 gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt
    43381 ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg
    43441 aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa
    43501 tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt aaaagttatg
    43561 aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat
    43621 tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg
    43681 ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct gtacccaggt
    43741 tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg
    43801 cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg
    43861 gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact ggatgtgctg
    43921 aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtgttc
    43981 acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat
    44041 ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct
    44101 aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat
    44161 gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg
    44221 ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat taccggcgcg
    44281 ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc
    44341 gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt
    44401 ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt cctgaatatg
    44461 atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac
    44521 cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc
    44581 gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg
    44641 ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aattagctcc
    44701 gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg
    44761 atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa
    44821 acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga agtggctatg
    44881 gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact gtacccgaac
    44941 gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc catgttcacc
    45001 gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga aatgcacagt
    45061 tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt
    45121 aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta acccgccgtt
    45181 caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat aaaatccact
    45241 atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa caaatagatg
    45301 atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata tagcgcaccc
    45361 ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat taattacttt
    45421 tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata
    45481 agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa
    45541 ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg
    45601 gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa
    45661 atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt
    45721 aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa ctgtgatctg
    45781 gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc
    45841 tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc
    45901 ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact
    45961 ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg
    46021 gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac
    46081 gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt
    46141 tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg
    46201 cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa tcaatgcgcg
    46261 accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg
    46321 ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac tggcacaaaa
    46381 atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac
    46441 ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg
    46501 aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct
    46561 ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg
    46621 gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat ggcagcagca
    46681 gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg cgcttacgca
    46741 gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca ggcgaaatat
    46801 cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa
    46861 cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg
    46921 acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac
    46981 agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc
    47041 caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca
    47101 aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca tcgacgccct
    47161 tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca
    47221 tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt
    47281 caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat tctgccgcaa
    47341 tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca
    47401 gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca
    47461 aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc
    47521 attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg
    47581 aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac ctcaatcttt
    47641 acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc gccaacatca
    47701 cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct acgtcattca
    47761 atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg atttcgctcc
    47821 ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac agcaccacac
    47881 accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg tctgccggac
    47941 tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag cacctgagtt
    48001 cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt tccgtcagca
    48061 gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat cgccgcagct
    48121 ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt ccggttcagg
    48181 gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt tccagttacc
    48241 agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca gttgcagaaa
    48301 acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg gcttcccgtt
    48361 ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct cccctcggag
    48421 cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag gaaggacatg
    48481 cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt gatagccagc
    48541 attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg attttacctt
    48601 gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt tgttgttggt
    48661 tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg tgtttaacgc
    48721 taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc cctacctcgc
    48781 ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc
    48841 gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa agcaagcctc
    48901 gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc gtgaaattcc
    48961 tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat
    49021 caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg tcgaactgca
    49081 gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg cgtattatct
    49141 gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat
    49201 ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca gccagtttta
    49261 tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact gcagcggaca
    49321 atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc
    49381 atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta ctttggttca
    49441 tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc aggcaatgcc
    49501 aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa caaaatttgc
    49801 tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat gcggttatac
    49861 gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc
    49921 gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat
    49981 catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca
    50041 ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca
    50101 atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga
    50161 tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg
    50221 ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc
    50281 aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat
    50341 acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg
    50401 gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc
    50461 atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctag
    50521 cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat
    50581 gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct
    50641 cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag
    50701 tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct
    50761 tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg
    50821 gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt
    50881 attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg
    50941 cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt
    51001 tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat
    51061 gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat
    51121 taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg
    51181 tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca
    51241 gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg
    51301 gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca
    51361 aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac
    51421 ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga
    51481 taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca
    51541 aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac
    51601 ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga
    51661 ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg
    51721 aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt
    51781 agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc
    51841 attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg
    51901 caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg
    51961 gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc
    52021 cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa
    52081 catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac
    52141 ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta
    52201 aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca acgccttacc
    52261 acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg
    52321 cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc
    52381 cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc attctttatt
    52441 aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg
    52501 gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt
    52561 gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc
    52621 aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg
    52681 tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg
    52741 cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg
    52801 tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac
    52861 atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc
    52921 gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg
    52981 cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt
    53041 attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca
    53101 atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga
    53161 tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg
    53221 ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg
    53281 accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg caatacgtgg
    53341 ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgcggcac
    53401 ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga tgcgttttaa
    53461 aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct gccagggcga
    53521 acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc
    53581 gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt
    53641 cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca gcccgccaat
    53701 ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc
    53761 gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc
    53821 tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg cttcgcaacc
    53881 tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact gaccgagcgt
    53941 gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc
    54001 aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt gacgaccgcc
    54061 atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc ctccttctgc
    54121 ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca
    54181 aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa
    54241 acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca
    54301 gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga
    54361 aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg
    54421 ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc
    54481 accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt
    54541 tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata
    54601 ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt
    54661 cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc
    54721 gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta
    54781 tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc
    54841 gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat aaaaatgcaa
    54901 tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc
    54961 cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac
    55021 gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc
    55081 ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc cccacggcac
    55141 caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt
    55201 gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc
    55261 cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca
    55321 gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa
    55381 agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg
    55441 caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg
    55501 ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca
    55561 tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg
    55621 acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat
    55681 cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac
    55741 aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa
    55801 cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt
    55861 agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac
    55921 gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc
    55981 ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg
    56041 ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc
    56101 tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt
    56161 ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg
    56221 tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa
    56281 gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc gggtctggtc
    56341 attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg
    56401 cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg
    56461 cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg
    56521 actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag
    56581 cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc
    56641 gcacaacagg gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg
    56701 tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg
    56761 tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc
    56821 atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc
    56881 ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc
    56941 ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg
    57001 cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc
    57061 gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat
    57121 gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc
    57181 tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa
    57241 ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac
    57301 gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt
    57361 gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc
    57421 tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg
    57481 tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac
    57541 ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg acgaataaac
    57601 tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg aaaatacggc
    57661 gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca
    57721 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct
    57781 ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc agtgctgtta
    57841 agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg ccaggcctgt
    57901 aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa tggacgatgt
    57961 ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca
    58021 ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga
    58081 tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa acggctggtg
    58141 ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt ctcgcaaaac
    58201 tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac
    58261 tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct actacgaact
    58321 tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
    58381 aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc cggcggcagg
    58441 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacgtccaca
    58501 tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt aacgctgttt
    58561 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
    58621 aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg agcattgata
    58681 agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt agcgatatac
    58741 agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
    58801 agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct gaatggcgca
    58861 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat
    58921 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
    58981 gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa
    59041 aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa
    59101 gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt tgccactgat
    59161 gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg gccagaaaga
    59221 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
    59281 gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgta
    59341 gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt aaatgatgtt
    59401 tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc aagcaactaa
    59461 tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat ttccaaaaca
    59521 cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa aaccggtata
    59581 gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct gaaagaggcc
    59641 ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct gaccatcatc
    59701 gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct gaacgaaagt
    59761 taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc tgtcttatta
    59821 taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta catgaagagt
    59881 acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc caacataatc
    59941 atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata ttcgtagttt
    60001 aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta tcatagcata
    60061 ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa tttcaatcca
    60121 tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc cacttaataa
    60181 tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct aacacttcca
    60241 ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg tccatgacct
    60301 gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca atgatatgaa
    60361 cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct ggggcctgac
    60421 tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa ttgttcagcg
    60481 tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg attcgctttt
    60541 tggttactaa cagcacatgg agttaatcca agtggatcag catattttag aggattaggt
    60601 gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac catgaaccgc
    60661 ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc cctgtcgagg
    60721 tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc ctgtatcgtg
    60781 ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc cgtcatctct
    60841 tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc ctctttgccc
    60901 gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat gtacaggctg
    60961 cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa aaattttgtc
    61021 cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct ccccagtgca
    61081 tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg gtgttccgca
    61141 tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg gccaaattcg
    61201 tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac cacgctgttt
    61261 tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc gtccacctcc
    61321 gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc cagcgggtcg
    61381 tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc gccctgaggg
    61441 tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt cacctgtccg
    61501 gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata acgtgtgcgc
    61561 cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag ccggtactgt
    61621 tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg accttcatac
    61681 cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt catgcagtgg
    61741 ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac gccgttcagg
    61801 tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc agggaaatgc
    61861 tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag acggatgcac
    61921 ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta gtacagattc
    61981 agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg gtggtgcggg
    62041 tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg cccctgctca
    62101 ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg cagtacggct
    62161 tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc gtctgcgcta
    62221 cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc aaacattcct
    62281 tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa gtggcccggc
    62341 agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc ggcattgacc
    62401 ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac actggcagca
    62461 aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc cacggttgcc
    62521 accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa cggaccgcca
    62581 atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac cctggcctcg
    62641 caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac atcccgcgcg
    62701 ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag attactgatg
    62761 ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc cggattcagc
    62821 aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg tgcgagaaac
    62881 tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt cggggtggat
    62941 cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg tgtcacgcca
    63001 tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc ccgggacatc
    63061 agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat ttccagatat
    63121 tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc ttcctgttct
    63181 gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt cgccgctgcc
    63241 cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat gtaaccttca
    63301 atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac cttttctgtc
    63361 acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac ggccgtgcac
    63421 cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat gagggcatca
    63481 ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat aaacgacgta
    63541 tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc ccgttccctg
    63601 tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt tttaatttca
    63661 gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt gaaggccatc
    63721 atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg ctcgttaccc
    63781 cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc gtcataatgt
    63841 aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt taatcatgaa
    63901 tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat cactaataat
    63961 attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg tatttaacaa
    64021 tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac cgcctccata
    64081 taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat atcggatgcg
    64141 ttttaattat caactataaa tctataagct ttttattaac aaca
//
LOCUS       DAJTUM010000012        60419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTUM010000012 DAJTUM010000000
VERSION     DAJTUM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..322
                     /locus_tag="OO471_000839"
     CDS             <1..322
                     /locus_tag="OO471_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6242209.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO471_000840"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO471_000840"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6242210.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO471_000841"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO471_000841"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6242211.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO471_000842"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO471_000842"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6242212.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO471_000843"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO471_000843"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6242213.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO471_000844"
     CDS             complement(3726..4955)
                     /locus_tag="OO471_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6242214.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO471_000845"
     CDS             complement(5019..5459)
                     /locus_tag="OO471_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6242215.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO471_000846"
     CDS             complement(5564..6334)
                     /locus_tag="OO471_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6242216.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO471_000847"
     CDS             complement(6331..7257)
                     /locus_tag="OO471_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6242217.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO471_000848"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO471_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6242218.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO471_000849"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO471_000849"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6242219.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO471_000850"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO471_000850"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6242220.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPSNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO471_000851"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO471_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6242221.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO471_000852"
     CDS             12964..13311
                     /locus_tag="OO471_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6242222.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO471_000853"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO471_000853"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6242223.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO471_000854"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO471_000854"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6242224.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKARYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO471_000855"
     CDS             complement(15131..15493)
                     /locus_tag="OO471_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6242225.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO471_000856"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO471_000856"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6242226.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO471_000857"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO471_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO471_000858"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO471_000858"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6242227.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO471_000859"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO471_000859"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6242228.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO471_000860"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO471_000860"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6242229.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO471_000861"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO471_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6242230.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO471_000862"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO471_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6242231.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO471_000863"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO471_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6242232.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO471_000864"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO471_000864"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6242233.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO471_000865"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO471_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6242234.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO471_000866"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO471_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6242235.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO471_000867"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO471_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO471_000868"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO471_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6242236.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO471_000869"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO471_000869"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6242237.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO471_000870"
     CDS             complement(36348..36386)
                     /locus_tag="OO471_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242238.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO471_000871"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO471_000871"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6242239.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO471_000872"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO471_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6242240.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO471_000873"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO471_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6242241.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO471_000874"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO471_000874"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6242242.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO471_000875"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO471_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6242243.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO471_000876"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO471_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6242244.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO471_000877"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO471_000877"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6242245.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO471_000878"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO471_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6242246.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO471_000879"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO471_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6242247.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO471_000880"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO471_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6242248.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO471_000881"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO471_000881"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6242249.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO471_000882"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO471_000882"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6242250.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO471_000883"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO471_000883"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6242251.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO471_000884"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO471_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6242252.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO471_000885"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO471_000885"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6242253.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO471_000886"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO471_000886"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6242254.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO471_000887"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO471_000887"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6242255.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO471_000888"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO471_000888"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6242256.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO471_000889"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO471_000889"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6242257.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO471_000890"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO471_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6242258.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO471_000891"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO471_000891"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6242259.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO471_000892"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO471_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6242260.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16733 c        14850 g        14363 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgagtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgcggtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa cccatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAJTUM010000013        59874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTUM010000013 DAJTUM010000000
VERSION     DAJTUM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            545..1834
                     /gene="sdaC"
                     /locus_tag="OO471_000893"
     CDS             545..1834
                     /gene="sdaC"
                     /locus_tag="OO471_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6242261.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="OO471_000894"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="OO471_000894"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6242262.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3370..4125
                     /gene="xni"
                     /locus_tag="OO471_000895"
     CDS             3370..4125
                     /gene="xni"
                     /locus_tag="OO471_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6242263.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO471_000896"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO471_000896"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6242264.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO471_000897"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO471_000897"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6242265.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="OO471_000898"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="OO471_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6242266.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="OO471_000899"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="OO471_000899"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6242267.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEASACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="OO471_000900"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="OO471_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="OO471_000901"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="OO471_000901"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6242268.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="OO471_000902"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="OO471_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6242269.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO471_000903"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO471_000903"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6242270.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="OO471_000904"
     CDS             complement(13550..13945)
                     /locus_tag="OO471_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6242271.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO471_000905"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO471_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6242272.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="OO471_000906"
     CDS             complement(15232..15459)
                     /locus_tag="OO471_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6242273.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="OO471_000907"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="OO471_000907"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6242274.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="OO471_000908"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="OO471_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6242275.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="OO471_000909"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="OO471_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6242276.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18394..19491)
                     /gene="mltA"
                     /locus_tag="OO471_000910"
     CDS             complement(18394..19491)
                     /gene="mltA"
                     /locus_tag="OO471_000910"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6242277.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19700..19776
                     /locus_tag="OO471_000911"
     tRNA            19700..19776
                     /locus_tag="OO471_000911"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Met,seq:cat)
     gene            19810..19886
                     /locus_tag="OO471_000912"
     tRNA            19810..19886
                     /locus_tag="OO471_000912"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19844..19846,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="OO471_000913"
     CDS             complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="OO471_000913"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6242278.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="OO471_000914"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="OO471_000914"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6242279.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="OO471_000915"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="OO471_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6242280.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="OO471_000916"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="OO471_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6242281.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="OO471_000917"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="OO471_000917"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6242282.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="OO471_000918"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="OO471_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6242283.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34995)
                     /locus_tag="OO471_000919"
     CDS             complement(34645..34995)
                     /locus_tag="OO471_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6242284.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34980..35387)
                     /locus_tag="OO471_000920"
     CDS             complement(34980..35387)
                     /locus_tag="OO471_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6242285.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35384..35947)
                     /locus_tag="OO471_000921"
     CDS             complement(35384..35947)
                     /locus_tag="OO471_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6242286.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35938..36408)
                     /locus_tag="OO471_000922"
                     /pseudo
     CDS             complement(35938..36408)
                     /locus_tag="OO471_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36592..37386)
                     /gene="thyA"
                     /locus_tag="OO471_000923"
     CDS             complement(36592..37386)
                     /gene="thyA"
                     /locus_tag="OO471_000923"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6242287.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37393..38268)
                     /gene="lgt"
                     /locus_tag="OO471_000924"
     CDS             complement(37393..38268)
                     /gene="lgt"
                     /locus_tag="OO471_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6242288.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38419..40665)
                     /gene="ptsP"
                     /locus_tag="OO471_000925"
     CDS             complement(38419..40665)
                     /gene="ptsP"
                     /locus_tag="OO471_000925"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6242289.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40678..41208)
                     /gene="rppH"
                     /locus_tag="OO471_000926"
     CDS             complement(40678..41208)
                     /gene="rppH"
                     /locus_tag="OO471_000926"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6242290.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41893..42582
                     /gene="mutH"
                     /locus_tag="OO471_000927"
     CDS             41893..42582
                     /gene="mutH"
                     /locus_tag="OO471_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6242291.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42651..43364
                     /locus_tag="OO471_000928"
     CDS             42651..43364
                     /locus_tag="OO471_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6242292.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43502..43720
                     /gene="ygdR"
                     /locus_tag="OO471_000929"
     CDS             43502..43720
                     /gene="ygdR"
                     /locus_tag="OO471_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6242293.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43828..44868
                     /locus_tag="OO471_000930"
     CDS             43828..44868
                     /locus_tag="OO471_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6242294.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44901..46091)
                     /gene="lplT"
                     /locus_tag="OO471_000931"
     CDS             complement(44901..46091)
                     /gene="lplT"
                     /locus_tag="OO471_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6242295.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46084..48243)
                     /gene="aas"
                     /locus_tag="OO471_000932"
     CDS             complement(46084..48243)
                     /gene="aas"
                     /locus_tag="OO471_000932"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6242296.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48829..49860
                     /gene="galR"
                     /locus_tag="OO471_000933"
     CDS             48829..49860
                     /gene="galR"
                     /locus_tag="OO471_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6242297.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49867..51129)
                     /gene="lysA"
                     /locus_tag="OO471_000934"
     CDS             complement(49867..51129)
                     /gene="lysA"
                     /locus_tag="OO471_000934"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6242298.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51251..52186
                     /gene="lysR"
                     /locus_tag="OO471_000935"
     CDS             51251..52186
                     /gene="lysR"
                     /locus_tag="OO471_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6242299.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52173..52865)
                     /gene="ygeA"
                     /locus_tag="OO471_000936"
     CDS             complement(52173..52865)
                     /gene="ygeA"
                     /locus_tag="OO471_000936"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6242300.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52994..54412)
                     /gene="araE"
                     /locus_tag="OO471_000937"
     CDS             complement(52994..54412)
                     /gene="araE"
                     /locus_tag="OO471_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6242301.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54727..55488)
                     /gene="kduD"
                     /locus_tag="OO471_000938"
     CDS             complement(54727..55488)
                     /gene="kduD"
                     /locus_tag="OO471_000938"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6242302.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55518..56354)
                     /gene="kduI"
                     /locus_tag="OO471_000939"
     CDS             complement(55518..56354)
                     /gene="kduI"
                     /locus_tag="OO471_000939"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6242303.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56641..57822)
                     /locus_tag="OO471_000940"
     CDS             complement(56641..57822)
                     /locus_tag="OO471_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6242304.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58077..59306
                     /locus_tag="OO471_000941"
                     /pseudo
     CDS             58077..59306
                     /locus_tag="OO471_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14781 a        16065 c        14580 g        14448 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc
      121 atacaattta tgttgcaaca caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atcgccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacacaggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcaacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcctgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattctaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtttcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatgcc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 tctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg ctgcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttagt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccag cgaattcact actgatatca cgatggccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacaac gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatggaacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gattaaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt caaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccga tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaaacgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagagctgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg acggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcaa
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tgggattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcccctgt
    18361 catatccgta aaaacggcat acagaatatc acatcagccg ctaaagacgt tacctgcgcc
    18421 cggagccgtt ttcagcaccc aaacccgtcc atagtggttg taccaacctg cgcggtgtcc
    18481 ggcttccggc ccgatccctt gatagatatc gaagtgttgg cctttgattg cgcccccgac
    18541 atccaacgcc accatcagac gcaattcgta ctgaccgtta aatttgccgt tattatccag
    18601 cagcggcact tctgccagca aggtagtacc tggcggaata attgaacgat cggaggcaac
    18661 agaagcacgg ccaaccagcg gcaccgcact tgcccctttc accggtgcaa aagattgcgg
    18721 tttaaagaag acgaaagacg ggttctgttc cagcagctcg cgaacctcgg cttcactgtg
    18781 tgtttcgccc caatgacgaa tcgcctgcat cgacatatct tcttttttca cttcgccacg
    18841 gtcgatcagc accttaccaa tgctgcgata ggcatgaccg tttttccctg catagctgaa
    18901 aaagttaagc ggactgccat caccaaagtc gatataccca ctaccctgca catccataat
    18961 gaagttatcc atcagagagt tgctgtaagc gaggatgtat ttatcgccca atgccccggc
    19021 gtagatctcc gcacgagacg gcagacgacc acgttttggc ggcatacggt aaataggata
    19081 ctggaactcg ccctggcggg tatggcgcgc ctgaattacc ggcgtgtaat agcctgtaaa
    19141 ctgcacgtta ccatagttgt cggcaccttc catctgccag gcatcaatgc cgaactggcg
    19201 catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc accgcgttat aaacattact
    19261 ctggttgcca tacagacgcg gtgacgaatt acggatatgg ttaatttgct cgacaaaatc
    19321 accggcgtta atcggcgcgc caacggcatc tggctggttc accagagaga aaggctgggt
    19381 aaatttcccg tctttatatt gctgtccgcg atcggttggt ttggaagagc aggcggcaag
    19441 cattgccaca accgtgccca taagaaggta ctttacccaa cgtcctttca ttgttctctt
    19501 cttcaggtta aaaaataagg cgcaacgaag ataacaaacc gccgcgttta atgaaatgag
    19561 cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct ttagtggttt gtttttgttc
    19621 aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    19681 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    19741 cgaaggtcgt cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca
    19801 gtacggtgac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19861 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    19921 ctgatctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc ttcctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 aaataatcat gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgctcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgtt caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg attcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgtg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcatgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcaac agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaggcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcggttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaatc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcagatag attgcgcaat ttttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtat ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg agttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagcgcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acaattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gcttttggta ctggtttctg ctggcgagac tgttcattga
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcagcaccat
    34921 cagcaccatc agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt
    34981 cagggaagct gacataatgc cccctctttc agcggacaaa aatcgctcca gccgcgtggc
    35041 gaaaaaacaa tttttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa
    35101 ccggcaatca ataaggcttc attatctgcc agtaaacgca aacaaacctg ggcatcggtt
    35161 ccagcgtact gcgagcactg aactgctggc tgcgtctgcc aggagtgcat ttttccccac
    35221 gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttccat gctcacgcga
    35281 gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc
    35341 agcatcagga ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg
    35401 tcacgctata gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat
    35461 taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga
    35521 taatgatggc atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac
    35581 cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtcg gactctttta
    35641 ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac
    35701 cctgtccgac aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct
    35761 ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagt gccagcttac
    35821 gagtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca
    35881 atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta
    35941 caggcatcct tctgtttctc cttgctgata aagccggagt cttccccacg gcgaaaccac
    36001 cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg cggtattgcg
    36061 caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg
    36121 cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag
    36181 gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc
    36241 atcttcacgt aaatagagca aatagtcccg cgcctagctg gcggtttgcc aaagccgttg
    36301 cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag
    36361 catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg
    36421 gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta
    36481 tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag
    36541 ggtaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc
    36601 caccggcgct ttaacgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg
    36661 gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc
    36721 gcggcttaat tgcagatgag tttgatccat atggttgctg tacaggtgcg tatcgccacc
    36781 ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa
    36841 cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag
    36901 ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag
    36961 cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg
    37021 gtcgtttttc agctggttca gtaccgtagt aatctggtca atatgacgac catctggcgt
    37081 tggccaggcg cgccactgtt taccatacac tggcccgagg tcgccatttt catcggccca
    37141 ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtat cgccctgcag
    37201 gaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttggttg tcaccagcgg
    37261 gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc
    37321 ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg
    37381 tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat
    37441 gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac
    37501 ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc
    37561 gccgtagcca atcaggaaca agcctgagac agctcccatt gggcgcggtt tacgaatata
    37621 caggttgagg ataataaaca gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg
    37681 gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag
    37741 cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc
    37801 aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa
    37861 cggaatgagt ggtgcgataa aatcagagac ctggaagaag gaacgtttag tacggcgggc
    37921 gaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc
    37981 ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac
    38041 ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac
    38101 ttcatttttg gtccagccac tgcccggacg attcgcccga cgtgttgcca gccacattgc
    38161 aaaaatgaac cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga
    38221 gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa
    38281 gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg
    38341 ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag
    38401 atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc
    38461 cataaaggct gcaacctgat gacgaacttc ggtcgccagt tgcgcttcca gactacgctg
    38521 cgcaagattt tctgcttcgg caaaatcaat gcgccgcagc aggtatttta cccgcgctac
    38581 agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat
    38641 gggatcgccc gccatttcac cgcacaaacg aagatcgata ccatgtattt ccgcttcccg
    38701 ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac
    38761 ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga
    38821 gatgaaatcg accctctttg ccagatgcgg cagcataaat accattgacg gcacttccag
    38881 catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc
    38941 ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa
    39001 cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa
    39061 gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc
    39121 ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt
    39181 gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc
    39241 cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc
    39301 aatacagctg cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt
    39361 tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct
    39421 aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc
    39481 gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg
    39541 ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg
    39601 ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc
    39661 aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg
    39721 cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcacc
    39781 agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat
    39841 gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg
    39901 acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt
    39961 ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag
    40021 cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg
    40081 ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc
    40141 cggtaatgcg cggattcgcg tctggcgata ttgcccaaac agggcagtca actgcgactg
    40201 agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc
    40261 atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat
    40321 aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa
    40381 gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc
    40441 gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg
    40501 ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc
    40561 ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg
    40621 tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta
    40681 acctctttta cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga
    40741 catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac
    40801 cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact
    40861 gctggtttgc atattgattt ctgcatcgcc gcttaccagc tgcaagagaa accatttttg
    40921 tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta
    40981 gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc
    41041 ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg
    41101 cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt
    41161 acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca
    41221 aaataaagct ttatatacga atgattgttt catactccag gaagacggta aaccactctc
    41281 tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa
    41341 cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa
    41401 ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat agacatttta
    41461 aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg
    41521 tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat
    41581 ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg
    41641 tcaaggctgt aaatgaaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg
    41701 ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt
    41761 gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca
    41821 catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca
    41881 aggtatcatg acatgtccca acctcgccca ctgctctctc ctcccgaaac tgaagaacag
    41941 ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc ggcacttgac
    42001 gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag
    42061 atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg
    42121 gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt
    42181 gttgcccggt taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag
    42241 ctcaaacgcg tactgtggat accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt
    42301 cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa
    42361 gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt
    42421 cacggggaat atttacagat aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc
    42481 attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat
    42541 ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc
    42601 gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg
    42661 catggataac cgatcctaac gcttggcttg cgctcggtac gctgacgctg ctggagatcg
    42721 ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctt cccacagcac
    42781 aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc
    42841 tggcatcaat ctcttgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg
    42901 aaatttctgc ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca
    42961 gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat
    43021 cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg
    43081 tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggcagcc gtggtgattg
    43141 ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt
    43201 cggtaaaaat gctggcgctc tctttcctga ttctggtggg ctttaccctg attctggaaa
    43261 gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg
    43321 ttgaaagcct caacttgatt cgcaacaaaa agaatccgct ctgatactcc gtacgctctc
    43381 ctgcgggagg gcgtactcac tccttttacc ctcacagttt aagattttcc tgctttcaag
    43441 atatataacg tcggtttata aacagactat tatcataggt aggccaaatg aggataaccg
    43501 gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg
    43561 ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc
    43621 tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca
    43681 aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg
    43741 ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt atattcctta
    43801 tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca cagttcgctg
    43861 gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag cgaagccgac
    43921 gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga cgttgccgaa
    43981 atgtacccag tacctccgcg ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac
    44041 tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg
    44101 tcgcgcaata atgacaaggg catccgcccg gatcaggcgc tggatcggaa gaatatccgc
    44161 gaagcgctgc atgacagcct caagcgccta cagactgatt acctcgatct ttatcaggtg
    44221 cactggccgc agcgcccgac caactgcttc ggcaaactcg gttatagctg gacggattct
    44281 gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg cagagtacca acgcgcggga
    44341 aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat
    44401 ctggcggaca aacacgatct gccgcgtatt gtcaccattc agaaccctta cagtctgtta
    44461 aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaaggggt cgaactgctg
    44521 gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaatgg tgcaaaaccc
    44581 gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa
    44641 aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg gccttgatcc tgctcagatg
    44701 gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca ctctgctggg cgcaaccacg
    44761 atggatcagc tgaaaactaa catcgaaagt ttgcatctgg agttaagcga agacgtatta
    44821 gctgaaattg aagcggtaca tcaggtttat acttatcctg caccataaag attaacgccg
    44881 gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc cgttattgcc
    44941 agcgcaaata gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc taccattacc
    45001 gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag gttttgtact
    45061 gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa cgcattgagc
    45121 ggaacgacaa aaaagccccc cagcacgcca atcagcatca acaaggcata ggctggcagc
    45181 agctcgtgtt gcagggagaa aatcggtacc accacgccaa tcaaaatccc ggctggcata
    45241 cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata
    45301 ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc
    45361 accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca gaataaactg
    45421 gtgcccacca gcgaaaaacg cgtttcacca ttgcaccata gcgaggtgca ggcattcagg
    45481 aaactgcggg tcatgttgat gagattccag gactgccccg gacgcgccgc cagtttggga
    45541 atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc cagagcgacc
    45601 aggacatgcc agtcagccag cacaccaccg gctacggaac cgagtaaaat cgccgctatg
    45661 gcagaagctt ccattaaacc gttagctttt actaacttac tacccgtggt taattcgccg
    45721 agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag cgtatagccg
    45781 agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag gccgttggca
    45841 aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa
    45901 agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc cggatagaac
    45961 tgcgctttca gtaacgccag agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga
    46021 aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact
    46081 gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg
    46141 tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg gtacagcaag
    46201 ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca gttcgttatc
    46261 tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg ctcttaatcg cagtggcatg
    46321 gactttatct ggcgaaacac caagtgccag ttgttccacc atttccagcg acaccatttc
    46381 gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc
    46441 aaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc gaacattctc
    46501 ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt tcattatgtt
    46561 cggccctttc agttgcaggc gtccgccctc ttcgatacca gggaccgaca acaggcgtgc
    46621 atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg gtacgttgat
    46681 agaaacgaca ggcgcgcatt cggtcacacc gtagccttca aggatgcgca ggccaaattt
    46741 atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca ccacatagcg
    46801 tagacgatag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt
    46861 accgaacaac acggtgcaac tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag
    46921 cgggcttgga taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt
    46981 cagcccaaag gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc
    47041 ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt ttatggctat ggacgacgcc
    47101 ttttggatgg ccttcagaac cggaggtaaa gaggatcagc gcctcttctt ccggctgctg
    47161 ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag atccatactt tgtcggcagt
    47221 ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa gttgctccgg
    47281 cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc
    47341 agccgccgta atagcactgg tcagcccttt taccccggca gtgtagttca tcattgcggg
    47401 aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg cattgggcag
    47461 cattaagccg atgcgttcgc cttcaacact gtatttttca aggatgcgtc caacaaacag
    47521 cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt cgacacattt
    47581 cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagag tttcacgcgg
    47641 gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc atttcgccag cgattttgcg
    47701 acggtcacgg gcacgcggcg catccggcat cgccacctgc gttggaggca aaatatgcag
    47761 agtaatttgc gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtaag
    47821 ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaaacc
    47881 cgcgccatcg tagattttca tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat
    47941 caccactggt cggccctgtt ccaccagacg taccagatgt ttaatagcca taggttgcgt
    48001 cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct
    48061 tattgaggtg taaacggcaa acactggacg cacaggtaaa aacagtccaa gcaaaatgcc
    48121 atcaataaaa gagacgtgat taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt
    48181 gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag
    48241 cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata
    48301 aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg
    48361 tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat
    48421 cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga
    48481 aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa
    48541 acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acacgaagtt gacttcacct
    48601 atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat
    48661 cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta
    48721 tttgcgtttg gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt
    48781 ttccgtaaca ctgaaacaat gtaagcgttt acccactaag gtattttcat ggcgaccata
    48841 aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat
    48901 tcacccaaag ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc
    48961 tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg
    49021 atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg
    49081 gcttatcaca ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag
    49141 cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa
    49201 atgatcccag atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc
    49261 aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc
    49321 tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct
    49381 aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa
    49441 agtggtattc cggccaatga tcggctggtg acatttggcg aaccagacga aagcggcggc
    49501 gaacaggcaa tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat
    49561 aacgattcaa tggcggcggg cgcgatgggc gttctcaatg ataatggtat tgatgtaccg
    49621 ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc
    49681 ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc aggctgccga actggctttg
    49741 gcactggcgg ataatcgccc tctcccggaa attactaatg tatttagtcc gacgctggta
    49801 cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa
    49861 ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa
    49921 ccgcgcctga ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga
    49981 tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac
    50041 ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga
    50101 ttcacataac ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc
    50161 atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc
    50221 gttgaacccg gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac
    50281 ctgagtaatt aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt
    50341 cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata
    50401 atgttcggta tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc
    50461 agaaatagcc tgtaaatcct gaccgaattc gataacctga cgcaccatag caccacacac
    50521 ctgctccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc cgactagctg
    50581 cagatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg
    50641 cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt
    50701 aacgcgcagc catacccgat gccctggcga aacctggccc agttggtcga gcgtatcaac
    50761 agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat
    50821 aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa
    50881 cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa
    50941 gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccactt caaactgttt
    51001 cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa
    51061 ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga
    51121 atgtggcata acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga
    51181 ataaaaaata tcgatttatg tcgagtctat gcaaaaatga tatgaattac cggattgcga
    51241 gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac
    51301 cgccggaagc ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg
    51361 cgaactggcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg
    51421 attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg
    51481 actggatcgc attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc
    51541 tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct
    51601 ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga
    51661 gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata cgcctacggg
    51721 aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc
    51781 gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaatt acatcagcct
    51841 ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa
    51901 acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg
    51961 ggtaggtatt tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt
    52021 ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg
    52081 cccgtcatca gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact
    52141 ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc
    52201 tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt
    52261 aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca
    52321 atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca
    52381 aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa
    52441 aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc
    52501 agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt
    52561 aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta
    52621 cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg
    52681 tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg
    52741 ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt
    52801 atcagacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt
    52861 ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac
    52921 gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat
    52981 ccggcccgtg aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt
    53041 cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagta atacccacaa
    53101 acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca
    53161 gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg
    53221 taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg
    53281 gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg
    53341 acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag
    53401 tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta
    53461 ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa
    53521 tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca
    53581 tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag
    53641 cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc
    53701 tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca
    53761 acacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca
    53821 ggaagaccac cagaataatc agcagaactg ctggtaaagc aagaaccccc aacattgcgc
    53881 gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg
    53941 tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag
    54001 aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc accacacgag
    54061 cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca
    54121 ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc caaccattaa
    54181 acagcgcacc aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac
    54241 tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa
    54301 gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg
    54361 tatcacgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct
    54421 gccagcagag agtaagacat tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc
    54481 ggaaacagca taaatatgga ttaaattgct gcgacatgcc gttatgtgat ggatattcca
    54541 attttaaaat taagttgaat tattgagatt attattaacc tcctaatttt aaagcagata
    54601 aaattcataa agttcattaa ttgataattg atatggatta tttcataacc atgatatgga
    54661 ttatgatgat ctagagatat aaaaaaccct gccatgcggc agggtcataa aagtaagagg
    54721 aatgaattaa cgcgccagcc aaccgccatc cacagcaatg gtataaccat ttacataatc
    54781 tgaagcgctg gaggcaagaa acactaccgg ccccatcagg tcactcggca gtccccaacg
    54841 gccagctgga atgcgatcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg
    54901 ggtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
    54961 ccattcgttt gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga
    55021 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
    55081 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
    55141 attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg
    55201 aatcaatccg gcgttattca ccaggatatc aatatgacca aattcagcta ccgcgcgatc
    55261 cagcagtcca ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
    55321 ccccagcgcc gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat
    55381 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc
    55441 agtgacgacc gcaactttac cttggagaga aaatgcactt aaaatcataa caacacctta
    55501 ttcgtttatg cccacaacta gcgcaaatct ttaacgccca catggtccat atcattaaag
    55561 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
    55621 tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt
    55681 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
    55741 taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacctgga
    55801 gccagctccg tcagccccat actcaattga caggtttcca gtacatccgg gacaaaatat
    55861 ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
    55921 tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
    55981 ttcgccggag tggcggtatc aatactggca aagacaactt cttttgcacc tttaccaaca
    56041 tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca
    56101 ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt
    56161 ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata
    56221 attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc
    56281 aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc
    56341 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt
    56401 gaaacactgt ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat
    56461 taaaaataaa acaatgtttt atttttaatt taaggattaa aaaaagtgca gtgtcgatca
    56521 cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag
    56581 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag
    56641 ctactcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc
    56701 cagccctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca
    56761 accggaagcg cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg
    56821 ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
    56881 ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc
    56941 atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat
    57001 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc
    57061 agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc acagaaccga gactatcaaa
    57121 tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt
    57181 atcaacaacc aacgtctgac cgttactttg gatcattacc gggacgatct catctttaaa
    57241 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
    57301 caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
    57361 atcattgaag gcatcccaca acccatcatg caccagactg tcaaccaact ggctattgcc
    57421 aagctgttca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
    57481 gccacctgcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
    57541 tgctttaaga ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag
    57601 accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg
    57661 accaagaatt acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt
    57721 cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccac gaaagcagcc
    57781 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
    57841 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat
    57901 ttttatcaca aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt
    57961 tgcaaccact aacccaccaa tagatgggta gatagggcgt taatctccca tacttaactt
    58021 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
    58081 ctaatatttg gtctaaagaa gaaactctgt gaagtttcgc gctctatggc acagccgttg
    58141 atgcaggcac gctattcctt cctattcagt taggttcggc aggggctgtg gtcctgttta
    58201 ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca
    58261 tcctctcatc aaaaacatca gcaggtgaag ggataacggg cgcggtaaca cactactatg
    58321 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
    58381 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
    58441 ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaattctg aatctcattt
    58501 ttctgatggg acgtcatgcc actattcggg taatgggatt tttggtattc ccattgattg
    58561 cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacaa
    58621 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
    58681 ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
    58741 atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
    58801 gcataagtgt actgttcttt gtctttagct gcctgctttc tattccatct tcgtatattg
    58861 aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc
    58921 cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
    58981 tgggtacgta ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca acactacagc
    59041 aggttggtgt aaagaaaagt cgtgcattta accacgcact atcaattatg ttggtatcgc
    59101 tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca
    59161 gcggcccgct cattgccatg atacttttca tcatgcctac gctgtcaacg tatctcatcc
    59221 cggcgcttaa accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt
    59281 gcgtatcggt aatgttcttt agctaatatc cgttgtggat ggttcaaaat tgctacaatt
    59341 ttgaaccatt ccatttttca taatattcat ttgttattat tccgccctat cacttctctc
    59401 gtcatatcct agaattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa
    59461 gactatttaa gatgcataac ttagattcgc aagatatctt ctggtcattc aaaaacaatt
    59521 tgcgatatag atattctcat accatgagat agttatctgg accacgttcg accatgcaag
    59581 attaaatcag tgaaacataa tcatatttaa taacgagaaa tgcattttta aatatcttaa
    59641 tcagaattat agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcaag
    59701 gtgtattgac atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc
    59761 ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt
    59821 gccaaataat gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg tgtt
//
LOCUS       DAJTUM010000014        58030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTUM010000014 DAJTUM010000000
VERSION     DAJTUM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(24..278)
                     /locus_tag="OO471_000942"
     CDS             complement(24..278)
                     /locus_tag="OO471_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6242305.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO471_000943"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO471_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6242306.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO471_000944"
     CDS             1228..2694
                     /locus_tag="OO471_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6242307.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO471_000945"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO471_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6242308.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO471_000946"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO471_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6242309.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO471_000947"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO471_000947"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6242310.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO471_000948"
     CDS             5709..7265
                     /locus_tag="OO471_000948"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6242311.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO471_000949"
     CDS             7347..9161
                     /locus_tag="OO471_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6242312.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO471_000950"
     CDS             9162..10256
                     /locus_tag="OO471_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6242313.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO471_000951"
     CDS             10256..11281
                     /locus_tag="OO471_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6242314.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO471_000952"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO471_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6242315.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO471_000953"
     CDS             complement(12876..13220)
                     /locus_tag="OO471_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242316.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO471_000954"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO471_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6242317.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO471_000955"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO471_000955"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6242318.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO471_000956"
     CDS             15616..17376
                     /locus_tag="OO471_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6242319.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO471_000957"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO471_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6242320.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO471_000958"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO471_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6242321.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO471_000959"
     CDS             19113..19340
                     /locus_tag="OO471_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6242322.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO471_000960"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO471_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6242323.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO471_000961"
     tRNA            21195..21271
                     /locus_tag="OO471_000961"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO471_000962"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO471_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6242324.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO471_000963"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO471_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6242325.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO471_000964"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO471_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6242326.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWLQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO471_000965"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO471_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6242327.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO471_000966"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO471_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6242328.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO471_000967"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO471_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6242329.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO471_000968"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO471_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6242330.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO471_000969"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO471_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6242331.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO471_000970"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO471_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6242332.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO471_000971"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO471_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6242333.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO471_000972"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO471_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6242334.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO471_000973"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO471_000973"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6242335.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO471_000974"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO471_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6242336.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO471_000975"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO471_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6242337.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO471_000976"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO471_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6242338.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO471_000977"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO471_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6242339.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO471_000978"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO471_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6242340.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO471_000979"
     CDS             37391..37555
                     /locus_tag="OO471_000979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242341.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO471_000980"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO471_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6242342.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO471_000981"
     CDS             complement(38300..39331)
                     /locus_tag="OO471_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6242343.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO471_000982"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO471_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO471_000983"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO471_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6242344.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO471_000984"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO471_000984"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6242345.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO471_000985"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO471_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6242346.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO471_000986"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO471_000986"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6242347.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO471_000987"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO471_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6242348.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO471_000988"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO471_000988"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6242349.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO471_000989"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO471_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6242350.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO471_000990"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO471_000990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6242351.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO471_000991"
     CDS             complement(53338..54114)
                     /locus_tag="OO471_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6242352.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO471_000992"
     CDS             complement(54119..55768)
                     /locus_tag="OO471_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6242353.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO471_000993"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO471_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56487..>58030)
                     /locus_tag="OO471_000994"
     CDS             complement(56487..>58030)
                     /locus_tag="OO471_000994"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6242354.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14195 a        15655 c        14260 g        13920 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gcagccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta
//
LOCUS       DAJTUM010000015        55418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTUM010000015 DAJTUM010000000
VERSION     DAJTUM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO471_000995"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO471_000995"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6242355.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO471_000996"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO471_000996"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6242356.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO471_000997"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO471_000997"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6242357.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO471_000998"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO471_000998"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6242358.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO471_000999"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO471_000999"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6242359.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO471_001000"
     CDS             7826..9559
                     /locus_tag="OO471_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6242360.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO471_001001"
     CDS             9708..10559
                     /locus_tag="OO471_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6242361.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO471_001002"
     CDS             complement(10546..10887)
                     /locus_tag="OO471_001002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6242362.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO471_001003"
     CDS             complement(10889..11767)
                     /locus_tag="OO471_001003"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6242363.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO471_001004"
     CDS             complement(11733..14030)
                     /locus_tag="OO471_001004"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6242364.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO471_001005"
     CDS             complement(14081..14401)
                     /locus_tag="OO471_001005"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6242365.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO471_001006"
     CDS             complement(14416..15495)
                     /locus_tag="OO471_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6242366.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO471_001007"
     CDS             15804..16907
                     /locus_tag="OO471_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6242367.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO471_001008"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO471_001008"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6242368.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO471_001009"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO471_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO471_001010"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO471_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO471_001011"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO471_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO471_001012"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO471_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6242369.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO471_001013"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO471_001013"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6242370.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO471_001014"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO471_001014"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6242371.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO471_001015"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO471_001015"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6242372.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO471_001016"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO471_001016"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6242373.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO471_001017"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO471_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6242374.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO471_001018"
     CDS             30120..30728
                     /locus_tag="OO471_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6242375.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO471_001019"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO471_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6242376.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO471_001020"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO471_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6242377.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO471_001021"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO471_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6242378.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO471_001022"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO471_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6242379.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO471_001023"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO471_001023"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6242380.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO471_001024"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO471_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6242381.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO471_001025"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO471_001025"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6242382.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO471_001026"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO471_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6242383.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO471_001027"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO471_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6242384.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO471_001028"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO471_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO471_001029"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO471_001029"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6242385.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO471_001030"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO471_001030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6242386.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO471_001031"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO471_001031"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6242387.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO471_001032"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO471_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6242388.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO471_001033"
     CDS             complement(44843..45142)
                     /locus_tag="OO471_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6242389.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO471_001034"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO471_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO471_001035"
     CDS             45895..46494
                     /locus_tag="OO471_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6242390.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO471_001036"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO471_001036"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6242391.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO471_001037"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO471_001037"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6242392.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO471_001038"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO471_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6242393.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO471_001039"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO471_001039"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6242394.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO471_001040"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO471_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6242395.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO471_001041"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO471_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6242396.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO471_001042"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO471_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6242397.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO471_001043"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO471_001043"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6242398.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO471_001044"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO471_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6242399.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13491 a        15017 c        14166 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga gcgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaatacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAJTUM010000016        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTUM010000016 DAJTUM010000000
VERSION     DAJTUM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..273)
                     /locus_tag="OO471_001045"
     CDS             complement(<1..273)
                     /locus_tag="OO471_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6242400.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
     gene            complement(286..2067)
                     /gene="gcl"
                     /locus_tag="OO471_001046"
     CDS             complement(286..2067)
                     /gene="gcl"
                     /locus_tag="OO471_001046"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6242401.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2157..2972)
                     /gene="allR"
                     /locus_tag="OO471_001047"
     CDS             complement(2157..2972)
                     /gene="allR"
                     /locus_tag="OO471_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6242402.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3050..3532)
                     /gene="allA"
                     /locus_tag="OO471_001048"
     CDS             complement(3050..3532)
                     /gene="allA"
                     /locus_tag="OO471_001048"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6242403.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3762..4688
                     /gene="allS"
                     /locus_tag="OO471_001049"
     CDS             3762..4688
                     /gene="allS"
                     /locus_tag="OO471_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6242404.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            4757..5851
                     /gene="mnmH"
                     /locus_tag="OO471_001050"
     CDS             4757..5851
                     /gene="mnmH"
                     /locus_tag="OO471_001050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6242405.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(6564..8979)
                     /locus_tag="OO471_001051"
                     /pseudo
     CDS             complement(6564..8979)
                     /locus_tag="OO471_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(8976..9662)
                     /locus_tag="OO471_001052"
     CDS             complement(8976..9662)
                     /locus_tag="OO471_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6242406.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9633..10255
                     /gene="tesA"
                     /locus_tag="OO471_001053"
                     /pseudo
     CDS             9633..10255
                     /gene="tesA"
                     /locus_tag="OO471_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10245..11054
                     /gene="ybbO"
                     /locus_tag="OO471_001054"
     CDS             10245..11054
                     /gene="ybbO"
                     /locus_tag="OO471_001054"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6242407.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            11115..11969
                     /gene="cnoX"
                     /locus_tag="OO471_001055"
     CDS             11115..11969
                     /gene="cnoX"
                     /locus_tag="OO471_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6242408.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(12032..12811)
                     /gene="fetB"
                     /locus_tag="OO471_001056"
     CDS             complement(12032..12811)
                     /gene="fetB"
                     /locus_tag="OO471_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6242409.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12798..13475)
                     /gene="fetA"
                     /locus_tag="OO471_001057"
     CDS             complement(12798..13475)
                     /gene="fetA"
                     /locus_tag="OO471_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6242410.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13621..14538
                     /locus_tag="OO471_001058"
     CDS             13621..14538
                     /locus_tag="OO471_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6242411.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            14535..14993
                     /locus_tag="OO471_001059"
     CDS             14535..14993
                     /locus_tag="OO471_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6242412.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(14994..15401)
                     /gene="cueR"
                     /locus_tag="OO471_001060"
     CDS             complement(14994..15401)
                     /gene="cueR"
                     /locus_tag="OO471_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6242413.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(15526..16818)
                     /locus_tag="OO471_001061"
     CDS             complement(15526..16818)
                     /locus_tag="OO471_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6242414.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(16821..17753)
                     /gene="glsA"
                     /locus_tag="OO471_001062"
     CDS             complement(16821..17753)
                     /gene="glsA"
                     /locus_tag="OO471_001062"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6242415.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            18015..20519
                     /gene="copA"
                     /locus_tag="OO471_001063"
     CDS             18015..20519
                     /gene="copA"
                     /locus_tag="OO471_001063"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6242416.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(20734..21075)
                     /locus_tag="OO471_001064"
     CDS             complement(20734..21075)
                     /locus_tag="OO471_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6242417.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            21213..22007
                     /locus_tag="OO471_001065"
     CDS             21213..22007
                     /locus_tag="OO471_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6242418.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            22211..22690
                     /gene="ybaK"
                     /locus_tag="OO471_001066"
     CDS             22211..22690
                     /gene="ybaK"
                     /locus_tag="OO471_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6242419.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(22727..24379)
                     /gene="ushA"
                     /locus_tag="OO471_001067"
     CDS             complement(22727..24379)
                     /gene="ushA"
                     /locus_tag="OO471_001067"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6242420.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            24597..25817
                     /gene="fsr"
                     /locus_tag="OO471_001068"
     CDS             24597..25817
                     /gene="fsr"
                     /locus_tag="OO471_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6242421.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            26055..27731
                     /locus_tag="OO471_001069"
     CDS             26055..27731
                     /locus_tag="OO471_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6242422.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27864..29168)
                     /gene="gsk"
                     /locus_tag="OO471_001070"
     CDS             complement(27864..29168)
                     /gene="gsk"
                     /locus_tag="OO471_001070"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6242423.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            29320..30279
                     /gene="aes"
                     /locus_tag="OO471_001071"
                     /pseudo
     CDS             29320..30279
                     /gene="aes"
                     /locus_tag="OO471_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            complement(30276..31238)
                     /gene="hemH"
                     /locus_tag="OO471_001072"
     CDS             complement(30276..31238)
                     /gene="hemH"
                     /locus_tag="OO471_001072"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6242424.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(31370..32014)
                     /gene="adk"
                     /locus_tag="OO471_001073"
     CDS             complement(31370..32014)
                     /gene="adk"
                     /locus_tag="OO471_001073"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6242425.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            complement(32195..34069)
                     /gene="htpG"
                     /locus_tag="OO471_001074"
     CDS             complement(32195..34069)
                     /gene="htpG"
                     /locus_tag="OO471_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6242426.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(34179..34784)
                     /gene="recR"
                     /locus_tag="OO471_001075"
     CDS             complement(34179..34784)
                     /gene="recR"
                     /locus_tag="OO471_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6242427.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(34784..35113)
                     /locus_tag="OO471_001076"
     CDS             complement(34784..35113)
                     /locus_tag="OO471_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6242428.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(35166..37091)
                     /gene="dnaX"
                     /locus_tag="OO471_001077"
     CDS             complement(35166..37091)
                     /gene="dnaX"
                     /locus_tag="OO471_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6242429.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    complement(35769..35833)
                     /gene="dnaX"
                     /locus_tag="OO471_001077"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(37220..37771)
                     /gene="apt"
                     /locus_tag="OO471_001078"
     CDS             complement(37220..37771)
                     /gene="apt"
                     /locus_tag="OO471_001078"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6242430.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(37924..38301)
                     /locus_tag="OO471_001079"
     CDS             complement(37924..38301)
                     /locus_tag="OO471_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6242431.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            38371..38898
                     /gene="priC"
                     /locus_tag="OO471_001080"
     CDS             38371..38898
                     /gene="priC"
                     /locus_tag="OO471_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6242432.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38912..39073
                     /gene="rsmS"
                     /locus_tag="OO471_001081"
     CDS             38912..39073
                     /gene="rsmS"
                     /locus_tag="OO471_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6242433.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(39285..42647)
                     /gene="mscK"
                     /locus_tag="OO471_001082"
     CDS             complement(39285..42647)
                     /gene="mscK"
                     /locus_tag="OO471_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6242434.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(42775..43422)
                     /gene="acrR"
                     /locus_tag="OO471_001083"
     CDS             complement(42775..43422)
                     /gene="acrR"
                     /locus_tag="OO471_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6242435.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            43564..44757
                     /gene="acrA"
                     /locus_tag="OO471_001084"
     CDS             43564..44757
                     /gene="acrA"
                     /locus_tag="OO471_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6242436.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            44780..47929
                     /locus_tag="OO471_001085"
     CDS             44780..47929
                     /locus_tag="OO471_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6242437.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            48475..48849
                     /gene="tomB"
                     /locus_tag="OO471_001086"
     CDS             48475..48849
                     /gene="tomB"
                     /locus_tag="OO471_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6242438.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            48875..49093
                     /gene="hha"
                     /locus_tag="OO471_001087"
     CDS             48875..49093
                     /gene="hha"
                     /locus_tag="OO471_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6242439.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            49265..49816
                     /gene="maa"
                     /locus_tag="OO471_001088"
                     /pseudo
     CDS             49265..49816
                     /gene="maa"
                     /locus_tag="OO471_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            49932..50402
                     /locus_tag="OO471_001089"
     CDS             49932..50402
                     /locus_tag="OO471_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6242440.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            50566..52116
                     /gene="pdeB"
                     /locus_tag="OO471_001090"
     CDS             50566..52116
                     /gene="pdeB"
                     /locus_tag="OO471_001090"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6242441.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(52158..52511)
                     /locus_tag="OO471_001091"
     CDS             complement(52158..52511)
                     /locus_tag="OO471_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6242442.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(52631..52727)
                     /gene="ffs"
                     /locus_tag="OO471_001092"
     ncRNA           complement(52631..52727)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO471_001092"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            52890..53201
                     /locus_tag="OO471_001093"
     CDS             52890..53201
                     /locus_tag="OO471_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6242443.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT        12675 a        14016 c        13688 g        12832 t
ORIGIN      
        1 aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc
       61 gcgctcgccc gccgcccagt cacctgccgg taaattgtgc agcgtatgtt cgagtttatt
      121 actcgccagt acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac
      181 gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa
      241 taacatcgat aaattagcag agaaacgtaa catcatttat ctcccttatt catagtgcat
      301 gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac
      361 gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa cttccacgac
      421 taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc
      481 ttccggtttg aagacccgaa tagctttaca acctaaacct tccgctactt ttacgtggtc
      541 aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt gcacgcagta
      601 gtccatgtca aaagcgcgtt gtgactgacg aatcaggccg agataggcgt tattcaccag
      661 cacatggatg tacggaatgt tgaactgcgc gccaacggct aactcttcaa tcaggaactg
      721 gaagtcaaag tcgccagaaa tcgccaccac attgcgtttc ggatcagcgg cacaaccccc
      781 cagcgccgcc ggaatcgtcc agcctaacgg accagcctga ccacagttga tccagtggcg
      841 gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacagaccaa tagtggtgac
      901 ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct gcggtttcac
      961 cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttacgctgct ggcagtcggc
     1021 gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg ccacttcaac
     1081 cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac acagcacgcg
     1141 accaatttgc gtcggctcaa tatcaatatg aacgattttg cgcccttcgg tgtatttctc
     1201 taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc
     1261 cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga ccgaccatcc cggccatcag
     1321 ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt
     1381 cagttcagca aactgttgta acagtgcagc tgcgtcagca ttgattaccc cgcccccggc
     1441 aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga tctgcatacg
     1501 gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa actcgatttc
     1561 cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac cagaacgcat
     1621 caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa ctgtaaccgc
     1681 cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg
     1741 cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc
     1801 ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc
     1861 gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaaacac cttccacatg
     1921 acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattaat
     1981 tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca catacattgc
     2041 cgcgtcaacg gctctcattt ttgccatttt attcctacct ctattagttg gaaaaatttt
     2101 ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacactat
     2161 ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca
     2221 ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg
     2281 gcaacaactc taccgacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca
     2341 tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcaag
     2401 gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg
     2461 ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc ggaagcatgc
     2521 agcggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca ctctaactga
     2581 ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt
     2641 aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac
     2701 gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg actgtcctga
     2761 tagacaaaat ccgctgcttg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa
     2821 tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc
     2881 agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg ctccgcctgt
     2941 cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga
     3001 gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac
     3061 agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg
     3121 tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt
     3181 aattcacccc ctgttcaccg ttggtgataa acgcccgcag cgttgacagg tctggtttgt
     3241 cgtcgcctaa cgccacgacc accacaaaca cttcaccttt cattgggata aaggcctgag
     3301 tgcccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc ggttgcgcgc
     3361 ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt
     3421 aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt tcgattacgt
     3481 cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt
     3541 ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc aataacaggc
     3601 aatctaaaac cttaagagaa caacattaat acaaaagttg gaatgttttt ctaaaactag
     3661 atttagtcca caaattgaac aggattatca tgtaaaaata ctgataaatc agaggataag
     3721 aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat ccagaaacct
     3781 tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca gaacgattat
     3841 gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat accggagtag
     3901 cgctgttttt ccgtacgact cgtagcgtaa cgttgacagc ggctggcgag catctacttt
     3961 cccaggccag agactggctg agctggctgg aaagtatgcc cagcgagctg caacaggtga
     4021 atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctat aacccccagg
     4081 ccgtcgccca gttgctggcg tggctgaatg agcgttaccc ctttacccag tttcacatct
     4141 cccgacaaat ctatatgggc gtctgggact cgctattgta cgaaggtttt tcgctggcta
     4201 tcggggtcac gggaactgag gcgctggcaa atacctttag tcttgatccc ttaggatcgg
     4261 tgcaatggcg atttgtcatg gcggcggatc atccgctggc gaacgttgaa gagccgctaa
     4321 cagaagcgca gttgcgtcgc tttccggcgg tcaatattga agacagcgcc ggcaccttaa
     4381 ccaaacgcgt cgcctggcga ttgccggggc aaaaagagat tattgttccc gatatggaaa
     4441 cgaaaatcgc cgcccatctg gcgggtgttg gcattggttt tttgccaaaa tcactttgcc
     4501 agtcaatgat cgataatcaa caactggtca gccgggtaat cccaacgatg cgccctcctt
     4561 cgccattgag tctggcatgg cgcaaatttg gcagctgcaa agcggtagaa gatattgtga
     4621 ccttgtttac ccaacacagg ccggaaatca gcggattttt agaaattttc ggcaccccac
     4681 gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca agctgatttg
     4741 aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg ccctgctgat
     4801 tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag gcgcaatgcc
     4861 cgccgctatc aatctgccgt taatggataa cgatgaacgc gcctccgttg gcacctgcta
     4921 taaacagcaa ggctcagacg cagcgctggc gctggggcat aaactggtgg cgggtgaaat
     4981 tcgtcagcag cgtatgtacg cctggcgggc agcgtgcctg caaaatccgc aaggtattct
     5041 ctgctgcgcc cgtggcggtc aacgttcaca tattgtgcaa cgctggctgc atgaagcggg
     5101 gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg cgattcaggc
     5161 gaccattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtaccg gcagcggtaa
     5221 aacgctatta gtgcaacaac agccaaacgg tgttgatctg gaagggctgg cgcgtcatcg
     5281 cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt ttgaaaacct
     5341 gctggctgcc gaaatgctaa aaaccgacgc ccgtcaggat ttgcgcctgt gggtgctgga
     5401 agatgaaagc cggatgatcg gttcgaatca cctgccggaa tgtctgcgcg agcgaatgac
     5461 tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgaac gcctgaatga
     5521 agagtatttc ttgcgtatgc atcatgattt tacccacgct tatggcgacg aacagggctg
     5581 gcaggaatat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc ggctggggtt
     5641 acagcgctat aacgaactcg ctgcccagct ggatacggcg ctgacaacgc aactcaccac
     5701 cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttaaagaat attacgaccc
     5761 aatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg gtgagtgggc
     5821 ggaagtggcg gtatgggtta aggcgcagtg atgggcggta ttctgaaacg gcgattgtcg
     5881 tgagaacaaa atggttgcac cagaatacag atataaaaaa accgacatga cagtcggttt
     5941 ttttattttt tccgcccaag acttggtggt tccgggcaga aattaaacag tcctcatcta
     6001 gcaagtgaat tataagtcag gatgcctaca tattcaaaca gctatgttgg cgatacaatc
     6061 ccgacgctag tagtgatact gcctcaaggg attaccgcag tgcggaaaag tacttttaag
     6121 tcctggtcag gatacaggtg gtgctgattc tggtgctcta atttggagag agacagtcct
     6181 catctagcac atgaagaaaa tcatagttat aggcaacgta agggtaagct gtgattgtct
     6241 ttctccctta agggagcaac ggatgaaaac ttttctcgat aaagttctca tcgttcttaa
     6301 ggcgctaatc gcgctgctgg aactgatccg tcagtttatt gaataactga ggaaacgtag
     6361 ctaaggccga aggtttacct ccttcggcct ttaaaaaaac cgacatgaca gttggtttca
     6421 tatttattgc caaaatgcac actgttcatg ccagatgcgg cgtaaacgcc ttatccggcc
     6481 tacacaatct tgcaaaatca atatattgca cgctcttcgt aggcctgata agcgtagcgc
     6541 atcaggcaat ctaacacctg tcatcacccc acaaactgcc tgaacagcgc cttaccctta
     6601 accagtcgcg cacccagcca gccgccgaaa agcgacagca gcagcgcacc gctgcacggc
     6661 agaacaatcc acaatcgcca gtctggctcc cacgggaaat caaacacttt cgcctgcaac
     6721 actgccagtg ccgtttccgc accaattgcg gccaccaggc cggaaacaaa cccaagcatg
     6781 gcgaacatca caccacaatg tcgtgcgcag cagttttttc cccgcaccga gtgtgcgcca
     6841 taccaccagc tcctgatgac gctggcgcat tcccacctgc acctgtgcca gcaacagcaa
     6901 cataccgcag gcggtgacca gcaccaccat cacctccagc gcccgactta cctgctccag
     6961 cacctgaccg acctgtttta aaatcgcgcc aatgtctaac aggctaatgg tcgggaactg
     7021 gcggttgagt tgcgtcaaca tgccgttgcc attctcccag cggaaactgg taagccagct
     7081 ctgcggttgc ccgtctaatg ccccttcagg gaaaataaaa tagaaattag gccgcagact
     7141 ttcccagtcc actttgcgca ggctggtcac tttagcgcgg aactcctggg tatcgcccat
     7201 aaaagtcacg gtatcaccga gggcaacgtt taagcgtttc gccagcccct cttccatcga
     7261 cacttcatcg gcttttggcg gccagttacc ggcgacaatc ggattatgat cgggccgcgt
     7321 attttgccag gtaagattga gttcgcgatt aagcgcctca tcttcattac cttctgtcgg
     7381 cttatcatta atcgccgtca gccgcgcccg cactaccgga taaaatgatt ccgggactat
     7441 atgatgttcc gcgaggaacg cttttagcgg tgctacctgt tctgtggcga tgttaattaa
     7501 aaagtagttc gggctttctg gcggtagctg ctgctgccag cggtcgagca gatcgccacg
     7561 cagcaccagc agcagtgcca gcagcataaa ggagagcgaa aatgccgaaa gctggcttaa
     7621 cgttgaccac ggctgacgta acaggcggct aaccgccagg cgcagaggca gcgatttcag
     7681 cgtcatgcgg cgaagtacat tcagcagcat ccagcccagc acaccgcaca gcaaagccag
     7741 tactaccgcg cccgccagca ccgcccaaag cagcatgctg ccacccatta atccggcgag
     7801 cagcagcaca accaccacac tgacaatcgg cagataaaac ttcagcggcc agacgttcgc
     7861 gaccacatca ttacgtaata cgcgtaaagg ctgcgttgcc agcaacaagc ggtatggtcg
     7921 tagccccacc agcagcgaga tgaccgtcat ggtgccaagc gcccacagcc acggccagag
     7981 gctggctggc ggtagtgcag caggcagaac gggcttgagc agcaccatca acacgttttc
     8041 gaacaacagg cctatggccc caccggtaac ggctgaaagc gtcagcacca tcaaccactg
     8101 accgacgatt agcttacgca gttgcgctcg ccctgccccc agcgttttga ggatcgccac
     8161 cagatcgtag cgactgcgac agtaatgatt catcgctacc gccaccgctg ccactgccag
     8221 cagcaaggtc agaagcgccg aaagcagcag gaactgttgc gagcgttcca tcgatcgccc
     8281 cagcgcgcct tcgtcctgtt ccagaccgta ccagcgttgt tcgggtttaa gctgaggtaa
     8341 caaccatttc tcatagccgt cgagctggtt ctcgttgccg ccgaatttat aacgccaggt
     8401 gacccgactc cccggctgca cggctccggt tttatcgaca tccgccagat tcatcatcag
     8461 acgcggagcc atctggaagg ggttaaaacc ggaatccggt tcctgaatca cttctccggc
     8521 aatccgcaag gtggcatcgc ccacgtcaat ggtgtcgccc gttttcaggt taagcagtgc
     8581 catcaggcgt ggggccagca atacgctgcc cgcctgcggt ttcaggccag ggggattagt
     8641 ttgcagaccg ccatacatcg ggtagatatc atccaccgct ttgacgttcg ccagctgcgg
     8701 tgtgtcgcct gcaaaggtca ttgtggcgaa agtcagctgc ttgccgactt tcaggccgcg
     8761 cttttgcgct tcctccagcc acgcttgcgg cacttcgcgt gaactgcgca acgcccgatc
     8821 gcccgccata aactcacggc tttgctggct taagcccttc tccatgcgat cgctgatatt
     8881 gcccagcgcc agcacgcagg ccaccgccag gcttagcgcc agccagacaa ttaatagcga
     8941 cggcgaacgc cattcgcgcc agaaccaacg tgcaatcatg cttcctcctg caactgcccg
     9001 ttcaccagcc gtaagcagcg gtcgcagcgt gccgccagtt gcaggtcgtg ggtcaccata
     9061 atcaacgtgg tgccatgttc acggttgagg gaaaacagca ggtcggcaat tttatcgccc
     9121 gtctggcggt caaggttgcc ggtgggttcg tcggcaaaca gcacatcagg tcgaccatta
     9181 aaggctcgtg ccagcgccac tcgttgctgt tcaccgccgg aaagctgtgc cggaagatga
     9241 tccagacgtt tacccagccc taactgttcg agcaacgctt tcgccccgtt acgactttcc
     9301 gcgctactct caccgcgcag cagagccgga agctcgacgt tttccagcgc gttaagggta
     9361 ggaattaaca taaatgactg aaaaacaaag ccgacgtgct tcgcgcgcaa ctttgcccgc
     9421 gcttcttcgt ccatattatg tagcggttgt cccaccagac tcacttcgcc actgctgccg
     9481 tcatcaagcc cggcgaggat cgccagcaag gttgacttac ccgatcccga ctcgcccacc
     9541 agtgcgatgg tctcgccacg tttgacaacc agctcaactc cggtgaggat ggagagttca
     9601 tgctccccct gaccgacgga cttcttaaga tgatgaactt caacaatgtt ttccgctggc
     9661 atttgccctt cctgttcctg gtcctgttaa ccttccgtgc cgccgcagcg gacacgttat
     9721 tgattctggg tgatagcctg agcgccgggt atcgaatgtc tgccagcgcg gcctggcctg
     9781 ccttgttgaa tgataagtgg cagagtaaaa cgtcggtagt taatgccagc atcagcggcg
     9841 acacctcgca acaagggctg gcgcgccttc cggctctgct caaacagcat cagccgcgtt
     9901 gggtgctggt tgaactgggc gcaatgatgg tttgcgtggt tttcagccac agcaaaccga
     9961 gcaaacgctg cgccagattt tgcaggatgt caaagccgcc aacgctgaac cattgttaat
    10021 gcaaatacgt ctgcctgcaa actatggtcg ccgttataat gaagccttta gcgccattta
    10081 ccccaaactc gccaaagagt ttgatgttcc gctgctgccc ttttttatgg aagaggtcta
    10141 cctcaagcca caatggatgc aggatgacgg tattcatccc aaccgcgacg cccagccgtt
    10201 tattgccgac tggatggcga agcagttgca gcctttagta aatcatgact cataaagcaa
    10261 cggagatcct gacaggtaaa gttatgcaaa aatcggtctt aattaccgga tgttccagtg
    10321 gaattggcct ggaaagcgcg ctcgaattaa aacgccaggg ttttcatgtg ctggcaggtt
    10381 gccgaaaacc gaatgatgtt gagcgtatga acaacatggg atttaccggc gtgttgatcg
    10441 atctggattc accagaaagt gttaatcgcg cagcagacga ggtgatcgcc ctgaccgata
    10501 attgtctgta tgggatcttt aacaatgccg gattcggcat gtatggcccc ctttccacca
    10561 tcagccgtgc gcagatggaa cagcagtttt ccgccaactt tttcggcgca caccagctca
    10621 ccatgcgcct gttacccgcg atgttaccgc acggtgaagg gcgtattgtg atgacatcat
    10681 cggtgatggg attaatctcc acgccgggtc gtggcgctta cgcggccagt aaatatgcgc
    10741 tggaggcgtg gtcagacgcg ctgcgcatgg agctacgcca cagcggaatt aaagtcagcc
    10801 tgatcgaacc cggtcccatt cgtactcgct tcaccgacaa cgtcaaccag acgcaaagtg
    10861 ataaaccagt cgaaaatccc ggcatcgccg cccgctttac gttgggaccg gaagcggtgg
    10921 tcgacaaagt gcgccatgct tttattagcg agaagccgaa gatgcgctat ccggtaacgc
    10981 tggtgacctg ggcagtaatg gtgctcgagc gcctgctgcc tgggcgcgtg atggacaaaa
    11041 tattgcaggg gtgagttgaa gcgcgcgctt aagcccccat gtcaaagaaa atatcgacaa
    11101 cagagagcga ctccatgtcc gtagaaaata ttgtcaacat taacgaatct aacctgcaac
    11161 aggttcttga acagtcgatg accactccgg tgctgttcta tttttggtct gaacgtagcc
    11221 agcactgttt gcagttaacc ccgatcctgg aaagcctcgc ggcgcagtac aacgggcaat
    11281 ttattctggc gaagctggac tgcgacgcgg agcagatgat tgccgcccag tttggtctgc
    11341 gcgcgattcc gaccgtgtat ctgttccaga acgggcaacc ggtagatggc ttccaggggc
    11401 cgcaaccgga agaggcgatc cgcgccctgc tggataaagt gctgccgcgc gaagaagagc
    11461 tgaaagcgca gcaggcgatg caactgatgc aggaaggcaa ttacaccgat gccctgccat
    11521 tgctgaaaga cgcctggcag ttgtcgaatc agaacgggga gatcggcctg ctgctggcag
    11581 aaacgctgat tgcgctgaac cgttctgaag atgcggaagc ggtgctgaaa accattccgt
    11641 tgcaggatca ggacacccgc taccaggggc tggtagcgca aatcgaactg ctgaagcagg
    11701 cggctgataa gccggaaatt caacagttgc aacagcaggt ggcggagaat ccagaagatg
    11761 ccgcactggc gacgcaactg gcgctgcaac tgcatcaggt tgggcgcaat gaagaggcgc
    11821 tggagttgct gttcgggcat ctgcgtaaag atctcaccgc cgcagacggt cagacgcgta
    11881 aaacgttcca ggagatcctc gctgcgctgg gtacgggtga tgcactggcg tcgaagtatc
    11941 gccgccagct gtatgcattg ttgtattgat attccgttgt tcggcacatg aaccacaacg
    12001 ccggatgcga tgctgcgcat ccggcatcgc atcatttctt cttcaattga gtcaccacca
    12061 actggtggcg cgaattataa aacttacgat aggttaaata gcaggcaata atggtcgaca
    12121 agctggcggt tgagagcagc ataaaggtca ccataatctg atatttaatc gccttcaccg
    12181 gatcaatccc ggcaaatatc agcccggaca tcattcctgg taaactcact aagccaaccg
    12241 tttttgctga atcgaccgtc ggaattaaag ccgcgcgaat actgtcgcga atcaatatcg
    12301 ctgaagcctg cttcggcgtc gcgccaagac tcagtttctc ctgaatctgt tgctgttcgc
    12361 taatgacccg ttgccctaaa ttgttgtaac acaaccccac cgctaccatg gcgttaccgg
    12421 caatcatccc ggcgacaggg atcacctgca tcgggataaa ttcaatcgcc cctgagagaa
    12481 tcagcaccgc cagggtaatt cccgccccga ccgtaatggc aataaacgat gagataaaag
    12541 ctttagcaat atatttactg cgtttttgcg cgttccacgc cgcattaaag cagataaata
    12601 acaccatcaa taatgtcagg ctggcatcat ccacgctgaa aatatacttc agcacatagc
    12661 cgacaataat cagctgaatt atcgctcgcc cgacgctcca tagaatatct ttctccagcg
    12721 ccagtttttc tttatggcta attaagattg ccaccaccac cagcattaat gccagtgcta
    12781 atgattcgtt agtaatatta tgcgagttca tagcgtgctt cctgcatttc tccggcatgc
    12841 ggttgcagtg taatcacttt atccgcatga ttaatttcgt ctttatcgtg tgtcacccac
    12901 agcacggcaa tattttgctc gcgcacataa cgatggatca tctcattgac gttatgttta
    12961 ttactttcat ccagcgcact ggttatttca tccagcaata aaaccttcgg cataaattgc
    13021 aggttacgaa tcaatgagat gcgttgtttt tcaccaccag atagctcggc gatattcttc
    13081 gtcaaaatgc tgtccggcaa agcgaagcgt tcgagaaaat cgagaaaaat ggccgggtca
    13141 ggctgctggt cacggatctg ccagggaaag atcagattat cgtataccgt atcgccaaac
    13201 agcgtcggcg tctgggcgca gtaagagact tgctggcggt agatttctgg ttttagtgtg
    13261 ctgacatcct caccttcaaa cagtaacgtt ccgctggttg ggctgatcaa tgaagcaact
    13321 atttttagca gcgtactttt gccacaacca gaaggaccgg taattaactt aaattcgcca
    13381 gcacgcagcg aaaaattgat gttattaaga atcttcgtat cacccgccag atatcctacg
    13441 ttttgtagct gaagcaaagg actattttcc tgcatcgctg ttcccttttt ctgattttta
    13501 ctaaaaacag tttatccttc gcaggaataa gggggaactc tctttcagta atcaggtata
    13561 atttgtttaa tcaagcagca attttgctga ttatggacgg tacaacagga ggtttttccg
    13621 atgcttatct ttatcccgat tctcattttt gtcgcgctgg tcattgtcgg cgcgggcgtc
    13681 aaaatcgtgc cgcagggcta tcaatggacg gtagaacgtt ttggtcgcta taccaaaacg
    13741 ttacagccgg ggctcagtct ggtggtgccg tttatggatc gcattggtcg caagatcaat
    13801 atgatggagc aagtgctcga tatcccttcc caggaagtta tctcgaaaga taacgccaac
    13861 gttaccatcg acgcagtctg ttttattcag gtgattgacg cgccacgcgc tgcctatgaa
    13921 gtcagcaatc tggagctggc gatcatcaac ctgaccatga ctaacatccg taccgtgttg
    13981 ggttcaatgg aacttgacga aatgctctct cagcgcgaca gcatcaactc acgcctgctg
    14041 cgtattgtcg atgaagccac caacccgtgg gggattaaag tcacccgtat tgaaattcgc
    14101 gacgtgcgcc caccggcaga gcttatctct tcaatgaacg cgcagatgaa agcggaacgt
    14161 accaaacgcg cttacattct tgaagcggaa gggatccgtc aggcggaaat cctcaaagcc
    14221 gaaggtgaaa aacagtcgca aatcctgaaa gcggaaggcg aacgtcagtc ggcgttttta
    14281 caggctgaag cgcgtgaacg ttccgccgaa gcagaagccc gcgccaccaa aatggtgtct
    14341 gaagctatcg cctccggtga tattcaggcg gtgaactact tcgtagcgca aaaatacacc
    14401 gaagcgttac agcagatcgg ttcctccagt aacagcaaag tagtgatgat gccattagag
    14461 gccagcagcc tgatggggtc gattgccggg attgccgagc tggtgaaaga cagcgccaac
    14521 aaacggactc agccatgatg gagttaatgg tcgttcatcc acatattttc tggctcagtc
    14581 tcggcgggtt gctgctggca gccgagatgc tgggcggaaa tggttatttg ttgtggagcg
    14641 gcgtggcagc ggtgattacc ggcctggtgg tctggctggt gccgctgggt tgggagtggc
    14701 aaggggtaat gtttgccatc ctgacgctgc tcgccgcctg gctgtggtgg aaatggttgt
    14761 cgcggcgggt gcgcgaacaa aagcacagcg atagtcattt aaaccagcgc gggcagcagc
    14821 tgattggccg acgttttgtg ctggaatctc cgctggtcaa cgggcgcggt catatgcgcg
    14881 tcggtgacag ttcatggcct gtcagcgcca gcgaggatct cggtgcaggt acgcatgttg
    14941 aagtcattgc gatagaaggg ataaccctac atatccgagc cgtctcgtct taatcaccct
    15001 gcccgatgat gacagcagcc ggagagattt tcgataatcg ggcagtcggc gctgtcatcg
    15061 ccagggcagg cattcgccag tgccagcagc tggtcgcgca tggattgcag ctcctcaatg
    15121 tgtcgttcga tctccgccac cttctccagc gtgcgccgtt tgacgtcggc gctgtgccgc
    15181 tgcgggtcgt taaacagatt caccagctcg ccgctctctt ccaggttaaa gcccacctgc
    15241 cgtgcctggc gcagtaaggt cagttcgttg agatgctgct gcgtgtaggt gcgataaccg
    15301 ttttcgctgc gcatcggcgg cgtcaccagc cccttctctt catagaagcg aatggctttg
    15361 ctggtcaggc cggtaatttt tgctacatcg ctgatgttca tcgttcgcgc aacgcctcct
    15421 gtgctttgtt aaagggtttc aacagatatt caagcacgtt tttttgccag tgcgaatatc
    15481 gaccgccacc acaataatac aggaagtact gcgcacagag aggtgttata cggttttatt
    15541 acgcttcatg accattgcca caataaggct gagtaagatt gcaccaataa tccatatcaa
    15601 cgcacggctg ccctgggacc acaggctgta gataaagcca acgatcacca acaacatcac
    15661 caacgtacca acgataagaa tcggcaacga ggcgtgaata tcatgacgca ggcggatcgc
    15721 caccacaaac accgccagat agcagatcaa aaaggtggcg ctggcaacgc tggcgagtga
    15781 gtctaaattt agcgccgccg tcatcagcat tatcaacacc acgacgatga tattgcccca
    15841 ggtactctgc cgccacaggg atttattcat tagcttcggc agttcgcgtt cgctgcccat
    15901 gttgtccatg atgttaaaca cggcgaacag gttcgcgtta atgcctgaag ccgtcgccag
    15961 taaagcgccg atgacgacga tcacataacc cacatgcccg agcagcggag aagcagcctg
    16021 cgctacggcg gtatcagcat atttttctaa ctctaatgcc gatacatcgc taagcaaaac
    16081 cagcgccagc gagatataaa gcaacgtggt aacgccaatc gccaccagaa acgcccgtgg
    16141 cataatgacc tgcggatctt tcactttatc cgccgcgttc gccatcatgc caaagcccgc
    16201 ataggcaagg aaggttatcc caatacagga gaagaacgca ccggagctgg ggggcgcaga
    16261 gacggaaata tgcgccggtt gcagcgacca gacaccggca ataatcagca ataacaggat
    16321 catcatttta atgccgacga ggatcacttc cagccgccct accgcatggt tgcttaagga
    16381 gttgaaaagc gtcatcaccg caatgatccc caacgcgtag agcaaaataa ggtgctcctc
    16441 ctggctgcct tcatgcaaaa actgcacggc ataagcgcca aaagcacggg caaccatggc
    16501 gatgctcacc gccagcgtca acaggtatag caacgagagc gctagcgaaa agacgccgtt
    16561 gcctaatccg cgacgaaaaa agtcgataat accgccattg ctgggatagc tcgcccccag
    16621 acgcgcatag gcataaccgg aaaacatagc cacaataccg ccaaaagcaa aggcgcccca
    16681 ggtcgaggct tccattagca acgcagcctg ccccagcagc gcgaagatcc ccgcccccac
    16741 cattgcccca ataccgatgg aaacgacgtt ccatagaccg aggggtttgt taccgttatt
    16801 accttccgtg ttcatcatga tcagccctta aacacgttat agccgagttg cttagcgacc
    16861 gatgccacca ttttttgacc gcgaacacta ttgccttctt cgtccagcgg tggtgagaac
    16921 gcggcaattc ccatcacgcc agggacgacc gccagaatac cgccacctac accgcttttg
    16981 cccggtaaac caacgcgata cgcccagtca ccggagcgac catacagccc ttccatcatc
    17041 atttcggcca gaatgtacgg cacgttgtcg gcctgaagaa cgcgtttatg cgtcaacgga
    17101 ttcacaccac ccgccgccag cgtcgcgcca agcgttgcca gttcaacagt attgatgagc
    17161 gtagagcact gacgggtgta cacgtcacag gcttccattg catcacaata gagatatccg
    17221 gcggagtaca gcagccaggc aatggcccga ttatggaagt tggttgtttg ttccgactgg
    17281 ttgacttcgt cagagagcgc tacctgctca ccagccagtt gctgttggat atgtaaaatt
    17341 cgctgccagc gttgttcggc gttttcagcg ttaatcaggc tggtggtggc aatagcgcca
    17401 gcatttacca gtggcgaaag cggtttgccg ccatgcaact ctaaggcgat aactgagtta
    17461 aagggcaatc cggtcgggtc agcgccaatt ttgtcctgta ccgcctgcgg gccgacatct
    17521 tctaacgcaa gggctaacgt acagacttta gagatggatt ccagtgcaaa gcggtaatca
    17581 ctgtcacccg cactatagac gttgccatcg caggtcacga tagccactgc cgccagttga
    17641 cctggtacat tcgccagaaa gggaatgtaa tcggcatttt gtccgccgtt aagtgagtga
    17701 aattgggtgt aagcctgatc cactgcctgc tgtaatttgt ttgcatctaa catcttttgt
    17761 taactccttt ttatagatgc gggaggtaat tcctcacccc agtgccgatt ttcaggcatc
    17821 ctgatttaac ttagcacccg caacttaact acaggaaaac aaagaaataa atgtctaatc
    17881 ctgatgcaaa tcgagccgat tttttaatct ttacggactt ttacccgcct ggtttattaa
    17941 tttcttgacc ttccccttgc tggaaggttt aacctttatc acagccagtc aaaactgtct
    18001 taaaggagtg ttttatgtca caaactatcg acctgaacct ggacggcctg tcctgcggtc
    18061 actgcgttaa acgcgtgaaa gaaagtcttg aacagcgccc ggatgtggaa caagcggatg
    18121 tgtctatcac tgaagcgcac gttaccggga ctgccagtgc agaacagctg atcgaaacca
    18181 tcaaacaagc gggttatgac gcatctgtaa gccacccaaa ggctaaaccg ctggcggagt
    18241 catcaatccc gtcggaagca ctgacagcgg tttctgaggc gcttccggca gcgaccgccg
    18301 atgacgatga cagccagcag ttgctgctga gcggcatgag ctgcgccagc tgtgtcaccc
    18361 gcgtacaaaa tgcgctgcaa agcgtaccgg gcgtcactca ggcacgggta aacctggcgg
    18421 agcgtactgc gctggtgatg ggcagtgcct ccccacaaga tttagtgcag gcggtggaaa
    18481 aagcgggcta cggcgcggaa gcgattgaag atgacgcgaa acgccgcgaa cgccagcaag
    18541 aaaccgccgt cgctacgatg aagcgcttcc gctggcaggc aattgtcgca ctggcggtgg
    18601 gtatcccggt gatggtctgg gggatgatcg gcgataacat gatagtcacc gccgacaacc
    18661 gcagcctgtg gttggttatc ggcctgataa ccctggcggt gatggttttc gccagcggcc
    18721 atttttaccg cagtgcatgg aaaagcctgc tgaacggtgc agcgacgatg gatacgctgg
    18781 tggcgctggg aacgggcgtg gcgtggctct attcgatgag cgtcaacctg tggccgcagt
    18841 ggttcccgat ggaagcgcga catctttact atgaagccag tgcgatgatt atcggtctga
    18901 tcaatctcgg ccatatgctg gaagcacgcg cacgtcagcg ttcttctaag gcactggaga
    18961 aattactcga tttaacccca ccgacggcgc gcctggttac tgacgaaggt gaaaaaagcg
    19021 tgcctctggc agaagtgcag ccaggtatgt tgctgcgcct gacgaccggc gatcgcgtgc
    19081 cggtagatgg cgagattacc cagggcgaag catggctgga tgaagcgatg ctgacgggcg
    19141 aaccaatccc gcagcaaaaa ggcgaaggcg atagcgtcca tgccgggaca gtggtacagg
    19201 acggcagtgt gctgtttcgt gccagtgcgg ttggcagcca taccacgcta tcaaggatca
    19261 ttcgcatggt gcgccaggcc cagagcagca agccagaaat cggtcagctg gcggataaaa
    19321 tctcagccat atttgtgccg gtagtagtgg ttattgcgct tgtcagtgcg gcaatctggt
    19381 atttctttgg tccggcaccg cagattgtct ataccctggt gattgccact acggtgctga
    19441 ttatcgcctg cccgtgtgcg ctgggactgg cgacgccgat gtcgattatt tccggcgtcg
    19501 ggcgggcggc tgagtttggc gtgctggtgc gggacgccga cgcgctgcaa cgcgccagta
    19561 cactcgatac cgtcgtgttc gataaaaccg ggacgctgac cgaagggaag ccgcaggttg
    19621 tcgcagtgaa aacatttgct gatgttgacg aagcgcaggc gctgcgtctg gcggcggcgc
    19681 tggagcaagg ttccagccat ccattggcac gagcgatcct tgataaagca ggcgatatgc
    19741 agctaccgca ggtcaacggt ttccgcacat tgcgcgggct gggcgtgagc ggtgaagcag
    19801 aaggtcatgc gttattgctg ggtaatcagg cgttgttaaa tgatcagcaa gtcgatacta
    19861 aagcgattga agccgatatt tcagctcagg catcgcaagg agcaacgcct gtgctgctgg
    19921 cgattgacgg gaaagcagta gccctgctgg ctgtacgcga tccgttgcgt agtgatagcg
    19981 tggcggcgct gcaacgcctg cataaagcgg gatatcgtct ggtgatgttg accggggata
    20041 acccaaccac cgccaatgcg atcgccaaag aagctggaat tgatgaagtg attgccggag
    20101 tgctgccgga cggtaaagcc gaagcgatca aacgtctgca aagtgaagga cgtcaggtgg
    20161 caatggtggg cgacggcatt aacgacgcgc cagcgctggc ccaggcggat gtcggcattg
    20221 cgatgggtgg cggcagtgat gttgccattg aaaccgcggc gattaccctg atgcgccata
    20281 gcctgatggg cgttgcggat gcgctcgcga tttcccgcgc aacgctgcgc aacatgaagc
    20341 agaacctgct cggtgccttt atctacaaca gcatcggtat tccggtcgcc gccggtattt
    20401 tgtggccgtt tactggaaca ctgcttaacc cggtagttgc cggagcggca atggcgctct
    20461 cgtcaattac cgtagtaagt aacgccaacc ggttgctgcg gtttaaaccg aaggaataag
    20521 tacgtcattg cggatgcgct gctttagggc ggcgcatttg gcatcgacgc atgactgccg
    20581 gatgcggcgt aaacgcctta tccggcctac ggttcaggca caggtttgta ggcctgataa
    20641 gacgcgtaag cgtcgcatca ggcaatggtg ctcggatgcc ggatgcggcg tgaacgcctt
    20701 atccggccta cgagatgaag cgatgttttg gccttacgcg accttttttg cacgctcttc
    20761 acggcgtgcc aaatattcag ccactgtttc aatccgtccc aattcttcct gggtgcgcat
    20821 gttgttttca acttcccaaa gatcaactgc cgcctggagg tttagcctaa aatcgactgt
    20881 ggtatcaaaa actttcgcca gacggaatgc catctcagtg gtgagtttac gattgttatt
    20941 gatcagtgca ctgacgcgat tacgatgaac atgcagcaac tctgctaact cattgatttt
    21001 caaatcgagc ggttccagat attcatagag aagaatatct ccgggtgtcg tcggttttct
    21061 tgttgcctgt ttcatggttt ttaactgttg cttccctgtg aatagcgatg caagaacata
    21121 ctgaatcata caaacacatt aaacaagtcc ccaactccat ttgcgccatc cctcaccgcg
    21181 cgctagcatg atatttcaca aagggaggtc gtatggatct gttgtaccgg gtaaaaacgc
    21241 tttgggccgc gctgcgcggt aatcattaca cctggcctgc catcgatatc accctccccg
    21301 gcaatcgcca ttttcatctg attggcagta ttcatatggg tagccacgat atggctcccc
    21361 tgcccacccg tttgctcaaa aagctcaaaa acgccgatgc gctgatcgtc gaggcggatg
    21421 tttccaccag cgatacgcct tttgctaatt tgcctgcctg cgaggcgctg gaagagcgca
    21481 ttagcgagga gcaactccaa aacctgcagc acattagcca ggagatgggc atttctccct
    21541 cactcttttc tacccaaccg ctgtggcaaa tcgcgatggt tcttcaggcg acgcaggcac
    21601 aaaaactggg gctgcgggca gaatacggta tcgattacca gctattgcag gcggcgaagc
    21661 aacaacataa acccgtgatt gaactggaag gggctgaaaa ccagattgcc atgttgctcc
    21721 agctccctga caaaggactg gcgctgctgg acgatacgct gacccactgg cataccaacg
    21781 cacggttgct gcaacaaatg atgagctggt ggctgaatgc accgccgcaa aataatgata
    21841 taacgctgcc caatacgttc agtcagtcgc tgtacgatgt gctgatgcat cagcgaaatc
    21901 tcgcctggcg ggataaatta cgcgccatgc cgccggggcg atatgtggtc gcggtcggtg
    21961 cactacacct gtatggagaa gggaatttgc cgcaaatgtt gcgctaaaaa aatggccaat
    22021 atcgctattg gcccgtcaaa gaggaatttc atttttttat tattatgccg tcactttaag
    22081 cgacggtgta actcgattgt cgctcaatct caccatcctg ccaatactct taggcaagat
    22141 gactcttttt tttggaccgc catcaggcgt atgcttgctt cgtttttttg tttaaggcag
    22201 gaaaattacc atgacacccg cagttaaatt actcgaaaaa aacaagattt cgtttcaaat
    22261 ccatacctac gagcacgatc cggctgaaac caattttggc gatgaagtcg tcaaaaaatt
    22321 aggtttgaat ccggatcagg tctacaaaac gctgctggtg gcagtgaacg gtgatatgaa
    22381 acaccttgcc gtggccgtta cgccggtcgc aggtcaactg gatcttaaaa aagtagcaaa
    22441 agcgctgggt gccaagaaag ttgagatggc cgatccgatg gtcgcgcagc gttcgacggg
    22501 atacctggtt ggggggatta gcccactggg gcaaaaaaaa cgtctgccaa cgattatcga
    22561 cgcccccgca caagaatttg ccactattta tgtttccggc ggcaagcgcg gactggatat
    22621 cgaactggcg gcaggcgatc tggcaaagat cctcgatgcc aaatttgctg atatcgcccg
    22681 ccgcgattaa gcattgtgcc ggatgcaaac atccggcact ttcggattac tgccagctca
    22741 cctcaccttt cggttcataa acactcacat ccagcggcga gcttttctgg atatacgctt
    22801 tcagcacttc ggcatcaata aagccggtat tcacatagcc cggtttgtta tcaaggcgcg
    22861 gatatccatc accgccggtg gcattgaagt ttaatgtcgc catacggtaa gttttcgccg
    22921 gatcgaccgg ttcgcctttg attttaaggt cgttcagttt gccgtctttc gccacaaagc
    22981 taacgttggc aaattgcggg taggcacctg aatctggctt catctgcgcg acggcggtca
    23041 ggtaatcaat cacctcttta ccggtcatgt cggcatacac caccacattg ccgaatggct
    23101 gcactttcag cacgttttta tagctgatat cgcctgcttc gatagaatca cgaattccgc
    23161 ctccgctcat caccgcaaag tcggcaccag tgcgatccat ttgggctgcc agaatcaacc
    23221 gccccatatt ggtctgtaca aaacgcactt tgtcacgatc gccttccaga cgaccattgg
    23281 ttttgcctat tttcacttcc agctgcgctt tgcctttgtt ctggaacggt gataacagcg
    23341 agatcatttg ctggttttca gcgatttcag gagtgtaaag cacgcgctcg cttttcccgt
    23401 cttcccaggt cactttcttc ttcaggttca ccggaatcag ctggtagtta accattttca
    23461 tttcgccatt acgaaactca aaatcagccc gtcccacgta tttgccccac tcatgcgcct
    23521 gcacaatcca gatgccgttt tgttgatctg gtttgcatgg cgtacccggc acgtaatcga
    23581 cctgtttttt gttttctgcc gccatgcaga ccggatcttg cgagtgacca ccgacgatca
    23641 tcgccagcga tccggcaggc agcgcgcgtg ccatctccac atcgcccggt gcgttagagc
    23701 cgtgctcacc attatcgtaa tgccccatat gggtcgccgc gataataatg tctggctttt
    23761 ctgtctgttg cagctcctga atcaccagct tcgcttcatc ggcgggctta cgaaattcga
    23821 tatcagtgaa gtattccagg ttaccaattt ttgctgtgtc atcggttgtc agcccaataa
    23881 cggcaatttt cagatcctga cgcttaaaca gcgcccacgg tttaaacagg cgctcgccag
    23941 tacttttctg gtagatattc gcggaaagca acgggaactt ggcccacttt tcctgctggc
    24001 gtaatacggt gagcggatta tcaaattcat gattaccgat cgccatcgcg tcatagccca
    24061 ccagattcat accgcgaaaa tcaggttcgg catcctgtaa gtcagactcg ggcacgccag
    24121 tgttaatgtc gccaccggaa agtagcagca cgctaccgcc ttcagccgca acctctttgc
    24181 ggataccatc caccagcgtt ttttgcgccg ccagaccata ttcgccatat tcattgcgcc
    24241 aaaaatgccc atgatgatca ttggtatgca gaactgtaat tttgtaggtt ttatcctgct
    24301 catacgccag agcagtttca ctcgccagtg taaatgtggt taacagcgct aacgccacgc
    24361 cccgctgcaa taatttcata cttctctccc tgacctgatt tcaacgtgtt gaaaaatagt
    24421 atgacgcaac atcatagaca agtctgcgtc cggtgaagat aaatctgcat ccagattaga
    24481 aattctgtat tcggcgttga gacttccttc agacgacgca tacaggtatg ttagtcgggt
    24541 aataatcaca aataagatca ttctttacat cgcaaccctg aaaaagtgac tttcctatgg
    24601 caatgagtga acaaacccag cctgtggcgg gcgcggctgc gtcaacgacc aaggcccgaa
    24661 catcgtttgg tattttaggt gctatcagcc tctcacatct gctgaacgac atgatccaat
    24721 cgctgattct ggcgatttat ccgctgcttc agtcagaatt ttctctgaca tttatgcaga
    24781 ttggcatgat aaccctcacc ttccagctcg cctcttcgct actgcaacca gtggtcggct
    24841 actggaccga taaatatccg atgccgtggt cgttgccaat tggcatgtgc tttaccttaa
    24901 gtggtctggt gctgcttgcg ctggcgggca gttttggcgc agttctgctg gcagcggcgc
    24961 tggtcggtac cggttcatcg gtctttcatc cggaatcttc tcgcgtagcc cgtatggctt
    25021 ccggcgggcg gcatggcctg gcgcaatcta tctttcaggt cggcggcaac tttggcagtt
    25081 ccctgggacc cttgctggcg gcggtgatta tcgcgcctta tggcaaaggc aacgttgcct
    25141 ggtttgtgct tgcggcactg ctggcgatcg tggtgttggc gcaaattagc cgttggtact
    25201 cggcacagca ccgaatgaat aaaggaaaac ccaaagcgac gataatcaat ccactgccgc
    25261 gcaacaaagt ggtactggca gtcagcattc tgttaatcct cattttctcg aaatatttct
    25321 atatggcgag catcagcagc tattacacct tttatctgat gcaaaaattc ggattatcta
    25381 tccagaatgc ccagcttcat ctgtttgcct tcctgtttgc cgttgcggca ggtacggtga
    25441 tcggcgggcc tgtaggggat aaaattggac ggaaatatgt gatttggggc tctatcctcg
    25501 gcgttgcgcc gtttacgctg attttaccct acgcccgcct gcactggacg ggggttttaa
    25561 cggtgattat tggatttatc ctcgcttcgg cattctctgc cattctggtc tacgctcagg
    25621 agctacttcc gggacgtatc ggtatggttt ctggactctt tttcggtttt gctttcggca
    25681 tgggaggtct gggagcggca gttctggggc ttatcgccga tcacaccagc atcgagttag
    25741 tctataaaat ctgtgctttc ctgccactat tggggatgtt gaccatattc ctgcctgata
    25801 accggcataa agactgattt cattgcagcc aaaggcaaac tttggctgca tcgttttaca
    25861 gtcgccataa gcctttcctc tgttaaaccg ccttctgttt tccgaaccgt gctttttttg
    25921 cagctaattc catttttctg caaaattcaa caattattca taaacatcat caatgaaatt
    25981 gtcataaact attacctgta attttggttt tcccagccaa aaatggacaa taaccaccag
    26041 gaaaaggaga cggaatgcat cacgccaccc cgcttatcac caccattgtt ggcggccttg
    26101 tgctcgcctt tatcctcggc atgctggcca ataaactacg tatttctcct ctggtgggat
    26161 atctgttagc gggtgtgctg gcaggaccat tcactccggg ctttgttgcc gataccaagc
    26221 ttgcgccgga actggctgaa ctgggcgtta ttttgctgat gttcggcgtc ggtttgcatt
    26281 tttcgctcaa ggatttgatg gcggtaaagt ccatcgccat tcccggcgcg atcgcccaga
    26341 tagccgtggc gacgctgctg ggtatggcgc tctccgccgt gctgggctgg tcgttaatga
    26401 ccggtatcgt gttcggctta tgtctttcca ccgccagtac cgtggtgtta ctgcgcgcac
    26461 ttgaagaacg gcaattaata gacagtcagc gtgggcaaat cgccatcggt tggttgattg
    26521 tggaagacct ggtgatggtt ctgacgctgg tgttgctgcc cgcagtggca ggaatgatgg
    26581 aacagggcga tgtgggcttt gccactcttg cagtcgatat ggggatcacc atcggcaagg
    26641 tgatcgcatt tatcgccatt atgatgctgg taggtcgccg tctggtgccg tggattatgg
    26701 cacgcagcgc ggcaaccggt tctcgcgagc tgtttaccct gtcagtgctg gcgctggcgt
    26761 tagggattgc ctttggtgcg gtagagctgt ttgatgtctc ctttgcactc ggtgcgttct
    26821 ttgccgggat ggtactgaac gagtctgaat tgagtcaccg tgccgcccac gatacgttgc
    26881 cattgcgcga cgcgtttgcg gtgctgtttt ttgtctcagt cgggatgttg tttgatccgt
    26941 taattctgat tcagcaaccg ctggcagtgc tggcgacgct ggcaattatt ctgtttggta
    27001 aatcggtcgc ggccctgttc cttgtccgtc tgtttggtca ctcccaacgt acggcattaa
    27061 ccatcgccgc cagcctggcg cagattggtg agttcgcgtt tatcctggcg ggactgggaa
    27121 tggcattgaa tttactgccg caggccggac aaaacctggt actggcaggg gcgatcctgt
    27181 cgattatgct caacccggta ctgttcgcac tactggagaa atatctggcg aagaccgaaa
    27241 cgctggaaga gcagacgctg gaagaggcaa tcgaagaaga gaaacagatc ccagtggata
    27301 tttgcaacca tgcgctattg gtgggttacg gtcgtgtagg cagcctgctg ggggagaaat
    27361 tgctcgcctc tgatattccg ctggtggtga ttgagacgtc acgaacccgt gttgatgagc
    27421 tgcgagagcg tggggtccgc gctgtattgg gcaatgcagc gaacgaagaa attatgcaac
    27481 tggcacatct ggaatgtgca aaatggctga tcctgacgat ccccaacggt tatgaagcgg
    27541 gtgagattgt ggcgtctgcc cgcgcgaaaa acccggatat tgagattatc gcccgcgccc
    27601 attatgacga tgaagtgacg tatatcaccg aacgtggcgc gaatcaggta gtgatgggtg
    27661 aacgtgaaat tgcccgtact atgctggaac tgctggaaac gccaccggcg ggtgaagtgg
    27721 tgacggggta aacggtggtt gccggatgta aaatatgaaa aatatgtagg catgataaga
    27781 cgcgccagcg tcgcatcagg catcgagagc caaatgccag atgcggcgtg aacgccttat
    27841 ctggcctacg aaccgacgat aacttaacga tcccagtaag actcttcctg gctgtcttca
    27901 cgctccggca agccgcgcgt taaacgaggt gaatgctggt tcagtacctg atagctcaca
    27961 cggttagcat atttacacac ctgcgctaac gatgaataag ttaaccaggt gaatttatgt
    28021 ttgctggagt ttggtacgtt gctacgatgg tagctgttgg cggtaatgtc atgcagcaac
    28081 gctgccaatg cgccatcccc cgctccatta gtgttcatga ttttttccgg cccgcccatg
    28141 tacggcgcaa tgtgcgaata gacacgcagc ggattctggc aatccttgtg gcgcatggcg
    28201 cggctaaact catactggtt gaattccgct atagcgcccg gcagcagcgg atgctgggtt
    28261 ttacgtttcg cttcgtcttc ggtaaagccc gccatataca agccgattgg cccggcggtg
    28321 cacagcacca gctctaccca gtccagcgcc ttgtcagatg ccaacaacgg atcgctttct
    28381 ccggtcaacg cttcggcttc atcttcgttc atcgcaagga tagaaacgtg gtctttgagg
    28441 aattgctgcc accactgcgg attctcggca atgacaaact tggtgcccag cgtcagcacc
    28501 accggtacgt tatatttctt cgcgtactca atggctttca tggttgcttc cggcatgggt
    28561 tcacccggct tgcaacgcac cagatatgag gtgagaacca gtgccgaggc tccggcaatc
    28621 acatcttccg gaatgctttc agcccgcagc tggttcatgt ggcctggact gatagcaaag
    28681 gtacgttccc cggactcgcc aatcagcgta aagcaacgac caatcggacc atccacgcct
    28741 tgtagatagt taagatcggt acggctggaa gtgttacaca ggtaacgata ggcataactg
    28801 ccaatttcaa tattgctgca catgacgccc agcagcaccg aacggtcgtc cgcgagcacc
    28861 gagtagttgt gcatggtgtt accaatggtg ccacccgcaa actgatgggt aatcaggttt
    28921 ttctgtttta gctcctgata aagcacttcg gctacgtcat cctcaatcac cagtgaatgc
    28981 ccggcgctta atccataacg ctcaatgaat tcatcatcca ctttcgcttc aatatcgacc
    29041 agcgtctggt caatacctac gacccaggcg gcgctggttt cgttttctgg ctggaattgc
    29101 tgaagcagcg gatcgcgtgc atttacaggg aagtaatgtt tggatttacg tttaccggga
    29161 aatttcatgg ttgtttttac gggtggttaa ctgagcggcg aatgttaaca caatctgacg
    29221 ggagcgcgcg agacgcagcc agtttcaata tcagcaggcg ggatcgcatt ttcatggcag
    29281 tggttcctta caatgacgta atttgaaagg agtttttgta tgaagccgga aaacaaacta
    29341 cctgttctgg accttatttc tgctgaaatg aagaccgttg tgaatactct tcagccggat
    29401 ttaccgtcct ggcccgcaac gggaacgatt gctgagcaac gacagtatta cacgcttgag
    29461 cgccgattct ggaatgcggg cgctccagaa atggcaacca gagcttacat ggttccaaca
    29521 aaatatgggc aggtggaaac acgtctcttt tgtccgcagc cagatagccc ggcgacgcta
    29581 ttttatttgc atggaggcgg ttttattctc ggcaatctcg atacccacga tcgcatcatg
    29641 cgcctgctgg caagctacag ccaatgtacg gtgattggta ttaattacac cctttcacct
    29701 gaagcgcgtt ttccacaagc gatagaggaa attgtggctg cttgttgtta tttccaccag
    29761 caggcggagg attatcaaat caatatgtcc cgcattggct ttgccggtga ttccgcaggt
    29821 gccatgctgg cgctcgccag tgcgttgtgg ttgcgtgata aacagatcga ttgcggtaaa
    29881 attgcgggtg ttttgctgtg gtatgggctt tacggattac gggattccgt gactcgtcgt
    29941 ctgttgggcg gtgtctggga tggcttaacg caacaggatt tgcagatgta tgaagaggcg
    30001 tatttaagca acgacgcgga ccgcgagtca ccgtattact gtctgtttaa taatgatctc
    30061 actcgcgaag ttccgccctg ttttattgcc ggggcggagt tcgatccgct gctggatgac
    30121 agccgtctgc tttactagac gttagcggcg catcagcagc cctgtgagtt caaactctac
    30181 ccaggtacgc tgcatgcctt tttgcattat tcacggatga tgaaaaccgc cgacgaggct
    30241 cttcgcgacg gcgctcagtt ctttaccgct cagctttagc gatacgcggc aacaagatta
    30301 gccatcattt cgatatgttc cggcgtggca ttaagcgctg gaatatattc atattttttc
    30361 ccgcccgcac cgaggaagac ctcacggttt tgctcggcaa tctcttccag cgtctccaga
    30421 caatccgcag caaagcccgg gcacatcacc tgtatatgac ctacgccttt ttctccgagc
    30481 attttcagcg tttcgtcggt ataaggcatc agccagggtt cccggccaaa gcgcgactga
    30541 aaggtcatca tcactttttc cggtaccatc cccagtgcgg aagccagttc gcgagtcgtt
    30601 gtgcggcaac gttgcgggta atcatcgcct tcatctgcat aacgctgggg aatgccatga
    30661 taagagagca gcagcagatc cggttcgcca tgtttggcaa aagaagcgcg tacgctgttc
    30721 gccagtgcat taatgtaatc gtggttatcg gcgtaatcac gaataaacga tatccccgga
    30781 atgctacgtt tgcgcgccag aatgcgtgcc agttcatccc ataccgcacc gaccgtagaa
    30841 caggagaatt gcggataaag cggcagcacc acaatatgat ctacatgctc tgccaggagt
    30901 tcatcgacgg agctttccag tgatggcgag ccgtagctca tgcccagcgc cactggcgta
    30961 tccggtaaac gttgtgccaa cgcctgctgt tgctggcggc tgtaaaccat cagcggcgag
    31021 ccgtcttcca tccagacaga ggcgtacagc ttcgccacac gcggcgagcg cagcggcaaa
    31081 atcacgccgc gcagcaacgg ccaccataac aaccgtgagg tatcaaccac gcgtctgtcg
    31141 cttaaaaatt gtttcagata gcgttttacc gcttcaggtg tgggggcatc gggcgtaccc
    31201 aggtttgcca gcaggatacc ggttttagtc tgacgcatta ccgcctctta tcgattcaac
    31261 ttgttgataa ttgtagcgga aaagtgagga agaagaacta attgctgtga gaggtattgc
    31321 ctgaggtgga taagcagacc tgcccgaaag cagatctgct gcggcttgat tagccgagga
    31381 ttttttccag atcagcgcga acttcagcaa cctgcttggt accgtcaact ttcgcgtatt
    31441 tggtgttacc cgcttccgct tctttggagt agtagccgat cagcggtgca gtcatctgat
    31501 ggtattcaac cagacgttta cgcacggttt cttcctgatc gtctttacgg gtagtcagtt
    31561 cttcaccggt aacgtcgtct ttgccttcta ctttcggcgg attgaattta acgtgataaa
    31621 cacgaccaga cggcgcatga acgcggcggc ctacgatacg atcaacaatc agttcgtccg
    31681 gtacgtcgaa ttccagaacg taatcaacat tgatgcccgc ttctttcatc gcgtctgcct
    31741 gcggaatggt acgcgggaag ccgtccaaca ggaaaccatt acggcagtct tcctgagcaa
    31801 tgcgctcttt aaccagcgcg atcaccagtt cgtcggtgac cagtttgcca gcatccataa
    31861 tgtcttttgc ttgtttaccc agctcggagc cagatttgac cgcagcacgc agcatatcgc
    31921 cagtggagat ttgcggaata ccatatttct ccatgatgaa ctgagcctga gtccctttcc
    31981 ccgcgcccgg agcgccaagc agaatgatac gcattgcgaa aatcccctta aaatgtgtcg
    32041 atttttttga aaaagcgata aacgatacca ccaacaggcg attgcctcaa gaaaggcacc
    32101 tgaggctgaa acggttaatg gcgagataat ttgcggaaag tacagatgat gaaaagaaaa
    32161 atgccggatg acacaaaggt catccggcat gacatcagga aaccagcagc tgattcatac
    32221 gacgaataaa caggttcgga tcttccagcg tgccgcgttc tgccagcagc gcctgatcca
    32281 gcagcagttc tacccactcg ctgaacttgg cttcatcttc agtatctgcc gcacgtttca
    32341 ccagtacgtg atccgggttc agttcgaaga tgtatttcac ttctggcact ttctggcccg
    32401 ccgcagcgaa cagtttcgcc atctgagtgc tcatttcgtc cgcgtcggtc gaaacgatcg
    32461 ctggcgtatc ggtcagacgg tgagtcagac ggacatcttt cacgcgctcg ccgagcagag
    32521 ctttcacgcg gtcgatgaac ggagtcagtg ctttctccgc ttctttcgcg ctctcatcaa
    32581 cttcgtcagc cagtttttcc agcgactcgt caactttaga caccgactgg aacggtttac
    32641 cgtcgaactc agtcagatag ttcatcatcc actcatcgat gcggtcggaa agcagcagaa
    32701 cttcgatgcc tttcttacgc agcagttcca ggtgcgggct gctcttcgct gccgcatagc
    32761 tgtctgcagt gatgtagtag attttctcct gcccttcttt catgcgggaa acgtagtctt
    32821 ccagagacac ggtctgcgca gaagaatcgg tataagtaga agcaaaacgc agcagtttgg
    32881 cgatcgcttc ctggttagcg aaatcttccg ccggaccttc tttcagtacc aggccaaact
    32941 gttgccagaa ggtctggtat ttttccgcgt cgtctttcgc cagtttttcc agcatttgca
    33001 gcacacgctt ggtcagcgca ttgcgcaggt tacgcgttac cgtgctgtcc tggaggattt
    33061 cacgggaaac gttcagcggc agatcgctgg agtcaatcag accacgcacg aagcgcagat
    33121 agttcggcat gaactgttca gcgtcgtcca tgataaacac gcgttgaacg tacagtttca
    33181 ggccgtgttt atgatcacgg ttccacatat cccacggagc ctgggacggg atgtacagca
    33241 ggctggtgta ctcctgctta ccttcaacgc ggttgtggct ccaggtcagc ggatcgttaa
    33301 agtcgtgggc gatgtgtttg tagaactctt tatactcttc atcggtgatt tccgacttgt
    33361 tacgagtcca cagcgcctgc gctttgttga tttttgccca ggagataacg gtttcgccat
    33421 ctttctcttc gcgtttttcg atctctaccg gcagcgcgat atggtcggag tatttgctga
    33481 tgatggaacg cacgcgccag tcatcgagga actcgtcttc gccttcacgc agatgcaggg
    33541 tgatttcagt accacgatct tctttggtga tatcggcaac ggtatattca ccttcgccag
    33601 ccgattccca gaagacgccg ttttctggtt tttcgcctgc cgcgcgagta cgcacggtca
    33661 ctttgtcggc cacgataaac gcagagtaga aaccaacacc aaactgaccg atcagctggc
    33721 tgtctttcgc ttggtcagaa cccagggatt cgaggaatga tttggtaccg gatttagcga
    33781 tagtccccag atggtcaatc acttcgtcgc gggtcatccc cacgccgtta tcggagatgg
    33841 tcagcgtgcg cttgtcttta tcgaaagaga cacgaacgcg cagttcgcca tcaccttcgt
    33901 acaggtccgg gttagatagc gcacggaaac gcagcttgtc cgccgcatcg gaggcgttag
    33961 agataagctc acgcaggaag atttctttat tggaatagag agaatggatc atcaggtgca
    34021 gaagctgttt cacttctgac tgaaaaccac gagtttcttg tcctttcatg taggtctacc
    34081 tcaataatgc cattttaagg ttaaaaacag gatgagggag agatggggac aagggagaat
    34141 aatttcaagc gagagcaggt gctcctgctc tcgtttgctt aaaaacgaat cttatgacgc
    34201 ccggcgaggg agtgtgacag cgtggtgcca tcgaccattt ccagttcgcc gccaaccggt
    34261 acgccatgag cgattcggct ggcttccacg tcatattgcg cgcaaagctc ggcaatgtag
    34321 ttagcggtag cttcaccttc aaccgtggga ttggtggcga ggatcacttc agtgattttt
    34381 tcctctgcca gacgctgttc cagacgatca agcccgatat catccggacc gatgccgtcc
    34441 agcggtgaca gatgccccat caacacaaaa taacgaccag aaaactgccc cgtctgctca
    34501 atggcgtaga tgtccgccgg actctccacc acacagattt gaccgttttc ctgacgacgc
    34561 ggattcgaac agatgttaca gacttcctgt tcggtgaaag tgcggcaatc ggcgcagtgg
    34621 ccgatttccg acatcgcccg ggtgagcgcc tgcgccagac gcatcccgcc gctacgatcg
    34681 cgctgaagca gcgtgaacgc catacgctgc gccgacttcg ggccaacgcc cggcagacag
    34741 cgcagtgctt ccataagctg tgttaacagc gggctggttt gcatcagaac ggcatcttaa
    34801 agccaggcgg cagctgcatt ccggaggata cagaggccat tttttctttc tgcgtttctt
    34861 caatacgacg tgctgcgtcg ttgaatgctg cagccaccag gtcttccagc atctctttgt
    34921 cgtcttccag caggctcggg tcgatctcta cgcgacggca gttgtgtgca ccgttgatgg
    34981 tcacttttac cagacctgcg ccagattcgc cggtaacttc cagctgcgcg atctcttcct
    35041 gcattttctg cattttttct tgcatctgct gggcttgctt catcaggtta cccagaccgc
    35101 ctttaccaaa cataggtttc tctctcaatc acgttaagga tgacgaacgt aagctgtgct
    35161 tacgatcaaa tggggcggat actttcttca tccagctccg catcgaaaaa ccgacgcagg
    35221 gtctgaatat tattatccgc aataatggac tcgcgcgcct gcgcaagttt ttcttcgtat
    35281 atcgcctgac gccactccag cggcgtacgc accgcgggat tatcatcttc aacgatagtc
    35341 agttcaaccg ttgaaccttt taacatgctc aacgcttcag ccagtttttg ctgtgcaccg
    35401 cggttgttca aatgccgctg agaggagcgc aaatgcagac atactgcgtt gtcgctctcc
    35461 tctttccagg catttaacgc cacctgttcg accagttttg gtagcgaaag ttggctcacc
    35521 tgtgccgccc acgggtcgcg ctcaatggct tctgccgcta gcttcaccgc cagttccggc
    35581 gttttttcat gttccagcgc ttttttcagc gccttcggcg tggcgaccac ttctttttgc
    35641 tgcatcaccg gagtggtcgc cttccagcga tacgcttctt ttttggctgg cgctttttcc
    35701 agcgccgatg gcaccggacg cgcctgaacg cgatcggtga ccgaagccag tctttccagc
    35761 gcagcgttat tcaccggccg cgcgcgggta gcggctgccg gttcactctt ttttgctttg
    35821 gttgctccct gcacgcgctg caactgctgg cgcgccgcca gcacctggct ggtggtttcc
    35881 gggagcggta cagtcggtgc ctgctgcggc gctgattgcg gcggcaccag ggttggcgtc
    35941 attactgccg ttggcgcgac gggtgcaaag gactggcgtg gcacttctgg ctcaggcagc
    36001 ggcatacgcg gatggaacgc cagcgcgcgc agcagcgtca tctcaacgcc catgcggcgg
    36061 tccggcgcat acggtaattc tttgcgacca atcaacagcg tctgatagta aagctgaata
    36121 tccgtcggcg gtatggtgcg cgccagttca cgcatccgca gctcgatggc ggccatgtcg
    36181 ttgccaagtg cagcaggcga aagttgtacc atcgcaatac gatgcaacaa gccgagcatt
    36241 tccaccagca acgcttccca ctcgatacca cgggcagcgg cttcattaat cagtgccatt
    36301 acgcgctcgc cgttgccctc gaccatcgct tcaaccagcg acagcgcctg atcgtcgtca
    36361 agcgtaccca gcatcgcact gaccgcctgg gttgaaacct ggccgtcacc gctggcaatc
    36421 gcctggtcgg tcagacttaa ggcatctcgc aggctgcctt cagcggcgcg cgccaacaat
    36481 tgcagcgccc gcggctcgtg agcgatatgt tcttcattga ggatgtgctc aagctgatgg
    36541 cgaatttgct cgacatccag cgccttgaga tgaaattgca gacagcgtga caaaatcgtc
    36601 accggcaatt tctgtggatc ggtcgtcgcc agcagaaact taacgtgctc cggcggctct
    36661 tcaagggttt ttaacagtgc gttaaagctg tggcgcgaca gcatatgcac ttcgtcgatc
    36721 agataaactt tgaaacgacc acgcgccgga gcgtactgga cgttatccag caggtcgcgg
    36781 gtatcttcaa ctttggtgcg cgaggcggcg tcgatttcaa tcagatcgac aaagcgcccc
    36841 tgctcgattt cacgacagtt atcgcacacg ccgcacggcg tcgcggtaat gccggtttcg
    36901 cagtttagcc ccttcgccag cagtcgggcg atagaggttt ttccgacgcc acgggtgccg
    36961 gaaaaaagat aagcatgatg aatacgccct aacgacaagc cgttcgccag tgcggtcagc
    37021 acatgttcct ggccgacgac gtcagcaaag gtttgtgggc gccattttcg ggctaaaacc
    37081 tgataactca ttggcaggct ctgaaacgct ggaaggtgga ttcacgaagg ggtaatgcta
    37141 acacagcccc gtcagaacgg cgaggctgaa tgcacatctt gtgctgtccg tagcgtgggc
    37201 agcacaagac tggcgataat taatggcccg ggaacgggac aaggctgtag ctggtaatgc
    37261 cctgtttttc gagacgctgt tcgccgccga gatcaaacag gttgataatg aacgcagcgt
    37321 cagccacttc accacccaga cgacggatca gtttaacggt cgcttcgata gtgccgccgg
    37381 ttgccagcag atcgtccacc accagaactt tgtcgcccgg tttgatggca tcaacgtgga
    37441 tttccagctg atcggtgccg tattccaggt cgtaattttc actgatggtt tcacgcggca
    37501 gtttgcccgg tttacggacc ggtacaaagc caacgcccag acccagagct accggagcgc
    37561 caaacaagaa gccacgcgct tcggtgccga caactttggt aatgcccgca tttttgtaac
    37621 gctcaaccag caagtcgatg ctgagagcgt aagctttcgg gtcttccagt aagctggtga
    37681 catcgcggaa aagaatgccg ggttttgggt agtcctgaat gcttttgatg ctatttttga
    37741 gatactcaag ctgctgtgca gtcgcggtca taagtgtatg cctgcttgtt acggtggtac
    37801 tcacggcgcg ttttaaacgt atcaaaagta actgttgttt aacctttgac cgcctgcgcc
    37861 tgtgctcgaa aacgccagaa tgtactggct gtacgcaata attgcaactg cgattgttgt
    37921 gcttcagtgc ttttcttgct tttcatcaat caccggaatt cgccacatat agaaaagcaa
    37981 acaggcgaga attaccagca acatgatgcg cacccatggc atctggacga accacagaga
    38041 aatggcaaac gtgagcaaaa taagcaaaat cgcccgcggt ttgacgccgc gcggcatcgc
    38101 atgatgtttc tgccagaaac gtagatagct gccaaaccat gagcggtaca gcaaccaggc
    38161 gtgaaagcgc ggggaagaac gggcaaagca ccaggccgcc agcaggataa acggcgtcgt
    38221 cggtaatacc ggtaatacca cgcccagcgt acccagcact accgccagcc agccaatgat
    38281 gattaaaatg attcgttgca tattgagtgt tgtcagctta catagtggct actttagcat
    38341 aacaattatc attttcattg aggtcttatc gtgaaaaccg ccctgctgct ggaaaaactg
    38401 gaaggacagc tcgctacgct gcgtcagcgt tgtgccccgg tgtcacagtt cgccacgcta
    38461 agtgctcgtt tcgacaggca tctttttcag actcgtgcga caacactaca gtcttgtctc
    38521 gacgaggcgg gcgataatct ggctgcgctt cgtcatgcag ttgagcagca acagctgccg
    38581 caagtggcct ggctggcgga acatctggcg gcacaactgg aagccatcgc gcgtgaagcc
    38641 tccgcctggt cattgcgcga gtgggacagt gcgccaccga aaatttcccg ctggcagcgt
    38701 aaacgtattc agcatcagga ttttgagcgg cggctacgtg agatggttgc cgaacgcaga
    38761 gcccgcctgg cgcgggtgac cgatctcgtg gaacagcaaa cgctgcatcg tgaagtggaa
    38821 gcctatgaag cgcgcctggc acgctgccgc catgcgctgg aaaaaatcga aaacaggcta
    38881 gcgcgcttaa cccgctagca atggaaagag tatgtcactt gaaaatgccc ctgacgatgt
    38941 caaactggct gtcgatttga ttgtgctgct ggaggaaaat cagatccctg ccagtaccgt
    39001 gctgcgcgcg ctggatattg taaagcgtga ttatgaaaag aaattaacgc acgatgatga
    39061 ggcggaaaag tgagaaataa atggggccgc taaatgtccc ctaagattgc gggcaaacgc
    39121 aaaactgcct gatgcgctac gcttatcagg cctacaccgc tcctgcaata tattgaattc
    39181 ttgcggtttc gtaggccgga taaggcggtc acgccgcatc cggcatgaac aacgcgcact
    39241 ttgtcagtaa tctcggggca actaaatgtc gccccctttg gttattaccc aaccgctggt
    39301 gtcggatcgt cgcctttgta gtcgcgtttt acttccgtca cctcatcgcc cttctcgttg
    39361 tgcagatgca cttcaagctg gttaaaggca atgttgatgt cgttttcacg gcacagctga
    39421 tcgatagtac ggttcagctc atcgacagta cggctacggt cacgcagttc acgcacatac
    39481 agacgcagct catgatccaa cgtgctggca ccaaatgccg taaagaagac ttccggcatt
    39541 ggttcgtgca tcacccttgg gtgctcagtc gccgccttca gtaacacttt acgcactttt
    39601 tccagatcgg agccataggc cacgccgaga cggatcacca gacgcgtagt agtgtcagtc
    39661 aacgaccagt tgatcagacg ctcggtaaca aacgctttgt tcgggatgat cacttctttg
    39721 cgatcgaaat cggtaatcgt tgtcgcacga atacggatct tacttaccgt ccccgagaag
    39781 ctaccaatgg ttaccgtatc gccaatacgc accggacgtt cgaacagaat gatcaaaccg
    39841 gagacgaagt taccgaaaat ttcttgtaaa ccaaaaccaa gacctacgga taatgctgcg
    39901 gccagccact ggagtttatc ccaagagacg cccagcgatc cgaacaccgt catcgcacca
    39961 acggcaataa tgatgtagtt aaggatggta gtaatggcat acgacgcgcc ctggcgcata
    40021 ttcagtcgcg agagcaccag cacttccagt aaaccaggca ggttgcgaat caacgcccag
    40081 gccaccattg aggcgataat cgcaaacaac agactgccca tggtgacgtt tttcaccacc
    40141 gcagcgccag cttcagtgcc gttgtaatgc cagagcgtga tgctgtcgag atagctgaac
    40201 acggtgatca aatcggacca aattgcccag aacatgacac cgaacagcgc aaacatcagc
    40261 aacatggtaa tacgcagcgt ctgctggtta acttgctcca gtgcaatggt gggttcttcc
    40321 ggcggttcgg caccttctgc gccctctttc accagattct gccgacgcgc cagcgcacga
    40381 cgccaggcga tacgccgcgc cgctacgctt aagccgcgca ataccgtctg gtagagcaag
    40441 ttccagatga tcaccagata aacggtttca atccaacgcc cggagaggcg cagcgtggtg
    40501 tagaaataac ccgtggcagt cagcaccatc aatgcaatcg ggataatcga cagcacggta
    40561 atggtgacta gtcgcatggt gtgcgactct ttatcacgcc agctttcgcg gcacattggc
    40621 cataccagga aggcaatcag cagcaggttg aagaaaatca tcgcctgccc cagcacatca
    40681 tccatcagat gcagcgggga aagttctgcc accacagacc agaaatggat aggcagcaac
    40741 gcgagactga tgcggacaat ttgccgacgc cagtggctgg tctgctgctc cggcatgccg
    40801 aagtgacgca cggcaacgcc atttttctcc agtaccttcc agcacaggcc aaacaccagc
    40861 cagaaaatcg ccagtttttt actgaacgac catagcagtt cgctgatgtt gagctgcatg
    40921 gtcaacagaa tcaggccaac cgcgagaata atcaggcaca ccggcagcgc gcggatcagg
    40981 tcgataagga tcgcttttgg tgtgttgagc tggctatcgt tacgcaggga acccacagcg
    41041 gaagccagtt tctgttgata cgctttcagc cagcccagac gccagtggat cagcccggca
    41101 atcaacagca gcggcaaacc ggcgaggaaa gcgataaaaa cggcaggcca ggctttttcc
    41161 cagttcaccg tgattttcat cgacttaaat tcatctttca ggctttgcgg gaacgctttg
    41221 atccagtccc agtccattgg acggttactg ttcacccaaa agatttgctg agtcaggatg
    41281 gatttcaggt ttttcgatac gctcattaac tgctgctggt tgatttgcag gttaatggcc
    41341 atcatcagct ggttacccaa ctgtttgttg agttgatcca gcaattcgcg acgcatatca
    41401 accacttgca ataacgcatc gtgaacttcg ctgttgactt cgttggtgtg accttcttcc
    41461 agtttgttga cgaacgcatc gctctggaag agtgcatcac gctgctggtt aacttcaaac
    41521 tgttcgagac gcaaatccgc gatgcggttg gtcatgtttt ccagttcatc cgccgagggc
    41581 agcgtttgtt gttgctggta aaggatacga gacaacagca ggctgccctt caggacggca
    41641 atctgctctt taatattgcg ttccgattgc agcgcccgct ccagccagtt tttgacttta
    41701 atgttttgct gcatcaactg attaccgttt tcagtcgcgg taatcagacg ctgacttaac
    41761 tgctggttaa tttccagttc ctgtttcacc agcggattag cctgaatacg cgcggcttca
    41821 tccggggaga cggcttcctg cgccgttttt tcagttaaag tcaggcgctt gctgtttacc
    41881 gcttcttgca acagctgcaa ctggtgctcc agacgggcgc tgttcgccgt cacgtaatca
    41941 cgttgctttt gcaaggtatc ctgcaagacg gtgttccctt ccaggctttt acgctgctgg
    42001 tcaatctccg cattcagcaa cgcctgctga gcctgcatta acactttctg gctgggacgt
    42061 aaggcagtct cgccgacatc agtcccatcc agacgactgc gaatttgttg cagctgctgc
    42121 gaagcgttat acatcgcatt ttgcacgcgt tcgggctgcg tctgtaacga aaccagctgg
    42181 ctgttataag acgccagatc gttttgtgcg ttttgcaaat cgtccagcgc ctgggcaacg
    42241 cgagtttcca gctggcgcaa cgaaagcgta ctcagaattt tgcgcgtttc ttcgtcgtta
    42301 tcgacatcgc taagtgctgt taacgccgcg gtcgcctggc gcattttttc cggcgcttca
    42361 gcgacttttt gccgtagctg aactgtctct tcttttatgc gatcgatttt atcgagggtg
    42421 gctaatgtat ctgtcagatc ctgctgcacc agtttgtcct gagcagaaag atctttttgt
    42481 ttatttagtg agtcaagttg cgcctgcagg tccgcttttg tcggcagatc accattcgat
    42541 gacgcccgcg caaacgccgt gttctggcat aacaagacaa aaacaaaagc aataaatgct
    42601 gaaaaaacaa aatgccgtga tcgtttgtaa tactggaaca tagtcatgat gaatgaaggt
    42661 ttctgaacct gaagaacgac ctgaaaaagt caaaccgcaa gaatatcacg acgcagtgaa
    42721 ccagaatagc aacgacgaaa atgtccagga aaaatcctgg agtcagattc agggttattc
    42781 gttagtggca ggattacgaa gcgtggggca caggagatac atctccagta agatggcgac
    42841 gtaatcgcgg gcttcttttt taagatcaaa agattgcggg gcaaagagcc agttttccat
    42901 caggccggaa atatagccgc gcataataat tgctgcccga cgcgtcatta aatccgcagg
    42961 caacattttc gcttcaatac aatgttttaa cgtttgttct atacggtcat aactttccag
    43021 acagagatta cgttgtgcct gttgcacaac agccatttct ccgacaaatt cgcatttgtg
    43081 gaatataatc tccatcaata atcgacgtcg ttcttctgtc accgtggatt caagaacatg
    43141 aattaatatc tctcttaata ctgagagtgg atcgccaggg aattttgcct gatactcaag
    43201 ctctagttca ccaatattgg attctgacag ttcccagatc tcactgaaca aatccgactt
    43261 gtctttaaaa tgccagtaga ttgcaccgcg cgtaacgcca gctgcttttg caatctcgcc
    43321 cagcgaggtg gatgataccc cctgctgtga gaaaagacgt agagccacat cgaggatgtg
    43381 ttggcgcgtt tcttgcgctt cttgtttggt ttttcgtgcc atatgttcgt gaatttacag
    43441 gcgttagatt tacatacatt tgtgaatgta tgtaccatag cacgacgata atataaacgc
    43501 agcaatgggt ttattaactt ttgaccattg accaatttga aatcggacac tcgaggttta
    43561 catatgaaca aaaacagagg gtttacgcct ctggcggtcg ttctgatgct ctcaggcagc
    43621 ttagccctaa caggatgtga cgacaaacag gcccaacaag gtggccagca gatgcccgcc
    43681 gttggcgtag taacagtcaa aactgaacct ctgcagatca caaccgagct tccaggtcgc
    43741 accagtgcct accggatcgc agaagttcgt cctcaagtta gcgggattat cctgaagcgt
    43801 aatttcaaag aaggtagcga catcgaagct ggtgtctctc tctatcagat tgatcctgcg
    43861 acctatcagg cgacatacga cagtgcgaaa ggtgatctgg cgaaagccca ggctgcagcc
    43921 aatatcgcgc aattgacggt gaatcgttat cagaaactgc tcggtactca gtacatcagt
    43981 aagcaagggt acgatcaggc tctggctgat gcgcaacagg cgaatgctgc ggtaactgcg
    44041 gcgaaagctg ccgttgaaac tgcgcggatc aatctggctt acaccaaagt cacctctccg
    44101 attagcggtc gcattggtaa gtcgaacgtg acggaaggcg cattggtaca gaacggtcag
    44161 gcgactgtgc tggcaaccgt gcagcaactt gatccgatct acgttgatgt gacccagtcc
    44221 agcaacgact tccttcgcct gaaacaggaa ctggcgaatg gcacgctgaa acaagagaac
    44281 ggcaaagcca aagtgtcgct gatcaccagt gacggcatta agttcccgca ggacggtacg
    44341 ctggaattct ctgacgttac cgttgatcag accactgggt ctatcaccct acgcgctatc
    44401 ttcccgaacc cggatcacac tctgctgccg ggtatgttcg tgcgcgcacg tctggaagaa
    44461 gggcttaatc caaacgctat tttagtcccg caacagggcg taacccgtac gccgcgtggt
    44521 gatgccaccg tactggtagt tggcgcggat gacaaagtgg aaacccgtcc gatcgttgca
    44581 agccaggcta ttggcgataa gtggctggtg acagaaggtc tgaaagcagg cgatcgcgta
    44641 gtaataagtg ggctgcagaa agtgcgtcct ggtgtccagg taaaagcaca agaagttacc
    44701 gctgataata accagcaagc cgcaagcggt gctcagcctg aacagtccaa gtcttaactt
    44761 aaacaggagc cgttaagaca tgcctaattt ctttatcgat cgcccgattt ttgcgtgggt
    44821 gatcgccatt atcatcatgt tggcaggggg gctggcgatc ctcaaactgc cggtggcgca
    44881 atatcctacg attgcaccgc cggcagtaac gatctccgcc tcctaccccg gcgctgatgc
    44941 gaaaacagtg caggacacgg tgacacaggt tatcgaacag aatatgaacg gtatcgataa
    45001 cctgatgtac atgtcctcta acagtgactc cacgggtacc gtgcagatca ccctgacctt
    45061 tgagtctggt actgatgcgg atatcgcgca ggttcaggtg cagaacaaac tgcagctggc
    45121 gatgccgttg ctgccgcaag aagttcagca gcaaggggtg agcgttgaga aatcatccag
    45181 cagcttcctg atggttgtcg gcgttatcaa caccgatggc accatgacgc aggaggatat
    45241 ctctgactac gtagcggcga atatgaaaga tgccatcagc cgtacgtcgg gcgtgggtga
    45301 tgttcagttg ttcggttcac agtacgcgat gcgtatctgg atgaacccga atgagctgaa
    45361 caaattccag ctaacgccgg ttgatgtcat taccgccatc aaagcgcaga acgcccaggt
    45421 tgcggcgggt cagctcggtg gtacgccgcc ggtgaaaggc caacagctta acgcctctat
    45481 tattgctcag acgcgtctga cctctactga agagttcggc aaaatcctgc tgaaagtgaa
    45541 tcaggatggt tcccgcgtac tgctgcgtga tgtggcgaag attgagctgg gtggtgagaa
    45601 ctacgacatc atcgcagagt ttaacggcca acctgcttcc ggtctgggga tcaagctggc
    45661 gaccggtgca aacgcgctgg ataccgctgc ggcaatccgt gctgaactgg cgaagatgga
    45721 accgttcttc ccgtcgggtc tgaaaattgt ttacccgtac gacaccacgc cgttcgtgaa
    45781 aatctctatt cacgaagtgg ttaaaacgct ggtcgaagcg atcatcctcg tgttcctggt
    45841 tatgtatctg ttcctgcaga acttccgcgc gacgttgatt ccgaccattg ccgtaccggt
    45901 ggtattgctc gggacctttg ccgtccttgc cgcctttggc ttctcgataa acacgctaac
    45961 aatgttcggg atggtgctcg ccatcggctt gttggtggat gacgctatcg ttgtggtaga
    46021 aaacgtggag cgtgttatgg cggaagaagg tttgccgcca aaagaagcta cccgtaagtc
    46081 gatggggcag attcagggcg ctctggtcgg tatcgcgatg gtactgtcgg cggtattcgt
    46141 accgatggcc ttctttggcg gttctactgg tgctatctat cgtcagttct ctattaccat
    46201 tgtttcagca atggcgctgt cggtactggt agcgttgatc ctgactccgg ctctttgtgc
    46261 caccatgctg aaaccgattg ccaaaggcga tcacggggaa ggtaaaaaag gcttcttcgg
    46321 ctggttgaac cgcatgttcg agaagagcac gcaccactac accgacagcg taggcggtat
    46381 tctgcgcagt acggggcgtt acctagtgct gtatctgatc atcgtggtcg gcatggccta
    46441 tctgttcgtg cgtctgccaa gctccttctt gccagatgag gaccagggcg tatttatgac
    46501 catggttcag ctgccagcag gtgcaacgca ggaacgtacg cagaaagtgc tcaatgaggt
    46561 aacgcattac tatctgacca aagaaaagaa caacgttgag tcggtgttcg ccgttaacgg
    46621 cttcggcttt gcgggacgtg gtcagaatac cggtattgcg ttcgtttcct tgaaggactg
    46681 ggccgatcgt ccgggcgaag aaaacaaagt tgaagcgatt accatgcgtg caacacgcgc
    46741 tttctcgcaa atcaaagatg cgatggtttt cgcctttaac ctgcccgcaa tcgtggaact
    46801 gggtactgca accggctttg actttgagct gattgaccag gctggccttg gtcacgaaaa
    46861 actgactcag gcgcgtaacc agctgcttgc agaagcagcg aagcaccctg atatgctgac
    46921 cagcgtacgt ccaaacggtc tggaagatac cccgcagttt aagattgata tcgaccagga
    46981 aaaagcgcag gcgctgggtg tttctatcaa cgacattaac accactctgg gcgctgcatg
    47041 gggcggtagc tatgtgaacg actttatcga ccgcggtcgt gtgaagaaag tttacgtcat
    47101 gtcagaagcg aaataccgta tgctgccgga tgatatcggc gactggtatg ttcgtgctgc
    47161 tgatggtcag atggtgccat tctcggcgtt ctcctcttct cgttgggagt acggttcgcc
    47221 gcgtctggaa cgttacaacg gcctgccatc catggaaatc ttaggccagg cggcaccggg
    47281 taaaagtacc ggtgaagcaa tggagctgat ggaacaactg gcgagcaaac tgcctaccgg
    47341 tgttggctat gactggacgg ggatgtccta tcaggaacgt ctctccggca accaggcacc
    47401 ttcactgtac gcgatttcgt tgattgtcgt attcctgtgt ctggcggcgc tgtacgagag
    47461 ctggtcgact ccgttctccg ttatgctggt cgttccgctg ggggttatcg gtgcgttgct
    47521 ggctgccacc ttccgtggcc tgaccaatga cgtttacttc caagtaggac tgctcacaac
    47581 cattgggttg tcggcgaaga acgcgatact tatcgtcgaa ttcgccaaag acttgatgga
    47641 taaagaaggt aaaggtctga ttgaagcgac gcttgatgcg gtgcggatgc gtttacgtcc
    47701 gatcctgatg acctcgctgg cgtttatcct cggcgttatg ccgctggtta tcagtactgg
    47761 tgctggttcc ggcgcgcaga acgcagtagg taccggtgta atgggcggga tggtgaccgc
    47821 aacggtactg gcaatcttct tcgttccggt attctttgtg gtggttcgcc gccgctttag
    47881 ccgcaagaat gaagatatcg agcacagcca tactgtcgat catcattgat acaacatgta
    47941 atcactaagg ccgcgtaagc ggcctttttt atgcataacc tacgaacatt aaggagtaat
    48001 tgaaccacca actcaggatc tcatacgaaa accagtatta accacgtata aaattcataa
    48061 aaaatactga ttgttagtta atttatatta agtagcgcta atagatttaa taatccataa
    48121 tcatttagag gctattctta attatttgcg gtaattcttt attcattcct cggttattac
    48181 gtcatattca gagcaatcct ggtattagtg tcaccaattt catctggcga taatcctgaa
    48241 atgttatgaa cagttcgagc aaactgcttt tacctgctgc gggttagtgc tagtatgaaa
    48301 aagtgagtcc tgtcccgatt ccttcctaat tgtaatgttt cgtaataatg cgatgaaaac
    48361 ctgcaaagag tggcttatag ttaagctaac aaacgagagg gcaagtctag gtcagtaagt
    48421 tttttccatc ccgaaaggtg tccgttagtt caaccactaa gaaggggacg cgttatggat
    48481 gaatactcac ccaaaagaca tgatatcgca cagcttaagt ttctctgtga aaccctgtat
    48541 catgactgtc ttgcaaacct tgaagaaagc aatcatggtt gggttaacga cccaacctcg
    48601 gcgatcaacc tccagttgaa tgaactgatt gagcatattg cgaccttcgc acttaattac
    48661 aaaattaagt ataatgaaga caataagctc attgagcaga tcgacgaata tctggatgac
    48721 acctttatgt tgttcagcag ttatggtatt aatatgcagg ctcttcagaa atggcggaag
    48781 tcaggtaatc gactattccg ttgttttgtc aacgcgacga aagagaatcc tgcgagttta
    48841 tcttgttaga attattacaa ccatgggtag aagtatgtcc gaaaaacctt taacaaaaac
    48901 cgattattta atgcgtttac gtcgttgcca gacaattgac acgctggagc gtgttatcga
    48961 gaaaaataaa tacgaattat cagataatga actggcggta ttttactcag ccgcagatca
    49021 ccgcctcgcc gaattgacca tgaataaact gtacgacaag atcccttcct cagtatggaa
    49081 atttattcgc taataaataa ttcgctttcg gtgctataac cggctgttta ttaagaattt
    49141 tatacttttt cgccatgaag acatacccta tgtgatcttt atcacacaga tgtaatgaga
    49201 acgttctctt cactgacttt tcgtcttact gtgttgccgc attttcagca accggaggtc
    49261 agtaatgagc acagaaaaag aaaagatgat tgctggtgag ttgtatcgct cggcagatga
    49321 gacgttatct cgcgatcgcc tgcgcgctcg tcagcttatt caccgataca atcattccct
    49381 ggcggaagag cacacattac gccagtaaat tctcgctaat ttattcggtc aggtgacaga
    49441 ggcttatatt gagccaacgt ttcgctgtga ttatggctat aacatttttc tcggcaataa
    49501 ttttttcgcc aacttcgatt gcgtgatgct tgatgtctgc cctattcgca tcggtgataa
    49561 ctgtatgttg gcaccaggcg ttcatatcta cacggcaaca catcccatcg accctgtagc
    49621 acgtaatagc ggtgctgaac tggggaaacc cgtcaccatc ggtaataacg tctggattgg
    49681 cggacgcgcg gtcattaacc cttgtgtgac cattggtgat aacgtcgtgg tagcttcagg
    49741 tgcagttgtc acaaaaggtg tcccggacaa cgttgtcgtg ggcggtaatc cagccagaat
    49801 aattaaaaaa ttgtaatcgg tttttcgcaa ctgttataca aaattgtggt agatctgtta
    49861 cttcccctct actattccca cgttaaaata gggtgttccc tggaaagttg cagataccac
    49921 gaaggcaaac gatgaccgaa atacaacgcc tgctgaccga aacgattgag tctctgaata
    49981 cccgcgaaaa acgcgacaac aaaccccgct ttagtatcag ttttatccgt aaacatccgg
    50041 ggctgtttat cggtatgtac gttgcttttt ttgccaccct ggcggtgatg ttgcagtccg
    50101 aaacgctgtc aggctctgtc tggctactgg ttgtattatt tatcctgctt aatggtttct
    50161 tctttttcga tgtctaccca cgctaccgct atgaagatat cgacgtgctg gatttccgcg
    50221 tttgctataa cggcgaatgg tacaacacgc gctttgtacc tgccgcgctg gttgaagcca
    50281 tcttgaactc tccgcgtgtc gcggatgttc ataaggaaca actgcaaaaa atgatcgtcc
    50341 gtaaaggtga actgtctttt tacgatattt ttaccctcgc tcgcgccgaa tcaacatctt
    50401 aagttagggt tacataccag gcgtaaagct ctgcgcctgg tcaaatgaca atgatcgttt
    50461 ccacccatca cttcatgaaa taccagctct acctccttat ctcctgccag cctttttcca
    50521 caatcagata tactttccct acactgtgtt aataaggata tgctggtgag aacacgacat
    50581 ctggtcggcc ttatttcggg agtactgatt ctttcagtat tgctgcctgt cggcttaagc
    50641 atctggctgg cccatcagca ggtagaaaca tcgtttattg aagagctgga tacctattcc
    50701 tcccgcgtcg ctattcgagc caataaggtg gcgacacaag ggaaagatgc gctgcaggag
    50761 ctggaaagat ggcaaggcgc tgcctgtagc gaagcccatc tcatggaaat gcgtcgggta
    50821 tcttacagtt atcgctatat tcaggaagtg gtttatatcg ataacaacgt tccccagtgt
    50881 tcgtctctgg agcatgaaag tccgcccgat acctttcccg agctaggtaa aatttcgaaa
    50941 gatggttatc gtgtctggtt aacatcgcat aacgatttag gcattatccg ttacatggtc
    51001 gccatgggaa cggcacatta tgtcgtcatg atcgaccccg cttcctttat tgatgtcatt
    51061 ccctatagtt catggcaaat tgatgccgcc attattggca atgcccataa cgttgtcata
    51121 accagcagcg atgaaattgc tcagggaatt attaccaggc tacaaaaaac acccggtgag
    51181 catatcgaaa ataatgggat catttacgat atactgccct tcctggagat gaatatttcg
    51241 atcatcacct gggcttcaac gaaaatgttg cagaaaggct ggcatcggca agtctttatt
    51301 tggttaccgc tcgggttggt gattggcctg ctggcagcga tgtttgtgct gcgtattttg
    51361 cgccgtatcc aatcaccgca tcatcggctg caggatgcta tcgaaaatcg tgatatttgc
    51421 gtgcactatc agccgattgt ctccttagcc aatggcaaaa ttgtcggtgc tgaggcactg
    51481 gcgcgctggc cgcagacaga cggtagttgg ttgtcaccag atagttttat tccgctggca
    51541 cagcaaacgg gcctttctga gccattgacg ctactgatta taagaagcgt ctttgaagat
    51601 atgggcgact gcttgcgtca gcatccacag cagcatattt cgatcaatct tgaatccacc
    51661 gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa tgatcaatca ctatcaggtt
    51721 aatcccagac agatcgcgct tgaactcact gaacgcgagt ttgccgatcc gaaaaccagc
    51781 gccccgataa tttctcgcta ccgggaggcg ggccatgaaa tttatcttga tgattttggt
    51841 acggggtatt caagtttaag ttatttacag gatctggatg tcgacattct gaagatcgat
    51901 aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc cgcatatcat cgaaatggca
    51961 aaaacactga aactgaaaat ggtagcggag ggaatcgaaa ccagtaaaca agaagagtgg
    52021 ctacgccagc atggcgtgca ctacggtcag ggctggctct acagcaaggc attaccgaaa
    52081 gaagatttct tacgctgggc cgagcaacat ttgtgaattt ttgggctgac ggcacgccgc
    52141 acagcccgtc acgctaccta ttcgtcgatg attgactcga atccgccata gatcattcgc
    52201 ttgccgtcaa acggcatgga ctcgccgaac tctttcatcc gtgggtccga catcatcttt
    52261 tgattagctg cgtcgcggac ctcttttgaa gggtattcaa tccagctaaa gacaacctct
    52321 tcattctctt ccgctttcac cgccatacga aaatcggtca ctttgccatc cggcacatcg
    52381 ctggcccagc attcgacaat acgaagcgcg ccaaactctt taaacaatgg cgcggcctta
    52441 gcggccattt cccgataggc atccttttta tcggcaggaa cggcaaccac aaaaccatca
    52501 acatacttca tcagacatac ctccgaagcg tactgcgcag ccagacaatt cttgcgacgt
    52561 cagttgtggt taaagtttag ccatgctggg attttttgac gaaacggtgg gaggcagaaa
    52621 tgggtggggg ccctgccagc tacatcccgg cacacgcgtc atctgccttg gctgcttcct
    52681 tccggacctg acctggtaaa cagagtagca ttgcgggaga accaacagag cccccattga
    52741 gagcgttgaa aaccaacgcg caggcgcatt atcactgctg ccccggctaa ttgcaaccca
    52801 ttgcgtgcca gatgctggtt tcttgcgcaa tgcgacttca ctcgggcgtt tttccagatt
    52861 atgctgaaaa attacctcag gaagaaaaga tggaaaaaga agattcattt ccccaacgcg
    52921 tctggcaaat cgtcgccgct attcccgaag gctatgtcac cacttacggt gatgtggcga
    52981 aactggcggg atcgccccgc gccgcgcgcc aggtgggcgg tgtgttaaag cgtctccctg
    53041 aaggcagcac cttaccctgg caccgggtgg ttaatcgcca tggcacaatt tcgctaaccg
    53101 ggccggattt acagcgtcag cgacaggcat tactggcaga aggtgtgatg gtatcgggaa
    53161 gcgggcaaat cgacttgcag cgttatcgct ggaactacta accctctccg g
//
LOCUS       DAJTUM010000017        52605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTUM010000017 DAJTUM010000000
VERSION     DAJTUM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..354
                     /locus_tag="OO471_001094"
     CDS             <1..354
                     /locus_tag="OO471_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6242444.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO471_001095"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO471_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6242445.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO471_001096"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO471_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6242446.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO471_001097"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO471_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6242447.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO471_001098"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO471_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6242448.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO471_001099"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO471_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6242449.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO471_001100"
     CDS             6877..7281
                     /locus_tag="OO471_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6242450.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO471_001101"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO471_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6242451.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO471_001102"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO471_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6242452.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO471_001103"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO471_001103"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6242453.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO471_001104"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO471_001104"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6242454.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO471_001105"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO471_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO471_001106"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO471_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6242455.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO471_001107"
     CDS             complement(13133..13429)
                     /locus_tag="OO471_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6242456.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO471_001108"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO471_001108"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6242457.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPTDFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO471_001109"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO471_001109"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6242458.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO471_001110"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO471_001110"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6242459.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO471_001111"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO471_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6242460.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO471_001112"
     CDS             complement(18686..19372)
                     /locus_tag="OO471_001112"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6242461.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO471_001113"
     CDS             complement(19772..19912)
                     /locus_tag="OO471_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242462.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO471_001114"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO471_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6242463.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO471_001115"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO471_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6242464.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO471_001116"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO471_001116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6242465.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO471_001117"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO471_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6242466.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO471_001118"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO471_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6242467.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO471_001119"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO471_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6242468.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO471_001120"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO471_001120"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6242469.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO471_001121"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO471_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO471_001122"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO471_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6242470.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO471_001123"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO471_001123"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6242471.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO471_001124"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO471_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6242472.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO471_001125"
     CDS             complement(31511..31678)
                     /locus_tag="OO471_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242473.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO471_001126"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO471_001126"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6242474.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO471_001127"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO471_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6242475.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO471_001128"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO471_001128"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6242476.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO471_001129"
     CDS             35470..36114
                     /locus_tag="OO471_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6242477.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO471_001130"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO471_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO471_001131"
     CDS             37189..37452
                     /locus_tag="OO471_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6242478.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO471_001132"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO471_001132"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6242479.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO471_001133"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO471_001133"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6242480.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO471_001134"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO471_001134"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6242481.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO471_001135"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO471_001135"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6242482.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO471_001136"
     CDS             42150..43691
                     /locus_tag="OO471_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6242483.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO471_001137"
     CDS             43663..44526
                     /locus_tag="OO471_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6242484.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO471_001138"
     CDS             complement(44740..45522)
                     /locus_tag="OO471_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6242485.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO471_001139"
     CDS             complement(45519..46592)
                     /locus_tag="OO471_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6242486.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO471_001140"
     CDS             complement(46714..46875)
                     /locus_tag="OO471_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6242487.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO471_001141"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO471_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6242488.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO471_001142"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO471_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6242489.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO471_001143"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO471_001143"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6242490.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO471_001144"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO471_001144"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6242491.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO471_001145"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO471_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6242492.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO471_001146"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO471_001146"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6242493.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12530 a        14234 c        13182 g        12659 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatatctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggtggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcaatgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatgttttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagctt gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatggtat gcgaa
//
LOCUS       DAJTUM010000018        49830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTUM010000018 DAJTUM010000000
VERSION     DAJTUM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..1224
                     /locus_tag="OO471_001147"
     CDS             <1..1224
                     /locus_tag="OO471_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6242494.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO471_001148"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO471_001148"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6242495.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO471_001149"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO471_001149"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6242496.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO471_001150"
     CDS             2714..3427
                     /locus_tag="OO471_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6242497.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO471_001151"
     CDS             complement(3399..4076)
                     /locus_tag="OO471_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6242498.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO471_001152"
     CDS             complement(4070..4747)
                     /locus_tag="OO471_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6242499.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO471_001153"
     CDS             complement(4735..5118)
                     /locus_tag="OO471_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6242500.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO471_001154"
     CDS             complement(5134..5442)
                     /locus_tag="OO471_001154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242501.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO471_001155"
     CDS             complement(5443..6021)
                     /locus_tag="OO471_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6242502.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO471_001156"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="OO471_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO471_001157"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO471_001157"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6242503.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO471_001158"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO471_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6242504.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO471_001159"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO471_001159"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6242505.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO471_001160"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO471_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6242506.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO471_001161"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO471_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6242507.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO471_001162"
     CDS             12291..13031
                     /locus_tag="OO471_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6242508.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO471_001163"
     CDS             13198..14148
                     /locus_tag="OO471_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6242509.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO471_001164"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO471_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO471_001165"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO471_001165"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6242510.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO471_001166"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO471_001166"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6242511.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO471_001167"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO471_001167"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6242512.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO471_001168"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO471_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6242513.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO471_001169"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO471_001169"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6242514.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO471_001170"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO471_001170"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6242515.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO471_001171"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO471_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6242516.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO471_001172"
     CDS             23768..23827
                     /locus_tag="OO471_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6242517.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="OO471_001173"
     CDS             complement(23928..24530)
                     /locus_tag="OO471_001173"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6242518.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="OO471_001174"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO471_001174"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO471_001175"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO471_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6242519.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="OO471_001176"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="OO471_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6242520.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="OO471_001177"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="OO471_001177"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6242521.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="OO471_001178"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="OO471_001178"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6242522.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="OO471_001179"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="OO471_001179"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6242523.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="OO471_001180"
     CDS             complement(29676..29759)
                     /locus_tag="OO471_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242524.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="OO471_001181"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="OO471_001181"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6242525.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="OO471_001182"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="OO471_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6242526.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="OO471_001183"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="OO471_001183"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6242527.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="OO471_001184"
     CDS             34816..35559
                     /locus_tag="OO471_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6242528.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO471_001185"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO471_001185"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6242529.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="OO471_001186"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="OO471_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="OO471_001187"
     CDS             37568..38227
                     /locus_tag="OO471_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6242530.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO471_001188"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO471_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6242531.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="OO471_001189"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="OO471_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6242532.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="OO471_001190"
     CDS             39893..40300
                     /locus_tag="OO471_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6242533.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO471_001191"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO471_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6242534.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="OO471_001192"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="OO471_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6242535.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="OO471_001193"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="OO471_001193"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6242536.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="OO471_001194"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="OO471_001194"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6242537.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="OO471_001195"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="OO471_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6242538.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="OO471_001196"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="OO471_001196"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6242539.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO471_001197"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO471_001197"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6242540.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="OO471_001198"
     CDS             47705..47776
                     /locus_tag="OO471_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242541.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="OO471_001199"
     CDS             complement(47773..47898)
                     /locus_tag="OO471_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242542.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO471_001200"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO471_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6242543.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12452 c        13407 g        11920 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgcgaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DAJTUM010000019        49529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTUM010000019 DAJTUM010000000
VERSION     DAJTUM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO471_001201"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO471_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6242544.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPTMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO471_001202"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO471_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6242545.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO471_001203"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO471_001203"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6242546.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO471_001204"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO471_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6242547.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO471_001205"
     CDS             5718..6659
                     /locus_tag="OO471_001205"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6242548.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO471_001206"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO471_001206"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6242549.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO471_001207"
     CDS             7679..8929
                     /locus_tag="OO471_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6242550.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO471_001208"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO471_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO471_001209"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO471_001209"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6242551.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO471_001210"
     CDS             10866..12116
                     /locus_tag="OO471_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6242552.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO471_001211"
     CDS             complement(12172..13260)
                     /locus_tag="OO471_001211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6242553.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO471_001212"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO471_001212"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6242554.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO471_001213"
     CDS             complement(14194..15243)
                     /locus_tag="OO471_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6242555.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO471_001214"
     CDS             15342..16223
                     /locus_tag="OO471_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6242556.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO471_001215"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO471_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6242557.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO471_001216"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO471_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO471_001217"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO471_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OO471_001218"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OO471_001218"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6242558.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OO471_001219"
     CDS             21991..23067
                     /locus_tag="OO471_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6242559.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OO471_001220"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OO471_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6242560.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OO471_001221"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OO471_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6242561.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OO471_001222"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OO471_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6242562.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OO471_001223"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OO471_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6242563.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO471_001224"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO471_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO471_001225"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO471_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6242564.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OO471_001226"
     CDS             complement(30809..31048)
                     /locus_tag="OO471_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6242565.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO471_001227"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO471_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6242566.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO471_001228"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO471_001228"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6242567.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="OO471_001229"
     CDS             complement(32934..33629)
                     /locus_tag="OO471_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6242568.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="OO471_001230"
     CDS             complement(33626..34024)
                     /locus_tag="OO471_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6242569.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="OO471_001231"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="OO471_001231"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6242570.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO471_001232"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO471_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6242571.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO471_001233"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO471_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37377..38138
                     /locus_tag="OO471_001234"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="OO471_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="OO471_001235"
     CDS             complement(38255..38833)
                     /locus_tag="OO471_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6242572.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="OO471_001236"
     CDS             39003..39590
                     /locus_tag="OO471_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6242573.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="OO471_001237"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="OO471_001237"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6242574.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO471_001238"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO471_001238"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6242575.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="OO471_001239"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="OO471_001239"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6242576.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="OO471_001240"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="OO471_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6242577.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="OO471_001241"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="OO471_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6242578.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="OO471_001242"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="OO471_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6242579.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="OO471_001243"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="OO471_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6242580.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="OO471_001244"
     CDS             complement(48911..49018)
                     /locus_tag="OO471_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6242581.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49529)
                     /locus_tag="OO471_001245"
                     /pseudo
     CDS             complement(49078..49529)
                     /locus_tag="OO471_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12202 c        13311 g        12061 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgt cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgtttct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaaaatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca gggaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattg
//
LOCUS       DAJTUM010000020        48323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTUM010000020 DAJTUM010000000
VERSION     DAJTUM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            177..470
                     /locus_tag="OO471_001246"
     CDS             177..470
                     /locus_tag="OO471_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6242582.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO471_001247"
     CDS             complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO471_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6242583.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1912..2364)
                     /locus_tag="OO471_001248"
     CDS             complement(1912..2364)
                     /locus_tag="OO471_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6242584.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO471_001249"
                     /gene_synonym="yicO"
     CDS             complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO471_001249"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6242585.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3926..5692
                     /gene="adeD"
                     /locus_tag="OO471_001250"
     CDS             3926..5692
                     /gene="adeD"
                     /locus_tag="OO471_001250"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6242586.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO471_001251"
     CDS             complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO471_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6242587.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO471_001252"
     CDS             complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO471_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6242588.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO471_001253"
     CDS             complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO471_001253"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6242589.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO471_001254"
     CDS             complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO471_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6242590.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO471_001255"
     CDS             complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO471_001255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6242591.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO471_001256"
     CDS             complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO471_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6242592.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO471_001257"
     CDS             complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO471_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6242593.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13514..13603
                     /gene="tisB"
                     /locus_tag="OO471_001258"
     CDS             13514..13603
                     /gene="tisB"
                     /locus_tag="OO471_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6242594.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13883..15067
                     /gene="emrD"
                     /locus_tag="OO471_001259"
     CDS             13883..15067
                     /gene="emrD"
                     /locus_tag="OO471_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6242595.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15075..15572)
                     /locus_tag="OO471_001260"
     CDS             complement(15075..15572)
                     /locus_tag="OO471_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6242596.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15569..15931)
                     /locus_tag="OO471_001261"
     CDS             complement(15569..15931)
                     /locus_tag="OO471_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6242597.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15921..16268)
                     /locus_tag="OO471_001262"
     CDS             complement(15921..16268)
                     /locus_tag="OO471_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6242598.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16377..16790
                     /locus_tag="OO471_001263"
     CDS             16377..16790
                     /locus_tag="OO471_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242599.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16837..18279)
                     /locus_tag="OO471_001264"
                     /pseudo
     CDS             complement(16837..18279)
                     /locus_tag="OO471_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18314..20029)
                     /locus_tag="OO471_001265"
     CDS             complement(18314..20029)
                     /locus_tag="OO471_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6242600.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVIITFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20196..21089
                     /locus_tag="OO471_001266"
     CDS             20196..21089
                     /locus_tag="OO471_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6242601.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21086..21900)
                     /locus_tag="OO471_001267"
                     /pseudo
     CDS             complement(21086..21900)
                     /locus_tag="OO471_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21900..23516)
                     /locus_tag="OO471_001268"
     CDS             complement(21900..23516)
                     /locus_tag="OO471_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6242602.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23811..24485
                     /locus_tag="OO471_001269"
     CDS             23811..24485
                     /locus_tag="OO471_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6242603.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24524..26185)
                     /locus_tag="OO471_001270"
     CDS             complement(24524..26185)
                     /locus_tag="OO471_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6242604.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26381..26809)
                     /gene="ibpB"
                     /locus_tag="OO471_001271"
     CDS             complement(26381..26809)
                     /gene="ibpB"
                     /locus_tag="OO471_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6242605.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26921..27334)
                     /gene="ibpA"
                     /locus_tag="OO471_001272"
     CDS             complement(26921..27334)
                     /gene="ibpA"
                     /locus_tag="OO471_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6242606.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27328..27400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27639..27971
                     /locus_tag="OO471_001273"
     CDS             27639..27971
                     /locus_tag="OO471_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6242607.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27973..29217)
                     /locus_tag="OO471_001274"
     CDS             complement(27973..29217)
                     /locus_tag="OO471_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6242608.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTWTPDSQWLVFDVRPSG
                     ASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYD
                     FHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQ
                     LDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEE
                     GWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRG
                     VVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPR
                     KLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVF
                     SPDGQWLAWMEGGQLWITETDR"
     gene            29318..30418
                     /gene="cbrA"
                     /locus_tag="OO471_001275"
     CDS             29318..30418
                     /gene="cbrA"
                     /locus_tag="OO471_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6242609.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30379..31716)
                     /locus_tag="OO471_001276"
     CDS             complement(30379..31716)
                     /locus_tag="OO471_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6242610.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31791..32939)
                     /gene="dgoD"
                     /locus_tag="OO471_001277"
                     /pseudo
     CDS             complement(31791..32939)
                     /gene="dgoD"
                     /locus_tag="OO471_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32936..33553)
                     /gene="dgoA"
                     /locus_tag="OO471_001278"
     CDS             complement(32936..33553)
                     /gene="dgoA"
                     /locus_tag="OO471_001278"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6242611.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33537..34415)
                     /gene="dgoK"
                     /locus_tag="OO471_001279"
     CDS             complement(33537..34415)
                     /gene="dgoK"
                     /locus_tag="OO471_001279"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6242612.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34412..35101)
                     /gene="dgoR"
                     /locus_tag="OO471_001280"
     CDS             complement(34412..35101)
                     /gene="dgoR"
                     /locus_tag="OO471_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6242613.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35379..36035
                     /locus_tag="OO471_001281"
     CDS             35379..36035
                     /locus_tag="OO471_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242614.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36081..36893)
                     /gene="yidA"
                     /locus_tag="OO471_001282"
     CDS             complement(36081..36893)
                     /gene="yidA"
                     /locus_tag="OO471_001282"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6242615.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37007..37405)
                     /locus_tag="OO471_001283"
     CDS             complement(37007..37405)
                     /locus_tag="OO471_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6242616.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37645..40059)
                     /gene="gyrB"
                     /locus_tag="OO471_001284"
     CDS             complement(37645..40059)
                     /gene="gyrB"
                     /locus_tag="OO471_001284"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6242617.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40088..41161)
                     /gene="recF"
                     /locus_tag="OO471_001285"
     CDS             complement(40088..41161)
                     /gene="recF"
                     /locus_tag="OO471_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6242618.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41161..42261)
                     /gene="dnaN"
                     /locus_tag="OO471_001286"
     CDS             complement(41161..42261)
                     /gene="dnaN"
                     /locus_tag="OO471_001286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6242619.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42266..43669)
                     /gene="dnaA"
                     /locus_tag="OO471_001287"
     CDS             complement(42266..43669)
                     /gene="dnaA"
                     /locus_tag="OO471_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6242620.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43966..44046
                     /locus_tag="OO471_001288"
     CDS             43966..44046
                     /locus_tag="OO471_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242621.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44276..44416
                     /gene="rpmH"
                     /locus_tag="OO471_001289"
     CDS             44276..44416
                     /gene="rpmH"
                     /locus_tag="OO471_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6242622.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44433..44792
                     /gene="rnpA"
                     /locus_tag="OO471_001290"
     CDS             44433..44792
                     /gene="rnpA"
                     /locus_tag="OO471_001290"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6242623.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44756..45013
                     /gene="yidD"
                     /locus_tag="OO471_001291"
     CDS             44756..45013
                     /gene="yidD"
                     /locus_tag="OO471_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6242624.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45016..46662
                     /gene="yidC"
                     /locus_tag="OO471_001292"
     CDS             45016..46662
                     /gene="yidC"
                     /locus_tag="OO471_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6242625.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46768..48132
                     /gene="mnmE"
                     /locus_tag="OO471_001293"
     CDS             46768..48132
                     /gene="mnmE"
                     /locus_tag="OO471_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6242626.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11985 a        13061 c        12217 g        11060 t
ORIGIN      
        1 cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata
       61 taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga
      121 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
      181 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
      241 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
      301 gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
      361 cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc
      421 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
      481 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
      541 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
      601 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
      661 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
      721 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
      781 aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg
      841 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc
      901 aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc
      961 cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga
     1021 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
     1081 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     1141 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     1201 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     1261 cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat
     1321 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     1381 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     1441 aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc
     1501 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     1561 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     1621 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     1681 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     1741 ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg tgttgcttga
     1801 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     1861 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     1921 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     1981 atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga cctgagcatc
     2041 ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca gcaggttctg
     2101 ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg cgttttgcag
     2161 gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat
     2221 ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg
     2281 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     2341 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     2401 aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga gctgcgacca
     2461 ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg
     2521 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     2581 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     2641 tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga accatcgcca
     2701 ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca gccgttagcc
     2761 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     2821 taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     2881 tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca attaatgttc
     2941 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     3001 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     3061 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     3121 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     3181 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     3241 tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat ccgattccac
     3301 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     3361 acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
     3421 aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
     3481 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     3541 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     3601 ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     3661 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     3721 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     3781 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     3841 acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     3901 ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
     3961 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     4021 tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt ccggcccaat
     4081 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata ctccggcttt
     4141 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     4201 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     4261 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     4321 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     4381 cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     4441 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     4501 aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc tgacgctgga
     4561 cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg cgggtattga
     4621 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     4681 gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac cgttgatcaa
     4741 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     4801 ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     4861 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
     4921 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     4981 cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct tacaggcgga
     5041 agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg cccgccagcc
     5101 agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc gggtcattga
     5161 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     5221 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     5281 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg cgacagtcag
     5341 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     5401 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag
     5461 tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
     5521 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     5581 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     5641 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaaa
     5701 gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     5761 tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc atcaggcaga
     5821 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga
     5881 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     5941 ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     6001 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     6061 gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc gtggcgataa
     6121 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     6181 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     6241 ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac
     6301 caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
     6361 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
     6421 cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg
     6481 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
     6541 agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac
     6601 ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc
     6661 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     6721 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     6781 ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg aacggcagga
     6841 attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc acgccataag
     6901 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
     6961 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
     7021 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     7081 gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg ggttactcct
     7141 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
     7201 agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
     7261 gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
     7321 aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
     7381 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
     7441 cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac caattaacat
     7501 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
     7561 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa
     7621 attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc
     7681 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
     7741 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata
     7801 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt
     7861 gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc
     7921 gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca
     7981 gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca
     8041 gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg
     8101 cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa
     8161 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
     8221 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat
     8281 aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa
     8341 cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
     8401 gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc gcgtgaagta
     8461 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
     8521 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
     8581 caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg
     8641 cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
     8701 gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag
     8761 catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt
     8821 cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
     8881 taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc
     8941 catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg
     9001 acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat
     9061 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa caatgcccgc
     9121 ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac gcgccacgtc
     9181 gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag
     9241 ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc ccagtaacaa
     9301 ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat cgcgccaggt
     9361 ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccagcc
     9421 atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa agcgcgacag
     9481 ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca agtaccagac
     9541 cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga gcggcagcca
     9601 ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg tcagaccgcc
     9661 agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
     9721 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
     9781 ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac taccgatcat
     9841 caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
     9901 cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag
     9961 accaaacgga aataacagca ccgccatatc agggcgctca accagatgca ggctgatact
    10021 ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
    10081 ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc tacgtcgtta
    10141 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    10201 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg
    10261 cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc
    10321 gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc atccgggcta
    10381 cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga
    10441 ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct tagcagctcc
    10501 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    10561 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc
    10621 agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    10681 gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg
    10741 atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg
    10801 gatcggactg attacgctgc actttcacga catcttccag cttagcgatt tggcttatca
    10861 tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct
    10921 gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa
    10981 cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg ttgttatgag
    11041 ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca
    11101 tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat
    11161 tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga
    11221 ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg gcggcaaaaa
    11281 cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca
    11341 tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca
    11401 tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg
    11461 cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact
    11521 ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg
    11581 tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc
    11641 ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca
    11701 actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt
    11761 catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat
    11821 cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc
    11881 ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa atatagttgg
    11941 tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcagca
    12001 tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca
    12061 cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt ttggcagcgt
    12121 taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca acggcttttt
    12181 ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt
    12241 ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct
    12301 gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc
    12361 cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccag
    12421 tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc accaggttag
    12481 tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccattc
    12541 cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg atttgcgtgc
    12601 tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct
    12661 taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg
    12721 tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg
    12781 aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt
    12841 gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg
    12901 cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg
    12961 ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa
    13021 tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg cagataacaa
    13081 aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact
    13141 gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa
    13201 ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg tccaaatatt
    13261 actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt tatgcggggg
    13321 ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta
    13381 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
    13441 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
    13501 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
    13561 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
    13621 ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagtcggatg
    13681 cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat tgccgctttt
    13741 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
    13801 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
    13861 ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt
    13921 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
    13981 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
    14041 gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg tcggtcgccg
    14101 accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
    14161 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
    14221 cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt tgcgccatgc
    14281 taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg
    14341 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
    14401 tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg atgcaccgcg
    14461 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
    14521 gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct ccggcgtgct
    14581 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
    14641 tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct tctcaacgtt
    14701 gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga tccccgactg
    14761 gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc
    14821 cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct tcctggcggg
    14881 cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
    14941 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga ccttaatggg
    15001 attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc
    15061 cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
    15121 atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc
    15181 gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg
    15241 cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg
    15301 gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg
    15361 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
    15421 tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg
    15481 tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc
    15541 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
    15601 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
    15661 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
    15721 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
    15781 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
    15841 agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg
    15901 caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccgcc
    15961 acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc
    16021 agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg
    16081 gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc
    16141 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc
    16201 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa
    16261 attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa
    16321 gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg
    16381 gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa
    16441 tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa
    16501 gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg
    16561 aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa
    16621 gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg
    16681 aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt
    16741 tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa
    16801 taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc
    16861 caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc
    16921 atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg
    16981 acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    17041 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    17101 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag
    17161 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    17221 aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt
    17281 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    17341 atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    17401 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    17461 gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg
    17521 cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata
    17581 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    17641 gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta
    17701 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    17761 atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac
    17821 cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa
    17881 atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    17941 aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt
    18001 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    18061 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    18121 cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac
    18181 cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    18241 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    18301 caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    18361 gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac
    18421 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc
    18481 caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt
    18541 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    18601 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    18661 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    18721 ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg
    18781 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttcg
    18841 cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    18901 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    18961 tgaataatgt actggcgcta ttcagaaagc cgttgaaggt aatgatcacc gcaccaaata
    19021 acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc agcgtcgggt
    19081 aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca
    19141 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    19201 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    19261 aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    19321 caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa
    19381 agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    19441 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    19501 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    19561 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    19621 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    19681 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    19741 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    19801 ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg
    19861 aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    19921 gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg
    19981 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    20041 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    20101 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    20161 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    20221 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    20281 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    20341 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    20401 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta
    20461 ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    20521 agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt cagcaaagcg
    20581 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    20641 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    20701 ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    20761 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    20821 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    20881 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    20941 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    21001 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    21061 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    21121 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    21181 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    21241 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    21301 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    21361 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    21421 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta
    21481 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    21541 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    21601 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    21661 aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    21721 cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc
    21781 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    21841 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    21901 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    21961 agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    22021 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    22081 tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc
    22141 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg
    22201 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    22261 tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac
    22321 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    22381 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    22441 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    22501 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc
    22561 atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg
    22621 gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc
    22681 agggcaaaac cgccttccag gaacaacgat ttcagtggct cggcgctcag actgaactct
    22741 tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata
    22801 aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg
    22861 taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc
    22921 atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat caccaggaag
    22981 gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta
    23041 tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg
    23101 ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc caccgcgtcc
    23161 tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag
    23221 ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca
    23281 aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg
    23341 ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc
    23401 ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc ggcgaaggga
    23461 aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact gagcatttcc
    23521 acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg cagacctgga
    23581 gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc gtgatcgctt
    23641 tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga catctgtgag
    23701 cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat cgattaagca
    23761 gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag atgatctaca
    23821 aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg ctaaacagcc
    23881 ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg actatccgta
    23941 aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc agcggcactt
    24001 acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg gtggaggtgt
    24061 taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc
    24121 ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc
    24181 gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg ccggtaaaac
    24241 tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag
    24301 agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc gccgatagac
    24361 tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt
    24421 catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat
    24481 actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg atactccaga
    24541 agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag
    24601 tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca aacgccagcg
    24661 ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg
    24721 ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat
    24781 aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt
    24841 tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac
    24901 ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca
    24961 taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct ggcacaaaga
    25021 cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa
    25081 cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg
    25141 gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca
    25201 gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca
    25261 ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca
    25321 aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc agttgcgcat
    25381 tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg ataatggtat
    25441 ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata attttgtcgc
    25501 cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa
    25561 tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt
    25621 ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc
    25681 aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg
    25741 gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta ttggttaccg
    25801 caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg
    25861 cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg
    25921 agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca
    25981 ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct
    26041 gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta
    26101 tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac
    26161 tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta
    26221 cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag
    26281 aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa
    26341 aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac
    26401 gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa
    26461 tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca gccagcgtaa
    26521 aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg
    26581 gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc tctaaatctt
    26641 cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa
    26701 tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg gccagtttgt
    26761 caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc
    26821 cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg
    26881 agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt tcgatacggc
    26941 gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca
    27001 ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt
    27061 cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca
    27121 ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag
    27181 cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc
    27241 cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag
    27301 aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag
    27361 aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct
    27421 catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat cagcggactt
    27481 ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc
    27541 gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact
    27601 catcaggcca acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag
    27661 cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata
    27721 ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg attggcgacg
    27781 atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtaa
    27841 tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcgcgcg
    27901 ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc
    27961 ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc
    28021 cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt
    28081 ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc gattatccag
    28141 cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt
    28201 gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca gttgcacaat
    28261 gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca
    28321 gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg ttaaacgtcg
    28381 ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta acggcgcatc
    28441 acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata gctccggcac
    28501 tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca
    28561 tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt
    28621 actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct tttgtacgtt
    28681 gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg gatcgagttg
    28741 atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc taaagacatg
    28801 cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc
    28861 gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga agtcgtaatg
    28921 ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg acttcggatg
    28981 aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc
    29041 tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac
    29101 gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat tggtattggt
    29161 attggtattg gtattggtga gcaggtgatt acggggagca aaggttatct gtttcatggc
    29221 gcggtcctga tacaaaacgg ttcccgccat catacttcac aaggacttca ctttcagaca
    29281 atagtctttt gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat
    29341 tattggcctc ggcccggcag ggtcggcgtt ggcacgaaag ttagccggca aaatgcaggt
    29401 gatcgcgctg gataaaaagc accagtgcgg tactgaaggt ttcagcaaac cttgcggcgg
    29461 tctgctggca ccggacgcgc agcgatcttt tattcgcgat ggactgacgc ttcctgtcga
    29521 tgtgatcgcc aatccacaga ttttcagcgt caaaactgtc gacgtcgccg catcgctcac
    29581 gcgtaactac cagcgaagct atatcaatat taatcgccat gctttcgact tgtggatgaa
    29641 atcgctgatc cccgccagcg ttgaggttta tcacgatagc ctgtgccgaa aaatctggcg
    29701 tgaggatgat aaatggcatg tcatttttcg tgcagacggc tgggagcaac atattaccgc
    29761 ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg cggcgacatc tctacccgga
    29821 tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc gcagagaaac atccggtgcc
    29881 gttctactcc tgcatctttg ataatgcgat aactgactgt tactcatgga gtatcagcaa
    29941 agatggttat tttatctttg gcggtgccta tccaatgaaa gacggtcaga cgcgtttcac
    30001 gacgctgaaa gagaaaatga gcgcctttca gttccagttt ggcaaagcgg tgaaaagtga
    30061 gaaatgcacg gtgttgtttc cctcacgctg gcaggatttt gtctgtggta aggacaacgc
    30121 ctttctgatt ggtgaagcgg cgggatttat cagcgccagc tcgctggagg ggattagcta
    30181 tgcgctggat agcgcagaga ttctgcgtgc ggtgttcctg aaacagccgg agaagagcaa
    30241 cgccgcttac tggcgcgcca cacacaaact gcgtttaaaa ctcttcggca agatagtaaa
    30301 aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg gtgtggcgca
    30361 tattccacag ttgaaagatt atccaacgcg cttcacatca cccaccagca ggatgtaaga
    30421 gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga aaccgtaacc
    30481 ctgcgccagg taccccacca ccagcggaac ggtgatgccg cccagaccac cggcgaagtt
    30541 aaacacgccg ccggttaaac caatcaggcg catcggtgcc agagaagaga ccagcgacca
    30601 ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca taatcatcat
    30661 cggatcgttg gtgtagttag cgcccataat gcaggtggag atcagcaagc cgcagataat
    30721 cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat ccgcgaccca
    30781 gccagagagc aggacgccga caaacgccgc gaggaatggc accgtggtca taaagcctgc
    30841 tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg ttaagaaaaa
    30901 acacagtgca gaagccaccg caaattgccc aagataaacg ccgatcagtt tacgatggaa
    30961 caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca ccggcgcatc
    31021 accatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc tgataccttt
    31081 ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa tgccgatacc
    31141 accagtgaca atgaacaccc agtgccagct caacatctcc tgaatccaga tcagcagcgg
    31201 agtcaaaaac gccagaccga caaactgacc agacgtataa aaaccaacgg cggaagcgcg
    31261 ttcatgttcc gggaaccagc tggtcaccat ccggttattg gtcgggaaag cgggcgcttc
    31321 gaaaataccg gttatcgcac gcagaccaat taatgacatt aatcccgtgg caaagccttg
    31381 gaataaagtc gccactgacc aaccaaatat cgcaataaaa taagtcacgc gagaacctac
    31441 gcgatctaaa aaccaaccgc cggggatctg gcatagcgta taaagccagg cgaaggccga
    31501 aaatacatag cccatttccg ctttcgtaat gccgaactct tcctgaatat gggcggaagc
    31561 cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga tcatcaccag
    31621 cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca tagcgagctt
    31681 tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac agagggttat
    31741 atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa ttaccactct
    31801 gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc atttttactg
    31861 aactcaataa ctttagcttc gtcgatttcc acacctaagc ccggtttcgt taacggttta
    31921 aagaagccgc caaccatgct gaagtcttct ttgtttttca caaagtcgag taactccgcg
    31981 cctttgttgt aatgaatgcc catactttgt tactgaagta ccgcgttata ggaaacaaag
    32041 tcgatatgca ggcaagccgc cagtgcaatc ggtccgaacg gacagtgcgg cgcaagggtc
    32101 acatcatagg cttctgccat tccagcaatt ttgtagcatt cggtaatacc gcctgcatgg
    32161 gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacgcgttt aaaatcgaaa
    32221 cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc caatttcggg
    32281 tagtattcgg cttgttccgc cagcaccggc tcttcaataa acagcgggcg atacggctcc
    32341 agttctttaa tcagcacttt cgccattggc gcgctgacgc gaccgtggaa atcaagacca
    32401 aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac tgccgcatct
    32461 accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt gaaggtatcg
    32521 aagccgattt cgcgcagggt tttaatgccg tcgataacat ccgccggacg atcgccgccg
    32581 acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg ccagactggc
    32641 gcattcagca ctttgccttt gatatcccat aacgcctggt cgatcccggc gatggcgctc
    32701 atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca taagtcgttg
    32761 atgcgcgaag gatcctgacc aatcaaatag tcacccagct cgtgaactgc cgcttccacc
    32821 gtacgggcgc ggccttcaat cacgggctcg ccccaaccga ccacgccttc atcggtttca
    32881 attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt gattttcatt
    32941 gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg
    33001 actgcccggc gcgataaaga tcgctgccta agcccgcccc aacacaacct gcgtctatcc
    33061 actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg tccggtggta
    33121 ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga aatattttca
    33181 gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc
    33241 aaacggtcat gccgtagccc accgcgcggc ggatcacttc actatggata ttgggcgtaa
    33301 cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc agtaccgtac
    33361 ctgcgccaat caacgccttg tcgccatacg catcaacgat ggcgggaatg ctctgctccc
    33421 attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa
    33481 catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc gggagtttag
    33541 tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc gcccgccacc
    33601 gccgccacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt
    33661 ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact ggcaacctct
    33721 gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag tgttcccagc
    33781 acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt aaggccgcgc
    33841 tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc
    33901 aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg
    33961 atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcataac ataaagagag
    34021 gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg
    34081 ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc aacagacgtt
    34141 aattgttcgc caatagacga aaaacgggca ggaacagata aatacggagc aactttccag
    34201 ccgacattgc tgccgaccat tcctgccatc accactggcg tattttcttc acgccagtcg
    34261 gtcgtgactt ctgctaacac tgcagccgga gattttccgt tcaggcgcgt gacgcctgct
    34321 tctgattgcc tgctctccag gcagtggtcg ccctgataaa gccaggcgcg cagattggtc
    34381 gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg
    34441 agctggcaat catggtaagc gccgcctgct ctgccgcatc gccgtcctga tgccgtatcg
    34501 catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca tcgcccatcc
    34561 aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt tgctgtaaca
    34621 ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct tcgttaaacg
    34681 cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc tgcgccagat
    34741 cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc agatttcgca
    34801 cttcgctcat ggcactgata agccgtgggt cgtagtcgtt ttccagcacc cattgcagta
    34861 cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg cgataacgtt
    34921 tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg atgttgcgcg
    34981 aggttgcaaa ctcttcacag agctccgcct cagccggaag tggcgagcca ggtacgtatt
    35041 tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta ttgagagtca
    35101 tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat gcataacgcg
    35161 gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gcgcgcgtaa tgtagtacaa
    35221 acttatatta ttgcgctaca atttagatca aaaaaagaac aacacataaa aaaatgacat
    35281 gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa agaagagtat
    35341 gctttcaacg gcttagctac tcgtttaaag gattaatcat gaagttgaat tttaagggat
    35401 tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt atctcgtatg
    35461 ctctggtttc ccataccgca aagggtagtt caggaaagta tcaatcgcag tcagacacca
    35521 tcactgggct atcgcaggca aaagatagta atggaacaaa aggctatgtt tttgtagggg
    35581 aatcgctgga ttaccttatc actgatggtg ccgatgacat cgttaagatg ctcaatgatc
    35641 cagcacttaa ccggcacaat attcaggttg ccgatgacgc aagatttgtt ttaaatgcgg
    35701 ggaaaaagaa atttaccggc acaatatcgc tttactacta ctggaataac gaagaagaaa
    35761 aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc aggtcactgg
    35821 aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca aaggtgatgg
    35881 cgttctacca tccatttaaa gtgcgatttt atgaatacta ttcacccaga ggcattccgg
    35941 atggtgtttc cgcagcatta ctgccagtga ctgttacact ggacatcatt actgcaccgc
    36001 tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa acgcgtttat
    36061 gctgtttgcc cgcccacaga ttaattcagc acatacttct caatagcaaa cgccacgcca
    36121 tcttcaaggt tggattttgt gacaaagttc gccacttctt tcaccgaagg aatagcgtta
    36181 tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc ctgatcgcca
    36241 atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt cacacccgta
    36301 cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt cagcacggta
    36361 tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat ggcgggttca
    36421 tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc ttcgcagaac
    36481 accagcggaa ttgtggcaac gaaggattca tgcaccgtgt agtagctgat atcgcggtta
    36541 gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc gcgagagagt
    36601 ttttccagga aacgatagtc gtcatagctg agagcggttt gcgccacggt gctaccatca
    36661 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
    36721 tgcagctctt tcaggtagtt gtgcacacct gcatacggac gacccgtcgt tagcacgaca
    36781 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
    36841 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
    36901 caaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
    36961 ccgagggaaa aggggatgca acgcgggttg catgctcccc atttgcttag cggaagagtt
    37021 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt gctgacactt
    37081 caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt aaactggaag
    37141 ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    37201 ccgactccag ttgctcgccg ctaactgatt gatttctctg ttgattactc aaccaggttg
    37261 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccaccct
    37321 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcac
    37381 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa
    37441 aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat
    37501 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttac ccggcctaca
    37561 aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg tagcgcatca
    37621 ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca gggcgttctc
    37681 ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga acaactggtc
    37741 agcggcaatc gcatccttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt
    37801 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    37861 gccacgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    37921 acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca gtttctcacc
    37981 cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca gcggatagtc
    38041 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    38101 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    38161 cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt ccgtcaacgt
    38221 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    38281 cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc cagccaatgc
    38341 cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga tctggtactg
    38401 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    38461 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    38521 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    38581 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag
    38641 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
    38701 gacgttaagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    38761 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
    38821 gcgttcctgg cagtctgcca gtttgcccgg caggccagct aagtcgagcg cacctttacg
    38881 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    38941 gccaaccacg attttcgcat cagttgggtt ttccagcagg tattccgcca gcagttcgtt
    39001 catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    39061 ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt cacgcgcatc
    39121 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcatt
    39181 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    39241 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcagcgccac
    39301 ttcaacgcca ataccgtctt tttcggtgga gaagtagaag atatttgggt ggatcggcgt
    39361 tttgttcttg ttcagatatt caacgaacgc tttgatgccg ccttcatagt ggaagtggtc
    39421 ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    39481 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    39541 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    39601 cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctgaat
    39661 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    39721 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    39781 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    39841 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    39901 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    39961 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    40021 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    40081 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    40141 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    40201 ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    40261 tcaggtagag acaccgccgc ccgctttcac gagtgaggaa ctctccttgc gccagacgta
    40321 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    40381 cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat
    40441 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    40501 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca tccatatcgg
    40561 ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca
    40621 gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc gccgcattgc
    40681 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccaggt tcgttgtgaa
    40741 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    40801 cttctggcgt tatcaactgc atcggcatca ggtgcgccag ttccgcgact ttatgcccgt
    40861 ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    40921 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc tcatggcgaa
    40981 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    41041 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
    41101 aagcgagatc cgcggtttca atattgcgga aatcgcggat caacaagcgg gtgagggaca
    41161 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    41221 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
    41281 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    41341 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtaattt tcagctggtt
    41401 ttcgctgaca tacaggcgca caccgcggaa tttctcgtta gagagaatcg ccgcgcgagc
    41461 aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt tcttcggcag
    41521 aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa taaagtcgcc
    41581 aacgtgggca cgaatgttat tgctgccaat ctgcacacgc agcagattgt cgccgccgtc
    41641 gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat ggcttggcaa
    41701 agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg
    41761 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa catcctgatg
    41821 cgccatcgaa aactgggttg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    41881 ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
    41941 cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct gcacggcaat
    42001 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    42061 cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    42121 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    42181 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    42241 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
    42301 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
    42361 ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc tggcagactg tggttagtca
    42421 gctctttcgc tagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
    42481 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    42541 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    42601 cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc agcgcccctt
    42661 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcacccggca
    42721 aacgaatgtc attttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg
    42781 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
    42841 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
    42901 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    42961 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    43021 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    43081 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    43141 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
    43201 cctggcaagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    43261 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
    43321 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    43381 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    43441 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    43501 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    43561 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    43621 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    43681 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    43741 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    43801 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    43861 ctcacagatt ttccctattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    43921 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    43981 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    44041 gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc gtacattttc
    44101 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggtgtgta
    44161 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    44221 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    44281 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    44341 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    44401 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    44461 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    44521 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    44581 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgcctgacgc
    44641 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    44701 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    44761 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    44821 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    44881 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    44941 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    45001 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    45061 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    45121 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    45181 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    45241 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    45301 ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    45361 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
    45421 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    45481 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    45541 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag
    45601 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    45661 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa atacaagttc
    45721 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    45781 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
    45841 accgctaatc tgggtaacgg catcgtcgct atcggctata aatctcagcc ggtactggtt
    45901 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    45961 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    46021 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    46081 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    46141 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    46201 cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta caaagctgag
    46261 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    46321 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    46381 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    46441 ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg
    46501 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtactg
    46561 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    46621 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    46681 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
    46741 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    46801 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    46861 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    46921 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    46981 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    47041 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    47101 tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc gattgccgat
    47161 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    47221 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa
    47281 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    47341 cagcttaatg acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    47401 ttgttgcgcg aagggatgaa agtagtgatt gccggacgtc ctaatgccgg taaatcgagc
    47461 ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc cggaaccacg
    47521 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacact
    47581 gccgggctac gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag
    47641 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt
    47701 gatccggcag agatctggcc ggaatttatc gcccgtctgc ccgcgaaact gccgatcacc
    47761 gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    47821 cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat
    47881 ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc gcgccgtcgc
    47941 cacttacagg cgctggaaca ggcagcggag catttgcagc aaggtaaagc gcaactgttg
    48001 ggagcctggg caggtgaact gctggcggaa gagttgcgcc tggcacagca gaacttaagc
    48061 gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt
    48121 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
    48181 ttgttagcac tcctgttatg cctcaaccct tttttaaaca ttaaaattct tacgtaattt
    48241 ataatcttta aaaaaagcat ttaatactgc tccccgaacg attgtgattc gattcacatt
    48301 taaacaattt cagaatagac aaa
//
LOCUS       DAJTUM010000021        47956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTUM010000021 DAJTUM010000000
VERSION     DAJTUM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO471_001294"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO471_001294"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6242627.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO471_001295"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO471_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6242628.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO471_001296"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO471_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6242629.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO471_001297"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO471_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6242630.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO471_001298"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO471_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6242631.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO471_001299"
     CDS             5377..5835
                     /locus_tag="OO471_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6242632.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO471_001300"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO471_001300"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6242633.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO471_001301"
     tRNA            6887..6971
                     /locus_tag="OO471_001301"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO471_001302"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO471_001302"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO471_001303"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO471_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6242634.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO471_001304"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO471_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6242635.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO471_001305"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO471_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6242636.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO471_001306"
     CDS             complement(10252..13995)
                     /locus_tag="OO471_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6242637.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO471_001307"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO471_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6242638.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO471_001308"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO471_001308"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6242639.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO471_001309"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO471_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6242640.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO471_001310"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO471_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6242641.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO471_001311"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO471_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6242642.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO471_001312"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO471_001312"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6242643.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO471_001313"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO471_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6242644.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO471_001314"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO471_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6242645.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO471_001315"
     CDS             23392..23499
                     /locus_tag="OO471_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6242646.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO471_001316"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO471_001316"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6242647.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO471_001317"
     CDS             complement(24538..25347)
                     /locus_tag="OO471_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6242648.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO471_001318"
     CDS             complement(25351..25743)
                     /locus_tag="OO471_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6242649.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO471_001319"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO471_001319"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6242650.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO471_001320"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO471_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6242651.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO471_001321"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO471_001321"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6242652.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO471_001322"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO471_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6242653.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO471_001323"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO471_001323"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6242654.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO471_001324"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO471_001324"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6242655.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO471_001325"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO471_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6242656.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO471_001326"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO471_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6242657.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO471_001327"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO471_001327"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6242658.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO471_001328"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO471_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6242659.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO471_001329"
     CDS             36790..39657
                     /locus_tag="OO471_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6242660.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO471_001330"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO471_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6242661.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO471_001331"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO471_001331"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6242662.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO471_001332"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO471_001332"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6242663.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO471_001333"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO471_001333"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6242664.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO471_001334"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO471_001334"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6242665.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYRDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO471_001335"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO471_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12042 a        12343 c        11742 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaaa ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcattac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct accgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAJTUM010000022        47386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTUM010000022 DAJTUM010000000
VERSION     DAJTUM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            80..562
                     /gene="sseA"
                     /locus_tag="OO471_001336"
                     /pseudo
     CDS             80..562
                     /gene="sseA"
                     /locus_tag="OO471_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1381..2157)
                     /gene="sseB"
                     /locus_tag="OO471_001337"
     CDS             complement(1381..2157)
                     /gene="sseB"
                     /locus_tag="OO471_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6242666.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2299..3582)
                     /gene="pepB"
                     /locus_tag="OO471_001338"
     CDS             complement(2299..3582)
                     /gene="pepB"
                     /locus_tag="OO471_001338"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6242667.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3641..3841)
                     /gene="iscX"
                     /locus_tag="OO471_001339"
     CDS             complement(3641..3841)
                     /gene="iscX"
                     /locus_tag="OO471_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6242668.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3853..4188)
                     /gene="fdx"
                     /locus_tag="OO471_001340"
     CDS             complement(3853..4188)
                     /gene="fdx"
                     /locus_tag="OO471_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6242669.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4190..6040)
                     /gene="hscA"
                     /locus_tag="OO471_001341"
     CDS             complement(4190..6040)
                     /gene="hscA"
                     /locus_tag="OO471_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6242670.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6057..6572)
                     /gene="hscB"
                     /locus_tag="OO471_001342"
     CDS             complement(6057..6572)
                     /gene="hscB"
                     /locus_tag="OO471_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6242671.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6668..6991)
                     /gene="iscA"
                     /locus_tag="OO471_001343"
     CDS             complement(6668..6991)
                     /gene="iscA"
                     /locus_tag="OO471_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6242672.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7008..7394)
                     /gene="iscU"
                     /locus_tag="OO471_001344"
     CDS             complement(7008..7394)
                     /gene="iscU"
                     /locus_tag="OO471_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6242673.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7422..8636)
                     /gene="iscS"
                     /locus_tag="OO471_001345"
     CDS             complement(7422..8636)
                     /gene="iscS"
                     /locus_tag="OO471_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6242674.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8748..9236)
                     /gene="iscR"
                     /locus_tag="OO471_001346"
     CDS             complement(8748..9236)
                     /gene="iscR"
                     /locus_tag="OO471_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6242675.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9506..10246)
                     /gene="trmJ"
                     /locus_tag="OO471_001347"
     CDS             complement(9506..10246)
                     /gene="trmJ"
                     /locus_tag="OO471_001347"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6242676.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10365..11168
                     /gene="suhB"
                     /locus_tag="OO471_001348"
     CDS             10365..11168
                     /gene="suhB"
                     /locus_tag="OO471_001348"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6242677.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11313..12167
                     /locus_tag="OO471_001349"
     CDS             11313..12167
                     /locus_tag="OO471_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6242678.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12358..13638
                     /gene="csiE"
                     /locus_tag="OO471_001350"
     CDS             12358..13638
                     /gene="csiE"
                     /locus_tag="OO471_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6242679.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13630..14769)
                     /locus_tag="OO471_001351"
     CDS             complement(13630..14769)
                     /locus_tag="OO471_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6242680.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14929..15819)
                     /gene="hcaR"
                     /locus_tag="OO471_001352"
     CDS             complement(14929..15819)
                     /gene="hcaR"
                     /locus_tag="OO471_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6242681.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15955..17316
                     /locus_tag="OO471_001353"
     CDS             15955..17316
                     /locus_tag="OO471_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6242682.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17313..17831
                     /locus_tag="OO471_001354"
     CDS             17313..17831
                     /locus_tag="OO471_001354"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6242683.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17831..18151
                     /locus_tag="OO471_001355"
     CDS             17831..18151
                     /locus_tag="OO471_001355"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6242684.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18148..18960
                     /gene="hcaB"
                     /locus_tag="OO471_001356"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18148..18960
                     /gene="hcaB"
                     /locus_tag="OO471_001356"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6242685.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETPIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18970..20197
                     /locus_tag="OO471_001357"
                     /pseudo
     CDS             18970..20197
                     /locus_tag="OO471_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20294..20716
                     /locus_tag="OO471_001358"
     CDS             20294..20716
                     /locus_tag="OO471_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6242686.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20764..21636)
                     /locus_tag="OO471_001359"
     CDS             complement(20764..21636)
                     /locus_tag="OO471_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6242687.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21648..22742)
                     /locus_tag="OO471_001360"
     CDS             complement(21648..22742)
                     /locus_tag="OO471_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6242688.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22775..23773)
                     /locus_tag="OO471_001361"
     CDS             complement(22775..23773)
                     /locus_tag="OO471_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6242689.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23798..25309)
                     /locus_tag="OO471_001362"
     CDS             complement(23798..25309)
                     /locus_tag="OO471_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6242690.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25325..26319)
                     /locus_tag="OO471_001363"
                     /pseudo
     CDS             complement(25325..26319)
                     /locus_tag="OO471_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26416..29517)
                     /locus_tag="OO471_001364"
     CDS             complement(26416..29517)
                     /locus_tag="OO471_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6242691.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29524..29697)
                     /locus_tag="OO471_001365"
     CDS             complement(29524..29697)
                     /locus_tag="OO471_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242692.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29815..31008
                     /locus_tag="OO471_001366"
     CDS             29815..31008
                     /locus_tag="OO471_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6242693.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31206..32459)
                     /gene="glyA"
                     /locus_tag="OO471_001367"
     CDS             complement(31206..32459)
                     /gene="glyA"
                     /locus_tag="OO471_001367"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6242694.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32786..33976
                     /gene="hmpA"
                     /locus_tag="OO471_001368"
     CDS             32786..33976
                     /gene="hmpA"
                     /locus_tag="OO471_001368"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6242695.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34021..34359)
                     /gene="glnB"
                     /locus_tag="OO471_001369"
     CDS             complement(34021..34359)
                     /gene="glnB"
                     /locus_tag="OO471_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6242696.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34420..35754)
                     /gene="glrR"
                     /locus_tag="OO471_001370"
     CDS             complement(34420..35754)
                     /gene="glrR"
                     /locus_tag="OO471_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6242697.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35744..36457)
                     /gene="qseG"
                     /locus_tag="OO471_001371"
     CDS             complement(35744..36457)
                     /gene="qseG"
                     /locus_tag="OO471_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6242698.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36622..38049)
                     /gene="qseE"
                     /locus_tag="OO471_001372"
     CDS             complement(36622..38049)
                     /gene="qseE"
                     /locus_tag="OO471_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6242699.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38607..42494)
                     /gene="purL"
                     /locus_tag="OO471_001373"
                     /gene_synonym="purI"
     CDS             complement(38607..42494)
                     /gene="purL"
                     /locus_tag="OO471_001373"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6242700.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42753..44309
                     /gene="mltF"
                     /locus_tag="OO471_001374"
     CDS             42753..44309
                     /gene="mltF"
                     /locus_tag="OO471_001374"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6242701.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44306..44842)
                     /gene="tadA"
                     /locus_tag="OO471_001375"
     CDS             complement(44306..44842)
                     /gene="tadA"
                     /locus_tag="OO471_001375"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6242702.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44867..45502)
                     /gene="yfhb"
                     /locus_tag="OO471_001376"
     CDS             complement(44867..45502)
                     /gene="yfhb"
                     /locus_tag="OO471_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6242703.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45711..46559
                     /locus_tag="OO471_001377"
     CDS             45711..46559
                     /locus_tag="OO471_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6242704.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46898..47386)
                     /locus_tag="OO471_001378"
                     /pseudo
     CDS             complement(46898..47386)
                     /locus_tag="OO471_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11301 a        12982 c        11926 g        11177 t
ORIGIN      
        1 catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg
       61 ggtacgaata aagtaacggg gacttgcagg ctggcagcgc gatgacctgc tattagaaga
      121 aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg aagcagtggt
      181 gaaagtaacc gatgtgttat tggcaagcca tgaaaatacg gcgcaaatta ttgatgcccg
      241 cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac gtcgcggaca
      301 tattcccggt gcattgaacg ttccgtggac ggaactggtg cgcgaaggcg aactaaaaac
      361 gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctatgaca aaccgattat
      421 cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg cgacgctgga
      481 tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc gggcagattt
      541 accggttgag ccagtgaaat aagtatatta caggcaataa aaaaccgccg aatttggcgt
      601 ttttttattg ctggtctggt tcgcggcctt tccagcaggt tgacttgtgt tacatgagca
      661 acgcaggtgc ttcacagcaa aacaatactc accagtaact ctctttttgt caagcaaaag
      721 agagtaatta ttgtttattt agcgtattat cgacaccggc cctttccgcc gtgttcggta
      781 ataaaataac ctggcttatt agtccgaatt cagacaaata taaacaaatc ctgctcaaaa
      841 ttaaaaattc taaccggtaa aagatattac ttaaacatgt aaatttactt tcctttaaaa
      901 aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg tccggttcgc ggcctttcca
      961 gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa
     1021 ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt atccactgcg
     1081 gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt
     1141 agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat
     1201 gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta cttattgttt
     1261 ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac aatttactgg
     1321 tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg gcggcatatt
     1381 ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac gttcatagaa
     1441 tggcgcaata tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca
     1501 aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg tcgcctgaat
     1561 aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc
     1621 gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt taaataaggt
     1681 ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata tcagcgattc
     1741 accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa tcaacaaact
     1801 gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga agagcgtttc
     1861 gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct gttcgtcttc
     1921 aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc
     1981 tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc cctgcgccgc
     2041 cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg ggcggtgcgc
     2101 cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt cggacataag
     2161 aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct tatcaggcct
     2221 acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag gcgtttacgc
     2281 cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca caccaagtcc
     2341 cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg agcagtcgat
     2401 atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg ccgccgtgct
     2461 cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt tagacggcag
     2521 ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct cttgtgaggc
     2581 actcgccagt aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt gataatcatt
     2641 acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca tttccggttt
     2701 ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg catcagtgtt
     2761 catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct tgaacgcatt
     2821 gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt tcagtccgcg
     2881 cataatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat
     2941 cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag tgataccttt
     3001 acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt aatccagcgc
     3061 cagcaatacc ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca tataaccttg
     3121 ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg cgacgttgct
     3181 gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg ccggtgcgtt
     3241 gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct ggcgctgggc
     3301 atcgtccaga tccggccact ccactttacg cgtgcctttc gggcctttat aaccttgcca
     3361 gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat gcttgatgcc
     3421 cagaccgtca atcttgcggg ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa
     3481 atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc agcgcgcgtc
     3541 ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta tccttattcg
     3601 tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct tcgtccagcc
     3661 agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat
     3721 cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc ggatcaagat
     3781 cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac ttaagtccca
     3841 tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga tttcgaccac
     3901 taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct ccagtcccca
     3961 ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt caaaaccttc
     4021 acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt gttcaatctc
     4081 aataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt ccagaacagc
     4141 gccatcaggg cagaggtcct gatgaggcaa aataacaatc tttggcatat taaacctcgt
     4201 ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga gcggcgaaat
     4261 cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct
     4321 gcgccacttc actcaggtga gcggcagcat tgtcaatgac ctgacgctct gcggcgctta
     4381 acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc gccgcttcta
     4441 ctttttgttc tgccagcata cgggctttta cgtcctgctc ggcatagctc attgagtctt
     4501 tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc tgaatagacg
     4561 cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct
     4621 ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa
     4681 aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt acatggatag
     4741 acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag
     4801 tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg cccagcgaca
     4861 gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc agaatatccg
     4921 cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc agcggtggac
     4981 gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa ccgcccacca
     5041 tcaccacttc cagcacttcg tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag
     5101 ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca cggctgattt
     5161 cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc gcgattttgg
     5221 ctgcaatggt ggcatccagc agttcacgct gaacgcggtt atcgctacga tcaggaatgt
     5281 ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg
     5341 aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga atggaaatat
     5401 caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg gaatccagcc
     5461 cgtaggcgat agccgcagca gtcggttcgt taagtaagcg cagaacatgc aaccccgcca
     5521 gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga
     5581 taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc agtgctttga
     5641 ggatgtccgc agaaacgcgc accgggttca gtagccccgc cgccgtttca atcatcggca
     5701 ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc tggatatcag
     5761 ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg tcaagcgctg
     5821 cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga acaacagatg
     5881 gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg
     5941 ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc tgatgcggcg
     6001 cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc cagaaattaa
     6061 aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc gagaaaacgc
     6121 agcttacgca ccgtatccgc cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc
     6181 aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc cagccgcgct
     6241 tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg ttccatcagg
     6301 aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg taaagaaagc
     6361 aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt aatggttgca
     6421 gattgctgta cggcggcgag ttgttccgcc aggcttccgc tggcgaattt atcaggatga
     6481 tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg
     6541 gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg atccgccgcg
     6601 ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg ggttatcggt
     6661 atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg ttcgggttgg
     6721 tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta ccgtccagaa
     6781 attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac acgatgtctt
     6841 ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat ccggaggttc
     6901 tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta tttactcgcg
     6961 ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta ttttgcttca
     7021 cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag aatagaacag
     7081 tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt gatcgcctgc
     7141 gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga agcgatagcg
     7201 gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc gttgacttta
     7261 atctgcaact tcattacgtc gccacaggcc ggtgcaccca ccatgccgct gccgacgttc
     7321 tcgtcgttgt tatcaaagga acccacgtta cgcggattct cgtaatggtc gataactttt
     7381 tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg agcccattcg
     7441 atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag gtcacgcaga
     7501 cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc agtagtaaaa
     7561 cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc cagcgcgcgc
     7621 agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga aactgcgagg
     7681 tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac gttgagaatg
     7741 ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc tttgatgccg
     7801 ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat ctcttctttt
     7861 gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt gccggaacgc
     7921 ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga
     7981 cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga gaaagacatc
     8041 aggtcaactt tcaactggct caggtcgatt ggcagtttgc ccacgctctg ggttgcatca
     8101 acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat atcctgcacc
     8161 acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc
     8221 gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta ggtgacttcg
     8281 aaaccttcgc gctccagctg acggcaagta tccagtaccg ctttgtgttc ggttttgctg
     8341 gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat cgccaggttg
     8401 tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg
     8461 gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa acggtgagaa
     8521 cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga actgcatcat tttctcggca
     8581 acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa tttcattgct
     8641 ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga tgtacgaccg
     8701 tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta agcgcgtaac
     8761 ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca
     8821 gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt gttgagaaaa
     8881 ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc
     8941 tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac ggcgctaatc
     9001 acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
     9061 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
     9121 agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg
     9181 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
     9241 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
     9301 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
     9361 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtcggat
     9421 gcggtgtgaa caccttatcc gacctacgat tctgtacatt gtaggcctga taagacgcgt
     9481 taagcgtcgc atcaggcaac cactgttatt cggccttgtt acctttattc tgctgctcaa
     9541 tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc gcacgggtaa
     9601 acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata aaaccggttg
     9661 ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc agcggatacg
     9721 gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag gccatacgca
     9781 cttcataggc aataacctga accgccattg ccaggttcag cgaactgtat tccgggtttg
     9841 ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag ccgacgcgct
     9901 cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt ttcagaccgc
     9961 attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca
    10021 ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca
    10081 catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc agtggattaa
    10141 ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa cccatattgc
    10201 cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc tttcttcatc
    10261 taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc gctgctatac
    10321 tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat ccgatgctga
    10381 acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac tatgaaaccc
    10441 cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac gtagataaag
    10501 ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac accatcatca
    10561 ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt atcgatccac
    10621 tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct atcgccgttc
    10681 gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac gaactgttca
    10741 ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt agcaccgctc
    10801 gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca
    10861 ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg
    10921 gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa
    10981 tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca
    11041 tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc gttgctggta
    11101 acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc gacgctctga
    11161 agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca ggcgctattc
    11221 cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct ttccccgatt
    11281 aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac aaacgcgttc
    11341 ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg
    11401 ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc tggtcggcgg
    11461 aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt attccttctt
    11521 cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc aatgccggaa
    11581 atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc aacgttttta
    11641 tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg
    11701 acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc
    11761 tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt ggtcagggtg
    11821 atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat gcaaccatcg
    11881 ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc ggcgaaaatt
    11941 acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc gatcacgtta
    12001 tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa cggttgatcc
    12061 taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat gtttatcgcg
    12121 aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc atgatcgcta
    12181 gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc agaacagttt
    12241 aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat tcccttcgcc
    12301 atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga tctacagatg
    12361 atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg ccagatcttg
    12421 ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga gcttaatggt
    12481 gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat cctgcgctat
    12541 catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg tacagtgctt
    12601 aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg tccgtcattt
    12661 attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg aattgcgcgt
    12721 aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg acggctgcaa
    12781 aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata ttgtttgctg
    12841 cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc
    12901 tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag
    12961 catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt gcgggttccc
    13021 gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat caaacgtctg
    13081 gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg gttatgtgat
    13141 cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat cggtattgat
    13201 aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac cacccgcgcg
    13261 gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga aagtggtctg
    13321 gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga aaaacagatt
    13381 attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa
    13441 ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt gcagacaggc
    13501 gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc gctcttttca
    13561 ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca gatccgcaaa
    13621 atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg gaagcgccac
    13681 cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac cggcgaaaac
    13741 ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct gtaaacggat
    13801 gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc agaccgtgaa
    13861 ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag ttgctcccat
    13921 aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc gagcactaca
    13981 acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca cgcccagcga
    14041 ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa tggcgctaaa
    14101 accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa agcgccagtt
    14161 ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc ggcttgcccc
    14221 ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca cgcccaacgt
    14281 caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg tcagcgccga
    14341 gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa gcgggaactg
    14401 cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga aaaagaggtt
    14461 aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc
    14521 aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg aaggatcgct
    14581 gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg cccccaataa
    14641 caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa
    14701 aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc gcgtggattg
    14761 caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga atgttggtgc
    14821 aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag
    14881 cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac
    14941 gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagct tcatctcccc
    15001 tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg gacgaaaaac
    15061 aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc ccagccccat
    15121 gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat ttggctggct
    15181 gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat acgccggatc
    15241 ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt catgtgctaa
    15301 agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca gataatcaat
    15361 ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt
    15421 ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg gctgtctgag
    15481 acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca caaagccaat
    15541 ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat tttccgcttg
    15601 ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca acgcgacttt
    15661 gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc ggatctggct
    15721 gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg
    15781 cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct tgttatcgaa
    15841 aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac cagcaacatc
    15901 cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa aaatatgacc
    15961 acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg ggtcactccg
    16021 cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt cggtcgttgc
    16081 tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt taacacctac
    16141 atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc
    16201 aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa cagtcgcgcc
    16261 tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat cgatgtaccg
    16321 ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct aaacgaagtt
    16381 ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag cgcaccgggc
    16441 ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa
    16501 ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc
    16561 ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc ttctgccgtt
    16621 caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc
    16681 cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc
    16741 ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc agtttcaagc
    16801 tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc
    16861 ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac tgccacactc
    16921 cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg tattactgac
    16981 aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg tgcttttggt
    17041 cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca gaaattgctt
    17101 aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag
    17161 cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac tgccgctcgt
    17221 ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga agtgctcgat
    17281 aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca ctggagttac
    17341 atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac tggaaatttc
    17401 gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc acggttaacg
    17461 cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt ttcaatgaca
    17521 ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc tgggcagaag
    17581 agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa accgacatcc
    17641 caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg
    17701 aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat gacaactggc
    17761 gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat aacctgagtg
    17821 tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc
    17881 tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc
    17941 cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc tggaagatga
    18001 cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt
    18061 atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag gtggtgacat
    18121 tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc catttttatt
    18181 accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga agaaggcgcg
    18241 caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc
    18301 gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca acgcgcggtc
    18361 gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc aggcatctgg
    18421 gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg cttccacgag
    18481 ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta
    18541 atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta tcctggcggc
    18601 ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg ccaactggct
    18661 tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac
    18721 ctgcgcggcc cacaggcgct cgggcaaagt gaaaccccga taatgcagtc tctgacgccg
    18781 gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga ttttacgggg
    18841 ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac
    18901 gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct ggatctttaa
    18961 ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg
    19021 gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt ttccgatgag
    19081 cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga agattccccg
    19141 cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca tctgcattcc
    19201 ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac caacggcgaa
    19261 agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc gctgccgttg
    19321 cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc cgccagactg
    19381 cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac tattggtctg
    19441 gaactggctg ccagcgccac gcagcgcagc gcagcgcagc gcagcgcagc gcagatgtaa
    19501 ggtgacagtg attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca
    19561 acgctatctt ttacagcgcc atcagcaggc tggtgtgcgc attctgctca ataatgccat
    19621 tgaacatgtg gtcgatggtg aaaaagtaga actgacgctg caaagtgggg aaacgcttca
    19681 ggctgatgtg gtgatttacg gtattggtat cagcgccaac gagcaactgg ctcgcgaggc
    19741 caaccttgat actgccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc
    19801 gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg
    19861 cgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa tgttagggct
    19921 accgctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca
    19981 gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa
    20041 ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg
    20101 ccgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact
    20161 gctgatagat gagaacatcg cgcttaaatc actgtaatca ggataattag cgaatatctc
    20221 aatgcctggg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc
    20281 aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt
    20341 gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    20401 gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct
    20461 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    20521 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    20581 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa
    20641 gaatgtcagt atcgctggcg cgttcttgct attggcaatt accggaccgg gggcaatttc
    20701 tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat
    20761 aaattacagc cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag
    20821 cgcaatgaga tcaccccctt ccgggcgatg atgctcatcc ggcgcatggc tcatcggttc
    20881 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa
    20941 ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc
    21001 gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg
    21061 cgcgagctgg ttaaaatcca gttcctgcgg tagctgctcg caaaactcac ccgccagcca
    21121 ctgctcccgt tccagccagt aaccgctcgc ctgcgcctga atccgcgttt gtggtgacaa
    21181 cggaaaataa ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac
    21241 agagagcgtc accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg
    21301 atagacaccg ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca
    21361 ttgccactcg cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg
    21421 ttgcagttga tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa
    21481 tggcaccagc ggaaaacatg aggcatcggt tgccacaccg ctttttttac caggacgtaa
    21541 gagcggcgtc gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgtccag
    21601 ctttagcgac ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag
    21661 ttaatcacaa ctttcccgca tttgccgctc gccatcagca cataggcatc acctgcctgt
    21721 tctagcgaga agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg
    21781 tgggcgcatt tttccatatg gaacagactg gtcacccagg agccgataat ccgccgttga
    21841 tggtgcatca gatcagcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc
    21901 acccgccccc agtcagcagt ggattgcagt gccagcaagc gacctgcggc attaccggaa
    21961 caatcgagcg caacatccgc gccaccgtgg gtaagttcag caattatctg cggcagacct
    22021 tcggtggttg ctaaatagcc gtgatccatc ccccctaact gttttgccat cgccagacgt
    22081 tccggcagca tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc
    22141 gccatcatgc cgaccggccc cagaccgacc accagcacgt tatcgctgcc ggaaacttcg
    22201 ccgcgcaaaa tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg
    22261 tagctcagcg catccggcag gaggatcaga tctttttctt ccgccagcag atattcggca
    22321 tgaccgccgt cacgctgcca gccgtaagcc gcttttcctt tgccagtaca agagatagga
    22381 aagccgcggc ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg
    22441 tcgccctctt taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca
    22501 tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt
    22561 tgatgataga tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt
    22621 acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt tgaatttcct
    22681 agtaaataag cagccagcat cgttttcata aatccctcat taacaatacg attaattttc
    22741 atccctgcag cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgcttcgct
    22801 gggtcagcaa gatattcgcc agcaccgcca ccacgatgat gacgccgcgt accacctgct
    22861 ggaaaaagga gttaataccg agcagcacca gaccgttacc gattagcgta atcaccagca
    22921 caccaagcaa tgtaccgaac agggaaccgc gaccgccgga aagtgccgta ccgccgacca
    22981 cgaccgcggc gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga
    23041 ggcgtgccgc caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa
    23101 gaatgcgcac tcgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa
    23161 cggcaaaaac cgagcgcccg aaggcggttt ttcggctaat gaacacaaac agcgcaaaca
    23221 acaccatcat gatcagcgcg gatactggca caccgagaaa ttgtccgccc agccagtcca
    23281 gcacctcgtt ttcgtcaatc ggcactggca gtgcgttggt cataaacagc cccattccgc
    23341 gcagagcgct ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca
    23401 gcaccccggc gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg
    23461 ccaacggaac ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg
    23521 gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa
    23581 tcccaatggt ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag
    23641 gcgcgttcag ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat
    23701 taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg
    23761 acgaagcata catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg
    23821 acatcagctc atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta
    23881 ataacaggat gcggtcacac accagcggca attcctccac ttcactggag ataaacacca
    23941 cgctttttcc ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga
    24001 tatcgacgcc gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac
    24061 gaccgatcac cactttttgc tgattgccac cagaaagcgt gccgatgggt gtttcgctac
    24121 tggcagcctt gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc
    24181 attgcagcac accgttggtg ctgatttttt gccgattggt cagcactgta ttttcgtcaa
    24241 cgcctagcca gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc
    24301 gtttcagcat gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc
    24361 cctgttcata cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca
    24421 gcaggccagc aatgccaagc acttcgccac gacgtagcgt aaagctgata tcctccagct
    24481 tgggcttatg gcgtaacgca cggacttcca gtacggcctg atccacaatt tcctgagggg
    24541 caaccggcgc aatatcaacg tgatcacgcc ctagcattag cgacacaata tgatgcgtgg
    24601 aggtgttttc gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcatagg
    24661 aggcaatgcg gcggatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg
    24721 ccgacatctt tttcaccgca ctgatcacca gttcaacttc tgcactggca agcgagctgg
    24781 tgggttcatc aagaatgacc acgcgcggct cgcccttcat cacccgtgca atttccacca
    24841 gttgcttttg cgccgggctt agcgttgaaa caagttgttc aggactaacg tcaacgccca
    24901 gcgcctgtaa gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc
    24961 ggcgaggcca ttgaccaagg cagaggtttt ctgccaccgt cagcccttcc accagactta
    25021 attcctgata aaccgcacga acccccagtt cagcggcacg gcgagtcaac gtagcttcgt
    25081 caccttccag tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt tcgctgccgg
    25141 taagcattcg aatgagagtc gatttgcccg cgccgttttt acctaacagc gcacgaactt
    25201 cgcctttatt gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat
    25261 ttcctgccac cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg
    25321 cgaatcagcg attagggcag agcagaccat caacgtgcgt tgccagccac tgtttaccat
    25381 cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg tcggcttgtt
    25441 tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta ccggaaatat
    25501 ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg gttgtcatat
    25561 ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct tttaccgcgc
    25621 cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg gagataatca
    25681 gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta gcgacaattt
    25741 gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc tgcacacaaa
    25801 cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta ttggcaataa
    25861 aataatcggc ggcagcgtta cccagttttt accaaattcc agtggatcgc cgaccagatc
    25921 cgccgagaca tatttatcga cacccttttg attaatacag gtgttgtagc aaatcaccgg
    25981 aatacccgct tcactggcgc gacgaacggt acggctactg ccattttcag acactgccga
    26041 taaaataatg gcatcgacat tacgcgccac gagggtatca acaaaggtac tttctttcga
    26101 aatatccccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat cttttgccgc
    26161 atcctgaacg ccctgacgca ctccagcgta atatccctgg gtatcaaggt atattgcacc
    26221 aatggtcatt tctttatccg cagccctggc aaatagtgcg ctacctaata gcgtagccat
    26281 taataataga ttacgggttg ttctcatttt tttaggcata gtgcctcctg tagggttttt
    26341 attaacaacg gcttattcta attattttgt gatgagcggc agcgcgtgcc gctcaaatat
    26401 tacagttaat agggtttagt gaacgtagtt aaaaataaaa ggcatcacag tggtgaataa
    26461 tgccgctttc ggccaggccg gattaatcgc cgtcaattca gcgcgagcag actcatattg
    26521 cgcaacctcc ccgagtcccg cggacgccag catcgccacc atcaggcatt tttctttatg
    26581 ctgctgttgt aaatcgccat acagcgataa caggtcaggt tgtgaaacag caaagaaatc
    26641 cgcctcaata ctggttttcg ccatctcttg cgcccactgt ttcatttcac taaacagttg
    26701 ctgtgcggtt tgctgttcac ccagcagtcg cagcgccatt ccttgccaga agagataatc
    26761 aaccggctga tcgttgtaat aactgtggat gttaatggtg cgatcgccgg tcgccgccag
    26821 acgtaaacaa cgcatcgctt cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca
    26881 gaaccagatg tcgttatcag tttgccccgg taaacggcct tcgcttaaat tctccggata
    26941 atacagcgcg gcatgaagca gttcgctggc ctgctgcggc tctctggcat caagatgctg
    27001 ccaggcgcgt aataactggt tgaggataaa ctgactggtg accttccctt ccccgccttc
    27061 ccacgggtgg aatttacgcg tggcgagaat gtccgccgct ttgtctgcct gacccgtgag
    27121 atgccacaaa ttgagcagtt ctgcggtcat gtcgtcgcgt ttcagcgcaa tttccagatt
    27181 attttccaga cgcgccagtc gtttctccgg tgtggctcca cttaacttat ccagcaaatc
    27241 ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat caagataacg
    27301 cgcggccagc tcataatcgt gtttcttatt ccacgcatgg atcgctaacc cgcgccagcc
    27361 gtcggcaaac tccggcgaca tctctacgca acgttgccat aaggcaatgg ctttgttgta
    27421 gctacgtttg ttgtagtaga agcaggccag taaatggcga gcaaaccaac actcttcaat
    27481 actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact gcgggaagac
    27541 atcaatggct tttgcgacca gttcgccacg ttcggctttc ggcagcaagc tggcttgcag
    27601 gtaaagcggc agcgtgcgct ggcagtccag tgcgttcagc atctctgccg ccagggtggg
    27661 cattccccag ttaatcagtt gcccggcggt catcagagcg ttaacgtcgc gtccctgaca
    27721 cagaccgcgc cactgcgcga gggctgattc gctacgacca tcgaaccagt tcagccacca
    27781 cagagtggcg ttcagcggat aatcacgcag cagtttctcg cgctgcaaac gcgcgttgtc
    27841 ctgacgacca cttaacacca gcagcagatt atgcaagcaa agcacttcct gattggttgg
    27901 gcaggcacga agactttgtt ggcaaaaatc cagacccgcg tcgaagttat cattacgcgc
    27961 cgccagtcgt gccagaccat aatagccgcc tgctttacta ttgccgctcc agaccgcacg
    28021 ccagaaatcc tcttcggctt gttgatattg tccctgacgt tcgtaagcac tggcgcgaat
    28081 caaacttgct tgtccacact gcggtttttt gttcagcgca tgtgcacgtt tcagcgcctg
    28141 actggcatac gccaccgctt gcgggaaatc tgcgcggtta tattccagca tcgccagcgc
    28201 caagttacag cgataatcca gcgggtccag cgccacgccg cgcaggtagt aatcgaacgg
    28261 tgaacggctg gcgtgatgat attgctccag atgctgaccg ataaaccagg cttcatctgt
    28321 actggtaatg tcttgtgctg ccagtggcgc tttggcgacg tccggcaacg gcaactcctg
    28381 cggctgatgt tcctgataac tcagtacaat attgccgtcg gcatcggaga gttcaatggt
    28441 cagtctgtca ggattgatac cgtgcaacac gccctggatg gcggtcgctg gtgtcagggc
    28501 cacggcatca tcgagcaacg cgttgcattt gccgatttcg cggatcgcca gacggtatcc
    28561 gttcaacgga gagatggcat acagccccca ctcaatcccc cgctcactac gctggagttt
    28621 tatcaccgca tcgcgggagg cattttgcac catgcccaga gaatgataag gcaggaaata
    28681 ctgctcgaaa cgcttctctt cgtaagcatc aagccaggta aaatcaggct ggttatcggc
    28741 aaaaataccg gtcatcagtt cgatatacgg gccgttattg tcggtcagac tcttatccca
    28801 cgcctggcca aattcactgt gtccccaact ccactgtttt ttacctggcg caatatggtg
    28861 gttggcaacg tgcagcaaac cgccatcttc atcgtgacac cacgcgccga caaaatcgta
    28921 ctgcgatttt tcggccatat atgaggttgg aacaggcaca tttttatagc gagaaatgtc
    28981 cactccggcg gagtagtcca ctttgtagta agtgccggtg gcgatgggga aagcggagac
    29041 ggcccgtttg ccgtgatcaa acaccgcagt cacatccggt gggaaaacgc tctgatgccc
    29101 ttcaccccct ttcactgccg ggttgaccca ccacaagaaa tgacgcggcg tggcgttgcc
    29161 gttatagacg cggctggcga tttccagtgc cgcccggtca gggcgcaggg tgaaacctgt
    29221 catcacctgt aaaccatgca tcggctccgt ttcgccgacc cacaccgtct gtgcaccgtc
    29281 ttcatgggct tcgagggtga aatcaacggg cataaaggtg gtcgggcgat ggtgttgcgg
    29341 ccagttaaac tcaatcccgc cggagatcca cggtcccagc atccccacca gcgcaggttt
    29401 aatgacttca ttgtgataga caaaatcgcg ctgtttcact ttatcccatg cgcgatgcac
    29461 ccgaccgccc agttccggca ggatcatcac tttgatgtag tcgttttcca gccacaccgc
    29521 ctgctaggat ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt agggataaac
    29581 cgcgccggac gatccctgat aaacgcgatt ttccaggaac atgggatgta tatcctgcgg
    29641 cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg gagtcatggt
    29701 gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta ttgaacaatc
    29761 tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt gaagatgaga
    29821 gcctgcatta acaatcaaca gattcgccac cataacaaat gcgtgattct ggaactgctg
    29881 taccggcaaa agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat ttcgattccg
    29941 gcagtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa tattgacgat
    30001 gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga aggtgactgg
    30061 acgctgtgcc tgaacgtgac gcccaccagt attgagtgtc aggttgctaa tgcttgttta
    30121 agtccgaaag gcgaatttga gtatttacag attgatgtac cgacgccgca ggcgctgctg
    30181 tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg caccatcaat
    30241 ctggcgctgg caatccacgg gcaggttgat ccggtgaccg gcgtatcgca aaccatgccg
    30301 caagcgccgt ggacaacgcc agttgaggtg aagtatctgc tggaagagaa gctgggcatt
    30361 cgggtgctgg tcgataatga ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat
    30421 tcgcaggaac gggatttctg cgtgatcaac gttgattacg gcattggctc gtcgttcgtg
    30481 attaacgagc aaatttatcg cggcagtttg tatggtagcg gacagattgg tcacaccatc
    30541 gttaatccgg atggagtcgt ctgcgactgt ggacgttacg gcagcctgga aaccgtcgcc
    30601 tcgttaagtg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc ggttaatact
    30661 cagcttgagc ctgaaaaact gacaaccgcg cagttaatcg ctgcctggca gagtggagaa
    30721 ccgtggatca ccagctgggt tgatcgctct gccaatgcca ttggtttgag tctgtataac
    30781 ttcctcaaca tcctcaatat taatcagatt tggttgtacg gtcgcagttg tgcctttggt
    30841 gagaactggc ttaatactat tattcgccag acaggattta acccgttcga ccgcgacgaa
    30901 ggaccgagcg tgaaagcgac gcaaattggc ttcgggcaat taagccgcgc acaacaggtg
    30961 ctgggaattg gctatttgta tgttgaggcg cagttacgac agatttgatg gcgcgataac
    31021 gtagaaaggc ttcccgaagg aagccttgat gatcataaac gaaaaattgc ctgatgcgct
    31081 acgcttatca ggcctacacg gagattgcaa tatattgaat ttgcaagatt ttgtaggcct
    31141 gagaaggtgt tcatgccgca tccaacatga acaacgagca cattgtcagc aaatcaccgt
    31201 ttcgcttatg cgtaaaccgg gtaacgtgcg cagatgtcga gaactttacc tttgatgcgc
    31261 tcgataacgg cttcatcatt gatgctgtcc agcacgtcac acatccagcc agccagttct
    31321 ttcgcttcgg cttctttaaa gccgcgacgg gtaatcgccg gagtacctac acgaataccg
    31381 gaggtcacaa acgggctctt cggatcgttc ggtacgctgt ttttgttgac ggtgatgtta
    31441 gcacggccca gagcggcgtc tgcttcttta ccggtcaggt ttttatcaac cagatcaacc
    31501 aggaacaggt ggttatcagt gccgccggaa accactttgt agccgcgctc gaggaacact
    31561 tctaccatcg ctttagcgtt tttcgcgacc tgctgctggt aagttttgaa ctcaggctcc
    31621 atcgcttctt tcagagcaac cgctttaccg gcgattacgt gcatcaacgg accgccctga
    31681 ccaccaggga aaacggcaga gttcagtttt ttgtacagct cttcgctacc acctttcgcc
    31741 aagatcaggc cgccgcgcgg acccgccagg gttttgtgag tggtggtagt aacaacgtga
    31801 gcatgaggaa ccgggttcgg gtagacgcca gcagcaacca ggcccgcaac gtgcgccata
    31861 tcaacgaaca gataagcacc gatgctgtca gcgatttcac gcattttcgc ccagtcaacc
    31921 acgccggaat atgcagagaa accaccgata atcattttcg gcttgtgttc tttggcttgt
    31981 ttttccagat cggcgtaatc gatatgaccg gtagcatcga taccgtaagg aacgatgttg
    32041 tacagtttac cggagaagtt aaccggagaa ccgtgagtca ggtgaccgcc atgcgccagg
    32101 ttcataccca gaacggtatc acctggttcc agcagcgcgg tgtagaccgc aaagttagcc
    32161 tgggagccgg agtgcggctg gacgttagcg tagtcagcgc cgaacagttc tttcgcacga
    32221 tcgatcgcca gttgttcaac gatatcaaca tactcgcaac cgccgtagta gcgtttgccc
    32281 ggataacctt cagcatattt gttggtcagc tgagaaccct gcgcctgcat tacgcgcggg
    32341 ctggtgtagt tttcggaggc gatcagttcg atgtgctctt cctgacgtac tttttcctgc
    32401 tccatagcct gccacagttc ggcatcataa tcggcaatgt tcatttcacg ctttaacatc
    32461 cgcatctcct gactcagcta acaataaaat ttttggcctt tataggcggt cctgttggac
    32521 aacggcgaac agtataaccg aatcattgtg cgataacagg tcttgacaaa ggaatttacg
    32581 caaacgatta ccttcaggct acgcaaggct ttggagaata aagagcttgc aaccggaaac
    32641 ggatttcttt tcaggtttgt gatgcaaatt tttcacttca tcacattctt tctgaaaaac
    32701 accaaagaac catttacatt gcagggctat tttttataag atgcatttga gatacatcaa
    32761 ttaagatgca aaaaaggaag accatatgct tgacgctcaa accatcgcta cagtaaaagc
    32821 caccatccct ttactggtgg aaacggggcc aaagttaacc gcccatttct acgaccgtat
    32881 gtttactcat aacccagaac tcaaagaaat ttttaacatg agtaaccagc gtaatggcga
    32941 tcaacgtgaa gccctgttta acgctattgc cgcctacgcc agtaatattg aaaacctgcc
    33001 tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac accagcttcc agatcaaacc
    33061 ggaacagtac aacatcgtcg gtgaacacct gttggcaacg ctggacgaaa tgttcagccc
    33121 ggggcaggaa gtgctggacg cgtggggtaa agcctatggt gtactggcta atgtatttat
    33181 caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa gccggtggtt gggaaggtac
    33241 tcgcgatttc cgcattgtgg ctaaaacacc gcgcagcgcg cttatcacca gcttcgaact
    33301 ggagccggtc gacggtggcg cagtggcaga ataccgtccg gggcaatatc tcggcgtctg
    33361 gctgaagccg gaaggtttcc cgcatcagga aattcgtcag tactctttga ctcgcaaacc
    33421 ggatggcaaa ggctatcgta tcgcggtgaa acgcgaagag ggtgggcagg tatccaactg
    33481 gttgcacaat cacgccaatg ttggcgatgt cgtgaaactg gtcgctccgg caggtgattt
    33541 ctttatggct gtcgcagatg acacaccagt gacgttaatc tctaccggtg ttggtcaaac
    33601 gccaatgctg gcaatgctcg acacgctggc aaaagcaggc cacacagcac aagtgaactg
    33661 gttccatgcg gcagaaaatg gcgaagttca cgcctttgct gatgaagtta aggaactggg
    33721 gcagtcactg ccgcgcttta ccgcgcacac ctggtatcgt cagccgagcg aagccgatcg
    33781 cgctaaaggt cagtttgata gcgaaggtct gatggatttg agcaaactgg aaggtgcgtt
    33841 cagcgatccg acaatgcagt tctatctctg cggcccggtt ggcttcatgc agtttaccgc
    33901 gaaacagtta gtggatctgg gcgtgaagca ggaaaacatt cattacgaat gctttggccc
    33961 gcataaggtg ctgtaatttg atgttgccgg atggaaacat ccggcaaccc ttgacgcggt
    34021 ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga atgacccgag ccacgtcaaa
    34081 gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc gtgcgaataa tggtatcgac
    34141 acaggtatcg acaatgtcgt ccggtacgac aatctcaatt ttcactttcg gcagaaaatc
    34201 caccatatac tccgcgccgc ggtacagctc ggtatggcct ttctggcgac caaagccttt
    34261 cacttcggtc accgtcatgc cggtaatacc gacttcggcc agtgcttcgc ggacatcgtc
    34321 cagcttgaag ggttttataa tcgcatcaat ctttttcatg ctattccttg aaaaggttgc
    34381 ctgtcttttg atctgctaaa cgtaacacat aacgccaatt cattccttga aatcgtttgc
    34441 atccagctcg tgtcgggaaa gcagtttata aaattctgtc cggttgcgcc ccgccattct
    34501 cgccgcgtgg gtgacgttgc ctttggtgat ttgcagcagc ttacgcaagt agttgagttc
    34561 aaaatgatta cgtgcctcaa caaaggttgg cagcgccgta ttttcaccct ccagcgcctg
    34621 ctccaccagc gcatcactaa tcaccggaga tgaggtcagc gccacgcact gttcaatcac
    34681 gttgaccaac tggcgcacat tacccggcca gctcgcggtc atcaggcgtt tcatcgcatc
    34741 ggtagagaac gcgcggacaa acggtttatg tcgctctgcc gcctggcgca acaggtgatt
    34801 tgccagtagc ggaatgtctt ctgtgcgctc cgccagtgcc ggaattttca ggctgacaac
    34861 gttgaggcgg taatagagat cttcgcggaa ttccccgcgc gccatcgctt ttgacagatc
    34921 acggtgagtg gcagaaataa tccgcacatt gatatcaata tcgcggttac tgcccagcgg
    34981 gcgcacttta cgctcctgca acacgcgcag cagtttgacc tgtaacggcg caggcatatc
    35041 gccaatttca tcgagaaata gcgttccgcc ttccgccgcc tggaataaac cttcgcgatt
    35101 gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac agctccgact ccagcaattg
    35161 ctcgggcaat gcgccacagt taatagcaat aaatggtttg ctgttgcgcg ggctggcgtt
    35221 gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc tgaccgttaa tcaaaacgct
    35281 gacgtctgat tgcgccacca gccgcgcctg ttccagcaaa cgcagcatca gcgggctgcg
    35341 ggtgacaatt gcctcgcgcc agcgttcatc ggtggctggc gcggattgct ccagcgcatc
    35401 gtcaattgcc tgatatagcg catctttatc gacaggcttg gtgaggaaac taaaaacgcc
    35461 ctgctgtgtt gcagcaacgg catcggggat agaaccatgc gcagtaagaa taattactgg
    35521 cattcccggc tgcactttct ggatttcagc aaatagctgc ataccgtcca tttcatccat
    35581 ccgcaggtcg ctgataacta aatctacttt ttcgcgattc agtacccgta atccttcagc
    35641 gccactttcc gccgtgacca cactgtagcc ttcgctggtc aggcgcaggc caagcagttt
    35701 cagcaatccc ggatcgtcat cgaccaataa taaatgcgca ggtttatggc tcatcaggag
    35761 tgacctcatg ggtggatggc gcgggctttt cactctcgtg cggcgtatcc ggcgagaaat
    35821 ttccagccgg tttgcgggtc gagagctggc gttcaatatc ggtcaggttt tccagcttgc
    35881 gggtggtgag ttccagttgc tgctgtagaa cgtgatgttg ctggcgcaat gtatccagct
    35941 cgctgtcgct ggactgctgg agtttactgt aacgttggcg ctcttccgcc agttgcagtt
    36001 gcagcgcctg accgtcgcgc cagagttgat acagtgggcg aacctgtgcc gggatctcgg
    36061 tacttaacgc ttcaatacgc gcgaccagct ggcggcgctc atacggcgta attttggcgt
    36121 cggcgagcaa aatcccttgt ttaaaggtat tttgccagct gccgtcgtca tattggcgag
    36181 cttgctgacg cgactgcgca ggcattaaac gatcagcaca atccatcgcc cgcagccagt
    36241 aaagcggatt ggtttcggtt gatttacctt gcagcgccca gatgtcgcta cattcagtag
    36301 aaagatagtc agccagttga taaaccggaa ttttttcttc tgctggcgta tcaatggctg
    36361 gcttattgtg attctgcacg caacccagca atgccagaca tggcagccct gccagccaca
    36421 gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt caccagactt aaagcctatc
    36481 ccagtgggcg taattgttgc agacagtctg gacatggaca gcgcggagaa accggggcgt
    36541 acatatcgta cgtgaagatt tcgagcacta cccggggccg aactgacaaa taaaatagcc
    36601 tgataggata ggctgttagc attatttcgt gtttttcgac gacggtaatt caatgcggaa
    36661 acaaacgtct tgcccgctct cgtcgaccag atacagttcc ccttgcatac ggcgaataca
    36721 atccctggca atgcttaatc ccagaccgct gcccttcacc gccccttttc gctggtggct
    36781 tccctgaaaa aagggttcga agatcatggc gcgttcctct tgcggaatgg gcgtgcctgt
    36841 attgatgaca tcaatataaa cccgcgcacc atgtaaactg ctgcgaaggc aaatgttacc
    36901 ggattcagcc ccgtagtgca ccgcattgga gtaaagatta tccagtacgc tcatcagcag
    36961 cattggctcc gccaggcaag ctgttgcttt gagatcgacg tcggtatgca tcattttagc
    37021 ccgtgcgggc aggctatgag cagaaaccac tgtctccacc agcggtgcta attcaacatt
    37081 ctccagttcc accgcactgt ccgcctgttt acggttgtaa tcaagcagtt gttcgatcag
    37141 tttttgcaaa ttgcagctgc tgctatcaag aatgctcacc acctcttttt gctctggcgt
    37201 aagcggcccg acaacctgat cagccagtaa ttcagtgccc tcgcgcatac tcgccagtgg
    37261 cgtttttaat tcatgagata aatgtcttaa aaattgatgg cgttgggatt ccagccatga
    37321 caggcgctca cttaaccaaa gaatacgttg cccaaccgag cgtaactcgc tcggtccaca
    37381 gaacgaaacg ctattgccca gagaacgccc ttcccccaga cggttgatca tgcgctcgat
    37441 atttttcacc ggcccgataa tcatccgcgt gaaaagtagc accatcacca gactcaccag
    37501 aaatagcacc agcgattgcc aaccaaaata ttgcccacgt tcggcgattt cacgctgaag
    37561 ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc accatttcgg tattggcact
    37621 ggcaaaggct tcaagacgcg cggcggcggc agcatcggga ccactgttgt tacactgaag
    37681 ttgggccaga ttgttcaaat cctgacgcaa tgcctggtaa agtttatcgt ctggcagtac
    37741 gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc tggctttgat aaaccttcgc
    37801 aagcgtaggg tcgtccagca cgcaatactg acggtagcta cgctccatct ccagcgccgc
    37861 gttggtcatc gcttcactac gccgggcatc aataagcgta gtgcggttaa ccagcgccgc
    37921 ctgatcgcta agcgcattca ggctttgcca ggcttgccat gccagcacca acaggggtag
    37981 tagaattagc aaaaatgcca gcattaccag ttgtcgtaat gagcggggaa aaacgggcca
    38041 gcgtttcaag gtgttactct cgtcagacgc gaatagcctg atgctaaccg aggggaagtt
    38101 cagatacaac aaagccggga attacccggc tttgttatgg aataaggcgg tgcctaactc
    38161 gacgtttcgc ccgatggttg atatagctac gctgatatca gaagttggac ggcaggcacc
    38221 ttgttgtgcg tcattcgaat tttatgtagc acgtcccgaa ggggctgaca taagtcggtg
    38281 aatgagccac tgcttactat tatgcagtaa ctgtgccaat aaagaaaata gtttggtaac
    38341 gtactgatta tacgttgctt tgagggattt atggcttctt cgctatttga atgtaaatca
    38401 gccactgtgt cgctaaaaag agacaactta agataaactt attagattac aataaaatca
    38461 atcatttaag tgtcgccaga aagcgacacg gtaaaccacc cattgtcgtg atttacagac
    38521 acaaaaaagc tcccggagtt gggagcttat gatagtggtt ggtgcttaat gccgcatccg
    38581 ggctgcaaaa ccaatgaact gatgacttac cccaactgct tacgcgcatt gcggaaaatg
    38641 cgcatccatg ggccatcctc gccccagttt tccggatgcc aggagttgct gacagtacgg
    38701 aaaacacgtt ccgggtgcgg catcataatg gtgactcgac cactttcagt cgtaaccgcc
    38761 gtaataccgt tcggtgaacc gttcgggtta gccgggtagg tttcagtgac tttgccgaag
    38821 ttatcgacat agcgcagtgc caccagccct ttgctttcca gtgccgccag atgcgccgca
    38881 tcacgcactt ccacgcgccc ttcaccatga gagacagcaa tcggcatttg cgagcccacc
    38941 atcccctgca acagcagaga cgggctttgg gttacttcaa ccaggctgaa acgcgcttca
    39001 aagcgatcgg aggtgttacg cacaaaacgt ggccacaact cactccccgg gatcagttca
    39061 cgcaggttag acatcatctg gcaaccgtta cataccccca gggccagcgt ttgcggacga
    39121 tggaagaagg ttgcaaactc atcgcgtaca cggtcattga acaggataga cttcgcccaa
    39181 ccttcaccgg cacccagcac atcaccgtag gagaaacctc cgcacgcgac cagggcgtga
    39241 aaatcctcca gtcccgtacg tccagtcagc aggtcgctca tatgcacgtc gatagcatca
    39301 aagcccccac gttggaaagc tgccgccatt tcaacatgcg agttcacgcc ctgctcacgc
    39361 agtacggcaa ctttcggacg tgcgccagtg gcaatatacg gtgctgccac atcttcgttg
    39421 atatcgaacg acagttttac attcaggccc ggatcggcgt cgttagattt cgcctgatgc
    39481 tcctgatcgg cacactccgg gttgtcacgc aggcgctgca tctgccaggt agtttctgcc
    39541 caccagacac gcaacgtagt gcggctttcg ctgaatacag tctgcccgtt ggcggtaatc
    39601 acaaaacggt caccggaaac cgcctgccca acataatgga cgcaatccgc aagcccatgc
    39661 tgtgccagta cggactcgac cgcttcacgg tcagcggcac gaacctgaat caccgcaccc
    39721 agttcttcgt taaacaacgc cgccaggcga tcgtcaccca gagaggcgat atccgcgtca
    39781 atgccacaat gacctgcaaa ggccatttcc gccagcgtta ccagcaggcc tccatcagag
    39841 cggtcgtgat acgccagcag cttacgctgt gcaaccagcg cctgaatcgc gtcatagaag
    39901 cctttcagtt ggacaacatc gcgtacatct gccggtttgt cgccaagctg acgataaacc
    39961 tgcgccagcg ccgttgcgcc cagcgcgtta ttgcctttgc ccaaatcaat cagcagcagt
    40021 gcgttatctt cggtacaaag ctgcggcgtg atggtgtgac gtacatcttc cacgcgggca
    40081 aatgcagaaa tcaccagcga cagcggcgac gtcatttcgc gctcttcgtt accttcctgc
    40141 cagcgggttt tcatcgacat agagtcttta cccaccggga tcgtcaggcc cagcgccgga
    40201 caaagttctt cacccacggc tttaacggct tcatacaggc ccgcatcttc accagggtgg
    40261 cctgccgccg ccatccagtt ggcggaaagt ttaatgcgtt tgatatcgcc aatctgtgtt
    40321 gcggcaatat tggttaacgc ttcaccgacc gccagacggg cagaggcggc gaaatccagc
    40381 agcgcaaccg gcgcacgctc gccaatcgcc atcgcttcac cgtagtagct gtcgaggctg
    40441 gcggtagtga ccgcgcagtt agcgaccggc acctgccacg gccccaccat ctgatcgcgc
    40501 gccaccatgc cggttacgct gcggtcaccg atggtcacca ggaaagtttt ctccgccacg
    40561 gtcggcagat gcagtacgcg tttcaccgcg tctgcaatgg tgatcccttc acggaccagc
    40621 gcatcgccct tcgctttcag cgtttgtaca tcgcgggtca tcttcggcgt tttaccaagc
    40681 aggacgtcca gcggcagatc gatcggctga ttatcaaaat gacgatcgtg cagagaaaga
    40741 tgcagttctt cggtcgcttc accaatcacc gcgtagggtg cgcgctcacg cttacacagt
    40801 tcgtcaaaca gcggtaattg atcggcagca accgccagca cgtagcgttc ctgggattcg
    40861 ttacaccaga tttccagcgg gctcatgccc ggttcgtcgc ttagaatatc gcgcagttca
    40921 aatttaccgc cgcgtccgcc gtcgctcacc agctccggca tcgcgttaga aagaccgccc
    40981 gcaccgacgt cgtggataaa cagaattggg ttagcatcac caagctgcca gcaacggtcg
    41041 atcacttcct ggcaacgacg ctccatctcc gggttgtcgc gctgtacgga agcaaagtcg
    41101 aggtcggcat cagactgacc agacgccatg gaagacgccg cgccgccgcc aagaccgatg
    41161 ttcattgccg ggccgccgag aacgaccagc ttcgcaccga cgttgatctc gcctttttgt
    41221 acgtgatcgg cgcgaatgtt gccgatcccg cccgccagca tgatcggttt gtgataaccg
    41281 cgcagctctt cgccattgtg gctgttcact ttttcttcat aagtacggaa gtagccgttc
    41341 agtgccggac gaccaaattc gttgttaaac gccgcaccgc ccagcgggcc ttcggtcatg
    41401 atgtccagcg cggtaacaat gcgctcaggc ttaccgaaat cttcttccca cggctgttcg
    41461 aagccaggaa ttcgcaggtt ggatacggag aaaccaacca aaccagcttt cggcttcgcg
    41521 ccgcgcccgg tggcaccttc atcgcggatt tcaccgccgg aaccggtcgc cgcgcccggc
    41581 cacggagaaa tcgccgtcgg gtggttgtga gtttcgactt tcatcaggat gtgcgccggt
    41641 tcctgatgga aattgtagcg gcccgtttcg tggtcagcaa agtagcggcc cacttcagaa
    41701 ccttccatta cggcggcgtt atctttataa gcagagagaa cgtgatctgg cgtggtttcg
    41761 aaagtatttt tgatcatcct gaacagcgat ttcggctgtt gttcaccgtc gataatccag
    41821 tcggcgttaa aaattttgtg gcggcagtgc tcagagttcg cctgagcgaa catatacagc
    41881 tcgatgtcgt tcgggttacg accaagcttt gtgaaagcat cctgcagata gtcaatttca
    41941 tcttccgcca gagccaagcc aagacgcagg ttagcgtcga tcagcgcctg acggccctgc
    42001 cccagcaaat caacgcttgt gaccggagtc ggttgatggt gggcaaacaa ctgctctgca
    42061 tcatctaaat caaaaaagac cgtttccatc atgcggtcgt gcagttcagc ggtaacctgc
    42121 tgccattgtt cattggtcag cgtaccggct tctatatagt aagcaacgcc gcgctcaagg
    42181 cggtttacct gttgtagccc gcagttatgg gcaatatcgg tcgctttcga agaccaggga
    42241 gagatggtgc caggacgcgg ggtcaccagc aggagtttgc cttgcggggc gtggctggcg
    42301 agtgccggac catatttcag caggcgttca agttgtgcgt gctcatcatc gtttaacggc
    42361 gcattgaggt cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg gagcctggca
    42421 gcctgaaaac gtgccagcag tttgttgatt cggaatgccg acagtgcagg cgaaccacgc
    42481 agaatttcca tcataagtct ctcgtcttct aagctttcgg tgtacccaag gggggggaaa
    42541 cgggcgtcat tataaagaat ctgatgcgct gacgaaaccg tttgcgtgga aataaaatca
    42601 ccatcgtgaa ttagcaacgc gtgccgccaa tggctgtaat aagttgccat ctggcgcagg
    42661 tttacgcaaa atgccgctca tttatgagta aacctttcac tattattacg ttttttcaag
    42721 ctgggacgcg cacgacacag agaattaact aattgaaaaa attaaagatt aattatctgt
    42781 tcatcggcat tctggcactg ctgctcgcgg tcgctctctg gccatccatt ccctggtttg
    42841 gtaaagccga caaccgtatc gccgccattc aagcgcgggg agagttgcgt gtgagcacca
    42901 ttcatactcc cctgacttat aacgaaatca acgggaaacc ttttggcctg gattacgaac
    42961 tggcgaaaca gtttgccgat tacctcggcg taaaactgaa agtgaccgtg cggcagaata
    43021 tcagccagct gtttgacgac cttgataatg gtaacgccga cctgctggcg gcaggactgg
    43081 tctataacag tgagcgggta aaaaattatc agcctggccc tacctattat tccgtgtcac
    43141 aacaactggt ttataaagtg ggtcagtatc gcccacgtac gctgggcaac ctgacggcgg
    43201 agcaactcac cgttgcaccg ggtcatgtgg tagttaacga tctccagacc ctgaaagaaa
    43261 caaaattccc ggaattaagc tggaaggtag acgacaaaaa aggctctgcg gaattaatgg
    43321 aagatgtcat cgaaggaaaa ctcgattaca ccattgctga ttctgtcgcc atcagcctgt
    43381 ttcagcgcgt tcacccggag ctcgccgtag cgctcgatat caccgatgaa caaccggtga
    43441 cttggtttag cccgttagat ggcgataata ccctttccgc cgccctgctc gacttcttca
    43501 acgaaatgaa tgaagacggt acgctggcac gcattgaaga gaaatacctg gggcatggcg
    43561 atgattttga ttacgtcgat acgcgcacat ttttacgcgc cgtcgatgcg gtactgccgc
    43621 agttaaagcc cctgtttgag aaatacgccg aagaaattga ctggcgtttg ctggccgcta
    43681 ttgcttatca ggaatcgcac tgggatgcac aggccacttc accgacgggt gtgcgcggca
    43741 tgatgatgtt aaccaaaaat accgcgcaaa gcctcggcat tacggatcgt accgatgccg
    43801 aacagagcat cagcggtggc gtgcgttatt tgcaggatat gatgagtaaa gtgccggaaa
    43861 gtgtgccgga gaacgaacgg atctggtttg ccctcgctgc gtacaatatg ggctatgcgc
    43921 atatgctgga tgcccgcgcc ctgacggcaa aaaccaaagg gaatcctgac agttgggctg
    43981 acgtaaaaca gcgtctgcct ttacttagcc agaaacccta ttacagcaag ctgacttacg
    44041 gctacgctcg tgggcatgaa gcctacgctt atgtcgaaaa tattcgtaag tatcagatta
    44101 gcctggtggg ttatctgcaa gagaaagaga agcaggctac agaagcggcg atgcaactgg
    44161 cgcaggatta tccggcggta tcgcctacgg agttgggcaa agagaaattt ccttttctct
    44221 cgtttctttc ccagtcgtca tcaaactatt tgacccattc tccctctctg ctgttttcca
    44281 ggaaagggag tgaagagaaa caaaattaat ccgtcgagga ttgcgctttt ttctgcgctt
    44341 taatttcctg gcggcgcatg cgaaagaagt cactgagcaa cgccgcgcac tcatccgcca
    44401 gtattccttc cgtaatttcc actcggtgat tcatacccgg atgatgcagc acatccatta
    44461 aagatcccgc agcgccagtt ttcgcgtcac gcgcaccaaa gaccacgcga ccaatgcgac
    44521 tgtggatcat cgctccggca cacattacac atggttcaag cgtgacatac aacgtggcgt
    44581 cgatcagacg ataattttgc atcaccagac caccctgccg cagggccatg atttctgcat
    44641 gtgcggtggg atcatggcga ccaatcgggc ggttccagcc ttcgccgatt acccgattgt
    44701 tatgcactaa taccgcgccg accggcactt cccgctcatc ccaggcacgt ttcgccagcg
    44761 tcagcgcgtg acgcatccaa tattcgtggc taaattcgac ttcagacaaa aagaaaactc
    44821 cggttataaa agcgcggcgc attatacacg gacgctatgc tttactctat tccagttgct
    44881 ggagttcacc gcgcggggtt actcgccaac gatgctgaca gaaataaagc agcggattgt
    44941 cctgattact gtcgctatag ccactgtaca gccgcagcgg cgtgccgatt ttgcgctcca
    45001 gttgtgcgac cttttcatgt cccagacaac gcatcgtcaa tacccaacca ccatagccac
    45061 gctgaatttg gctggcgata agattaaccc gcggcagcca gggcgtatcg aaataaaccg
    45121 cttcaaccag cggctgcgga gagccggtaa tcaaccagat atcagcatcg gaacttaaca
    45181 ggtaggtggt taatcgctcc tgaaccagcg gaaaggcggt aacattgtcg cgaaaccagc
    45241 gcacgaaatc ggcctgcaac gtctgtaaac gtgcttcgct gtgaccaaaa gtgcaccccc
    45301 acagaagcag actcatcggc cagcgtgccg cacgaccttt tatcaataac gcaatggcta
    45361 taatcggtaa caacggcagg acaagtaacg cattcagcgg ttggcgacgc agtaaatagc
    45421 gcagaaaact gccgaacata tcctgctgat gcaatgttcc atctaagtca aaaaacacca
    45481 cacgacgctc gtgagttgcc aaaccatact cctctggact gaaacatcct gattaatttt
    45541 cgctactaca tagcctaaca gatagatcat cacttttccg gcaacaatcg accgcatcat
    45601 ggctaactgg aatttttaat tcatggcaat tagcggcaat ggaatataaa attcactcgc
    45661 gtgtgtctca tattgcctgt tgtcgccact atttcatcgc gaggaaagat atgaacggct
    45721 tacttcgtat ccgtcagcgt taccaagggc ttgcccagag tgataaaaaa ctggcggatt
    45781 atctgctgct acaacctgat acggcgcgcc atttaagctc tcagcaactg gccaacgaag
    45841 ccggagtcag tcagtccagc gtcgtgaagt tcgcgcaaaa actcggctat aaaggttttc
    45901 cggcgcttaa gttggcgttg agtgaagcgc tggcaagcca gccggaatcg ccctccgtgc
    45961 ccattcataa ccaaatccgc ggtgatgatc cgttacggct ggtcggcgaa aaactgatta
    46021 aagaaaatac cgccgctatg tacgcaacgc taaacgttaa tagtgaagag aaactgcatg
    46081 aatgcgtaac aatgttgcgc tctgcgcggc ggataattct gaccggtatt ggcgcttcgg
    46141 gtctggtggc gcaaaacttt gcctggaagc tgatgaagat tggcttcaat gctgccgcac
    46201 tgcgcgatat gcatgcgcta ctcgcaacag tacaggcgtc gtcccctgac gatctgttat
    46261 tagccatttc ctacaccggt gtacgacgcg agttaaacct ggcggcagat gagatgctgc
    46321 gagtgggcgg aaaagtgctg gcgattaccg gctttactcc gaatgccctg caacagcgtg
    46381 cttctcattg cctgtatacc attgccgaag aacaagcgac aaacagtgct tcaatctctg
    46441 cttgtcacgc tcagggaatg ttaacggatt tgctgttcat tgcgctgatt cagcaggatc
    46501 tggaactggc accagaacgt attcgtcata gtgaagcgct ggtgaaaaaa ctggtctgag
    46561 taaagaatgc gcgtataatg cccgcccggt ttgtgttgtt ttgagagttt ccttatggcg
    46621 ctgttaatca ctaaaaaatg catcaattgt gatatgtgtg aaatcaaata aaaacacatt
    46681 tattttcata gtgatacagc aatgtgttat tgaatgtgtt gttggattta tggtgcaaaa
    46741 aaattttgca taagttcagc aacacaaaga acaccacttt ctttaacgtc ctccgcgccc
    46801 taaaggacgg ggctttacgg cgcaaaaggc ctgtgacata caacattaac ttttcagtca
    46861 accgtagcac gttcttgtat acgccgtgtc tgcggtttta tataaccctg gctggaaacc
    46921 tcttatacaa agtcgattcg ccaacatcat agatgatcgc caccttctgg cgagaaactc
    46981 ctgatgcaat tagtcgcccg gcctgcgccc attattctgg tgaaagtttg ggacggcgac
    47041 cgccaattcg tccctgtgcg cgagcagctt ccagtcctgc ttttgttcgt tcaacaatca
    47101 gttcacgttc catttcagcc agggcaccca tcacatggaa gaaaaagcgt cccattggtg
    47161 tgctggtatc aattgaatca gtcagactac gaaagttgat gcctcgttcg cgcaacgcct
    47221 ccaccagcac gacaagatgc cgcatactgc gccccagccg atccagcttc cagacaacca
    47281 gtgtgtcgcc tgccgataat gtcttgagca gttttttcag ccccggtctt tcggactttg
    47341 taccacttat cttgtcttca aaaatcagct cgcatcctgc acagtt
//
LOCUS       DAJTUM010000023        45777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTUM010000023 DAJTUM010000000
VERSION     DAJTUM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            114..2267
                     /locus_tag="OO471_001379"
     CDS             114..2267
                     /locus_tag="OO471_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6242705.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO471_001380"
     CDS             2320..4050
                     /locus_tag="OO471_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242706.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO471_001381"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO471_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6242707.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO471_001382"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO471_001382"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6242708.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO471_001383"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO471_001383"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6242709.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO471_001384"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO471_001384"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6242710.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO471_001385"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO471_001385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6242711.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO471_001386"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO471_001386"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6242712.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO471_001387"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO471_001387"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6242713.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO471_001388"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO471_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6242714.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO471_001389"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO471_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6242715.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO471_001390"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO471_001390"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6242716.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO471_001391"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO471_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6242717.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO471_001392"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO471_001392"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6242718.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO471_001393"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO471_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6242719.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO471_001394"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO471_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6242720.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO471_001395"
     CDS             16308..16847
                     /locus_tag="OO471_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6242721.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO471_001396"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO471_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6242722.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO471_001397"
     CDS             complement(18206..18553)
                     /locus_tag="OO471_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6242723.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO471_001398"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO471_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6242724.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO471_001399"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO471_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6242725.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO471_001400"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO471_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6242726.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO471_001401"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO471_001401"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6242727.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO471_001402"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO471_001402"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6242728.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO471_001403"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO471_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO471_001404"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO471_001404"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6242729.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO471_001405"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO471_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6242730.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO471_001406"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO471_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6242731.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO471_001407"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO471_001407"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6242732.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO471_001408"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO471_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6242733.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO471_001409"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO471_001409"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6242734.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO471_001410"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO471_001410"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6242735.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO471_001411"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO471_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6242736.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO471_001412"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO471_001412"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6242737.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO471_001413"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO471_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6242738.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO471_001414"
     CDS             complement(40011..40466)
                     /locus_tag="OO471_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242739.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO471_001415"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO471_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO471_001416"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO471_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6242740.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO471_001417"
     CDS             complement(41309..41986)
                     /locus_tag="OO471_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6242741.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO471_001418"
     CDS             complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO471_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6242742.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO471_001419"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO471_001419"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6242743.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO471_001420"
     CDS             complement(43192..43650)
                     /locus_tag="OO471_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6242744.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO471_001421"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO471_001421"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6242745.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO471_001422"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO471_001422"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6242746.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11902 a        11938 c        11201 g        10736 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atccactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttataat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgagcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gataccaggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaaaccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtt gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagacct ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcaacaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg tgcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactct ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagc
//
LOCUS       DAJTUM010000024        42787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTUM010000024 DAJTUM010000000
VERSION     DAJTUM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO471_001423"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO471_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6242747.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO471_001424"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO471_001424"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6242748.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO471_001425"
     CDS             complement(3736..4092)
                     /locus_tag="OO471_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6242749.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO471_001426"
     CDS             complement(4096..4512)
                     /locus_tag="OO471_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6242750.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO471_001427"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO471_001427"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6242751.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO471_001428"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO471_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO471_001429"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO471_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6242752.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO471_001430"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO471_001430"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6242753.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO471_001431"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO471_001431"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6242754.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO471_001432"
     CDS             10521..11504
                     /locus_tag="OO471_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6242755.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO471_001433"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO471_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6242756.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO471_001434"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO471_001434"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6242757.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO471_001435"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO471_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO471_001436"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO471_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6242758.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO471_001437"
     CDS             complement(15306..15515)
                     /locus_tag="OO471_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6242759.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO471_001438"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO471_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6242760.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO471_001439"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO471_001439"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6242761.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO471_001440"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO471_001440"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6242762.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO471_001441"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO471_001441"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6242763.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO471_001442"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO471_001442"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6242764.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO471_001443"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO471_001443"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6242765.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO471_001444"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO471_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO471_001445"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO471_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6242766.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO471_001446"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO471_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6242767.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO471_001447"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO471_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6242768.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO471_001448"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO471_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6242769.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO471_001449"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO471_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6242770.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO471_001450"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO471_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6242771.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO471_001451"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO471_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6242772.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO471_001452"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO471_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6242773.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO471_001453"
     CDS             complement(34461..34604)
                     /locus_tag="OO471_001453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242774.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO471_001454"
     CDS             34605..34883
                     /locus_tag="OO471_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242775.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO471_001455"
     CDS             complement(34930..37026)
                     /locus_tag="OO471_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6242776.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO471_001456"
     CDS             complement(37026..37763)
                     /locus_tag="OO471_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6242777.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO471_001457"
     CDS             complement(37760..38398)
                     /locus_tag="OO471_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6242778.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO471_001458"
     CDS             complement(38512..38754)
                     /locus_tag="OO471_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242779.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO471_001459"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO471_001459"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6242780.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="OO471_001460"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="OO471_001460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6242781.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10642 a        11013 c        10502 g        10630 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgataccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttctt
//
LOCUS       DAJTUM010000025        40546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTUM010000025 DAJTUM010000000
VERSION     DAJTUM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..956)
                     /locus_tag="OO471_001461"
     CDS             complement(<1..956)
                     /locus_tag="OO471_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6242782.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO471_001462"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO471_001462"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6242783.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO471_001463"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO471_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO471_001464"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO471_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6242784.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO471_001465"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO471_001465"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6242785.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO471_001466"
     CDS             complement(5018..6346)
                     /locus_tag="OO471_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6242786.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="OO471_001467"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="OO471_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO471_001468"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO471_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6242787.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO471_001469"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO471_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6242788.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO471_001470"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO471_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6242789.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO471_001471"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO471_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6242790.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO471_001472"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO471_001472"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6242791.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="OO471_001473"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="OO471_001473"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6242792.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="OO471_001474"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="OO471_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6242793.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO471_001475"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO471_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6242794.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="OO471_001476"
     CDS             18267..19166
                     /locus_tag="OO471_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6242795.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="OO471_001477"
     CDS             19172..21604
                     /locus_tag="OO471_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6242796.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="OO471_001478"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="OO471_001478"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6242797.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="OO471_001479"
     CDS             22717..23982
                     /locus_tag="OO471_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6242798.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="OO471_001480"
     CDS             complement(24223..25815)
                     /locus_tag="OO471_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6242799.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="OO471_001481"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="OO471_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6242800.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="OO471_001482"
     CDS             complement(27012..28130)
                     /locus_tag="OO471_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6242801.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO471_001483"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO471_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6242802.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="OO471_001484"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="OO471_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6242803.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="OO471_001485"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="OO471_001485"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6242804.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO471_001486"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO471_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6242805.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="OO471_001487"
     CDS             complement(31379..31444)
                     /locus_tag="OO471_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242806.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="OO471_001488"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="OO471_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6242807.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="OO471_001489"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="OO471_001489"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6242808.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="OO471_001490"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="OO471_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6242809.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="OO471_001491"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="OO471_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6242810.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="OO471_001492"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="OO471_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6242811.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="OO471_001493"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="OO471_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO471_001494"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO471_001494"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6242812.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="OO471_001495"
     CDS             39719..39979
                     /locus_tag="OO471_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6242813.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40546
                     /locus_tag="OO471_001496"
     CDS             40244..>40546
                     /locus_tag="OO471_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6242814.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10096 a        10719 c         9868 g         9863 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaatg tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcgtg
//
LOCUS       DAJTUM010000026        38789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTUM010000026 DAJTUM010000000
VERSION     DAJTUM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO471_001497"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO471_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6242815.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            531..572
                     /gene="hisL"
                     /locus_tag="OO471_001498"
     CDS             531..572
                     /gene="hisL"
                     /locus_tag="OO471_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6242816.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="OO471_001499"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="OO471_001499"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6242817.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="OO471_001500"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="OO471_001500"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6242818.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="OO471_001501"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="OO471_001501"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6242819.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="OO471_001502"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="OO471_001502"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6242820.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="OO471_001503"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="OO471_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6242821.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="OO471_001504"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="OO471_001504"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6242822.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="OO471_001505"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="OO471_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6242823.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /locus_tag="OO471_001506"
     CDS             7140..7751
                     /locus_tag="OO471_001506"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6242824.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO471_001507"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO471_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6242825.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO471_001508"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO471_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO471_001509"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO471_001509"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6242826.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12304)
                     /locus_tag="OO471_001510"
     CDS             complement(11945..12304)
                     /locus_tag="OO471_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242827.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12954..13517)
                     /locus_tag="OO471_001511"
     CDS             complement(12954..13517)
                     /locus_tag="OO471_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242828.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13724..14662)
                     /locus_tag="OO471_001512"
                     /pseudo
     CDS             complement(13724..14662)
                     /locus_tag="OO471_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14725..15099)
                     /locus_tag="OO471_001513"
     CDS             complement(14725..15099)
                     /locus_tag="OO471_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6242829.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15103..16251)
                     /gene="wzy"
                     /locus_tag="OO471_001514"
     CDS             complement(15103..16251)
                     /gene="wzy"
                     /locus_tag="OO471_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6242830.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16271..17176)
                     /gene="rfbG"
                     /locus_tag="OO471_001515"
     CDS             complement(16271..17176)
                     /gene="rfbG"
                     /locus_tag="OO471_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6242831.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17169..18059)
                     /locus_tag="OO471_001516"
     CDS             complement(17169..18059)
                     /locus_tag="OO471_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6242832.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18089..19345)
                     /gene="wzx"
                     /locus_tag="OO471_001517"
     CDS             complement(18089..19345)
                     /gene="wzx"
                     /locus_tag="OO471_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6242833.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19361..19906)
                     /gene="rfbC"
                     /locus_tag="OO471_001518"
     CDS             complement(19361..19906)
                     /gene="rfbC"
                     /locus_tag="OO471_001518"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6242834.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19911..20789)
                     /gene="rfbA"
                     /locus_tag="OO471_001519"
     CDS             complement(19911..20789)
                     /gene="rfbA"
                     /locus_tag="OO471_001519"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6242835.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20848..21747)
                     /gene="rfbD"
                     /locus_tag="OO471_001520"
     CDS             complement(20848..21747)
                     /gene="rfbD"
                     /locus_tag="OO471_001520"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6242836.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21747..22832)
                     /gene="rfbB"
                     /locus_tag="OO471_001521"
     CDS             complement(21747..22832)
                     /gene="rfbB"
                     /locus_tag="OO471_001521"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6242837.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23205..24098)
                     /gene="galF"
                     /locus_tag="OO471_001522"
     CDS             complement(23205..24098)
                     /gene="galF"
                     /locus_tag="OO471_001522"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6242838.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24273..25667)
                     /gene="wcaM"
                     /locus_tag="OO471_001523"
     CDS             complement(24273..25667)
                     /gene="wcaM"
                     /locus_tag="OO471_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6242839.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25678..26898)
                     /gene="wcaL"
                     /locus_tag="OO471_001524"
     CDS             complement(25678..26898)
                     /gene="wcaL"
                     /locus_tag="OO471_001524"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6242840.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26895..28175)
                     /gene="wcaK"
                     /locus_tag="OO471_001525"
     CDS             complement(26895..28175)
                     /gene="wcaK"
                     /locus_tag="OO471_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6242841.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28355..29833)
                     /gene="wzxC"
                     /locus_tag="OO471_001526"
     CDS             complement(28355..29833)
                     /gene="wzxC"
                     /locus_tag="OO471_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6242842.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29835..31229)
                     /gene="wcaJ"
                     /locus_tag="OO471_001527"
     CDS             complement(29835..31229)
                     /gene="wcaJ"
                     /locus_tag="OO471_001527"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6242843.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31284..32654)
                     /gene="cpsG"
                     /locus_tag="OO471_001528"
                     /gene_synonym="manB"
     CDS             complement(31284..32654)
                     /gene="cpsG"
                     /locus_tag="OO471_001528"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6242844.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32847..34283)
                     /gene="cpsB"
                     /locus_tag="OO471_001529"
     CDS             complement(32847..34283)
                     /gene="cpsB"
                     /locus_tag="OO471_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6242845.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34286..35509)
                     /gene="wcaI"
                     /locus_tag="OO471_001530"
     CDS             complement(34286..35509)
                     /gene="wcaI"
                     /locus_tag="OO471_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6242846.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35506..35985)
                     /gene="gmm"
                     /locus_tag="OO471_001531"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35506..35985)
                     /gene="gmm"
                     /locus_tag="OO471_001531"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6242847.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35985..36953)
                     /gene="fcl"
                     /locus_tag="OO471_001532"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35985..36953)
                     /gene="fcl"
                     /locus_tag="OO471_001532"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6242848.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36956..38077)
                     /gene="gmd"
                     /locus_tag="OO471_001533"
     CDS             complement(36956..38077)
                     /gene="gmd"
                     /locus_tag="OO471_001533"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6242849.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38103..38651)
                     /gene="wcaF"
                     /locus_tag="OO471_001534"
     CDS             complement(38103..38651)
                     /gene="wcaF"
                     /locus_tag="OO471_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6242850.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38667..>38789)
                     /locus_tag="OO471_001535"
     CDS             complement(38667..>38789)
                     /locus_tag="OO471_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6242851.1"
                     /translation="CADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10355 a         9714 c         8730 g         9990 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaaa
    12361 taatacacac aaggataaaa tatcgtccca acttgtaatt tgtaatgttg atatctttta
    12421 ttaaacttgc aattgagatc gcaacctgca atactgacaa tgggtaaatg ggtgataaat
    12481 acctgtcacc ttgcgtaaca gaaaaccaaa ataagacata tgttaaaaca atcgagaaaa
    12541 atacccaaag tattttatta ttcctaagtg taaagacaga cagtatagca taaaaaacaa
    12601 catcactgcc aactttatta attgcttgtt gcaaatctaa tgtggcagga cttataccat
    12661 gtcgagcttg ttctgctgtt tgaacttgta gatcaatttc attccatagg tagtgaccaa
    12721 atatatttcc tccagcagga ctaaaaggat caccagagat ccaaaaacta cggacatacc
    12781 aataaatgcc accaagaatt gttgccaagc taaaaataag aaatgtctgt atgtttctct
    12841 ttttcgctat caatataaca aatatgatgg ctccatatat ggcaccaaat agttttacgc
    12901 cacaagcaat tccaataaaa aaaccacata aaaaaattaa ttcattttgt gttttattgt
    12961 tttctgagcg aatatatata agaatactgg ctgcagcaaa acaaagatag cgttaaatca
    13021 atatatgcat aaccaagatg atctttaagt ggattgatag taaatagtag gattgtagca
    13081 ataatgcctg gataataagt tttcgtaatt tcccatgaac agccaacaag tccacaggct
    13141 attacaaaaa aacagatatt tgctaaaaac tgagctaacc ttacatctcc taactgtaaa
    13201 cctaaagcca ttaaaagatc tgcattttgc gggaacagag gaaaacgcaa atattgttca
    13261 acaacaattg catggtgctc aactaatgaa cgagccaaag gtaagtgata catagtatca
    13321 tcccaatcac cgggagctcg cagggacgat aacaatgtaa ccacaaaaat caaaacaaga
    13381 aataaaataa caaacagcgt actgaactca atattattta ttgccagcca tctaaattgc
    13441 tgacgtttaa taatgcttaa aacccaacac accgtaaaaa ttaaaaagat agcaatctta
    13501 ttaaattgcc cgaccatgcc cagaaagtaa attatcaaaa tatttaaaga catccctatg
    13561 cataggcaat aaaaaataaa gaaaatttgt taagtcctgc caccccctcc ttaaaaaaag
    13621 atgacaatac aatattacca atgacaaaaa atgtcattcc aaaagcaaaa caatacacaa
    13681 catacaaata caacggcaat tcaatcatag taaaatactc tatttacatt atcagtttat
    13741 agtttttttc agaaagataa aataatgcct tcatttcacg tctagttgtt gtatttgaat
    13801 gcaaaataat gccacataca aatgccatta ttgacgataa catgatggag gcagttaaaa
    13861 aggcagtcgg aaatcttctc accaaacctg tatcaatata atcaacaatg atgggcaatc
    13921 ctaaaattat agacaaagca gcaagtaaaa cgccgataat actaaaaaag tatagtggcc
    13981 gttcaagcga gtaaagttta attattgttt tcagaatctt gaacccatca ctccaagttg
    14041 atagtttgct cacactccct tcagatcgct cgccatattt tgtattaacc tcaccatact
    14101 tcatacgtaa ttctaaagca tgaattgtaa gctcagtttc gatttcaaat ccccttgata
    14161 gacatggaaa agatttaaca tagcgtctgg aaaatacacg ataccccgac aacatatcag
    14221 taaaattccc tttaaagata gtctgaacag taccagtcaa caatttattt cctcagcgat
    14281 gcccttttct gtatgtattt tgatctccat tctcttgacg acatcctaca accatatcca
    14341 attgattttt tatgagatgg ttaatcattt ctggagcaga acttgcatca taagtgtcat
    14401 ctccatcaac cattagataa atatccgcat ctacatctga gaacatacgt cttacgacat
    14461 tccccttgcc tttcagagga acagagtgaa ctgttgctcc agcatcctct gccacttgtg
    14521 ctgtactgtc agtcgaatta ttatcaaata catatataga agcagttgga atattcgttt
    14581 tgaaatcatt tatgactttt gctattgcct tctgctcatt ataacaagga ataattacag
    14641 cgatattaaa agagtgatac ataagcccct catataaact atttagctaa tgtgcaagta
    14701 attgtgtttt taacaaatcg tttactaacg aaaaatgaaa tattttgatg cgcaatatcc
    14761 taaaaccaaa ctaaaaagag aaaaggtgaa caatgtaaat attggagata aagctaagag
    14821 atcaaataaa acgccgaata aataactcat cgcccccata aaaaatataa atataaaata
    14881 tctagccttg gttgcagata ctttaaatga gcatcttgca tttgcaaaaa aagaaaaggt
    14941 tgctgcacat ataaaaccaa caatgttact tcggccttga ccaaaagcta aattattata
    15001 acacgcataa aatacccccc aatgtaaagc agtattgaca attccaatta aaaaataact
    15061 aaaaaaagaa ctggtcaata acttccctat ttttaacatc ctttattttg ctccagaagt
    15121 gaggttatta ctaatttgga tattttctat agaaaatacc cataaaacta caacgcctat
    15181 aataaaaatt cccatgggtg taaaaagaaa tggattagtt atagaaacgg taatactgaa
    15241 aaaaagtatt aacgacatca ttaatgatgt ttttgtacta tttaaaaaat agcctttcaa
    15301 aaagataagc aacataggaa ctaatgatat aaatacacca ataacgcctg ttttctgaag
    15361 tatctcaaga ggtactattt caatccttaa tcgatctaat ataaatgaac caaatcctct
    15421 tccaaacaag aacgtcgcta aatcaacatt tttataataa aaatataaat cattaaatct
    15481 aacagagtca gaatcttcac tgcgtgatcc gaccatgtaa agcataatta taaatagaac
    15541 gctgcccaat attattataa tagtatttaa atatagctta tgtaatttaa aactggctaa
    15601 taacagtgat aatgttgtaa aaagtaatac acctcttgat tctgtcaata atatcgcaag
    15661 cactattatg agacatttta aatatttttt gtcgacaaaa ctgataatcg caccgacacc
    15721 aaaaaagtag aaggatttgg aaaaaaaagc cccatcggga cgaaaaaaga actcgctagt
    15781 caatgataag tgttcataaa ttaaattaaa atttaacaaa ccgaaattca acaaaattat
    15841 gtatgaaata tataacagtg atccatataa tgcaaatgca ttaactattt taataaaaat
    15901 ctcacatgaa aatactttta aagtatatct tagtgtaaat agatatataa gtgacagcat
    15961 aaaaaggtat ggttgcacat caaacagtat atcatttatt ttattaccag ctaataagcc
    16021 gacaaagaag ccataaaaag gaaataaaag caaccatata aaaagactga cacatttttg
    16081 ggtgattgaa aaattgaatg taactaaatt aaaaattaaa agtataaaac taaatacaaa
    16141 taataaatat cgcaaaggga atattcccaa tggctccagt aatcttccac caccaccaat
    16201 aatcagttca atacataaaa atgttataaa aattttattt atattattca tattcgtaag
    16261 gtgatgtttt ttaaaatatg tttaaaatca acattaataa aatataaatt ttatcagcct
    16321 ttttccgcct taacttccaa aaacttaaag ccaagatcaa attgcctata taacctttac
    16381 ccaattgatt attcacaaag ctatcatcag ctaaggcatt agctatttta gttacttctt
    16441 ttcgatacca cttgtttctc accaaaccaa gccgtttgta ttttgcttta aaactaatgt
    16501 ttgctcccac ttggttgttt tcatgctgtc tgtacagcat tgttggtttt ctatcaatat
    16561 accacgaata atttctcgtc cgcgcaaatg aatataaaaa ccaatcatgc agacagatat
    16621 cttgtgcagc atttttatta tttatgatga acttcttaaa ttcaataagc gtttcttttt
    16681 ttataacata tgtacacccc ggacctgctg cttcaaaaaa ataatcgaat tgagtttgcg
    16741 actgagcctt atcaactaaa ttttttcgcc ctgatggata atatgcaatt acattgctgg
    16801 aataacaatt cgcattatta aataccaagc agtcaattgc attttttatt ttatctttat
    16861 accaaatatc gtcctgatca ctaaaacaga tataatcata gttttcaaga tcgacatctt
    16921 taattaatct ataaaaattt ttacctgccc ccccaaactt attgacgctt ggaagtatat
    16981 ttataattga gggatatgat aattgaaatt cttcacaaat atttaatgta ttatcagttg
    17041 ataaatcatc tgatataaaa attgagatgt ctacatcttt ttgttcaata atacttttta
    17101 attgttcctc aatccagcat tcgccattat acgtagccat aagtacagct actctttttt
    17161 gatcagaact atgcattata acgaccgccc ccagtaattc ctcttattcc atccagcgta
    17221 gccaaaatat gttttttcat atattcaatt ttattttttt gagttgcgat aattaaagtc
    17281 gtatgaatta aagaaaaaac aacttcccta atagagagta aaagaggcac atgatttttt
    17341 ctaagcaaaa gaaatgcatt tcttactcga taataacgac gtactggcga gtgaattggt
    17401 atgttaatcc cacagatttt tactgactta tccccaatag catgaaccat ttctatatca
    17461 ggaatgatat gaactaatat gccatagttt aagcatctaa gacaccattc tgtatcgaca
    17521 taatcaataa aaagttcctc atccatcatt ccaacaatct caaaaacttc ttttgaaacc
    17581 atggttccgg atgagattgt tactgaactt ttaaacggtg tctgtccttc agtgacatga
    17641 attttctctc gaagaccatt ttttttgata ttacaaatag gataataatg tgacttatct
    17701 ctatcaataa aaactgggcc agctattttt gcattttcat tatccatata gcaggaaagt
    17761 tttttgataa actcattgcg aatacggcta tcttgatcaa aaaaaattat cttctctgcc
    17821 cctttttcaa gagaatatcg aatcccaata ttttgtgctt ttgctatacc ttcattccta
    17881 ccaagacaaa ttaaatgaac tttttcaatg tctgctaatt tatctttcaa ggtgaaatta
    17941 gtcctattat cgactactac aacgtaacaa ttttgctcct ttagttcagt gatcagtgca
    18001 ttcagatttt taagttcggg attatatgtc acaatgacag cgtaaatatt actattcatt
    18061 atccaagtga ctcagtaatt ggttaatttt agttttcata tacagaacac attctagtat
    18121 aaagccattg taataacaga gcatctaaaa ttacacgtat gctccaggat attgcggcac
    18181 ccagtaaccc atatttttcg accataaaat acaaaagggc taaataagga accaattcag
    18241 cacaatgaat caaagcagta attttagatt ttcctaatgc ctggattgca gagaatggta
    18301 tcattgccag cgcattgaag aaaaaaccga tcaataacac atttaatata ttaaaggaat
    18361 taatttgata attctcacca aaccatattt tcaatacaag ccctgaatat aacaagccta
    18421 tgattatcac cggcaaacag attagaaaca ttaaaagcaa tgatttattt acattacgtt
    18481 taaattcttt aaagtctttg atgttactta accttggaaa cactgccctc ccgattgctg
    18541 caggtattat tcctaatttt agaataacct ccgctggtgc agaataaaat gcaactttat
    18601 ctgcccccat aatatttgac actataaatc gatcaaaata caccatgacc ggacttatga
    18661 tattacttac tgtcatccaa ccgccaaaga aaaagagtcg tttaaaggtt ttataacaaa
    18721 aatgaacccc cgaaatttta atatcattcc tgacataata ggcagagact aaaatcgaaa
    18781 taactcttgc aaatattaaa ccagcaaccg ctgccgacaa tgtagcacta taaaaaacaa
    18841 atatggccgg aattcccgcg atgcaagagc ttgatataga tttttgaata tttacaatgc
    18901 caaatttttc atccccctca agaatggctg accataattg atttagaata aataatggaa
    18961 tgcaaatagc caacagttta aatgctagtt gtatatcact atgttcaaca ccagaaattt
    19021 ttagataatt aacaattcca tcagagaaaa tcaataataa aaaagcacca aagcatgaaa
    19081 atagcaccaa aaaagatgta cttgttgaaa ttaccttggt tctttcatga tgattatcac
    19141 gatgaattgc aatttctcta ataacagagc gagtcaggcc tacatcaaaa attccagcat
    19201 acccaacaag tgcaattgca attgtataaa caccgaaacc ttcaggcccc aaacctcgag
    19261 cgagaaatcc tagcgagggg atagcaatta aagttggtat tgcatagcca aaaaggttcc
    19321 agacactatt ttttattatg ctcattatta catttcttta tcatataaaa taatcagcat
    19381 ctacaaattt ttgaccatta atatctttat cagaaagcaa tggattttga accggccatt
    19441 ctatatttat acttttgtcg ctccataaaa tcccacgctc ttgcattttt gaatagaaat
    19501 tatttgtttt gtagacaaat tctgcttcat cactaagaac gcaaaaccca tgagcgaatc
    19561 cttccggtat ccataattga cgcttattct ccgccgacaa atttacacca acccacttgc
    19621 caaatgttgg tgatgatttc cgaatatcaa cagctacgtc aaaaacttca ccgacaacac
    19681 agcgtaccag ttttccttga gcataaggtt ccagctgata gtgcagtccg cgtaacacac
    19741 ccttagttga tttagaatgg ttatcctgaa caaactcaac ctttcgtcct acagcctctt
    19801 cgaaaacttt ctgattgaag ctttccataa aaaagccccg ctcatcacca aatacttttg
    19861 gttcaaaaat tagcacatca ggaatttcag ttttaattac gttcatttta ttaataacct
    19921 ttaatcattt tcagcagata ctgaccataa gcatttttct tcagcggctc ggctaatacc
    19981 ttcacttgct cagcatcaat aaagccttta cggtgagcaa tttcttccgg gcaggaaact
    20041 ttcagcccct gacgctcttc aattgttgca atgaagttgc ttgcttcaat aagactttga
    20101 tgtgtccccg tgtccagcca tgcataacca cgccccatca tagcgacaga caaacgtcct
    20161 tggtccatat aaatacggtt aatatcggta atttccagtt cgccacgtgc agatggttta
    20221 aggtttttcg ccatttctac aacgtcattg tcatagaaat aaagcccagt aaccgcatag
    20281 ttactttttg gttcctgcgg tttttcttcc agactaattg ccgtaccgtt atcatcaaat
    20341 tccaccacac cgtagcgctc aggatcattt acgtgatagg caaaaaccgt tgcgccactc
    20401 tctctgttaa cagcggtatc cattaatttc ggcaggtcgt gaccatagaa gatattatca
    20461 ccaagtacca gcgcacaatc atcaccacct ataaattctt caccaatgat aaatgcttgc
    20521 gccagaccat ccggactcgg ttgtacttta tattgcagat taagccccca ctggcttcca
    20581 tcaccaagca attgttgaaa acgtggtgta tcctgcggcg tactgataat cagaatatct
    20641 cgaatacccg ccaacataag cgttgaaagc ggataataaa tcatcggttt atcataaatc
    20701 ggtagcagtt gtttgctcac agccattgtc acaggataaa gacgagtgcc ggaaccacca
    20761 gccagaataa tacctttacg cgttttcatt tcaccattcc ttttaattca tctcgctcta
    20821 gcatcatgag cgagatgcaa taatttgtta aattgccgtg gtcgtaaata attcgttgag
    20881 catacgtttc acgcccacct gccagtcagg caagacaagc gcaaagttct gctgaaattt
    20941 ttcggtattg aggcgagaat tatggggacg acgtgctggt gtgggatagg ccgttgtcgg
    21001 cacggcatta agtttgttaa gagcaaggtt gatccccgct ctgcgcgctt cttcaaatac
    21061 cagagcggca taatcgtgcc aggttgttgt accaccagca accagatggt acaaaccagc
    21121 aacttctggt ttatttgcgg ccacgcgaat ggcatgagcg gtgcaatcag ccagtaattc
    21181 agcccccgtt ggtgcgccga attgatcgtt tattacagcc aactcttcgc gttcttttgc
    21241 caggcgcaac atcgttttgg caaagttatt tcctttacca gcgtataccc agctggtacg
    21301 gaaaataaga tgctttgcgc aatgttcttg taacgctttt tccccagcta gcttggtttc
    21361 accgtagaca tttagcggtg ctgttgcatc cgtttccagc catggcgtgt caccgttgcc
    21421 tgggaaaaca taatcagtgg agtagtgtat aacccaggct ccgacttcat tagctgcttt
    21481 tgcaatcgct tcgacgcatg tcgcgttaag taattgcgcg aaattcggtt ctgattccgc
    21541 tttgtctact gcagtgtgag cagcagcatt aacaataaca tcggggcgaa tttttttgac
    21601 ggtttcagcc acaccttcgg ggttgctgaa atctccacaa taatcagtgg agtgaacatc
    21661 gagcgcaatc aagttaccca gaggagccag agcacgctgc agttcccaac ccacctgccc
    21721 tgttttgccg aaaagcagga tattcattac tgacggccct catagttttg ttcaatccac
    21781 gactgataag taccgctttt cacattctca acccaatttg tattagccag ataccattcc
    21841 accgttttac gaatcccgct ctcaaacgtt tcttgcggtt tccagcccaa ttcgcggcta
    21901 attttatcgg catcaatggc gtaacggcgg tcatgccccg ggcgatcggc aacataagta
    21961 atttgctcac gataagattt ctctttcggt acaatctcat ccaacaaatc acaaatagtg
    22021 agcactacgt cgatgttttt cttttcgtta tgtccaccaa tgttataagt ttcacccgct
    22081 ttaccttcgg ttacgacggt atataacgca cgagcatgat cctctacata caaccagtcg
    22141 cgaatttgat cccctttacc ataaataggt aatgccttac cttccagagc attcagaata
    22201 accaatggaa taagtttttc cgggaagtga taaggaccat aattgttcga gcaattagtc
    22261 acaatggttg gtaaaccata agtccgcttc caggcacgta ccaaatgatc gctggaagct
    22321 ttagacgcag aatacgggct acttggcgcg taagctgtcg tttccgtaaa tagcggtaac
    22381 gcttcgttat tatttgcttc atccggatgg ggtaagtcac cataaacttc atcagtagaa
    22441 atatgatgaa agcggaaact ttttttcttt tcatcattca gagcagacca gtaattgcgc
    22501 gccgcttcta aaagaacata agtccccaca atattggttt caataaatgc cgcagggccc
    22561 gttattgaac ggtcaacgtg gctctctgcc gccaggtgca tcaccgcgtc tggctggtgc
    22621 tgtgcgaaaa tacgcgacat ggctacagca tcgcagatat ctgcatgctc aaaagcataa
    22681 cgttcagaat cagaaacatc agcgagtgat tccaggttcc cggcgtatgt taatttatcg
    22741 acattaacaa cactatcttg cgtattattt attatgtgac gaacaacagc agaaccaata
    22801 aaccctgcgc caccagtaac aagtatcttc acttttttat tccatatagc tatagagcat
    22861 gctgtgaaat agactgctcc agatttgatt aatagaggta ttaatacacg ctaccgcccc
    22921 tggcttaaca gctaccagtg cactgcatgc atctcaacga tgtggcgagc gttagccact
    22981 cgcgccaaat ccgaaaaatt caaacgttaa ttgtcttacc aaacagccgt agaaacaagg
    23041 aaaatcctgg aaaaatttga ataatatgag ctatgtaact tcttgttttt ataggcttta
    23101 taaaaacggc aacggcagga aaattcaatg agcaaaactt atgccacgaa tcttcactgc
    23161 cgttataatt ttcttatcaa ccgttacatc cggtcagatt ttcattattc gcttaacagc
    23221 ttctcaatac ctttacggaa cttcgcccct tctttcaggt tgcgtaggcc atacttcaca
    23281 aacgcctgca tatagcccat ttttttgccg cagtcgtaac tgtcgccggt catcagcatt
    23341 gcatcaacgg attgtttttt cgccagctcg gcaatagcat cagtcagctg aatacgtccc
    23401 catgcaccag gctgagtacg ttccagttcc ggccaaatat cggcagaaag cacatagcga
    23461 cctacggcca tgatgtctga gtccagcgtc tgcggctgat ccggtttttc gataaattca
    23521 acaatgcggc tgactttacc ctcacggtcc agcggctctt tagtctggat gacggagtat
    23581 tcagagaggt cacccggcat acgttttgcc agcacctggc tgcggcccgt ttcgttgaaa
    23641 cgtgcaatca tggcagcaag gttgtaacgt agcgggtcgg cgctggcatc gtcgatcaca
    23701 acgtctggca gtaccacgac aaatgggttg tcaccaatgg caggtcgcgc acacaaaatg
    23761 gagtggccta aacctaaagg ttcgccctga cgcacgttca taatgatcac gcccggcgga
    23821 cagatggact gtacttccgc cagcagttga cgcttcacgc gctgctcaag gagtgattct
    23881 aactcataag aggtgtcgaa gtggttttcg accgcgttct tggacgcgtg agttaccagg
    23941 aggatttctt tgatccctgc agccacaatc tcgtcaacaa tgtactgaat cattggcttg
    24001 tcgacgattg gtagcatctc tttgggtatc gccttagtgg caggcaacat atgcatcccg
    24061 agacccgcta caggaataac tgcttttaaa ttcgtcatta tttcatccac ctgtaaaatg
    24121 gttgctgaat tatagcttgt tcgatttttt tcgccagcat caattaccct gaattgatta
    24181 ctgaattact tgtgatgtta cgccgcttcg ttgtggattg cagtagcatt gttcctaagt
    24241 atggctccat ttttccagga atggtcgcaa atctactccc tccgttccgg caatctaaag
    24301 ttaatctttt ccacattaac aatatggtga ttaatcctgt cgatatcgac ggagctttgc
    24361 cctctttcat tcaccgcatg aacatttgca agagacagca gtgtttcttt tttcgccata
    24421 aagacgccgc gaacgtcttt gcgcatgtcg aagttcatgc tcaatgctgg tccaactgag
    24481 gattcctgca tcacattgat atttcgcata aaaagatgtt gcggcttgtt gtgtaactcc
    24541 agcgaggcac gcttcatctc aatgttagtt agcgacacaa aggagacggc attcccggcg
    24601 gagatttgga tgccgcgcaa tttataagca agatgggtat tatccagttg aatattattc
    24661 actcggaaat tttgcggtat cgagagatat ttgcctttaa ttaccccata gccgattaac
    24721 atcccggcgc tattaatcat ttcaatatta tcaatcacga aattgtcaca accgtaaata
    24781 gcgactgtcg cgttatcaat gcccgctttc ttactgaaat ccggcgtgat attgcgggca
    24841 ttgatattac gaataacaaa atgtttacca ttttcaacat ggatcaactg ccgacaatcc
    24901 gatcccgtga tattcgccac gacaaagttt ttcactgcct ggtcttccgg gtagttgtta
    24961 tcataagtgc ttcccgcaag gcctatgccg atgccccagt tgattttgcc gttggtacag
    25021 ttgatacgct cgatgacatg gtcagatatc aaaatatcac tgtcgttaat tgccacgttc
    25081 cattcaatgg cgtcgccctg taagtcgctg aacttacagt tggtaatgtt ggcaccgata
    25141 atctggttat gaaatccctg gcgtaagatg gcgtaattag cgtggctgac agtgaggtta
    25201 tcgatggtca ggttgcgcat gacccgtttg tttttgccgc caatataaat ctgcattacc
    25261 gggccaaagc cgctcatcgc cagccctttg atggtgcagt cagaaccacg cacatccagg
    25321 gtaatgttat gcataccgcc gccctctccc cctgtcacct ggctgccgtc ctgtaagaca
    25381 aatcgccctc tgccgttgcc gcgcaggctt ccaaggatgt gtaacgtttt gccgggaggg
    25441 atgaagatgc cggtgttgat attgtcacaa acgaatccgg caggcacgac gaccgtttgc
    25501 ccttcgctga aggcttgttt aaatgaggcg atccagtcat gtgggttgta gtcgttaatg
    25561 ttaacgcttt gtcgggcggg aagtgcgcga gcgaaagggg tatggaggaa ggcaagcgcc
    25621 gagcttgccg tcaggaaggt gcgtcgggag agtgttttaa atggcatacg ttctcctcta
    25681 taaagcctgc agcaagctgg cgagttctcg attaatcacc tgctggttaa aatcgtgttc
    25741 gactttttcg cacgcacgtt tgacgaccgg agccagttcg tcggtatcca gttggctaaa
    25801 cgccgccaga cgttgtgcca gtgcgcgagc atcgttctca ggcagcagcc agccggattt
    25861 atcggcctcc accagttcag gaattccgct atgcagagta gaaaccaccg gaataccgac
    25921 cgccatcgct tccattagcg ccaccggaat gccttccata tcgccatccg cgcccgttac
    25981 cgatggcaac aggaagacat ccgcgtcgtc gagcatctcc ttcacttcat ggctcggttt
    26041 aaagcccggc atctctacca catcttccag ttgatattgt tcgatgatgg tgcgcaggcg
    26101 tcgttcccac aggccaatgc cgaggatgcg atagcgaaat gccacgccct gctctttcaa
    26161 ctgacggcag gcttcaatcg ccacatgcag gccttttttc tcggttaagc gtgcgacaga
    26221 tatgatttcc agcggcgttg cgggcgcttt caccggacgc gggctaaagc gcgtcatgtc
    26281 cacgcccata cgcgatacgg cgattttttc tctggggcag cccatttttt gcagccttcc
    26341 ggcccacaga tcgcttatcg gtaacatcag gtcgccacgg cgaaacagtt gttgatattc
    26401 gggagtgtag tggttgagca cttcccgact ggagatatca ataccgtgga agatggtggc
    26461 aattttgccg cgaatgatac ccagttcgcg tagttttgct gcggttaccc ccgccgggcc
    26521 aaaatgagcg atgaacacat cggcgcgaaa cggtgttgcg acctgaccac aaatggcaga
    26581 caaaatcagg ttccgcgact cggcaccata gcgtttgagg ttgagcgcat gccaggtatt
    26641 tttacgatga atgccacgta aggtctggct ggcgcggtgg cgcagtttcg ctactttgcc
    26701 cgcaggttcg tcctgtaacc agcgggttct ggcgacaagg ttgtatttcg tccatgccgc
    26761 gtgggtgttc tctgtatcgc ctttttgcaa agcgacaatc tccacttcaa agcccatatc
    26821 aataaacgcg gtaatttgat tgaggacgaa ggtttccgac gacagcggga atttcaataa
    26881 gaagaagccg accttcattt cacctccccg atgcgctcaa gcacagactg caccatctgc
    26941 attcccgtct gacgttcgcg actgacggct tcgttaaatc gagcgttcag caccggaagc
    27001 tggcctaagg tatccgcaac catcgcttgc aggctgccgt ccagtaaatg acgaatatca
    27061 atcgccatct ctggcagtcc cagctgctgc ataatcccgg cggatttatg ttcatagttg
    27121 atggcaatcg ccggagtagc aaagttcatt gagataatgg cggagtgcag gcgcgtaccg
    27181 acggtgagtt cacaggctcc gagaattttg cccatttcca gatcgttgag ttcatccatc
    27241 actacgtggt aacgggcagg atcgctgatg tgctggcgca gattgagcgc caccatgcgg
    27301 tcgtctttgt tatagctgtc gatacccgta caggtggaga gcgcaatcac ctgatagcct
    27361 tcatcgagaa tgcgattgac caccccggca aaggcttttt catacgcttg ttgagtggtg
    27421 ccgagacgtt tgtcgaacgg tgccagttcg cgcagggtaa ttgccactgt tttctgttgt
    27481 gcggcaacgt ccagccagtg ttgaacggca tagctggcgg tgaagtcttc ggtgtggtga
    27541 tcgaccagcc acgcggtatc gacgccatgt tccacttttg cggtggtgat atttctgcgt
    27601 ttcatcaaat caaggctgac cgattcgcgc aggatcagcg cgtcgcagtg accaaagaca
    27661 tagttcgcca gttggttaaa ttgctcatcc tggaaggggc cgacgctgtg accaatcata
    27721 aacagcggct ttttcgccat aaatgtgcaa agcgcatgtt caaactgcgg cacgccgtag
    27781 agatcgacaa aaaacgatcc gccgacctga ataatggcgt cgtaacctga cagcaggcgc
    27841 acgaagtcgg tgaatccctg ggcgatggcg atattgcgca gcttgccagt gtcggtgacg
    27901 cgtgagagca atacctgatg ctggtagcgg cgacggagga cttttttaat gcgcccgaca
    27961 acgcccgccg cgctattgtg ttgtgtcatc tgcaggaaca gcggatcgcc cattaccggg
    28021 cggttgagca gccaggaaga actgaccgga tagcggctca tcacgtccac ttcggtatgt
    28081 ggattgagga tgttgatggc atcaagtaag ccgcgcaaga tggcgctgtc gccacgattg
    28141 ccgcaagtgt ggttgcccag aataagtaat ttcatttttt cctcataaat ttgatgccag
    28201 gtgaggctgt gtttgttgtg ttcgttgccg ttttttgcct gatgcgacgc tggcgcgtct
    28261 tatcaggcct acaggtcccg agcacagaac cgtaggacgg ataaggcgtt ttacgccgca
    28321 tccggcaacc gttgtcggaa ccgaaaacag caattcaccc cgcccgtaaa agcattttca
    28381 ttttttcgct gcgacaaaac tgacgcttca cttccaccac cagcggatgg cgtgacagca
    28441 caatcatcac cacaaacgcc agcacgcccg cggctatttg caccgccagc agcatcccca
    28501 gcgccagttg ccctttcagc acaatgccca gcgcataact gaccaccagc gtcggcaacg
    28561 agagataaaa cggcagccac aaactcagga tgtactggcg ataactggaa ccaagaaccg
    28621 gtttaatcat cacgaagtaa ctcagaatgg tgttgataat ctgcaccagc aggaaaccga
    28681 gcgtgacgcc gattgcgccc gccatctgcc caccaataac aatcgccgga ataaacagaa
    28741 atgttttgaa tacgttgaat ttaaagctga tatcgacccg cgctttcgcc atcagcagcg
    28801 aaccaatcgg gttacccacg gagcgcagca gacccacaat acacagcaat tgcagcaccg
    28861 gaataatgct gttccacttt tcgccgaata ccagcggtac gaagttattc gacaccacca
    28921 tcagccccag cagcgccgga aagttgataa tccccactat cgacagcagc ttgtagaagt
    28981 taacacgcag cttttcggtg tcgtcctgaa ttttggcaaa cgccggaaac aacacgcggg
    29041 tgatgattgg gttcagcttc atcggcggta cgacggccac gttgtacgcc aggttgtatc
    29101 cccctgccac gcccgcgcca agaatacgcg ccagcaccag cgttgaaagg ttggtgttga
    29161 gatagttgat gatgctgtcc gccgtcagcc aggcaccaaa acgtaagttc ggtgccacag
    29221 acgccagcga gaagtgcaga ccggggcaat aaattttgcg tccaaagtag ccaaacagca
    29281 gtgttctcac cgcactattg accagataac cgaggatcgc ggtcatcgcc agcggccaga
    29341 aatgggcgct aactaccgta aaagtgaagc ccgccagcac cgcgctggtt tcgatcatgc
    29401 cgattttgtt gaactccagc tccttctgca tcaacgcgcg gaactgttgc ccgtggggga
    29461 tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc caggtccggg ttattcagca
    29521 cgtcgccgat gacatcactc aacaaaaaca ccgccacaca taccacaagc cccagcccga
    29581 cgttcagcca gtacagcgtg gtgagttcaa ggtgactgat ttcttttcgc tgaataatcg
    29641 agttagcgat accgaagtca gaaagcgtat ctgccagcgc gataatcacc agcgacacag
    29701 tcagcaggcc gaactggtgg ttgtcgataa tccgcgccag cacggtcatc tgcaccagcc
    29761 cgaggccgat gatgatcacc gtggcaatag ccgaccactt cgcgccgctg atggtttttt
    29821 cacgtaagct catatcaata tgccgctttg ttaacgaagc ccttgaatac cgtcaggaaa
    29881 acgattttga tatcgaacca gacgctccat tcgcggatgt actcaaggtc gaactcgacg
    29941 cgtttttcca ttttctccag cgtgtcggtt tcgccgcgcc agccattaat ctgcgcccag
    30001 ccggtaatgc ccggtttcac tttatggcgc agcatgtagc cttcaatgag ctggcgatac
    30061 tgttcgttat gcgctactgc gtgcggacgt ggaccgacaa tcgacatccc cccggtcagc
    30121 acattgataa actgcggcaa ttcgtccagc gaggtgcggc gcagaaagtt ccccacttta
    30181 gtgacgcgcg gatcgttctg cgtcgcctgg gtcaccactt tgtcgttctc catcactttc
    30241 atggaacgga acttccacac tttgatcggc ttgccatcca taccatagcg agtctggcgg
    30301 aaaataaccg gccctggtga actgagtttc accgccagcg caatacagca cagcaccggg
    30361 gagatcagca gcagaataag cgtcgccagc acaatgtctt ccgcacgttt tagcaggcgg
    30421 ttaacccccg aaagcggcgt gtcatacagc ggcaccaccg gaacgccgtt catctcttcg
    30481 agacgtgaat ggagaatgtt gaaggtaaag acgtcgggga tcagcagcac cgaacaggtg
    30541 gtatccgcca gttgatggac cagttttttc actcgtgcgc cgtcgcacat ttgcatcgcg
    30601 atatagacgt tatgaatctt gcccgctttt gcatcctcga ccagctgttg caggttaccc
    30661 gcccagtcgt tagaaacgcc gcccggtttc gggtcgtggt aaacgcccac cacttcaaac
    30721 cctaaccacg gctgattacg gaaactctcc atcagcattt gtccggcggc taaatccccc
    30781 gctacggcga ccatccgctt gttgtagcca tgattacgca gccagcccgc cccaatgcga
    30841 atacatgaac ggcaaaccac cagtccgatg ctggtcagcc cataccacgc cagccagatt
    30901 ttcagttgcg tgtcgaaatc attgttgaac gccaccagtc cggcgctgaa aatcacgctt
    30961 aaggtccagt tttgtagcaa cagggcaaat tctgtcgctg cccgaacacc gcgccatgag
    31021 cgataaaaat cggtgatgcc gcccagcatc tggaacacca ccagcgtaat cagcgccacc
    31081 aacaggtgca tgtagaggaa tgacagtccg ctgacttcgc aaaccagcca tagcccggca
    31141 aacatgatgg tgatatctga aaagcgttgc accatagaga ttaacgatgc attggttttc
    31201 gctcgctcgc gcttttttag atttgtcatc gttgttcctg ttattagccc cttacccgca
    31261 gtggggtaag ggaagatccg acattactcg ttcagcaacg tcagcagagt tcgcgttcgc
    31321 gcttccatca gcggcacatc accgcgcgat tccacattca ggcgcaccac cggttcggta
    31381 ttggaggtgc gcaggttaaa gcgccagtcg gcaaaggtca tgctgatgcc atcgctgcga
    31441 tccaccgcca gcgcctcacg gctaaaatgc tgttccacgc ggttaatcgc ctcaacgggt
    31501 tgcgccagtt tgctgttgat ctcaccgctt gccggaaacg ccgccatccg gtcgcgcacc
    31561 agttcgccca gcgtttttcc tttcaggcac accagttcgg cgaccagcag ccacgggatc
    31621 atgccgctgt cgcagtaagc gaaatcacgg aagtaatggt gggcgctcat ttcgccaccg
    31681 tagatggcgt cttccttgcg catccgttct ttaataaagg cgtgtccggt tttcgacatt
    31741 accggcgtgc cgcctgcagc agtcaccaca tcaatagtgt tccaagagag acgcggatca
    31801 tggatgatct tcgcgccggg atttttttcg aggaacgctt ctgccagcag gccgacaatg
    31861 tagtagccct cgataaactg ccctttttcg tcaaacagaa aacagcggtc aaaatcgcca
    31921 tcaaaggcaa tgcccatatc cgcgccgtgt ttgatgaccg cattgcgggt gtcgtcgcga
    31981 cattccggca gcagcgggtt aggaataccg ttggggaaat tgccgtccgg tgtgttgtgt
    32041 actttgatta attccacggg cgcgccgagg gctttaaagc gggcttcaat ggcgtccacc
    32101 accggacccg ctgcgccatt cccggagttg atcaccagct tgagcggcgt gagatttttg
    32161 acattgatat aaccgaacag gtgatcaacg taagcgtcac gcaggttgat ttgctgatag
    32221 cgaccgcgtt tggtttcatc gacgggagga aagtcgttgg cttcagccag acgctggacg
    32281 tcgcgcagtc cggtatcacc gctgatcgtg cgagccccct cgcgcaccag cttcatgccg
    32341 ttgtaatcca tcggattatg gctggcagta acttcaatgc cgccatccac gccgagatgg
    32401 aacgtggcga aatagatctc ttcggtgccg gacataccga tatccagcac atcgacgccc
    32461 gcatcctgta aaccttttgc cagcgccagt tttaaggttt cgctggtgag acggacatca
    32521 ccgcctaaca caatggtttt cggtttgaga aattcgccat aggcgcgacc aatgcgccag
    32581 gcgatatctt cattcagttc ttcgcctaat ttcccgcgaa tatcataggc tttaaagcag
    32641 gttaattttt tcatgtcgtt accctttttc aggcaatagt tggccctgac cgaaacggcc
    32701 agttttattt ttgtcgttat ttacaccgcg gtttcgcatt cattgcctga tgcgacgctg
    32761 ccgcgtctta tcaggcctac aaaaccgaac cgtaggtcgg ataaggcgtt cacgccgcat
    32821 ccgacaaaca gcgcctgatg cgacgtttac acccgtccgt agcgatccgc gaagcgcacc
    32881 acatcatcct cttcgagata agagccggag cgcacttcaa ttaaatcgag cggaattttc
    32941 cccgggtttt ccaggcagtg cgtcgccccc agcggaatat aaatggactc gttttcacca
    33001 agcagtttga tatcaccatc aatggtgact tttgccgttc ccgcgaccac tacccagtgt
    33061 tccgcgcggt gatggtgcat ctgtaccgac aaaccctcgc ccggtttcac ggtgatgcgt
    33121 ttcacctggt agcggtcgcc cgcgtcgata gagtcatatt tgccccacgg acgatacact
    33181 tcgcgatgca cccgatgctc atggcgacca tcggctttga tctgctcgac cacttttttc
    33241 acatcctgta ccgcgttacg gtcggcaatc agcaccgcat ctttggtctg caccactacc
    33301 agatctttca ccccgacggt ggtgaccagg cctgattcgg catacacata gctgttttca
    33361 gttttgtgat taatcacatc gccgtggcaa acgttgccct cggcggtgtg ggcgctgatc
    33421 tcccataatg aagaccagga accaacatcg ctccagcccg catccatcgg caccacaaca
    33481 gcatctgccg taggttccat gaccgcgtaa tccaccgact cttccgggca ggcgagaaac
    33541 gcttcttcat ccacacgaat aaaatcgaga tccggatcga cggcgctcat cgctttttcg
    33601 caggcatcga ggatatccgg gcgatatttt ttcagttctt cgagatagcg tccggcgcgg
    33661 aacaggaaca tgccgctgtt ccagtaatat tcgccgcttg ctacataggc ctgggcggtt
    33721 tccagattcg gtttttcgac aaactgcgcc acttcaaagg ccaccgtatc ctgctcaccc
    33781 acagacactt caccgcgacg aatatagcca taaccggttt ctggtagatc cggcacaatg
    33841 ccaaaggtca ccagcttgcc cgcttcggca taaggcatgg cattacgcac ggcggcacgg
    33901 aacgcgtctt catcggcaat cacatgatcc gccgccaata ccagcattaa cgggtcgctc
    33961 tccgggctat gacgttttgc cgccagcgcc gccagtgcaa tggcaggtgc agtgttacgc
    34021 cctgccggtt cgagaataat gttctcggtg agtttgttca gttgacgcag ctgttccgcg
    34081 acaataaagc ggtgctgctc attgcaaatc accaccgggc tttcgcactc cacgccgttc
    34141 aggcggcaga tggtggtttg cagcatggtg agatcgcctt tcaggcataa aaactgcttg
    34201 gggtaaagta cgcgggaaag cggccataaa cggctaccgg agccacctgc catcacaact
    34261 ggatagagtt tcgactgcgc cataattatc cccgaatatc atttataaat tgacgtaaca
    34321 cgttctcttt atcgagcgtg cgttcggcat attcacgtgc caccgtgtta tgtttgggca
    34381 gcaggagcgc ctgacgaatc cccgccacca gcgcctcgac cgattccggt tctacgcaaa
    34441 ccgcaatacc cggaaaggtt tcgcaaagct gccccagttc tgtgtgggcc tctgcggtaa
    34501 tcaccgcgtt accgcctacc gccagaatat tggtcagttt cgacggcaat acggcatctg
    34561 ccgcgccgcg tttttgcacc accagatggc aatcgcccat cttcagcagt gcaggtaaag
    34621 cgtcatacga ttgcagcggg aaaaattgca tgttgcgcag tccacgctgc tgcgccattt
    34681 tttccagccg cgctttgccg ccgccctgcc cgacaatggc aaaaatcagc ggttcatcgc
    34741 gcaggagatc ggcagcttca ataacgtttt ccagcccctg cttttcacca atattgccgg
    34801 agtaaagaat gatttttttg ttatccggca ggccaagctg gttacgaagg gcatcaacat
    34861 cggcgtctgc aacatgctga aaacgggcaa tttccgacca gttggggaag aagatgacgt
    34921 tatccgccgc cacgcctttt tcgatggctt tattcatcat cgaacgcgaa atcgtggaaa
    34981 cgttatcgac gttatgcagt ccgctacgtt cgaacgccgt tgccagctgt gccactttgc
    35041 cgcctttgcc ttttccggca aggcccagcc ccagcatggc gtccacttcg taatcctgaa
    35101 tatgcagcac ggtacgcgca ccagagagct tcgccagcag gcgcattccc ggcgtgcaaa
    35161 acagcgttgg cactacgcca ataatgcgat ccggcttcca gcgacgttgc gccatcagtg
    35221 gaaagaaact gctgacggca aaactgccga gatgcaacaa gcgtttcagg gtgctcggct
    35281 gttttggcac gtacagcggg cagcgccaca ccgtggcggc cccttcttct cgtttgtagc
    35341 gccaggcgga atagttctca cccacctgcc attgcgggta gtaaggcggt gcggtaatga
    35401 cccgcacctc atgaccttgt gccgccaacc attccaccat ctcgccggtg tatttgccga
    35461 tgccggttaa ctccggcgag tagttaatgc cgtagaccag tattttcata atccgggtac
    35521 tccggcacgc ttctcagcga gaaaataggc tcggctgtta gcgtgaacat cgttactggc
    35581 gagcagcgcg tccggcgtca gccagcggta atcgtcatgc tgctcatccg gcagtaacag
    35641 ctcttcttcc gctactctga agcgaaaacc gagcaccaca tagtgagtgg tgaaatccgt
    35701 gccggagaag ttatcgtcat aaaaatgctg ccagacaccg taaaactggc ctgctgttat
    35761 cggcagacgc agccccagtt ccgccatcgt cagccgctca aatgcggctt ccagcgtttc
    35821 gtctttctgc acgcgccctc ccggcacaaa ccagtaaccc tgcgccgggc ggttggttct
    35881 tttgccaagc agaaactcgc cgcgactgtt ctcgacaata aagtcgagag agacaagcgg
    35941 tgtggagcgc actacagtgg caaagtcttc ctgacgtaaa aacatcattc cccccgaaag
    36001 cggtcttgat tctcaaggaa ccactggtaa gtgctggcaa gccccgcttc cagtgagatt
    36061 tcgtgatacc agccaagctg atgcaggcgc gtcacatcca gcagtttgcg cggcgtacca
    36121 tccggtttgc tggcatcaaa aaccacccga cctttgtaac ccaccacttt ggcgatggtt
    36181 tgcgccagct cgcggatagt gcagtcaacg cccgtgccga cgttaatgtg cgacagcatc
    36241 ggttgggtat tctccagcca gacttcatgc gccagctcca tgacatgaat gctcgccgcc
    36301 gccatatcat cgacgtgcag gaattcacgc atcggtgtac cgctgcccca taccaccacg
    36361 tccggcgcat tctgtgccgt cgcctcgtgg aagcgacgca gcaatgctgg tatcacatgg
    36421 gagttactcg ggtggaagtt gtcatgcggc ccgtacaggt tggtcggcat gacagtgcgg
    36481 taatcgcgtc catactggcg gttgtaagat tcgcacagtt tgatcccggc gattttggca
    36541 atggcgtaag gctcgttggt cggctccagc gtgccctgca ataactcgct ttctgccatc
    36601 ggctgttttg ccagtttcgg gtagatgcag gacgatccga gaaacatcag tttgttcacg
    36661 tcgttctgat gcgcggcgtg aatgatgttg ctctcaatca tcatgttctg gtagatgaaa
    36721 tccgccggat aggtgttgtt ggcaacaatg ccgcccactt tcgccgccgc cagatagacc
    36781 tggtcaatgc gttcgctggc aaagaaatca tgcacggcgc ggctgtccag caggttcagt
    36841 tcatcgcggg tacgtaatac cagctccaca tcaccgcgct gttcgagctg ccgcttgatg
    36901 gcagaaccga ccatcccaca atggccagcg ataaaaatgc gttgtttact catgcttatg
    36961 actccagcgc gatcgccacg tcgtagccgt gagatttcag cagagagtgt tttttcgccg
    37021 cttcgaggtc attagccacc atttcagaca ccatctctct gagggtgatt tccggtttcc
    37081 agcccagttt ttcgtgcgct ttggtcgggt cgccgagcag cgtttcaact tctgccggac
    37141 ggaagtaacg cggatcaacg gcgataatca catcacccgg tttaacgccc ggcgcgtcat
    37201 gcccggtgac ggaaaccaca atgcccttct cttcaacacc cgtgccttca aagcgcagtt
    37261 tgatgcccag ctgtgctgcc gccatttcca caaactgacg tacggagtac tgaacgccgg
    37321 tcgcgataac gaaatcttcc ggctgttcct gttgcagcat catccactgc atttttacgt
    37381 agtctttggc gtggccccag tcacgcaggg aatccatatt gccgaggtac aggcacgact
    37441 ccagcccctg ggcgatgttg gcgattgcgc gggtgatttt gcgggtaacg aaggtttcgc
    37501 cgcggcgcgg ggattcatgg ttgaagagaa ttccgttaca agcgtacatg ccgtaggatt
    37561 cgcggtagtt aacggtgatc cagtaggcgt acagtttggc gaccgcatac ggagatcgcg
    37621 ggtagaacgg cgtggtttct ttctgcggaa tttcctgcac cagaccatac agttcagagg
    37681 tggaagcctg atagaaacga gttttctttt ccagaccgag gaagcggatc gcctccagca
    37741 ggcgcagcgt acccatcgcg tcgacgtcag cggtatattc tggtgactca aaagagaccg
    37801 caacgtggct cattgcgcct aggttgtaca cttcatccgg ctgtacttca cgcaaaatgc
    37861 gcgtcaggtt agaggtatca ctcaggtcgc cataatgcag atggaatttc gggttgcagg
    37921 tgtgcggatc ctgataaatg tgatccacgc gctcggtgtt gaatgacgat gcgcgacgct
    37981 taataccatg cacctcgtaa cctttttcca gcagaaactc tgccaggtaa gaaccgtctt
    38041 gtccggttac accggtgatg agagcgactt ttgacatgtc ttattcctct gtatttttga
    38101 atttattcag tttcaacgcg ttcgcgtatc accactgcgg gattcccccg gcaaaccaca
    38161 tttgccggaa gcgatttaaa aacactgctt cgtgcaccca cgacggtgcc gtcgccgatt
    38221 gtgacgcctg gggcaacaaa gacatcggtt gccagccagc atttctcgcc aatcacaata
    38281 ggcgtggcgt taatggtgaa atgttgactt gcatggtcgt ggctaccggt gcataaataa
    38341 cttttttgcg atatcaccga atgtgcgcca atggttattt caccgagggt atataaattg
    38401 acgtcatcgc caacccacgc gtaatcacct aaggttaatt tccacggata ggtaattttt
    38461 actgacggac gaataactac gttttttcct atttttgctc cgaataaacg taataaaaaa
    38521 gcccgccagc gatacaatac ttgtggcgac caggcaaata ttgttgcctg tactgcccac
    38581 cataattgca ctttaatagc gttgccgccc cggaaccctt tcggcaccga gaaaccgctt
    38641 aaatcttgca ttggttttcc ttatattcag actttgttat ataaggcttt agtcttacct
    38701 gtcgtacgtt ggcgtaaatg ccaggataat tctgcccaga agccaggtac atgtaatatc
    38761 tgccgttgga cttttttggc gtcagcaca
//
LOCUS       DAJTUM010000027        38524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTUM010000027 DAJTUM010000000
VERSION     DAJTUM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..155
                     /locus_tag="OO471_001536"
     CDS             <1..155
                     /locus_tag="OO471_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6242852.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO471_001537"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO471_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6242853.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO471_001538"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO471_001538"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6242854.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO471_001539"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO471_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO471_001540"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO471_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6242855.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO471_001541"
     CDS             complement(5626..7134)
                     /locus_tag="OO471_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6242856.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO471_001542"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO471_001542"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6242857.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO471_001543"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO471_001543"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6242858.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO471_001544"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO471_001544"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6242859.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO471_001545"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO471_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6242860.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO471_001546"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO471_001546"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6242861.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO471_001547"
     CDS             complement(14283..15602)
                     /locus_tag="OO471_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6242862.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO471_001548"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO471_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6242863.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO471_001549"
     CDS             16699..17034
                     /locus_tag="OO471_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6242864.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO471_001550"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO471_001550"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6242865.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO471_001551"
     CDS             complement(17790..19172)
                     /locus_tag="OO471_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6242866.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO471_001552"
     CDS             complement(19358..20302)
                     /locus_tag="OO471_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6242867.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO471_001553"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO471_001553"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6242868.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO471_001554"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO471_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6242869.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO471_001555"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO471_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6242870.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO471_001556"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO471_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6242871.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO471_001557"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO471_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6242872.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO471_001558"
     CDS             complement(25947..26768)
                     /locus_tag="OO471_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6242873.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO471_001559"
     CDS             complement(26868..26951)
                     /locus_tag="OO471_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242874.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO471_001560"
     CDS             complement(27044..27379)
                     /locus_tag="OO471_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6242875.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO471_001561"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO471_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30065
                     /locus_tag="OO471_001562"
     CDS             29136..30065
                     /locus_tag="OO471_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6242876.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAKEPFVFFDPHVGTGLY
                     DDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAE
                     EDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30200..31420
                     /gene="mlc"
                     /locus_tag="OO471_001563"
                     /gene_synonym="dgsA"
     CDS             30200..31420
                     /gene="mlc"
                     /locus_tag="OO471_001563"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6242877.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31545..32240
                     /gene="bioD"
                     /locus_tag="OO471_001564"
     CDS             31545..32240
                     /gene="bioD"
                     /locus_tag="OO471_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6242878.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO471_001565"
     CDS             complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO471_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6242879.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO471_001566"
     CDS             complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO471_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6242880.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34301..35155)
                     /locus_tag="OO471_001567"
     CDS             complement(34301..35155)
                     /locus_tag="OO471_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6242881.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO471_001568"
     CDS             complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO471_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6242882.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35785..37823)
                     /locus_tag="OO471_001569"
                     /pseudo
     CDS             complement(35785..37823)
                     /locus_tag="OO471_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37884..>38524)
                     /locus_tag="OO471_001570"
     CDS             complement(37884..>38524)
                     /locus_tag="OO471_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR6242883.1"
                     /translation="PALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
                     KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRT
                     HSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPG
                     VSAMGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9797 a         9340 c         9876 g         9511 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaagcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgcgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagagt acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgtgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt
    29761 ttgatccgca cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc
    29821 atttgacgcc cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt
    29881 ccgccggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa
    29941 tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga
    30001 aacatcatga acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca
    30061 ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta
    30121 aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg
    30181 aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa
    30241 gcagaccaac gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat
    30301 cgatctttcc cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat
    30361 gctcgaagca cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc
    30421 ggcggtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg
    30481 cggggagatt ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca
    30541 ggaactggcg ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca
    30601 gttttttatc cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc
    30661 gggaattatt gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa
    30721 agagatgccg ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca
    30781 tgatatcagc gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga
    30841 tgtgattcag gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct
    30901 gctacacgca ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg
    30961 taaacgctgt tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat
    31021 tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc
    31081 gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat
    31141 cattatcggg gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa
    31201 cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt
    31261 catctcagac agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga
    31321 gagtactcag ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat
    31381 gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc
    31441 aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt
    31501 attttcctag tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct
    31561 ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag
    31621 cgttagcctc ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag
    31681 agacacccga agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg
    31741 aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc
    31801 acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag
    31861 tcgatcatgt cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc
    31921 cactctctga atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg
    31981 aaggttgcat taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc
    32041 tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg
    32101 atgtgctggg taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg
    32161 ccgaacagcg tgaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg
    32221 tagatagagt cacggtgtaa cgtctgcgaa attaccgacg caattacgca ggcaatcaat
    32281 aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc
    32341 ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta
    32401 atttcttcgc catcagggaa ccataatccc aggctacgac catacaacat gccaatggca
    32461 agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc
    32521 agcacggcac acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg
    32581 gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa
    32641 agcagaccca cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc
    32701 acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc
    32761 agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt
    32821 acgttgtaga gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg
    32881 gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct
    32941 ataaataaac tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca
    33001 caggcgatcc ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg
    33061 gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact
    33121 accagcagcg aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt
    33181 tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag
    33241 cccatcagca acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag
    33301 gaggaaaggt ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac
    33361 tccagtagca gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg
    33421 actgttgcga taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga
    33481 tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac
    33541 cgcaaaagca gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc
    33601 gaatcgcgcg taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt
    33661 taaccgcgac aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt
    33721 cacccagtgc acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg
    33781 ttgaccacgg aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct
    33841 ccgccaacca cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct
    33901 tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc
    33961 caaacagcac agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc
    34021 acgggccgac aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa
    34081 aagccgtcac cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt
    34141 cactggtgag taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca
    34201 gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc
    34261 ctaacatcct gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc
    34321 ctactgtcat gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga
    34381 ggattagccc cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa
    34441 gccagcatcc aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat
    34501 ctggtaccag tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt
    34561 gcagcacaat cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg
    34621 tggtattaaa tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca
    34681 acaataccgt caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt
    34741 cgatttgata cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa
    34801 gtaaccagag tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata
    34861 gtccgccaac ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc
    34921 cgattcgatt cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa
    34981 taaacccgat gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt
    35041 ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc
    35101 attgacccaa tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac
    35161 acttcctccg gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta
    35221 ggtttgataa cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc
    35281 agagtgccgt gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg
    35341 caggattcga cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac
    35401 ttcgtcatgt gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg
    35461 cagtagcggc agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta
    35521 tgcattgcgc cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag
    35581 atggagagat aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg
    35641 ccgccagcat attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct
    35701 ttgcacgcca gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca
    35761 tattgtgtgg tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga
    35821 cgggtttccc tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc
    35881 gtgatcgacc cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt
    35941 aacgccaggc agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt
    36001 aaatacccgc acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat
    36061 ccacacctct tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt
    36121 acgcgctttg gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt
    36181 gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag
    36241 ataaggtctt cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc
    36301 gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc
    36361 acctcatggg tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac
    36421 cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg
    36481 gtcattttcg taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta
    36541 acaggattct cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca
    36601 cctgagttgc cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg
    36661 cgggacgttt gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca
    36721 ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg
    36781 atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag
    36841 gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca
    36901 caatatttgt cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc
    36961 gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg
    37021 gcagctgtgt cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg
    37081 gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt
    37141 ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca
    37201 ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg
    37261 agaaaacccc cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg
    37321 ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc
    37381 cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta
    37441 ccttcaccgc gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc
    37501 cgtcggcgga tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg
    37561 tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc
    37621 agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga
    37681 gcctctgccg cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg
    37741 agactgccaa gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc
    37801 gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg
    37861 ccagaattta tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc
    37921 gggtttccct tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca
    37981 tggtcgattt tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta
    38041 actccaggga gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta
    38101 aacacccgca ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc
    38161 cacacctcct gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta
    38221 cgggatttgt aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc
    38281 cagccttcaa aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt
    38341 tcccaggtac gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc
    38401 gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca
    38461 ttgggatctt tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc
    38521 ggat
//
LOCUS       DAJTUM010000028        37628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTUM010000028 DAJTUM010000000
VERSION     DAJTUM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..176)
                     /locus_tag="OO471_001571"
     CDS             complement(<1..176)
                     /locus_tag="OO471_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6242884.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO471_001572"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO471_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6242885.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO471_001573"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO471_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6242886.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO471_001574"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO471_001574"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6242887.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO471_001575"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO471_001575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6242888.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO471_001576"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO471_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6242889.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO471_001577"
     CDS             complement(5180..5422)
                     /locus_tag="OO471_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6242890.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO471_001578"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO471_001578"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6242891.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO471_001579"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO471_001579"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6242892.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO471_001580"
     CDS             complement(8339..9313)
                     /locus_tag="OO471_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6242893.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO471_001581"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO471_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6242894.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO471_001582"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO471_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6242895.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO471_001583"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO471_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6242896.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO471_001584"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO471_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6242897.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO471_001585"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO471_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6242898.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO471_001586"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO471_001586"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6242899.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO471_001587"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO471_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6242900.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO471_001588"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO471_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6242901.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO471_001589"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO471_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6242902.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO471_001590"
     CDS             23180..23383
                     /locus_tag="OO471_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6242903.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO471_001591"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO471_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6242904.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO471_001592"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO471_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6242905.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO471_001593"
     CDS             26388..26660
                     /locus_tag="OO471_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6242906.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO471_001594"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO471_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6242907.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO471_001595"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO471_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6242908.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO471_001596"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO471_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6242909.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO471_001597"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO471_001597"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6242910.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO471_001598"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO471_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO471_001599"
     CDS             complement(31907..32305)
                     /locus_tag="OO471_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6242911.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO471_001600"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO471_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6242912.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OO471_001601"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OO471_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6242913.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OO471_001602"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OO471_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6242914.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OO471_001603"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OO471_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6242915.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OO471_001604"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OO471_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6242916.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="OO471_001605"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="OO471_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6242917.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9414 c         9901 g         9198 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatggc ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAJTUM010000029        36387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTUM010000029 DAJTUM010000000
VERSION     DAJTUM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1181)
                     /locus_tag="OO471_001606"
     CDS             complement(<1..1181)
                     /locus_tag="OO471_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6242918.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     A"
     gene            complement(1328..1404)
                     /locus_tag="OO471_001607"
     tRNA            complement(1328..1404)
                     /locus_tag="OO471_001607"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1368..1370),aa:Pro,seq:tgg)
     gene            complement(1447..1533)
                     /locus_tag="OO471_001608"
     tRNA            complement(1447..1533)
                     /locus_tag="OO471_001608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Leu,seq:cag)
     gene            complement(1554..1629)
                     /locus_tag="OO471_001609"
     tRNA            complement(1554..1629)
                     /locus_tag="OO471_001609"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1594..1596),aa:His,seq:gtg)
     gene            complement(1688..1764)
                     /locus_tag="OO471_001610"
     tRNA            complement(1688..1764)
                     /locus_tag="OO471_001610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1728..1730),aa:Arg,seq:ccg)
     gene            complement(1867..3252)
                     /locus_tag="OO471_001611"
     CDS             complement(1867..3252)
                     /locus_tag="OO471_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6242919.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3443..4183)
                     /gene="rffM"
                     /locus_tag="OO471_001612"
     CDS             complement(3443..4183)
                     /gene="rffM"
                     /locus_tag="OO471_001612"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6242920.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4186..5538)
                     /gene="wzyE"
                     /locus_tag="OO471_001613"
     CDS             complement(4186..5538)
                     /gene="wzyE"
                     /locus_tag="OO471_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6242921.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5535..6614)
                     /gene="rffT"
                     /locus_tag="OO471_001614"
                     /gene_synonym="wecF"
     CDS             complement(5535..6614)
                     /gene="rffT"
                     /locus_tag="OO471_001614"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6242922.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6611..7861)
                     /gene="wzxE"
                     /locus_tag="OO471_001615"
     CDS             complement(6611..7861)
                     /gene="wzxE"
                     /locus_tag="OO471_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6242923.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7863..8993)
                     /gene="rffA"
                     /locus_tag="OO471_001616"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7863..8993)
                     /gene="rffA"
                     /locus_tag="OO471_001616"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6242924.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8998..9672)
                     /gene="rffC"
                     /locus_tag="OO471_001617"
                     /gene_synonym="wecD"
     CDS             complement(8998..9672)
                     /gene="rffC"
                     /locus_tag="OO471_001617"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6242925.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9650..10531)
                     /gene="rfbA"
                     /locus_tag="OO471_001618"
     CDS             complement(9650..10531)
                     /gene="rfbA"
                     /locus_tag="OO471_001618"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6242926.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10550..11617)
                     /gene="rffG"
                     /locus_tag="OO471_001619"
     CDS             complement(10550..11617)
                     /gene="rffG"
                     /locus_tag="OO471_001619"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6242927.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11617..12879)
                     /gene="wecC"
                     /locus_tag="OO471_001620"
     CDS             complement(11617..12879)
                     /gene="wecC"
                     /locus_tag="OO471_001620"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6242928.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12876..14006)
                     /gene="wecB"
                     /locus_tag="OO471_001621"
     CDS             complement(12876..14006)
                     /gene="wecB"
                     /locus_tag="OO471_001621"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6242929.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14062..15108)
                     /gene="wzzE"
                     /locus_tag="OO471_001622"
     CDS             complement(14062..15108)
                     /gene="wzzE"
                     /locus_tag="OO471_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6242930.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15120..16223)
                     /gene="wecA"
                     /locus_tag="OO471_001623"
     CDS             complement(15120..16223)
                     /gene="wecA"
                     /locus_tag="OO471_001623"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6242931.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16463..17722)
                     /gene="rho"
                     /locus_tag="OO471_001624"
     CDS             complement(16463..17722)
                     /gene="rho"
                     /locus_tag="OO471_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6242932.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17807..17908)
                     /gene="rhoL"
                     /locus_tag="OO471_001625"
     CDS             complement(17807..17908)
                     /gene="rhoL"
                     /locus_tag="OO471_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6242933.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18049..18378)
                     /gene="trxA"
                     /locus_tag="OO471_001626"
     CDS             complement(18049..18378)
                     /gene="trxA"
                     /locus_tag="OO471_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6242934.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18509..19774
                     /gene="rhlB"
                     /locus_tag="OO471_001627"
     CDS             18509..19774
                     /gene="rhlB"
                     /locus_tag="OO471_001627"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6242935.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19908..21392
                     /gene="gppA"
                     /locus_tag="OO471_001628"
     CDS             19908..21392
                     /gene="gppA"
                     /locus_tag="OO471_001628"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6242936.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21439..23460)
                     /gene="rep"
                     /locus_tag="OO471_001629"
     CDS             complement(21439..23460)
                     /gene="rep"
                     /locus_tag="OO471_001629"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6242937.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23547..23828
                     /gene="ppiC"
                     /locus_tag="OO471_001630"
     CDS             23547..23828
                     /gene="ppiC"
                     /locus_tag="OO471_001630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6242938.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23874..25349)
                     /gene="ilvC"
                     /locus_tag="OO471_001631"
     CDS             complement(23874..25349)
                     /gene="ilvC"
                     /locus_tag="OO471_001631"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6242939.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25499..26389
                     /gene="ilvY"
                     /locus_tag="OO471_001632"
     CDS             25499..26389
                     /gene="ilvY"
                     /locus_tag="OO471_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6242940.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26386..27930)
                     /gene="ilvA"
                     /locus_tag="OO471_001633"
     CDS             complement(26386..27930)
                     /gene="ilvA"
                     /locus_tag="OO471_001633"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6242941.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27933..29783)
                     /gene="ilvD"
                     /locus_tag="OO471_001634"
     CDS             complement(27933..29783)
                     /gene="ilvD"
                     /locus_tag="OO471_001634"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6242942.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29848..30777)
                     /gene="ilvE"
                     /locus_tag="OO471_001635"
     CDS             complement(29848..30777)
                     /gene="ilvE"
                     /locus_tag="OO471_001635"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6242943.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30797..31060)
                     /gene="ilvM"
                     /locus_tag="OO471_001636"
     CDS             complement(30797..31060)
                     /gene="ilvM"
                     /locus_tag="OO471_001636"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6242944.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31057..32703)
                     /locus_tag="OO471_001637"
     CDS             complement(31057..32703)
                     /locus_tag="OO471_001637"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6242945.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32706..32756)
                     /locus_tag="OO471_001638"
     CDS             complement(32706..32756)
                     /locus_tag="OO471_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242946.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32842..32940)
                     /gene="ilvL"
                     /locus_tag="OO471_001639"
     CDS             complement(32842..32940)
                     /gene="ilvL"
                     /locus_tag="OO471_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6242947.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33292..34771
                     /locus_tag="OO471_001640"
                     /pseudo
     CDS             33292..34771
                     /locus_tag="OO471_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34796..35134)
                     /gene="maoP"
                     /locus_tag="OO471_001641"
     CDS             complement(34796..35134)
                     /gene="maoP"
                     /locus_tag="OO471_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6242948.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35253..36092
                     /gene="hdfR"
                     /locus_tag="OO471_001642"
     CDS             35253..36092
                     /gene="hdfR"
                     /locus_tag="OO471_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6242949.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36188..36263)
                     /locus_tag="OO471_001643"
     tRNA            complement(36188..36263)
                     /locus_tag="OO471_001643"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36228..36230),aa:Trp,seq:cca)
     gene            complement(36272..36348)
                     /locus_tag="OO471_001644"
     tRNA            complement(36272..36348)
                     /locus_tag="OO471_001644"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36312..36314),aa:Asp,seq:gtc)
BASE COUNT         8890 a         9953 c         9171 g         8373 t
ORIGIN      
        1 tgcgccagca aatcagccat tgctttaaga tatggcggta gatggcggaa ataacgctga
       61 tacccggcac acaaataatt cagccccggt ttgccgctgg catcgagcat gaagcggtgt
      121 ttcgggcagc ctccccaaca tgcttttaac acgttacaac tgcgacactg cgccggtaat
      181 tgcttaaatt tatcttcacc aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt
      241 gtttgctgat gcatattccc cagccgatat tgcggataaa catagtgatc gcaggcgtaa
      301 acgtcgccgt tgtgctcaac aatcaccgag cgcccacagg ttggctgatg atggcaaacc
      361 gcacccggcg caccgacaaa attggcaaac gcccattcga tattcatcac gaaaatcttg
      421 ccgacgtcgc gtttgatcca gtggtcaaaa atcgccacca gaaattcacc aaattcatcg
      481 gggcgcacag accattccgt cagttccccc tgaatatcac caggcgcatg tagtttcagt
      541 ccatcgcgga cagctgtttc atcagccagg cgctcgacca ccggaataaa ctggatgaat
      601 tcgactcccg catcgcacaa aaaatcataa acctgtaacg gttgctgcgc gctggtgcgg
      661 ttgacgcaga ccagcacgtt atagtcgaca tgatgttttt gcaggagcgt cagggcacgc
      721 atcaccagtt tatgcgtggg tctgccgcct ttggtcacgc gatattgatt gtggatctca
      781 gccggaccat ccagcgataa cccaacaaga aaattatttt ctgccagaaa tgcacaccat
      841 tcgtcatcga gcagcacgcc gttagtctgg aaactgttac ttatcttcct gccagcacca
      901 tatttcgcct gtagcgccac ggcacggcgg taaaactcca gcccgagtag cgttggttcg
      961 ccgccctgcc aggtaaaagc cacttcgttt tgcagttcgc tggcggcgat atagtgacgg
     1021 atatacgctt ccagcgtgtc atcgtccata tgcgtgactg gcttttcgcg gtaaagggat
     1081 tgtttttcga gataaaaaca gtagtcacag ttcagattac aatcggaacc gctcggtttc
     1141 gctatcacat gaaaagcacg cgttggaacc tgttgcagca tggtcgctcc ttgcctgaag
     1201 tcatccttca atacgttaag ggcgaaatca taccgtgggg caatgagtta ttacgggcgt
     1261 ttattgcgaa gggagggtga cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt
     1321 tcaaaattgg tcggcgagag aggattcgaa cctccgaccc actggtccca aaccagttgc
     1381 gctaccaagc tgcgctactc gccgaaatac tgctttttga atttttagtt caattcttta
     1441 aagtcgtggt gcgagggggg ggacttgaac ccccacgtcc gtaaggacac taacacctga
     1501 agctagcgcg tctaccaatt ccgccacctt cgcattgtca caacttctaa taatggggtg
     1561 gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta ccaactgagc
     1621 tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct caagcgctgg
     1681 gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg agggcagcgc
     1741 tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat acggacttcc
     1801 cacccctctg tctagtccct ttttaaataa attgctcgtt tggttacggt ttgtgcgttt
     1861 tgctgcttat tcctccagtt tatgcgcttt accgtggcga ttaagcccaa aaactttata
     1921 aatcgccgtc accgccagca taaagatgat gccgacaaac agcgacatac gcgtatcttc
     1981 attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt agttggccca
     2041 cgggaacagg attgagcgga acggatggct ggcaatcgcc gctttatgtg cacgccgaaa
     2101 acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca cgctggcact
     2161 gtagacgtag acaaacacac gctgcggatt gggaatgatg tagttcaggc atgagccaat
     2221 tagcagaata gcaatagata ctgccacacc cgcaaccggt acgccgtgac gggaaacttt
     2281 tgccattgcc gccggtaact gacggttttt cgccagtgcg tagagcatac gtccgcaact
     2341 gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt tgataatgcc
     2401 cgccgctgcg gtaataccga ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc
     2461 aatttcattc cacgggaaga tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag
     2521 gatccgccac agcactttgc ctacggcact gcgcagcgtc acctgcggat tcttcgcttc
     2581 accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa tacacagagc
     2641 ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa
     2701 accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccaagc caatgacaat
     2761 catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt cgccgtacaa
     2821 ccgtaccgcc gccagattcg ccaacgccac cagcgccacc gcgatcaatg cgggtatcca
     2881 ctgcgccatc tccggggacc aaaactggac ataaacgcca atggcggtga tttctgagat
     2941 ccccaccgcc atccacataa accagtaaga ccaggcggtg agatagccaa aaaacgggct
     3001 catataacga tgcgcataaa cggcgaacga accggtaacc ggttcgagga acaacatttc
     3061 gcccattgaa cgcatgatga aaaaaacgaa cagcccggcg atgatatagg ccaacaatac
     3121 ggatggcccg gcccatttca gggtactggc ggcccccata aacaggccga cgccaatggt
     3181 gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta gctctggttt
     3241 gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt
     3301 tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc aaataaagca
     3361 ttaaaaattt gaatgctttg tgtaataaaa aagcagacag gcgacgaagt gaccactccg
     3421 tcgctttaca aagagaggaa aatcagaggt tgccggtgta gtgccagcgt aaataacgca
     3481 gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag agccactcca
     3541 gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag
     3601 tcccgccaac gcccatatac agcgcatctg gatgcaccag acggcagtcg cgcatgaaga
     3661 tctcctgctt tggcgatccc atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc
     3721 gttcaaacag cgcctgacgc tgctcgggtt taaaataacc atcctgactg ccaacgatat
     3781 tcacattcca ttggttgcgc agtttagctt cagtttgcgc cagcacttca ggtttaccgc
     3841 ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc catcagctct tcccagagat
     3901 cggcaccggc aacgcgggaa acctgcgcct gcgggtactt tttacgtact gaacgtacaa
     3961 tgctgatgcc atccgcatat ttaaattcgg cagcgttaat taactccctg acctcggcgt
     4021 tatcttcaat ggtcagcatt ttttcggcat taatggcaac cagcgttccc tgcttaagct
     4081 gcccgtcagc aaacagataa tcgagggcat gctgcatatc acgccaacca atcaactgta
     4141 agccacgcag cgtataggtc ggtgccgtgg tgttgttatt cattgttatc cttcaacctg
     4201 cgtccggagc gatgattttg tacgtttatg aatgagtccg gcgctttcaa aaagccagta
     4261 caacagtttt gcgatcatca gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga
     4321 gacaaacgaa tccagccctt cacgtgccag cacgatcata ttgaagatcg ccccaaagca
     4381 gaaactgtgc aatatcgcag ccttatagcg attagtctcg cggttgccca gctcatacag
     4441 ccagtcgaac catttgatga tcagtccaac cacaatcgcc ccgagtggga tgaacaacgc
     4501 gccgcccatc accaccagtg agcctataag cgtaggcgag atcgccagtc cggagtggtt
     4561 attcagtact tcccaggtaa agtaattggc tgagttcagc accatactcg ggcgccccgg
     4621 ccacagccag gaagggataa agacatagaa atcgcggaca attggagcca ggccctggaa
     4681 gtcgatgttg tcgtagttct gcaacagcaa cgccagattc tcccacggtg aaaaggtgtc
     4741 gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg ctcacattca ttccatagcg
     4801 ttttaacgcc agccagaaca tgccaacaat ccccagcacg cccgccgccg ccagcatcca
     4861 caacgaaatc cagccgcgaa taatgccaat aaacaggaag atagcgaatg cgatgatgat
     4921 attggcgcga gtgccgccga caatcatata agtcagcaag ccaaaggcga ccgtgctgac
     4981 gaggaaaaac agccacgctt tgctgtcctg gcgcagaaag tagaccacca gcatcgccgg
     5041 gatgaaaaag taaaagaagc gttttaacgc cacgccggag acttcactgg aaaagatctg
     5101 actgtaggag ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct
     5161 taccagcgcg atacccatca ggatcaccca cgtaagattg gtctccacgc ggttcatggt
     5221 aaacagcgga cggcgcggta catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac
     5281 atagtaaacc gcgtagaagc agcccgcaga aagcaacacc tgcaacaaga tttctggcgg
     5341 cgcgacacca acatcaaagc gaaataccag cacgctggtc agcgggaagc cgaagaaaaa
     5401 ggtgagcaaa aacagcaatg aaaagaagac attgaagtta aagcgcacac ggcgaaactc
     5461 aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc cagaccacaa acaggccact
     5521 gaattgcagc agactcatgc gacctccccg gcggcaatcg ccaacgcccg ctgccagcct
     5581 tgtagatagt tagggctaaa gaaggcaatg gtgtttttat ccgccgacgc caactggcgc
     5641 tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag taaacagcac tggcaaatgc
     5701 tgttccgtca tatcctgcca gaacggattt tccctgttaa gcacacaagg aatgcccgcc
     5761 tgaatcagta agcaaagcgt accaatgccc tgctggcggg caaaaataaa gtaaccaaga
     5821 tcgcactgac gaagtagcgc cagataggcg tcaaattcca gtttttcgct caaaatttgc
     5881 agattttctt cgctgaataa ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg
     5941 ttattaggcg gatatcccat cggcaccacc acttttaccg tatcgccaaa ttgctgatga
     6001 acggcacgta aagcggcgac atgctcattg ctgcggtcgc cggagttccc caccagaatg
     6061 gtcatttttc cttcacgttg ccgatcgttc gccatcgtat tgagagaagg gtccatccgc
     6121 gtcgggaagt acagcagttc tccccgcacc tttgggtgtg ttttggcaaa aaagctcaaa
     6181 tcgccgcggg tggcaaatac acagccgact cgcttttgcg ccaggcgacg tagtgggtaa
     6241 aaaagcttat atctcaagcc actggaaagc tcatacaggt ctgcccccca gatatgccag
     6301 aaaaactggc tgggcttaat gccaccactc agcagagcca gccacagtgt gggattgaac
     6361 tgaccgtgga aaaaaaatcg ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc
     6421 gccagcgatt ttttcccagg gaaaaattgc acagaaagcg ccggacagct atcacttaag
     6481 ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc ggccagcgcg
     6541 tcattgaaaa accgcaaaac ggttcggtta tggtgaggga tatccgatcc cagtacgtga
     6601 atcagtacag tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa agagaaaaat
     6661 agacgatata agttgccata tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta
     6721 gccagtgggc aaataccatc aataaagtga actggctgac ttccgccaga atataaaacc
     6781 gcagtgacgc tttggcgatc accagataac caaagacata agcgcccact tttaacacat
     6841 cacccactaa ctgccaggca aagagatcgc gcatagcggt aaatttattc gacaacagca
     6901 accagatagc aaaatcacgc agcaaccaga cggtaaaact cgctgccgcc actgccggta
     6961 agacgaattt cagcgattta accacttccc gggtgatatc gcgcttttcc gttagccgcg
     7021 acaacgtggg cagcaaatat acgctgaacg atgccgtaat aaattgcagg taggcatcgg
     7081 aaatactgct caccccttgc cagatcccca cttcatccca gctatactgc gccgccagca
     7141 gtttacgcat catgatgtaa gcaacaggca aggtcaccga cgtaatcaac gccatgagcg
     7201 taaatttgct caactgccct gccagaccgt tatcccagct gggtttcaga tagcttaacg
     7261 ggatgacacc acgtttaatc aacatgatgg cggcaggaat taccaccagc gcgggaatca
     7321 gcgccagacc cagcaacgcc ccttcataac cgcccaaacg gtaactgacg tagtacgcga
     7381 gaacgccaat caagctgccg acaatcagcg ataacgcatt acctgcggca tcgcgaaagc
     7441 ctttcatcag cgccagtaac aggttgcccc aggcgatccc catttgcacc agcgccacta
     7501 aacgcaccag cccctgatag tcggtattac caaacaatcc ctggctgatt ggcgcagctg
     7561 ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag taccatcgct gatgaagtgc
     7621 cgaccacgcg gcgcagctgt tgcggattat catggtactg ggcaacgtat ttggttacac
     7681 cgttaaagat gccagccccg gcaagcacgc cgagcacggt aatcaactgg cggaaatttg
     7741 ccgccagccc aagccccgcc ggaccaaatg acaccgccag caacttaccg accagtaacc
     7801 cggcaccaat cttgaccagt gtactggccg ccgtccacaa ggacgctttt gccaacgaca
     7861 tatcaggaga agtagttcaa caaagtcgca attaccgtac gctgattgac gggcgacagg
     7921 ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt tggtggtgta gcgatcttca
     7981 ccgtggaact caccaaagtg ttcccccgca gggcaaccgt gcagcggaat gtaatgaaac
     8041 accgccatga tttccgcttc tttcagaaag ttaatcaacg cgctccggtc atcaatatcc
     8101 cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc catcgggaat cgacggcagc
     8161 tcgatacgcc cggctttcgc cagaggcgct aacgtatcgt agtagttttg ccacagcgcc
     8221 agacgttgct ggttgatacg atccgctgct tccagttgcg cccacaggta cgcagcttgc
     8281 agatcggaca tcaaatagct ggagccaata tcgcgccagg tatatttatc gacctgacca
     8341 cggaagaact ggctgcggtt agtgcccttt tcacggatga tctcggctcg ttcgattaac
     8401 gctttatcgt taatcagcgt cgcgccgcct tcaccacccg ccgtgtagtt tttggtttca
     8461 tggaagctaa agcagccaat atgaccaatg gttcccagtg cacgcccttt gtaagtggac
     8521 atcacgccct gagcggcatc ttccaccaca aacaaattat gctttttcgc caacgccata
     8581 atggtgtcca tttcgcaggc cacacccgcg taatggaccg gcacgataac gcgcgttttg
     8641 tcggtgatcg ctgcttcaat caacgtttcg tcgatgttca tggtgtccgg gcgaacatcc
     8701 acaaagacga ttttcgcgcc acgcagcaca aaggcattgg cggtggagac aaaggtgtag
     8761 ctcggcatga tcacttcatc gccaggctgg atatcgagca gcagcgccgc catctccagc
     8821 gaagcggtgc aggacggcgt cagtaacact ttggcgctgc caaaacgttg ctccagccac
     8881 tgctggcagc gacgggtaaa accgccatcg ccacacagtt tgccgctacc cattgccgac
     8941 tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt taaatggaat catgtgatca
     9001 cctgtataac cagtacgcgg tgctttctac attcgcacca ctttgtatgt atcgtttaag
     9061 cgcggcggtg ttgcccattt gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc
     9121 ccagtttagc gccgtttgca tcagctcagc acctgcaccg cgtccagcca gcaggccaat
     9181 tcgcgcatct gtcgcattga gttcccgtaa agagacatag ccgcgaatat cgccggacgc
     9241 cgcacgtaga atcagacatt gatgatcaaa ggtgccgcgc acggcatttt caatccactg
     9301 tgcataaaag cgaccgctgg cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc
     9361 aaatgcggcg ctagctaact gacgtaatgc gggaatatcg gtctcttgtg ccacgacagc
     9421 accgctatca ctgacattgt tcacgggtag cgccaaatca acttcacctt ctaccaggga
     9481 gaatcccagc tgttgcaggg catccagttc ccccgtattt gatgccgcaa ttttggcctg
     9541 cacccgtgac catggcgcta aggcgtctga cgtcaggagc ggtgcttcag acgtaatgcg
     9601 cacgatggcg ctgttaacac caaagaaggc gttttcccag gttagtggct caatactggc
     9661 gcggacgggc acgaagtaac tccagcagat attggccgta gccagttttc gctaatgaac
     9721 tggcagcacg cttcacaccc tcgtcatcga gccagccgtt acgccaggcg atctcttcca
     9781 ggcaggcaat cttaaagccc tggcgttttt ctactgtctg cacaaaagtg ctggcctcaa
     9841 tcaggctgtc gtgagtgccg gtatccagcc aggcaaatcc gcgcccgagc agttcaacgg
     9901 tcaggttgcc cgcctcgagg tacatctggt tgatggaggt aatctccagt tcaccgcgct
     9961 ccgacggctt cacctgcttt gcgtactcca cgactttact gtcgtagaaa taaagcccgg
    10021 tcaccgccca gtttgacttc ggctgttttg gcttttcttc aagcgagata gcgcggaaat
    10081 tgtcgtcaaa ttccaccacg ccaaagcgtt ccgggtccat cacctgatag ccaaaaaccg
    10141 tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc tgaccgaaga
    10201 agatgttatc gcccagcacc agacaagaag gttcaccatt aaggaaggtt tcaccgatga
    10261 taaaggcctg cgccagaccg tccgggctgg gctgttcggc atattccagc tggataccga
    10321 actcactacc atcgcccagc aggcgctgga aataaccttt atcttccggc gtagtgatga
    10381 tgagaatttc gcggataccg gccagcatca gcaccgacag cggatagtaa atcattggct
    10441 tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc aatcgggtac
    10501 cggaaccgcc cgccaggata atacccttca tgtttgcctc cgctgaaaat tagcctttca
    10561 gacctaaacg ctcaccctga tagctgccgt cctgcacctg cttccaccag ctttcattag
    10621 ccagatacca ctgaaccgtt ttacgcattc cactttcaaa ggtttcctgc ggcagccagc
    10681 caagttcacg ggcaattttc gaagcatcaa tggcataacg cagatcatgc cccggacggt
    10741 cagcgacaaa ggtgatcagg tcacgatagt acgccacgcc gtgcggcttg ttcgaagcca
    10801 gttcttccag cagctcgcaa atggtttcca caacatcgag attcttacgc tcgttgtgac
    10861 caccaatatt ataggtttca ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg
    10921 cgtgatcttc cacatacagc cagtcacgga tttgctgccc gttgccatat accggcagcg
    10981 gtttacccgc cagcgcgttg aggatcatca gcgggatcag tttttccgga aagtggtaag
    11041 gaccgtagtt attcgagcag ttggtgatca gcgtcggcag accgtaggtc cgcagccagg
    11101 cgcgcaccag atggtcgctg ctggcttttg acgcggaata agggctgctc ggcgcatacg
    11161 gcgtggtttc ggtgaagaaa tcatccgtcg agtgcaggtc accatatact tcgtcggtgg
    11221 agatatgatg aaaacggaac gctgattttt tatcttccgt cagcgtattc cagtaagccc
    11281 gcgccgcttc aagcaatgta taagtcccga caatgttggt ttcaataaac gctgccgggc
    11341 cgtcaataga acggtcaaca tggctttcgg ctgccagatg catgacacag tctggctgat
    11401 gctcagtgaa tacgcgtgcc agttctgccc gatcgcagat atcaactttc tcaaaggcaa
    11461 agcgctcgct ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag gtcagcttat
    11521 cgaccactac caccgcatcg ctcgtttcgt tgatgatata acgcaccagc gccgagccaa
    11581 taaacccggc accacctgtt atcagaattt ttctcatcag cgccagactc ctttggcatc
    11641 gacgacatac tgctgatgga cattgtcgcc attgataact ttgaactgac tatgatcgac
    11701 cagcatcacc agcacgtctg ccgttgccag cgcctcgtca agctgcgcca gagtacaaag
    11761 cccggtcagt tttttcggca actggtggat gttaggctca acaaccagcg tttcgccgct
    11821 gtgccactgg gcgatcagtt cagcgatttc catcgccggg ctttcgcgca ggtcatcaat
    11881 attcggttta aacgccagac caaagcaggc gattttcagt tcactggctc gtttatcggt
    11941 agcagcaagg cagtcggcaa ccgccgcttt cacctgatcg ataacccaga acggtttgtg
    12001 atcgttaact tcgcgcgcgg tacggataag ccgcgcctgc tgggggttct gtgccacgat
    12061 aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca gggccaggct gaagaatatt
    12121 aacgcgaggg tgacgattcg ccaggcgaat cagttcccag acgttaatcc cctgatcggc
    12181 acaaatcagc gacaattcat tagcaaaagc gatattcaca tcgcggaagc tgttttcggt
    12241 gagcttacac atttccgccg tccgcgagtt agtgacgaca cactcacctt cgaggaaaat
    12301 tttgtacaat tcgctggcgc gggccgaaca aaccggcgtc ataccaccaa tcacgcgatc
    12361 gtttttaatc agctcgacca taacctggcc cggtaataca cgttccgggc agtaagcaat
    12421 gttgacgtcc gcctgctcgc ccacctgctg tgggaaagtc agatccgggc gcatctctgc
    12481 taaccattct gccatcttct cggttgaccc caccggcgag gtggattcaa ggatcaccag
    12541 cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc
    12601 tggctcatga tcgcctttaa acggcgtagg tacagcaatc agccaggcat ccgcttcaac
    12661 tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca gtttttacta cgctcgccaa
    12721 atcaggctcg acgatatgga tttcgccacg attgatggta tcaaccgcgt gttggttgat
    12781 atcgacacca atcacctgtt tttgccgtga ggcaaacgct gctgctgtag gcagcccgat
    12841 gtaacccagt ccgataacag aaatggtcgc aaaactcata gtgatatccg attatttttt
    12901 aacgcttcca gaatgcgaga gcatgcctga ccatcaccat acgggttatg ggcgcggctc
    12961 atagcttgat attcgttttc gtcttttaaa agacgcgtca cttcctcgac aattcgctgc
    13021 ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg cttccggacg ctcagtggta
    13081 tcgcgcatca ccagcaccgg tttccccagc gaaggcgctt cttcctgaat gccgcctgag
    13141 tcagtcaaaa tcagccaggc gtggttcatc agccagacaa acggtaaata ctcctgggga
    13201 tcgatcagaa tgacattttt cacatgcccc agaatgcgat tgaccggctc tctgacgttc
    13261 gggttgagat gcaccggata gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc
    13321 agcgcgtggc agatttcttc aaagccacga ccgaaactct cacgcctatg accggtcacc
    13381 agaatcatct ttttatcggg ggcgataaac gggtaatttg ccgccagttc tgaacgcagc
    13441 gtgtcgctgc tcatcacctg gtcacgcacc cataacagtg catcaatgac tgtattaccg
    13501 gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca agttttgccg ggaagtttcg
    13561 gttggagaga agtgatacat cgccagatgc ccggtcaatg tacggttagc ctcttccggc
    13621 cacggcgaat agagagcgcc cgtgcgcaga ccagcctcaa cgtgaccaac aggaatacgc
    13681 tgataaaacg ccgccaggct ggttgccagc gtcgtcgtcg tatcgccgtg aaccagcacg
    13741 acgtctggtt tgaactcggc aagaataggt tttagccctt ccagaatccg acaggttatc
    13801 tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac aatggaaaag
    13861 agtttcagta cctgatcaag catctcccga tgctgcgcag tgacgcaaac tttagcctca
    13921 aaaaaaggat cttttgccaa cgcatgcacc aacggagcca tcttgatggc ttccgggcgc
    13981 gtaccaaata cagtcagtac tttcacatcg attctcttcg aataagcggc gagcgccttt
    14041 gcgctcaccg cagcagtgtt gctatttcga gcaacggcgg gttaatgcga caccagcccc
    14101 gatcagcccc ccgacaatgc cccacataat catcaggaag gcacgacgtg ggctatcgcg
    14161 ttttaccggt tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg gatccagggt
    14221 tggaccaaca ttcagggtgt ttaacatggc tcgattctga tcatagtcga gatcaaaggc
    14281 cggaccgacg gcctgtaaat tttccagtcg agcctggagc attggacgcc caagcaggaa
    14341 catttctgaa tcaggtaatt cctcggcagg cacatctgtc gcactgcgcg aaatattatg
    14401 ctgctcagca attttcagcg cctgctcaat actgttcatc cggcggtcgt agatggcttt
    14461 cgccacctct tcctgacgct tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc
    14521 gcctttcagc tcatcattca gatggctggc tgcacgctgg ctggcaaaag caacatactg
    14581 acgtaacagg ttattagcgt caggcgcggt ttcggcaata agcttcacgc tgtcattgac
    14641 cgcgcgagta aagtctccgg ggataaactg gatgttgtta atcatttcat ccagcaacgc
    14701 cgcatcggct ttgctgttgc ccaccatccg ctgtttgtaa tagtcggttt gcagccagaa
    14761 ctctctgcgg gtatcccacg aggccagttg cataacaaac tctttatagg cttcgtccat
    14821 gaccgatggt tgatcggcag aagccatgtt tgaacggaca tccaggttac gcaaaaattg
    14881 ctgctgcgag taatatcccc ccagcatatt caccgttgga cgatcggtaa tcgccgtcga
    14941 gctccactcc tgacgagcaa aaaaagtata cgccagcgcg attaacgcaa acgccagccc
    15001 catgccaata atccatagct tcccagccca caaggtacga aacaacccac gaatatccag
    15061 ttcattttca gcgtcttcgg ccggtttccc aggcattggt tgtgtcatca catcctcatt
    15121 tatttggtta aattggggct gccaccacga tttctacgca gtctgcgttt tacgcgctta
    15181 ataaagcgag caactttcca ggcacgctta atgcaatatc catagaggaa gaatgctagc
    15241 aaaaagagca ccagcatgac ccactccggg acaaaatgag aatattctgc cagcacgcca
    15301 atggaagcga gcagtgctgc ggcaagggta atcagcacaa acgcctgacg ggaagtaaac
    15361 ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag gagagaatgg gctcatgcct
    15421 ttacgcaggc gacggtacat aatcgccacc atatccatta gcggaatggc gattatccac
    15481 aaagcggtaa ccgggctgat gggatgggtt ttgccctggg tcgtttcgag caggatccag
    15541 ataacggtaa aaccaatcag cgtactgccc gcatcaccca taaagacttt gtagcggcga
    15601 cccaggatac caaggttaag catgatgtat ggcaggatgg cggcgatcat cgcaaagcac
    15661 cagattgcga ggctggtttg cccgtcgaac cacaaaatca taccgattgc tgcaaacgag
    15721 acgcaggaca acccgcccag caagccatca atgccatcaa ccatgttgaa cgcattaatg
    15781 gccgcccaga cggcaaatag cgtcaggaag taaccaaacg gtccgagcac catctcccag
    15841 gagccaaaga tataacccag gctactgaga taaagtttgc cgaacaccat cataacaatg
    15901 ccaacagcgg cctgtatggt ggcacggatt tttacgctga tatcaaaacg atcatccagc
    15961 gcgccaatga aaacaagcac accggcacaa gcgagataga gagatgcatg cggaatatag
    16021 taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga
    16081 ggtatcaatc cctggtgacg tttgcggaag tttggtttat ccactaaacc gacttttttt
    16141 gccaccttac gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact gatgagatca
    16201 gtactcactg tcagtaaatt cacaatgcat gctctcagag aaaattatta gcagaagtat
    16261 aaccacgaag acctttattc agaagggaaa gtccgttccg aacccaccag ccctgtgcaa
    16321 aaatcaacca tttgatatgc aaattgcata acatgccagc aaattccagg aattttccta
    16381 acatcgccag cgcgacatta agattacaga tataaaagca aaacgccacg taaacacgtg
    16441 gcgtttttgg cataagacaa atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg
    16501 tcttggtcat ggccagttta ttaatgagga attccattgc atcgatttcg cccatcgggt
    16561 gaatgatttt gcgcaggatc cacattttct gcagttcttc ctgagtcgtg agcagctctt
    16621 ctttacgggt accggaacgg ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct
    16681 tacgagagag gtgcagttcc atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt
    16741 ccattttaga accggtatcg ataagcgccg tcgcgataat ggtcaggctg ccgccctctt
    16801 ccacgttacg cgccgcacca aagaagcgtt tcggacgatg aagggcattg gcgtccacac
    16861 caccggtcaa cactttacct gacgccggaa caacagtgtt gtaagcgcgc gccagacgag
    16921 tgatggagtc gagcagaatg ataacgtctt tcttgtgctc aaccaggcgt ttggccttct
    16981 cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa
    17041 caacttcacc ttttaccaga cgctgcatct cggttacttc ttccggacgt tcgtcgatca
    17101 gcagaaccat cagcacacaa tccgggtggt tgtaagcaat gctctgagca atgttctgca
    17161 gcagcatagt tttaccggct ttcggcggtg ccacaatcag accacgctga ccacgaccga
    17221 taggtgatgc cagatccagt acgcgagcag ttaaatcttc agtagaaccg ttaccacgtt
    17281 ccatacgcag acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc
    17341 gggcgttttc aggtttgtcg aagttaactt cgttaacttt cagcagcgca aaatagcgtt
    17401 caccttcttt cggcgggcga atcttaccag agatggtatc accagtgcgg aggttgaaac
    17461 ggcggatttg gctaggggaa acgtagatgt catcaggacc ggcgaggtag gagctgtctg
    17521 cggaacggag gaaaccaaat ccatcctgca atatctccag tacgccatca ccaaagatat
    17581 cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc ttacgcatgc
    17641 gagccaggtt ttccagcccc atattttcgc cgagagtgat cagctcagaa accggcgtat
    17701 tctttaattc ggtaagattc atagtggtgt gagttcttaa acttggggta aatctcgaac
    17761 ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt ttacggtcat cgactcatgt
    17821 cttttcgctg cctggtcaca ggagagtacg cagaactgaa acgacaagac ggattgagtg
    17881 acgagccaga aatctgttca cttcgcattt aagataaaac gggaagcgtt ggaaattaca
    17941 agattcaaac ttaataaggt atgtttaata cgaagtcaac actaagttag catgactcac
    18001 gccgggcgtc cagtttttag cgacggggca cccgaacatg aaattccctt acgccaggtt
    18061 agcgtcgagg aactctttca actgaccttt agacagtgcg cccactttgg ttgccgccac
    18121 ttcaccgttt ttgaacagca gcagagtcgg gataccacgg atgccatatt tcggcgcagt
    18181 gccagggttt tgatcgatgt tcagttttgc aacggtcagt ttgccctgat attcgtcagc
    18241 gatttcatcc agaatcgggg cgatcatttt gcacggaccg caccactctg cccagaaatc
    18301 gacgaggatc gccccgtccg ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg
    18361 aataatttta tcgctcatat ataactccac aggaataagc ctggcgtgtt ggtttcgttg
    18421 ttggtgtaac attaaccaac taaaggttga ctttatttca ccggatacgc tttcgtaaag
    18481 caatagtaag ctgatattct accacactat gagcaaaaca catttaacag aacagaagtt
    18541 ttccgacttc gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa
    18601 ctgtacgccc attcaggcac tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg
    18661 gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct
    18721 tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa ccgcgtgcct taattatggc
    18781 accgacgcgt gaacttgccg tgcagatcca cgccgacgcg gaaccgctgg cggaagctac
    18841 cggcctgaag ctgggcctgg cttacggtgg tgatggctat gacaaacagc tgaaagtgct
    18901 ggaaagcggc gttgacattc tgattggcac cacgggtcgt ttaattgact acgccaagca
    18961 gaaccacatt aacctcggtg ccattcaggt agtggtactg gacgaagccg atcgcatgta
    19021 cgatctaggc tttattaaag atatccgctg gctgttccgc cgtatgccgc ctgcaaatca
    19081 gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga
    19141 gcagatgaac aatgccgaat atattgaagt ggaaccggaa cagaaaacgg gccaccgtat
    19201 taaagaagag cttttctacc cttctaacga agaaaaaatg cgtttgctgc aaacgctgat
    19261 cgaagaagag tggccagaca gagcgattat tttcgccaat accaaacacc gttgtgaaga
    19321 gatctggggc cacctggcgg cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc
    19381 gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt ggcgatctgg atattctggt
    19441 tgccaccgac gttgccgcgc gtggtttgca tattccggca gtgacgcacg tctttaacta
    19501 cgatttaccc gatgactgtg aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg
    19561 cgcaagcggt cactctatca gcctggcgtg tgaagagtat gcattgaatt tgcctgctat
    19621 tgagacctat attggtcact caattccggt aagcaaatac aatccggacg cattgatgac
    19681 cgatctgcca aaaccgctgc gcctcacgcg cccgcgcaca ggcaatggtc cgcgtcgtac
    19741 tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc
    19801 aaactgtatc aagatggcaa gagaatgaat cccgatgagc ttactgtagt aagtgattcg
    19861 ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg ggttccacct
    19921 cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg
    19981 aggtggctgg aagcatccag acgctgacgc gaattaaacg caaagtgcgt ctggctgctg
    20041 gcctgaatag cgaaaatgac ctctctaatg aagcaatgga gcgcggttgg caatgtctgc
    20101 gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg
    20161 cgacattacg acttgccgtc aatgcgggtg attttattgc caaagcgcag gaaatcctcg
    20221 gttgtccggt acaggtgatc agcggtgaag aggaagcgcg tctgatttat cagggcgttg
    20281 ctcacaccac tggtggtgcc gatcagcgcc tggtggtgga tataggcggt gccagtactg
    20341 aactggtaac cggcacgggt gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg
    20401 tcacctggct ggaacgctat tttgccgatc gtaatctggg gcaagaaaat tttgatgctg
    20461 cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct
    20521 ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac
    20581 aggggatgga tgaacgcatt accctggaaa agttgcagca attgaaacag cgagccattc
    20641 attgcggtcg gctggaggaa ctggagattg acgggctgac gctggaacgt gcattagtgt
    20701 tcccgagtgg tctggcgatc ctgatcgcca tttttaccga actgaatatt cagtgtatga
    20761 ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg
    20821 agcaggatat tcgcagccgt acactgcgta atattcaacg ccgctttatg atcgatattg
    20881 atcaggcaca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg gaaaacgaat
    20941 ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg
    21001 gcctgagcgt tgacttcaaa caagctccgc aacacgctgc ttatctggtg cgtaacctgg
    21061 atcttcccgg ttttaccccc gcacagaaga aactgctggc gacgctactg ctcaaccaga
    21121 ctaacccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccaccg cgcgtcgcag
    21181 aacaactctg ccgtttactc cgcctggcta tcatttttgc cagccgtcgc cgtgacgatc
    21241 tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag
    21301 gttggctaac ccaacatccg ctgggtaaag agattattgc tcaggaaaac cagtggcaga
    21361 gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgacg
    21421 catcttctcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc
    21481 cagatggctt tgccctttct gcatccgttc ttcggcactg accactttgc gctcctgttc
    21541 ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg ctcggctccg ggcgcaccag
    21601 ttcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt
    21661 aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga tactgctctg
    21721 gtgcggcaaa aacccttctt ccataccgac catgtagaca taaggaaact ccagcccttt
    21781 tgacgcgtgg agagtcatca gttgcacctg atccagctct tcttcactct caccacgctc
    21841 catcatgtcg cgtaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag
    21901 ttcgctgcct tccagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg
    21961 catttcggca gctttcgtgc tgggcgatgt ttcgtacagc caggattcat aatccatgcc
    22021 gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttctgc
    22081 caaccagtga gtgaagcggg tcaatgcttc ataaccacgt ccgctaagcg tctgactcag
    22141 gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc actcacccag
    22201 ctttttcagc gtcgccgggc caatctctcg cttcggcgtg ttaacgatac gcagaaatgc
    22261 gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg
    22321 acgagagaaa aacgacgtac caccagaaat tttgtacggg atgcggtttt gcatcaggaa
    22381 tttttcaaac acccgcgact gatagttacc gcgataaaga atagcgtaat ctttgtactg
    22441 cgttttattg acgaagtgat gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc
    22501 ttcgttattc gcgcttaaaa cttttagctc tgtgccgtaa cccagttcgg agaacagacg
    22561 cttttcaaag acgtgcgggt tattggcgat caggatgttc gccgctttca aaatacgtcc
    22621 ggaagagcgg tagttctgct caagcttaat caccttcagc gccggaaaat cctggctcag
    22681 cagcaccagg ttttgcggac gtgcgccgcg ccaggagtag atcgactggt catcgtcacc
    22741 caccacggta aagcgcgcgc ggctgcccac cagcagtttc accagttcat actggctggt
    22801 gttggtgtcc tgatattcgt ccaccagcag gtagcgaatt ttgttctgcc agcgctcgcg
    22861 gacttcttca ttgcgttgca gaagtagcgt cggcaataaa atcagatcat cgaagtcgag
    22921 cacattacag gctttcaggt gtgcatcata cagcccataa caatgggcaa aaatacggtc
    22981 ccgctcgcca atcgcactgg ctgccgcctg ggacggtgtt ttgagatcgt tcttccagtt
    23041 agagatggtc gaaatcaact gttgcaggag aactttgtca tcttcaatca gcccttcggt
    23101 caactctttg agcaacgcca gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat
    23161 ccccagcgcc gcatactcac gcttgatgat atccagcccc agcgtgtgga aggttgagat
    23221 catcagccca tgcgcctctt tgcgacccag cgtctgtccg acacgctctt tcatctcgcg
    23281 cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac cgcacccgcg
    23341 gatcagatgg gcgattttat tggtgataac acgagtttta ccggaacccg cacccgccag
    23401 caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt ttagacgcat
    23461 aggaaaatcg ctcaatgaaa gtcgggaggg gaaaataaca gcgtggtagt atagccagcc
    23521 tcatccttac cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
    23581 taaaagaaga gaaactggct ctggatcttc tcgtgcagat taagaacggt gccgatttcg
    23641 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
    23701 aattccgcca gggtcagatg gttccggcgt tcgataaagt tgtgttctct tgtccggtac
    23761 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
    23821 gtaactaata gcaaggcctt ctccgggaga aggccttgaa ctctgaatcg ctattaaccc
    23881 gcaacagcaa tacgtttcat atccgtcata tagccgcgca gtttcttacc tacttgctca
    23941 atcgcatggc tgcgaatcgc ttcatttaca tcacgcagct gcgcgttatc taccgcacct
    24001 tccggaatag ctttacccaa atcgcccggt tgcagttctg ccataaacgg tttcagcagc
    24061 ggcacacaag cgtaagagaa cagatagtta ccgtactcgg cagtatcaga gataaccacg
    24121 ttcatttcat acagacgctt acggacgatg gtgttggcaa tcagcggcag ttcgtgcagt
    24181 gattcatagt aagcagattc ttcgatgatg ccggaatcaa ccatggtttc gaacgccagt
    24241 tcaacgcccg ctttcaccat cgcgatcatc agtacgcctt tatcgaagta ctcctgctcg
    24301 ccgattttgc cttcatactg cggcgcggtt tcgaaggcgg ttttgccggt ctcttcacgc
    24361 caggtcagca gtttcttatc atcgttggcc cagtccgcca tcataccgga ggagaattcg
    24421 ccggagatga tgtcgtccat atgtttctgg aacagcggtg ccatgatctc tttcagctgt
    24481 tcagacaacg cgtaagcacg cagtttcgcc gggttagaga gacggtccat catcagtgtg
    24541 ataccgccct gcttcagcgc ttcggtgatg gtttcccaac cgaactgaat tagtttttct
    24601 gcgtatgccg gatcggtgcc ctcttccacc agcttgtcga agcacagcag cgagccagcc
    24661 tgcaacatac cgcacaggat ggtttgctca cccatcaggt cagatttcac ttccgcaacg
    24721 aaggacgatt ccagcacgcc cgcacggtga ccaccggttg ctgccgccca tgctttagcg
    24781 atcgccatgc cttcgccttt cggatcgttt tccgggtgaa cggcaatcag cgtcggtacg
    24841 ccgaatccac gtttgtactc ttcacgcact tcggtgccag ggcatttcgg cgcaaccatg
    24901 actacggtga tgtctttacg gatctgctcg cccacttcga cgatgttgaa accgtgcgag
    24961 taacccagcg ccgcgccgtc tttcatcagt ggctgtacgg tgcgcactac atcagagtgc
    25021 tgcttgtccg gcgtcaggtt aaccaccaga tccgcctgtg ggatcagttc ttcgtaagta
    25081 cccactttaa aaccattttc ggtcgcttta cgccaggacg cgcgcttctc ggcaatcgct
    25141 tctttacgca gagcgtagga gatatcgaga ccagaatcac gcatgttcag accctggttc
    25201 agaccttgtg cgccacagcc gacgatgact acttttttac cctgaaggta gctcgcgcca
    25261 tcggcgaatt catcgcgccc cataaagcga catttgccca gctgtgccag ctgctgacgc
    25321 agattcagtg tattgaagta gttagccatg gtgattcctc gtgatgttgt gcttcttatt
    25381 gttcggttcg cgtttgcgag attgaattca ctatatgaca ggaaatttat tgcggaaatt
    25441 gatatattca caacgtcaca ttgcaatttc tgcaacgtca acattgaggg ctgcgccatt
    25501 ggatttacgc gatctgaaaa ccttcctgca tctggcggaa agccgccatt ttggccgcag
    25561 cgcgcgggcg atgcacgtta gcccgtccac gctctcacgg cagattcagc gcctggaaga
    25621 agatctcggc cagccgctgt ttgtgcgcga taaccgcacg gtgacgctga ctgaagcggg
    25681 cgaagaactg cgcgttttcg cccagcaaac gctgttgcag tatcagcagc tgcgccacac
    25741 catcgaccag caagggccgt tgctctatgg cgaattacat atcttctgct cggtgaccgc
    25801 cgcttacagc catctgccgc cgattctgga tcgcttccgc gcggaacacc cgtcggtgga
    25861 gatcaaactc actaccggtg atgcggcaga tgcaatggaa aaagtcgtca caggtgaagc
    25921 tgatctggcg attgccggga agccagagac tctgcccggc gcggtggcgt tttcgatgtt
    25981 ggagaatctg gcagtggtgt tgattgcacc cgcattgccc tgcccggtgc gtaatcaggt
    26041 ttctgtcgaa aagccggact ggtcaacggt accgtttatc atggccaatc aggggccggt
    26101 gcgccgccgc attgagttgt ggttccgacg caataaaatc agtaacccga tgatttacgc
    26161 cacggttggc gggcatgaag cgatggtatc gatggtggcg ctcggctgtg gcgtggcatt
    26221 gttgccggaa gtggtgctgg aaaacagccc cgaaccggtg cgtaaccgcg tgatgatttt
    26281 agagcgcagc gatgagaaaa cgccgtttga gctgggcgtg tgtgcgcaga aaaagcggct
    26341 acatgagccg ctaattgagg cattctggaa gattttgccg aaccattaac ccgccaaaaa
    26401 gaacctgaac gccgggttat tggtttcgtc gtggcaatcg tagcccagct cattcagccg
    26461 ggtttcgaaa tccggttcat ggtcgccaag ttcgaacgcc gccagtacgc gcccgtagtc
    26521 ggtgccatgg ctgcgatagt ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag
    26581 gaagcgcagc agcgcgcccg gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa
    26641 cggatgtgac ggacgcccgc cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc
    26701 ggagagatcc accacgctgt agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc
    26761 ttcgaggccg cggctcaggc gcacgccgac aaagatgcag gcgtttttgg catcggcaaa
    26821 acggtagttg aactcggtga ccgatcgccc gccaagcagt tggcagaatt tgaggaagct
    26881 gcctttttct tccggaatgg tcaccgccaa caacgcttca cgctgttcgc ccagttcgca
    26941 gcgttccgag acgtagcgca ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc
    27001 cagccgttcg ccgcgaatgt tgtgctgggc gatatatttt ttcattcccg ccagcgccag
    27061 cgcgccagag ggttccgcca ccgcacgcac atcttcgaat aaatccttca tcgccgcaca
    27121 gatcgcatcg ctatcgacgg tgatgatgtc gtcgagatac tcctggcata aacggaaggt
    27181 ttcgtcaccg atgcgtttta ccgcgacacc ttcggcgaac agtcccacgc gtggcagatc
    27241 aaccggatga cccgcatcca gcgctgcttt caggcaggcg gagtcttccg cttctacggc
    27301 gatcactttg atttgcggca tcagttgttt gatcagcacc gctacgcccg ccgccagacc
    27361 gccgccgccg actggcacaa atacgcggtc gagatgggcg tcctgctgga gcagttccag
    27421 cgccagcgtg ccttgcccgg caatcaccat cggatggtcg aacggcggca cccaggtgaa
    27481 cccctgctgc tgtgacagtt cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg
    27541 aagcagcact tcgccgccga atccacgtac cgcatcgact ttgatgtcgg cggtggcggt
    27601 tggcataacg atcagggcct tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc
    27661 gtggttaccc gcagaagcag tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc
    27721 catcatggcg tatgcgccgc gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt
    27781 caccagaatg acgttatcaa gacgcgacga cagtttttcc attttttgta gcggcgtaac
    27841 ctgcgccgcc tcgtaaaccg gcgcgcgcag cactgctctt aaatattcgg caccttccgg
    27901 agcaccggac aggggttgcg agtcagccat tattaacccc ccagtttcga tttatcgcgc
    27961 accgcacctt tgtcggcgct ggttgccagg ctggcgtagg cgcacagggc aaaggaaacc
    28021 tgacgttcac ggtttttcgg cgtccaggct ttgtcacccc gagcttcctg cgcttcacga
    28081 cgcgccgcca gttcggcatc gcttacctgt aactgaatgc cacggttcgg gatgtcgata
    28141 gcgatcaggt caccatcttc aatcagacca atgctgccgc cgcttgccgc ttccggtgag
    28201 acgtggccga tggaaagacc agaggtgccg ccagagaaac gaccgtcggt gatcagcgca
    28261 caggctttac cgagacccat tgatttcagg aagctggttg ggtagagcat ttcctgcatg
    28321 ccagggccgc ctttcgggcc ttcatagcgg atcaccacca catcgcccgc gacaactttg
    28381 ccaccgagga tcgcttctac cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg
    28441 aatttgagga tgctgtcatc gacgcccgcg gttttcacga tgcagccgtt ttcagcaaaa
    28501 ttaccgtaga gcaccgccag gccgccgtct ttgctgtagg cgtgttccag cgagcggata
    28561 cagccgtttg cgcgatcgtc atcgagagaa tcccaacggc aatcctgcga gaatgcctgt
    28621 gtagtacgaa tgcccgcagg gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc
    28681 agcataacgt cgtattgttc cagagtttgc ggcaacgtca ggccaagtac gtttttcaca
    28741 tcacggttca gtaaccccgc gcgatccagt tcaccgagaa taccgataac accaccagca
    28801 cggtgaacat cttccatatg gtatttttgg gtgctcggcg caactttgca taactgcggg
    28861 actttacgcg aaagtttgtc gatatcactc atggtgaagt cgatttccgc ttcctgcgcc
    28921 gccgccagca ggtgaagtac cgtgttggta gagccgccca tcgcgatatc cagcgtcatg
    28981 gcattttcaa acgccgcttt gctagcaata ttgcgcggca gtgcactttc gtcgttttgc
    29041 tcgtagtaac gtttggtcaa ttcaacaatg cgtttaccgg cattgaggaa caactgctta
    29101 cggtccgcgt gggttgccag cagcgagccg ttgcccggct gcgacaggcc cagcgcttcg
    29161 gtcaggcagt tcattgagtt agcggtaaac atcccggagc aggagccgca ggtcggacac
    29221 gcggaacgtt caacctgatc gctctgggag tcagaaactt tcgggtctgc gccctggatc
    29281 atcgcatcaa ccagatcgag tttgattatc tgatcggaca gtttggtttt cccggcctcc
    29341 atcgggccgc cggaaacaaa gatcaccgga atattcaggc gcagggaagc catcagcatc
    29401 cccggggtga ttttgtcgca gttagagatg cagaccatgg cgtcggcgca gtgggcgttg
    29461 accatatact caacggaatc agcgatcagt tcgcgagatg gaagtgaata aagcatcccc
    29521 ccgtggccca tggcaatccc atcatccacc gcaatggtgt tgaactcttt ggcaacgccg
    29581 ccagccgctt caatttgttc ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc
    29641 ggtacaaatt gggtgaacga gttcacaacc gcgataatcg gcttgccaaa atcggcgtcg
    29701 gtcattccgg tggcgcgcca cagcgcacga gcacccgcca tattacgacc atgagtggtg
    29761 gtggcggaac ggtacttagg catactttat ttactcccag tgtctgtctc gtaaatggga
    29821 cggtgcgtgc cgtcccattt tttgtattta ttgattaact tgatctaacc agccccattt
    29881 atcttcggtt tcgccagtga agaggccgaa gaaggcttgc tgaatgcgtt tggtaaccgg
    29941 gccacaacgg ccttcgccaa tctgaatacc gtctacgctg cgcactggcg tgatttctgc
    30001 cgccgtaccg gacataaaga cttcatccgc caagtacagg gattcgcgcg acagcacctg
    30061 ctcacgcact tcaattccca gctctttcgc cagtttgatg atggcatcac gggtaatacc
    30121 cggcagcgcg gaggaggtga acggtggggt gaacagcaca ccatctttca cttcaaacag
    30181 gttttcgcct gcgccttcag agatataacc gttcacatcc agcgcgatac cttcctgata
    30241 accgtggcgg cgcgcttcgc tacccaccag cagggaagag agatagttac caccggcttt
    30301 tgccgcagtt gggatggtgt ttggtgctgc gcggttccag gaggaaacca tcgcatcgat
    30361 cccctgctcc agcgcttctg cgcccagata cgctccccac gggaaagcgg cgataatcac
    30421 atcggttgag tatcccgctg gcgggtttac tcccatgcca acatcaccga cgaagatcag
    30481 cggacggata taggcgctgg tgagattgtt tttgcggatc acgtcacgac aagcttccat
    30541 cagctcatca atgctctgtg aaaccgggaa gcgatagatt ttggcggagt catgcagacg
    30601 ctgcatatgc tcacgatggc ggaatacaac cggtcctttg tgtgagtcgt agcaacggat
    30661 gccttcaaaa accgaggtgc catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc
    30721 ttcccagcga accatctccc cattgaacca aatgtaatca gctttcttcg tggtcatttt
    30781 tatattcctt ttgcgctcag gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg
    30841 caacgtgtgc gacgtccacc agtttattta actgactaaa cagtaagtcg accgaccgtg
    30901 ggctggcaac ggtcaattcg atatttatat tttgtgcatc gctggcggca gccatattca
    30961 ttgagcagac gtggaaacca cgatgacgca ccacgcgtaa aacacgttct aaggtttccg
    31021 gattgaagcg agccgataca ttgacctgat gttgcatcat gataatttct ccaacatttc
    31081 tgaattactg gcacctggcg gcaccagcgg ccagacgttc tcaagttcgt cgattgagac
    31141 atgaagcagg tatggcccat cactgttcag catggtgtcg agtgccgctt caacctggtc
    31201 tttacgggtg atgtgttggc cagggatgcc gaaggcgctg gctaacatga ggaaatcggg
    31261 gttatcggta agggtggttt cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg
    31321 aaccatccct aaccgttggt tatcgagtaa gacgattttc aacggtaact gcttgcgttt
    31381 tacggtgccc agctcttgca cattcatcat gaaagagccg tcaccggaga tacagacaac
    31441 ggtatcgttc ggtcgcgcga cttgtgcgcc aactgccgcc ggtaaaccaa aacccatggt
    31501 acctaagccg ctggaggtga tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc
    31561 ccacatctgg tgctgcccca catctgtggt cacgacgcaa tccgcaggtt tacgatccga
    31621 cagttgtttt aacaacaacg gagcgtagat agcgtcaccg ggatggtcgt aacgccagga
    31681 atgttcatca cgcagctgcg cgcagtgttg ctgccagtca ttgatattta acggctgctg
    31741 taatgctggt aacagagcat ttaaatcacc ttgtaatgcc acatgtgcct gacgcagctt
    31801 gttcatttct gccgggtcga tatccatatg gataacactg gcgtgtggtg cgaaggtgtt
    31861 cagtttgccg gtcacccggt catcaaaacg tgcacccacg gcgatcagca ggtcacactc
    31921 ctgcaccgcg aagtttgccg ctttggttcc gtgcatcccc agcatgccca gatagtacgg
    31981 ataatctgct tctactgcgc ccagcccttt cagcgtacag gtggcaggca tttttgtggc
    32041 agcgagaaat tcacgcaaag ccggaactgc ctgcgccatc cccacgccac cgccaacgta
    32101 cagcatcggt ttttgcgctt ttgccagcat ctggcgcgct tgctcgactt cggcatgtgg
    32161 gaaagtcact tcgttttcaa cggtggtgaa ccacggttcc agatcgccgc tggctaactg
    32221 gatatctttt gggatatcga ccagaaccgg accaggacga cctgagctgg caacgtcgaa
    32281 tgcttcagcc atgatgcgcg gcaactcttc cagagactgc accagaaagc tgtgcttggt
    32341 acaggctaac gacaatccca ggacatccac ttcctgaaat gcgtcggtgc cgataaacgg
    32401 tgcggacacc tgaccagtga tggcaacgac agggatggaa tctaacagtg cgtccgcaag
    32461 cccggttatc aggttggttg cgcccggacc agacgtggcg atacatacgc cagttttacc
    32521 ggtagcacgg gcataaccga tagccgccat tgccgcaccc tgctcatgtc ggcacagcaa
    32581 gtgctccacg ccgccgtcat acaatgcatc gtaaaccggc ataattgcgc cacccggata
    32641 accgaaaacg gtgttcacac cctgtgcccg caacgcatgt accacccact gtgcgccatt
    32701 catagttagt tccccgtcct gaatcttgag aaacagaatt ttgtgctgtt attcattgtc
    32761 tgctcctcgg ttatgttttt aaggtcaaaa aaacccccgg acctttcggt gcgggggtct
    32821 tagttcgtta aggcttgatc tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg
    32881 gataataatc accaccacgc taatcacgac caggctaatc actcgtagaa gggctgtcat
    32941 tttgtctttt cttgcatctt gttcgaagga atgcctaaag agttaccata gattttgcga
    33001 atagcacaag atatttttgg gcctattttt atgatggctt taataatttc aatagaaatt
    33061 gttgtttttt aaaaagaaaa tagtgagtga aaaaattcaa tttcagtata aatgttgttc
    33121 ccttcctcgt agttcatcct ttgtccgctg catttttgcg agcatcattc caccgccaga
    33181 acaactcaac ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc
    33241 gggctacagc agcgatatct tatgcacata atggtgactc aaggagggtt tatgtcactg
    33301 tcaattgttc atacccgcgc agccctggaa gtaaatgcgc ccccaatcac tgttgaggta
    33361 catatcagta aaggtctacc cggcttaacg atggtgggct taacagaaac aacggtgaaa
    33421 gaagctcgcg atcgcgtgcg cagcgccatt atcaatagcg gatatgaata tccggcgaaa
    33481 aaaatcacca tcaaccttgc tccagccgat ctgccaaaag aagggggacg atatgattta
    33541 cctatcgcca ttgcgttgct ggcggcctca gaacagctta cagccaataa gttagatgaa
    33601 tatgaattag tcggagaact agcgcttaca ggcgctctgc gtggcgttcc cggtgcaatc
    33661 tccagtgcaa ctgaagctat taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa
    33721 gatgaagtgg ggctaattaa cggtgaagga tgcctgatag ccgatcatct gcaggctgtc
    33781 tgtgcgtttc tggaaggtaa gaacactctc gaacgcccga aaccaactga tgcagtatcc
    33841 cgggcgctac aacatgatct cagtgatgtt gtcggtcagg agcaaggaaa acgaggactg
    33901 gaaattaccg ccgctggtgg gcacaacctt ttactgattg ggccgccggg aacaggtaaa
    33961 acaatgctcg ccagccgtat taatggcctg ttgccagatt taagcaatga agaggcactg
    34021 gagagcgctg cgatattaag tctggtaaat gctgaatcag tacaaaaaca atggcggcag
    34081 cgcccgttcc gctcacctca tcacagtgcg tcgttaactg cgatggtggg cggtggtgca
    34141 attccagggc caggtgaaat ttcgttggcg cataacggcg tgctttttct tgatgagcta
    34201 cctgaatttg aacggcgtac actggatgcc ttgcgagagc cgattgaatc cgggcagatc
    34261 catctttcac gcacacgagc aaaaataacc tatccagccc gtttccagct tgttgcggcg
    34321 atgaatccca gccctaccgg acattatcag ggaaaccata accgctgcac gccagaacag
    34381 acattacgtt atctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    34441 agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca gagagcgcca
    34501 atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac gccaattctg
    34561 caaacttgag agcgaagatg cgatgtggtt ggaagaaacg ctgatccatc tggggttatc
    34621 gattcgtgcc tggcagcggt tactgaaagt tgcacgaacc attgctgata ttgatcagtc
    34681 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    34741 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    34801 cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    34861 gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    34921 tcactggctc acgttcgcca cggcatactg ctacaaagag tttctcttcc tcggtaaccg
    34981 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    35041 cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt ttattgtcga
    35101 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    35161 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    35221 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    35281 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    35341 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    35401 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    35461 cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataacg
    35521 agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag tggttgggac
    35581 gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    35641 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    35701 cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta gcgctttata
    35761 ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag tgggggccag
    35821 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca
    35881 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    35941 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    36001 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt
    36061 tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag
    36121 aaggatttac ttcggagagg attatttcag ataaaaaaaa tccttagctt tcgctaagga
    36181 tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg agaccggtgc
    36241 tctaccaatt gaactacgcc cctaattagg gtggcggaac ggacgggact cgaacccgcg
    36301 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
    36361 tacaaccacc ggttttacga ccggctt
//
LOCUS       DAJTUM010000030        35754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTUM010000030 DAJTUM010000000
VERSION     DAJTUM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..247
                     /locus_tag="OO471_001645"
     CDS             <1..247
                     /locus_tag="OO471_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6242950.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO471_001646"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO471_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6242951.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO471_001647"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO471_001647"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6242952.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3212
                     /gene="mdfA"
                     /locus_tag="OO471_001648"
                     /pseudo
     CDS             1991..3212
                     /gene="mdfA"
                     /locus_tag="OO471_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(3253..3537)
                     /locus_tag="OO471_001649"
     CDS             complement(3253..3537)
                     /locus_tag="OO471_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242953.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3623..4438)
                     /gene="ybjI"
                     /locus_tag="OO471_001650"
     CDS             complement(3623..4438)
                     /gene="ybjI"
                     /locus_tag="OO471_001650"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6242954.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4438..5646)
                     /locus_tag="OO471_001651"
     CDS             complement(4438..5646)
                     /locus_tag="OO471_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6242955.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5730..6266
                     /gene="rcdA"
                     /locus_tag="OO471_001652"
     CDS             5730..6266
                     /gene="rcdA"
                     /locus_tag="OO471_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6242956.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6441..8126)
                     /locus_tag="OO471_001653"
     CDS             complement(6441..8126)
                     /locus_tag="OO471_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6242957.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8396..8773
                     /locus_tag="OO471_001654"
     CDS             8396..8773
                     /locus_tag="OO471_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6242958.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8803..9060)
                     /gene="grxA"
                     /locus_tag="OO471_001655"
     CDS             complement(8803..9060)
                     /gene="grxA"
                     /locus_tag="OO471_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6242959.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9220..9507
                     /locus_tag="OO471_001656"
     CDS             9220..9507
                     /locus_tag="OO471_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6242960.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9491..10213
                     /gene="nfsA"
                     /locus_tag="OO471_001657"
     CDS             9491..10213
                     /gene="nfsA"
                     /locus_tag="OO471_001657"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6242961.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10274..11176
                     /gene="rimK"
                     /locus_tag="OO471_001658"
     CDS             10274..11176
                     /gene="rimK"
                     /locus_tag="OO471_001658"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6242962.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11264..11740
                     /locus_tag="OO471_001659"
     CDS             11264..11740
                     /locus_tag="OO471_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6242963.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12092..13204
                     /gene="potF"
                     /locus_tag="OO471_001660"
     CDS             12092..13204
                     /gene="potF"
                     /locus_tag="OO471_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6242964.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13299..14432
                     /gene="potG"
                     /locus_tag="OO471_001661"
     CDS             13299..14432
                     /gene="potG"
                     /locus_tag="OO471_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6242965.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14442..15395
                     /gene="potH"
                     /locus_tag="OO471_001662"
     CDS             14442..15395
                     /gene="potH"
                     /locus_tag="OO471_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6242966.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15392..16237
                     /gene="potI"
                     /locus_tag="OO471_001663"
     CDS             15392..16237
                     /gene="potI"
                     /locus_tag="OO471_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6242967.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16297..16785
                     /locus_tag="OO471_001664"
     CDS             16297..16785
                     /locus_tag="OO471_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6242968.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16826..17953
                     /gene="rlmC"
                     /locus_tag="OO471_001665"
     CDS             16826..17953
                     /gene="rlmC"
                     /locus_tag="OO471_001665"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6242969.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18128..18859)
                     /gene="artJ"
                     /locus_tag="OO471_001666"
     CDS             complement(18128..18859)
                     /gene="artJ"
                     /locus_tag="OO471_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6242970.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19151..19819)
                     /gene="artM"
                     /locus_tag="OO471_001667"
     CDS             complement(19151..19819)
                     /gene="artM"
                     /locus_tag="OO471_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6242971.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19819..20535)
                     /gene="artQ"
                     /locus_tag="OO471_001668"
     CDS             complement(19819..20535)
                     /gene="artQ"
                     /locus_tag="OO471_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6242972.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20542..21273)
                     /gene="artJ"
                     /locus_tag="OO471_001669"
     CDS             complement(20542..21273)
                     /gene="artJ"
                     /locus_tag="OO471_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6242973.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21291..22019)
                     /gene="artP"
                     /locus_tag="OO471_001670"
     CDS             complement(21291..22019)
                     /gene="artP"
                     /locus_tag="OO471_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6242974.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22237..22752)
                     /locus_tag="OO471_001671"
     CDS             complement(22237..22752)
                     /locus_tag="OO471_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6242975.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23401..25170
                     /locus_tag="OO471_001672"
     CDS             23401..25170
                     /locus_tag="OO471_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6242976.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25381..25704
                     /locus_tag="OO471_001673"
     CDS             25381..25704
                     /locus_tag="OO471_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6242977.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25701..26531
                     /gene="amiD"
                     /locus_tag="OO471_001674"
     CDS             25701..26531
                     /gene="amiD"
                     /locus_tag="OO471_001674"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6242978.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26528..27541)
                     /locus_tag="OO471_001675"
     CDS             complement(26528..27541)
                     /locus_tag="OO471_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6242979.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27640..29070)
                     /locus_tag="OO471_001676"
     CDS             complement(27640..29070)
                     /locus_tag="OO471_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6242980.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29081..30082)
                     /gene="ltaE"
                     /locus_tag="OO471_001677"
     CDS             complement(29081..30082)
                     /gene="ltaE"
                     /locus_tag="OO471_001677"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6242981.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30119..31837)
                     /gene="poxB"
                     /locus_tag="OO471_001678"
     CDS             complement(30119..31837)
                     /gene="poxB"
                     /locus_tag="OO471_001678"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6242982.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31970..32938)
                     /gene="hcr"
                     /locus_tag="OO471_001679"
     CDS             complement(31970..32938)
                     /gene="hcr"
                     /locus_tag="OO471_001679"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6242983.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32950..34602)
                     /gene="hcp"
                     /locus_tag="OO471_001680"
                     /gene_synonym="priS"
     CDS             complement(32950..34602)
                     /gene="hcp"
                     /locus_tag="OO471_001680"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6242984.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34746..35645)
                     /gene="lysO"
                     /locus_tag="OO471_001681"
     CDS             complement(34746..35645)
                     /gene="lysO"
                     /locus_tag="OO471_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6242985.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8910 a         8840 c         9189 g         8815 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtt tatcgtcacc tgtctggcaa
     2281 tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag ggcataagct
     2341 tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa gaggcggttt
     2401 gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta cttggtccgc
     2461 tggtgggcgc ggcgtggatc catgtgctgc cttgggaggg gatgtttgtt ttgtttgccg
     2521 cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc gccatgcgta
     2581 taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg ctgaagaacg
     2641 gccgctttgt ggcgggggcg ctgggattcg ttagcctgcc attgctggcg tggatcgccc
     2701 agtcgccgat tatcatcatt accggggagc agttgagcag ctatgaatat ggcttgctgc
     2761 aagtgcctat tttcggggcg ttaattgcgg gtaacttgct gttagcgcgt ctgacctcgc
     2821 gccgcaccgt acgttcgctg attattatgg gcggctggcc gattatgatt ggtctgttgg
     2881 tcgctgctgc ggcaacggtt atctcatcgc acgcgtattt atggatgacc gccgggttaa
     2941 gtatttatgc tttcggtatt ggtctggcga atgcgggact ggtgcgatta accctgtttg
     3001 ccagcgatat gagtaaaggt acggtttctg cggcgatggg aatgctgcaa atgctgatct
     3061 ttaccgtcgg tattgaaatc agcaaacatg cctggctgaa cgggggcaac ggactgttta
     3121 atctcttcaa ccttgtcaac ggaattttgt ggctgtcgct gatggttatc tttttaaaag
     3181 ataaacagat gggaaattct cacgaagggt aaaaaaatgc ctgattgatt tgtgcgatca
     3241 ggcattctcg aattaatggt gatggtcgtc aatctggtgt tcgataacca tcccttcacc
     3301 tacgctggca agatggcgaa cataaggatg cgggcggtaa gccggagctg gcgcaggagc
     3361 cacatagacg gtttgcggca cagtcgcgac gctgaccgct tgtggaacgc tgactgaatc
     3421 aggaaccaca accactttgt atccactcgg cacatcgacg gtgacacttt gcgccatcgc
     3481 cacaccagta aagcccatta aaagcgcgcc caacagtaga caatttttca taatcatttc
     3541 gcctgaaata atgcagcggg ttaatttccg cgaattatgc agccttcatt caggcattgt
     3601 gttgccgggc gtgctgccgt ttttattggt caaatggcgc ttcgtgttta agaactttat
     3661 cgatcacatc cagtacgcct tcacggttat tgtagcctgc ccggtatttt gccgctgcga
     3721 cgactgcgct gccggcattt tccattgcaa aactaaagcc tgcctgacgc agcatctcaa
     3781 tatcgttacc gccatcgcca aagaccacca cttcgctgtc gtctattccc cataatttct
     3841 gcagttggcg aaggccattg gctttatgta cgccggggat aatcagatcg atgctgccgt
     3901 tgccggtgtg gactggcacc ataatatcgc cgatggcctc atgtaatgct ttttgtactt
     3961 gtggaatcag ttcatcggaa agattcaggc caaacttaaa gaaaatatct tctaagttgt
     4021 caaagttatc gacgtattcc agacggtgat aatacatttc cgccaccgtt ttcatggcat
     4081 cgtcatattt tttgagtgta taggcgctat tttttccgca ggcaataatt tccacttccg
     4141 ggcgcgtcag caaatgttcc acgacagtag caaacgcatc cttcgacagc tcgccattaa
     4201 aaacatcttt gccttcactc attacccagc cgccgttttc agccacaaag gcaatttcat
     4261 tcgcaatttc agggaagaaa gagatcaact gataatattg attcccgctg gcgaccacaa
     4321 agcgaatgcc ttgtgctttc atttgctgat actgagccat aaaccgctca cggttatagg
     4381 ttttttgatc gcttaagaaa gtaccatcca tgtctaccgc aattaattta atgctcatca
     4441 actattctcc atcgccgtct gcgttttggt atcgggtttg gcgacggctt tcgcgacaat
     4501 agccgcgaga ataaccagcg ccagtacaac cagcattgca ctacgtaatc cataatgttc
     4561 gccgagatag cccagcagcg gcggcccgac gaggaaagcc agataaccgg tcgttgctac
     4621 cacactgacg cgggttggtg catcggggcc ggtatcgctg gcggcagaaa tggtcagcgg
     4681 gaagcccagc gaggcaccca gtccccaaag tacaacagac actcctgcga cccaggcgct
     4741 atcgacaaaa ataatcagcc caatacccaa cgcccccatt agtgcactgg cccgaaccac
     4801 ggcaacgcga ctgtaactgt cgatgaacca accgccggtg aagcgtccaa cggtcatccc
     4861 cagggtaaaa ccggcataaa tcagtgagcc ggaagtgggg ctaaaaccgt gaccatcaac
     4921 cattaataag ggtaaccagt cgttggcaga accttcggca aaggccatcg ccagcactac
     4981 aacaccaatc aacagcaact ggatatcgcg ataaaaagga atgccttttt cgccatgctg
     5041 ggtgccatcg gcagcatttt tgcccgtacc gtcagggatt gcctgaatgg cgatataaat
     5101 aggcgcgata cctaccagcg ccgccaataa aatgtgcacc gttgccggaa cgccaaaggc
     5161 cgtcagtgcc atcccgacac cagcgcctgc cagcgtgccc aggctataaa aaccgtgcat
     5221 catcggcaaa accgttttat tcatttctcg ctcaacggcg gcaccttcaa cgtttatcgc
     5281 cacttccgca gaaccaaaac ttgccccaaa gacgccgaga ccaacggcaa agagcagggg
     5341 cgatgtcagc cagagtgcca gacttaatat catcatcccg atcaatgcgc aggacatcgt
     5401 gaccaggatg acattacgtg tcccaaagcg tttcactaac cacgccgagc agagaatacc
     5461 gctcatcgaa ccgatcgaca gaccaaagag aacaccgccc atttcagcga tcgagacaga
     5521 gagaatatcg cggatagcag gcgtacgggt tgcccaggac gccattaaca ggcctggcaa
     5581 aaagaagaac ataaacagcg cccaggttcg gcgtttcaat gcattacgtg aagaatttac
     5641 ggtcatagat cacgtcaaaa taagaagagg gaagacaaca ttagcaaggt tgtgtacatt
     5701 tgtacacaat tgcagacaga ggaaatgaca tgcgtcgcgc taacgatccg caacggcgag
     5761 aaaaaattat ccaggccacg ctggaggcgg tgaaacttta cggaatacat gctgttacgc
     5821 accgcaaaat tgctaccctt gccggggtac cgttgggatc gatgacctac tatttttcag
     5881 gaattgatga gttgttgctg gaggcgttca gcagttttac tgagattatg tcccggcaat
     5941 atcaggcatt ttttagcgat gttagtgatg ctcaaggcgc atgccaggct atcaccgata
     6001 tgatctacag ctcacaggtt gcaacgccgg ataatatgga gctgatgtac cagctctacg
     6061 cgctggctag ccgaaaaccg ctattaaaaa cggtaatgca aaactggatg cagcgcagtc
     6121 agcaaacgct cgaacaatgg tttgaacccg gaaccgcccg cgcgcttgat gcgtttattg
     6181 aggggatgac gctgcatttt gtcaccgacc gtaagccgct atcgcgcgag gagattttga
     6241 ggatggttga gagggttgca gggtagtaga taaattttag ataacaaaaa acccatcaac
     6301 cttgaaccga aatggcgggg ttgatgggct ccacaaaatg gggacatcaa agaaaagcag
     6361 tggcactaat taagactgat gccctgcgga aaagttctgc ggttgtgcaa aaaaatttca
     6421 ttttcagggc aacttcagtt ttatcctaat cctggccata ccatgacgat gattgtccct
     6481 gccagcgtca gcaggacgtt ggcgattgcg taggtgcccg catagcccag cgcagggatg
     6541 ttactgcgag ctgtatcact gatgatctcc attgccggcg cgcaggtgcg tgcgcccatc
     6601 attgcgccga acaacagtgc gcggttcatt cgcaatacat aagcaccgaa caagaaacag
     6661 ataaccacgg gcacaagact gacaattaat ccggcaatca acatctgacc gccaatcgcg
     6721 cccaggccgt tattaatacc gctaccggcg ctcagaccaa cgcctgccat aaacaccatc
     6781 aagccgaact ctttcaccat gcttaatgca ccttgcggaa tgtaaccgaa ggtcgggtgg
     6841 ttagcacgca taaagcccag cataattccg gcgaataaca acccggcagc gttccccatg
     6901 ccgaaactga atgtgctgaa ctggaaggtg atcatcccga tcatcagccc aataacaaag
     6961 aaggcgcaaa atgccagcag gtcagtgacc tggctgtgaa tcgagataaa gccgatgcga
     7021 tcggcgatgg tttttacgcg acgggcatca ccgctgactt gtaaaacgtc acctttgtta
     7081 agcacgacgt tgtcatctat cggcatctca atctggctac gaatgacgcg gttaaggaag
     7141 caaccgtgat cggtcaactt cagttgtgcg agacgtttac ctacagcgtt atggttttta
     7201 acgaccactt cttcagtgac gatacgcatg tcgagaaggt cacgatcgaa aacttcttta
     7261 ccgttacgga agctgggatc gagtcgggca tgggcgtcgg gatagcctac caacgctatt
     7321 tcatcgccca tttgtagcac ggcatcaccg tctggatttg ccagaatccc gttacgtcga
     7381 atacgttcaa tgtagcagcc ggtttgtcga taaataccca gttcacgcag atttttgccg
     7441 tcggtccagg ccaccagctc cgggccgacg cgataggcgc ggatcaccgg taaataaacc
     7501 ttacggttgg catcagtgtc caggccacgt tcgcgggcga tttgctgggc gctggtctgt
     7561 aagtcctgat gctgcaattt cggcaagtaa cgcgcaccaa caatcaaact caccagaccg
     7621 attaaatagg ttaaggcata cccgaggctc agattatcca gtgccagtga gagctgcctg
     7681 ctttccatgc cggaatgatg cagtgtatcg ccagcaccga ccagaaccgg tgtcgacgtc
     7741 atagagcctg ctaacatacc ggccgtcagg ccaatatccc agccaaacag cttacctaac
     7801 cctaaggcga tcaccagcgc actgccaacc atcaccagtg ctaacattag gtaatttttc
     7861 ccatcgcgaa aaaaaatgga aaaaaagttc ggtccggctt cgaccccgac gcagaaaata
     7921 aacagcataa agccaagatt aagcgcatcg gtgttaatgc tgaaatgttg ttggcctaat
     7981 aacagcgata cgactaaaac gccaatggaa ttacccagtt ggatcgaacc aagtcgtaac
     8041 tttccgagac atagcccaag cgcgaggacc acaaataata acagaatgta attcccattt
     8101 aacaattcgg cgacgtttat attcacggag gctaacttct tgtttactag taagctgttg
     8161 aaagaaatgc taatttacga taatgtgttt taccagaatt cagggcgcag attcattcag
     8221 cgcacctaaa cgatagtaaa gtaacaatat attttactag tgtaatcaca ttaggtatca
     8281 acggctatat gaattgcgtt ggcctatatt agcatggaat gcgaagcggc tttatcttac
     8341 tgaacgccac actggcgaaa aatgtgttcg atagacgcag tgtcaggagg aacgagtgaa
     8401 acataaacaa cgttgggcgg gggcaatctg ctgttttgtc ctcttcattg tggtgtgcct
     8461 ttttctggcg acgcacatga aaggcgcttt tcgggctgcc gggcatcctg aaatcggctt
     8521 gctgtttttc attcttcctg gagcagttgc cagtttcttt tcacagcgta gagaagtcct
     8581 gaaacctcta tttggcgcaa tgctggcggc accctgttca atgctcatta tgcggctgtt
     8641 tttttcaccg acgcgctcat tctggcaaga gctggcatgg ttactaagcg cggtgttctg
     8701 gtgtgcgctg ggggcactgt gtttcttatt tatcagtagt ttgtttaaac cacagcacag
     8761 aaaaaatcag taaagccctc aacgcgaggg cttgtcagac gatcaggcgt ccagattttc
     8821 tttcacccat gcagcaaaat cggtatagcc gccgatatgt tgctgatcga caaaaatctg
     8881 cggcacggtt tctacgggtt tacctgcctt ttgttgtaga tcttctttag tgatcccttc
     8941 cgcacgaata tctacatact gatactgaaa atcatcgcgt tcattgctca atttctcagc
     9001 cagatctttt gcacgcacac agtaagggca acccgaacga ccaaaaataa cggtttgcat
     9061 tatttctctc ctcatagatt tatgcctgta atgatcacgc taaaatgtat tcgctgaaag
     9121 taggtttaac ctgttgcatt aattgctaaa agctataact gttaaacaca atacagtgaa
     9181 aagttttaga ctgaaggctc actttgcaga gggaagcgta tgcgcgcgat cggtaaattg
     9241 cctaaaggcg tgttgatact ggaatttatc ggaatgatgc tactggcggt ggcgctgctg
     9301 tcggtaagcg actccctgtc gctgcctgag ccattttctc ggccagaagt gcagattctg
     9361 atgatttttc tcggtgtttt gctcatgctt cccgctgcgg tggtggttat tcttcaggtg
     9421 gcaaaacgtc ttgccccaca gctgatgaac tgtccaccgc aatattcacg ttcagaaaga
     9481 gaaaaagata atgacgccaa ccattgaact tatttgtggc catcgctcca ttcgccattt
     9541 cactgatgaa cccatttccg aagcgcagtg tgaggcgatt attaacagcg cccgtgcgac
     9601 gtccagttcc agttttttgc agtgtagtag cattattcgc attaccgaca aagcattacg
     9661 tgaagaactg gtgacgctga ccggcgggca aaaacacgta gcgcaagcgg cggagttctg
     9721 ggtgttctgt gccgacttta accgccattt acagatctgt ccggatgctc agctcggcct
     9781 ggcggaacaa ctgttgctcg gtgtcgttga tacggcaatg atggcgcaga atgcattaac
     9841 cgcagcggaa tcgctgggat tgggcggggt atatatcggc ggcctgcgca ataatattga
     9901 agcggtgacg aaactgctga aattaccgca gcatgttctg ccgctgtttg ggctgtgcct
     9961 tggctggcct gcggataatc cggatctcaa gccgcgttta ccggcctcca ttttggtgca
    10021 tgaaaacagc tatcaaccgc tggataaaga cgcactggcg cagtatgacg agcaactggc
    10081 ggaatattac ctcacccgtg gcagcaataa tcgctgggat acctggagcg atcatatccg
    10141 ccgaacaatc attaaagaaa gccgcccatt tattctggat tatttgcaca aacagggttg
    10201 ggcgacgcgc taaaaccgcc acgtcgatgt atgatacgcg ggcttttgac caggtctgac
    10261 agagaggtgc agggtgaaaa ttgccatatt gtcccgggat ggaacgctct attcgtgtaa
    10321 gcggctgcgt gaagccgcta tacagcgcgg tcacctggtt gaaattcttg atccgctttc
    10381 ttgctacatg aacataaatc ctgcggcgtc ttctatttac tacaaaggcc gcaagttacc
    10441 ccattttgac gcagtgatcc cgcgtattgg caccgccatt accttttatg ggacggcggc
    10501 actgcgccag ttcgagatgc tggggagcta tccgctcaat gagtcggtcg ccattgcccg
    10561 ggcgcgtgac aaattgcgtt ccatgcaact gctggcgcgt cagggcatcg acctgcctgt
    10621 cacgggcatt gcgcattcgc cggatgatac cagcgattta atcgacatgg tcggtggtgc
    10681 gccgctggtg gtcaagttgg ttgaaggcac gcagggaatt ggcgtcgtgc tggcggagac
    10741 tcgtcaggcg gcggaaagcg tgattgacgc tttccgcggt ctgaacgcgc atattctggt
    10801 gcaggaatat atcaaagagg cgcaagggtg cgatatccgc tgtctggttg ttggtgatga
    10861 agtggtcgct gcgattgaac ggcgggcgaa agagggctat tttcgttcca atttgcatcg
    10921 tggcggcgcg gcaagcgtcg ccagtatcac accacaggag cgtgaaatcg cgataaaagc
    10981 cgcgcgaacg atggcgctgg acgttgctgg tgtggatatt ctgcgtgcta atcgcgggcc
    11041 gttggtgatg gaagtgaatg cgtcgccggg gctggaagga atagaaaaaa ccaccggtat
    11101 cgatatcgcg ggtaaaatga tccgctggat cgaacgccac gctacgacag aatattgcct
    11161 gaaaacgggt ggttagtcgc aatcacatta ctgatcatgg ttttgcctgc gctttttgcg
    11221 taagctgtgc cggtcttttt atcgaaagag gttgtacaaa attatgacat cgctggtcgt
    11281 tcctggtctg gatacgctgc gtcaatggct cgatgacctg gggatgagtt tttttgaatg
    11341 tgataactgt caggctctgc atctgcccca tatgcagaat ttcgacggtg tctttgatgc
    11401 caaaatcgat ctgatcgata acacgatcct gttttctgcc atggcggaag tccgaccttc
    11461 agccgtattg ccgctggcgg cggatttatc tgccatcaat gccagttcgc tgaccgtgaa
    11521 agcatttctt gatatgcagg atgataatct gccaaagctg gtggtttgcc agtctttatc
    11581 cgttatgcag ggcgtaacct atgagcagtt tgcatggttc gtgcgtcaga gcgaagagca
    11641 gatttcgatg gtcattcttg aagctaatgc ccatcaactg ctgttaccga ctgacgatga
    11701 agggcaaaac aacgttaccg aaaactattt tctccactga taattccttt cgagcacgca
    11761 gtcgctgccg cagtgactgc gcgctgcaaa attatctgct gtttttaacc ttttcttaaa
    11821 gattatttca cttctcttgt gtcgatttgg ctttatcaca tagagcaaat acgcataaaa
    11881 atttgttaaa taccgttttt taatccgagc tatagtctca aaccctggct aaagttattc
    11941 ttgcgatgct tctatatagc gagcagtgct ggccgggaga gagttctctt ttcttacacc
    12001 gcgccgataa aaaaatatgc acgtttattg catatctttc agtgtgacaa cttttgttcg
    12061 tttgttaacg aactttcaga aggaaagaga tgtgaccgcc ttaaataaaa aatggctatc
    12121 gggtctggtt gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg ctgaacaaaa
    12181 aacactccac atttataact ggtctgatta tatcgcccct gacacggtgg ccaattttga
    12241 aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag tactggaagg
    12301 caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca gctttctaga
    12361 acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc cggagtggaa
    12421 gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca ataaatttgc
    12481 tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag ttaaagctgt
    12541 gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg aaaatctgga
    12601 aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt ttgctaccgt
    12661 gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca ccggaccggc
    12721 aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat ctcaatacat
    12781 taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg atgtctggca
    12841 ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct cgattccaaa
    12901 agaaggggcg atggcgttct ttgatgtatt cgccatgcca gcggatgcca aaaacaaaga
    12961 cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc atatttccga
    13021 ccatgtgttc tatgtcaacg ccaataaagc agccacgccg ctggtgagtg cggaagtccg
    13081 tgagaacccg ggtatttatc cgcctgcgga tgttcgtgcg aagctgttca ctctgaaagt
    13141 gcaggacccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga agagcggaaa
    13201 ataatccgca gccttagatg ccgggcgggc gcaccacacc cgccggcaat tcgcaccatc
    13261 atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct atccctcgcc
    13321 cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac ctcaccaaat
    13381 cctacgatgg tcaacatgcg gtggatgatg tcagcctgac catctacaaa ggtgaaatct
    13441 tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg ctggcaggtt
    13501 tcgaacaacc ttctgccgga cagataatgc ttgatggcgt cgatttgtca caggttccgc
    13561 cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg catatgaccg
    13621 tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg gaaatttcca
    13681 gtcgggtcaa tgagatgctc ggcctggtgc acatgcagga gttcgccaaa cgtaaaccgc
    13741 atcagctttc tggtggtcag cgacagcgtg tggccctggc ccgaagcctt gccaagcgcc
    13801 cgaaactatt actgctcgac gagccgatgg gcgcgctgga taaaaagctg cgtgaccgga
    13861 tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg atggtcaccc
    13921 acgatcagga agaggcgatg accatggcgg ggcgcatcgc cattatgaat cgtgggaaat
    13981 ttgtccagat tggcgaaccg gaagagatct acgagcatcc gactacccgc tatagcgccg
    14041 aatttattgg ctcggtaaac gtctttgaag gcgtactcaa agagcgtcag gaagatggct
    14101 tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgacgcggat gcctcggtgg
    14161 tcgataacgt gccggtacat gtggcgctgc gcccggaaaa aatcatgctt tgcgaagagc
    14221 cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc tatctcggcg
    14281 atctttcggt gtatcacgtt cgtctgaaaa gtgggcagat gattagtgcc cagctacaaa
    14341 acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt ttgtgctggg
    14401 aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgaataca cttgaacctg
    14461 ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg caaatgaagc
    14521 atgggcgcaa actggtcatt gcgttgccat atatctggtt gatcttgctg tttctgctgc
    14581 catttctgat tgtctttaaa ataagcctgg cggagatggc gcgcgctatt ccaccttata
    14641 ccgaactgat ggagtgggct gacgggcaac tgtccatcac tcttaatctc ggtaattttc
    14701 tgcaactgac cgacgatccg ctctatttcg atgcttatct ccagtcgcta caggtggctg
    14761 cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg gtggcgcaca
    14821 gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg tggacctcgt
    14881 ttctgatccg cgtttatgct tggatgggaa tattaaaaaa caacggtgtg ctgaataatt
    14941 ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tcttcatacc aatctggcag
    15001 tttatattgg cattgtttac gcctacgtgc cgtttatggt gctgccgatt tataccgcgt
    15061 tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca cgaccgctga
    15121 aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc ggatcgatgc
    15181 tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt ggcccggaca
    15241 gcatcatgat cgggcgcgtg ctatggcaag agtttttcaa taaccgcgac tggccggtgg
    15301 cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg tggttccaca
    15361 aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag ttcgttcgcc
    15421 ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa tgctgatgct
    15481 ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tggggcggct ggtcaacgcg
    15541 ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt caagcctgac
    15601 aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg cggtggtgct
    15661 ggtgcgcttt ggcaggtttc gcggatcaaa tggctttgcc tttatgatca ccgcgccgct
    15721 agtaatgcca gatgtcatca cgggcttgtc gctgttgtta ttattcgtcg cgcttgctca
    15781 tgccattggc tggcctgcgg accgcggtat gcttaccatc tggctggcgc atgtcacttt
    15841 ctgtacggct tatgtggcgg tcgttatttc gtcgcgtctg cgggaactgg atcgctcgat
    15901 agaagaagcc gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg tcattacgct
    15961 accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt tgtcgcttga
    16021 tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac cgatgctggt
    16081 cttttccagc gtgcggatgg gggtgaatcc ggaaatcaac gccctggcaa cattgatcct
    16141 cggcgcggtc ggaattgtcg gatttatcgc ctggtatctg atggcgcgcg cagaaaaaca
    16201 gcggatacgc gacatccaac gtgcaagacg tggctgaaga cactaaaatt tgccaacctg
    16261 gctacataat gccgcgcatg tcgcggcatt gttttcatgg aagacgaaac gttgggattt
    16321 tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt gcaggtggcg
    16381 gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa tacgctgggc
    16441 gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt aacgctggtc
    16501 tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc actggtgaaa
    16561 gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc aagttacttg
    16621 tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg tgaatcaaaa
    16681 cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat cctgatgctg
    16741 ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt ataatcgtcg
    16801 cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg gtcgctgtcg
    16861 ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa ccgccgatct
    16921 taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt caggcccgga
    16981 acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa aaacactgct
    17041 cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc tttatccagc
    17101 ctcatttgcg cccgtttttg cggcgctaaa accgtttatc gcccgagcgg ggttaacgcc
    17161 ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg aaagccagag
    17221 tgatggcggc atgatgctgc gctttgtact gcgttctgat accaagctgg cgcaactgcg
    17281 taaggcgctg ccgtggttac acgaacaact accgcagctg aaagttatta ccgtcaatat
    17341 tcagccggta catatggcga ttatggaagg ggagacggag atctacctga ccgaacaaca
    17401 ggcactggcg gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa gtttcttcca
    17461 gactaatccg gcggtcgcca gccagttgta cgccaccgcg cgcgactggg tacgacagct
    17521 gccggttaaa catatgtggg atctgttctg cggtgtgggg ggctttggtt tacactgcgc
    17581 gacgcctgac atgcagttaa ccgggatcga aattgcgcca gaggccattg cctgtgcaaa
    17641 gcagtcagcc gctgaactgg gcttaactcg tttacaattt caggcgctgg actccactca
    17701 gtttgccact gctcaggggg aggtgccgga gctggtgctg gttaacccgc cgcgccgcgg
    17761 cattggtaaa ccgctgtgtg attatctttc aacgatggca ccgcgtttta ttatctactc
    17821 cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt accgcatcga
    17881 acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtgt tgacgctgct
    17941 ggtgaagcaa taaaaaagcc acaggtgcgg cttcagattg ctgatcaggt gcgctttgtt
    18001 catgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgctaa atcaatacat
    18061 tgcagagttg atgtgggccg gataagcgca gcgcatcagg caatcttact tttgtcatca
    18121 gtctcaatta ctgcggaaac cactggtcat tgattttctg atatgtaccg tcagctttaa
    18181 ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga cgtacagcga
    18241 tacccaggcc ggtgccgaag tattgcggat cggtcacttt ttcagtcgca acgcccagtt
    18301 gcggattggt tttcagccat tcgtttacca ccgcggtgtc accaaatacc ccatcaatac
    18361 gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca gttttcactt
    18421 ccgggtgctg atcctgaata tatttctggt gcgtcgtacc gttttccatc ccaatgcgtt
    18481 tgcctttcag gtcggcaaac gttttgtagg tatctttttt ggaaatcacg acggctgagt
    18541 tttcatagta gggtgtggta aacgaaacct gcttgctacg ctccggggtg atatccatac
    18601 cggagattac ggcgtcatat tttctgaatt tcagtgacgg gatcaggctg tcgaacgcgt
    18661 gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc agatcgatat
    18721 caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag gtggctgaaa
    18781 cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca agtaaagcgg
    18841 caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt tttggccgtg
    18901 tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaac atgattaaat
    18961 aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt cattataaga
    19021 atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca ggcaccgtgc
    19081 tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc ggcgaatgta
    19141 cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc agacgcatca
    19201 tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg aacaccatta
    19261 catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc agcgtaatgg
    19321 tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa gaagagagcg
    19381 agcgtttaaa ggcatacggc agcaggatcg ccagcgtatc ttttttgctc attcccaggg
    19441 cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa aacagctgcg
    19501 tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac ggttctgaca
    19561 acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc ccgtaataaa
    19621 tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa ccccgcacca
    19681 gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat gccagaatca
    19741 gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgcagccct ttcatcagct
    19801 cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc gcaggtcaat
    19861 gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg ccgccacaat
    19921 gtaccaggta aatggttcct gggtacgagt agcaatgctt ttggtttgta acattaaatc
    19981 attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc actggttacc
    20041 gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa agatagccga
    20101 tttcgacagc cccagcgcct gaccagattc ccactggccc accggtacag ctttcaacgc
    20161 gccgcgcagc gtttgcgagg cataggcggc atacagcagt gacagagcga tgacaccaca
    20221 aaggaacgga ctcacgtcga agttctcaat gtccatctgc accgggatct gcacgaaccc
    20281 aagattgata gtgaagccat ccgaaagcgt cagcagtaac tgcgaggagc caaaatagat
    20341 aaacagcact accagaattt ctggcaggcc acgcagaatg gttaccagcg ctgaacctgc
    20401 ccacgcgaca ggacgccatt tcgctgactc ccataccgca aagaacatcg ccagcgccag
    20461 cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc ttgctaaagg
    20521 aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg tttcgtaagt
    20581 gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagtt cagtgtttcc
    20641 ctgacgtacc gcgatgccga ggccagtacc gaaataatct ttatcggtca ctttatcgcc
    20701 cactgccgcc agttttgggt tatctttcag ccactcagtg accactgcgg tgtcaccaaa
    20761 tacgctgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc tgtcatacgg
    20821 aacggtggtg atttccgggt gcttatccat aatgaacttc tggtgtgtcg tcccgttctg
    20881 aacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt gctgacccac
    20941 aaacagggca gagttgtcat agtacggggt agtaaacagt acctgctttt cacgctccgg
    21001 agtgatatcc atgcccgcca tcactgcttc tacgcgacgg aatttcaggc ttgggatcag
    21061 gctgtcaaac gcctggttag agaaagtaca ggttgcatca atctctttac acagtgcctg
    21121 cgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt caaacggagg
    21181 atagggggct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa gactaaaacc
    21241 tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc ttagtgagag
    21301 agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc gccttgttct
    21361 acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc cacttcaact
    21421 tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat gatgctgacg
    21481 atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag cagtacctgc
    21541 ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc agaaagatgc
    21601 agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt ttctgcacgg
    21661 gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat caggttttgc
    21721 tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac gttacgacgc
    21781 aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt gcctgcaatg
    21841 ttgagcgtac cggagcgcgg catctcaagc agattgagta cacgcagcag cgagctttta
    21901 cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc cagcgtgata
    21961 tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg aatactcatt
    22021 gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc tttgacagaa
    22081 taattatgca atatttatgc tttaaaagtt aaaagcaaag cgcattattc aataaacata
    22141 gcacaaaata acgggggcgg tggtcggtga gcataaatgt cggcattcct cacgaaatgc
    22201 cggataattt acggggttta ttggttgatc aaggcgttag cgattctcga tggactgacg
    22261 gagcgtaccc gccgtggcat gaacgctacc gcctaagtag cgcacatcgt cgatgaccca
    22321 acactggcct tcctgaatca ttaacacttc atcctgccag ccctggtcac cctgtttgag
    22381 atccacccgc aatggaatgt tacgggcatc acgattaggg atagtcgatg cactggcaac
    22441 gtgggcgctg tctggcaagg tggttcgact ggagaacgga tcgctgctta acaattcacg
    22501 atgactatta tcgcggctgg catcgctaag ttgggttgcc agtttgtcac tcaaataggg
    22561 gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcgtaga attgttgagc
    22621 cacactgtca ggccctccct ggacacagga accggtgcgg gggccgttat ctttataagc
    22681 tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta gagatagtga
    22741 acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta cgaccaaacg
    22801 ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg aacacctgat
    22861 tgggtttgag tccattgctg gcgacccact caaggttgct cgtagctgac ttacttaaat
    22921 tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata tggctgaaat
    22981 gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc tgtagaggtt
    23041 aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga atgcggttat
    23101 tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta agatgattgc
    23161 caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct ttgactctcg
    23221 ataatattat ttatgctaca attgtgggta aagaatttaa gctaatgaat ttgagtgaag
    23281 aataaattaa attgcttcag tatttcgtta attaatataa atacaaaagg cgttgtgctt
    23341 ttataattat aaagcgcgag gagttcggtg gattatcgtt tttcttgggt gatttttaat
    23401 atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact aaatcaaagt
    23461 gataatttcc tggattcact gagggaattt ttcgcgatat taaatcccag tcgtaagggt
    23521 gagttgtcta catgggatac catctacttg catctgatat tagcaataaa tgctgacagt
    23581 gatctcataa aaaatgatgt gttgctcgcg gaaaatattc catcagcgaa ttaccaattt
    23641 aatacatttt ttagcaatac tttcgagatt gatgttaaaa aatatctggg taaatcagaa
    23701 gataacgaag ttgaaataaa agcgggtaat gaaagaatct ctattggaat acgtaatgta
    23761 tcgaatggaa aacttgaaag acaacagttt cttttcccac tggactatga gaataaatta
    23821 caagaacaat tagataagta ttttacgatt gaatcgcacc cattattata caggtatacc
    23881 attggtagta agattgcgaa tgtgattttt gaaaagttat acagtcgtat tgactttaac
    23941 aaagaacaat atatatcctt tataaaggac gcatttattc atttttatga ttatagcagg
    24001 cgatatgcta ttagtgagaa tatcgataaa gacgctgtaa caaacaatat cgcattgatg
    24061 agcacttttt atgacagtga taatacgtca ggtgaggttt taaataatga ttttacagaa
    24121 gaagagtcat ttgaaactgc gttagatgta gagcatgcaa tagtgcttgg atttgctgat
    24181 gataattttg aaacaaaacc tgttcattat caggatttat taacacgatt tagtgccttt
    24241 caagataccg tgtttaatct gtttcctgaa atgcactctt cgcattacca cgatatttgt
    24301 agcgtaagtg tggatatgac aaagggaacg caatgcatga ttcacttgat ggtgaatgaa
    24361 gaagttttca tgtctctgcc tgttccagtt gccacgatgg tacgtgaaga tgcaagtaat
    24421 ttagtcaatc ttaaaacact actcaatgat ggttgtttca ttaaatatag tcattttaat
    24481 gacgtggcat taataaagca aaacataagt aatctctatt tatcacacac tgttgtgaat
    24541 gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt taggtgatgt taaaataaca
    24601 aatagcaatg taataaacag tgcattcaaa aatatttcgt ttagatctgt taaaattaat
    24661 aacgtaaata ctcattcttt aaaatttatt aactgcaatt ttttcaatgt tgatatgata
    24721 agagttaatc tcagcaaatg tctttttcat gaatgtagta tgcatggtgt caagataaaa
    24781 ccctggttgc ctgtaaaatg gacaaaagaa ttaataagtg attatttgta tggctgttta
    24841 ctatctctgt actctatctg tgcaagggat atatataata tgaacgccgg gaataacgtg
    24901 aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaaaaataa atattgtatt
    24961 aaactattaa gcgctcagga tcgggcgttt atatacgagt ttgcaagaat gatcttcgct
    25021 tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc ggctgaccaa
    25081 aaagcaatcc agcggtatag acctcagtca caagatgggg aagactttag gagtcattta
    25141 caatataagt tgcctctttc cgctcattaa tgttgttggt gcaacataat aagacaggta
    25201 agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt aaagacttaa
    25261 gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt cttcccaaca
    25321 tggcatatca gtccgataat gctttagaga tcgaaggctt aacaaacaag gagatcgatc
    25381 atgcaatttt ctacaacccc aactctggaa ggcctgacca tcgttgaata ttgcggtgtg
    25441 gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc cggtatccgc
    25501 gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc acgggagatc
    25561 gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt agttggtatt
    25621 gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag cgtcagcggt
    25681 acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc gctctgttac
    25741 tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag cttgataccc
    25801 gacaccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac tacaccgcag
    25861 atgattttga tagctcgctg gcgacactga ctgataagca ggtcagctcg cattatctga
    25921 tccctgcggt gccaccgcga tacaacggta aaccgcgcat ctggcaactg gtgccggaac
    25981 aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc cttaacgaca
    26041 cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcatccgga gtgaaatatt
    26101 ttgccccgtt tgaacaggca cagattcagg cgcttattcc gctggcgaaa gatattattg
    26161 cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca ccgcagcgca
    26221 aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg attggtgcct
    26281 ggccggatgc gcagcgggtg aacttttacc ttgccgggcg cgcgccgcac acaccggtag
    26341 ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgtaaaa cctgatatga
    26401 caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc ccgacgttat
    26461 ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg gagaaatacg
    26521 ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt ttctcgatgc
    26581 cttcatccag ggtgatgacc ggctgataac ctaactcttc ctgcgcacgc gtaatatcca
    26641 gcgtaaagtc aaaattcaac ttggaggcgc cgtagtgggt cagcggcggc tcttttgctg
    26701 acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg taagggacgg
    26761 aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg ctgcgcagtg
    26821 tgcgatgctc gccgttggtg atgttgtaca cacgcccgga aggtagctta tcgcaggctt
    26881 cctggcttgc cagccacatt gcgtgcacgg cattttcata gtaggtcata tcgaccagcg
    26941 cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc gccagacggg
    27001 gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata gtaaagcgcg
    27061 tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct ttgctgcggg
    27121 caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatattgcga tggtggtgat
    27181 aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc caggcgacag
    27241 cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc tgttgtgtcc
    27301 cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg agcatcactt
    27361 tagcttgtga tgaaaccagc tcggtcagat ccgtcggaac aaactctgcg cccatttttt
    27421 ccagcaattt acccattgct tcgttgcgac cgctcgcacg cacgctgatg cctttctggc
    27481 ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc agtaccttca
    27541 tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt cccccatgat
    27601 gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc tttgttctgc
    27661 cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg ggatcatcaa
    27721 taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga catcgatagt
    27781 acgatagtca cctttatctt ccagggtaaa acacagtcgt cccagccccg gcgctttcat
    27841 gccaaataac aacgtaagtt gctttttcgg ttcaacgaca atcactttcc agctatccac
    27901 cgcatcgccg gtctgtaaat attcgcgctc cgggcggcct ttcgccagat tatgaccgat
    27961 cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat aacgctcttt
    28021 accgccgatt tggttcacca ccagccataa agccgcaagg ctggcggacg ttttaacggt
    28081 aaaccccgcc tgtttggcga aataaccgta ctccggtcgc cagcgggcaa aggcctgagc
    28141 gtcgtagccc cagtcgctgg agttgaccag tttttcttcc tctttcaacg tgctacgtac
    28201 cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg tatcatctgc
    28261 cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg gtacggaagt
    28321 aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg ggatcaacca
    28381 gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac tgagcacctc
    28441 tggtccggcg gcttcgaaga tgcggtgttc gttggctgga tggtctaaca gcgccaccag
    28501 atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc agcgtggcgg
    28561 cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg ctgaacctgc
    28621 gccaacgata attccggccc gaagttcggt cactggtaca ttcgcttcac gaagaatgtc
    28681 cgccgtagcc tgacgagctc gcagatgatc cgactgctca tgtggcgggg cctgcaacga
    28741 actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga cgttgagagc
    28801 cagctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca ccagaaaata
    28861 gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga gatcgacttt
    28921 atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac gtgccgccgc
    28981 taggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa tgtagccact
    29041 ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc aggaatgcgc
    29101 gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc gtcaccaggc
    29161 ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg cctaacgcgg
    29221 cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc atcacatccg
    29281 cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct tcctgcaacc
    29341 gtgcgacgtt attttccagg gcatacatcc cggcggcagc cagaatcccg gactgacgca
    29401 tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca cgattaccga
    29461 cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg aacgaatcac
    29521 aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta aagatgcgcg
    29581 caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa ttcccatgct tctttcaggt
    29641 attcacgcgg cagcactttg ccgttgtggg tgttttccag actgagtaat ttggtgcggg
    29701 cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt agcgtgccgt
    29761 cggcagccgc gtcgatgggt tgcggctgaa tactgcccag caccgctgcg ccaccggctt
    29821 caaacagata gttatgcgcg gcctgaccga caatatactc ttcgccacgt tcgcagtgac
    29881 tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg gcttctttac
    29941 cggaaagctc tgctgcatag tcctgcagag cattaacggt agggtcgtct ccgtaaacgt
    30001 cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt cgggtaacgg
    30061 tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc cacccttttt
    30121 accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg ctgataattg
    30181 cgcgcaacat atacaggcta aaacctttgg cctgttcgag tttgatctgc ggtggaatgg
    30241 ctaattcttc tttggcgacc accacatcca ccaacaccgg accgtcgata gagaaggcgc
    30301 gttgcagggc ttcatcaact tcagaggctt tttctacacg gatacccgta atgccgcacg
    30361 cttcggcaat gcgggcaaag ttggtgtcgt gcagctcagt accgtctgtc aggtaacctc
    30421 cggccttcat ctccatcgcc acaaagccca gcacgctgtt attaaagacg ataattttca
    30481 ccggcagttt catctgcact actgagagga aatcgcccat caacatgcta aaaccgccat
    30541 cgccgcacat ggcgaccacc tgacgttccg gctcggttgc ctgcgcaccc aacgcctgcg
    30601 gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc ttgccgttca
    30661 ttttcagata acgtgccgcc cataccgttg gcgtgccgac gtcacaggta aagatggcgt
    30721 catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg gctttctcgc
    30781 tcggtttagc taaatcgtcc agccctttgc gggcatcgcg gtaatcttcc agcgctttat
    30841 ccagaaactt gcgatcggtt ttttcttcca ccagtggcag caatgcgcgc agggttgatt
    30901 tgatatcgcc gaccagcgcc atatctacct tgctatgcgc gccgatgctg gctggattaa
    30961 tatcaatctg aataattttg gcatcggtcg ggtagaacgc acggtagggg aactgagtac
    31021 cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag aatccgatta
    31081 acccggtcat tccaacatca tacggattat cgtattcgac atgctcttta ccgcgtaggg
    31141 catgaacaat aggtgcttta attttcccgg caaactcaac taactcttta tgcgcccccg
    31201 cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt tgcgccagtt
    31261 tgcgtaactc ttcttcttcc ggcatcacga ctggctgtgg cgcatgatac cagtgcgtgg
    31321 ttgccccttc tggcgcaggt tttaacgcca cgtcgcccgg taatacaaca acggaaacgc
    31381 cacggttaag caccgctttg cgcatagcaa ttgccagcac ttgtgggatc tgctccgggc
    31441 tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg tgggtttcct
    31501 ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc agtaccggaa
    31561 cgttattgcg gtggcaatcg aataggccgt tgatcaagtg caggttgcct gggccgcacg
    31621 atccggcaca gaccgccagt tctcctgaaa gttgtgcttc agcgccagcg gcaaacgccg
    31681 ccacttcttc atggcgggtg gacatccact cgatggtgcc catgcgatta agactgtcgc
    31741 taagaccgtt cagagagtcg cccgtgactc cccagatgcg tttcacgcct gccgattcga
    31801 gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc ctgaatgtga
    31861 taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag aaaggaggca
    31921 ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat tatgcgagaa
    31981 ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg atttcggcgt
    32041 cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt gtcttacagc
    32101 agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt tccagcgcct
    32161 ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt ttggtgaatt
    32221 tcagaccgct ggtcgccgct tctgctactg gggtgaagaa tttctcttta aagaaacgcg
    32281 tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc gggccgcagg
    32341 tcatcacggt acgtgaagct aagtcaggta catctgccag cagttcgcga gtgaggcgac
    32401 cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga tagttacgcc
    32461 actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc cgcacatcgg
    32521 cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg acgccgcagc
    32581 ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat tcccccatcg
    32641 cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac tgggagccga
    32701 caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct ggcgtggagg
    32761 aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt gcatattgcc
    32821 cggcgcgata tgggtagtga tcgtggcaaa tcagggaaat cgtccacaca tccggcgttt
    32881 cttgcgtaat gtgatgaacc tgtatccgcc acgggcattg attcgttggc atcgtcatcg
    32941 acaaactcct tacgcgttca acagttgctt catgtcttct tcaacagtgg tgatagaacg
    33001 caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac caggtgcagt
    33061 cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa tgacaatcgc
    33121 tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttcacac cacagcccag
    33181 tttctctgcc agagtgacag ccagaataat tgctgagtaa gcatcgttac attgacctgc
    33241 atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa agcgatattt
    33301 accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga aatcggtgaa
    33361 gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat gacgcagttt
    33421 ttcacggcta accagatcaa tcagcgtatc ggctgcacca agcagcgtct ggcgaccaaa
    33481 gcccacggtg atcaggtgag gaatttcgct gtacgggaag cccgccattt gttgcgcctg
    33541 ggcgatgacc gcagagaaat cttcgccatc cagatgacgc acgccaggcc agccaacgat
    33601 gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga tgcagttcga
    33661 ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct gccagccgct
    33721 gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat gcgcaggcag
    33781 catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca gcaggttgta
    33841 gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg ctttgacgtt
    33901 gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca gaatgctcat
    33961 cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt tcatatcggc
    34021 aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat cgttgtcgta
    34081 ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc catacaggca
    34141 cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt taggggtaaa
    34201 ttctgctgcc tgacgttgca gctcgccgag atcatcgctc accagttgca ggtcagccat
    34261 cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgtgctttca gcgcctcgcg
    34321 caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt taacgttggt
    34381 cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga tgatgccgta
    34441 ttcacgcgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga gtaaatcctg
    34501 aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc agccgttttc
    34561 tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac cttttaaagt
    34621 tatatttaat atacatgttt aagggtaaga cgcttaacgc ggggataaaa gggatttttc
    34681 atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggaact accgccagag
    34741 aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag aataaaaccg
    34801 tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac gggcagggtg
    34861 aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg gcgaatcagc
    34921 ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa cgccgcgctc
    34981 ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata ccagccgaaa
    35041 ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc gttaattaaa
    35101 ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg gcgattaagg
    35161 acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag gaaaagtagc
    35221 aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag tccactcaga
    35281 ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag cgccatcgca
    35341 atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc tcgctccagc
    35401 cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc agaataatgc
    35461 agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat acccataaaa
    35521 aagagaataa ggtaaaccat ccagcttaat agctgattaa tagcttttaa cgcagcttgt
    35581 tggcgaagcg gaatgaggta acccacaatc aggggaacca gaatgattaa cagcccagaa
    35641 aacatgaaaa tccagtcctt gcaaagaaga agtcgaaatg cgcgatgaca cactactgaa
    35701 agcggaaggg cgagtaaagt tgcaattaaa aggaaatgtt atgcatcaga gggg
//
LOCUS       DAJTUM010000031        35224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTUM010000031 DAJTUM010000000
VERSION     DAJTUM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO471_001682"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO471_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6242986.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO471_001683"
     CDS             1207..1869
                     /locus_tag="OO471_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6242987.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO471_001684"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO471_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6242988.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO471_001685"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO471_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO471_001686"
     CDS             2795..3100
                     /locus_tag="OO471_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6242989.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO471_001687"
     CDS             3103..3507
                     /locus_tag="OO471_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6242990.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO471_001688"
     CDS             3497..3796
                     /locus_tag="OO471_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6242991.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO471_001689"
     CDS             3982..4278
                     /locus_tag="OO471_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6242992.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO471_001690"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO471_001690"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6242993.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO471_001691"
     CDS             5627..5995
                     /locus_tag="OO471_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6242994.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO471_001692"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO471_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6242995.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO471_001693"
     CDS             7203..7904
                     /locus_tag="OO471_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6242996.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO471_001694"
     CDS             7927..8091
                     /locus_tag="OO471_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6242997.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO471_001695"
     CDS             complement(8303..9613)
                     /locus_tag="OO471_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6242998.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO471_001696"
     CDS             complement(9641..10972)
                     /locus_tag="OO471_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6242999.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO471_001697"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO471_001697"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6243000.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO471_001698"
     CDS             complement(12683..13072)
                     /locus_tag="OO471_001698"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6243001.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO471_001699"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO471_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6243002.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO471_001700"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO471_001700"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6243003.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO471_001701"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO471_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6243004.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO471_001702"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO471_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6243005.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO471_001703"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO471_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6243006.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OO471_001704"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OO471_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6243007.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OO471_001705"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OO471_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OO471_001706"
     CDS             21379..21726
                     /locus_tag="OO471_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243008.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OO471_001707"
     CDS             21895..22545
                     /locus_tag="OO471_001707"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243009.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(23166..23542)
                     /gene="rnpB"
                     /locus_tag="OO471_001708"
     ncRNA           complement(23166..23542)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO471_001708"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23575..24720)
                     /gene="garK"
                     /locus_tag="OO471_001709"
     CDS             complement(23575..24720)
                     /gene="garK"
                     /locus_tag="OO471_001709"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6243010.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24817..25707)
                     /gene="garR"
                     /locus_tag="OO471_001710"
     CDS             complement(24817..25707)
                     /gene="garR"
                     /locus_tag="OO471_001710"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6243011.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25737..26507)
                     /gene="garL"
                     /locus_tag="OO471_001711"
                     /pseudo
     CDS             complement(25737..26507)
                     /gene="garL"
                     /locus_tag="OO471_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26523..27857)
                     /gene="garP"
                     /locus_tag="OO471_001712"
     CDS             complement(26523..27857)
                     /gene="garP"
                     /locus_tag="OO471_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6243012.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28232..29803
                     /gene="garD"
                     /locus_tag="OO471_001713"
     CDS             28232..29803
                     /gene="garD"
                     /locus_tag="OO471_001713"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6243013.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29855..30679)
                     /gene="agaR"
                     /locus_tag="OO471_001714"
     CDS             complement(29855..30679)
                     /gene="agaR"
                     /locus_tag="OO471_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6243014.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30928..32208
                     /gene="kbaZ"
                     /locus_tag="OO471_001715"
     CDS             30928..32208
                     /gene="kbaZ"
                     /locus_tag="OO471_001715"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6243015.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32231..32704
                     /gene="agaV"
                     /locus_tag="OO471_001716"
     CDS             32231..32704
                     /gene="agaV"
                     /locus_tag="OO471_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6243016.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32715..33494
                     /locus_tag="OO471_001717"
     CDS             32715..33494
                     /locus_tag="OO471_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6243017.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33484..34293
                     /locus_tag="OO471_001718"
     CDS             33484..34293
                     /locus_tag="OO471_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6243018.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34380..34814
                     /locus_tag="OO471_001719"
     CDS             34380..34814
                     /locus_tag="OO471_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6243019.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34811..>35224
                     /locus_tag="OO471_001720"
     CDS             34811..>35224
                     /locus_tag="OO471_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6243020.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9121 a         9092 c         8414 g         8597 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcgggttttt acgtatatca ggtgaaactg accgataagc cgctttcttt tgggtatagc
    22981 gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt
    23041 tttacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agcgtcgtgg
    23101 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
    23161 aaaacaggtg aaactgaccg ataagccggg ttctgtcgtg gacagtcatt catctaggcc
    23221 agcaatcgct cactggctca agcagcctac ccgggttcag tacgggccgt accttatgaa
    23281 cccctatttg gccttgctcc gggtggagtt taccgtgcca cggactgtta ccagccgcgc
    23341 ggtgcgctct taccgcaccc tttcaccctt acctgatccc gcttgcacgg gccatcggcg
    23401 gtttgctctc tgttgcactg gtcgtgggtt tcccccccag gcgttacctg gcaccctgcc
    23461 ctatggagcc cggactttcc tcccctccac ccgtctcccc cgaagaggac gacgacgaag
    23521 cggcgactgt ctggtcagct tcggcgcgca gtatagaggg tttgcgcgcc cttgtcaccc
    23581 cgtgttgcgc attccaatcg ccagtgtcgc ggcaatatta cgtgaagcac ggcagatatt
    23641 gtcataagcc ccgcggaatg cttcgtccaa cgtacctatg ctggtcaata cgctgaagac
    23701 cgcatcaatg ccatgctgat gtacaacgcc aacatcatcg gtcaggctac ccgcaatgcc
    23761 aatcaccggt ttatggtact tcttcgccac gtttgcgacg ccaatcggta ccttgccgtg
    23821 aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat cgtgaatatg
    23881 ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt ccgcaccaag
    23941 aaacgccatc agcgccgcac ccataccgcc cgcagctcct gcgccgggga catcttttac
    24001 atcaacatgc agcgcttttt taatgacatc ggcatagtga gagaggttat tgtccagctc
    24061 aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg cgccgttatc
    24121 gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt ttaagcgcgg
    24181 atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc caaaaccaat
    24241 ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc ctgcgccgcc
    24301 atcatttgta gcgctaccgc caatgccgat aataatgttt gtcgcgccgc tctccagcgc
    24361 ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc gtttttccgc
    24421 aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt tgccatcgcc
    24481 ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc aggcgtgacg
    24541 ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac cgtcggcaac
    24601 cggaatagaa acgtactgtg catcaggaaa aatttcccga aatccttttt ctatcgcctg
    24661 cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta cgattttcat
    24721 acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc tggattccag
    24781 gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt cgactttcgc
    24841 cagtttttcg tagtagcaca ccagggcgct atgatccgcc gttcctaaac catctgctcg
    24901 cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgcgcgc cgacgccgtg
    24961 ggaagtatcc agcgcatttg ccagatcctt aatatgcaga tcaatacgga agcccggctt
    25021 gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc ccgccagtcc
    25081 accgcgaatt gcctgataca ccaggtccgg gttaacgccc gctttagttg ccagcgttaa
    25141 cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgattcgcca gtttggtgac
    25201 gttacctgca ccaattgccc cggtatgcac cacggaaccc gccattgctt tcatcaaatc
    25261 atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg taccgtcgat
    25321 ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt tcgctttcag
    25381 cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga tcaataccgt
    25441 acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt tcacatgagg
    25501 ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt tagccgtaga
    25561 ggctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac ggtcagcaac
    25621 caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca taatccccag
    25681 gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg tggtggttat
    25741 tttttaaagg tatcagccag tttatgagtg gcagagcgga agacgccgag atcgctgccg
    25801 acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc gaccggcgcg
    25861 aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg aattgctttt
    25921 tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag atcgctgggg
    25981 ccgacgaaga tgccgtctac gccttcggta gcggcaatgg catcgacgtt atctacgccc
    26041 tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc gaaataatcc
    26101 gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat gccttccggt
    26161 gggtaacggg ttgatgccac cgcctgctct gcttcctctt ttgtttctac aaaaggaatc
    26221 aggaagttat agaaaccgat atccagaaga cgcttaataa tttccggctc gttggtcggc
    26281 actcacacta ctggcgcgct ggcgctgcct ttcaaggcca ttaactgcgg aataaacgtg
    26341 gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc agccaaacca
    26401 agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg tacctgtttc
    26461 gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc ttataccttt
    26521 gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta gcagaccatc
    26581 gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg cagttcactt
    26641 accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa gacgttaaag
    26701 acgccgccac agaggccaac aatctctttc ggcgcggtgt cagaaatcac cggccagccc
    26761 agagcaccaa atcctttgcc aaaaaaagcc agcgccatca gcatgaccac cagcgtggtg
    26821 ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag cacaatcggt
    26881 agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga gaagacacct
    26941 cccagcacgc cgcccgcaaa accacacagt gccggaatcg aggcgaccag acccactttc
    27001 agaatcgaca tgcctttttc ctgcaccaga taaatcggga accaggtgag gaagaaccag
    27061 gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg gttagagagc
    27121 aattgcttga tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg ctttttgtgg
    27181 tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttcctc agacatacgt
    27241 gggtgatctg tcgggttatg aatcaacttg atccacagcg ccgtcagcac aaaacaaatc
    27301 acccccataa cggtaaagac gtgctcccat ccccaggcga aagtcagcca gccaagcaga
    27361 ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa agatggcgga ggcagtacca
    27421 cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa ggatggagct
    27481 tccgagaatc ccagcataaa gcgcataaag aacatggaga tccctgccca agccagcggg
    27541 aacatatcaa caaagccttg caggaaggtg aacagcgacc agaaaaagag gctgtaggtg
    27601 taaacttttt tcgagccaaa cttatcaagc aaccagccgc cggggatttg catcagcaag
    27661 taggcccagc caaaagcgga gaagatgtaa cccatcgaaa ccgcacttaa ctgcaactct
    27721 tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt aacggcggta
    27781 acaataaaaa taatcagtaa tattaaatag cgggtatgca cacctttcct tttttcgtca
    27841 acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta ttattatggg
    27901 gaaggtgtta tttataagtt tcatttatgc cgtaacgaca atgaactcgg gaattagtat
    27961 aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac tttttaaata
    28021 ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg ctttaccttg
    28081 ccgggcggca ctgcaatccc taaaatgatt gacattgatc acatttctgc gtttaaactc
    28141 ctgacattct tatttcaccc aatgaaatca tttattttta aatgagacca ggtcctcatt
    28201 ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca gacaagaaac
    28261 gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta ttgttaatga
    28321 taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg aacatattcc
    28381 ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta ttcgttatgg
    28441 cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg acgaatcaat
    28501 ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca aagtcccgga
    28561 acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg atggcagcgt
    28621 gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag gtgtggtgga
    28681 ctatgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt
    28741 ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg cagcagttgt
    28801 acctattcgt accattcaca atatttcgct gaaccctaac tttggcggcg aagtaatggt
    28861 gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa cggatgatgt
    28921 gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg
    28981 ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac aaaaactgaa
    29041 tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc attgcggtgg
    29101 cagcgatgcg ttttctggcg taacggcaaa cccggcggtt ggctatgcgt ctgatctact
    29161 ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg gaagtgcgtg acgcgatcca
    29221 tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga
    29281 gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc cttcgccggg
    29341 caacaagaaa ggcggtctgg caaacgtggt agagaaagcg ctcggctcca ttgctaaatc
    29401 gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt
    29461 aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg tggcttcggg
    29521 tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga tggcggtacc
    29581 cgtcattaaa atggcacccc gcaccgagct ggcgaaccgc tggtttgatt taatggatat
    29641 taatgcgggc accatcgcta ccggcgaaga aactattgaa gaggagggct ggaagttgtt
    29701 ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc aatggggact
    29761 gcataaccag ctggcggtgt ttaacccagc accggtgacc tgataattca attcgtatta
    29821 tgcccgatag cacacgttta tcgggcatat tgagtcacag cgaagacgcg tgatccccga
    29881 ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt
    29941 catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat
    30001 tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac atccgacggc
    30061 ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta
    30121 caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt
    30181 aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg
    30241 cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtact
    30301 tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc acccgatgac
    30361 ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagtg
    30421 cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc
    30481 caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg
    30541 tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc
    30601 gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc ttctcacctg
    30661 aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc
    30721 ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattggc gcaagctact
    30781 ctcgtttcag tgactttcat tacgtttctt ttgtgaatca gatcagaaaa ctattatctt
    30841 tcgttttatt tttatctcac catgacgcag tatcaattga aacaaaacga aagatcaata
    30901 tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac
    30961 aaagcgggca aaacaaatgc aatttatgcc gtttgttccg cacatccgct ggtgctggag
    31021 gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat
    31081 caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt
    31141 tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc tgattctggg tggtgaccat
    31201 ctggggccaa accgctggca aaacctgcca gccgctcagg caatggccaa tgccgatgat
    31261 ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc
    31321 tgtcaggacg atccaattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg
    31381 gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg aagccgatct ggagtatgtc
    31441 attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc
    31501 accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa
    31561 ggtttgaatg ccatctggcc acgcattatt gccctggtgg ttcaacccgg cgtcgaattc
    31621 gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc
    31681 gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatct
    31741 ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc gctgaccttc
    31801 gccctgcgtg aagccctttt ttctctggcg gcgattgaag aagaactggt accagcgaaa
    31861 gcctgttccg gtctgcgtca ggtgctggaa aacgtgatgc tcgaccgccc ggaatactgg
    31921 caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta cagctactca
    31981 gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt
    32041 cgtaatctgg cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcag
    32101 tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tataaaccat
    32161 attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc aaaacaaaga
    32221 ggaacatgct atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca
    32281 ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga
    32341 ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc
    32401 cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca
    32461 gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt
    32521 tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc
    32581 caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg
    32641 ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtggacctct ttaaattact
    32701 ttgagggatt catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct
    32761 ttatcgctgg cctagatatg tttaacggct taacccatat gcaccgcccg gtggtcctcg
    32821 gcccgttggt cgggctggta cttggcgatc tgcataccgg aattttaacc ggcgatacgc
    32881 tggaactagt gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta
    32941 tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg
    33001 tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg
    33061 tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca
    33121 ttgaacgcgt gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt
    33181 tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac
    33241 gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc
    33301 tgctgaaaat catgatgaaa aacgtctaca taccctactt catcctgggc tttgttgccg
    33361 caacctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg atggcgctga
    33421 tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg
    33481 aagatggcat ctaatcaaac caccctgccg aacgtcagcg aaaatgaaga aacactgctg
    33541 actggcgtca atgaaaacgt gtatgaagat cggagcattg ccgcggagct gacgaaaaaa
    33601 gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt
    33661 atgcaggctt ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat
    33721 aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt
    33781 ctggtgacgt ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac
    33841 agtattcaga gcaccaaaat tgccgtcggt gcgccgcttg gcgggattgg cgatgcgatg
    33901 ttctggctaa cgctactgcc gatttgtggc gggataggcg caagcctcgc gctacaaggc
    33961 tctattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtacactt ggggctgcgt
    34021 tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattcccct tattaaagcg
    34081 aataccaaaa aagtcggcca tgctgcgtca atcgttggaa tgacggtaat cggcgcactg
    34141 gtggcaacct atgttcgttt aagcaccacg ctggaaatca cggcgggcga cgcagtagtt
    34201 aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgct ggtctacacc
    34261 ctgaccatgt tctggctggt acgccgcggc tagagtccgc tgcgcctgat tgcggttacc
    34321 gtggttctag ggatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga
    34381 tgttaagtat tattttgaca gggcatggcg ggtttgccag tggcatggaa aaagcaatga
    34441 agcaaatcct cggcgatcaa tcgcagttta ttgccatcga ttttcccgaa acctcaagcg
    34501 ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca
    34561 ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcga
    34621 tgcaaaaacc gggctgtgaa gtgattaccg gcaccaattt gcaactgcta ctggagatgg
    34681 tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac
    34741 accgagggct gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg
    34801 aggaaggaat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggtt
    34861 cgatgaccat cagttgcgtg ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt
    34921 gggcgtgact gaacgcgatg cggaacggct ctgccccgct tacatcgaca cccatgtaca
    34981 cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat
    35041 gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc accgtaaccg cgccgcttaa
    35101 taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc
    35161 gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca
    35221 tccg
//
LOCUS       DAJTUM010000032        34964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTUM010000032 DAJTUM010000000
VERSION     DAJTUM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..783)
                     /locus_tag="OO471_001721"
     CDS             complement(<1..783)
                     /locus_tag="OO471_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6243021.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OO471_001722"
                     /pseudo
     CDS             875..961
                     /locus_tag="OO471_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1366..1842)
                     /locus_tag="OO471_001723"
     CDS             complement(1366..1842)
                     /locus_tag="OO471_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6243022.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO471_001724"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO471_001724"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6243023.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="OO471_001725"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="OO471_001725"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6243024.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..5468
                     /gene="bioF"
                     /locus_tag="OO471_001726"
     CDS             4314..5468
                     /gene="bioF"
                     /locus_tag="OO471_001726"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6243025.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5455..6210
                     /gene="bioC"
                     /locus_tag="OO471_001727"
     CDS             5455..6210
                     /gene="bioC"
                     /locus_tag="OO471_001727"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6243026.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6203..6880
                     /gene="bioD"
                     /locus_tag="OO471_001728"
     CDS             6203..6880
                     /gene="bioD"
                     /locus_tag="OO471_001728"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6243027.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7459..9480
                     /gene="uvrB"
                     /locus_tag="OO471_001729"
     CDS             7459..9480
                     /gene="uvrB"
                     /locus_tag="OO471_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6243028.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO471_001730"
     CDS             complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO471_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6243029.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10845..10989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10977..11966
                     /gene="moaA"
                     /locus_tag="OO471_001731"
     CDS             10977..11966
                     /gene="moaA"
                     /locus_tag="OO471_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6243030.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11988..12500
                     /gene="moaB"
                     /locus_tag="OO471_001732"
     CDS             11988..12500
                     /gene="moaB"
                     /locus_tag="OO471_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6243031.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12503..12988
                     /gene="moaC"
                     /locus_tag="OO471_001733"
     CDS             12503..12988
                     /gene="moaC"
                     /locus_tag="OO471_001733"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6243032.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12981..13226
                     /gene="moaD"
                     /locus_tag="OO471_001734"
     CDS             12981..13226
                     /gene="moaD"
                     /locus_tag="OO471_001734"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6243033.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13228..13680
                     /gene="moaE"
                     /locus_tag="OO471_001735"
     CDS             13228..13680
                     /gene="moaE"
                     /locus_tag="OO471_001735"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6243034.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13817..14521
                     /locus_tag="OO471_001736"
     CDS             13817..14521
                     /locus_tag="OO471_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6243035.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14726..15439
                     /locus_tag="OO471_001737"
     CDS             14726..15439
                     /locus_tag="OO471_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6243036.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15475..16431)
                     /locus_tag="OO471_001738"
     CDS             complement(15475..16431)
                     /locus_tag="OO471_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6243037.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO471_001739"
     CDS             complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO471_001739"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6243038.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17669..18430)
                     /locus_tag="OO471_001740"
     CDS             complement(17669..18430)
                     /locus_tag="OO471_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6243039.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18563..18973
                     /locus_tag="OO471_001741"
     CDS             18563..18973
                     /locus_tag="OO471_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6243040.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18935..20041)
                     /locus_tag="OO471_001742"
     CDS             complement(18935..20041)
                     /locus_tag="OO471_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6243041.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20052..21185)
                     /locus_tag="OO471_001743"
     CDS             complement(20052..21185)
                     /locus_tag="OO471_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6243042.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21178..22914)
                     /locus_tag="OO471_001744"
     CDS             complement(21178..22914)
                     /locus_tag="OO471_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6243043.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO471_001745"
     CDS             complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO471_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6243044.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO471_001746"
     CDS             complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO471_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6243045.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24805..25992
                     /gene="rhlE"
                     /locus_tag="OO471_001747"
                     /pseudo
     CDS             24805..25992
                     /gene="rhlE"
                     /locus_tag="OO471_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO471_001748"
     CDS             complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO471_001748"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6243046.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26801..28951
                     /gene="dinG"
                     /locus_tag="OO471_001749"
     CDS             26801..28951
                     /gene="dinG"
                     /locus_tag="OO471_001749"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6243047.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28979..29941
                     /gene="ybiB"
                     /locus_tag="OO471_001750"
     CDS             28979..29941
                     /gene="ybiB"
                     /locus_tag="OO471_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6243048.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30082..31167
                     /gene="hcxB"
                     /locus_tag="OO471_001751"
     CDS             30082..31167
                     /gene="hcxB"
                     /locus_tag="OO471_001751"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6243049.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31396..31656)
                     /locus_tag="OO471_001752"
     CDS             complement(31396..31656)
                     /locus_tag="OO471_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6243050.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OO471_001753"
     CDS             complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OO471_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6243051.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OO471_001754"
     CDS             complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OO471_001754"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6243052.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32980..34964)
                     /locus_tag="OO471_001755"
                     /pseudo
     CDS             complement(32980..34964)
                     /locus_tag="OO471_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8286 a         8967 c         9442 g         8269 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa
     4741 gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg
     4801 cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg
     4861 atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg
     4921 tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc
     4981 aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg
     5041 ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta
     5101 tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca
     5161 ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc
     5221 gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat
     5281 tgatcgtcgg tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct
     5341 gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa
     5401 cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca
     5461 acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa
     5521 catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa
     5581 tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa
     5641 cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat
     5701 aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg
     5761 acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca
     5821 ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg
     5881 cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct
     5941 aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat
     6001 catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa
     6061 ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag
     6121 ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat
     6181 ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga
     6241 agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg
     6301 gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa
     6361 tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa
     6421 tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc
     6481 gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt
     6541 gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga
     6601 ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat
     6661 taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg
     6721 ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac
     6781 ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa
     6841 tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa
     6901 ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat
     6961 cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg
     7021 ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta
     7081 gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc
     7141 tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca
     7201 ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc
     7261 tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg
     7321 attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc
     7381 agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac
     7441 tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct
     7501 ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac
     7561 cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct
     7621 gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat
     7681 ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac
     7741 tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg
     7801 gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt
     7861 gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat
     7921 ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc
     7981 cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc
     8041 gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg
     8101 gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt
     8161 gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag
     8221 cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg
     8281 ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg
     8341 gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc
     8401 ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg
     8461 ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac
     8521 cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac
     8581 cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg
     8641 acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt
     8701 ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat
     8761 cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg
     8821 accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt
     8881 tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg
     8941 ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa
     9001 gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg
     9061 ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc
     9121 gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa
     9181 cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc
     9241 gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg
     9301 cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag
     9361 aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa
     9421 gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa
     9481 caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag
     9541 gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc
     9601 acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct
     9661 tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg
     9721 cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct
     9781 ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca
     9841 acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc
     9901 aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg
     9961 gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata
    10021 atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc
    10081 gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact
    10141 tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa
    10201 tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta
    10261 aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg
    10321 ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg
    10381 aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc
    10441 gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt
    10501 gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca
    10561 gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc
    10621 aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta
    10681 tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca
    10741 ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatatatg attacatagc
    10801 gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact
    10861 ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga
    10921 ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg
    10981 cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg
    11041 atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca
    11101 ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc
    11161 tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg
    11221 atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg
    11281 gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg
    11341 tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca
    11401 accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca
    11461 ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc
    11521 agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc
    11581 tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct
    11641 ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt
    11701 acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc
    11761 gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta
    11821 acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag
    11881 cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc
    11941 aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa
    12001 gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag
    12061 aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg
    12121 tcgatgaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga
    12181 tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg
    12241 atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag
    12301 tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag
    12361 cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta
    12421 ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc
    12481 atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc
    12541 gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt
    12601 tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga
    12661 cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat
    12721 cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga
    12781 gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat
    12841 ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt
    12901 gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg
    12961 tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg
    13021 tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc
    13081 acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg
    13141 ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt
    13201 tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag
    13261 ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta
    13321 gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc
    13381 ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt
    13441 aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc
    13501 gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg
    13561 cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg
    13621 gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag
    13681 ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg
    13741 tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata
    13801 tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc
    13861 tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac
    13921 cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa
    13981 ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc
    14041 gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct
    14101 gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac
    14161 tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg
    14221 cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc
    14281 gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt
    14341 gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca
    14401 gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt
    14461 gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta
    14521 attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga
    14581 cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat
    14641 ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
    14701 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
    14761 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
    14821 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
    14881 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
    14941 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
    15001 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
    15061 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    15121 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
    15181 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
    15241 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
    15301 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
    15361 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    15421 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    15481 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
    15541 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    15601 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    15661 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    15721 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    15781 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    15841 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    15901 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    15961 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    16021 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
    16081 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    16141 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    16201 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    16261 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    16321 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    16381 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
    16441 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
    16501 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    16561 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
    16621 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    16681 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
    16741 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    16801 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
    16861 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    16921 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    16981 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
    17041 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    17101 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    17161 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    17221 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    17281 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    17341 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
    17401 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    17461 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    17521 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    17581 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    17641 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    17701 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    17761 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    17821 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    17881 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    17941 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    18001 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
    18061 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    18121 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    18181 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    18241 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    18301 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    18361 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    18421 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    18481 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    18541 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    18601 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    18661 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    18721 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    18781 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    18841 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
    18901 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    18961 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    19021 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    19081 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
    19141 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    19201 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    19261 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
    19321 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    19381 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    19441 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    19501 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
    19561 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
    19621 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    19681 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    19741 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
    19801 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
    19861 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    19921 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
    19981 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    20041 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    20101 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    20161 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    20221 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
    20281 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    20341 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    20401 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
    20461 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
    20521 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    20581 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
    20641 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
    20701 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
    20761 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    20821 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    20881 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
    20941 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    21001 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    21061 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    21121 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
    21181 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    21241 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
    21301 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    21361 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    21421 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
    21481 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
    21541 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
    21601 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    21661 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
    21721 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
    21781 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
    21841 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    21901 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    21961 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
    22021 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
    22081 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    22141 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    22201 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    22261 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    22321 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    22381 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    22441 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
    22501 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    22561 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    22621 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    22681 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
    22741 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
    22801 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    22861 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    22921 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
    22981 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    23041 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
    23101 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    23161 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    23221 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
    23281 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    23341 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    23401 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    23461 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    23521 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
    23581 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    23641 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    23701 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    23761 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    23821 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
    23881 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    23941 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    24001 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    24061 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
    24121 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
    24181 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    24241 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
    24301 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
    24361 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    24421 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    24481 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    24541 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    24601 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
    24661 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    24721 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    24781 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    24841 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    24901 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    24961 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    25021 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    25081 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    25141 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    25201 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    25261 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    25321 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    25381 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    25441 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    25501 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    25561 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgt catccgtagt
    25621 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    25681 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    25741 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    25801 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    25861 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    25921 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    25981 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    26041 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    26101 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    26161 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    26221 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    26281 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    26341 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    26401 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    26461 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    26521 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    26581 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    26641 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    26701 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    26761 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    26821 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    26881 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    26941 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    27001 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    27061 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    27121 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    27181 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    27241 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    27301 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    27361 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    27421 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    27481 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    27541 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    27601 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    27661 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    27721 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    27781 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    27841 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    27901 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    27961 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    28021 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    28081 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    28141 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    28201 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    28261 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    28321 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    28381 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    28441 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    28501 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    28561 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    28621 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    28681 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    28741 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    28801 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    28861 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    28921 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    28981 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    29041 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    29101 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    29161 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    29221 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    29281 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    29341 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    29401 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    29461 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    29521 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    29581 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    29641 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    29701 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    29761 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    29821 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    29881 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    29941 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    30001 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    30061 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    30121 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    30181 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    30241 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    30301 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    30361 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    30421 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    30481 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    30541 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    30601 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    30661 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    30721 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    30781 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    30841 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    30901 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    30961 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    31021 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    31081 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    31141 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    31201 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    31261 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    31321 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    31381 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    31441 ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc
    31501 ttccagggca tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt
    31561 ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag
    31621 agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt
    31681 tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa
    31741 atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa
    31801 ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta
    31861 aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag
    31921 ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg
    31981 taaatctttc tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg
    32041 ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat
    32101 ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc
    32161 gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta
    32221 agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc
    32281 gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc
    32341 ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
    32401 ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
    32461 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
    32521 gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
    32581 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
    32641 cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
    32701 aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca
    32761 cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa
    32821 cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
    32881 gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
    32941 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
    33001 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc
    33061 gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac
    33121 ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac
    33181 cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat
    33241 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
    33301 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
    33361 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
    33421 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
    33481 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
    33541 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
    33601 gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
    33661 ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca
    33721 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac
    33781 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
    33841 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
    33901 cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
    33961 gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa
    34021 cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact
    34081 gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
    34141 aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc
    34201 gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg
    34261 ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc
    34321 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
    34381 gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga
    34441 atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg
    34501 cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac
    34561 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac
    34621 attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg
    34681 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
    34741 gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc
    34801 tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
    34861 aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc
    34921 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgc
//
LOCUS       DAJTUM010000033        34902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTUM010000033 DAJTUM010000000
VERSION     DAJTUM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            857..3142
                     /gene="nrdA"
                     /locus_tag="OO471_001756"
     CDS             857..3142
                     /gene="nrdA"
                     /locus_tag="OO471_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6243053.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3376..4506
                     /gene="nrdB"
                     /locus_tag="OO471_001757"
     CDS             3376..4506
                     /gene="nrdB"
                     /locus_tag="OO471_001757"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6243054.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4506..4760
                     /gene="yfaE"
                     /locus_tag="OO471_001758"
     CDS             4506..4760
                     /gene="yfaE"
                     /locus_tag="OO471_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6243055.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4814..5464)
                     /gene="inaA"
                     /locus_tag="OO471_001759"
     CDS             complement(4814..5464)
                     /gene="inaA"
                     /locus_tag="OO471_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6243056.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5643..5885
                     /locus_tag="OO471_001760"
                     /pseudo
     CDS             5643..5885
                     /locus_tag="OO471_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5927..7003)
                     /gene="glpQ"
                     /locus_tag="OO471_001761"
     CDS             complement(5927..7003)
                     /gene="glpQ"
                     /locus_tag="OO471_001761"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6243057.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7008..8366)
                     /gene="glpT"
                     /locus_tag="OO471_001762"
     CDS             complement(7008..8366)
                     /gene="glpT"
                     /locus_tag="OO471_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6243058.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8639..10267
                     /gene="glpA"
                     /locus_tag="OO471_001763"
     CDS             8639..10267
                     /gene="glpA"
                     /locus_tag="OO471_001763"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6243059.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10257..11516
                     /gene="glpB"
                     /locus_tag="OO471_001764"
     CDS             10257..11516
                     /gene="glpB"
                     /locus_tag="OO471_001764"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6243060.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11513..12703
                     /gene="glpC"
                     /locus_tag="OO471_001765"
     CDS             11513..12703
                     /gene="glpC"
                     /locus_tag="OO471_001765"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6243061.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12896..13846
                     /locus_tag="OO471_001766"
     CDS             12896..13846
                     /locus_tag="OO471_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6243062.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13887..14357)
                     /locus_tag="OO471_001767"
     CDS             complement(13887..14357)
                     /locus_tag="OO471_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6243063.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14412..15614)
                     /locus_tag="OO471_001768"
     CDS             complement(14412..15614)
                     /locus_tag="OO471_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6243064.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15701..16243)
                     /locus_tag="OO471_001769"
     CDS             complement(15701..16243)
                     /locus_tag="OO471_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243065.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16522..16947
                     /gene="nudI"
                     /locus_tag="OO471_001770"
     CDS             16522..16947
                     /gene="nudI"
                     /locus_tag="OO471_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6243066.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16986..17588)
                     /locus_tag="OO471_001771"
     CDS             complement(16986..17588)
                     /locus_tag="OO471_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6243067.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17896..19035
                     /gene="arnB"
                     /locus_tag="OO471_001772"
     CDS             17896..19035
                     /gene="arnB"
                     /locus_tag="OO471_001772"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6243068.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19039..20007
                     /gene="arnC"
                     /locus_tag="OO471_001773"
     CDS             19039..20007
                     /gene="arnC"
                     /locus_tag="OO471_001773"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6243069.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20007..21988
                     /gene="arnA"
                     /locus_tag="OO471_001774"
                     /pseudo
     CDS             20007..21988
                     /gene="arnA"
                     /locus_tag="OO471_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21985..22875
                     /locus_tag="OO471_001775"
     CDS             21985..22875
                     /locus_tag="OO471_001775"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6243070.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22875..24527
                     /gene="arnT"
                     /locus_tag="OO471_001776"
     CDS             22875..24527
                     /gene="arnT"
                     /locus_tag="OO471_001776"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6243071.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24524..24859
                     /gene="arnE"
                     /locus_tag="OO471_001777"
     CDS             24524..24859
                     /gene="arnE"
                     /locus_tag="OO471_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6243072.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24859..25245
                     /gene="arnF"
                     /locus_tag="OO471_001778"
     CDS             24859..25245
                     /gene="arnF"
                     /locus_tag="OO471_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6243073.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25239..25511)
                     /gene="pmrD"
                     /locus_tag="OO471_001779"
     CDS             complement(25239..25511)
                     /gene="pmrD"
                     /locus_tag="OO471_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6243074.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25616..26971)
                     /gene="menE"
                     /locus_tag="OO471_001780"
     CDS             complement(25616..26971)
                     /gene="menE"
                     /locus_tag="OO471_001780"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6243075.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26968..27930)
                     /gene="menC"
                     /locus_tag="OO471_001781"
     CDS             complement(26968..27930)
                     /gene="menC"
                     /locus_tag="OO471_001781"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6243076.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27930..28787)
                     /gene="menB"
                     /locus_tag="OO471_001782"
     CDS             complement(27930..28787)
                     /gene="menB"
                     /locus_tag="OO471_001782"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6243077.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28802..29560)
                     /gene="menH"
                     /locus_tag="OO471_001783"
     CDS             complement(28802..29560)
                     /gene="menH"
                     /locus_tag="OO471_001783"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6243078.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29557..31227)
                     /gene="menD"
                     /locus_tag="OO471_001784"
     CDS             complement(29557..31227)
                     /gene="menD"
                     /locus_tag="OO471_001784"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6243079.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31316..32611)
                     /gene="menF"
                     /locus_tag="OO471_001785"
     CDS             complement(31316..32611)
                     /gene="menF"
                     /locus_tag="OO471_001785"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6243080.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32690..32995)
                     /gene="elaB"
                     /locus_tag="OO471_001786"
     CDS             complement(32690..32995)
                     /gene="elaB"
                     /locus_tag="OO471_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6243081.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33050..33511)
                     /locus_tag="OO471_001787"
     CDS             complement(33050..33511)
                     /locus_tag="OO471_001787"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6243082.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33576..34493
                     /gene="rbn"
                     /locus_tag="OO471_001788"
     CDS             33576..34493
                     /gene="rbn"
                     /locus_tag="OO471_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6243083.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34684..34872
                     /locus_tag="OO471_001789"
                     /pseudo
     CDS             34684..34872
                     /locus_tag="OO471_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9338 c         8648 g         8380 t
ORIGIN      
        1 gtaatgcctg aaaggctttg cctgctggcg tgacaactcg ctttgatatc atatccctgg
       61 cataaatcaa tggccgccgc gacaccgtta gcagagaaaa gagatgcaat cattgacggg
      121 agtagagata aatactcctt gcgtagaaag ataatccgca tattaatctg accatcattt
      181 tatataagac ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt
      241 gtccaacccg atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata
      301 attcaagtta ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat
      361 aattttctta taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat
      421 agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact
      481 gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa
      541 gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga
      601 acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc
      661 taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa
      721 gagggtcatt ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt
      781 ctaagcagct tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa
      841 aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga
      901 gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa
      961 cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac
     1021 ctctgacatc cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc
     1081 ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg
     1141 ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata
     1201 cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat
     1261 cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata
     1321 tctagtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct
     1381 tgttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg
     1441 tttttatgac gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt
     1501 gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga
     1561 ttccatcaac gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg
     1621 catcaacgcc ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca
     1681 taccggctgt atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg
     1741 cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag
     1801 cctgctggtg ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta
     1861 cggggtacaa atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct
     1921 gttcagcccg tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt
     1981 tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc
     2041 cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca
     2101 gaacgttgac cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca
     2161 gtctaacctg tgcctagaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga
     2221 gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct
     2281 ggatgaactg gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta
     2341 tcaggattac ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat
     2401 tggtgtgatt aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag
     2461 cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc
     2521 taatgagctg gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa
     2581 agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct
     2641 gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac
     2701 gctttctgcc ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat
     2761 tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt
     2821 ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa
     2881 cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc
     2941 taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct
     3001 gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg
     3061 tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga
     3121 aagcggcgca tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg
     3181 cctacggctc ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc
     3241 cggatgcagc gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg
     3301 cgccagcgtc gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc
     3361 caacaggaca cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa
     3421 gaaccgatgt tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac
     3481 atcttcgaaa agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac
     3541 gtctcccgcg accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc
     3601 agcaacctga aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg
     3661 ctattgccgc ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca
     3721 gaaacgattc attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct
     3781 gttgtgtttg acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc
     3841 agctattacg atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc
     3901 cacaccgtta acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat
     3961 ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt
     4021 tcctttgcct ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt
     4081 gcccgcgatg aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc
     4141 ggcgcggatg atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac
     4201 ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt
     4261 tcgatgattg gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc
     4321 cgcatgcagg cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg
     4381 atcaacacct ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt
     4441 tcttatctgg tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag
     4501 ctctgatggc ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac
     4561 acccttccct tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg
     4621 aaggttactg cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg
     4681 aaccgttagc ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg
     4741 atattgaaat cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc
     4801 tggcctacaa cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag
     4861 ggatcggctc cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac
     4921 gcaagcggcg acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt
     4981 tcacatagat atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct
     5041 taaacgccag tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata
     5101 ctgcatgccg ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca
     5161 gcaacgcgcg ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga
     5221 tgacaccggc ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc
     5281 cgaacggata acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt
     5341 tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt
     5401 caacccagtc gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg
     5461 ccatacccta tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga
     5521 aaatcttaat gaaacgtgtc gtattaatga atttcttttg ccgctacagg tgtgacaccg
     5581 cttttgccct tcatttgcag cgcatagatt ccaccagcca ctaccactaa tagtgcgccc
     5641 agcagataaa atgtgaattt aatcagtcgg atacagcgat gaattttatt cgacagggat
     5701 taggcatcgc cttacaacca gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg
     5761 ttcctcacga accaaccttc tatcgacaga tttcgttgct gactaaagaa aagccggtag
     5821 aaggcagtcc actgttttta ctacaaatgt gcatggaaca attagtggcg attggaaaaa
     5881 tttgatatga gacaggatgg cgcatgaacg ccatcctgca gtaacattac tctttattaa
     5941 gaaattttac tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat
     6001 acagagcatc atataactga ttcacatcag gagtgtattc aggcagttta tctgaccgca
     6061 cggtataagg atgcactacc agtttattct gctgggcatc ttgcaccatg ccagtgagtt
     6121 tgatattacc cggctgcgat gtctcctcaa tcaacatatg gtaatccgga ccaataccat
     6181 ctgcatattc cgccacctgt ttcatggctc ccggcttaaa catccagtcg tagttgtaat
     6241 taacccagct tccatccggc tgtttctgct gcgtttcatt ccagtcggta taggcaatca
     6301 gctgtaccag attgagctcc atgcccattt tgggttccag ctcattctta atacgcttca
     6361 gctcatcagc atcaaaacat tgcaaataaa ctttatcgtc tttaccggtg taaccatatt
     6421 tcttcagcac ttccagcgtt tttgccgcaa tatccttccc ttcctgatga tggaaccacg
     6481 gcgctttgat ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa
     6541 caaattcaat ctcttcttca aaggtgtgca cccggaagtc ggacttaccc attgggaaac
     6601 gccccggata agtctgtact tttttaccgt tttcaatatc gaaaccttcg gtaaatttca
     6661 gcgacttaat ttcatccagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc
     6721 gatccgggaa acgatcggca acatcagtaa cacgatcgag ataatggtca tgcagaacaa
     6781 ccagatggtc gtctttggtc atcaccaaat cctgttccag ataatccgct ccctgcgcat
     6841 acgccatcgc ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg
     6901 cgatgactat tttttcgttg ctgtccgccg ccattgcact gcttcccatg actatagtgc
     6961 tcatcatgat cgccatgcta aggtttttca gcttcaattt catgccatta gcctccgttg
     7021 cgtttttgca gtaattgttc atggcgacgt ttttcgccaa tcatcacaac aatcaacaag
     7081 ataaccgcca gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag
     7141 tccacggtgt agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc
     7201 ccggtaaagc ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga
     7261 ccgatcagca tcacaggacc gtagatcagg aagccgataa caatcataca aatcatatcg
     7321 acggttgggt tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc
     7381 ataaagaaga cgccggttgc cccacggtta ccacggaaga ctttgtccga catccagccg
     7441 cacagcaggg tgcccggaat acccgcatac tcatagaaga agtaggccca ggaggattta
     7501 tccagcgcga aatgcttaac ctctttcaga taagtcggtg accagtcgag gatgccgtaa
     7561 cgcagcagat aaacgaacac gttggcgatg gcgatgtacc acagcagttt gttcggcagc
     7621 acgtactgca tgaagatttg cttcgcggtc agctcctgtt ccgctttttc gttatagtcg
     7681 tccggataat catttttgta ctcttcgatc ggcggcaagc cacaggattg cggggtatcg
     7741 cgcatcatcg caaaggcgaa taatgccacc agaatggcgc agaaagcagg catatagagc
     7801 gccgcatgcc agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca
     7861 ccaccgacgt tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac
     7921 caccagtgca ccatagtacg accacacggc ggccacccca tcccctggaa ccaaccgcag
     7981 aggaacaaca gtacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata
     8041 aacaacatca ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag
     8101 cgatccgata ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa
     8161 agggcaaaac ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcaagc
     8221 gcaaagttct tacgaaccaa atagtaagcc gcatagccaa agaatatccc caggaaaatt
     8281 tgccagcgca atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttgtgt
     8341 ggcgctggtt taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga
     8401 ttaacagcct gattcagtga gagaacctgc cgtttcttga gttgccgcga tgttaagaaa
     8461 acattcataa attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt
     8521 caaaatgacg catgaaatca cgttttactt tcgaatcatg agcgaatatg cgcgaaatca
     8581 aacaattcat gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat
     8641 gaaaactcgc gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc
     8701 cgggattgcc cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga
     8761 catcgcaacc ggtgccaccg ggcgtaacca cggcctgctg cacagcggtg cgcgctatgc
     8821 agtaaccgat gcggaatcgg cccgcgaatg cattagtgaa aaccagatcc tgaaacgcat
     8881 tgcacgtcac tgcgttgaac caaccaacgg cctgtttatc accctgccgg aagatgacct
     8941 ctcattccag gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat
     9001 agacccgcag caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt
     9061 gaaagttccg gatggcaccg ttgatccatt tcgtctgacc gcagcaaaca tgctggatgc
     9121 caaagaacac ggtgccgtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg
     9181 cgcgacggtg tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaactc aggcccttca
     9241 tgcacctgtc gtggttaatg ccgctgggat ctgggggcaa cacattgccg aatatgccga
     9301 tctgcgcatt cgcatgttcc cggcgaaagg atcgctgctg atcatggatc accgcattaa
     9361 ccagcatgtg atcaaccgct gccgtaaacc ttccgacgcc gatattctgg tgcctggcga
     9421 taccatttcg ctgattggta ccacctcttt acgtattgat tacaacgaga ttgacgataa
     9481 tcgagtgacg gcagaagagg ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt
     9541 gatggcgaaa acgcgcattt tgcgggccta ttctggcgtg cgcccgctgg ttgccagcga
     9601 tgacgacccg agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg
     9661 cgatggtctg gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat
     9721 ggctgaatgg gctaccgacg cggtatgccg caaactgggc aacacgcgcc cctgtacgac
     9781 tgccgatctg gcactgcctg gttcacaaga acccgctgaa gttaccttgc gtaaagtcat
     9841 ctccctgcct gccccgctgc gcggttctgc ggtttatcgt catggcgatc gcacgcctgc
     9901 ctggctgagc gaaggccgtc tacaccgtag cctggtatgt gagtgcgaag cggtaactgc
     9961 gggtgaagtg cagtacgcgg tagaaaattt aaacgttaat agcctgctgg atttacgccg
    10021 tcgtacccgt gtggggatgg gcacctgcca gggcgaactc tgcgcctgcc gcgctgccgg
    10081 actgctgcaa cggtttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt
    10141 ccttaacgaa cgctggaaag gcgtgcaacc catcgcctgg ggagacgcac tgcgcgaaag
    10201 cgaatttacc cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc
    10261 gctttgatac tgtcattatg ggcggcggcc tcgccggatt actctgtggc ctgcaactgc
    10321 aaaaacacgg cctgcgctgt gccattgtca cgcgtggtca aagcgcgttg catttctcat
    10381 ccggttcgct ggatttgctg agccatctgc cagatggtca acccgtggca gacattcaca
    10441 gtggactgga atctttgcgt cagcaggcac ctgcacatcc ttactctctt ctcgggccac
    10501 aacgcgtgct cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat
    10561 tgcagggcag cgtagaactg gctcaccagc ggattacgcc gctcggcact ttgcgtgcaa
    10621 cctggttaag ttcgccagaa gttcccgtct ggccgctacc cgcgaagaag atatgtgtag
    10681 tggggataag cgggctgatg gatttccagg cgcatcttgc ggcggcttcg ttgcgcgaac
    10741 tggatctttc ggttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata
    10801 acgccaccga atttcgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc
    10861 cgctgttact tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg
    10921 cctgcttcgg cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt
    10981 cactgatgct tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt
    11041 tacagcgcca gtttgtgcgc caaggtggag tgtggatgcc gggcgatgaa gtgaaaaaag
    11101 tgacctgtaa aaatggcgta gtgaatgaaa tctggacccg caatcacgcc gatattccgc
    11161 tacgtccacg tttcgcggtt ctcgccagcg gcagtttctt tagtggcgga ctagtagcgg
    11221 aacgtaacgg cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg
    11281 gtgaatggta taagggcgat ttttttgcgc cgcaaccgtg gcagcagttc ggtgtaacca
    11341 ctgatgagac gctacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg
    11401 gttcggtgct gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg
    11461 tcagtgcttt acatgccgct caacagattg cccaacgcgc aggaggccaa caatgaacga
    11521 caccagcttc gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg
    11581 ggtgaatccc ggttatccag ggccaaaaca agccgggccg gatggcgagc gtctgcgttt
    11641 gaaagatggc gcactgtatg acgaggcgct gaaatattgc atcaactgca aacgttgtga
    11701 agtcgcctgc ccgtccgatg tgaagattgg cgatattatc cagcgcgcgc gggcgaaata
    11761 tgacaccacg cgcccgtcgc tgcgtaattt tgtgttgagt cataccgacc tgatgggtag
    11821 cgtttccacg ccgttcgcac caatcgtcaa caccgctacc tcgctgaaac cggtgcggca
    11881 gctgcttgat gcggcgttaa aaatcgatca tcgtcgcacg ctaccgaaat actccttcgg
    11941 cacgttccgt cgctggtatc gcagcatagc ggctcagcaa gcacaatata aagaccaggt
    12001 cgctttcttt cacggctgct tcgttaacta caaccatccg cagttaggta aagatttaat
    12061 taaagtgctc aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg
    12121 cgtaccgcta atcgccaacg gctttaccga taaagcacgc aaacaggcaa ttacgaatgt
    12181 agagtcgatc cgcgaagctg tgggagtaaa aggcattccg gtgattgcca cctcctcaac
    12241 ctgtacattt gccctgcgcg acgaataccc ggaagtgctg aatgtcgaca acaaaggctt
    12301 gcgcgatcat atcgaactgg caacccgctg gctgtggcgc aagctggacg aaggcaaaac
    12361 gttaccgctg aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa
    12421 aatgggctgg acgctctaca ccctggagct gttgcgtaac atcccggggc ttgagttaac
    12481 ggtgctggat tcccagtgct gcggtattgc gggtacttac ggtttcaaaa aagagaacta
    12541 ccccacctca caagccatcg gcgcaccact gttccgccag atagaagaaa gcggcgcaga
    12601 tctggtggtc accgactgcg aaacctgtaa atggcagatt gagatgtcca caagtcttcg
    12661 ctgcgaacat ccgattacgc tactggccca ggcgctggct taaactccct tctgatgccc
    12721 ggtaagcatg tggttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt
    12781 gcaacgcagt gaaaattcct ctgaaaacgt ctcgcaaagg ctgaaactgg cagatgtcaa
    12841 aggcctggga taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac
    12901 cgaatcaaca acctcctccc cgcatgatgc ggtatttaaa acctttatat tcacacccga
    12961 aaccgcacgg gattttctcg aaatacattt accagaacca ctgcgcaagc tttgcaacct
    13021 gcaaacctta cgcctggaac ccactagttt tattgaaaaa agtttacgcg cttactactc
    13081 ggatgttttg tggtccgtgg aaaccagcga cggtgacggc tatatctact gcgtgattga
    13141 acatcaaagc tctgcagaaa agaatatggc ttttcggcca atgcgctatg ccactgccgc
    13201 catgcagagt cacctagata aaggctatga cagagttccg ctggtggtgc cattgctgtt
    13261 ttatcatggc gaaacatcgc cctacccgta ctcacttaac tggctggatg agtttgacga
    13321 tccgcaactt gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt
    13381 acctgacgat gagatcatgc aacatcggcg tatagctctg ctggaactga ttcaaaagca
    13441 tattcgcgac cacgatttaa tcggcatggt cgacaggatc accacgcttt tggttagagg
    13501 cttcactaat gacagccagc tacaaacact gtttaattat ctgctgcaat gcggcgatac
    13561 ctcccgtttc acccgtttta ttcaggagat tgccgaacgt tcaccactac aaaaggagag
    13621 attaatgact attgctgaac ggctacggca ggaaggacat caaattggct ggcaagaagg
    13681 taaattagta ggtttgcagc aaggtaaatt agaaggtttg caggaaggta tgcatgaaca
    13741 agccattaaa attgctttgc gcatgctgga acagggcatt gatcgtgacc aagtgctcgc
    13801 ggccacccag ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca
    13861 cagccgatcc ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg
    13921 cctgatttaa acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg
    13981 ccaacagcga caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca
    14041 gccagaaaac cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt
    14101 cgctgcactt ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct
    14161 ggtccaataa acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt
    14221 gccgttttac tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa
    14281 ttctcaatgc gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg
    14341 tagggagggt tggacaaatt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca
    14401 gagtggcgaa atcagacgga taaagtcatg gactcaacaa cctcaatcca gccatgctca
    14461 ctagcgatgt cctggccgtt taaccaacgg cgtagcatat tcagtgccat cattgcgcac
    14521 acttcctgac ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa
    14581 gtgccgtctg gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc
    14641 agtgccagcc cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc
    14701 agggtttctt cctgtgaagg aatcacttca cacgccagca atggagcacc tgcgcgagaa
    14761 agttgcaaag ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg
    14821 ttttgcaatt cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc
    14881 tgtccggcaa cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc
    14941 ggtccggtga gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacgccc
    15001 ggcggcagtt gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta
    15061 gttaaacgca gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg
    15121 atttcgtgct cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg
    15181 caacgattaa gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca
    15241 gcactggcag gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg
    15301 tggaaatagc gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt
    15361 gctgtcgcag cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg
    15421 atcagcacat cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta
    15481 tcccccaccg tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc
    15541 caggcagcgt tagtgtcaac gatttgcccg tataacactt catccccggt ggatagcatt
    15601 tccactttta acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag
    15661 ataaggcgta gtaagaaact gaatgccgcg ccggaaggga tcagaaactg gcgttaaccc
    15721 caacatacag gccgtcagcc acagcgttgt cgcggttacc gtctttaccc gacagattca
    15781 ggtagcgata acccgcctca atactgactg gacgcataat ggtgtaacgc gcgccagcat
    15841 tcgcttcctc ataactttga ataccgctgg agagcgaatc cggagagtag taatactcgc
    15901 caaacaaacg gaagctgttg ccaattttcc actgcaaacc acctcctacc gctgcggcat
    15961 aaccttcatc gccgtaattc gggttggtgt acacgccttt tccgccaacg gtcgccatta
    16021 acggcccgag aggcagattc aaccccagcc ccacgcccgc gacgtcaccg tcgtcgtcgt
    16081 tatgtgtcca gttaccgctt aaagccaggc ccgtcgattc agtcccaaaa ccgacaccaa
    16141 tattggtgta ttctttaccc gcctggccgc tgatgctcat tgcattgacc gatgcagaaa
    16201 ccaacagcat tccggccaga cctgctagcg caattttttt cattatcccc tttcccacaa
    16261 gcaattaatc aaaattccca aagctgcggg attgtactgc ctggcacaga ggattcaatg
    16321 agcgaaaaaa gggatctagt cacttattgt aactaaatac ttcttaaata attaaacgcc
    16381 gaaaaaataa acacacttta ccgatacaca caaaaataat aataagtgca gttatcattg
    16441 caaaagagat tactgtcact ttcacatatt tctgagttac cgtattctca tggtttcttt
    16501 ttatttataa ggaatacccc tgtgcgacaa cggactattg tatgcccttt gattcaaaat
    16561 gatggtgctt atttgctgtg caaaatggcc gacgatcgcg gcgttttccc cggtcaatgg
    16621 gcgctttcgg gtggcggcgt ggagtctggc gaacgaattg aagaggcact acgccgcgaa
    16681 attcgcgaag aactgggaga acagctgctt ttgacagaaa tcacgccgtg gaccttcagc
    16741 gatgatattc gcaccaagac atatgcagat ggtcgcaagg aagagattta tatgatttac
    16801 ctgatttttg actgcgtttc tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac
    16861 tacgcgtggg taaaacctga agatctggtg cattatgatt taaatgttgc cacccgaaaa
    16921 acgttacgtt tgaaaggtct tctttaacaa tgatgacagc catcactggc tgtcagattg
    16981 aaaatttagt ggttaacgaa ttccccatcc agataaactt tgcctttttc cacatgcatg
    17041 actaaaccat ccagataatc aggtttaaat atcgcatcac gcttattttt agcaatatat
    17101 gtcaggcaat gattatgggt gaaaataacg atatttttat caggcgcttt gttttgtaag
    17161 tccttaattg cactataaat ctcattaccg cactgaagaa gtcgtttatc taccgtcagc
    17221 tttttacccg cggaaaacca ggtcgccgac tgaatggtcc ggacggtatt actggaataa
    17281 agatcgaaat cagagatgtc agcgctaaaa gcgttgccca gttcacgggc atcctgggta
    17341 cctttaaccg taatacctgt tttatctgac aagcattgat tggttgaacg atcgcaacgt
    17401 tcagcatggc gaaacaaaac gacaaccggg tgctgctgtg ccagtctggc caatgtttta
    17461 ttgtcgatac gtggcaaacc attgctactc caggcggcat gagtacccag tccggcaatt
    17521 gcagcgagcg ccagtaaaat gatgaaatat ttttttgact tcaatgatga gcggcaaaaa
    17581 gctaacacgt aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc
    17641 gacatttctg gcgataatta gcagttggtg taatattaaa aatcctacga tgtcggcaaa
    17701 atgcctcaaa tttttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata
    17761 tttacgtcta atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc
    17821 caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg
    17881 gcggaaggaa aagcaatgtc agaatttttg cctttttcgc gaccagcaat gggcgtggag
    17941 gaactcgctg cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat
    18001 caggcgcttg agcaagcctt ttgccagttg acgggaaatc agcatgccat cgcggtcagt
    18061 tcagccaccg ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa
    18121 gtgattacgc cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca
    18181 acgccggtaa tggtggatgt cgaccgcgat acgctgatgg tcacgcctga agcgatcgaa
    18241 tcagccatta cgccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca
    18301 gatattgacg ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct
    18361 gcccatgccg tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt
    18421 ttttcatttc atgccattaa aaatattacc tgtgctgaag gtggcctgat tgtaactgat
    18481 aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc acggtctggg tgtcgatgcc
    18541 tatgacagac aaacctgggg ccgtgcaccg caggctgaag tcttaacacc gggctataag
    18601 tacaatctga ccgatattaa cgccgcgatt gccctgacac agttagtcaa attagagcac
    18661 ctcaacaccc gtcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc
    18721 tttcagccat taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt
    18781 cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa
    18841 agaggcattg gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag
    18901 cgttttccca cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg
    18961 ccgctgttcc cggatatgac taccgccgat gccgaccgcg tcatcacagc ccttcagcaa
    19021 ctcgcaggac aataagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt
    19081 cccgtttata acgagcagga aagcttaccg gaattaatca ggcgcaccac cacagcctgt
    19141 gaatcgttgg ggaaagagta tgagatcctg ctgattgatg acggcagtag cgataattcc
    19201 gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc tattttgatt
    19261 aaccgcaatt acgggcaaca ttcagcgatt atggcgggat tcagtcacgt tactggcgac
    19321 ttaattatta cccttgatgc cgatctccag aatccgccag aagaaatccc ccgcctggtg
    19381 gcaaaagccg atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc
    19441 tggtttcgta aaacagcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa
    19501 gcgatgggtg actacggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg
    19561 ttgcactgcc atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt
    19621 gcaattgaaa ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa atacagtttt
    19681 atgcgcctga ttaatttgat gtacgacctg gtgacctgcc ttaccacaac gccgctacgt
    19741 atgctgagtc tgctcggcag cattattgcg attggaggtt ttagcattgc ggtgctgctg
    19801 gtgattttac gcctgacctt cggaccacaa tgggcggcag aaggcgtctt tatgctattt
    19861 gccgtgctgt ttacttttat tggcgctcag tttatcggca tgggattact cggtgaatat
    19921 atcggcagga tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc
    19981 cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg
    20041 gatgcctcgg tattgaagct ctgctggctg ccggttacga aattagcgcc atttttaccc
    20101 ataccgataa tcccggtgaa aaagcctttt atggttcggt ggctcatctg gcggcggaaa
    20161 gagacattcc ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg
    20221 cccaactgtc accagaggtg attttctctt tttattatcg ccatcttatt tgcgacgaga
    20281 tttttcagct cgctcccgca ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc
    20341 gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga aacggaaacg ggcgttacat
    20401 tgcaccgaat ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat
    20461 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
    20521 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
    20581 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
    20641 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
    20701 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
    20761 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
    20821 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
    20881 agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
    20941 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
    21001 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
    21061 gataagccgt tttctgaatc atccacattt tcactttgtc gaaggcgata tcagtattca
    21121 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
    21181 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
    21241 gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac
    21301 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
    21361 cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg
    21421 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc tcccgtttaa
    21481 ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat
    21541 tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa
    21601 acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc acattatcga
    21661 aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc
    21721 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
    21781 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact acggcaaagg
    21841 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
    21901 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
    21961 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatgcc
    22021 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
    22081 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
    22141 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
    22201 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
    22261 atcattcgtg aagcggctaa acatcacgaa gtcggtctgc acgcctggga tcaccatgcc
    22321 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
    22381 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
    22441 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
    22501 tgtcgtgggg ccataccgtt ccgtccactg cttgaatcag gaaaccctgg cactgcgcaa
    22561 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
    22621 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
    22681 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
    22741 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
    22801 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
    22861 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg catcttcgcg tttattgcct
    22921 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
    22981 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
    23041 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
    23101 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
    23161 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
    23221 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
    23281 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
    23341 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
    23401 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
    23461 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
    23521 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
    23581 attttttctg ggttgagcat attcaacgct ttgcactaga tgatgcccaa catagagctc
    23641 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
    23701 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
    23761 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata
    23821 ttctttcttg ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg ctggcagcaa
    23881 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
    23941 gcattattgc cacatttgtg atctccccct ggggaccaat gaacacgccg gtgtggcaaa
    24001 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
    24061 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg
    24121 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
    24181 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
    24241 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
    24301 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
    24361 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
    24421 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
    24481 tcgttcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
    24541 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
    24601 tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
    24661 tcttggtctt gccatgatgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
    24721 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
    24781 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
    24841 cctcgggagt acggtgtaat gtgcctgata tggggattat tcagcgtcat tattgcctca
    24901 gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg
    24961 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
    25021 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
    25081 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
    25141 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
    25201 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct
    25261 gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
    25321 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
    25381 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
    25441 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gtgccagcca
    25501 ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa
    25561 actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt
    25621 gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca
    25681 gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca
    25741 cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc ggccgatgac
    25801 caaactcctt gtcggcaacg gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa
    25861 taacacgctc gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattgtcta
    25921 aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc
    25981 agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct tctgccatac
    26041 tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc ggcagcggca
    26101 aaccaacgtc tgccaggccg tcggcttctt tcgcacatac cgtggaggca aactcggtca
    26161 gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg
    26221 ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg ttaaccagca
    26281 aacgccagag ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atttgctcca
    26341 atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc cacataattc
    26401 cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga tcgccaaacg
    26461 gaatcagcga taacacacct tgcgcactgg caagatgggc ctgataagta tggacagcgg
    26521 ctttcggcaa accggtagaa cccgaggtca acgtcattga gcacagacgc gtcggctgcc
    26581 acgcagcggc atgtacgcct tcaaccagct gaatgtgcag cgacgttaat gccggaaacg
    26641 tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc aattcttcaa
    26701 gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg
    26761 ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct
    26821 caaccacccc ctgcgccgca aagcccgtag ctaactcatc gacgcgagca caaagctcgc
    26881 gccagttgag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc acttgccgcc
    26941 agtgacgcca cggccagtca gagaagatca taacaactgc tcctgtacat cagcgtccac
    27001 taacggcagt gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt
    27061 gtccagtccc ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag
    27121 acctaagctc gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc
    27181 ctgtacctgc tcgcgtactt tttccagact gcccgtgagc gtgggtttga taactaccgc
    27241 gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaggcttt catcccaggc
    27301 aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc
    27361 ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa actgctgacc
    27421 tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc
    27481 cagcaacaga ttcaccacca tgccgtcgcg caccgcttcg tacaatccca ctttgacctt
    27541 cgccactttc tcgcccggca tatcggcaag tttgaggatc agatcgtccg gatcgccatt
    27601 acacagcggt gccgcacggt agttggctgc ttgcggcaac gtatctgtca gttctgccaa
    27661 tgcacagctt acgccaaagg ccacggaagg catctgcggt agctcgcaat cgcctgccag
    27721 ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccagtttt cctggctgaa
    27781 gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta
    27841 cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat
    27901 ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca
    27961 ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt catgtagaac
    28021 agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc
    28081 agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc gcggcaccaa
    28141 cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag gcccatatcc
    28201 agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc ttttttctgc
    28261 ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg
    28321 ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat catgtgcaga
    28381 acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta
    28441 cggatctgac gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag
    28501 ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg
    28561 ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag tgcctggatc
    28621 atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt aatggtgatt
    28681 tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg
    28741 tgccattcaa ccggtgcgta aggcattgct tcatcaggat aaatcatatt gtgtccttta
    28801 gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt tcccgatgcg
    28861 cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagggcgc
    28921 ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt
    28981 tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg
    29041 cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg
    29101 caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct gtgcgaaaac
    29161 gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca ttttgcagcc
    29221 ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg caagccgcca
    29281 tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg atgttgtaac
    29341 taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg ctaatcgccg
    29401 ccgaaccacc atgacctagg agatcaacat acaaccgtga gtagtcggca aacgcctcgc
    29461 ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc agccagggta
    29521 aacctagttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag
    29581 aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt
    29641 ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt tttgcggatg
    29701 atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag
    29761 atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt
    29821 gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt
    29881 gagatcgtaa agcgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg
    29941 ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc ggttgctgta
    30001 caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt
    30061 accaacaaac aattgcccct gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc
    30121 ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg
    30181 gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat
    30241 attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt caatgtcatc
    30301 aacaatccag tactcttctg gttcacagct tgcctgccat tgcaggagcc gtttccccgt
    30361 caggctactt cccagttgca ccacaatttg cgcctgctgc agctcgctgg tcgctttggc
    30421 attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca gcacatcgcc
    30481 aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct cttccgcact
    30541 catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt
    30601 ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcacc
    30661 cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg gttcagcaaa
    30721 cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt cgatggtaga
    30781 aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg
    30841 gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt caattagctc
    30901 cggcgggcga tcggcggtta agagaatcag tttttctccg gttaacccgg cttcaatcag
    30961 tgccggatag agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt
    31021 gctgactttc gccagcccca gcgccagatg ccccaaccca cgctcatcga aatgggtgtg
    31081 gtgaatgaat gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg
    31141 ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca
    31201 gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta
    31261 taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt
    31321 ccatttgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct
    31381 cggggtcgga accacggaca atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa
    31441 tttttgctga gcgcagggaa acgcagaatt cgctttgttg taatgagaga tagcccgcag
    31501 aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt cgcgccagat
    31561 cgcgcggtaa gccagcaact gctgccgttg gctgcaactg atgtaaacag atcacatcat
    31621 ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta cgcagacgca
    31681 gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt tggcagatat
    31741 cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc cactctccta
    31801 actgctgcgc ctgcttatca tcaggatgat ttgctactgt tcccgccagc gcttcagtac
    31861 gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga aaagcatttt
    31921 cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga ctggcagcca
    31981 tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga gcgagcacca
    32041 ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc
    32101 ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca ggcaagggct
    32161 tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc
    32221 tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc
    32281 ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt
    32341 cagcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg
    32401 taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag tagaattgcg
    32461 ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag
    32521 gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta
    32581 gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca
    32641 ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg
    32701 ccagcaacag tcctagtacc agcccgacgg ccgcgcccac accaattcct tgccagggtt
    32761 tttcgtggac gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt
    32821 ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca tgcgctttca
    32881 gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt
    32941 cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta gacattctcg
    33001 ttctccattt gcgtaaaacc tgtcactaac tatagacaag agttgttgat tacgcctgaa
    33061 ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc tccgtcacag
    33121 ggataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag
    33181 gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc tgctgcccca
    33241 ctttttcacc acgcaacgct tcactgacaa tcacccgacc tataacgacc ggctcaagat
    33301 catcatcact tttcagaatc ctcgcatacg ccaccagttc atcatttttc caaccgagaa
    33361 tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg caattctgct
    33421 cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa accgataatt
    33481 cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg tatgccttat
    33541 actagccgga atattttttg aaacgtgatg aactcatgga attaattttt ttaggtactt
    33601 cagccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac ctgcaacatc
    33661 cgacccagag cggactttgg ttgtttgact gcggtgaagg cacccagcat cagctactgc
    33721 ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc
    33781 atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt atccaaccct
    33841 taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg cggattagcg
    33901 gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attctcgatg
    33961 atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt tatggctatc
    34021 gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa gctgctggcg
    34081 tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg ctggaagatg
    34141 gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa gcgctcgcta
    34201 ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca
    34261 tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt cgcggccata
    34321 gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggcaag ctaatcatta
    34381 cccacgtcag ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt
    34441 caattttccc ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt taactctgct
    34501 tattattaaa aacagggtga aacttgccct gttatagcaa cccgcgctac atatatccac
    34561 ccacacaggc atattgagcg atttttgata tttattcatc tccttacgca ctatattcag
    34621 agcaataagt tgcctctttt catcttgatt cagcacaacc tttgcaacta aaaggagccc
    34681 atgatggtta cagttgtcag caattattgt caattatctc aaacccaact cagtcagaca
    34741 tttgcagaaa aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta
    34801 tccggagatg aagaaagtat tgagttactg cataatattg cgttaggtta tgatgaattt
    34861 ggaaaaaaag ctgggtaatg ctgccaactt actgatttag tg
//
LOCUS       DAJTUM010000034        34803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTUM010000034 DAJTUM010000000
VERSION     DAJTUM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..2098
                     /locus_tag="OO471_001790"
     CDS             <1..2098
                     /locus_tag="OO471_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6243084.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO471_001791"
     CDS             2095..3183
                     /locus_tag="OO471_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6243085.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO471_001792"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO471_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6243086.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5053..6465
                     /gene="uxaC"
                     /locus_tag="OO471_001793"
     CDS             5053..6465
                     /gene="uxaC"
                     /locus_tag="OO471_001793"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6243087.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6480..7967
                     /gene="uxaA"
                     /locus_tag="OO471_001794"
     CDS             6480..7967
                     /gene="uxaA"
                     /locus_tag="OO471_001794"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6243088.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8050..8601
                     /locus_tag="OO471_001795"
     CDS             8050..8601
                     /locus_tag="OO471_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6243089.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8606..9850)
                     /gene="sstT"
                     /locus_tag="OO471_001796"
     CDS             complement(8606..9850)
                     /gene="sstT"
                     /locus_tag="OO471_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6243090.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10446..11411)
                     /gene="alx"
                     /locus_tag="OO471_001797"
     CDS             complement(10446..11411)
                     /gene="alx"
                     /locus_tag="OO471_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6243091.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11682..12680)
                     /locus_tag="OO471_001798"
     CDS             complement(11682..12680)
                     /locus_tag="OO471_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6243092.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12759..13451)
                     /locus_tag="OO471_001799"
     CDS             complement(12759..13451)
                     /locus_tag="OO471_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6243093.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13528..14031)
                     /locus_tag="OO471_001800"
     CDS             complement(13528..14031)
                     /locus_tag="OO471_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6243094.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14116..15252
                     /gene="rlmG"
                     /locus_tag="OO471_001801"
     CDS             14116..15252
                     /gene="rlmG"
                     /locus_tag="OO471_001801"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6243095.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15537..15851
                     /gene="higB"
                     /locus_tag="OO471_001802"
     CDS             15537..15851
                     /gene="higB"
                     /locus_tag="OO471_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6243096.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15848..16264
                     /gene="higA"
                     /locus_tag="OO471_001803"
     CDS             15848..16264
                     /gene="higA"
                     /locus_tag="OO471_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6243097.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16309..18327)
                     /gene="fadH"
                     /locus_tag="OO471_001804"
     CDS             complement(16309..18327)
                     /gene="fadH"
                     /locus_tag="OO471_001804"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6243098.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18553..20904)
                     /gene="ygjK"
                     /locus_tag="OO471_001805"
     CDS             complement(18553..20904)
                     /gene="ygjK"
                     /locus_tag="OO471_001805"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6243099.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20921..21340)
                     /locus_tag="OO471_001806"
     CDS             complement(20921..21340)
                     /locus_tag="OO471_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243100.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21318..21671)
                     /locus_tag="OO471_001807"
     CDS             complement(21318..21671)
                     /locus_tag="OO471_001807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243101.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21672..21992)
                     /locus_tag="OO471_001808"
     CDS             complement(21672..21992)
                     /locus_tag="OO471_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243102.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22126..23559)
                     /locus_tag="OO471_001809"
     CDS             complement(22126..23559)
                     /locus_tag="OO471_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6243103.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23622..24071)
                     /locus_tag="OO471_001810"
     CDS             complement(23622..24071)
                     /locus_tag="OO471_001810"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6243104.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24068..27154)
                     /gene="ebgA"
                     /locus_tag="OO471_001811"
     CDS             complement(24068..27154)
                     /gene="ebgA"
                     /locus_tag="OO471_001811"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6243105.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27337..28320)
                     /gene="ebgR"
                     /locus_tag="OO471_001812"
     CDS             complement(27337..28320)
                     /gene="ebgR"
                     /locus_tag="OO471_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6243106.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28538..28869
                     /locus_tag="OO471_001813"
                     /pseudo
     CDS             28538..28869
                     /locus_tag="OO471_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28911..30290)
                     /gene="ygjG"
                     /locus_tag="OO471_001814"
     CDS             complement(28911..30290)
                     /gene="ygjG"
                     /locus_tag="OO471_001814"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6243107.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30708..32228
                     /gene="aer"
                     /locus_tag="OO471_001815"
                     /pseudo
     CDS             30708..32228
                     /gene="aer"
                     /locus_tag="OO471_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32382..33005)
                     /gene="nfeR"
                     /locus_tag="OO471_001816"
                     /gene_synonym="yqjI"
     CDS             complement(32382..33005)
                     /gene="nfeR"
                     /locus_tag="OO471_001816"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6243108.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33282..34027
                     /gene="nfeF"
                     /locus_tag="OO471_001817"
                     /pseudo
     CDS             33282..34027
                     /gene="nfeF"
                     /locus_tag="OO471_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34081..34156)
                     /locus_tag="OO471_001818"
     tRNA            complement(34081..34156)
                     /locus_tag="OO471_001818"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34121..34123),aa:Ile,seq:cat)
     gene            34281..34787
                     /gene="mug"
                     /locus_tag="OO471_001819"
                     /pseudo
     CDS             34281..34787
                     /gene="mug"
                     /locus_tag="OO471_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8487 a         8948 c         9148 g         8220 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgct tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaccgg cacgccccga
     4681 acgttgccat gaaagtttcg tgctaattcg gcttccgtac cggttaaccc gattcatcta
     4741 acaaatgaat gaatcggcaa tttgcattcc gtcgacttat gatttgcgac ggcagaaaga
     4801 taacttgtca tacaacctta aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg
     4861 acattacctg acgacagcga ggccagtact ggcgcggcct gcagcgagat ttaccacttt
     4921 gagagtaatt tttttaacta cgtttattga tctaactcac gaaaatatct tcggactctg
     4981 gaaattggtg tgataacttt gtcagcatcg caccataagc aagctagctc actcgttgag
     5041 aggaagacga aaatgactcc gtttatgact gaagatttcc tgttagatac cgaatttgcc
     5101 cgccgtctgt atcacgacta cgcaaaagac cagccgattt tcgattacca ttgccatttg
     5161 ccgccgcagc agattgcgga agactatcgt tttaaaaacc tgtatgacat ctggttgaag
     5221 ggcgatcact acaaatggcg cgctatgcgt accaacggtg tggctgagcg tctgtgtact
     5281 ggtgatgcgt ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc gcacactatc
     5341 ggcaacccgt tataccactg gacgcacctc gaactgcgtc gtccgttcgg tatcactggc
     5401 aaattgcttt ctccgtcaac tgccgatgaa atctggaacg aatgtaacga actgctggcg
     5461 caggataact tctccgcgcg cggcatcatg cagcagatga acgtgaaaat ggtcggcacc
     5521 accgatgacc cgatcgattc tctggagcat cacgcagaga tcgccaaaga cggctctttc
     5581 accatcaaag tgctgccgag ctggcgtccg gacaaagcct tcaatatcga acaggcgacc
     5641 tttaacgact acatggcgaa gctgggcgaa gtttccgata ccgacattcg ccgctttgct
     5701 gacctgcaaa ctgccttgac caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg
     5761 tctgaccatg cgctggacgt ggtgatgttt gctgaagcga acgaagcgga actggacagc
     5821 attctggcgc gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa
     5881 actgcggtac tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt acagcagtac
     5941 cacattggcg cgctgcgtaa taacaacctg cgtcagttca aactgctggg gccggatgta
     6001 ggctttgact ccatcaacga ccgtccgatg gcggaagagt tgtctaagct gctgagcaag
     6061 cagaacgaag aaaacctgct gccgaaaacc attctctact gcctgaaccc gcgcgataac
     6121 gaagtgctgg gcaccatgat cggtaacttc cagggcgaag gtatgccggg caaaatgcag
     6181 ttcggttccg gctggtggtt taacgatcag aaagacggta tggaacgtca gatgacccaa
     6241 ctggcgcagc tcggtctgct gagccgcttt gtcggtatgc tgactgacag ccgtagcttc
     6301 ctgtcataca cccgtcacga atacttccgc cgcattctgt gccagatgat cggtcgctgg
     6361 gtggaagcgg gcgaagcacc ggcggacatc aacctgctgg gcgagatggt gaaaaatatt
     6421 tgctttaaca atgcgcgtga ctacttcgcc attgaactga actaaggtct ggggttgata
     6481 tgcaatacat caagatccat gcgctggata acgtcgcggt cgctttagca gatttggctg
     6541 aaggcacaga agtcagtgtc gataaccaga ctgttacgct gcgccaggat gttgctcgtg
     6601 gacataaatt tgcgttaacg gatatcgcaa aaggggccaa cgtcatcaaa tatggcctgc
     6661 cgattggtta tgcattggcg gatattgcgg cgggggaaca cgtccacgcc cacaatacgc
     6721 gcacgaatct gagcgatctg gatcagtatc gctatcaact tgattttcag gatctgcctg
     6781 cgcaagcggc agatcctgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc
     6841 gtaacgagct gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc
     6901 agaaccgttt cctgaaagag accaacaacg ccgaaggtac cgacggcgtg ttcctcttca
     6961 gccacaccta tggctgctca caactgggcg acgatcacat caacacccgc accatgctgc
     7021 aaaacatggt gcgccacccg aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa
     7081 acaaccaggt tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt
     7141 tcatgatctg ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc
     7201 tgtataacgt gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg
     7261 gtctggagtg cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc
     7321 gtttctctga ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga
     7381 tgtttggcgc agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac
     7441 tggtcaccat ggtcaacgac ttcaaacagt actttattgc ccatgatcag ccgatctacg
     7501 aaaacccatc accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg
     7561 gctgtaccca gaaagcgggt tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc
     7621 tgaaaacgcc agggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg
     7681 ccctggcggg tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg
     7741 gtggatttgt gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac
     7801 actggatcga ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg
     7861 aagaatttat cgacaccatc gttgagtttg ccaacggtaa gcaaacctgc aacgagcgta
     7921 acgacttccg cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt
     7981 tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgctaa
     8041 ggatttatca tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt
     8101 ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat
     8161 agcgccgtta ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc
     8221 atccttaatg ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg
     8281 atttttattg tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta
     8341 ttgccggtta tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc
     8401 atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg
     8461 tcgataggcg gcacgatgat tgagggtagt tttctgctta tgaatggcct gaatatcatt
     8521 cgtttctggc ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc
     8581 gccatagacg aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt
     8641 cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt
     8701 cgcaagagtc ctgcaatacg ccgataataa agccgacggc aaccacctgc atggcgatat
     8761 cgttcgaaat accgaacata ttacaggcca gtgggatcag cagcaaagaa ccccccgcca
     8821 cgccggatgc gccacaggca cacagagaag ccactacgct caacagcagc gctgtgggca
     8881 gatcgaccgg aatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg
     8941 ctgcgcccgc catattgatg gtggctccca gcggaataga aactgaatag gtatcgcgat
     9001 ccagattcag cttttcacac agcgccatat tcaccggaat gttcgctgca gagctgcggg
     9061 tgaagaaggc atacacaccg ctttcgcgca ggcacagcag caccagcggg aacgggttac
     9121 gacgaatttt ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc
     9181 caaccagcac gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca
     9241 gggtagaaga aaccagccca aaaataccga acggtgcgaa gcgaatgacc agtttcacca
     9301 taaaggtaac ggcattcgac atatcattga ccaggttttt ggtggtctcg ttaccgtgac
     9361 gcagtgcgaa gccgaggccg atcgcccaca ccagaatccc gatgtagtta cctttcagca
     9421 gggcgtcgat ggggttggaa accatgctca ttaccagtcc gcgcatcact tcgacaatgc
     9481 ctgacggcgg cgaaatatca cccgcgctac tggacaagtg cagggtagaa gggaaggcaa
     9541 agctgaagac tactgcggcc agagctgcag agaaggtgcc cagcagatag aggaacaaaa
     9601 taggacggat attggttttc tgcccgtgct ggtggttagc aatagatgcc attaccagca
     9661 tcaacaccag gatgggggca acagctttca gtgcgccgac gaacaaagta cctaacagac
     9721 caacagcttc cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga
     9781 ccaggatttg ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt
     9841 gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga
     9901 cgcatctttc aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt
     9961 cgggaagcga aaaatgctgg attttaaaga gaggtcatgt tttgcaactt aatggttgca
    10021 tttatttaac ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg
    10081 caaaactatt tggaatatga gaacggttgc gctcctttaa cgtgttatgc catataaagt
    10141 cactggcgca gggagcgcgc agggggcggc caatcgccgc cgccccctgc acccccaggc
    10201 tctggcgaac aaaatcgccg ctgcgcggtt ccctcagcct atcccttgcg gctaccgggt
    10261 cgggcgcaag gtaacgtccc tgtaagatgc gccctcgccc cggcctccag ggaacgcttc
    10321 ggcgattttg acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta
    10381 cataattaat agctcacctt tgtctggaat gagttatctc taggaatggc ttaatataca
    10441 gcgaatcatt ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac
    10501 gtcatcacca gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag
    10561 tcgacaatca gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc
    10621 atcgagaaac gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatt
    10681 gcaaacaggt ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga
    10741 atgctatcca cggcaaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc
    10801 ggtgtggcgt acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg
    10861 gtcatgcgca aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca
    10921 tcttcatggg cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac
    10981 aggatccagt cgaactgcga aatcagccag ctgcctgtga agatcatgat ggtacgcaga
    11041 acaatcgccc cgagcacgcc atacaccagc acgcgacgtt gtaatgccgc cggaacggag
    11101 aaatagctga acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc
    11161 agataacccg tgagaaaggc cagggcctgt ggatcggcaa cggcgcgacc ttcggtttgc
    11221 accagatacc accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac
    11281 caggccgcag cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg
    11341 tcgatagcca gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg
    11401 acagtattca tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc
    11461 gctgcttttt atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa
    11521 tacgtctgcg tattcctttc gggttgcccg gtgaccggat gtggtttttc actcatcgta
    11581 atgacgatcg accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca
    11641 ttcagtcagt caatggtctg ttacatacct tttacactgt tttactttgg caagtatagt
    11701 tttacgctat ctgccggaaa aatcacccca gtttggcggc ggatctcggt cagcagtttg
    11761 gctgtgatgc gactgaccgc cagccctgga tgatccacca gatgctcatc caccagggta
    11821 gcgaacagct ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc
    11881 atctggctgc cacgcggcac aaagcagact ttctggcatt cagacagttt ttcaatcacc
    11941 agcgatcccg cttcgccctg aatctcgctt cccaggacag aatcactgac ctttgagtgc
    12001 tgcaaggtga cgctgaaatc accgtaatcc atcaccacca cgccgtgggc gtcaacgccg
    12061 cttgccagca aactcgccgt tgcctgcacg cttttcggct caccaaataa cgccaccgcc
    12121 gacgccaggc agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag
    12181 gtgttgggat tctcaccgtc caggtaacgt tgataccgcg aggaatactg gcaatagttg
    12241 aaaaagactt tacgcagttt gcctgctttc ggcagcgcct ggcgcaacaa atgaaagttc
    12301 ggcaggcagg cggttttaaa tgcctcaaac agcaccacct gattttcgcg cgcacaggca
    12361 atggcggcat ccacttccgc cagattcgac gccagcggtt tttcgcaaat cacatgaatt
    12421 ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg aattcgggct ggcaatatac
    12481 accgcgtcaa tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca
    12541 gaaaaatcat tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac ggcggttaac
    12601 ttgtatttac cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca
    12661 atcacagcga aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt
    12721 agcacgcctt tttcatcttc gacgtgcgca gatacgcatt attctttcgc cggacagcca
    12781 tgctcactaa acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg
    12841 atttttgcgt caatcaccgc gctgacacga gcgagtttct cccgcagttg agtataaaaa
    12901 ccgtgcttca tattgaggtc tggcgcgtta aacccgatag cgtcgatgcc gtactgtttt
    12961 gccagccaga tggtgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttcc
    13021 ccaaacacct ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcaa
    13081 aagatcactt tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgatgcttca
    13141 tcgtaatttt tacgtccgtt atcgccactc accagtagcc atttcacttt tccggcgtgg
    13201 tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc
    13261 agcaataaac ccacgttacg cgccgggacg gcgttgacat cgccccaggt cagctgcttg
    13321 ctggcgttga ccataacgcg atcggcgata aaaatggtcg ctcctgccac aagtaacagt
    13381 aagcaggcaa tctttagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcaaat
    13441 gcgcgcagca tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga
    13501 aagaaaagat aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt
    13561 tagccacagc cgggtgtcga actcaagctg gtggtactgc ggctccatat ggcagcacaa
    13621 ttgatagaac gctttgttat gctctttctc cttcaggtgc gccagctcgt gcaccacgat
    13681 catgcgcaaa aacggttccg gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt
    13741 tgctttcagc ttgctgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt
    13801 tagcacatgg atcttattgt catacatcac tttattgatc ggcggggcat tacgcagaaa
    13861 ctgatttttc agatcctgag tatattgcca gagagcttta tcggtggcgt agtcgtgtgt
    13921 tcccggatag cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac
    13981 ctgcgaaagc agctgctcgg gataaccctg aagataagta agattgctca taaccgccca
    14041 cgaaaaagag aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat
    14101 cattcaggag ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt
    14161 tttccggcga cggatgacgt taacccgcta caggcgtggg aagcggcaga tgaatatttg
    14221 ctgcaacagt tggacgacac agaaatcctc ggcccggtgt tgatcctgaa tgatgccttt
    14281 ggtgcgttaa gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc
    14341 agtgaactgg cgacgcgcga gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag
    14401 tttctcgaca gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg
    14461 aaaacactgg cattgctgga acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat
    14521 acacgtatta ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc
    14581 gaaaaagtgc tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat
    14641 tgcacgttca atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa
    14701 ggtactgact ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc
    14761 ggcgcgcgct tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc
    14821 ggttgcggta atggcgttat tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg
    14881 gtgtttgtcg atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac
    14941 atgccagagg cgttggatcg cagcgagttt atgatcaaca acgcgctctc cggcgtggag
    15001 cctttccgct ttaatgctgt gctctgcaac cctccgtttc accaacaaca tgcgctgacc
    15061 gataacgttg cctgggagat gttccactac gcccgccgct gcctgaaaat caacggcgag
    15121 ctgtatatcg ttgccaaccg ccatctggat tacttccata aactgaagaa aattttcggc
    15181 aactgcacca ctattgcgac gaataataaa tttgtggtgc tgaaagcggt gaagctggga
    15241 cgtcgtcggt aagattggga gttacctgcc ggatgcggcg taaacgcctt atccggccta
    15301 cgttcagcac gttaccccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc
    15361 tgctcagttc tggaatcgcg cttccataaa acaaaaaatc cctgtcacca tcctctacat
    15421 tctcttgttt agcgtttttc tacgtttatt cttccgtcac acagataaat tcatccgttg
    15481 ccaatctgtc aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc
    15541 acctgataac tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg
    15601 agttggtggc tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa
    15661 aagcagtatt cccatctctg gataacttca aatatctgga taagcattat gttttcaatg
    15721 ttgggggcaa tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca
    15781 tacgtgaagt tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg
    15841 ggaaaaaatg attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc
    15901 accttttctg gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga
    15961 tcatctgctg ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac
    16021 cgcgtgggaa gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc
    16081 tggcggtata gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct
    16141 gccggaaatt ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac
    16201 gctggaacac gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga
    16261 ttaacgcgac gtgccgaatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag
    16321 cgccagccgt gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca
    16381 gccgccaatt aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt
    16441 tggctcttgc cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt
    16501 gatcaccaca tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt
    16561 cacaccccgc gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg
    16621 tcccggtttg ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg
    16681 cattccctgc gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt
    16741 acagaacctg gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt
    16801 atcaaaacca atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc
    16861 gcgcagtaca tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac
    16921 acgcggcacg atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt
    16981 cacggtgtga ttgagtttca gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg
    17041 cagcgtttcg taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc
    17101 gatctcgctg tgcgcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc
    17161 aaacgccagc ccagcaggtc cagcgccgac caccgccaga ttttttttct gcacggcggg
    17221 aaggattggc attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt
    17281 gccaacgaag atctgatcga ggcagccctg gttgcagcca atacaagtgt tgatctcatc
    17341 ggctcgccct gattgcgctt ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat
    17401 cgatactata tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat
    17461 ccggttggtg gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct
    17521 aaatgcgccg cgcggcacgg gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat
    17581 tccggtgttg ataatggtcg cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc
    17641 ggcaaaagtg ccgccgcctt cgactaggtc gagcatcgac aaccggtaaa taataatgaa
    17701 gtcgttgccg acgcgttcgc gcaccgtacg cactacttct acggcaaatc gcatccggtt
    17761 gcggtaatcg ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt
    17821 gatcaaatac ccttcagagc ccatcacttc gacgccatcg tatccggcct cccgcgccag
    17881 ttgcgcgcag cgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg
    17941 aggaacgaaa cggttgatgg gggcctgcaa tgcggacggt gcgaccagat gcggttggta
    18001 gctgtagcgc ccggtatgca aaatttgcag ggcgattttg ccgccttcct gatgtacggc
    18061 ttcggtaatg gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc
    18121 catgccaacg cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg
    18181 acgggcgcgt tcggcataaa acgcagccag ccgttcagca ccgtccgggt attcctccag
    18241 cccggtgtgc attgaaccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag
    18301 cggggcgaac agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt
    18361 agtcggatga gttctaattt agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt
    18421 tgtgatggga ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt
    18481 tttatccggc ttgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc
    18541 agccgttgca aattactgct tacggaaaaa atcgttatac aacatataca aatgagcggc
    18601 actccaggag aaatttggtg cgccttgctg cgcgcctgtc agcgggttgt aattctcctg
    18661 aattgggcca tcggcggtta atcctttggc gtgcctgaag aacgtatccg ccagcttcag
    18721 ggcatcatca cgataaccgt aacgctccat ccctttcaga ccaaaccaga actgatccac
    18781 ccatacgcgc ccgcgccagt agatatcagc gccaaaagcc ggattggtta acgccgccgt
    18841 tcccagcgga acaaatgtgt tgaactcttt aggatcgagc atcaccttca ccaccgcgtc
    18901 ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg gccctttacc
    18961 gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt caatacgtac
    19021 gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg
    19081 ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg ttgccatctc
    19141 cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact cctgcaatag
    19201 cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca ccgtccagtc
    19261 gctacgtttg ccgccattag cgacatattt atccaactgt tctttgtcga taaaaccaaa
    19321 gacggcggca tcatcacgcc ccgattccca cgacgcagcg acctgtgccg gaatttccag
    19381 actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc
    19441 ttctttatcg ccttttttca ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt
    19501 gtcgcgggtc gcgccatatt ccggcacccc gttgccgtta tgatcgcggt tacgtaacca
    19561 ccagtcgtga taggccacca gtttcgggta catctctgcc aaccaggctt tatcctgggt
    19621 gacgttgtat acttccatca ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt
    19681 ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca ggtcggggac
    19741 aaagcccaca tcctgcggac gcacgctgtc gccaggctgg atctgccagg agaagaccgc
    19801 gcggatattc tctttggcga tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca
    19861 ggtatcccac ggccaggtct gattgccgga gaaccagcgt ccggtcaccg acggcgtgac
    19921 ggtgttataa ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc
    19981 tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc ctttcttcag
    20041 gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg ccagaatatc
    20101 gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg aataggtggt
    20161 gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac cgttgatttc
    20221 agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg tcagcagatc
    20281 ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg cgctgatttt
    20341 gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt tcccttcttt
    20401 cgcttccagt ttttccagca gttcgccatc ccacaccaga tccagcggtt tattgctgat
    20461 gattttagtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg
    20521 cacatctttt gctgtcagtt tttgcaccag cgcgccggga atactgtatg cctccagcgt
    20581 gaagttgact ttcttgccgt cttgccagac agtcaggcgg tcgaaattgc tggccataaa
    20641 gttgatgtat tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc
    20701 gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa aacgctggtg
    20761 atcgtcgtaa tcgtaatcct tcatgtactg cggcgcacca gtacggttaa tcacgttttt
    20821 ataattgtcg gcgttagcgg catgagcgga aaaacttatc agcagagcgc aggttactgg
    20881 cgttaaaata gttttgattt tcatgagatt acagtcctta ttaccagtag aaatattgca
    20941 tcaggaagat ttggtcgctg ggatcgttag aggcattttt catgtagaag cggctatcaa
    21001 aagccgtggc gaaacgtccg ccgccatatt cgtaccagat accaaactgc atgaaagagg
    21061 tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg gtggagagat
    21121 aaacgccttt taacgcttca tacatggcgc tcaccatcat cccgctttca cttcccggta
    21181 cgtccagccc gtcggcacgt ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa
    21241 acaggcccgt gagatagagt tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg
    21301 tgatgccttc ggcaaggtca tactggatgt aaccgttcat ccattgccgg ataggtgtat
    21361 ttgtcgtcgt agcgatcgta cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt
    21421 ccggcaggtg tggcggtgac gctataacga aagttgccgc ttaagttctg taccttcagc
    21481 gcggtcataa tgtcgcgggt attgggaatg acgtagccca aatcgggtgt gaagtcgccc
    21541 caccattgca ggtcatccag cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca
    21601 tcattggtac gataaccgcc atagaaacgg ttaatcccac cttcgaaacc accccagcta
    21661 tggtcaggca cctaggcatt accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa
    21721 atcaggccga accaggagcc gtacttcatc tccaggccac cttcaatata ggtgccgtcg
    21781 ctgtatttat gtttattctc accctctaaa tccatatatc caccaagccc taattcgccg
    21841 taaaaacgaa atgaaggaga agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt
    21901 tctgccggac gtgcttcttc tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt
    21961 aatgctctgc atggtgcagt gattaatttc attgtgttaa tccatgagct aaagaaaaag
    22021 tacagccttt tatcgttgta attataaaaa tataaaggca gaaactattt tccctctcct
    22081 catccaggaa gagggaaaat gcctgctcat tttttattag tctgcttaat caacattgct
    22141 ggctggcgtc gcttcaatat ggcgctcttc agccgttaat ccctttatat atttgctgta
    22201 tttccaccac gcaaagccga ggaagataac aataccgccg acgttataaa aaatgatggt
    22261 cagaatattc gcgccagtcg ggaatgtcga agcgacaaac cctacggcaa atatcgcaat
    22321 cagcattgaa acaacgataa taccggtgcg gcgggagccc atacggaaat cgcgtggcag
    22381 gtgatctaat ttggcgcgta aattcaggta agccagcatg atgaataacg gcggaagcat
    22441 ggacgctgcg gcggtcatat taataatggt attcatcaga tcctgcacgg tattggaacc
    22501 gagcatcggg ataatcatca gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc
    22561 cggaacgccg ttttcgttaa gttcgacggt tttcttacca aagatgcctt ccgggatttc
    22621 ggagaagaaa attttcaccg gcgttgcggt ccacatcagc agggaaccga acattgcggt
    22681 aaaggacacc agaccgacaa agcgattcat caacgcttcc ggcagaccaa aatacgccgc
    22741 catgccgtgg aatacctgca ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa
    22801 gacgttaatc agcaccgagg agacggaata cagtacgccg ataaaaatcc cggcgaggat
    22861 gatcacttta acgaacgatt tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga
    22921 ctccgcgcca cctgcggcca taaagatcca ggtggtaacg ccgaggaacg cccagttgaa
    22981 gttcgggatc atcgcgtcaa cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc
    23041 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
    23101 ggtaattggc cccagcattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
    23161 caccatactg ataacggtgg tggcaaccgg cgtcataata tattcgtagc cgaggaaggc
    23221 atacgaagca taagcaataa cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa
    23281 ccagtaagtg taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccacgc
    23341 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
    23401 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc
    23461 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
    23521 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgtgt
    23581 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
    23641 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
    23701 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctctac ggtttctccg
    23761 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
    23821 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
    23881 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtcat tttccacacg gtaggtcaat
    23941 gaatcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
    24001 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
    24061 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
    24121 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
    24181 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
    24241 tggagccgag gccaagtagc tggtggtcga ggttcagggt gatgtcatca ctgcgctgca
    24301 gctcgttaca gtgctgggca gcgtggatgt tttcctgggt atagtgccag gcgctgaagt
    24361 taattgggcg ctgcggaacc accagcagac cgttgccgtg gcggttagtc agtgccgtcc
    24421 agcggacatg ctgacggttg ccgttgttct gcgggaaggg atagttctcg aacatggcat
    24481 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
    24541 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaatcccc atggtgaaac
    24601 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
    24661 cctggccatc ggcagcgatg cgccagatgt aggtacagcg catcccgaag tcaaacaccg
    24721 gcggtgcaat aactgtgcgg ctgatgatca acacttcacc atcgctctgt tctacggcaa
    24781 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
    24841 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
    24901 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
    24961 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
    25021 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
    25081 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
    25141 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
    25201 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
    25261 cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
    25321 actcgccgcg agtcagcgcg tggattttta ccggcgcgat aacctgtttg tactctttca
    25381 ggcccgatcc cggcgtctga tcggaataga tcaaaccatc aagacagaag ttatagttgt
    25441 tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg tcgtcctgtg
    25501 cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa tcgtgcttat
    25561 agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca tgagcatatt
    25621 cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc agcggcacgc
    25681 gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct tcgtaatgaa
    25741 ccagtcgcgt gtcatccagc gctttcgccg catggtacat cgcgcggatg ttacagccat
    25801 agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc tgtgcgtgga
    25861 tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg gtaatacggc
    25921 tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc atcacaaaca
    25981 ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga gcggtacgca
    26041 cggagttgat attgtgctgc ttcatcaact ggagatcttt ctcgacgcga tccattccaa
    26101 cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc atcacataac
    26161 ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca acgcgttgtg
    26221 gcaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga tggtaaagat
    26281 aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag ctggcgctgg
    26341 tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt tcgccatcaa
    26401 acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc agcaccactt
    26461 cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcaca gtgaaatcgt
    26521 taatatgcgt taggtgtttt ccgaccagat aaacatcgcg gaagatcccc gccgaccacc
    26581 acatatcctg gtcttccacg taggtagagt ccgcccactg catcacgcgc acacacaaca
    26641 ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg cgactgccct
    26701 tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg acgccgtcaa
    26761 atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata cgttgatagg
    26821 caccggttgg gttatcgctg gggacaaacg gcacatcgat ggggaacgga aaaccttcgt
    26881 cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg gtaatatgcc
    26941 cccagtcagc cattaactca gaggtgaagg cttctggtac ttgcagcgga tggtcaaaaa
    27001 agtggaaatt ccactgactg cttaagggca gaaacaggct gctggtttcg cgggcaaagg
    27061 tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgcggcgca agtcggtttt
    27121 cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga gagaaatcgg
    27181 aaccattcat ggtgatggta gtttgtgtaa ttgctggggg tttagtaaaa tttttactaa
    27241 aaagcagcag gtaaatgcat ttactttaac tggatcacga aaataaacgg cagcactgtc
    27301 cggcaaaaat gattgccgaa cagtgtgtaa ggggatttaa cgggtcgtgc cgcgcagttt
    27361 taatttgctg ggaacgaaga ctagcagcgg cagcgcgcga ccatcgcggg ctttttcata
    27421 caccaggtta acgccctgac ttcccatcat ctcggaatgg atgcgcacgg tggagagcgg
    27481 cggaaaggta aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa tatcttgtgg
    27541 gatgttcagg ccacgttcat gaattgcccg cagtacgccg atagcaatgg aatcggaagc
    27601 aacaaacagt gccttcggat agtcttcccg cgccagcatt tgttttgcca gttcataacc
    27661 cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg cgtaccactt gtttcagtcg
    27721 gccatattcc gcaaaggcga cctcacgaat atccgccttg ccaggctcat cttcaccgcc
    27781 aataaaacca atacgattaa cgccctggtt gatatagaag tcgatgattt ctttactgat
    27841 gcgtgccaga tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa agtcgataaa
    27901 acagatattg tcggtcaacg cgctggcagc ggcgcgcagg gcgggcgtgg gtttgccgac
    27961 aattaaaata ccggtgacgt ttttaatgtc tggtaatccg ttgtgttcat aacagttggt
    28021 cagttcgatc gccagctttt cgcactgagt ttcaatgccg tggcggatcg ccagatagta
    28081 aggatcgttg atctccagct cctgctggta gctgtagata gccagaatat ggtgttggtt
    28141 gactgcgcct gtctgtagtt tacgggcgct actggtcttg tactccagct tttcggcgat
    28201 ctcgagaatg cgatgtttcg tctcttcttt cacattcaat gtcggatcgt catttaagac
    28261 cctggatact gtcgccaggg atacgccagc ttcgattgcg atgtctttta atgttgccat
    28321 gtttaccttt tttactttcc ctgatgtgcg cccccattgt agaggaagtg cttatcgggt
    28381 cacctgtttt cagtaaataa agcgcgtttc tttgacgctg taacggtaac acagcggctt
    28441 aggaatgtga tcacgcctgc attctccatt tttagcctac aggattgaga aaaacttttc
    28501 tatagtttag taaaatttta ctcgaaggga ggaagcaatg gaaaccgtgg cttacgctga
    28561 ttttgcacgt ctggaaatgc gcgtcggaaa gattgtggaa gtgaaacgcc atgaaaacgc
    28621 cgacaagctg tacatcgtac aggttgatgt ggggaaaaaa cactgcaaac cgtgaccagc
    28681 ctggtgccgt actacagcga agaggagctg atggggaaaa cggtggtggt cttatgcaat
    28741 ctgcaaaaag cgaaaatgcg tggtgaaacg tcggaatgca tgctgttgtg tgcggaaacc
    28801 gatgacggca gtgaaagcgt gttgttaacg ccggagcgga tgatgcctgc gggcgtacgc
    28861 gtcgtataaa aagatcggat ggcgacgtcg tatcgccatc cgatttgata ttacgcttct
    28921 tcgacactta ctcgcatggc cgccagcgcc ttacgcgccg ctttgatcac cagttcacac
    28981 tgttcaatgg tcagtgtcag tggcggttca atgcggatcg ttttggcgtt attgagcgtt
    29041 ccggccacca gtacgcgctg gcggaacatc tcgctggcaa agttatagcc gatttcgtta
    29101 tcaacaaact caatcgccat caacatccct ttaccacgcg cttcctgtac cagatcggga
    29161 tattcccgcg ccagttgacg gaaaccgtcc agcaacatat cgcctttttg ctcagcctga
    29221 gccggtaagt tctgctccag caacacattg atggtcgcca gcgccgccgc acaggccagc
    29281 gggttgccgc caaaggtggt ggtatgcagg aatgggttgt cgaacagaac tgaaaacacc
    29341 tcttcagtgg cgatggtcgc gccaatcggc atcacgccgc cgccgagggc ttttgccaga
    29401 cacagaatgt ccggctgaac gttctcatgt tcgcaggcaa acatcttgcc cgtgcgcccc
    29461 atgcccgttt gtacttcatc gaggatcatc agtgcgccga actcatcgca cagcttacgc
    29521 acggcggtga gatagcccgg cggcggcaga attacgccac cttcgccctg aatcggttcg
    29581 aggatcaccg cagccacatc atcaccggtt tttttgcact cattaagagc cgtgcgcatg
    29641 gcttcgatat tgccaaacgg cacatgacgg aaccctggca gcagtggcat aaacggtttg
    29701 cggaaggtcg atttcgccgt ggccgacagc gcgccaagtg atttaccgtg gaacacgccg
    29761 ctggtggcaa taaaagtaaa tttgccgcgc ggcgactgat aagccttcgc cagcttcagc
    29821 gccgcttcaa cggactcggt gccgctatta cagaagaagc tgtatttcag tttaccgggc
    29881 gttagcgcag caagggtttt cgccagcata gcccgtaacg gatcgagtag ctcctggctg
    29941 tgcagtggtt gtttcgcaag ttgattctgt acggcggaaa ccacaactgg attacggtgc
    30001 cccacgttga aaattccaaa acctcccagg cagtcgataa actcctgtcc ctgggtgtcg
    30061 acaagcgtat ttaaacctcc cgcttgccac tctacggctc cgtaatcccc gccggcggta
    30121 acagatttgc gatactctaa aaaccccgga ttgacatgct ctttgaagta ttcaatcacc
    30181 tctcggttaa gtgctttcat ctcctcatga tccagcgttc gcttctcaat gagattcagg
    30241 gcgtgggcgc tgcacgctaa agccgatgcg ctcgaaggta acctgttcaa aatatgctcc
    30301 ggaggctcgc gtatcacatg ataccgattt aagtattgca gggattgcgc cactccggct
    30361 gcgatcgcga aaatcgggat aaacacaaat gcggttatca tttgtgcaat atttaatgct
    30421 atccgttaac atttgttgtt attttgcacg gtttttgtcg ggtatggatt tgatgattgc
    30481 tgcgcaaatg cacgttttca gggcaataat tgcctgattt ttgggcaaga taattaacgt
    30541 tttcgtatag taacagagtg aaagtatttt aaatcaaaga attagatttg tgctttaatc
    30601 gcaaattgcg atctaaatca aattaatcgg ttaaagataa ccgcagcggg gccgacataa
    30661 actctgacaa gaagttaaca accatataac ctgcacagga cgcgaacatg tcttctcatc
    30721 cgtatgtcac ccagcaaaat accccgctgg cggacgatac cactctgatg tccactaccg
    30781 atctgcaaag ctatatcact catgctaatg acacttttgt gcaggtgagc ggctttacct
    30841 tgcaagagtt acaagggcag ccgcacaaca tggtgcgtca cccggatatg ccaaaagcgg
    30901 cgtttgcgga tatgtggttc accctgaaaa aaggggagcc ctggagcggc atcgtgaaaa
    30961 atcgccgcaa aaatggcgac cattattggg tgcgggccaa tgcggtaccg atggtgcgcg
    31021 agggaaaaat cagtggctat atgtcgattc gtacccgggc gacggatgaa gagattgcgg
    31081 cggtggagcc gctgtacaaa gcgttgaact ccggacgtac cagtaagcgt attcataaag
    31141 gcctggtggt gcgtaaaggc tggctgggta aactgccttc attaccgctt cgctggcgga
    31201 cgcgtggagt gatgaccctg atgtttatct tgctggcggc catgctttgg tttgttgctg
    31261 ccccggtggt gacgtatttc ctctgtgtgt tagtggtatt gttggcaagc gcttgttttg
    31321 aatggcagat tgtgcgcccg atagaaaatg tcgcccgtca ggcactgaag gtggcgaccg
    31381 gagagcgtaa tagtgttgag cacctgaatc gcagcgatga gctggggctg acattacgcg
    31441 cggtagggca gcttggcctg atgtgccgtt ggctaattaa cgatgtctca agccaggtgt
    31501 ccagtgtcag aaatggcagt gagacgctgg cgaaaggcac cgatgaactg aacgaacata
    31561 cccagcagac agttgataac gttcagcaaa cggtggcgac catgaaccaa atggcggcgt
    31621 cggtgaaaca gaactctgcc acggcgtcgg ctgccgataa actttctatc accgccagta
    31681 atgcggcagt gcagggtggg gaggcgatga ccacggtgat caagacaatg gacgatatcg
    31741 ccgacagtac ctagcgcatt ggcaccatta cttcgctgat taacgatatt gcgtttcaga
    31801 ccaatattct ggccctgaat gcggcggtgg aagcggcgcg tgccggcgaa cagggcaaag
    31861 gttttgcagt ggtggcaggg gaagtgcgtc atttagccag ccgcagcgct aatgctgcca
    31921 acgatattcg caagctgatt gatgccagtg ctgataaggt gcaatccggt tcgcagcagg
    31981 tacacgccgc cggacggacg atggaagata ttgtggcaca ggtgaaaaac gtcacccagt
    32041 tgatcgccca gattagccat tcaacgctgg aacaggccga tgggctttcc agcctgaccc
    32101 gtgcagtgga tgagcttaac ctgatcaccc agaaaaatgc cgagctggtg gaagagagtg
    32161 cgcaggtgtc ggcgatggtg aaacaccgcg ccagccgact ggaagacgcg gtgacggtgc
    32221 tgcattaatc ggtatgccgg atccggcggt tggagcacaa tgcctgatgc gatgctggcg
    32281 catcttatca ggcctgcggg gtgtgtagcg ggtgtaggcc ggataaggcg cgtcaagcgt
    32341 cgcatccggc aattgcaccg cgccactggc ggatgcggcg attaatccag ttgcgtaata
    32401 tcaaaagcgg cgcggtcgat caccgcaatg atctttttta tttgtgcatc actgatatcg
    32461 ctctggttga cgcgtaaatc cagcaccgct ttaaaattat ccagcgcccg cttcatttgc
    32521 gggttctggc gcagcgccgc gccaacgcaa cgcgctttga tgcgttcttc aatcatctcc
    32581 acttgttcgc ggttttcttc cagccactgc gcgccttgtt cggtcagcgc aatctgcttc
    32641 ttaccacctt cctcttcgcg gatggtaatc agcgactgct cctgcagaaa atccagcgtc
    32701 gggtagatga cgcccgggct tggggtgtaa ttcccctggg ttagattctc aatcgcttta
    32761 atcaattcgt aaccgtggct gtcatcgcgc gagagaatat ccagaatcac cagacgcaat
    32821 tcaccgtgac caaagaaacg ttgccgacga ccgccgccgc gaccgtggcg accaccgcaa
    32881 cattgaccgt gttcatgctg gtgaccgtgt ccgcagtgct cgtgttctga cttctcacct
    32941 ttgcagcagc cctcatggcg tggctggcct tcatgtttac aacacccttc gtgatgatgg
    33001 ctcatatcag cctccttttg cttgattaga tatatctaat ttatatctga tgtgagcatt
    33061 actgcaagta caaaaatgat ttttaaacgt ggaagcacct cacatctagg ccggataaga
    33121 cgttcacgcc gcatccggca gcattgctat ggcactgaat gccttcacat acttcagaca
    33181 tatcagttct acaaatcgct tgcatttatc atgattaaca atcattatca tttgcgaatt
    33241 ttatttagat atatctgatt accatcacga aggcgataac aatgaataac accccccgct
    33301 acccgcagcg cgttcgcaat gatctgcgct tccgtgaact gactgtgcta cgcgttgagc
    33361 gcatcagcgc cggttttcag cgcattgtcc ttggcggcga ggcgttggac ggttttacat
    33421 cgcgcggctt tgacgatcac agcaaactct tctttcctca acctgacgct cactttgtgc
    33481 cgccaacggt aacggaagag agcatcgtct ggccggaagg accacgccca ccgtcgcgtg
    33541 actatacgcc actgtatgac gaactacgcc atgaactggc gatcgatttc tttattcacg
    33601 acggtggggt cgccagcggc tgggcgataa acttacggtg gcaggtccgc gcggttcgct
    33661 ggtggtgccg gaagattacg cgtatcagct gtatgtctgc gatgaatccg gaatgcccgc
    33721 actgcgccgc cgcctggaaa cgttgagcaa acttgccgtt aaaccgcaag ttagtgcgct
    33781 ggttagcgtg cgggataacg cctgtcagga ttatctcgcg caccttgatg gttttaatat
    33841 cgaatggctg gcacatgatg agcaggcggt agatgcgcgt ctggcgcaga tgcaaatccc
    33901 tgccgatgat tacttcatct ggataaccgg cgaaggtaaa gtcgttaaga atttaagccg
    33961 ccgctttgaa gcggaacagt atgacccaca gcgggtacgt gcagcggctt actggcacgc
    34021 aaagtaactg atatataaaa agaaaaaggc tgacgatttc tcgtcagcct ttgctatatc
    34081 tggtggcccc tgctggactt gaaccagcga ccaagcgatt atgagtcgcc tgctctaacc
    34141 actgagctaa ggggccatgg tagcggatta taaagtaact ccgtgccgca atccagccat
    34201 taccgcgcgc ctgctgtttt tataaacaat gtattttcaa tccgttatac ttttcctggt
    34261 gatgttagaa aggagtaaat atggttgagg atattttggc tccagggtta cgggtcgtgt
    34321 tttgcggtat caaccctggg ctttcatccg ccgggactgg ttttcccttt gctcatccgg
    34381 caaatcgctt ctggaaggtg atatatcagg ccgggtttac cgaccgtcag ttgaagccgc
    34441 aggaggcaca gcatctgctg gattatcgtt gtggcatcac caaactggta gaccgtccaa
    34501 cggtgcaagc caatgaagtt tcaaagcagg agctgcacgc aggtgggcgt aagctgattg
    34561 aaaaaattga agattatcag ccgcaggcgt tggctattct gggcaaacaa gcatatgaac
    34621 agggattcag ctagcgcggt gcacagtggg ggaaacaaac gctcaccatt ggttcgacgc
    34681 agatttgggt gctgccaaat cccagcggtt taagtcgcgt ttcactggag aaactggttg
    34741 aagcgtatcg cgagctggac caggcgctgg tagtgcgtgg gcgataaaaa acgtcaccgg
    34801 ggg
//
LOCUS       DAJTUM010000035        34445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTUM010000035 DAJTUM010000000
VERSION     DAJTUM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..332)
                     /locus_tag="OO471_001820"
     CDS             complement(<1..332)
                     /locus_tag="OO471_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243109.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO471_001821"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO471_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6243110.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO471_001822"
     CDS             complement(1693..2073)
                     /locus_tag="OO471_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6243111.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO471_001823"
     CDS             complement(2087..2503)
                     /locus_tag="OO471_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243112.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO471_001824"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO471_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6243113.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO471_001825"
     CDS             complement(3504..3971)
                     /locus_tag="OO471_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6243114.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO471_001826"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO471_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6243115.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO471_001827"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO471_001827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6243116.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO471_001828"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO471_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6243117.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO471_001829"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO471_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6243118.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO471_001830"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO471_001830"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6243119.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO471_001831"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO471_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6243120.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO471_001832"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO471_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6243121.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO471_001833"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO471_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6243122.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO471_001834"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO471_001834"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6243123.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO471_001835"
     CDS             complement(14626..15441)
                     /locus_tag="OO471_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6243124.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO471_001836"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO471_001836"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6243125.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO471_001837"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO471_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6243126.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO471_001838"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO471_001838"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6243127.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO471_001839"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO471_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6243128.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO471_001840"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO471_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6243129.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO471_001841"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO471_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6243130.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO471_001842"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO471_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6243131.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO471_001843"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO471_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6243132.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO471_001844"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO471_001844"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6243133.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO471_001845"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO471_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6243134.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO471_001846"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO471_001846"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6243135.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO471_001847"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO471_001847"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6243136.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO471_001848"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO471_001848"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6243137.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO471_001849"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO471_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6243138.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO471_001850"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO471_001850"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6243139.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO471_001851"
     CDS             31575..32189
                     /locus_tag="OO471_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6243140.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO471_001852"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO471_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6243141.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO471_001853"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO471_001853"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6243142.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8699 c         9302 g         8336 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc cttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaataccc
    34441 cgcgc
//
LOCUS       DAJTUM010000036        33589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTUM010000036 DAJTUM010000000
VERSION     DAJTUM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            574..711
                     /locus_tag="OO471_001854"
     CDS             574..711
                     /locus_tag="OO471_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243143.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1311..2090
                     /gene="ipaJ"
                     /locus_tag="OO471_001855"
     CDS             1311..2090
                     /gene="ipaJ"
                     /locus_tag="OO471_001855"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6243144.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3037..3966)
                     /gene="virB"
                     /locus_tag="OO471_001856"
     CDS             complement(3037..3966)
                     /gene="virB"
                     /locus_tag="OO471_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6243145.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4183..4419)
                     /locus_tag="OO471_001857"
     CDS             complement(4183..4419)
                     /locus_tag="OO471_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6243146.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4423..6324)
                     /gene="ipaA"
                     /locus_tag="OO471_001858"
     CDS             complement(4423..6324)
                     /gene="ipaA"
                     /locus_tag="OO471_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6243147.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6333..7331)
                     /gene="ipaD"
                     /locus_tag="OO471_001859"
     CDS             complement(6333..7331)
                     /gene="ipaD"
                     /locus_tag="OO471_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6243148.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7382..8473)
                     /gene="ipaC"
                     /locus_tag="OO471_001860"
     CDS             complement(7382..8473)
                     /gene="ipaC"
                     /locus_tag="OO471_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6243149.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8493..10235)
                     /gene="ipaB"
                     /locus_tag="OO471_001861"
     CDS             complement(8493..10235)
                     /gene="ipaB"
                     /locus_tag="OO471_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6243150.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10241..10708)
                     /gene="ipgC"
                     /locus_tag="OO471_001862"
     CDS             complement(10241..10708)
                     /gene="ipgC"
                     /locus_tag="OO471_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6243151.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10765..11391)
                     /gene="ipgB1"
                     /locus_tag="OO471_001863"
     CDS             complement(10765..11391)
                     /gene="ipgB1"
                     /locus_tag="OO471_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6243152.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11407..11796)
                     /gene="ipgA"
                     /locus_tag="OO471_001864"
     CDS             complement(11407..11796)
                     /gene="ipgA"
                     /locus_tag="OO471_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6243153.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11809..13293)
                     /gene="icsB"
                     /locus_tag="OO471_001865"
     CDS             complement(11809..13293)
                     /gene="icsB"
                     /locus_tag="OO471_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6243154.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13607..15223
                     /locus_tag="OO471_001866"
     CDS             13607..15223
                     /locus_tag="OO471_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6243155.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15233..15595
                     /gene="ipgE"
                     /locus_tag="OO471_001867"
     CDS             15233..15595
                     /gene="ipgE"
                     /locus_tag="OO471_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6243156.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15595..16053
                     /gene="ipgF"
                     /locus_tag="OO471_001868"
     CDS             15595..16053
                     /gene="ipgF"
                     /locus_tag="OO471_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6243157.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16062..17177
                     /gene="mxiG"
                     /locus_tag="OO471_001869"
     CDS             16062..17177
                     /gene="mxiG"
                     /locus_tag="OO471_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6243158.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17185..17436
                     /gene="mxiH"
                     /locus_tag="OO471_001870"
     CDS             17185..17436
                     /gene="mxiH"
                     /locus_tag="OO471_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6243159.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17449..17742
                     /gene="mxiI"
                     /locus_tag="OO471_001871"
     CDS             17449..17742
                     /gene="mxiI"
                     /locus_tag="OO471_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6243160.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17748..18473
                     /gene="mxiJ"
                     /locus_tag="OO471_001872"
     CDS             17748..18473
                     /gene="mxiJ"
                     /locus_tag="OO471_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6243161.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18470..18997
                     /locus_tag="OO471_001873"
     CDS             18470..18997
                     /locus_tag="OO471_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6243162.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18939..19634
                     /gene="mxiN"
                     /locus_tag="OO471_001874"
     CDS             18939..19634
                     /gene="mxiN"
                     /locus_tag="OO471_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6243163.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19627..20034
                     /gene="mxiM"
                     /locus_tag="OO471_001875"
     CDS             19627..20034
                     /gene="mxiM"
                     /locus_tag="OO471_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6243164.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20018..20446
                     /gene="mxiM"
                     /locus_tag="OO471_001876"
     CDS             20018..20446
                     /gene="mxiM"
                     /locus_tag="OO471_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6243165.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20576..21208
                     /gene="mxiE"
                     /locus_tag="OO471_001877"
     CDS             20576..21208
                     /gene="mxiE"
                     /locus_tag="OO471_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6243166.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21195..22895
                     /gene="mxiD"
                     /locus_tag="OO471_001878"
     CDS             21195..22895
                     /gene="mxiD"
                     /locus_tag="OO471_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6243167.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22914..23981
                     /gene="mxiC"
                     /locus_tag="OO471_001879"
     CDS             22914..23981
                     /gene="mxiC"
                     /locus_tag="OO471_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6243168.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23994..26054
                     /gene="mxiA"
                     /locus_tag="OO471_001880"
                     /gene_synonym="virH"
     CDS             23994..26054
                     /gene="mxiA"
                     /locus_tag="OO471_001880"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6243169.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26067..26468
                     /gene="spa15"
                     /locus_tag="OO471_001881"
                     /gene_synonym="spaK"
     CDS             26067..26468
                     /gene="spa15"
                     /locus_tag="OO471_001881"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6243170.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26472..27764
                     /gene="spaL"
                     /locus_tag="OO471_001882"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26472..27764
                     /gene="spaL"
                     /locus_tag="OO471_001882"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6243171.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27857..28195
                     /gene="spa13"
                     /locus_tag="OO471_001883"
                     /gene_synonym="spaM"
     CDS             27857..28195
                     /gene="spa13"
                     /locus_tag="OO471_001883"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6243172.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28182..29060
                     /gene="spa32"
                     /locus_tag="OO471_001884"
     CDS             28182..29060
                     /gene="spa32"
                     /locus_tag="OO471_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6243173.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29054..29935
                     /gene="spa33"
                     /locus_tag="OO471_001885"
                     /gene_synonym="spaO"
     CDS             29054..29935
                     /gene="spa33"
                     /locus_tag="OO471_001885"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6243174.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29940..30590
                     /gene="spa24"
                     /locus_tag="OO471_001886"
                     /gene_synonym="spaP"
     CDS             29940..30590
                     /gene="spa24"
                     /locus_tag="OO471_001886"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6243175.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30605..30865
                     /gene="spaQ"
                     /locus_tag="OO471_001887"
     CDS             30605..30865
                     /gene="spaQ"
                     /locus_tag="OO471_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6243176.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30865..31635
                     /gene="spa29"
                     /locus_tag="OO471_001888"
                     /gene_synonym="spaR"
     CDS             30865..31635
                     /gene="spa29"
                     /locus_tag="OO471_001888"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6243177.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31642..32670
                     /gene="spa40"
                     /locus_tag="OO471_001889"
                     /gene_synonym="spaS"
     CDS             31642..32670
                     /gene="spa40"
                     /locus_tag="OO471_001889"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6243178.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32683..32925
                     /locus_tag="OO471_001890"
     CDS             32683..32925
                     /locus_tag="OO471_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243179.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10475 a         4883 c         6731 g        11500 t
ORIGIN      
        1 atttcaggcg ttgggtgtgg atcactattg caccgttcgt gacacttgtt gactacctgt
       61 gcggcatctg gtatcaaggt ggcgtgaagc cggttcaggt tctcatgcgg aacgttggaa
      121 cctatatccc cccgatacca aaggcgaaat gtaagtgaca aaatgcaatg aacacgttct
      181 ccgttaactg aggagcatgg tgataaataa aggtaaatgc agaaggtgta gagtctcgca
      241 gatttctgac atcatagagc ctaacattag atgcataacg ctacagattg tagaatctct
      301 acaggtgaaa tatacactgg ttggcgttta tgtcgcaaaa catgtcattc aggttcatct
      361 caccaataag gatatgagtg aagtggagga taagtgagtc tgctgtcaga gttttctggt
      421 gtatgtcagt aactgcgaac catgtctgct tgatatggga gcttatggag gctctctgta
      481 caaggttagg gagctaacaa agcagggata aaaagtccgc ctgatgcagg cacggttcgt
      541 taaatctttt gccattggta acaaaatggt gcgatgaatg ttcaggctat ctttatcttg
      601 atggtggttc agctgtttgg taaagaaatc gctgtaacaa tagaagaact gcaggcagta
      661 ctggtcccac atcgtttgct cagtcaactg gtgaagttcc gtagcgcgta aattaatgct
      721 ttgcatgaaa cgttactgga gtggatgaaa gaatacctgc atatatcatt actgcgttgg
      781 attaccagta caacagattg aatgaactgg acatgttgat agacggcatt gaaaagcagc
      841 ttataagcat agtgcggcag agttaattgg agagcacttc gccttctgag tattaagtaa
      901 gcatggtggt acctgcggat actatctatt cactgtgctc ggtcggcagc cttactgacc
      961 aaggagttgg gggagttgat aacaaagttg aaaaaacagc tctcaaccaa ggtggttatt
     1021 gtaacaatag caaataagct ggtgtgacct gtatcacaat aaccgattat ggacgtaaat
     1081 acgacaaaaa tatgtcagta tcaggcggta ttaattgctg atgctgatgg acattaactc
     1141 ttaataaaag ggtggatgct gaaaggttgt tatggtgacg gacgtgatga caacggaaaa
     1201 gagtatgagg ttttggattt aaagttcgtt cggggaataa ggacccggtc agctaattct
     1261 catagtagtt cgcattgctg gatgcaatac tgtgctgcaa ttactcgtcg atgtcggaac
     1321 aacggaagcc ttgcaaacgg ggatgtattc atacaggtgt tatgttatat ggggttttat
     1381 tgcagggggc tataccaaga gaatatatga tatctcatca gactgatgtg agggtaaatg
     1441 agaatagagt aaacgaacaa ggttgttttt tggcgaggaa gcagatgtat gacaattctt
     1501 gtggagctgc cagtttactc tgtgctgcaa aggaattagg ggttgataaa ataccacaat
     1561 acaaagggtc tatgtccgaa atgacaagga agagttctct tgatttggat aatcgttgtg
     1621 agcgggattt atacttaata actagtggta attataatcc gagaatacac aaagataata
     1681 tcgcggatgc cggttattct atgcctgata agatagtcat ggctacgagg cttcttggat
     1741 tgaatgctta tgtcgttgaa gaaagtaaca ttttttcgca agtaattagt tttatttatc
     1801 ccgatgcaag ggacttatta attggcatgg gatgtaatat agtacatcaa cgagatgtgt
     1861 tatcttctaa ccaaagagtt ttggaagctg ttgctgtatc ctttattgga gttcctgtcg
     1921 gcctccactg ggtgttgtgt agacctgatg gaagctatat ggatccagct gtaggagaaa
     1981 attattcttg tttttcaact atggaattag gtgcaagaag aagtaattct aactttatag
     2041 gatatactaa aataggtatt tccatagtta taactaatga ggctttgtaa attgtcgttt
     2101 tttatctgtg aaggagcttg aatattttgg gggggggggc ataacaaatg atattactga
     2161 gtacaaattt agctatattg ggcggattag ttgtgtggga agacgaaatg tttctcttga
     2221 aataagtgaa ctccctcaag gaatgattgt gtgcttaaat atttaagtgc tggtaagttt
     2281 gagaaggctg acatattttg cagagcaaac agtgattgac ttggatgata tttttatttt
     2341 agtcactcgt tgtaattgga gtatgcttta ttttatttga aaatagatgt attttatacc
     2401 cgcaatttaa actgtaagcc ctatgagggg tatggatatt ataagttact ctttgaccaa
     2461 gcataacttt tggggttaga aaaataaatc tcttgttaat aattaatatg attttaaaat
     2521 caaacttgtt tataattata aaataatgta agtcggtcat tttatgggaa gattaggaga
     2581 ataaaacagt aattatattt gagaaatctg ctaatatatt cttaaagctt ttttgatact
     2641 gtgaacgaag tctgttaatg gtggattttt tgtttatcac catgtaaaaa aaattgagta
     2701 gtaaaagcgt tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa
     2761 tattttcggc cagtcactca tattcacata aaattttgac catcatgccg catcccttag
     2821 ttgcacaatt cattttcaat gatatgaata ttactaattt caccagataa ttttctcgtg
     2881 gtcagcaggg tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat
     2941 aacagatagc cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat
     3001 tatctgaatt gggcagttta catcagtgtt cgatgtttat gaagacgata gatggcgaga
     3061 aattatatcc cgaatagctt catctatttc tctctgaact gttttgttta ttttagataa
     3121 tgtaaatatt agagttctgt ttgttttatt ctcttttctt tttatgtaag tccgtttgtc
     3181 tttagaaata aataacgagt caacttttag cgaagggttt tgctttctgt ttttgctttt
     3241 ctttatgatg tttaaaattt cttttttata tatgtccggg ggcaaatttg tatcaaggtc
     3301 ttttatttct tcctttaata ttggtagtgt agaactaaga gattcattag ccttttcaag
     3361 tcctttataa taattgaata atatcttgta gtcattaaag ttaagttctg acgcgattgg
     3421 aaatagagat attatttcct gtggaacgct tgctgcctga aaggcacgag tgactttcgc
     3481 gcgagacaga ttctcttttt tggctatgtc tttataggac atccctgata ctttcagaaa
     3541 attaagacca ataccaagtt ctcggatgct atgctctttt gctgtttgta tatcgtttgc
     3601 tagttttctg gcatcaagag tagatatata ttcttttgaa tatagaactt caagatctgc
     3661 tcctgcatat attgcagatg ctcttctacg agtgccatcc agaatttcaa ttctaccatc
     3721 aatctccctt cctattacag ggaagaattg ttgtagcttt atagttttta tgatatcggc
     3781 taatgattct tctgttaggg attcttgatc gcgtacattc gttttttggt tgacgaaggt
     3841 taaatcttgg atccttttat gaggaatctt ggctttgata aaggaaactt tattaccaga
     3901 atggagtgta aactctttct tttggccttc ctttatactt aacaagtcgt tgcacaaatc
     3961 caccatatca caccctgttt attcatattg atttatattg acaaaagtta aatgcagtgt
     4021 gagcaagatt gtatgtaact gttgatgata gtcaacggta gtcttaaaaa ttactatttt
     4081 aatagctgga ttgatgcaat ttatctttat tggataaaag aataatattc ttgtggagct
     4141 ctgatgtgag agctccatag atgaaaaaat gcttatcaat tactatattg attttgtact
     4201 atttttgact acagaaataa gatctccaat agtaataatg tgttctaatg tagattcatc
     4261 tatacgtaga ttgaactcaa aactaatcat atccagcatt tcaatcagag aaagagaatc
     4321 ggcagaaaga tcttcaataa atttagtttc ttcagatagt ttgctgtacg ctataccata
     4381 gcaaaaggcc actattgata atattttttc ttttatcata ttttaatcct tattgatatt
     4441 ctttaatact tttgataggg aagtggttac atcttttgct gctttatata tattattgtt
     4501 ttcagctgta atatcattgt tattttttaa atcagatata tcatctgtaa gtaattctgc
     4561 agaggtatcg tcaatttttg acaatacctt gctgagggcg ctagatactt cttttgcctt
     4621 ttcatagatc gcatgatttt tatcaattgt atctgcgttg gtttctgtta cgtagtttct
     4681 tatatttgca tctgtaactt ttaatttgtt gtttattcta tgttctgttg tggattttgt
     4741 aatgtccttt gatttttcct gtaactctcg ttcttgtgtt cctgatttag ttccaatgag
     4801 agatataaga ttacttaggg agtttgttac tttttttgaa gcttcaaata tcgtttcccg
     4861 tgttaaatat gtgtctccat ttcttgataa aaaatcagtc tgaagtactt ttgaatcagt
     4921 gtttgagttg aatttattga tttgcgaagc tactctatga gaaagagaag cgctactttt
     4981 agttgtatct gatgagttat cattactgct tgtcactcct tccgggtgga gagggggggt
     5041 ggttgaagag ttctgtatgt tcagaggcct gtgtccccga gaaagagagt tatttgcaga
     5101 tctttcttga ctactcgtag ttaacaggtc tgaacctatt ccatccgaat tatctggagt
     5161 atggttgaaa gccttttcta aagctgaatt atttgcaaat gtctggacag tatctgatat
     5221 agagtgtggt agaacggaag ttactttttg aactagtgct ctttgtggat ttagtaatga
     5281 gtttccgtta cgggataata gatcgtttgt ctgagaagta ggagactgtg catcattttc
     5341 tgggttctcg tgatagctat tgtcatatac tctgttgtca aaaactctat tatcgtaagt
     5401 tctattatca ttatttatat gatagtgaat tacaggttgg gatgttttct cattggctcc
     5461 gggggttggt ccgccatctg gcatatcttc gggaggtgta gggaggccat cttggatagg
     5521 atgcgaatct tttatatctt ctaaaatatc attaaggact tttctcaaca gctcaggaaa
     5581 cacggctact gtatttaagt ctcttgacag tttacctata ccaaatcctt tactatcaat
     5641 agtattttta cgttgattgc gcaaactatc aaaggcaagt tttgcctgtg cgtcaactaa
     5701 atcatctagt ttgagagatg aaacagtttc attacttaac tttaattcag caagaatatt
     5761 attcttgata aatgatgata cttcagtatt gaggagtttt tgtcccaatg ataaagcttc
     5821 accagatgtg tgttgttgtg ctaatagtgc aagtctgtgc tcaatgtttt gtcgtaattg
     5881 atggagtttc ttattctttg ccgaccatgg tccaaaggca gaatcttggg ataatttatc
     5941 ttttataatt agttccgaat actgtgcgac aattttttga agtccctttt cgttttcaaa
     6001 attaagacca ttttgatgta ctaaggagta caaaacattt gaaaattcga gaatctcgct
     6061 tttggcatac gcattcattg agttggaata aatgcttctt aacacatctt ctttccctga
     6121 ggaagaaaat aaaaaatcaa gacatttctg attaaaagac gttgcaaagt tttctttttc
     6181 agacactgat gctggtgttt ttagagaagt ttgcgaacta tggatatcgg atattttgct
     6241 ttttaactcg ctgtattctg ttgatgatgg tgaagttgcc ttatataaga atgttggcgc
     6301 ttgagtatta tttacattat gcatggcgca cctcagaaat ggagaaaaag tttatctgta
     6361 tctgtacatg agcttattgt actactcaaa acctttacta aattatcaaa aatactattg
     6421 gcattactgt atttttgaac taaagtttga agattatttt tcattgtttc atcttcggca
     6481 gagaatccgg cattccatgc ctgatatttt gcattatcta gcacaacctc gccatttcca
     6541 cctagattat ctaagctttt taacatattg tctattgggg tcatgtttat actgacaaca
     6601 tatcccccgt ttttttgaga taccttgccg attgttccac ctaattctgt aagccattta
     6661 tttgcttgtt cctgactaac agtattattt gctggatata gcggtttatc tttatatttt
     6721 tccttgagtt cttccaatgc ctttttaagc gagttgactt gtaatttcac ggagtttccg
     6781 tcgttacctc cgggagagat ccagccggca agactggaaa gaacagcgct aaaatcttga
     6841 tacatttgag tatatgaact aacggcatgt tcatatactt tcagatactg ttcattaata
     6901 tcattgatgg agtttgcaat tttagcccac agttctctat gagatatcat ttgatctcca
     6961 tcaagctcgg cttctttcgg agctgaatgt aataattctc ttgcgtcttt attaattgga
     7021 tattcgtgcc tggaaagaat atctaatagt tgagcggatt tatttacatc catgctaatc
     7081 tgagatgaat gtaatgctat ttcttctagc gatgttttag tcaacgtttt ttgtgaaagc
     7141 tctttcttta atgcctgatt tgttgttctg atattatgaa gggtgtcgtt gagcatagta
     7201 agggaactta caggatgaga actggtcgtt gtttttatat cagaattaac tgtttcggtt
     7261 gatgaaccgt tggtattgtt tggactgaat gatgaggtgg aaatactatt agtcagagtt
     7321 gttatattca tggttatttc cttacaggaa gagccatata ttattatggc tcttggatat
     7381 attaagctcg aatgttacca gcaatctgac tggctgtcga attctttgat tggttgatgc
     7441 tgtcaattat attcaataat ttttgtatta attgatttgt cgcttgggat gcttctttag
     7501 atacttggga tgcttcctct gcttgtttac tgctggcctg actgattagt tgttcttctt
     7561 cttcaagagc agatgcataa cgccctcctg atgtggatat attaccagca acggctgatg
     7621 tttccattgt tgcacggcca atgttttgtt tttgctgcgc agaaagggta ttgagctcgt
     7681 aagttcttct ctgggtgtca attttatcct gcaaagaaat atcgggagaa ctaagagaag
     7741 ttttatgttc agttgacagg gatatattat ctggcgccag tttattttta cctaaaaatt
     7801 ttgtgctaga gttagtttgt ggtgaggtga tatttgtatc tatttgttta tttaacccta
     7861 atttagaacc tgcaagctct ttttcaagag attgagcagt ggcaaggttc tttcttaagg
     7921 ctcctttttg gtcgctaatc cctgaatgcg tttttttggc accgataccc gttataccta
     7981 cttgtgtgac tgctccagta atgcttgatg atagggctgc caggccttgc cgaacaatgt
     8041 tctctgcagc tgattttgta gcatcgaacg caatcaatga caattgagag cccaatttag
     8101 tttctgcagt gcggagagca gaaagtagaa cggctacact aataattaaa gaaacagcat
     8161 tagaagaaag actggaaata tccagagtgt tctctggagt aagggtgtga ctcatctctg
     8221 aaagatcttt catctttttg tcagtaaggc gagcaatgat ttctgaatca tgctgaacct
     8281 gctctgataa ctttaaaagt tgatcatcat ttaatgtggt tgttaatacg ggatttttac
     8341 cgacattaag tggaatatgc gctgcaatct gctgataatt ttgtgatttt tgtgtttcgg
     8401 aagaactttg agtttgtttt gtggatatat ctgtatataa aatctgggtt ggttttgtgt
     8461 tttgaatttc cataacattc tccttatttg tatcaagcag tagtttgttg caaaattgct
     8521 tttgcaacat cagttctatt agcctgagag ttggacattg aggctaatag atctgcaatt
     8581 acttcctgca attggccgaa tttttctatt gcttcactga tatattttga cagttgttca
     8641 acttgatatt tcgataatgt caggtctgct agatttgtcg acgcgctgtt ctggaaaaca
     8701 gcagaagcga cacttcctcc cgcttgtgtg gcagagttaa cactttctcc taataaaact
     8761 gcttggttta aatgggtgga aataatttgt ttggatatta cttcatcacc cgctgcacca
     8821 agaaatttat ttaatctggc aacagcatta gtgattaaat cgtccagttg agaagaaaaa
     8881 ttcttgagaa actttggtat aaggtctgtg agggttttac ctattatttt gccaatattt
     8941 tctgcaagtt ttgctgctgc ctgtttacca acagtggcta cgagaacgac tgctgcaact
     9001 aggacaagag cgcctgcgat tgcccccaga atagagccaa tcattttggc ttttttcgag
     9061 tcgacgccca agccttcgag catttttgta aatgcatctg aaaggagttt gattaagggt
     9121 tcaatgactg ctttcatgat cggattcagg gcttgttcca tgaaggaatt gccggtcgct
     9181 gcttgtacta tagcatccgt aaccataaga gctaaaccaa cagctgccag tgctagagag
     9241 gctcctccag aaaaagctgc tgcaacaaca ctaacgatag ttaaaagtgc cccaagtatt
     9301 ttcccaacac aacccattac tctgttgagt tcttctgctt tacgtacttc agcagcatac
     9361 tcatcagatt ttctctccat ttcagttttt cttgattctt ggagagactg gaatagagcc
     9421 agatcatttt ttaaagattc ttcattattt tttccaacta gttgaataaa ggttgccatc
     9481 aattgagtaa cactggcaag tccggttaat gatttctgct gggttgatag ctgttcagca
     9541 gatgctgtgt ttgaaaatgc agaaaaagag tctatttctt tttcgagttg cattgattta
     9601 tctgtaagtt tgctatgaat tgacagggtt ttctgctcaa tcaatgtcct gtctttaact
     9661 gctgcgtctt ttttgatagt gagttgtatt tcttcccggc ttaatttttt cttttctggt
     9721 gactctgggt cgagttcgga taatcttgtt tgaatttggt taattttatt ttctaggtct
     9781 tttattttag aatctgcgtt ttttagttta ttaatttgtt tttcatagtc tctggttagt
     9841 ccttcagttt cagatagaag agtgttaatt ttatcggaga attctaggtt tttttgctgt
     9901 cttgcctgtt gctgggactt ccaagcagta attttatttg ttaatgcagt taaagatttt
     9961 tcaccgagta tttgaataag gtttccaatt aaaagcgtta gttgggaact tgcatttaat
    10021 gactttggtg ctttaagttc agggattaat atatttgaag ttgagtgtgc attagttgta
    10081 ttaatatttt ggttagcagt aagatcagca attgtaagag aaaataattt gttagctgca
    10141 tcatttgcag cttggatagt attgtctcca agctcagtgg aagccaatat tttggcaaga
    10201 ggaaaaccag tggttgtggt gcttacatta tgcataataa ttactccttg atatcctgaa
    10261 ttgcgtccaa gtatgattgt gcttttattt ttaatttttc atcattgctg tgttgaatta
    10321 cgagttcgaa gcactcttta gcttttaagg gggctttcaa ccgaagctgg cattgtccag
    10381 tatggaatac tggtgtatag tcattttttc ctaatgcaaa agcgacagca taaaggtctg
    10441 ctgcttgttg gaactgttct tttatctgat aaatagctgc gagtcccata atgtagtcta
    10501 cattgtaaaa gtcgtatata cataaaaacc tgaagaaaac ttcagcttcc tctattcttc
    10561 ctttgttgta aaagtcataa gcatatgaat aaatgtcatc catcatatca tcaggaattg
    10621 cattaatatc tttcagtgta gcgccagagt taattgcatc aattactgca gtagagatgc
    10681 tttcattttc ggtgatattt aaagacataa ggtctccttt ttgtctgctt tactttaata
    10741 agtataagat ttaatataaa agatttaatt tgtattgctt tgacggtata cagcctttag
    10801 aacctctgcg cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta
    10861 gacggaactg gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc
    10921 actgctatag cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc
    10981 tgtatgactg atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt
    11041 ttcaatagct gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat
    11101 ggccatttca tctatacttc tgtttatcat tgaagagaca taagtggttc gctcttggct
    11161 catccaacaa aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc
    11221 cctgatttta tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa
    11281 gttaaaaaca gataatattt tctttacaag cttattgcga cttaacgaat caaatgatgg
    11341 gcgaggaatc gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc
    11401 tatatgttag ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa
    11461 ttcatacaaa gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt
    11521 caaaacacat tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa
    11581 agagtagtta aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg
    11641 ttcattaaga agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat
    11701 ttgttcttcg agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt
    11761 tatttcataa agtttatcat atagtttgcg acacatttct atggccttct atatattaga
    11821 atgagagtta ttcaataaaa gcttattgat ctgtaataag tcgtgaatgt aaagtttggt
    11881 tccaagatct ggcgatttaa gagaattgta ataatcaata atttttattg atttttcgat
    11941 gatttcttct tttttatctg ttgcttcggt ttttaattcg tttaatacac gagtcatatt
    12001 gctgggtgct gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc
    12061 tgaagataca ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt
    12121 tggtgtgtgg ataaggttaa ttttattgaa acttttaaga tagctgcgtc gtaattgagt
    12181 caatctggaa agatcaaaat cagattcaat atacttcatt atttcgttat aagactgatt
    12241 gagactttca atgcgttgcc taactttatt tgcatacgcg tatacatcgt taggtgtagc
    12301 aaccaattta acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt
    12361 aagtgccata ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct
    12421 gaaagcatct gctttctttt tgcatataaa ttttgacatg tttgtttcat caaggccaaa
    12481 aaggttatgg ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac
    12541 tttatcagca ctgacccccc aataaccata ggggtctttt ttttgattag ctctgggaaa
    12601 aaatattttt ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg
    12661 gcctgcatca agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgcttata
    12721 ggtatcgaca ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt
    12781 aacattcccg ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt
    12841 ttttatactg atactagtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa
    12901 aactaaatgt ctttcaagat ttttggctgt accgatgcca tgaaaacgct ctgcggtatt
    12961 caacattaat ttgatgtctc tacctgtgaa agagtgttga attgagtttt tagctgcttc
    13021 ataggcggca aacgcacttt ctgcagattt ttcgctagct aatgtattga ggaaaagcaa
    13081 taaggatttt tgattctcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt
    13141 tatctttgca ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg
    13201 tgctatcaat ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct
    13261 tgcgtcaatg aaattgctaa ttttgaggat catactttat taactctcca ttacttggtg
    13321 attgtgggga tgtaaaatgt atttggtata taatatattc aattaataaa tttagaaact
    13381 tgagcctgtt aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat
    13441 atgtagtgct cgtttcatca tgaaatccca caagataaag tgcctgatgt atcaggctcg
    13501 gagtgttata gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag
    13561 gtacaaaact gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa
    13621 tttgggattg catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac
    13681 cggaaagcac aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa
    13741 taaggggatc gaagcgctta atcgattata tttacagaac caaacaagct taacaggaaa
    13801 aagccttttg tttgcgcgtg acagagctgg agttttttgc gaggcaataa agttggcagg
    13861 tggtgatacg tcaaaaatta aagccatgat ggaacgatta gatacctata aacttggtga
    13921 agttaataaa agacatatta atgagcttaa taaagtaata agtgaagaga taagagcaca
    13981 gctaggcatt aaaaataaaa aggaattgca aacgaaaata aaacaaatat ttactgatta
    14041 tttaaataat aagaattggg gaccggtcga caagaatatt agtcatcatg ggaaaaatta
    14101 tggttttcaa ttaactcctg cctctcatat gaagataggg aataaaaata tatttgtcaa
    14161 agagtataat ggaaaaggaa tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa
    14221 tatgtggctt tcaaaagtgg tggatgatga ggggaaggaa attttttcgg gtatcagaca
    14281 tggagttata tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg
    14341 taataaagct gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca
    14401 ggctttgtct ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac
    14461 cagtttaacc gggggagagg agagtatgtt aaaagatcag gtgaacgcat taaaagggtt
    14521 aaactctaaa agggggaagc caacgaaatt attaattcgg aatagtgatg gtcttctgaa
    14581 agaagtaagc gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct
    14641 aaaaatgggc ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt
    14701 gttgggggat aatttcctca aaaatggtgt gattggaggt tgggctgcag aagcaataga
    14761 aaaaaatcca ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt
    14821 aaccaaaaag ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagaatgac
    14881 tctattggct tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag
    14941 aacaggtatg caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca
    15001 gttttctcaa ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct
    15061 aatgaatagt ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt
    15121 catgaaaaaa ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc
    15181 aaaaatatgg aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga
    15241 tttagcagat gttatttgcc gagccttggg tatcccttta attgatatcg atgatcaagc
    15301 aatcatgctt gatgatgatg tgctaatata tattgaaaaa gaaggagatt caataaattt
    15361 attatgccct ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct
    15421 aaattactca gaaaagatat gcttagctac tgatgatgaa ggcggaaatc taattgcgcg
    15481 attagatctt accgggataa atgaattcga ggatgtatat gttaatacag aatattatat
    15541 ttcgcgtgtg cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc
    15601 cgtttcgtat ttattttgct gtgttttatc ccatatttag ggagagctga ttgttgggat
    15661 aaggctggtg aaaggtacaa tattccatca agtttgttaa aagcgattgc ggaaaaagaa
    15721 tccggattta ataaatctgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata
    15781 atgcaaataa atgactttca ttctaaaaga cttagagaaa tgggatattc tgaggaaatg
    15841 ctaattagtc atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg
    15901 atgatgtatg gaagagggtg ggaggctgtt ggagcctata atgcaggaac gtctccgaaa
    15961 aagaaaaaag aaagattaaa gtacgcagag gatatctata gacgctattt gcggatagca
    16021 gcagagagca agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact
    16081 caaatcttgc accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc
    16141 cgttatatta tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg
    16201 gaaacgacaa tttcccagaa aatattattc ctgtaacaga tagtaaatcg gatggtataa
    16261 tttatttgac cataagcaaa gataatatct gtcaatttag tgatgaaaaa ggtgagcaaa
    16321 ttgatatcaa ttcacaattt aattcatttg aatatgacgg gatttctttt caccttaaaa
    16381 atatgaggga ggataaatcg agaggccata tactaaacgg aatgtataag aaccactcgg
    16441 tgtttttttt ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga
    16501 aagatgaagt aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca
    16561 ctggacagtg caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcgtctgtag
    16621 ccttaaataa aaccggattt accaaatgca gatatattct ggtgtcaaac aaagaaataa
    16681 atagaattca acaatatatc aatcaacgat tcccatttat caatttgtat gtgttgaatt
    16741 tagtgagtga taaagcggaa ttactggtgt ttttaagtaa agaaagaaat agttcaaaag
    16801 ataccgaact tgataaattg aaaaatgctc tgattgttga atttccgtat atcaaaaata
    16861 ttaaattcaa ctatcttagc gatcataatg caagggggga tgctaaaggt attttcacaa
    16921 aggtaaatgt ccagtataaa gagatttgtg aaaataataa agttacatat tctgtcagag
    16981 aagaactgac agatgaaaag cttgagctca ttaatagact gatttctgaa cataaaaata
    17041 tatatggaga tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta
    17101 aatcatatct taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt
    17161 tagataaaaa taagtgagga taaaatgagt gttacagtac cgaatgatga ttggacattg
    17221 agttcattat ctgaaacttt tgatgatgga actcaaacat tacaaggtga actaacattg
    17281 gcactagata aattagctaa aaatccttcg aatccacagt tgctggctga ataccaaagt
    17341 aaattatctg aatatacatt atataggaac gcgcaatcca atacagtgaa agtgattaag
    17401 gatgttgatg ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt
    17461 tatccagtca atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca
    17521 agtctggaag acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa
    17581 gtatcacaaa taatggagat ggtaagcaat ccagaatcat taaacccaga atctttggcc
    17641 aagttacaga cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca
    17701 agaaaaacag tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata
    17761 aaggttttat tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa
    17821 tttctaattt atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata
    17881 ttactgctag aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga
    17941 catttgcatc ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg
    18001 atatctcaca aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc
    18061 gtttatatag tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta
    18121 tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga
    18181 tgcatatatc agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta
    18241 atattaagcg atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca
    18301 tattaactcc gaaagaagaa tatgtttata caaatgtaca acctgttaag gaagttaaat
    18361 cggaattttt aacaaatgaa gtaatatatt tatttctcgg gatggctgta ctagttgtca
    18421 ttcttttggt atgggcattc aaaacagggt ggttcaagag aaacaaaata tgataagaat
    18481 ggatggaatt tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag
    18541 aaatcggttg aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa
    18601 taatctcata attaataaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac
    18661 cgctatgttt gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca
    18721 agagtctcgg ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag
    18781 taagcaaggg gaggccgcta ttcgaagtgg tttttggcat cttattgcgt ggaaggaaaa
    18841 tatatctgtt ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg
    18901 aaattatacg cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa
    18961 aaggcacttt accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg
    19021 aaaaggaatt atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt
    19081 gtgttataaa ggaggctacg aaaaaagctg aatcactgcg tatagatgct gtatgtgatg
    19141 gttatcaaat tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga
    19201 agttaaagca aaatgaaaat agaagaaata ttgaggacta tataactagt ttgctttcag
    19261 aaaatttaca tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc
    19321 gtaatacagt aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa
    19381 agttagaact tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg
    19441 aagggaataa ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg
    19501 taatatcagg tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt
    19561 tcaaagagtt agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc
    19621 ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa
    19681 gaagttgtta atttaaatga gagattatca tcttcgcaat catttgatga ggatataatc
    19741 tatgaaataa tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg
    19801 cacaataaga ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa
    19861 agttcacttc ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt
    19921 tatgcatcat tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat
    19981 tcattaatta gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa
    20041 gttgaaaata tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc
    20101 atcatctaat tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat
    20161 tccaaatgat ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa
    20221 atgtattagt ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa
    20281 tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt
    20341 ttctatgctg aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat
    20401 agagaaggat ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta
    20461 aatataaagg tctaaataca agtaatatgt tttacattta ctctagtgga catgaaccag
    20521 ttaacgttga gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga
    20581 agggtttttt tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta
    20641 tcgctttaac attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc
    20701 tctagtagag catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg
    20761 ggatagttgt aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc
    20821 tgaccagaat gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt
    20881 tctttatctt ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga
    20941 gcattatggt gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa
    21001 agtcaaagaa caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca
    21061 caatcaaaca atcacttcag ctgccatgaa taatgggtat gcatcaacat cccatttttc
    21121 aaatgagata aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt
    21181 agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt
    21241 taccattgat agttaatgct aataatattg actctcattt attagaacaa aatgacatag
    21301 cgaaatatgt tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat
    21361 taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg
    21421 atttatctaa tccagaagaa atgcttgaaa aactgacatt acttgtggga ctcatatggt
    21481 ataaagatgg aaatgcatta tacatatatg actctggaga attaataagc aaagttattt
    21541 tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg
    21601 atcacagata tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc
    21661 cggcattggt tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta
    21721 ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca
    21781 gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac
    21841 gtttattaaa taatggtaag gctctgagta acagacaagc ccaaaatgat ccgatgccac
    21901 cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta
    21961 ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat
    22021 tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact cagttagata
    22081 tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa
    22141 ataaccttgg tgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcgtcat
    22201 ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag
    22261 ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac
    22321 aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg
    22381 agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct
    22441 tttcatctcg gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc
    22501 agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca
    22561 aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc
    22621 atgaaacgaa ctctaacgaa ataatatcta ttccttttct ctcctctatt cctgtaatcg
    22681 gtaatgtgtt caagtataaa acaagtaata tcagtaatat tgtaagggtt tttctgattc
    22741 aaccccgtga gataaaggag tcaagttatt ataatactgc agaatataaa agcctcatat
    22801 ctgaaagaga aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag
    22861 acgaaaaatc attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg
    22921 atgttaaaaa tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa
    22981 attcagatga tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca
    23041 agtatattga ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag
    23101 accttgagaa actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag
    23161 aagatgaaat taatcacaag atttttgatt taaagagaac gttaaaagat aaccttcctc
    23221 tagatcggga ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct
    23281 tagcattaag ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaattattaa
    23341 ctaaaattat taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt
    23401 cagctataca ggctaaatta tttggcaaca aaatgaaact tgaaccacag cttttgcgtg
    23461 catgttatcg tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc
    23521 ttggtaattt tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga
    23581 ttgtagacat ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat
    23641 ctaaattaat tgcaattaag atgattagaa cttcagacgt aatttttatg aagaaactgg
    23701 aatcctcaag cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat
    23761 tatatatttt tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagaagtat
    23821 cacgagccag tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa
    23881 gtcctcatga tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag
    23941 agcttgtcac aagtgcatac aagaaagagc tttctagata acaggagata aaagtgatcc
    24001 agtcttttct taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta
    24061 tgatcattgc tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc
    24121 ttaatattgt acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga
    24181 gtttttctac attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa
    24241 taagtactag tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg
    24301 gccagtttgt cattggtgat agtctggctg taggttttgt tattttttca atagtaactg
    24361 ttgtgcagtt cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct
    24421 tttctcttga cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa
    24481 ttattgatgc cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt
    24541 atggttcatt tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta
    24601 ttatttttgt caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtccc
    24661 tatctggtgc attatctact tataccatac taactattgg tgacggattg gtctctcaga
    24721 ttcctgctct gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata
    24781 gcgataatat gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg
    24841 ttacatcagc tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt
    24901 tcctgatagc agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa
    24961 aaagtctgtc tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc
    25021 atgactcatc tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt
    25081 taattttatt atttgccgaa aataagataa atgcaaatga tatggaaggt cttattgaaa
    25141 ggataagaag tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa
    25201 caagcaatga gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata
    25261 gttttaatat atattttgac aaagtgtgta ttacagatga aaatggagat atagatgctc
    25321 taggtattcc tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt
    25381 catatacaga aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt
    25441 atcaccagtt gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa
    25501 caaaaaatat gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc
    25561 gacatgtgac tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt
    25621 ctgttcgcaa tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag
    25681 atgtcataac attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa
    25741 tagctgtttc tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa
    25801 gaaaagggat aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg
    25861 atgaggtaat ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt
    25921 tcgttctttt ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat
    25981 ttaagagtat actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat
    26041 taaagactat ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag
    26101 atagtctttt cacgattggt tgtcctccat caattattac agatttagac agccattcag
    26161 cgattaccat ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg
    26221 ttatgttatg ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca tctgcttata
    26281 acatattaaa tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc
    26341 atcgttctga tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg
    26401 ggattgtttt tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgggg
    26461 tcttataatc aatgagctat acaaaattgc tcactcaatt atcttttcct aatagaatct
    26521 cggggccaat cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc
    26581 aggctggaat tgaaagtaat gaaattgttg caagagctca ggttgtagga tttcatgatg
    26641 aaaaaacaat attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta
    26701 agcccactgc ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca
    26761 atcctttagg ggaggttact gataagtttg ctgttacaga taacagtgaa attctttatc
    26821 gacctgtaga taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt
    26881 taacaggtat taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga
    26941 tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg
    27001 gtgctgatat atatgttatt gggttaattg gtgagcgagg tcgagaggtt actgaaacgg
    27061 ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg
    27121 attactcttc ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt
    27181 ttaggactga aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag
    27241 cattacgtga tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt
    27301 cggtttttga tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct
    27361 ctattactgc attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg
    27421 aagaggtaag atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag
    27481 gacaatttcc tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg
    27541 atgaaaaaca tcgtattatg gccgctgcat ttcgggagct actttctgaa atagaagagc
    27601 taaggacaat tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa
    27661 tctacaacaa aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta
    27721 cttatgagca gacaatggag cttattggtg aaacaattag ataaggtttt aaaaatcaaa
    27781 gataaatatc aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa
    27841 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
    27901 gaatgactta gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
    27961 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
    28021 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
    28081 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
    28141 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
    28201 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
    28261 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
    28321 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
    28381 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
    28441 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa
    28501 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
    28561 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
    28621 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
    28681 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
    28741 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
    28801 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
    28861 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
    28921 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca
    28981 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
    29041 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
    29101 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
    29161 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
    29221 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
    29281 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
    29341 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
    29401 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
    29461 taatcaagtt tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa
    29521 tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact
    29581 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
    29641 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt
    29701 ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa
    29761 tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
    29821 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
    29881 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
    29941 tgctgagtga catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
    30001 ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
    30061 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
    30121 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa
    30181 aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc
    30241 aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag
    30301 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
    30361 catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat
    30421 ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
    30481 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
    30541 gtatcctgtc taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg
    30601 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
    30661 cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt
    30721 gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
    30781 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
    30841 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
    30901 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
    30961 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
    31021 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
    31081 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
    31141 ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt
    31201 agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt
    31261 ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa
    31321 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
    31381 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
    31441 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
    31501 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
    31561 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
    31621 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
    31681 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt
    31741 attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta
    31801 tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg
    31861 gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg
    31921 ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt
    31981 tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa
    32041 tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt
    32101 aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt
    32161 tgggggaggc tgtttaagga tataatatta tttttcttgg cattttctat tcttgttatt
    32221 attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag
    32281 gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt
    32341 gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta
    32401 gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct
    32461 gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca
    32521 aatgaagttg gtataccgac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca
    32581 catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt
    32641 tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt
    32701 aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt
    32761 aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata
    32821 aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt
    32881 tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact
    32941 cttttgctga tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag
    33001 aaaattgtta tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac
    33061 gactgaccat aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg
    33121 ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac
    33181 taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta
    33241 aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac
    33301 caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt
    33361 tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg
    33421 gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag
    33481 gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct
    33541 cttaaaacga tagtggctca ggctttcatt tttcagcgtt ccccagaag
//
LOCUS       DAJTUM010000037        33512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTUM010000037 DAJTUM010000000
VERSION     DAJTUM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            76..1353
                     /gene="waaA"
                     /locus_tag="OO471_001891"
     CDS             76..1353
                     /gene="waaA"
                     /locus_tag="OO471_001891"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6243180.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1361..1840
                     /gene="coaD"
                     /locus_tag="OO471_001892"
     CDS             1361..1840
                     /gene="coaD"
                     /locus_tag="OO471_001892"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6243181.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1879..2688)
                     /gene="mutM"
                     /locus_tag="OO471_001893"
     CDS             complement(1879..2688)
                     /gene="mutM"
                     /locus_tag="OO471_001893"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6243182.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2786..2953)
                     /gene="rpmG"
                     /locus_tag="OO471_001894"
     CDS             complement(2786..2953)
                     /gene="rpmG"
                     /locus_tag="OO471_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6243183.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2974..3210)
                     /gene="rpmB"
                     /locus_tag="OO471_001895"
     CDS             complement(2974..3210)
                     /gene="rpmB"
                     /locus_tag="OO471_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6243184.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3427..4095)
                     /gene="radC"
                     /locus_tag="OO471_001896"
     CDS             complement(3427..4095)
                     /gene="radC"
                     /locus_tag="OO471_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6243185.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4267..5487
                     /gene="coaBC"
                     /locus_tag="OO471_001897"
     CDS             4267..5487
                     /gene="coaBC"
                     /locus_tag="OO471_001897"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6243186.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5465..5923
                     /gene="dut"
                     /locus_tag="OO471_001898"
     CDS             5465..5923
                     /gene="dut"
                     /locus_tag="OO471_001898"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6243187.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6030..6626
                     /gene="slmA"
                     /locus_tag="OO471_001899"
     CDS             6030..6626
                     /gene="slmA"
                     /locus_tag="OO471_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6243188.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6663..7304)
                     /gene="pyrE"
                     /locus_tag="OO471_001900"
     CDS             complement(6663..7304)
                     /gene="pyrE"
                     /locus_tag="OO471_001900"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6243189.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7370..8086)
                     /gene="rph"
                     /locus_tag="OO471_001901"
     CDS             complement(7370..8086)
                     /gene="rph"
                     /locus_tag="OO471_001901"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6243190.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8213..9076
                     /locus_tag="OO471_001902"
     CDS             8213..9076
                     /locus_tag="OO471_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6243191.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9297..10121
                     /gene="dinD"
                     /locus_tag="OO471_001903"
     CDS             9297..10121
                     /gene="dinD"
                     /locus_tag="OO471_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6243192.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10412..11029
                     /locus_tag="OO471_001904"
     CDS             10412..11029
                     /locus_tag="OO471_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6243193.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11026..12708)
                     /gene="ligB"
                     /locus_tag="OO471_001905"
     CDS             complement(11026..12708)
                     /gene="ligB"
                     /locus_tag="OO471_001905"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6243194.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12966..13589
                     /gene="gmk"
                     /locus_tag="OO471_001906"
     CDS             12966..13589
                     /gene="gmk"
                     /locus_tag="OO471_001906"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6243195.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13644..13919
                     /gene="rpoZ"
                     /locus_tag="OO471_001907"
     CDS             13644..13919
                     /gene="rpoZ"
                     /locus_tag="OO471_001907"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6243196.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13938..16046
                     /gene="spoT"
                     /locus_tag="OO471_001908"
     CDS             13938..16046
                     /gene="spoT"
                     /locus_tag="OO471_001908"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6243197.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16053..16742
                     /gene="trmH"
                     /locus_tag="OO471_001909"
     CDS             16053..16742
                     /gene="trmH"
                     /locus_tag="OO471_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6243198.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16748..18829
                     /gene="recG"
                     /locus_tag="OO471_001910"
     CDS             16748..18829
                     /gene="recG"
                     /locus_tag="OO471_001910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6243199.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18863..20068)
                     /gene="gltS"
                     /locus_tag="OO471_001911"
     CDS             complement(18863..20068)
                     /gene="gltS"
                     /locus_tag="OO471_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6243200.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20348..21739
                     /gene="xanP"
                     /locus_tag="OO471_001912"
     CDS             20348..21739
                     /gene="xanP"
                     /locus_tag="OO471_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6243201.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21860..23569
                     /locus_tag="OO471_001913"
     CDS             21860..23569
                     /locus_tag="OO471_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6243202.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23622..25940)
                     /gene="yicI"
                     /locus_tag="OO471_001914"
     CDS             complement(23622..25940)
                     /gene="yicI"
                     /locus_tag="OO471_001914"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6243203.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25950..27333)
                     /locus_tag="OO471_001915"
                     /pseudo
     CDS             complement(25950..27333)
                     /locus_tag="OO471_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27626..27720
                     /locus_tag="OO471_001916"
     tRNA            27626..27720
                     /locus_tag="OO471_001916"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27660..27662,aa:Sec,seq:tca)
     gene            27992..29203
                     /locus_tag="OO471_001917"
     CDS             27992..29203
                     /locus_tag="OO471_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6243204.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29615..30658
                     /locus_tag="OO471_001918"
     CDS             29615..30658
                     /locus_tag="OO471_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6243205.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31173..31601
                     /locus_tag="OO471_001919"
                     /pseudo
     CDS             31173..31601
                     /locus_tag="OO471_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31631..32782)
                     /locus_tag="OO471_001920"
                     /pseudo
     CDS             complement(31631..32782)
                     /locus_tag="OO471_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32847..33495
                     /locus_tag="OO471_001921"
                     /pseudo
     CDS             32847..33495
                     /locus_tag="OO471_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8400 a         8808 c         8256 g         8048 t
ORIGIN      
        1 aaacgggaaa agtaatggta aagccacagc taaatacata gaatccccag cacatccata
       61 agtcagctat ttactatgct cgaattgctt tacaccgccc ttctctacct tattcagccg
      121 ctgatctgga tacggctctg ggtgcgcgga cgtaaggctc cggcctatcg aaaacgctgg
      181 ggtgaacgtt acggttttta ccgccatccg ctaaaaccag gcggcattat gctgcactcc
      241 gtctccgtcg gtgaaactct ggcggcgatc ccgttggtgc gcgcgctgcg tcatcgttat
      301 cctgatttac cgattaccgt tacaaccatg acgccaaccg gttcggagcg cgtacaatcg
      361 gctttcggga aggatgttca gcacgtttat ctgccgtatg acctgcccga tgcgctcaac
      421 cgtttcctga ataaagtcga ccccaaactg gtgttgatta tggaaaccga actatggcct
      481 aacctgattg cggcgctaca taaacgtaaa attccgctgg tgatcgctaa cgcgcgactc
      541 tctgcccgct cggccgcagg ttatgccaaa ctgggtaaat tcgtccgccg cttgctgcgt
      601 cgtattacgc tgattgctgc gcaaaatgaa gaagatggtg cacgttttgt ggcgctgggc
      661 gcaaaaaata accaggtaac cgttaccggt agcctgaaat tcgatatttc tgtaactccg
      721 cagttggctg ctaaagccgt gacgctacgc cgccaatggg caccacaccg cccggtatgg
      781 attgccacca gcactcacga aggtgaagag agcgtggtca ttgccgcaca tcaggcattg
      841 ttacagcaat tcccgaattt attgctcatc ctggtacccc gtcatccaga acgcttcccg
      901 gatgcgatta accttgtccg ccaggctgga ctaagctata tcacacgctc ttcaggggaa
      961 gtcccctcca ccagcacgca ggttgtggtt ggcgatacga tgggcgagtt gatgttactg
     1021 tacggcattg ccgatctcgc ctttgttggc ggttcactgg ttgaacgtgg tgggcataat
     1081 ccgctggaag ctgccgcaca cgctattccg gtattgatgg ggccgcatac ttttaacttt
     1141 aaagacattt gcgcgcggct ggagcaggca agcgggctta ttaccgttac cgatgccact
     1201 acgcttgcaa aagaggtttc ctctttactc accgacgccg tttaccgtag tttctatggc
     1261 cgtcatgccg ttgaagtact gtatcaaaac cagggcgcgc tacagcgtct gcttcaactg
     1321 ctggaacctt acctgccacc gaaaacgcat tgaggttgtt atgcaaaaac gggcgattta
     1381 tccgggtact ttcgatccca ttaccaatgg tcatatcgat atcgtgacgc gcgccacgca
     1441 gatgttcgat cacgttattc tggcgattgc cgccagcccc agtaaaaaac cgatgtttac
     1501 cctggaagag cgtgtggcac tggcacagca ggcaaccgcg catctgggga acgtggaagt
     1561 ggtcgggttt agtgatttga tggcgaactt cgcccgtaat caacacgcta cggtgctgat
     1621 tcgtggcctg cgtgcggtgg cggattttga atatgaaatg cagctggcgc atatgaatcg
     1681 ccacttaatg ccggaactgg aaagtgtgtt tctgatgccg tcgaaagagt ggtcgtttat
     1741 ctcttcatcg ttggtgaaag aggtggcgcg ccatcagggc gatgtcaccc atttcctgcc
     1801 ggagaatgtc catcaggcgc tgatggcaaa attagcgtag cgtttatgcc ggatggtatg
     1861 ccatccggct cgcatgaatt acttctggca ctgccgacaa taaaacgttg cccgctgcgc
     1921 atgtttagtc gccacaatcg gcgtaccgca cacccgacac ggctcacctt ttcgcccgta
     1981 aacctgcaat tcctgcgcga aatagcccgg tttaccatca ctttgcagaa aatctttcag
     2041 cgttgtacca ccctgctcaa tcgaacgcag caacaccgct ttaatcaccc gagctaacaa
     2101 ttcacactct gccagcgaca gtgatgacgc cagccgatcc ggatggatcc ccgccgcaaa
     2161 cagtgattcg ctggcataga tattccctac ccctaccacc agcttgttat ccatcagcca
     2221 cggtttaatc gccgtttttt tcttcgcgca cttctgatgc agatactcac cattgaaatc
     2281 gtcgctaagc ggctccggtc caagatgggc cagcacatta tgcccttcca gctctttggt
     2341 ccacagccag gcaccaaagc ggcgcggatc ggtgtagcgc agcactttac cgttgctcat
     2401 caccaaatcc acatggtcat gcttttcagg gggaagttct tctggaagga tgcgcaggct
     2461 gccagacatc cccaaatgaa tgataatcca gccctcaggc agctccagca gcagatattt
     2521 agcccgccgc tgcacgctaa gcactggttg atcgcttaat cggtagatct cttctgaaac
     2581 cggccagcgc aagcgtccgt tgcgcaccac cgcatgaaga atggttgcac caacgagatg
     2641 cggttctatg ccgcgacggc tggtttcaac ttcgggtaat tcaggcatag catctccagg
     2701 aatgaacaga tgcaatcaat atgggggcaa gcagataaca aaaaaccccg ccggagcgag
     2761 gtttttcgtt acatcaaagc gagaattatt tgattttcgc ttctttgtag atcacgtgct
     2821 gacgaacaac tggatcgaat tttttcagtt ccagtttttc cggcttagta cgtttgttct
     2881 tcgtagtggt atagaagtga ccagtaccag cagaagaaac cagcttgatt ttctcacgaa
     2941 tacctttagc catgatttat ttcctctaag tacttagtac ttttcgccac gggcacgcag
     3001 ttcagccaga actgtatcga tgcctttttt atcgattaca cgcatacctt tagcagatac
     3061 gcgcagggtg acaaaacgct tctcgctctc aacccagaaa cggtgagagt gcaggttcgg
     3121 caggaaacgg cgtttagtcg cgttcagtgc gtgggaacgg ttgttaccgg tcaccggacg
     3181 cttgccagta acttggcaga ctcgggacat gtctattctc caaaaatcaa attagctcga
     3241 gcttcgtatg gggtattggc gcctcgtcag gctttacagc ccggtcatcg cagttctatg
     3301 tgaactctcg attgccaggc ccaaatgcca aacccgagat tctcaaaggt ggcgtagtat
     3361 acgctgactc agcgatgtgc tcaagtcccg aacagacaaa gatcccgaag gatcgcgcat
     3421 agcgggttaa atccagccgc gttcggcaaa agaaacatac tctccacgcc caatcacgat
     3481 atggtcgagc acgcgtaaat ccatgaactg acaactcttt attatccgtt cagtaatgag
     3541 tttatccgct ttactgggtt cagcacaacc cgaagggtga ttatgtgcaa ggatcagcgc
     3601 cgaggcgttt atttttatcg cttcgcggat aatttcccga ggatggactt caacatggtt
     3661 tagcgtgccg gaaaaaagac ggctatgcgt tataacccgg tgttgtgagt cgagaaagat
     3721 caccataaag atctcccgct cctcacccgt gagctggctt tgtaaaaatt cccgcgtcat
     3781 ctccgggctg agtaaagggc tttcttcacg catccgcacg ttgtagtaac gtcgcgccag
     3841 ttcagcaatc ccctttaact gggcaaattt cgctacgcca attccatgaa caccactaaa
     3901 ttgttcatat tcagaggtta acaagccata aagagagccg aaattctcca gcatctcttt
     3961 tgccagggtt aatacatctt taccgcgcgt tccggtacgc agaaatagcg ccagcagctc
     4021 gacatccgtt aaggcgctaa taccaaactt cagcattttt tcgcgcggca tcaacagctg
     4081 tgcattgttt ttcaccttca cctcctttgt ggtgctcgca tcctgtcata gccgttccgt
     4141 taaatcgacg gccagttttc aatcctggaa agagcctcgc aaagtgaatc tcaggtgaag
     4201 gcacgacaac aaaagtattg tgataaaatc gccaacttct ggtgtcaccc aacaggaaaa
     4261 atcatcatga gcctggccgg taaaaaaatc gttctcggcg ttagcggcgg tattgctgcc
     4321 tataaaaccc ctgaactggt gcgtcgtttg cgcgatcgcg gggccgacgt ccgcgtagcc
     4381 atgaccgaag cggcaaaagc ctttatcacc ccacttagct tgcaggcggt ttctggttat
     4441 cccgtttccg acagtctgct tgacccggca gccgaagccg ctatgggcca tattgagctg
     4501 ggtaaatggg ctgatttagt gattctcgcc cctgccacgg cagatttgat tgcccgtgtt
     4561 gctgccggaa tggcgaatga cctggtatcg acgatttgtc tggctacacc tgcgcctgta
     4621 gccgtgctcc ccgccatgaa ccagcagatg taccgtgccg ctgccacgca gcataattta
     4681 gaggtgcttg cttcccgtgg tttgctcatc tgggggccag acagtggcag tcaggcttgt
     4741 ggtgatatcg gtcctggacg aatgctcgat ccgttaacca ttgtggatat ggcggtggcg
     4801 catttttcgc ccgtcaacga cctgaaacat ctgaacatta tgattaccgc cggtccgacg
     4861 cgtgaaccgc tcgatccggt gcgttatatc tctaatcaca gctccggcaa gatgggtttt
     4921 gctatcgccg ccgctgctgc ccgtcgtggc gcgaacgtca cgctggtatc aggtccagtt
     4981 tcactaccga cgccaccgtt tgttaaacgt gttgatgtga tgaccgcgct ggaaatggaa
     5041 gccgccgtga atgcttctgt acagcagcaa aatattttta tcggttgcgc cgccgtggcg
     5101 gattatcgcg cagctaccgt ggccccagaa aaaatcaaaa agcaggccac gcagggtgat
     5161 gaattaacaa taaaaatggt taaaaacccc gatatcgtcg caggcgttgc cgcactaaaa
     5221 gaccatcgac cctacgtcgt tggatttgcc gccgaaacaa ataatgtgga agaatacgcc
     5281 cggcaaaaac gtatccgtaa aaaccttgat ctgatctgcg cgaacgatgt ttcccagcca
     5341 actcaaggat ttaacagcga caacaacgca ttacaccttt tctggcagga cggagataaa
     5401 gtcttaccgc ttgagcgcaa agagctcctt ggccaattat tactcgacga gatcgtgacc
     5461 cgttatgatg aaaaaaatcg acgttaagat tctggacccg cgcgttagga aggaatttcc
     5521 gctcccgact tatgccacct ctggctctgc cggacttgac ctgcgtgcct gtctcgacga
     5581 cgccgtagaa ctggctccgg gtgacactac gctggttccg accgggctgg cgattcatat
     5641 tgccgatcct tcactggcgg caatgatgct gccgcgctcc ggattgggac ataagcacgg
     5701 tatcgtgctt ggtaacctgg taggattgat cgattccgac tatcagggcc agttgatgat
     5761 ttccgtgtgg aaccgtggtc aggacagctt caccattcaa cctggcgaac gcatcgccca
     5821 gatgattttt gttccggtag tacaggctga atttaatctg gtggaagatt tcgacgccac
     5881 cgaccgcggt gaaggcggct ttggtcactc tggtcgtcag taacacatac gcatccgaat
     5941 aacgtcataa catagccgca aacatttcgt ttgcggtcat agcgtgggtg ccgcctggca
     6001 agtgcttatt ttcaggggta ttttgtaaca tggcagaaaa acaaactgcg aaaaggaacc
     6061 gtcgcgagga aatacttcag tctctggcgc tgatgctgga atccagcgat ggaagccaac
     6121 gtatcacgac ggcaaaactg gccgcctctg tcggcgtttc cgaagcggca ctgtatcgcc
     6181 acttccccag taagacccgc atgttcgata gcctgattga gtttatcgaa gatagcctga
     6241 ttactcgcat caacctgatt ctgaaagatg agaaagacac cacagcgcgc ctgcgtctga
     6301 ttgtgttgct gcttctcggt tttggtgagc gtaatcctgg cctgacccgc atcctcactg
     6361 gtcatgcgct aatgtttgaa caggatcgcc tgcaagggcg catcaaccag ctgttcgagc
     6421 gtattgaagc gcagctgcgc caggtattgc gtgaaaagag aatgcgtgag ggtgaaggtt
     6481 acaccaccga tgaaaccctg ctggcaagcc agatcctggc cttctgtgaa ggtatgctgt
     6541 cacgttttgt ccgcagcgaa tttaaatacc gcccgacgga tgattttgac gcccgctggc
     6601 cgctaattgc cgcgcagttg cagtaatatg acgccggatg acttttcatc cggcgagttt
     6661 ctttaaacgc caaactcttc gcgataggcc ttaaccgccg ccagatgttc cgccatttcc
     6721 ggcttctctt ccagataggc aatcagatct ttcagggtga tgatagagat cactttgcag
     6781 ttgtaatcac gctcaacttc ctgaatcgcc gaaatctcgc cgcgcccgcg ttcctgacga
     6841 tcgagcgaaa tcaacacgcc agcaagcgtc gcgccattgg cctgaataat ctccatcgac
     6901 tcgcgaatcg ccgttccggc ggtgatcaca tcatctacca gcattacgcg tccttgtaac
     6961 gcgctaccaa ccagattgcc gccttcaccg tggtcttttg cttctttgcg gttaaagcag
     7021 tacggcaggt ccaggtcgtg atgctccgcc agtgccacag cggttgtggt ggcaatcggg
     7081 atccctttgt aagcagggcc aaacagcaga tcgaactcaa tgccggaatc caccaacgct
     7141 tcagcgtaaa aacggcctaa cagtgccaga tcgcgcccgg tattaaacag cccggcgttg
     7201 aagaaatagg ggcttttgcg cccggatttc agcgtaaact cgccaaactt taacacctgc
     7261 ttgctaagcg caaattcaat aaactggcgc tgatatgatt tcatgccttc gctcctcatc
     7321 ttacttttct acagacaaaa aaaaggcgac tcatcagtcg ccttaaaaat cagtttgcca
     7381 gcgccgcctt ctgcgtcgct acaatggatt cgattccccc tcgggccagc gccaacaagg
     7441 tgagtagctc ttcatgggtg aacggctcgc cttctgccgt cccctgcact tcaatgatgc
     7501 gcccgtcttc ggtcatcact acgttcatgt cggtctctgc ggctgagtct tcaacgtatt
     7561 ccagatcgca aattgcttca ccgtttacaa taccgacaga aactgcggct accatccctt
     7621 tcatcgggtt ggttttcagc ttgccgtttt ccaccagctt ctgtagcgca tctgccagcg
     7681 ccacgcaggc acccgtaatc gacgcggtac gagtgccacc atcagcctga agcacgtcgc
     7741 agtccagcgt aatggtgaac tcacccagcg ctttcaaatc tactgccgcg cgaagagcac
     7801 gggcgatcag acgctggatt tccattgtgc gtccaccctg cttacctttc gccgcttcac
     7861 gagcgttacg ggtgtgggta gaacgtggca gcatgccgta ctctgcggtg atccagccct
     7921 ggccctgacc tttcaggaag cgcggcacgc cttcttcaat agaggcggta cacaatactt
     7981 tggtatcgcc aaattcgacc agcaccgagc cttctgcatg ttttgtatag ttacgagtca
     8041 gggtaacggg acgcacctga ttattgctac ggcctgctgg acgcatattg aaatctccgg
     8101 cttgaaacaa atgtggctgc gcattatacg gacttccggc ggttattcct atcctgacaa
     8161 ggcatcgatg gctataatcc ttccacctct ccttttataa acaggaacgt ctatgatccg
     8221 cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa tgggggagcg caacctggga
     8281 aatgcgctcg gtaaaccagc gttatctgga aacttacttt cgtctgccgg agcagttccg
     8341 tagccttgaa cctgtcgttc gcgagcgtat tcgttctcgc ctgacgcgcg gtaaagtgga
     8401 atgtaccctg cgctatgagc cagatgttag cgcacaaggt gagctgatcc tcaatgaaaa
     8461 actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa atgaagagtg acgaagggga
     8521 aatcaacccg gttgatattc tacgctggcc gggcgtgatg gcagcccagg agcaggatct
     8581 cgacgcgatt gccgctgaaa ttctcgcggc gttggatggt acgctggacg actttatcgt
     8641 cgcgcgcgaa accgaaggtc aggcactgaa agcattgatc gagcagcgtc tggaaggcgt
     8701 caccgccgaa gtggtcaaag tccgcgccca tatgccggaa attctgcaat ggcagcgtga
     8761 gcgtctggtc gcgaagctgg aagatgctca ggttcagttg gaaaacaacc gtctggagca
     8821 ggagttggtt ctgctggcac aacgaattga cgttgccgaa gagctggacc gcctcgaagc
     8881 gcatgttaaa gagacgtaca acattctgaa gaaaaaagaa gcggttggtc gtcgtctgga
     8941 ctttatgatg caggagttca accgtgagtc gaacactctt gcgtcgaaat ctatcaatgc
     9001 cgaagtgaca aactccgcca tcgagctgaa agtgttgatt gagcagatgc gcgagcagat
     9061 tcagaatatt gagtaactcg taacaccaat tctgactttt cagccttcac aaatgccgcc
     9121 cactcaaaca gagcggcatt tttcttcccc gcaacttcaa ttctgttttg cgtgcctgct
     9181 ccagattttg cgatgttttt ttgcccagca cactgagaac gtgagatact cacaactgta
     9241 tataaataca gttacagatt tactttcttt gcaatcgata tcacatggag tgggcaatga
     9301 acgaacatca tcaacctttt gaagagataa aactgattaa tgcaaacgga gcagaacaat
     9361 ggtcagcaag acaacttggg aaactactgg gttattcaga gtatcgtcac tttatacctg
     9421 tattaacgcg cgccaaagag gcctgtgaga acagtggtca cacaattgat gaccatttcg
     9481 aggagatcct cgatatggtc aaaattggct caaatgccaa aagagcatta aaagacatcg
     9541 tactctcccg ctatgcctgt tacctggtag tacaaaacgg cgaccccgcg aaaccggtca
     9601 ttgcggcagg gcagacttat tttactatcc agacccgacg gcaggagctt gctgacgatg
     9661 aagcatttaa gcaacttcgt gaagacgaaa agcgtctgtt tctgagaaat gagttgaaag
     9721 aacataacaa acaattggtt gaggcagcac agcaagctgg ggtagcgaca gctactgatt
     9781 tcgccatatt tcagaatcat ggttaccagg ggctgtatgg tggattagat cagaaagcca
     9841 tccaccaact gaaggggctg aaaaagagtc agaagatcct ggatcatatg ggttcaacag
     9901 aactggcggc taatctcttt cgagctaccc aaacagaaga aaaactcaag cgggatagcg
     9961 tgaattcaaa acagcaagct aataccacac actttgacgt gggtagcaag gtgaggcaaa
    10021 ccattcagga acttggcgga accatacctg aggagttgcc gaccccgcag gtcagcatca
    10081 agcagttgga aaacagtgta aaaattacag agaagaaata gcagaaacga aaaactcttt
    10141 ttgttgattg agacacccga tgcgtaagcc aaggtccagg tgcagtgaac acaatggcta
    10201 aacattgcac ctttcttttc ccccctcagt tttaacctat tttttcttat gcattttctc
    10261 agacaagaag tcagaagaat gcatctacgc tacagaaaat agcgatttca cataactaaa
    10321 agttatcaat ttcctctcac cttaaaaaat atcaatcgtg acaatgcgca caaatcgcta
    10381 ccctgccaga cagattttta gggagagaac catgctgtta cacattttgt atttggttgg
    10441 cattactgcc gaagccatga cgggggcgct ggcggccggg cgacggcgca tggatacatt
    10501 tggcgtaatt attattgcaa ccgccaccgc aattggcgga gggtcagtgc gcgatattct
    10561 gctgggccac tatccgctcg gctgggtcaa acacccggaa tatgtgatta tcgtcgctac
    10621 cgccgcagta ctcaccacta tcgttgctcc tgtaatgcct tacctgcgca aagtgtttct
    10681 ggtgctcgat gcgctcggac tggtggtctt ttctatcatc ggcgcacagg ttgcactgga
    10741 tatggggcac ggcccaatta ttgccgttgt cgcggcggta accaccggtg tatttggcgg
    10801 cgttttgcgc gatatgttct gtaaacgcat tccgctggtc tttcaaaaag agttgtatgc
    10861 cggtgtctca tttgcctccg ccgtgctgta catcgcgctg caacactatg tttctaacca
    10921 tgatgtggtg attatctcca ccctggtatt cggctttttt gctcgtttac tggcgctacg
    10981 tcttaagctg ggattaccgg tcttttacta cagccacgaa ggtcactaag gttcaaaacc
    11041 tgtgatctgc tgggcagcca gccaactgcc cagcttcttg atttgcgcat tttccttcca
    11101 ttcaataacc tgtctggcgc gcccagatcc agtccccggc agctgctgcc agaactgctc
    11161 cgtgctaaat aaaagttgcg accaggaccg ttcatcactg gcattaagcg ccgcccgggt
    11221 tagcggtatt cccattgcca tcacccagcg agtaaaaggc tggttacgag ccagattaaa
    11281 ctgatgccat aactgcgcac ttttactttt cgcgatcccc ggcgtgttct gtaattgctc
    11341 tggcgttaat aaaagccagg aaaagatatg ctcaaagcga tgagtctgat gcagcgcgcg
    11401 ccaaccggcc tcaccaatgc catccagccc cagaacctgt tttgacccca gccagactaa
    11461 gcgtgaaatg aactgttcct gacaaacatc agaagcaaag tagcaggtca acgggttaaa
    11521 gcggttttct ggtggtgtcg gttttgtacg ttctgcaccg cgccagacca catcatcaat
    11581 gcgaggaata ccctgaccgg caaggctgac gaggatctga tcaccaggcg caatatccca
    11641 ctcctgccag cgcctgacgg aaccaatatt cacccgctgg acttttttat catccagcat
    11701 gacaggtgcg agtgacgcaa ccaccgatat tttaccgctc ttacccaccg caaactgaat
    11761 cgccttcact tcggcaacct gagctacagg ttgatatttc caggccacca gccactccgc
    11821 ctggcccggt agccaatgac gggattcggg ttccttagcc cctcgcacaa ccacgccatc
    11881 ggtgacgaag ggtaatttcg ctttccacca ctcattgcgt acgcgcgcaa cctcatcagc
    11941 atttttaacc gcacgggtat acctctgcgt tagagtaaaa cctgcggtag ccagctcctt
    12001 taaacgatcg gtcattaact gcggtccatc cggccatgcc cagacaaaaa cagccagtga
    12061 gtttagcgtg tcgctattcc cctggcgcat catcaggcca gcaacttttg cgcgggcatt
    12121 tattcccccc atttgttgtt ggatatgccc ctcgcgctgg agaaatattt ccccctgaag
    12181 cgtactgttg gctaaaggcc cgctaacggt ttgcagcaca gcggaaatta agctaacttt
    12241 ctgcgtccag tcctcgcctt tcaggccatt accgcgactg attgctttgt tcagtttccc
    12301 gtcccgataa accagggtta ccgcaacgcc atcaactttt ggctgcaccc aaagatcgct
    12361 acgttctcgc atccacagac ttaatgcgtt tttatccgcc attttgcgta cacccgtatg
    12421 ggcaaccgga tgcataaccg caccatttaa aggcggcatc atcacgtcgc ggggctcgct
    12481 cacaaagcag cgttgccact gcgtaagacg ggcgcttaac tgatcgtaaa caccgtcttc
    12541 cacctcactt tttccttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag
    12601 gcgggaaatt tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga
    12661 tgattgccag cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc
    12721 tgtagtcagg ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag
    12781 tctgcgagga cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa
    12841 acagctacgc aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg
    12901 gccgcgccgt gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga
    12961 tactcatggc tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca
    13021 gcctgattca ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac
    13081 acaccacacg ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc
    13141 atgatgaatt taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg
    13201 gtaattacta tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg
    13261 tttttctcga tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc
    13321 ggagtatctt tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg
    13381 gtcaggacag cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc
    13441 attacgccga atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt
    13501 tgaagaccat tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg
    13561 ctttaatcag caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc
    13621 ttcacctgtg gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa
    13681 ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag
    13741 gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg
    13801 aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc
    13861 aagagctgga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat
    13921 cacaaagcgg gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc
    13981 tgccggaaga ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg
    14041 aggggcaaac acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca
    14101 ttctggccga gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga
    14161 ttgaagatac tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg
    14221 agctggtaga gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc
    14281 aggccgaaaa ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca
    14341 tcaaacttgc cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac
    14401 gtcgccgcat cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta
    14461 tccaccacat taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt
    14521 atcgcgtaat caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa
    14581 aaatcctctc ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg
    14641 gtcgcgagaa gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc
    14701 actcaatcat ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc
    14761 gcgtgctcgg gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca
    14821 tcgccattcc aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg
    14881 gtgtgccggt tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg
    14941 ttgccgcgca ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg
    15001 cccagcgctg gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat
    15061 ttatcgagag cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag
    15121 ggcgaattgt cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata
    15181 ccgatatcgg tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc
    15241 agccgcttac cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg
    15301 ccgcgtggct gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga
    15361 aaaacctcaa gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg
    15421 gtggtagccg taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca
    15481 tgaagctggc aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg
    15541 tggtggtcgc gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc
    15601 acggacatct gcccattaaa ggtgccgatg gcgtactgat cacctttgcg aaatgctgcc
    15661 gccctattcc tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc
    15721 accatgaatc ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg
    15781 tggaatggga taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca
    15841 atcatcaggg cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc
    15901 aaagtttgaa tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg
    15961 ctcgtgaccg tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga
    16021 ttaaagtcac ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct
    16081 gcgaaatgct cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac
    16141 ctcataacgt ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg
    16201 ccgtctggcc tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct
    16261 gggtacaggt gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg
    16321 gcatgcagat tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt
    16381 acactcgccc gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat
    16441 tggccctggc ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg
    16501 tttccgttgc ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca
    16561 tgtacctgcg tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag
    16621 gcggctatcc ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca
    16681 atcagcaagg cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt
    16741 aagtgccatg aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg
    16801 cgcagcactt agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt
    16861 acaccttccc ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc
    16921 gggcgtttat gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg
    16981 gcggatgatg acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa
    17041 tttcagcgcg gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga
    17101 agcaaagcgc ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga
    17161 tctcagcacg ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt
    17221 aaagcaggcc acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc
    17281 cattgaagaa ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt
    17341 gcgcactttg caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca
    17401 tccggcgcaa cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc
    17461 cttacgtgcc ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa
    17521 aaataaactc ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc
    17581 ggagatcgag catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt
    17641 aggttccggt aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa
    17701 acaggtggca ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg
    17761 caactggttt gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa
    17821 agcgcgactg tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac
    17881 acacgccatc ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga
    17941 acagcatcgt tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg
    18001 cttccatccg catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac
    18061 ggcgtatgcc gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt
    18121 gactacggtc gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc
    18181 ctgcataact gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt
    18241 actggaagcg caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt
    18301 gaatgttggc ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc
    18361 gtttaaacaa ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga
    18421 tgtgcctaac gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt
    18481 acaccagctg cgcgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta
    18541 caaaacgccg ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga
    18601 cggttttgtg attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac
    18661 gcgtcagacg ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat
    18721 cccggaagtt cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc
    18781 cttgatagaa cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt
    18841 gagaagaccg ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa
    18901 tcaccagcgc attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg
    18961 ccatgtgcga cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg
    19021 ttgcaccgag gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc
    19081 ccatcatgcg ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca
    19141 gaatcgccag catcggcagc gccagcgaag ccagctccca cagtttcagc cccatcaacg
    19201 ccatcgccag gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga
    19261 cgcggtaaaa gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac
    19321 agacgaaggt cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca
    19381 ccgtcaggca gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc
    19441 gtcccacatc cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg
    19501 gcgtggtgga gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca
    19561 ccagaccgaa cgttgcacag gccatcgcca cttccgtcgc attggtgaag ccataacgtt
    19621 caatgaacaa tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac
    19681 cggccaacag ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg
    19741 cattttgcat caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac
    19801 cggcacgcaa actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta
    19861 acggatcgcg cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta
    19921 gtagcgccag cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca
    19981 aaaaggagac ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa
    20041 gcgttgctaa agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca
    20101 tactcgtcat acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat
    20161 gctttcctgg gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa
    20221 aataccaaaa gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt
    20281 ggcaaacgtt tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt
    20341 gttttcgatg tctgtttcca ccctcgagtc agaaaatgcg caaccggttg ctcagactca
    20401 aaacagcgaa ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc
    20461 cgcctgtcag catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg
    20521 ccaggcgctg ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc
    20581 ctccggtgtg gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt
    20641 gtctattcag ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct
    20701 gaaaaccggt ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct
    20761 ggcaagttgc accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac
    20821 gccgctggtt tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct
    20881 gacctccatt ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa
    20941 tctgctgctg gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc
    21001 ttacttacgc gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt
    21061 tatgggcatg ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac
    21121 gccgctctat tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt
    21181 tatgatcact tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca
    21241 gccagtgtcc ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct
    21301 gaactcgttt gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa
    21361 cggagtgatc cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat
    21421 gttgatcgtg ctgggtctgt tcccggcagt gagcggtttt gtacaacaca ttccagaacc
    21481 ggttctgggc ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat
    21541 cgtttctcgt gagccgctga accgtcgggc gattctgatt atcgcgctgt cgctggcggt
    21601 tggtctgggc gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa
    21661 cctgctctcc tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt
    21721 cccaccagaa aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc
    21781 tctttcacca ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca
    21841 tttcacggga tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc
    21901 tctgttagtg gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca
    21961 tatcagcgca tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca
    22021 ccgtttttcc gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca
    22081 gcccgcgccc ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga
    22141 accacgccat gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac
    22201 cgcgccgcta ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc
    22261 gaatagcgaa tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc
    22321 agaagccggt aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc
    22381 gctcaatgat gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt
    22441 tacgctgact aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg
    22501 taacgccgac ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag
    22561 cgaaaaatcg ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg
    22621 tcgcttggaa gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga
    22681 tctcagcttg cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact
    22741 gtcgatgaac gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa
    22801 cgcggaattt tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg
    22861 tatggtcaga acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc
    22921 ggcaattgcc gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac
    22981 gacacccggt tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat
    23041 cattgatatc gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa
    23101 cctgacgctg gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc
    23161 cgccgccgcg acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa
    23221 cagcagcacg gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc
    23281 caccgcaagt gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg
    23341 tgtgccggtg aatattttgc aacaatgggg atggcctgaa ttaccgttga ctggcgacgg
    23401 caatattcag cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt
    23461 tagcgggcaa ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc
    23521 gggcgtcgtt tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc
    23581 gccgggtacg actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc
    23641 gcattccctt gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc
    23701 agaccattta ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca
    23761 ccagtcacag taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg
    23821 tcagcagcag gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat
    23881 gccgtgcctt cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga
    23941 gtgttatcac gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga
    24001 ctaccgtcga gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac
    24061 tgcacatcgc ccgcttcagt gaacaccggc gcaaccatca cattgtcgcc taacatgtat
    24121 tgacggtcaa ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc
    24181 atcggcgtac cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg
    24241 catttcagtt gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc
    24301 acacgataag atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac
    24361 cagcgtttgt aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag
    24421 aagccaaaac ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg
    24481 tagttagcgt aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc
    24541 gagcgggcaa acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc
    24601 agttcgttgt agatgtacgc ataatggtta tgcattttct gcgaatcgga accgtcaaac
    24661 cactgaacat caattgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc
    24721 gcgaccagac ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca
    24781 taaatcgcca gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg
    24841 agtaaatagc ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg
    24901 ttaatccaga cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg
    24961 tcagggaaag tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa
    25021 cagtcaaagt ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg
    25081 tttaccgtcg cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac
    25141 cacgggggcg gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc
    25201 ggcgtcgggc cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact
    25261 ttggagactt tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc
    25321 ccataaccac ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg
    25381 ccgccgtccc ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc
    25441 tctcccagac cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg
    25501 cgttgattat tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt
    25561 tcgccgttgc gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag
    25621 ttgccacttt taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc
    25681 aaaatattga gcggataatg aggaccgtta ttcagcgccc cctgaaaatg ctcaatccgc
    25741 acaccgacaa taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc
    25801 tgccaggtac gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc
    25861 tgttcaacct cgaatacctg aagcgggtga atcaaattga ggccaggttg aatcaaccag
    25921 tttccatcgc taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt
    25981 tgctggcaac ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc
    26041 gtgagtgagt agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca
    26101 atcgtgaata gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca
    26161 tcatatccgc cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt
    26221 ttgagcacaa acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac
    26281 tcgccgtagt cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc
    26341 aacacaccaa tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag
    26401 aacatcgcga ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta
    26461 catttccagt cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa
    26521 gtggtgagaa aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc
    26581 atcgccccac cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc
    26641 cgccactggt cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt
    26701 ggtgcttcaa cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat gaatgccacc
    26761 acggaaagga ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa
    26821 ttaaccagtg gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa
    26881 cgccaggatt gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg
    26941 cagtaaggga tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc
    27001 atagatcatt ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat
    27061 cccgaatggc agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt
    27121 tcgatcggcc aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa
    27181 aaacatggtt ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat
    27241 atataacatt acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat
    27301 tttctcttta acggacaaca cttcactcct catctgcttt tccttgatgc tataggggtg
    27361 ctgagaccga taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg
    27421 ttattaattt atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc
    27481 attgtaaaat atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat
    27541 taggcggaaa aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc
    27601 cgcctccatg tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt
    27661 caaatccagt tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca
    27721 gtttacctct tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag
    27781 tatttatttg tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta
    27841 ctttaggggg tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat
    27901 acataacaac ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg
    27961 ttagttactg gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca
    28021 tggcattaac gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc
    28081 tgatggcggc ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa
    28141 gtaccgcttt gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct
    28201 agccgatgct cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc
    28261 aggggaggca aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga
    28321 ggctattgct cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga
    28381 tgaaatcatt gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc
    28441 cgaaatcaaa ccaatgaagt tactggaagc actgcgtaaa atggaaaaac gtggagctct
    28501 ggagaaaatg cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac
    28561 tggccgggca gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa
    28621 agtacatttt ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg
    28681 ttataccgga agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg
    28741 aacacaagaa ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga
    28801 gatcccggca gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt
    28861 cattatgcta tttaagcagc ttgaaccaat ctcaaaacat catcccctag tctttatcgg
    28921 tagaaatgat ccccgcaaac caattagtaa ggaaagcatt aaccaagtaa ttgaattact
    28981 tggatataag ggaagactca cagggcacgg tttcagacac accatgagta caattctgca
    29041 tgaacaaggc tttaattccg cctggattga aatgcagtta gctcatgtgg ataaaaattc
    29101 catcaggggt acctataatc atgccctgta tctcgatggc cgccgtgaaa tgatgcaatg
    29161 gtacgctgat tacattgatt cactctccag ccgggaaagt taagtcagaa atacttttcc
    29221 ctgatgtttt cctcaattaa gtattttatc ctctggccta caaggctgga ggatttttgt
    29281 tattatatta tttttttgcg tcttcaaact tattacaaaa acataattaa aaacaaattg
    29341 acatttttaa attggcaatt acaaaataca gccttgcagt tatttcgata cctttagcaa
    29401 aggaatagtt gattcagata taatttagca tttcaatcat ctgacatcat tataaaaaat
    29461 gagattcaca taaagataaa attattgttc tcaaggaaag catgaagggg cttcgtaagc
    29521 aatattcatt cggtgccaag acaggacttg atacacagaa tctcaacttt tatgctctca
    29581 gactatatca ccacatgttt tacaggctca aaaaatgaac gatagattat gctttgaagt
    29641 gcacgataac aagggttatt tcgtatttcc ggatacctgg tttggcccat tgctggggga
    29701 gtttgaagaa gtgcttgatg catacgacgc tgatgaaatc agcgagacga gttacatcaa
    29761 caaactccga cgccttgctc aacgggagcc ggactttatt gatatacacg ctcatctggc
    29821 atatgcattt ctggaacaga atgccccgcg taaggcactg aacgccgctc ttaaaggact
    29881 ggcggcaggt aatcgtatca tcccggaaag tttctgtggt gaaataatct ggatgcatcc
    29941 cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca
    30001 gcgccatcag gatgcggtta tgctcactga taaaatactg gcatacaatc ctgaagataa
    30061 tcagggggct cgctggttgt tgggttcaga gctgttacgc accggcgatc atgagcgagc
    30121 cttcagtgta ctgaaagagc atgctgatga attttcacct tactggtatg aactcggtct
    30181 cctgcatttt ctgaacgggg aacatgttaa ggccgccaca gcttttcgcc atggctttgc
    30241 cacaaacact tacatcgcag aaatgctgtg cggaaatctg catccatttc cacttgctgt
    30301 gtggcatgac ttctccggct ctctggatac ggcagaagat tactatgcaa catactcacc
    30361 attatgggga caatatccgg aagcattgct gtttgtaaac tggctctata accattcctc
    30421 cgtgctgcat gaacgatcag aaatcattaa atgcgccgaa atgctgattc aggaagatga
    30481 tttcgaaata tgcgaaagta ttttaagaca gcaggagcac ctgtggaaaa gaattgacaa
    30541 gacactttct gaggaaattg tacagaaatg cagaaatatg aacggtgaat atatctggcc
    30601 ctggatattg cccttttccg cagcagggat gaaacattca agcatccagt atcagtagat
    30661 attgcattag tgtaacttta aaacaagtaa taatttatta tccaataata acactgtgtt
    30721 ccttccccag gtgattaccc tggggttatt ttctgaaagt ttcctttgca aaaggatagc
    30781 gttgtaaatt actttaacga attactccat gaactcatta aagttctttc gtaatacttt
    30841 atcgtcaaac acgttatagc tggcatcaaa aaccacagat ttaccctgcc agcgggtaat
    30901 cccctcctct ggcgttttcg gttgaactgg tatccccgtc caacacaggc agccattata
    30961 aacagtaaaa aattgtattc gctgttatga aatcaatagt cctgacgtac tgttgaatgt
    31021 gcttgtgttc tgaacctcgt acaaccagta catttgatat cgaattataa acaaaacatt
    31081 gcatccaact tttttaatat ggacaatagt tgtccatacg gtggttttat attcgataaa
    31141 tcaatggatt tcattgagtc acaggactgg taatggatga aaatgcttta aggtttgcct
    31201 catactggcg caactcgctt gcagatgctg agtcaggaaa gggctgtttt gaacggaaag
    31261 acgcccaaaa tttcactcac tggcatggga tagcggcggg acgtcttgac gaagcgattg
    31321 tcaataaatt ttttaaggga gaaaaagacg atgtcgaaac tgtcaatgtc atcttacgcc
    31381 caaaagttta tttccggtta ctgcagcatg gtaaggaccg ttccgcaggc acgcctgata
    31441 ttgttacacc gatagtgacg ccagccttgc taagccgtga gggtttttta tatccgacgc
    31501 cagcgacctc cattcccaga gacctgcttg aacctttgcc aaaaggagca ttttcgattg
    31561 gtgagattgg gcagtatgac aaatacaaga cgacccatac tgatcctacc cacgtaatgt
    31621 ggacacggcc ctaagcgaga ttatggtttt caaattgttc cgggctgaga ccgccacagg
    31681 cactgtgacg acgccagcga ttgtaatcac actcgatata attaaacacc gttgcccgca
    31741 ttatttcccg gctgctaaag cgttccccgt ggatacattc caccttcagc gaatgaaaga
    31801 agctttccac acaggcattg tcataacagt agcctttcgc actcatactg ccacgcaggt
    31861 tgtgtcgctt cagcattgcc tgataatccc ctgaacagta ttgaccacca cggtccgtat
    31921 gaacaatgac gctttccggg cgtcttctcc gccacaacgc catttgcagt gcatcacagg
    31981 ccagttgtgc tgtcattcgc ggtgacatcg accagccaat gacggcgcgt gaccacaggt
    32041 cgatgactac tgcgagatac aaccagccct catcggtacg caagtacgtg atgtcacccg
    32101 cccacttctg gtttcgggcc actggcgctg aagtcctgct ccagcagatt ctccaatacg
    32161 ggcaggccat gtgcacggta gctgaccggg ctgaacttcc ggctggcttt cgcccgcagc
    32221 ccctgacgac gcaggctggc ggcaatggtt ttaatattga actccggcag ttcgtcagca
    32281 aggcggggag caccgtatcg ctgctttgcc tcaaagaatg ccttatggac agcggcatcg
    32341 caggtgagcc gaaactgttg gcgcaggctc atctggtgac gacgcctgag ccagacatac
    32401 cagccgctgc gggcaacccg aagtacacga cacatcgctt tgatgctgaa ctctgcccga
    32461 tgattttcga taaagacata cttcatttca ggcgcttcgc gaagtatgtc gcggcctttt
    32521 ggaggatgga cagttcctca gcctgctccg ccagttgtcg tttaaggcgg acattttcag
    32581 cggccagttc gctttcgcgc tctgacgaac tcatttgttg ctgctgttta ctgcgccagg
    32641 cataaagctg agattcatac aggctgagtt cacgggctgc ggcggccaca ccgatgcgtt
    32701 cagcgagttt cagggcttcg ttacgaaatt caggcgtatg ttgtttacgg ggcttcttgc
    32761 tgattgatac tggttttgtc atgagtcacc tctggttgag agtttactca cttagtcctg
    32821 tgtccactat tggtgggtaa gatcaccctc cattaaaact aaaacgggcg ctttctgata
    32881 tatcatctgt aagcgcagta ataccattag tagcacctcg cgcattgatt attgaatatt
    32941 ataactggaa tgttatattt cttgttatga tgctgttcag tattataata atgttattat
    33001 caccaaagat aactcatgca aatgagaaag taatatgtca aaacagtgca tcctttgtga
    33061 ccaaagagtt tatctgcggc actatacttg tctcattaat actcagttct ctattttgct
    33121 acatatcaat atcaccatca attttcatgc atacttatgg cctgagtact tttagttact
    33181 caattatttt ctccttctca gttttatttt tcataatcgg aaatcaatta tcaaagattg
    33241 aaaaattatc caacctatgg tttttattac ccaatagcca attttatcgc attacaaata
    33301 aatggttcaa aaacaggatt tgccgcatct attacaggtt ttactcaaac aataagcgct
    33361 ggtacaatca gtttcctgtc agtaaccgta agcgacaacg gtgttggtcc cggaactact
    33421 ttaggtcttg gttcactaat aacttcatta ctgtcaatag ccgtaatcgc cattgcagga
    33481 aataataaaa agtaagtgta ctctgactgc gt
//
LOCUS       DAJTUM010000038        33260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTUM010000038 DAJTUM010000000
VERSION     DAJTUM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(3..299)
                     /locus_tag="OO471_001922"
     CDS             complement(3..299)
                     /locus_tag="OO471_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6243206.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            514..1242
                     /gene="tonB"
                     /locus_tag="OO471_001923"
     CDS             514..1242
                     /gene="tonB"
                     /locus_tag="OO471_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6243207.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1282..1680)
                     /gene="yciA"
                     /locus_tag="OO471_001924"
     CDS             complement(1282..1680)
                     /gene="yciA"
                     /locus_tag="OO471_001924"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6243208.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1785..2324)
                     /locus_tag="OO471_001925"
     CDS             complement(1785..2324)
                     /locus_tag="OO471_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6243209.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2354..3097)
                     /locus_tag="OO471_001926"
     CDS             complement(2354..3097)
                     /locus_tag="OO471_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6243210.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3125..3214)
                     /locus_tag="OO471_001927"
                     /pseudo
     CDS             complement(3125..3214)
                     /locus_tag="OO471_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3454..4092
                     /gene="ompW"
                     /locus_tag="OO471_001928"
     CDS             3454..4092
                     /gene="ompW"
                     /locus_tag="OO471_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6243211.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4152..4658)
                     /locus_tag="OO471_001929"
     CDS             complement(4152..4658)
                     /locus_tag="OO471_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6243212.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4704..5204)
                     /locus_tag="OO471_001930"
     CDS             complement(4704..5204)
                     /locus_tag="OO471_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6243213.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5290..5469)
                     /locus_tag="OO471_001931"
     CDS             complement(5290..5469)
                     /locus_tag="OO471_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6243214.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="OO471_001932"
     CDS             complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="OO471_001932"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6243215.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="OO471_001933"
     CDS             complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="OO471_001933"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6243216.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7860..9221)
                     /gene="trpCF"
                     /locus_tag="OO471_001934"
     CDS             complement(7860..9221)
                     /gene="trpCF"
                     /locus_tag="OO471_001934"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6243217.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="OO471_001935"
     CDS             complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="OO471_001935"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6243218.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="OO471_001936"
     CDS             complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="OO471_001936"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6243219.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12401..12494)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="OO471_001937"
     CDS             complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="OO471_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6243220.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12653..13534
                     /gene="yciV"
                     /locus_tag="OO471_001938"
     CDS             12653..13534
                     /gene="yciV"
                     /locus_tag="OO471_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6243221.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13531..14151
                     /locus_tag="OO471_001939"
     CDS             13531..14151
                     /locus_tag="OO471_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6243222.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14252..15124
                     /gene="rluB"
                     /locus_tag="OO471_001940"
     CDS             14252..15124
                     /gene="rluB"
                     /locus_tag="OO471_001940"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6243223.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="OO471_001941"
     CDS             complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="OO471_001941"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6243224.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15751..16509)
                     /locus_tag="OO471_001942"
     CDS             complement(15751..16509)
                     /locus_tag="OO471_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6243225.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16729..17778
                     /gene="sohB"
                     /locus_tag="OO471_001943"
     CDS             16729..17778
                     /gene="sohB"
                     /locus_tag="OO471_001943"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6243226.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17814..18065)
                     /locus_tag="OO471_001944"
     CDS             complement(17814..18065)
                     /locus_tag="OO471_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6243227.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18445..21042
                     /gene="topA"
                     /locus_tag="OO471_001945"
     CDS             18445..21042
                     /gene="topA"
                     /locus_tag="OO471_001945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6243228.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21252..22226
                     /gene="cysB"
                     /locus_tag="OO471_001946"
     CDS             21252..22226
                     /gene="cysB"
                     /locus_tag="OO471_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6243229.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22521..22685
                     /locus_tag="OO471_001947"
     CDS             22521..22685
                     /locus_tag="OO471_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6243230.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22688..22855
                     /locus_tag="OO471_001948"
     CDS             22688..22855
                     /locus_tag="OO471_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243231.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22969..23064
                     /locus_tag="OO471_001949"
     CDS             22969..23064
                     /locus_tag="OO471_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243232.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23228..25903
                     /gene="acnA"
                     /locus_tag="OO471_001950"
     CDS             23228..25903
                     /gene="acnA"
                     /locus_tag="OO471_001950"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6243233.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25967..26557)
                     /gene="ribA"
                     /locus_tag="OO471_001951"
     CDS             complement(25967..26557)
                     /gene="ribA"
                     /locus_tag="OO471_001951"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6243234.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26727..27491
                     /gene="pgpB"
                     /locus_tag="OO471_001952"
     CDS             26727..27491
                     /gene="pgpB"
                     /locus_tag="OO471_001952"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6243235.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27640..27948
                     /gene="lapA"
                     /locus_tag="OO471_001953"
     CDS             27640..27948
                     /gene="lapA"
                     /locus_tag="OO471_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6243236.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27955..29124
                     /gene="lapB"
                     /locus_tag="OO471_001954"
     CDS             27955..29124
                     /gene="lapB"
                     /locus_tag="OO471_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6243237.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29317..30054
                     /gene="pyrF"
                     /locus_tag="OO471_001955"
     CDS             29317..30054
                     /gene="pyrF"
                     /locus_tag="OO471_001955"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6243238.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30054..30380
                     /gene="yciH"
                     /locus_tag="OO471_001956"
     CDS             30054..30380
                     /gene="yciH"
                     /locus_tag="OO471_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6243239.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30506..30724)
                     /gene="osmB"
                     /locus_tag="OO471_001957"
     CDS             complement(30506..30724)
                     /gene="osmB"
                     /locus_tag="OO471_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6243240.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30993..31742)
                     /locus_tag="OO471_001958"
     CDS             complement(30993..31742)
                     /locus_tag="OO471_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6243241.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31832..32005)
                     /locus_tag="OO471_001959"
     CDS             complement(31832..32005)
                     /locus_tag="OO471_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243242.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(32153..33260)
                     /locus_tag="OO471_001960"
                     /pseudo
     CDS             complement(32153..33260)
                     /locus_tag="OO471_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         8394 a         8091 c         8524 g         8251 t
ORIGIN      
        1 actcagaaca ctttcttaaa tggtttcact gaaacgtgtt catagactcc tgccgctacg
       61 tacgggtcag catcggccca ggcctgagct gcttccagcg attcaaattc agcaataacg
      121 gttgagccag taaatcccgc agcccctgga tcgttactgt ctaccgctgg cattggacca
      181 gctgtcaaca aacgaccttc atcatgcagt aactgtaaac gtgctaaatg tgccggacga
      241 acggaaaggc gtttttcgag gctatcagct ttgtcttgag cgtaaataac atacaacacg
      301 ggcacaactc cttgttcggg aaagttgtaa agtacgttat ttgaaagggc gaagatctgc
      361 aacggaaaga tgatgtcttt gttaaggtca tgcataaagt aagggtaatt acgccaaaaa
      421 tgacattttc actgatcctg atcgtcttgc cttattgaat atgattgcta tttgcattta
      481 aaatcgaggc ctagtttttc tactgaaatg attatgactt caataaccct tgatttacct
      541 cgccgcttcc cctggccgac gttactttcg gtctgcattc atggtgctgt tgtggcgggg
      601 ctgctctata cctcggtaca tcaggttatt gaactacctg cgcctgcgca accaatttct
      661 gtcacgatgg ttgcgcctgc tgatctcgaa ccgccacaag ccgttcagcc gccaccggag
      721 ccggtggtag agccagaacc ggaacctgag ccgatccccg aaccgccaaa agaagcaccg
      781 gtggtcattg aaaagccgaa gccgaaacct aagccaaaac cgaagccggt gaaaaaggta
      841 caggagcagc caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa
      901 aatacggcac ccgcgcgccc cacatcaagt acggcaacgg ctgcaaccag caagccggtt
      961 accagtgtgg cttcaggacc acgcgcatta agccgtaatc agccgcagta tccggcacga
     1021 gcacaggcat tgcgcattga agggcaggtt aaagttaaat ttgacgtcac gccggatggt
     1081 cgcgtggata acgtacaaat cctctcagcc aagcctgcga acatgtttga gcgtgaggtg
     1141 aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg gattgtggtg
     1201 aatatcctgt ttaaaattaa cggcaccacc gaaattcagt aagcagaaag tcaaaagcct
     1261 ccgaccggag gcttttgact attactcaac aggtaaggcg cgaggttttc cttcaggatc
     1321 aaccgctaca tacttaaata atgcttctgt cgctttatag cgttgcccga ttggttcaga
     1381 cgctactttt ttcacccaca cttcaatatt aatgctgacc gatgtcgtcc ctttctggac
     1441 acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga aagtcattcc
     1501 ttcaacccgc acagtcacta cgcgaccgtg ggcaatttct tttgccagaa tagcgccgcc
     1561 aatatccatt tgtgacatta accaaccacc aaagatgtca ccattggcat tggtatcggc
     1621 gggcatggct aaagtacgta aaacaagatc gccctgaggg acgttatgtg ttgtagacat
     1681 ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct actatgattt tcctgaggag
     1741 aacagaggag atacagcgcc agccccgaag gactggcagt ctggttagga tttatcttcc
     1801 tgcggcatgt ggcggtagat atagataccg cttaacaatg tgaagattaa ggtaagggcg
     1861 gtcaggccaa agactttaaa gttgacccaa atattttgcg gcagccagaa tgcgatgtag
     1921 atgtttgcca gaccgcaaag gataaagaaa acagcccagg ccagatttag cttcgaccat
     1981 accggttgcg gcagcgtgag ttctttactc agcatccgct gaattagcgg ctttttcatc
     2041 acccattggc tgactaacag ggctcccgca aacagagcat aaatgactgt aaccttccat
     2101 ttaataaact catcattgtg gaagaacagc gtcaagccac cgaagacgac caccagaaca
     2161 aaagtgatca gggccatctt ctcaacctta cgaaagcgaa cccagctata gataagcaca
     2221 atcgccgtgg cgacgatcag cgctgcagta gccgcataga tgtcataaat cttgtaaaac
     2281 gcgaaaaaga caaccagcgg taaaaaatca agaaactgct tcattttacg attccgtaat
     2341 caaatgctta aggttattgg cgaatcaaca tatacaggcg gaacagatag atgagcaata
     2401 tggcagaaat caggttgctc aaggtgttcg ccagtacggc accaatttcc ggggttaatg
     2461 cggcaaaaga agaggcaaaa agcagcaaca gtgtttttgc cagcaaccag ctcagtactg
     2521 cgggtgccac cagacgcata ttcgcccaag tcagccgcat actgctacgc atcgaggcaa
     2581 aaatgcccat tttgtcctgt accagcatca ccggtgccag ggccagtaaa atggccatga
     2641 taattcccgg aacgaccacc agcatgatgc caatctgtac taaaagggta gtcagaaaaa
     2701 tcagaataaa taacttgggc aatatcggcg cactggcacc aatagctcgc agcgcactga
     2761 ctctctgacc cgcagacacc agctggataa ttaatattac gcctccggcg agaatggcgt
     2821 taccgattaa ttctgaaaaa gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt
     2881 ccggtgacat attctgaacc aggtcgaaca acccactact gccgctaacg ggcacgccgt
     2941 cattgagctg cgcaagctgt gcatcactgg gtgagaaaac atgccctaac accactgtga
     3001 taaacgcaca tagcaacgat accaacagaa tggtcataaa ttgattacgg aagaaatttc
     3061 cggtgtcacg gtatacggac tgcgccgtga tagacatgca ctctccttga gttttatgca
     3121 ggtgttaatt agcgggcaat tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg
     3181 tatggaaaag catatctttg taaagcggag gtaattgctg gccttcaggc gtgggcaaag
     3241 cgcggcaagt ggcgatcgag ttaatatttg cgtagagaaa atatttaaaa attgatttaa
     3301 atcacattaa ccaggattct caatgcaact tctaaattaa tccagatcaa taaatggtga
     3361 attatcatat gtaatgtgat ctatgtagga tcatttgtta ctccaatgta ggtatattcg
     3421 tcacgttttt ataaccataa cgacggagcg gatatgaaaa agttaacagt ggcggctttg
     3481 gcagtaacaa ctcttctttc tggcagtgcc tttgcgcatg aagcaggcga attttttatg
     3541 cgtgcaggtt ctgcaaccgt acgtccaaca gaaggtgctg gtggtacgtt aggaagtctg
     3601 ggtggattca gcgtgaccaa taacactcaa ctgggcttga cgtttactta tatggcgacc
     3661 gacaacattg gtgtggaatt actggcagcg acgccgttcc gccataaaat cggcacccgg
     3721 gcgaccggcg atattgcaac cgttcaccat ctgccgccaa cactgatggc gcagtggtat
     3781 tttggtgatg ccagcagcaa attccgtcct tacgttgggg ccggtattaa ctacaccacc
     3841 ttctttgata atggatttaa cgatcatggc aaagaggcgg ggctttccga tctcagtctg
     3901 aaagattcct ggggagctgc cgggcaggtg ggggttgatt atctgattaa ccgtgactgg
     3961 ttggttaaca tgtcagtgtg gtacatggat atcgatacca ccgccaatta taagctaggc
     4021 ggtgcacagc aacacgatag cgtacgcctc gatccgtggg tgtttatgtt ctcagcagga
     4081 tatcgttttt aattccgcac aaaaacgacc ccgtaatata cggggtcaat aaggacatgg
     4141 tataaagggt attatttctt cgcttctacg ccatcagttt cagaacgaat taaaaatttc
     4201 tcagttgttt gcggaatatg ctgaatcagc cagtcggcca tttgcttttc ctcatttaaa
     4261 atcgcttcga tgatcggaat tgagactgta tcaccggcat tttttgctgc tgctaatagt
     4321 gaggtgtaac aggtgatttc aaattgctcg aagacatatc cgccaataga gcctttaact
     4381 atttcatcag aagggaatat accaccgatt gactgcccaa gcgcagccat tttactcatg
     4441 gaatctttaa tgactgaacg tgaaatgtca ttacgatcaa gaatagtttc cagttgaaca
     4501 atctggtttt tggtttcact aagatgttgt tcaatacgag cgcgtagttc aggataatta
     4561 tctatacggc tggccatgga ttcaagcata gattcggctt gcttttccat tgcgtgggcg
     4621 tcacgtaacc agtcatgata atgttcaata cgattcatga aaatgtcctc tttataaaat
     4681 gactaaaagt taaaattcat atttcaggct ttattttcgg ctttcttatt tacgttatta
     4741 agggccagat cagtcagttt gatgtcggtg gccttttctt cttccagggt ttctttcaga
     4801 agcttcgctg ctttacggta acctaattgt tcagccagcg tcgctaatgt cccgtaactg
     4861 gcaatctcat aatgctcgac tttctgtgct gcggcaatca gtgcggcatc acgcacttcg
     4921 tttttctcgg tactttcgat gacctcatta gcttcttcaa taagaccttc cattgccaca
     4981 catttcatgc gcttaatttt cagatttgat tctgattcca caacttgatc aattcgttca
     5041 atctgtccat gagtttcctc gaggtgcgca tgaaaagcct gacttaattt ttcatttgat
     5101 gttgctcttg cgagttttgc cagtgcccgg gttaattgtt tttctgcgct gtaggtatct
     5161 gaaagcagtt gaataaatac atcttcaatg gtcttcatat tcatattttt ctccagtgaa
     5221 atcactgcga gccatacggc ccgcagatgc aattatcttt atcagctgat attaataatt
     5281 aacggtgaat caggatttgc ctgatttatt accaccgctt tgttgaccgc cttttttacc
     5341 cgcttcagat gcgcgttgcg gatcattttt aaaattaccg ccgctatgct gaccgccttt
     5401 acggcctgcg tcggatgcct tctcacggtc ttcggcgaaa tttcctgaac taccacgatg
     5461 ttcggccatg ttatttctcc cgttgcgttg cattgtttca ttaatatgag tgttgtgtgt
     5521 cgacactcat taaaattagt cactaatgag aattagtcaa attaagcgca acgagaagat
     5581 agagggaaat atattttgag gaacattctg gatatattaa caattacctg aggaatatgt
     5641 gacttagaga aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta
     5701 tattactgtt gggcggaaaa atgacgtaag ttgacgttcg accggggtaa gcgaaacggt
     5761 aaaaagataa atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat
     5821 gccgccagcg gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta
     5881 caaaaacttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
     5941 taacaatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
     6001 gatccggggc ggaaatacca aacccctgca atggaggtgc agcgttgtac tctttcagct
     6061 tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcccgctc
     6121 gtgacagcaa atatgtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat
     6181 cggcatttgg cgggcagatg aagataggtg cgacattatg gcgcaacgcg gcctggcgga
     6241 agggcgcgga ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgactttct
     6301 cgcactgggc ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca
     6361 ggccaatggg aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg
     6421 ccggagtcac acctgccgca aaggcgcgca gagtggcgtt ttgaatggtc gggccatccg
     6481 ccagtgggtc ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg
     6541 tatcgataat tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaacga
     6601 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
     6661 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
     6721 aaggttaacc accagtagct gctctttatc cgggttttcg cgcatcattt tcagcgcatg
     6781 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt
     6841 gaaggcttca agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
     6901 aagatacgcg tgttgtggac cgacagacgg gaaatccagt ccggcggaga tggagtaaga
     6961 ttcttcaatc tgcccgtctt cggtttgcat catcggcgct ttcataccga aataaatgcc
     7021 cacgcgacca tgttttagcg gtgcgccgtg ctcgccagtt tcgataccgt gaccaccagg
     7081 ctccacacca atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc
     7141 attcgaaccg ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
     7201 aatctgcgct ttggtttctt cgccaatcat ccgctgaaac tcacggacaa tggtcggata
     7261 aggatgcggg ccagccgcgg tgcccagcat atagtgcgcg gtttcataac tgccggacca
     7321 gtcgcgtagc gcctcattac aggcatcttt cagcgtggcg gaaccgctat gtaccgggat
     7381 cacttccgca cccattaagc gcatacgaaa aacgttaggc gactggcgtt caacgtcttt
     7441 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
     7501 gccatgctga ccggccccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag
     7561 caacgcctgc cccagcacct ggttggtttt atgtgcgccg ccgtgcagca aatcttcacg
     7621 cttgagatac agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagtgcggt
     7681 tggacgcccg gcatagtttt tcagcaggtc gttgaactgg gcttgaaatt caggatcttt
     7741 ttgcgcgctg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
     7801 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
     7861 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcccggt
     7921 tgcgactcta cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg
     7981 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat
     8041 agtgaccagt caaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
     8101 acgtgctgaa actcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
     8161 acatgtgctg gcagagcttc acgcagcgta tcgatataca gctgatcttc attgccatgc
     8221 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
     8281 ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca
     8341 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
     8401 tcataagctg ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc
     8461 aacacccggc gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga
     8521 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
     8581 ctgattaccg ttacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca
     8641 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc taatgcaatg
     8701 gcgcgctcca gttcctcttc attactgact tcggtcagca cccccatctc cagactgtga
     8761 gcgacggctg caagctggcg atattggtcg tcatccagta ctgaaagcat taataagcag
     8821 gcatcggcct ggtaatagcg cgccagatag atctggtaag ggtcgataat gaagtcttta
     8881 cataaaatcg gctgcggggc gatttggctg acgatgagga ggaaatcaaa gctcccctga
     8941 aaatatttct catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca
     9001 atgcgtgccg gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttacac
     9061 tccagaataa acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg tgtgctcggc
     9121 tgaacctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc
     9181 gccttgtctg cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagc
     9241 gcggtaactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
     9301 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct
     9361 gcttcatggg cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
     9421 tcaggtgttc cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg
     9481 gtcaattgat agctcttaat tttgccgtca tgcagctcgg caacgattgt cggcgcgtgt
     9541 aatgaaactt catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg
     9601 cgcaaggttt cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc
     9661 ggcggatgcg ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt
     9721 tgctggcgaa ccggcatcgc gtggcggaat ccggtgtgat atttcggcgc aaagaggaaa
     9781 catacgccta actcgtccag cgcctggcgc gatttattgg cgttcatatc aagattaata
     9841 ccgaatgccg ccagcagatc cgacgagccg gatttactgg agacgctgcg gttaccgtgt
     9901 ttcgccactt tcagcccaca ggccgcggcg acaaacgcac tggcggtaga aatattgata
     9961 ctgttgctgc cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg
    10021 aacggcgctg cgttttccag tagcgcggtt gctgccccgg cgatctcgtt cgggtgctca
    10081 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc
    10141 accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt
    10201 ttttccagaa tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc
    10261 gtttgttcca gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat
    10321 ccacaaacgc gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg
    10381 atggttaaac cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc
    10441 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
    10501 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt
    10561 ccgaggcaaa tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata
    10621 caaccggctt cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc
    10681 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta
    10741 tgaccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
    10801 agcagaatgt cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca
    10861 gtacagcgcg ggctttgtta cgggtttcgt cggcttccga ctgcggaaca gaatcaagga
    10921 ctacaccagc acccgcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
    10981 caatgcaggt gtcgagatcg ccatgcgcgg tgaaataacc taccgcgccg ccgtagctgc
    11041 cgcggcgacg accttccgcc tcggcaatta actgcatagc gcgtactttc ggcgcaccgc
    11101 ttaacgtccc catattcata caggcgcgat aagcgtgcag ggcgtcaaga tcgtgacgca
    11161 gttcgccgac tacgcgagag acgaggtgca tcacatagga ataacggtca actttggtga
    11221 gatcggcgat gtagcggctg ccaggggtgc aaatgcgtgc cagatcatta cgggcgagat
    11281 caaccagcat cagatgttca gacagctctt tatggtcagt acgcatttcc agttcaatac
    11341 ggctgtcgag atctctgtcc agtgaaccat cggcgcgacg accgcgtggg cgtgttccgg
    11401 caatcgggta gatctcaatc tggcggctgg tggcatcata cttgagcgag ctttccggcg
    11461 acgcgccaaa tagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
    11521 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag
    11581 atggcaccac ctggaaaatt tctccagcgc gaatcgcttt ttgcaacaaa cgcactacgc
    11641 caccgaactc ttcatcgctc tgattacatt cacaacgcat atgcggcacg gaaaccactg
    11701 gcagcggcgg cgcggcttcg gtcagttgct gacgtagttc gttcaggcga gcagtgagac
    11761 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct
    11821 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt
    11881 ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc
    11941 cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgc aataaacgga
    12001 aagcgtcaaa aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag
    12061 gggggaagcg cagcacacgg cagtttggtg attgttcact ttccacaccc gcaggcaggg
    12121 cgttatccag tagtgccagc agggcttcgc cattgccgga aagtgcctga attgtgacgg
    12181 tgtcacctaa tgctgtaatg cgcagcgcac tgtctatcag cagcaggctt tttaaatcat
    12241 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgattc ccacacaact
    12301 ggtgaaaaag cgcagtcggg ttgtcgcgat aagcgccttc gcaggttagc agttcgagag
    12361 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
    12421 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
    12481 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
    12541 tgcgtactag ttaactggtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
    12601 agacccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
    12661 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
    12721 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
    12781 cgaccatgac accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact
    12841 ggcgctgaat cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca
    12901 tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca
    12961 gacagaacgg cgcaatcagc gggcgcagca aattgccgaa agacttgaaa aagcgcaaat
    13021 acctggggcg ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca
    13081 ttttgcgcgt ttccttgtgg agtgcggcaa agccagttca atggcagatg tctttaaaaa
    13141 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc
    13201 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
    13261 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc
    13321 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg ccacacttgc
    13381 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
    13441 cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga
    13501 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
    13561 aacccgcagc aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt
    13621 gtctatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgccatg
    13681 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
    13741 gatctttctg aactctcgac ctattcattc gtggataacg ttgcgtttcg tttgatgaaa
    13801 aacaacacgc cgggcaacta taccttcatc ctgaagggga cgaaggaagt gccacgccgt
    13861 ctgttgcagg agaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag
    13921 gcgttgcttg aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc
    13981 gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg
    14041 attattcacg gcggctatct cgggcagaaa ccaacaacgg ttattgatct taccgatgat
    14101 acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac
    14161 tctgtatact acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac
    14221 gcctgtgaag gcgacaccaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc
    14281 ccgcgctggc cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag
    14341 tgttgatggc aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat
    14401 tcgtatcgat ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct
    14461 ggcctattac aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac
    14521 ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga
    14581 cgttaacacc tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat
    14641 gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga
    14701 cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa
    14761 aaccatcaag ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga
    14821 aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct
    14881 gatccgtgtt cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac
    14941 ggagctggac ctcgcccaga ctaactatct gcgcgaactg gtggagcttc cgccagaaac
    15001 cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg aaggctaatc agattcgtcg
    15061 tgcggtgaaa cgtcacagtc aggtgagcgg cagtcgtcgt tctggcgggc gtaataacgg
    15121 ttaatcaaag tatgccggac gtcatatccg gcatttttac agattaataa tcaatcccta
    15181 tttgcgcctt aacaccggca tcaaacgcat gtctgatggg gcgcagctca cttaccgtat
    15241 ccgccagttc aagaatatcc cgatgacagc ctcgacccgt gataatcacc gtctgttgat
    15301 gtggacgttc atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca
    15361 tatacgtcag ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg
    15421 catgttgcca gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg
    15481 taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc
    15541 cattaggcca ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg
    15601 cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga
    15661 tgataatacc gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc
    15721 gctgctgacg ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg
    15781 ttgggcgtca aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta
    15841 gagcggcatg atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct
    15901 tgcgcgcatt gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg
    15961 atattcatcg gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc
    16021 accccagttg gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc
    16081 gtccgatttg agtaataaag gaagcagtgc ctgggtgagc ataaaggtgg cattaacgtt
    16141 gatctgcatg acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc
    16201 gagcaatccg gcattatgca aaacaccatc cagacgcgga taattaacga caatgcgctg
    16261 tgccagttgt tggcaatttt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg
    16321 ctgacgccca gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg
    16381 gcccaacaga atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat
    16441 cccatcgctg gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg
    16501 gtaatgcatg gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact
    16561 ttggggcttt atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca
    16621 aaatggatac tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc
    16681 ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg
    16741 tctgaatatg gtttgttttt ggctaaaatc gttaccgttg tgctagcgat tgcggcgatt
    16801 gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc
    16861 aacaatctca gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac
    16921 tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa
    16981 gcagcaaaag cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg
    17041 gtgctggatt ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata
    17101 acggcggtac tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct
    17161 ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa
    17221 aacattcctt taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc
    17281 tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg
    17341 gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac
    17401 accgccgggc agtataagcg cacgctgacc ttgctgggtg aaaataccga agaagggcgg
    17461 gagaaattcc gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt
    17521 atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag
    17581 gcggtagaga aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg
    17641 atggaaggcc gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga
    17701 ttcaccggca gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt
    17761 caaaagccat tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca
    17821 accagatgat atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg
    17881 gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc
    17941 acgccattac gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca
    18001 cgaatgattt tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg
    18061 ttcattattt actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa
    18121 tataccacgc tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa
    18181 gttgcgtatc ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt
    18241 gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac
    18301 tcagtcacct gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagagcg
    18361 agtccatatc ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga
    18421 agaatcaaat taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca
    18481 aaagccaaaa cgatcaacaa gtatctgggt tgtgactacg tggtgaaatc cagcgtcggt
    18541 cacatccgcg atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc
    18601 accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg
    18661 gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc
    18721 gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt
    18781 gaccgcgaag gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg
    18841 cgctatagcc gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac
    18901 aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac
    18961 cgcgtggtgg ggtatatggt ttcgccgctg ctgtggaaaa agatcgctcg tggtctgtct
    19021 gccggtcgtg tgcagtcggt ggcggtccgc ctggtggtag agcgtgagcg tgaaattaaa
    19081 gcgttcgtac cggaagagtt ctgggaagtc gatgccagca cgactacgcc atctggtgaa
    19141 gcgttggcgt tgcaggtgac tcatcagaat gacaaaccgt tccgtccggt caacaaagaa
    19201 caaactcagg ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa
    19261 gacaaaccga caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct
    19321 gccagcaccc gtcttggttt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat
    19381 gaagcaggct atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta
    19441 aatatggttc gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg
    19501 aatcagtacg ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac
    19561 gtcaatgtga tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag
    19621 ttaatctggc gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg
    19681 ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gcgctttgat
    19741 ggctggacaa aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttaccggca
    19801 gtcgataaag gcgatgctct gacgctcgtt gaactaacac cagcccagca ctttaccaag
    19861 ccgccagccc gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt
    19921 cgtccgtcta cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta
    19981 gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa
    20041 aatttccgcg agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag
    20101 gtggcaaatc acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc
    20161 cagcagttag ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg
    20221 gttctgacca gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagt
    20281 accggggtat tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc
    20341 accattaacc tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa
    20401 accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat
    20461 ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac
    20521 gagatcgaag agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa
    20581 aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc
    20641 aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg gtgaagtggc accaccgaaa
    20701 gaagatccgg tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg
    20761 cgtgacggtg ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg
    20821 cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt
    20881 tatctggccg atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc
    20941 cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca
    21001 gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt
    21061 ttttataaac aaagggtcgc gaaagcggcc cttttttatt gcatattatt ttttcttcac
    21121 acctatacac taaggctata aatgatatag tggttatagt tagcaccttt tttattatta
    21181 aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg
    21241 gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc
    21301 tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag
    21361 tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga
    21421 cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag
    21481 tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata
    21541 tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg
    21601 agcgttatcc ccgcgtttct ttgcatatgc accagggccc gccgacacaa attgctgatg
    21661 ccgtctctaa aggcaatgcc gatttcgcta tcgccacaga agcgctgcat ctgtatgaag
    21721 atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc
    21781 cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat
    21841 ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa
    21901 cgccgcgtat cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag
    21961 ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg
    22021 tgcgcgttga tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta
    22081 gtactttttt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc
    22141 gtgatgtcgt tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta
    22201 aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc
    22261 gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta
    22321 tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa
    22381 ttccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc
    22441 gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat
    22501 tacaccaatc attcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc
    22561 cttctggaaa tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc tggctggcgg
    22621 tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg
    22681 gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa
    22741 tgaaccagaa acgaaacggg aatgttccgt cgttattcca gacgactggc aactaacatc
    22801 gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc
    22861 atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac
    22921 agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca
    22981 aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa
    23041 ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt
    23101 tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt
    23161 tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg
    23221 aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa
    23281 aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct
    23341 acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcagt
    23401 taccgaagag gatatccacg cactggcggg atggctgaaa aatgcccatg ctgaccgtga
    23461 aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt
    23521 tgatctggcg gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa
    23581 cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga
    23641 tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt
    23701 gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg
    23761 catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga
    23821 cggtgaatgg attgcttatc cggatacact cgttggtact gactcgcaca ccactatgat
    23881 caacggcctt ggcgtgctgg ggtggggcgt gggtgggatc gaagcagaag ccgccatgtt
    23941 aggccagccg gtttccatgc ttatcccgga cgtagtgggc tttaaactta ccggaaaatt
    24001 acgtgaaggt attaccgcca cagacctggt tctcactgtt acccaaatgc tgcgcaaaca
    24061 tggcgtggtg gggaaattcg tcgaatttta tggtgatggt ctggattcac taccgttggc
    24121 ggatcgcgcc accattgcca atatgtcgcc agaatatggt gccacctgtg gcttcttccc
    24181 aatcgatgca gtaaccctcg attacatgcg tttaagcggg cgcagcgaag atcaggtcga
    24241 gttggtcgaa aaatatgcca aagcgcaggg catgtggcgt aacccgggcg atgaaccgat
    24301 ttttaccagt atgttagaac tggatatgaa tgacgttgaa gcgagcctgg cagggccaaa
    24361 acgcccacag gatcgcgttg cactgcccga tgtaccaaaa gcatttgccg ccagtaacga
    24421 actgaaagtg aatgccacgc ataaagatcg cctgccagtc gattatgtta tgaacggaca
    24481 tcagtatcag ttacctgatg gcgctgtggt cattgctgcg ataacctcgt gcaccaacac
    24541 ctctaaccca agtgtgctga tggccgcagg cttgctggcg aaaaaagccg taactcttgg
    24601 cctcaagcgg caaccgtggg tcaaagcgtc gttggcaccg ggttcgaaag tcgtttctga
    24661 ttatctggca aaagcgaaac tgacaccgta tctcgacgaa ctggggttta accttgtggg
    24721 atacggttgt accacctgta ttggtaactc tgggccgctg cccgatccta tcgaaacggc
    24781 aatcaaaaaa ggcgatttaa ccgtcggtgc ggtgctgtcc ggcaaccgta actttgaagg
    24841 ccgtatccat ccgctggtta aaactaactg gctggcctcg ccaccgctgg tggttgccta
    24901 tgcgctggcg ggaaatatga atatcaacct ggcttctgag cctatcggcc atgatcgcaa
    24961 aggcgagccg gtttatctga aagatatctg gccatcggca caagaaattg cccgtgcggt
    25021 agaacaagtc tccacagaaa tgttccgcaa agagtacgca gaagtttttg aaggcacagc
    25081 agagtggaag gaaattaacg tcacacgatc cgatacctac ggttggcagg aggactcaac
    25141 ctatattcgc ttatcgcctt tctttgatga aatgcaggca acaccagcac cagtggaaga
    25201 tattcacggt gcgcggatcc tcgcaatgct gggggattca gtcaccactg accatatctc
    25261 tccggcgggc agtattaagc ccgacagccc agcgggtcga tatctacaag gtcggggtgt
    25321 tgagcgaaaa gactttaact cctacggttc gcggcgtggt aaccatgaag tgatgatgcg
    25381 cggcaccttc gccaatattc gcatccgtaa tgaaatggtg cctggcgttg aaggggggat
    25441 gacgcggcat ttacctgaca gcgacgtagt ctctatttat gatgctgcga tgcgctataa
    25501 gcaggagcaa acgccgctgg cggtgattgc cgggaaagag tatggatcag gctcaagccg
    25561 tgactgggcg gcaaaaggac ctcgtctgct tggaattcgt gtggtgattg ccgaatcgtt
    25621 tgaacgaatt caccgttcga atttaattgg catgggcatc ctgccgctgg aatttccgca
    25681 aggcgtgacg cgtaaaacgt tagggctaac cggggaagag aagattgata ttggcgatct
    25741 gcaaaatcta caacctggcg cgacggttcc ggtgacgctt acgcgcgcgg atggtagcca
    25801 ggaagtcgta ccctgccgtt gtcgtatcga caccgcgacg gagttgacct actaccagaa
    25861 cgacggcatt ttgcattatg tcattcgtaa tatgttgaag taacaagtat ttgcttgccg
    25921 gttattttgc ttccggcaag caaatgaatt acacgatgca agagggttat ttgttcagca
    25981 aatggcccat tttctcggct ttggtatcga gataatgttc gttattgggg ttacgaccta
    26041 caatcaatgg tacgcgttca acaatattaa tccctgcttc ggtcagaatt tcgacttttt
    26101 tcgggttatt ggttaacaag cggacttcat tgacgccaag gagtttgaac atatcagcgc
    26161 aaagagtgaa gtcgcgctca tcagcggcga agcctaactg gtggttagcc tctacggtat
    26221 cgtaaccttg atcctgcagt gcgtaagcgc ggattttatt cagcagacca atgttacgac
    26281 cttcctgacg gtgatacagc aaaataccac ggccttcctc ggcaatttgc gtcaatgccg
    26341 cttcgagctg gaagccacaa tcgcagcgca agctgaacag ggcgtcaccg gtcagacatt
    26401 cggaatggac gcgcgcaagt accggggtat gcccggaaat atcgccatag actagcgcaa
    26461 catgatcgtg tccggttgcc agttcttcaa atcccaccat caggaaatcg ccccatgggg
    26521 ttggcagttt ggcttctgcc acacgtttaa gctgcatgaa attctccaga taatgctggt
    26581 tctgtattgg cttattttgc cataacgaga aggggttcac ctaatcacga cgcgtcgatc
    26641 gttcacggaa tggcacaaat ctgtcaattt ttcttggaac tggcattttc agttatgatt
    26701 gtgggactta tcaaaaagga gaggccatgc gttcgattgc ccgacgtacc gcagtgggag
    26761 ctgcactatt gcttgtcatg ccagtagccg tatggatttc tggctggcgt tggcaacctg
    26821 gagaacaaag ttggctacta aaagcggctt tttgggttac tgaaactgtc acccagccct
    26881 ggggcgtcat tacacatttg attttgttcg gctggtttct ctggtgtctg cgttttcgca
    26941 ttaaggctgc ctttgtatta tttgccattc tggcggcggc aatccttgtt ggacaaggcg
    27001 ttaaatcctg gatcaaagac aaagtacagg aaccacgacc ttttgttatc tggttggaaa
    27061 aaacacatca tattccggtt gatgagttct acactttaaa gcgagcagaa cgcggaaatc
    27121 tagtaaaaga acagttggct gaagagaaaa atattccaca atatttgcgt tcacactggc
    27181 agaaagagac ggggtttgcc tttccttccg gtcacacgat gtttgctgcc agttgggcac
    27241 tgctggccgt tggtttgctg tggccgcgtc ggcgaacgtt aaccattgct atcttgttgg
    27301 tctgggcaac gggagtcatg ggaagccgcc tgctgctcgg gatgcattgg ccacgcgatc
    27361 tggtggtagc tacgttgatt tcgtgggcgc tggtggcggt ggcaacgtgg cttgcgcaac
    27421 gaatttgtgg gccattaaca ccacctgcgg aagaaaatcg cgaaattgca caacgagaac
    27481 aagaaagtta acgctggttg attttccgaa tttaggcctt aaatcatcaa caatgcgcgt
    27541 ggatgccatt tcgcagacgg cgcgaaaatg gtactttaaa gggctattgc ggtaagttga
    27601 ccataattta ttcgctctaa ccacataacg ggaagtaatg tgaaatattt actcattttc
    27661 ttactggtgt tagcgatctt cgtgatttcg gtcacgttgg gtgcgcagaa cgatcaacag
    27721 gtgacgttta attatctgtt agcgcaaggg gagtaccgta tttccacatt gctggcggta
    27781 ttgtttgctg cggggtttgc tatcggttgg ttgatttgtg gcctgttctg gctgcgagtt
    27841 cgtgtttccc tggcgcgcgc tgaacgtaaa ataaagcgac tggaaaacca gctttcaccc
    27901 gcgactgacg tggctgtagt gccgcactcg tcagcggcga aggaataact ttctatgctg
    27961 gagttgttgt ttctgctttt gcctgtagcc gctgcctatg gctggtatat gggccgcaga
    28021 agtgcgcaac aaaacaagca agatgaagcc aaccgcttgt cgcgtgatta cgtagcgggg
    28081 gttaacttcc tgcttagtaa tcaacaggat aaagcggtag acctgtttct cgatatgctt
    28141 aaagaggata caggcaccgt tgaagcccac cttacgctcg gaaacctgtt ccgttcgcgt
    28201 ggcgaagttg atcgcgctat tcgcatccat cagaccctaa tggaaagcgc ctcgctgacc
    28261 tatgaacagc gtctgttggc gattcaacaa ctggggcgtg attacatggc cgccgggtta
    28321 tacgaccgcg cggaagacat gttcaatcag ctgaccgatg aaactgactt ccgcattggc
    28381 gcgctgcaac agttgctaca aatctaccag gctaccagtg agtggcagaa agcaattgat
    28441 gttgccgaac gcctggtgaa gctgggtaaa gataaacagc gcgtcgaaat tgcccatttc
    28501 tactgtgagt tagccctgca gcatatggcc agcgacgatc tcgatcgtgc catgaccttg
    28561 ctaaaaaaag gggcggcggc agataaaaac agcgcccgcg tatccatcat gatgggacgc
    28621 gtgtttatgg cgaaaggaga atacgccaaa gccgtcgaaa gtttgcaacg cgtcatatcc
    28681 caggacagag aactggtcag cgaaacgctg gaaatgttgc aaacctgcta tcagcagttg
    28741 ggtaaaactg ccgaatgggc agaattcctg caacgcgcgg tggaagagaa caccggtgcc
    28801 gatgctgaat tgatgcttgc tgatatcatc gaagcgcgcg acggtagtga ggctgcacag
    28861 gtctatatta cacgccagct tcagcgtcat ccgaccatgc gtgtattcca taagctaatg
    28921 gattaccact taaatgaagc ggaagaaggg cgcgccaaag agagtctgat ggtgctgcgt
    28981 gacatggttg gcgagaaggt gcgtagtaag cctcgttata gctgccagaa atgtggtttt
    29041 accgcataca ccctctactg gcattgtccg tcttgtcgtg cctggtcaac tattaaaccg
    29101 attcgcggtc ttgatggcct gtaattttta aaaaaatccg actttagtta caacatacta
    29161 gttattgatg tcccattgtg ctccggcaac gacggagcag aaaaagcctg ctaggggaaa
    29221 aatcgcaact gttaattttt tattttcacg ggtagaatgc tcgccgttta cctgtttcgc
    29281 gccactcccg gtgcccatca tcaagaaggt ctggtcatga cgttaactgc ttcatcttct
    29341 tcccgcgccg ttacgaattc tcctgtggtt gttgcccttg attatcataa ttgtgatgac
    29401 gcgctgtcct ttgtcgacaa gatcgaccca cgcgattgtc gtctgaaggt cggtaaagag
    29461 atgtttacat tgtttgggcc acagtttgtg cgcgaacttc aacagcgtgg ttttgatatc
    29521 tttcttgacc tgaaattcca cgatatcccc aacactgcag cgcacgctgt cgctgctgca
    29581 gctgacttag gcgtgtggat ggtgaatgtt catgcctccg gtggggcgcg tatgatgacc
    29641 gcagcgcgtg aggcactggt tccgtttggc aaagatgcac cgcttttgat tgctgtgaca
    29701 gtgttgacca gcatggaagc cagcgacctg gccgatcttg gcgtgacact gtcacctgca
    29761 gattatgcag aacgtctggc agcactgacg caaaaatgtg accttgatgg tgtggtgtgt
    29821 tctgctcagg aagctgtgcg ctttaaacag gtattcggtc aggagttcaa actggttaca
    29881 ccgggcattc gtccgcaggg gagtgaagct ggtgaccagc gccgcattat gacgccagaa
    29941 caggcgttgg cggctggtgt tgattatatg gtgattggtc gcccggtaac gcaatcggta
    30001 gatccagcgc agacactgaa agcgatcaac gcctctttac agcggagtgc atgatgagtg
    30061 attccaacag ccgtctggtc tactcaacgg aaaccgggcg tattgatgag cccaaagcgg
    30121 ccccagtacg ccctaaaggc gatggagcgg tgcgtattca gcgtcagacc agtggacgta
    30181 aaggtaaggg cgtttgcctg attaccggta tcgatctcga tgatgccgaa ctgacaaaac
    30241 ttgcagcgga actgaagaaa aaatgcggct gcggcggagc agttaaagat ggagttattg
    30301 aaatccaggg cgataagcgt gatttattaa aatcactact tgaagcgaaa gggatgaagg
    30361 taaaactcgc aggcggttaa cataaaaagc cacggatata tccgtggctt tcgaatattt
    30421 tactgtgtgt attattcagt tatttcgtac tgaacagaat caagcgtatt ataattgcga
    30481 gtcgataatc gacatttatt tacgattatt taccgacctg gtgaccaata acaccaccga
    30541 cagctgcacc acctaatgta cccaacgtac tgccatcggt cagtactgca ccgcctaatg
    30601 cccctgcacc cgcgccgatt gcggtgttgc ggtcccgttt agaccagtta gaacaggcac
    30661 tcagagacat tgccaaagta attgccagaa cagccgcggt catttttttg ctcgttacaa
    30721 acataatact ctctcctgaa tttatgattc acggaagtaa gctctctata actataatag
    30781 ctaagaataa gcctggtgaa attattccgt gaaatctgct cgcgcgcagg gttatatcac
    30841 gcaggtgata attacttcct gttatatcgc tgataataat tttatatctt gagagtgtta
    30901 ataacaggta aatagtctta attatcaacc aggaatcatc ttagagcgga tgatttgcca
    30961 aactgcaaat catccgtaga gaagggaaat ggttaaatgt caacgacgtg aacggtgagt
    31021 ttactgtgct caagatgcat cagatcgctg gcgcgtattt tcgaatcggt aatcacgcga
    31081 ttgaatcgct caacgggacc gatggagtat gaatgtacag caccaaattt cgagctgtca
    31141 gtcaggacta tcgcttcgca ctctttttcc agcacggcat tgaccacatc ggtgcgcatc
    31201 atatcgcgac cggtgaatcc agtttcaggt tgccagccat caataccaat aaatgctttg
    31261 ctgaaatgta cctgctggat gcactggcgt gtcaaagggc caaccatact ttcgcttttt
    31321 ttctggtaca cgccaccgag caaaatgact tcacaaggcg cgtctttcag caaatgcgcg
    31381 atgtagctgc tgaccgtgat gatagtgaca tttttcttct gctcccccag agtccgggcg
    31441 agcagggcat tgctgctgcc attttcgata aagatggttt cgcccggttg aaccagtgac
    31501 gcggcaaact cggcgagttc acgcttcagc gtatagttgc tcatcatacg ggtttcgacg
    31561 tcatcactat caagcgaaac tgcaaagcca tgtgcacggc ggaggtaact cagtttttcg
    31621 agggtgttga gatcctggcg aatggtaact tcagaaactc cagtggcttt tgccagatcg
    31681 gttacgctaa cctgaccctg gtcaatgacc atctgtagaa tagtttgttg tcgggaattc
    31741 atcgctgtaa ttcttataac gttataatac taaataaaaa aatactgacg cgccgggcat
    31801 cctcaaatag gaaaacctga aggcacgatt gttaaatctc ccacggtgtc ttcgggatgt
    31861 ctggcgtaga ttcagcaacc tggcgcaaga gttctgcttt aagtatctcg agattatgga
    31921 tcgcagagtg gtaatcgcct gccacaagga tatctaacac aatatcaata cgttcggcaa
    31981 cgcgctgggc atcaaattca gacatcaagg acatccttat agcaaaaggg ggaaaatgaa
    32041 taatgcaaaa attgccgcta aaagagaagt gtttaacagc aacggctaat tatcatccag
    32101 gaatacgata tataatgacg ggatatagcg ctaagtatat atattcatct acttatgcgc
    32161 gcttcagata gcgtttatac cagcgttcga aggcgacggc gggcatcggt ttggcaaaca
    32221 aaaatccttg ccgctcattg atcccgttct tggttaaaaa agcatcttcc tttgcactct
    32281 ctacaccttc ggcgatcacc tgaagattca atgcctgggc cacagcgacg atcgcccgga
    32341 ccagtgactg cgagacaggt tgtttgtgaa tatctcgaac aaaaacctgg tcaagtttga
    32401 tggcatcgat cggaaagcgc gccagttgcg aaagtgaaga gtagccggta ccaaaatcat
    32461 ccagatgcac ttgcgcacct agttggctaa attgttgaat aacagacagt gccagttcat
    32521 cattctcaat cagacaactc tctgtcagtt caacatctat agggcagtat tcaaaattga
    32581 gttcctggag aacctgtttc agggcggtga aaatggtttg atcggcgagc tgacgtgcag
    32641 aaatatttac cgccactcgc aggtttatgc ctttatcccg ccactttgcc acttggcgta
    32701 cgacatcgag aatcacccaa cggcctaaag gcacaattag ccctgactct tcggcgtagg
    32761 aaatgaagtc caacggtgga atcaacccac gttcaggtga ctgccaacgt actagtgctt
    32821 ccagactgcg cacttcgaca cgctaggtga ttttcgataa ccaactgatc gttttccagt
    32881 gctttacgca agttggtatc cagccagaga tattcaaata cccgctgatt catttctgga
    32941 gtaaaaacgc aaaattgtcc tcgtccgcct tccttcgctg tgtacattgc tgtgtcggcg
    33001 tgacgaataa tagtcgcgct gtccgaacca tgttcgggag agagcgaaat acctactgaa
    33061 cagctggtat aaacttcaat taaaccaata cgaaagggga ggcgtaagcg ggtcaaaatt
    33121 cgtgatgcca ttgcttccag cgcgctttgt gaggtattgg atgccagtac cagaaactca
    33181 tcgccacctg gacgcgccaa cacctggtca tgttcgagac agcttaaaat agccaatgac
    33241 acgtcgcgta ataactggtc
//
LOCUS       DAJTUM010000039        33171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTUM010000039 DAJTUM010000000
VERSION     DAJTUM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(2..94)
                     /locus_tag="OO471_001961"
     tRNA            complement(2..94)
                     /locus_tag="OO471_001961"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO471_001962"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO471_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6243243.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO471_001963"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO471_001963"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6243244.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO471_001964"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO471_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6243245.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO471_001965"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO471_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6243246.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO471_001966"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO471_001966"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6243247.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO471_001967"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO471_001967"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6243248.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO471_001968"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO471_001968"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO471_001969"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO471_001969"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6243249.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO471_001970"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO471_001970"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6243250.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO471_001971"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO471_001971"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6243251.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO471_001972"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO471_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6243252.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO471_001973"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO471_001973"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6243253.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO471_001974"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO471_001974"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6243254.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO471_001975"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO471_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6243255.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO471_001976"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO471_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6243256.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO471_001977"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO471_001977"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6243257.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO471_001978"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO471_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6243258.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO471_001979"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO471_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6243259.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO471_001980"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO471_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6243260.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO471_001981"
     CDS             complement(20900..21019)
                     /locus_tag="OO471_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6243261.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO471_001982"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO471_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6243262.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO471_001983"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO471_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6243263.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25523)
                     /locus_tag="OO471_001984"
     CDS             complement(23682..25523)
                     /locus_tag="OO471_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6243264.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHL
                     LNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNG
                     FAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRT
                     KEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMI
                     TLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFA
                     PVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25560..26171)
                     /gene="hycB"
                     /locus_tag="OO471_001985"
     CDS             complement(25560..26171)
                     /gene="hycB"
                     /locus_tag="OO471_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6243265.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26296..26757)
                     /gene="hycA"
                     /locus_tag="OO471_001986"
     CDS             complement(26296..26757)
                     /gene="hycA"
                     /locus_tag="OO471_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6243266.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26969..27319
                     /gene="hypA"
                     /locus_tag="OO471_001987"
     CDS             26969..27319
                     /gene="hypA"
                     /locus_tag="OO471_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6243267.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27323..28195
                     /gene="hypB"
                     /locus_tag="OO471_001988"
     CDS             27323..28195
                     /gene="hypB"
                     /locus_tag="OO471_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6243268.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28186..28458
                     /gene="hypC"
                     /locus_tag="OO471_001989"
     CDS             28186..28458
                     /gene="hypC"
                     /locus_tag="OO471_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6243269.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28458..29579
                     /gene="hypD"
                     /locus_tag="OO471_001990"
     CDS             28458..29579
                     /gene="hypD"
                     /locus_tag="OO471_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6243270.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29576..30586
                     /gene="hypE"
                     /locus_tag="OO471_001991"
     CDS             29576..30586
                     /gene="hypE"
                     /locus_tag="OO471_001991"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6243271.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30660..32738
                     /gene="flhA"
                     /locus_tag="OO471_001992"
     CDS             30660..32738
                     /gene="flhA"
                     /locus_tag="OO471_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6243272.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32775..33128)
                     /locus_tag="OO471_001993"
     CDS             complement(32775..33128)
                     /locus_tag="OO471_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6243273.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7814 a         9257 c         8630 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc accactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag ccccagcccc
    24661 agccccagca ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg
    24721 ttgtgttcca tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc
    24781 agcgcgatcc cccaccacag cggtgcatta ccgcccagca gtgacagggt taacatgccc
    24841 agcaggccaa ttttcatgac taccgtagaa aacaacgcgg cagctggcgc agaggcgtta
    24901 gcatgtgcct gcggcaccca gccgtgcagc ggaataatcc cagccagcag gccaaagcca
    24961 atcacaccga gcagccagat atcggaaccg agcggcagct gttgcatacg catatccagc
    25021 aggcgcagat ccagcgtgcc ataacgctgc cacagcagcc agcaagcaat caccagcagc
    25081 agagtgccaa gacgcctcag cgcaaaccac agtttgccct ctttgctgtt gctggtgagg
    25141 aacaccgcac acaaggccat gacttcggcc attaccacga acatgccgag gttgctggcg
    25201 atgacggcgc agacggcggc agccatcaac atattgatct gcaagccgtt gcacttcacc
    25261 tgcgcgtggc gatgccagtc aatgttatag aggctgacaa acagaccgca aaggccgagg
    25321 gtaatcagcc aaatcgcgtt aagcggggag atttgcacat cgtagcttac cagcgacagc
    25381 gcaccgctca cgccaaccgc gccagtcagt acagtgaagc ccgcggctgc cgtatacaga
    25441 ctaccaaccg cgccgccaat tccggctatc cagccactta gcgctttttg aaaagagaaa
    25501 tgccagaaca gcggcggcga caaaccatgc cacgccgcta ttgatcaggg aaattgcgct
    25561 catttagcct ctccactttg agcctgctga aacaaggtga gatcgccaaa gtccgtgtta
    25621 aaggtcagct cacgcttacg tttgctgacg cgggcgatat cggtgttatc caccagatgc
    25681 agggctttag tcgggcacat ccgcacacag gccggacctt gttcatcaaa gctacaaagg
    25741 tcacatttga cggcgatcgc gcgaatacct ggcacccagt caagcaatgt gctgacacgc
    25801 gccggagcag gcggtgccgg tggcgctttc ggggtattgg cgtttgccgg aatatccagc
    25861 ggacggctgc cggaaaattc aattgcgcca aacgggcagg cgatgccgca cagtttgcag
    25921 cttacgcaca gactttcatt caactgcacg gccccatcaa cgcgggtgat ggcgttaacc
    25981 gggcagacca ccgcgcaggg tgcatcttca cagtggtgac agagctgcgg cgcagattct
    26041 ttttcattga gcatcactct caggcgcggc attgattgca ggccgtgctg gcgatgcgtc
    26101 tctgaacagg cggcctcaca ggtgtggcag ccgatacaga gcgtggagtc ggcaattaca
    26161 aaacgattca ccaggcattc ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa
    26221 atgtcatttt cgacactcat cgacacgccc atccccaaac aggcgtaacg cctgcaaaac
    26281 gggcaaagcc tcagctcatg ctgccgggct ttgtcccttt accagttggc ttaaattcac
    26341 cggcacattg ttttcaatgg cggtgtataa gctgtcgtaa acgccagcga ttttttcgag
    26401 atagtgttcc agaacctgtt cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc
    26461 aatgctcagt tgcgcctgcg caatccgctg tttgtcttcg ccatcttttg tttcgacgta
    26521 atactcgatg gtgtggctgt taaagccatc cgccagggtg acgtaaatac gaaaatgttc
    26581 aaaaaggacg aaacagagtt ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc
    26641 tttcgataac gtgatcccct gaaccagcga attgcagtag atgtgccact ggtcctgtag
    26701 gcgacgatgc cgctgtgcga tgtaatcggc tttctcgctt atttcccaaa tagtcattgt
    26761 caggttactc gtttaacaga gatgccagct ttaagcattt tttgtgccaa cttttaattt
    26821 attgttatta aagagatttt taagctaaag atgaatttcg tcgccgtgtc gacgtgtcat
    26881 ttcgacatca tcgacattat tcaccgcagg gataatcaac actggcgcaa ttattgcttg
    26941 tagctggcaa tagttaatgg gaggcgatat gcacgaaata accctctgcc aacgggcact
    27001 ggaattgatc gaacagcagg ccgcaaaaca cggcgcaaaa cgcgtaactg gggtctggct
    27061 caaaattggc gcattttctt gtgtcgaaac cagctctctt gccttttgtt ttgatctggt
    27121 ttgccgcggc agcgtggcgg aaggttgtaa actgcacctc gaagaacaag aggccgaatg
    27181 ctggtgtgaa acatgccaac agtatgtgac gctactgacc cagcgcgtcc gccgctgtcc
    27241 acagtgtcat ggtgacatgc tacagattgt ggcagacgac ggtttacaga ttcggcggat
    27301 agaaatagac caggagtgag cgatgtgtac aacatgcggt tgcggtgaag gcaacctgta
    27361 tatcgagggt gatgaacata accctcattc cgcgtttcgt agcgcgccat ttgccccggc
    27421 ggcacgcccg cagatgaaaa tcaccggcat taaagcgcct gaatttaccc ccagccagac
    27481 tgaagaaggc gacctgcatt acggtcatgg cgaagcgggc actcacacac cgggcatgag
    27541 ccagcgtcgg atgctggaag tcgaaattga cgtgctggac aaaaacaacc gtctggctga
    27601 acgcaaccgc gcgcgctttg ctgcccgcaa gcaactggtg ctcaacctgg tttccagccc
    27661 tggttccggt aaaaccaccc tgctgacgga aaccttaatg cgcctgaaag acagcgttcc
    27721 gtgcgcggtt attgaaggcg accagcaaac cgtgaacgat gccgcacgca ttcgcgctac
    27781 cggtacacca gcgattcagg tgaacaccgg taaaggctgc catcttgacg cacagatgat
    27841 tgccgacgcc gcaccgcgtc tgccactgga cgataacggt attctgttta tcgaaaacgt
    27901 tggcaacctc gtatgcccgg ccagcttcga tctcggtgaa aaacacaaag tagcggtgct
    27961 ttccgttacc gaaggtgaag acaaaccgct gaaatatccg catatgtttg ccgccgcctc
    28021 gctgatgctg ctcaacaaag ttgacctgtt gccgtatctc aactttgacg ttgagaagtg
    28081 catcgcctgc gcccgcgaag tcaatccaga aattgaaatc atccttattt ccgccaccag
    28141 cggcgaaggg atggaccagt ggctgaactg gctggagaca cagcgatgtg cataggcgtt
    28201 cccggccaga tccgcaccat tgacggcaac caggcgaaag tcgacgtctg cggcattcag
    28261 cgcgatgtcg atttaacgtt agtcggcagc tgcgatgaaa acggtcagcc gcgcgtgggc
    28321 cagtgggtac tggttcacgt tggctttgcc atgagcgtaa ttaatgaagc cgaagcacgc
    28381 gacactctcg acgccttaca aaacatgttt gacgttgagc cggatgtcgg cgcgctgttg
    28441 tatggcgagg aaaaataatg cgttttgttg atgaatatcg cgcgccggaa caggtgatgc
    28501 agttaattga gcatctgcgc gaacgtgctt cacatctctc ttacaccgcc gaacgccctc
    28561 tgcggattat ggaagtgtgt ggcggtcata cccacgccat ttttaaattc ggcctcgacc
    28621 agttactgcc tgaaaacgtt gagtttatcc acggtccggg ttgcccggtg tgcgtactgc
    28681 cgatgggtag aatcgacacc tgcgtggaga ttgccagcca tccggaagtc atcttctgta
    28741 cctttggcga cgccatgcgc gtaccaggga aacagggatc gctgttgcag gcaaaagcac
    28801 gcggtgccga tgtgcgcatc gtttactcgc cgatggatgc gttgaaactg gcgcaggaga
    28861 atccaacccg caaagtggtg ttcttcggct taggttttga aaccaccatg ccgaccaccg
    28921 ccatcactct gcaacaggcg aaagcgcgtg atgtgcagaa tttttacttc ttctgccagc
    28981 acattacgct tatcccgacg ctgcgcagtt tgctggaaca gccggataac ggtatcgacg
    29041 cgttcctcgc gccgggccac gttagtatgg ttatcggcac tgatgcctat aattttatcg
    29101 ccagcgattt tcatcgtccg ctggtggtcg ctggtttcga accccttgat ctactgcaag
    29161 gcgtggtcat gctggtggag cagaaaatag cggcccacag caaggtagag aatcagtatc
    29221 gtcgggtggt accggatgcc ggtaacctac tggcgcaaca ggcgattgcc gatgtgttct
    29281 gtgtcaacgg cgacagcgaa tggcgcggct taggcgtgat tgaatcttct ggcgtgcacc
    29341 tgacgccgga ttatcaacga ttcgatgccg aagcacattt ccgcccggca ccgcagcagg
    29401 tctgcgatga cccgcgcgcg cgttgtggtg aggtattaac gggcaaatgt aagccgcatc
    29461 aatgcccgct gtttggtaac acctgtaatc ctcaaaccgc atttggtgca ctgatggtct
    29521 cctccgaagg agcgtgcgcc gcgtggtatc agtatcgtca gcaggagagt gaagcgtgaa
    29581 taatatccaa ctcgcccacg gcagcggcgg ccaggcgatg cagcaattaa tcaacagcct
    29641 gtttatggaa gcctttgcca acccgtggct ggcagagcag gaagatcagg cacgtcttga
    29701 tctggcgcag ctggtagcgg aaggcgaccg tctggcgttc tccaccgaca gttacgtcat
    29761 tgacccgctg ttctttcctg gcggtaatat cggcaagctg gcgatttgcg gcaccgcgaa
    29821 tgacgttgcg ggcagtggcg ctattccgcg ctatctctcc tgtggcttta tcctcgaaga
    29881 aggattgccg atggagacac tgaaagccgt agtgaccagc atggcagaaa ccgcccgcac
    29941 ggcaggcatt gccatcgtta ctggcgatac taaagtggtg cagcgcggcg cggcagataa
    30001 actgtttatc aacaccgctg gcatgggcgc aattccggcg aatattcact ggggcgcaca
    30061 gacgctaacc gcaggcgatg tattgctggt tagcggtaca ctcggcgacc acggggcgac
    30121 tatccttaac ctgcgtgagc agctggggct ggatggcgaa ctggtcagcg actgcgcggt
    30181 gctgacgccg cttattcaga cgctgcgtga cattcccggc gtgaaagcgc tgcgtgatgc
    30241 cacccgtggt ggtgtaaacg cggtggttca tgagttcgcg gcagcctgcg gttgtggtat
    30301 tgaaatttct gaatcagcgc tgccggttaa acctgccgtg cgcggcgttt gcgaattgct
    30361 gggactggac gccctgaact ttgccaacga aggcaaactg gtgatcgccg ttgaacgcaa
    30421 cgcggcagag caagtgctgg cagcgttaca ttcccatcca ctggggaaag acgcggcgct
    30481 gattggtgaa gtggtggaac gtaaaggtgt tcgtcttgcc ggtctgtatg gcgtgaaacg
    30541 aaccctcgat ttaccacacg ccgaaccgct tccgcgtata tgctaataaa attctaaatc
    30601 tcctatagtt agtcaatgac cttttgcacc gctttgcggt gctttcctgg aagaacaaaa
    30661 tgtcatatac accgatgagt gatctcggac agcaagggtt gttcgacatc actcggacac
    30721 tattgcagca gcccgatctg gcctcgctgt gtgaggctct ttcgcaactg gtaaagcgtt
    30781 ctgcgctcgc cgacaacgcg gctattgtgt tgtggcaagc gcagactcaa cgtgcgtctt
    30841 attacgcgtc gcgtgaaaaa gacaccccca ttaaatatga agacgaaact gttctggcac
    30901 acggtccggt acgcagcatt ttgtcgcgcc ctgatacgct gcattgcagt tacgaagaat
    30961 tttgtgaaac ctggccgcag ctggccgcag gtgggctata ccccaaattt ggtcactatt
    31021 gcctgatgcc actggcggcg ggagggcata tttttggtgg ctgtgaattt attcgttatg
    31081 acgagcgtcc ctggagcgaa aaagagttca atcgtctgca aacatttacg cagatcgttt
    31141 ctgtcgtcac cgaacaaatc cagagccgcg tcgtcaacaa tgtcgactat gagttgttat
    31201 gccgggagcg cgataacttc cgcatcctgg ttgccattac caacgcggtg ctttcccgcc
    31261 tggatatgga cgaactggtc agcgaagtcg ccaaagaaat ccattactat ttcgacattg
    31321 acgatatcag cattgtctta cgcagccacc gtaaaaacaa actcaacatc tactccactc
    31381 actatcttga taaacaacat cccgcccacg aacagagcga agtcgatgaa gccggaaccc
    31441 tcaccgaacg cgtgttcaaa agtaaagaga tgctgctgat caatctctac gagcgggacg
    31501 atttagcccc ctatgaacgc atgttgttcg acacctgggg caaccagatt caaaccttgt
    31561 gcctgttacc gctgatgtct ggcgacacca tgctgggcgt gctgaaactg gcgcaatgcg
    31621 aagagaaagt gtttaccact accaatctga atttgctgcg ccagattgcc gaacgtgtgg
    31681 caatcgctgt cgataacgcc ctcgcctatc aggaaatcca tcgtctgaaa gaacggctgg
    31741 ttgatgaaaa cctcgccctg accgagcagc tcaacaatgt tgatagtgaa tttggcgaga
    31801 ttattggccg cagcgaagcc atgtacagcg tgcttaaaca agttgaaatg gtggcgcaaa
    31861 gtgacagtac cgtgctgatc ctcggtgaaa ctggctcggg taaagagctg attgcccgtg
    31921 cgatccataa tctcagtggg cgtaataatc gccgcatggt caaaatgaac tgcgcggcga
    31981 tgcctgccgg attgctggaa agcgatctgt ttggtcatga gcgtggggct tttaccggtg
    32041 ccaacgccca gcgcattggt cgttttgaac tggcggataa aagctctctg ttcctcgacg
    32101 aagtgggcga tatgccactg gagttacagc cgaagttgct gcgtgtattg caggaacagg
    32161 agtttgaacg tctcggcagc aacaaaatca ttcagacgga cgtacgttta attgccgcga
    32221 ctaaccgcga tctgaaaaaa atggtcgccg accgagagtt ccgtagcgat ctctattacc
    32281 gcctgaacgt attcccgatt cacctgccgc cactacgcga gcgtccggaa gatattccgc
    32341 tgctggcgaa agcctttacc ttcaaaattg cccgtcgtct ggggcgcaat atcgacagca
    32401 ttcctgccga gacgctgcgc accttgagca acatggagtg gccgggtaac gtacgcgaac
    32461 tggaaaacgt cattgagcgc gcagtattgc taacacgcgg caacgtgctg cagctgtcat
    32521 tgccagatat tgctttaccg gagcctgaaa cgccgcctgc cgcaacggtt gtcgcccagg
    32581 agggcgaaga tgaatatcag ttgattgtgc gcgtgctgaa agaaaccaac agcgtggttg
    32641 ccgggcctaa aggcgctgcg caacgtctgg ggctgaaacg cacgaccctg ctgtcacgga
    32701 tgaagcggct gggaattgat aaatcggcat tgatttaact gcaaattgcc ggacagatct
    32761 gcctgtccgg catactattc acgaggtttt ttcggacgat atttttccgg cagttctggc
    32821 accggacgct tgtcatcgat gagatgacgc acggttaaga tcggatgacg ccacagcatt
    32881 ctcggcccgg cccaacgcat aatctgtttc atctcttcac gctttgcagg ctggtaacag
    32941 tgcaccggac actgcttaca ggctggtttc tcttcgccga acacacattt atccagccgc
    33001 ttttgcgcgt aaacaaacaa cgcctcgtaa tgctccggct ccgctgacgc ctgcgggcat
    33061 ttcgcttgat aaagatcgat cattttttta atcgtcagtt tttcacgaga gatacgcttg
    33121 ccggacatgc tgcctccacc tcattaagat gcatttatat tacatcttaa t
//
LOCUS       DAJTUM010000040        31175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTUM010000040 DAJTUM010000000
VERSION     DAJTUM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            379..2037
                     /locus_tag="OO471_001994"
     CDS             379..2037
                     /locus_tag="OO471_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6243274.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2034..2990)
                     /locus_tag="OO471_001995"
     CDS             complement(2034..2990)
                     /locus_tag="OO471_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6243275.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3141..4256
                     /gene="macA"
                     /locus_tag="OO471_001996"
     CDS             3141..4256
                     /gene="macA"
                     /locus_tag="OO471_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6243276.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4253..6199
                     /gene="macB"
                     /locus_tag="OO471_001997"
     CDS             4253..6199
                     /gene="macB"
                     /locus_tag="OO471_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6243277.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6272..6496)
                     /gene="cspD"
                     /locus_tag="OO471_001998"
     CDS             complement(6272..6496)
                     /gene="cspD"
                     /locus_tag="OO471_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6243278.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6819..7139
                     /gene="clpS"
                     /locus_tag="OO471_001999"
     CDS             6819..7139
                     /gene="clpS"
                     /locus_tag="OO471_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6243279.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7170..9446
                     /gene="clpA"
                     /locus_tag="OO471_002000"
     CDS             7170..9446
                     /gene="clpA"
                     /locus_tag="OO471_002000"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6243280.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9849..9936)
                     /locus_tag="OO471_002001"
     tRNA            complement(9849..9936)
                     /locus_tag="OO471_002001"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9900..9902),aa:Ser,seq:gga)
     gene            complement(10190..10408)
                     /gene="infA"
                     /locus_tag="OO471_002002"
     CDS             complement(10190..10408)
                     /gene="infA"
                     /locus_tag="OO471_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6243281.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10693..11397)
                     /gene="aat"
                     /locus_tag="OO471_002003"
     CDS             complement(10693..11397)
                     /gene="aat"
                     /locus_tag="OO471_002003"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6243282.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11439..13160)
                     /gene="cydC"
                     /locus_tag="OO471_002004"
     CDS             complement(11439..13160)
                     /gene="cydC"
                     /locus_tag="OO471_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6243283.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13161..14927)
                     /gene="cydD"
                     /locus_tag="OO471_002005"
     CDS             complement(13161..14927)
                     /gene="cydD"
                     /locus_tag="OO471_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6243284.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15050..16015)
                     /gene="trxB"
                     /locus_tag="OO471_002006"
     CDS             complement(15050..16015)
                     /gene="trxB"
                     /locus_tag="OO471_002006"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6243285.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16559..17053
                     /gene="lrp"
                     /locus_tag="OO471_002007"
     CDS             16559..17053
                     /gene="lrp"
                     /locus_tag="OO471_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6243286.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17188..21216
                     /gene="ftsK"
                     /locus_tag="OO471_002008"
     CDS             17188..21216
                     /gene="ftsK"
                     /locus_tag="OO471_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6243287.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21371..21982
                     /gene="lolA"
                     /locus_tag="OO471_002009"
     CDS             21371..21982
                     /gene="lolA"
                     /locus_tag="OO471_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6243288.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21993..23336
                     /gene="rarA"
                     /locus_tag="OO471_002010"
                     /gene_synonym="mgsA"
     CDS             21993..23336
                     /gene="rarA"
                     /locus_tag="OO471_002010"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6243289.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23427..24719
                     /gene="serS"
                     /locus_tag="OO471_002011"
     CDS             23427..24719
                     /gene="serS"
                     /locus_tag="OO471_002011"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6243290.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24959..27402
                     /gene="dmsA"
                     /locus_tag="OO471_002012"
                     /pseudo
     CDS             24959..27402
                     /gene="dmsA"
                     /locus_tag="OO471_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27413..28030
                     /gene="dmsB"
                     /locus_tag="OO471_002013"
     CDS             27413..28030
                     /gene="dmsB"
                     /locus_tag="OO471_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6243291.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28032..28823
                     /locus_tag="OO471_002014"
     CDS             28032..28823
                     /locus_tag="OO471_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6243292.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28859..29485)
                     /locus_tag="OO471_002015"
     CDS             complement(28859..29485)
                     /locus_tag="OO471_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6243293.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29800..30948
                     /locus_tag="OO471_002016"
     CDS             29800..30948
                     /locus_tag="OO471_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243294.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7643 c         8370 g         7473 t
ORIGIN      
        1 atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa aaacgaaggc
       61 gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag cgggttgccc
      121 catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat cctttaggcg
      181 taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg ggcttatcag
      241 caagagggag tataacgcga ttattcgctc atttttcaga caattgccat gcttaaatgt
      301 gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa ggataagtca
      361 catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt tcgcggtatc
      421 aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa cgcgtggggt
      481 aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga tctctaccat
      541 tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga acatcatctg
      601 catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg ccgttatcgg
      661 ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt ttatcgtctg
      721 gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct cgataaagac
      781 ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt gcgtttaatg
      841 ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg cacggtgcca
      901 aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg tgagttgtcc
      961 tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc aatggtgcag
     1021 ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg tttaatgcgg
     1081 cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa ccggatgatt
     1141 gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc tactttattg
     1201 caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct aattgaagat
     1261 ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt gaatcttttg
     1321 cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac tccagtggaa
     1381 catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct ggggccaagt
     1441 ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa tcgtccgtca
     1501 tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg ggttatcaat
     1561 gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt cattgagttt
     1621 gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat tgaatggcat
     1681 gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg cagcctgttg
     1741 aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct ggatatggaa
     1801 cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca aatcccggaa
     1861 aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc ttccaaaccc
     1921 gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc cgtaccaacg
     1981 ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc ggattaaccg
     2041 cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg ccgacggtat
     2101 tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg tgctggaagg
     2161 cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc gttgtaatca
     2221 gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg cgtttcattg
     2281 ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc cgcttccatc
     2341 acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat ttcctgatgt
     2401 ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt cccctgatat
     2461 tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg gaacaggatt
     2521 gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt gaactgctca
     2581 ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata taagctgaat
     2641 tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc ctccagcaat
     2701 tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg cagtcgcggc
     2761 tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat ccactcaacg
     2821 cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact acagcgatgc
     2881 cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga caggctgctg
     2941 agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat gaaatcaatg
     3001 gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat agtgtgatta
     3061 ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc ggattggcat
     3121 atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt tacgttattg
     3181 cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac gcacccgtgc
     3241 cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg ctggcgaccg
     3301 gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt cagttgaaaa
     3361 cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg gtcattgatc
     3421 ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag ctacgcgcgc
     3481 agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc cgtcagcaac
     3541 gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg acggagatgg
     3601 ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat caggcctctc
     3661 tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg gctggggaag
     3721 tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa gcaccgaaca
     3781 ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct gaagcggatg
     3841 taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat ccactgacgc
     3901 gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac gacgctattt
     3961 tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg gatatgaccg
     4021 cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct ctgtcggcgt
     4081 taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat ggtgaaacac
     4141 gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt gtcaaagggc
     4201 tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca caatgacgcc
     4261 tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc aggttgaggt
     4321 gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg ttggcgcttc
     4381 gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg ccaccagcgg
     4441 cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc tggcgcaact
     4501 gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc atttaaccgt
     4561 cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac agcgactgct
     4621 tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt attatccggc
     4681 acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga tgaacggcgg
     4741 tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg gcgaagaggt
     4801 gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg tcacccacga
     4861 tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg aaattgtgcg
     4921 caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg ttgtcaatac
     4981 agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga tggcatggcg
     5041 ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta ttatcggtat
     5101 tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg tgctggcgga
     5161 tattcgttct attggtacga atactattga tgtctatccc gggaaagatt ttggcgatga
     5221 cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc aaaaacaacc
     5281 gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt ataacaatgt
     5341 tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct atggcatgac
     5401 cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc aggtcgtggt
     5461 tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg ttggcgaggt
     5521 gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa aacagtcgat
     5581 gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt ccgggcgagt
     5641 tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat ttgacagcgc
     5701 cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga aggatttctt
     5761 tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta ctttacaact
     5821 gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg taatgaatat
     5881 tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg ctgtaggtgc
     5941 gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt gcctggtcgg
     6001 tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc ttttcttacc
     6061 cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct gctcgacggc
     6121 caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg atccagtaga
     6181 tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc tggcattttg
     6241 cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct acttcgacgg
     6301 gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac tgaacggatt
     6361 gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga gcgaaaatat
     6421 cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg aaccacttaa
     6481 cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa gatggaataa
     6541 accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac aatttagaga
     6601 attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa tcaaaaattt
     6661 gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa cgatagatga
     6721 tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca gcggcagggc
     6781 gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg aacgactggc
     6841 tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg ccatctatgt
     6901 ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt gacgtgttac
     6961 aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct gttcactacc
     7021 aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa gtggcgatgg
     7081 tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa aaagcctgaa
     7141 tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa ctcagtttaa
     7201 atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc gagcacttgt
     7261 tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt tctgtggatt
     7321 tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc gttctgcctg
     7381 ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta ctgcaacgtg
     7441 cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac gttctggtcg
     7501 ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat gaagtcagcc
     7561 gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg acacagtctt
     7621 ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa cgtatggaga
     7681 atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca ctaattggtc
     7741 gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa aacaacccgc
     7801 tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt gcctggcgaa
     7861 ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct ctcgatatcg
     7921 gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt aaagcgttgc
     7981 tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc cacaccatta
     8041 tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc aaaccgttgc
     8101 tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc agcaacattt
     8161 tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact gaaccgtcga
     8221 tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg caccacgacg
     8281 tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa tacattaacg
     8341 atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct cgcgcacgtc
     8401 tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag tccgtggtgg
     8461 cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat accctgaaaa
     8521 aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt gaggcgctga
     8581 ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg gtcggttcgt
     8641 tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag ctttcgaaag
     8701 cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc cataccgtca
     8761 gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt ttgctgaccg
     8821 atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag aaagcgcatc
     8881 cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc gataacaacg
     8941 gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc ggggtacggg
     9001 aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat gcgatggaag
     9061 agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt atctggttcg
     9121 atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc gagttgcagg
     9181 ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt aactggctgg
     9241 ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc atccaggaca
     9301 acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac ggcggtcagg
     9361 tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag agtgcacaaa
     9421 agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga agtccgtaat
     9481 ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc ctatttttgg
     9541 aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa aaagggtgct
     9601 gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag tatttgagtt
     9661 cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt
     9721 cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc ccaccatgtt
     9781 cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc
     9841 tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt
     9901 ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta ccaaacctca
     9961 tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga agaattttaa
    10021 tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac atcgagacaa
    10081 gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa ggccggttaa
    10141 accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac
    10201 ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc
    10261 ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt
    10321 gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga acggtacctt
    10381 gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta ccgtaatttg
    10441 aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa
    10501 caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg
    10561 tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt ccttatcggc
    10621 agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg gggagaaatg
    10681 tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga aattattcgg
    10741 caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg gaatttcgca
    10801 ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct taccgccatg
    10861 accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt ccatccggct
    10921 gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac cgccgacaag
    10981 ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa ggcgatggta
    11041 ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg cacagccttc
    11101 aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg
    11161 cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg gatcgggcga
    11221 ccaccagagg atggggtcgt ctggagaaaa ccacggaaaa ataccacgct ggtaagccat
    11281 taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag gctcacgtaa
    11341 tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca agcgcatgac
    11401 cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg cttgaactgg
    11461 taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat aatttgcccg
    11521 ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt
    11581 aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct ttcggttgtg
    11641 gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg taacagcgta
    11701 cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc ttcacctaac
    11761 caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg caagatctcc
    11821 gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg cagtgtggcg
    11881 ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc cgcttcattc
    11941 aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc ccatgcgcgg
    12001 gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat cgctatatgt
    12061 tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg ctccggataa
    12121 gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca
    12181 gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc agaggcaatg
    12241 acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac
    12301 gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc aacgccgcca
    12361 gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc
    12421 gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat ttctgtattc
    12481 tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc
    12541 agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg
    12601 ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa cataacgctg
    12661 cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac caccataatc
    12721 accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc gagcgtatca
    12781 acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag tccggcaggg
    12841 gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc
    12901 gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat tgcggcacca
    12961 cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac ccccgcaacc
    13021 gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc gagcagcgtc
    13081 acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata cagtgccaga
    13141 tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat
    13201 gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg gccatcctgc
    13261 ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac cattaacgtt
    13321 gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact gtgagcatca
    13381 aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa cgcacgggcc
    13441 accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc aacaggtgta
    13501 tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc cagcgctgct
    13561 tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg caattttgct
    13621 gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga ttctggtgat
    13681 aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag aaaaccagaa
    13741 agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac tgcacgttgg
    13801 cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg cgacgtgata
    13861 aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc ctcaccgcgc
    13921 tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc accaacagcc
    13981 tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca
    14041 aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg accaaaatcc
    14101 agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga cagcgaggta
    14161 aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat tgtccgttgg
    14221 cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg accaaaaata
    14281 cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg agcaagagcg
    14341 agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat aaacaacgga
    14401 attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg gaagatcgcc
    14461 accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata gcgtgcatag
    14521 taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc cgcaggttta
    14581 ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg gcggatggca
    14641 aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac cacccatgcg
    14701 cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc acggggaata
    14761 ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc aatgatcaat
    14821 atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga gatgacgctt
    14881 tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc acgcttagca
    14941 ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc
    15001 catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt
    15061 cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca gtaccggccg
    15121 aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa
    15181 tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag ccgttttcca
    15241 gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca acaaacagac
    15301 cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag
    15361 tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt
    15421 tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg cggaaaccgt
    15481 cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc gcctcttcaa
    15541 ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc
    15601 aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc aggccgagat
    15661 agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc
    15721 cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca aaaatgatct
    15781 cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga ccggtcagat
    15841 cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt
    15901 ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt
    15961 atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat
    16021 ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac gtaaaaaaag
    16081 agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca ggtcaaattc
    16141 tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat taaatgatga
    16201 ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg ttttgctttg
    16261 acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat tcttatatgc
    16321 gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg tatggcgtgg
    16381 acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc accgtagacc
    16441 caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt agcgactctg
    16501 aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga caataataat
    16561 ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta acattcttaa
    16621 tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg tgggactttc
    16681 cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta ttcagggcta
    16741 tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg ttgagattac
    16801 tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac aaaaacttga
    16861 agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga aaacacgcgt
    16921 gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc tgcctggcgt
    16981 caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc gtctggttat
    17041 taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca ctcctgttaa
    17101 tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc atcgggcagg
    17161 aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga caaagaagtc
    17221 acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat ccttattgtc
    17281 ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga ccccagctgg
    17341 tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg tgcgtggttg
    17401 gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt cattattgtc
    17461 ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga ttattttgcc
    17521 gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg tctggcggca
    17581 atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag cttactaagc
    17641 actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct ctgcgtttgg
    17701 gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga aaaactcggc
    17761 ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga tgatacctgg
    17821 gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca cggcaaacag
    17881 catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa acggttggcg
    17941 gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc cggtaagcgg
    18001 atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga cccggacgac
    18061 gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga tccattatta
    18121 aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac ggcgacacaa
    18181 agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc tgttgatgtt
    18241 ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac gggagagccg
    18301 gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca gcctgcagtg
    18361 caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta tgcacctgca
    18421 gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc ggtggcaggt
    18481 aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact
    18541 gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc gcaacccgtt
    18601 gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg
    18661 ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg
    18721 gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgctg
    18781 aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg
    18841 gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca
    18901 gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg gattggcccg
    18961 cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc gtcttacggt
    19021 attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca gcgcaatcag
    19081 tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca ggatgaactg
    19141 gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgtg
    19201 cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg
    19261 caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg
    19321 gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgctg
    19381 tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc acagcagcag
    19441 tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc acaacagcag
    19501 gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca gcagcaatat
    19561 cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca acaaccggtt
    19621 gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc gcaggatacc
    19681 ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa accgacgacg
    19741 ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc
    19801 tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt ccgtattaaa
    19861 gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact taacctggct
    19921 cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg
    19981 acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta
    20041 ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc
    20101 gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt
    20161 gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct
    20221 gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt
    20281 ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcacctg
    20341 ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac
    20401 gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat
    20461 aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag
    20521 ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtcgtg
    20581 ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata
    20641 gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt
    20701 ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt
    20761 accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg
    20821 ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat
    20881 ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt
    20941 cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg tgcgggtggt
    21001 ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa
    21061 aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca
    21121 gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt
    21181 aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg gataaggcgc
    21241 ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag
    21301 ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg
    21361 aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct tagtagcaag
    21421 cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt
    21481 ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca
    21541 gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga
    21601 aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc
    21661 tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa
    21721 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac
    21781 gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac
    21841 aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc agctgaaatc
    21901 ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac
    21961 ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg
    22021 ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg
    22081 gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatt
    22141 tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga
    22201 ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag
    22261 agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc
    22321 tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata
    22381 ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta
    22441 attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt acagaggata
    22501 ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt ggtcaggata
    22561 ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc gacgcgcgcc
    22621 gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat agcggtaagc
    22681 gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc cgctttgata
    22741 acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta cgtggtagcg
    22801 cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc gatccgttat
    22861 atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc gatccacggg
    22921 cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg
    22981 aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct
    23041 acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat gacgtgccgg
    23101 ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc
    23161 gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa
    23221 tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag attggtgaaa
    23281 agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt
    23341 atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca
    23401 tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag
    23461 acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg
    23521 ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta
    23581 actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac
    23641 gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt
    23701 tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc
    23761 cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc ccgcgtgagt
    23821 ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg
    23881 cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca
    23941 tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg
    24001 agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga
    24061 aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact
    24121 atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg
    24181 atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg
    24241 gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa
    24301 tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg actggtcatg
    24361 cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg
    24421 acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca
    24481 cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg
    24541 cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg
    24601 gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc
    24661 gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat
    24721 acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct ccctttgata
    24781 ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat
    24841 ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg ctatagaaat
    24901 aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt gagccattat
    24961 gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg gtttggtaaa
    25021 aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg
    25081 gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag
    25141 cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga
    25201 aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct acactaggtt
    25261 cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga ccgcctgaaa
    25321 tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa
    25381 gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag
    25441 tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg
    25501 ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct taaccattac
    25561 ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg cggctgggca
    25621 gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt tggtaataac
    25681 ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag
    25741 aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc
    25801 gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa cggtctggcg
    25861 tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc
    25921 tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa agcttatatt
    25981 ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca aatcaccggt
    26041 gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt
    26101 atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc
    26161 tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag cggcgcgcgt
    26221 gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc
    26281 agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg
    26341 cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat
    26401 gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga aatcctccag
    26461 gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa
    26521 tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcactggat
    26581 gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt
    26641 gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa
    26701 cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg
    26761 gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag
    26821 cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac
    26881 ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc
    26941 gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc
    27001 tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac aggtttccac
    27061 tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc ggcttgccgt
    27121 caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa cggcgataaa
    27181 gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac gccacgaatg
    27241 atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc aaaacgtgtc
    27301 gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg
    27361 aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac
    27421 ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc
    27481 ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt atgaatatgc
    27541 tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct
    27601 gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc
    27661 gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg
    27721 ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat
    27781 gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga
    27841 atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg
    27901 cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat
    27961 caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa
    28021 ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg
    28081 ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc
    28141 gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta ctgatgggca
    28201 ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt aactcgctca
    28261 accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg
    28321 taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc
    28381 tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt
    28441 ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg ggctgggcga
    28501 tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg atgtcagtga
    28561 tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca gcgctggtgc
    28621 cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt
    28681 ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca
    28741 ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg
    28801 tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt
    28861 atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt
    28921 tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcaag
    28981 ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc cacgccgaat
    29041 gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca tcaaaccctt
    29101 cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa
    29161 ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt
    29221 taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctagaa
    29281 ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt aagttgaaat
    29341 acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc
    29401 gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca tcatttttat
    29461 caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctga
    29521 aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg
    29581 accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa
    29641 tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata taaatcgata
    29701 ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat
    29761 gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct
    29821 gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca
    29881 ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc
    29941 tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaaacgc
    30001 attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc
    30061 cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc
    30121 tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac
    30181 cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt tttaggccag
    30241 ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt tacaggtttg
    30301 acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac
    30361 catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat
    30421 ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc tctgtacctc
    30481 aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact ggtcagtgcg
    30541 ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg actgctggtt
    30601 ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag ccaggcggcg
    30661 atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc ggtggcgatg
    30721 gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca agccttactg
    30781 ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct aatgcagaat
    30841 ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta tttgctgatg
    30901 ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc
    30961 aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg acttgctttt
    31021 ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt gcatttcgct
    31081 atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc cctttaattt
    31141 tggcgaagga tttgtctatg gctgggaatg ttcag
//
LOCUS       DAJTUM010000041        30296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTUM010000041 DAJTUM010000000
VERSION     DAJTUM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO471_002017"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO471_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6243295.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO471_002018"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO471_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6243296.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO471_002019"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO471_002019"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6243297.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO471_002020"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO471_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO471_002021"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO471_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6243298.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO471_002022"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO471_002022"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6243299.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO471_002023"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO471_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6243300.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OO471_002024"
     CDS             7490..7639
                     /locus_tag="OO471_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243301.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OO471_002025"
     CDS             7840..9423
                     /locus_tag="OO471_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6243302.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO471_002026"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO471_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6243303.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO471_002027"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO471_002027"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6243304.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO471_002028"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO471_002028"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6243305.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="OO471_002029"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="OO471_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="OO471_002030"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="OO471_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="OO471_002031"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="OO471_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="OO471_002032"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="OO471_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6243306.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="OO471_002033"
     CDS             20753..22168
                     /locus_tag="OO471_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243307.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="OO471_002034"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="OO471_002034"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6243308.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="OO471_002035"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="OO471_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6243309.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="OO471_002036"
     CDS             24478..24810
                     /locus_tag="OO471_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6243310.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="OO471_002037"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="OO471_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6243311.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /locus_tag="OO471_002038"
     CDS             complement(26660..26977)
                     /locus_tag="OO471_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243312.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27383..28282
                     /gene="yegS"
                     /locus_tag="OO471_002039"
     CDS             27383..28282
                     /gene="yegS"
                     /locus_tag="OO471_002039"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6243313.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28364..29137)
                     /locus_tag="OO471_002040"
     CDS             complement(28364..29137)
                     /locus_tag="OO471_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6243314.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="OO471_002041"
     CDS             complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="OO471_002041"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6243315.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7013 a         8046 c         7828 g         7409 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 caaaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggtg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctattcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgagat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    27061 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
    27121 tcagggtaac tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa
    27181 tattaggtat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
    27241 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
    27301 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
    27361 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
    27421 taaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
    27481 gacgatccat gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc
    27541 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
    27601 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
    27661 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
    27721 gaaactggca attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac
    27781 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
    27841 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
    27901 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
    27961 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa
    28021 cgcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
    28081 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
    28141 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
    28201 gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
    28261 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat
    28321 gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt
    28381 ttacccgtgt cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa
    28441 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg
    28501 aatcgagaac ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac
    28561 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
    28621 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
    28681 tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    28741 acgctgccgg gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg
    28801 cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt
    28861 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    28921 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    28981 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    29041 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    29101 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    29161 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    29221 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac
    29281 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    29341 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    29401 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    29461 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    29521 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    29581 cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    29641 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    29701 agctaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    29761 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    29821 aatgaaaagc atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca
    29881 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    29941 catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac
    30001 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta
    30061 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    30121 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    30181 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg
    30241 caacgcgcac gataccatca gtatcattca ctactgattt cataaaaact cctgat
//
LOCUS       DAJTUM010000042        30064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTUM010000042 DAJTUM010000000
VERSION     DAJTUM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..70
                     /locus_tag="OO471_002042"
     CDS             <1..70
                     /locus_tag="OO471_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243316.1"
                     /translation="LRFAIREGGRTVGAGVVAKVLS"
     gene            300..683
                     /gene="secE"
                     /locus_tag="OO471_002043"
     CDS             300..683
                     /gene="secE"
                     /locus_tag="OO471_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6243317.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            685..1230
                     /gene="nusG"
                     /locus_tag="OO471_002044"
     CDS             685..1230
                     /gene="nusG"
                     /locus_tag="OO471_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6243318.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1389..1817
                     /gene="rplK"
                     /locus_tag="OO471_002045"
     CDS             1389..1817
                     /gene="rplK"
                     /locus_tag="OO471_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6243319.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1821..2525
                     /gene="rplA"
                     /locus_tag="OO471_002046"
     CDS             1821..2525
                     /gene="rplA"
                     /locus_tag="OO471_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6243320.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2938..3435
                     /gene="rplJ"
                     /locus_tag="OO471_002047"
     CDS             2938..3435
                     /gene="rplJ"
                     /locus_tag="OO471_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6243321.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3502..3867
                     /gene="rplL"
                     /locus_tag="OO471_002048"
     CDS             3502..3867
                     /gene="rplL"
                     /locus_tag="OO471_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6243322.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4187..8215
                     /gene="rpoB"
                     /locus_tag="OO471_002049"
     CDS             4187..8215
                     /gene="rpoB"
                     /locus_tag="OO471_002049"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6243323.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8292..12515
                     /gene="rpoC"
                     /locus_tag="OO471_002050"
     CDS             8292..12515
                     /gene="rpoC"
                     /locus_tag="OO471_002050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6243324.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12757..13890)
                     /gene="thiH"
                     /locus_tag="OO471_002051"
     CDS             complement(12757..13890)
                     /gene="thiH"
                     /locus_tag="OO471_002051"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6243325.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13887..14657)
                     /gene="thiG"
                     /locus_tag="OO471_002052"
     CDS             complement(13887..14657)
                     /gene="thiG"
                     /locus_tag="OO471_002052"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6243326.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14659..14859)
                     /gene="thiS"
                     /locus_tag="OO471_002053"
     CDS             complement(14659..14859)
                     /gene="thiS"
                     /locus_tag="OO471_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6243327.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14843..15598)
                     /gene="thiF"
                     /locus_tag="OO471_002054"
     CDS             complement(14843..15598)
                     /gene="thiF"
                     /locus_tag="OO471_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6243328.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15591..16226)
                     /gene="thiE"
                     /locus_tag="OO471_002055"
     CDS             complement(15591..16226)
                     /gene="thiE"
                     /locus_tag="OO471_002055"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6243329.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16226..18121)
                     /gene="thiC"
                     /locus_tag="OO471_002056"
     CDS             complement(16226..18121)
                     /gene="thiC"
                     /locus_tag="OO471_002056"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6243330.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18202..18302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18355..18831)
                     /gene="rsd"
                     /locus_tag="OO471_002057"
     CDS             complement(18355..18831)
                     /gene="rsd"
                     /locus_tag="OO471_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6243331.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18926..19699
                     /gene="nudC"
                     /locus_tag="OO471_002058"
     CDS             18926..19699
                     /gene="nudC"
                     /locus_tag="OO471_002058"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6243332.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19739..20803
                     /gene="hemE"
                     /locus_tag="OO471_002059"
     CDS             19739..20803
                     /gene="hemE"
                     /locus_tag="OO471_002059"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6243333.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20960..21631
                     /gene="nfi"
                     /locus_tag="OO471_002060"
     CDS             20960..21631
                     /gene="nfi"
                     /locus_tag="OO471_002060"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6243334.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21674..22264
                     /locus_tag="OO471_002061"
     CDS             21674..22264
                     /locus_tag="OO471_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6243335.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22451..22723
                     /gene="hupA"
                     /locus_tag="OO471_002062"
     CDS             22451..22723
                     /gene="hupA"
                     /locus_tag="OO471_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6243336.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22736..23431
                     /locus_tag="OO471_002063"
     CDS             22736..23431
                     /locus_tag="OO471_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6243337.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23433..23858)
                     /gene="zraP"
                     /locus_tag="OO471_002064"
     CDS             complement(23433..23858)
                     /gene="zraP"
                     /locus_tag="OO471_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6243338.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24096..25472
                     /gene="zraS"
                     /locus_tag="OO471_002065"
     CDS             24096..25472
                     /gene="zraS"
                     /locus_tag="OO471_002065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6243339.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25469..26794
                     /gene="zraR"
                     /locus_tag="OO471_002066"
     CDS             25469..26794
                     /gene="zraR"
                     /locus_tag="OO471_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6243340.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26791..28080)
                     /gene="purD"
                     /locus_tag="OO471_002067"
     CDS             complement(26791..28080)
                     /gene="purD"
                     /locus_tag="OO471_002067"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6243341.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28092..29681)
                     /gene="purH"
                     /locus_tag="OO471_002068"
     CDS             complement(28092..29681)
                     /gene="purH"
                     /locus_tag="OO471_002068"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6243342.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7034 a         7838 c         8274 g         6918 t
ORIGIN      
        1 tctgcgtttc gcaatccgtg aaggcggccg taccgttggc gcgggcgttg tagcaaaagt
       61 tctgagctaa ttgccgataa catttgacgc aatgcgcact aaaagggcat catttgatgc
      121 cctttttgca cgctttcgta ccagaacctg gctcatcagt gattttcttt gtcataatca
      181 ttgctgagac aggctctgtt gagggcgtat aatccgaaaa gctaatacgc gtttcgattt
      241 ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa acttctgaca ggttggttta
      301 tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct ggaagcgatg aagtgggtcg
      361 ttgtggtggc attgctcctg gcggcgattg tcggcaacta cctttatcgc gacattatgc
      421 tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc agcgggtggt gtcgcgctgt
      481 taacgacaaa aggtaaagct accattgctt ttgcccgtga agcgcgtacc gaagtccgta
      541 aggtcatttg gccgactcgc caggaaacat tgcacaccac gctgattgtg gctgcggtta
      601 ccgcagtaat gtcactgatc ctgtggggac tggatggtat tctggttcgc ctggtatcct
      661 ttatcactgg cctgaggttc tgagatgtct gaagctccta aaaagcgctg gtacgtcgtt
      721 caggcgtttt ccggttttga aggccgcgta gcaacgtcgc tgcgtgagca tatcaaatta
      781 cacaacatgg aagatttgtt tggtgaagtc atggtaccaa ccgaagaagt ggttgaaatc
      841 cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc ctggctacgt cctcgttcag
      901 atggtgatga acgacgcgag ctggcacttg gtgcgcagcg taccgcgtgt gatgggcttc
      961 atcggcggta cttccgatcg tcctgcgcca atcagcgata aagaagtcga tgcgattatg
     1021 aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa cgctgtttga accgggtgaa
     1081 atggtccgtg ttaatgatgg tccgttcgct gacttcaacg gtgttgttga agaagtggat
     1141 tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg gtcgtgcgac cccggtagag
     1201 ctggacttca gccaggttga aaaagcctaa cccagcgatc aaaaaagcgg cgatttaatc
     1261 gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt ttgtttttat gggccttgcc
     1321 cgtaaaacga ttttttatat cacggggagc ctctcagagg cgttattacc caacttgagg
     1381 aatttataat ggctaagaaa gtacaagcct atgtcaagct gcaggttgca gctggtatgg
     1441 ctaacccgag tccgccagta ggtccggctc tgggtcagca gggcgtaaac atcatggaat
     1501 tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa aggtctgccg attccggtag
     1561 taatcaccgt ttacgctgac cgttctttca ctttcgttac caagaccccg ccggcagcag
     1621 ttctgctgaa aaaagcggca ggtatcaagt ctggttccgg taagccgaac aaagacaaag
     1681 tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca gaccaaagct gccgacatga
     1741 ctggtgccga cattgaagcg atgactcgct ccatcgaagg tactgcacgt tccatgggcc
     1801 tggtagtgga ggactaagaa atggctaaac tgaccaagcg catgcgtgtt atccgtgaga
     1861 aagttgatgc aaccaaacag tacgacatca acgaagctat cgcactgctg aaagagctgg
     1921 cgactgctaa attcgtagaa agcgtggacg tagctgttaa cctcggcatc gacgctcgta
     1981 aatctgacca gaacgtacgt ggtgcaactg tactgccgca cggtactggc cgttccgttc
     2041 gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc taaagctgca ggcgcagaac
     2101 tggtaggtat ggaagatctg gctgaccaga tcaagaaagg cgaaatgaac tttgacgttg
     2161 ttattgcttc tccggatgca atgcgcgttg ttggccagct gggccaggtt ctgggtccgc
     2221 gtggcctgat gccaaacccg aaagtgggta ctgtaacacc gaacgttgct gaagcggtta
     2281 aaaacgctaa agctggccag gttcgttacc gtaacgacaa aaacggcatc atccacacca
     2341 ccatcggtaa agtggacttt gacgctgaca aactgaaaga aaacctggaa gctctgctgg
     2401 ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt gtacatcaag aaagttagca
     2461 tctccaccac catgggtgca ggtgttgcag ttgaccaggc tggcctgagc gcttctgtaa
     2521 actaatgcct ttacgtgggc ggtgattttg tctacaatct tacccccacg tataatgctt
     2581 aatgcagacg tatatccgag atattcgggt tgtggcaagg cggcaactga gtgagtcgcc
     2641 aagagcatag ctaactatgt gactggtgcg aatgaaggaa gccaacgccg ccacaagttg
     2701 aatagcgacg gatagaaaag atttgttcgt tggagcctgg cctatccagg cctccgtcga
     2761 agaccgcagg agtttcgcaa gaaacttaat cccctgcgta gacggtgaca gaacgctaag
     2821 attattcttt tatattctgg cttgtttctg ctcaccgtaa ttaagacgct ctctccgttt
     2881 ggaggagtga agtgagttcc agagattttc tctggcaaac atccaggagc aaagctaatg
     2941 gctttaaatc ttcaagacaa acaagcgatt gttgctgaag tcagcgaagt agccaaaggc
     3001 gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg tagataaaat gactgaactg
     3061 cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg ttcgtaacac cctgctgcgc
     3121 cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg cgtttgttgg tccgaccctg
     3181 attgcatact ctatggaaca cccgggcgct gctgctcgtc tgttcaaaga gttcgcgaaa
     3241 gcgaatgcaa aatttgaggt caaagccgct gcctttgaag gtgagctgat cccggcgtct
     3301 cagatcgacc gcctggcaac tctgccgacc tacgaagaag caattgcacg cctgatggca
     3361 accatgaaag aagcttcggc tggcaaactg gttcgtactc tggctgctgt acgcgatgcg
     3421 aaagaagctg cttaatcgca gttatctttt taacgcattc gcttacgtat aaacttattc
     3481 tgatattcag gaacaattta aatgtctatc actaaagatc aaatcattga agcagttgca
     3541 gctatgtctg taatggacgt tgtagaactg atctctgcaa tggaagaaaa attcggtgtt
     3601 tccgctgctg ctgctgtagc tgtagctgct ggcccggttg aagctgctga agaaaaaact
     3661 gaattcgacg taattctgaa agctgctggc gctaacaaag ttgctgttat caaagcagta
     3721 cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc tggtagaatc tgcaccggct
     3781 gctctgaaag aaggcgtgag caaagacgac gcagaagcac tgaaaaaagc tctggaagaa
     3841 gctggcgctg aagttgaagt taaataagcc aacccttccg gttgcagcct gagaaatcag
     3901 gctgatggct ggtgactttt tagtcaccag cctttttgcg ctgtaaggcg ccagtagcgt
     3961 ttcacactgt ttgactactg ctgtgccttt caatgcttgt ttctatcgac gacttaatat
     4021 actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa tggtttaagc gtgatagcaa
     4081 caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa tagtgttgca caaactgtcc
     4141 gctcaatgga cagatgggtc gacttgtcag cgagctgagg aaccctatgg tttactccta
     4201 taccgagaaa aaacgtattc gtaaggattt tggtaaacgt ccacaagttc tggatgtacc
     4261 ttatctcctt tctatccagc ttgactcgtt tcagaaattt atcgagcaag atcctgaagg
     4321 gcagtatggt ctggaagctg ctttccgttc cgtattcccg attcagagct acagcggtaa
     4381 ttccgagctg caatacgtca gctaccgcct tggcgaaccg gtgtttgacg tccaggaatg
     4441 tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt aaactgcgtc tggtgatcta
     4501 tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa gaacaagaag tctacatggg
     4561 cgaaattccg ctcatgacag acaacggtac ctttgttatc aacggtactg agcgtgttat
     4621 cgtttcccag ctgcaccgta gtccgggcgt cttctttgac tccgacaaag gtaaaaccca
     4681 ctcttcgggt aaagtgctgt ataacgcgcg catcatccct taccgtggtt cctggctgga
     4741 cttcgaattc gatccgaagg acaacctgtt cgtacgtatc gaccgtcgcc gtaaactgcc
     4801 tgcgaccatc attctgcgcg ccctgaacta caccacagag cagatcctcg acctgttctt
     4861 tgaaaaagtt atctttgaaa tccgtgataa caagctgcag atggaactgg tgccggaacg
     4921 cctgcgtggt gaaaccgcat cttttgacat cgaagctaac ggtaaagtgt acgtagaaaa
     4981 aggccgccgt atcactgcgc gccacattcg ccagctggaa aaagacgacg tcaaactgat
     5041 cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct aaagactata ttgatgagtc
     5101 taccggcgag ctgatctgcg cagcgaacat ggagctgagc ctggatctgc tggctaagct
     5161 gagccagtct ggtcacaagc gtatcgaaac gctgttcacc aacgatctgg atcacggccc
     5221 atatatctct gaaaccttac gtgtcgaccc aactaacgac cgtctgagcg cactggtaga
     5281 aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt gaagcagctg aaagcctgtt
     5341 cgagaacctg ttcttctccg aagaccgtta tgacttgtct gcggttggtc gtatgaagtt
     5401 caaccgttct ctgctgcgcg aagaaatcga aggttccggt attctgagca aagacgacat
     5461 cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt aaaggcgaag tcgatgatat
     5521 cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa atggcggaaa accagttccg
     5581 cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt ctgtctctgg gcgatctgga
     5641 taccctgatg cctcaggata tgatcaacgc caagccgatt tccgcagcag tgaaagagtt
     5701 cttcggttcc agccagctgt ctcagtttat ggaccagaac aacccgctgt ctgagattac
     5761 gcacaaacgt cgtatctccg cactcggccc aggcggtctg acccgtgaac gtgcaggctt
     5821 cgaagttcga gacgtacacc cgactcacta cggtcgcgta tgtccaatcg aaacccctga
     5881 aggtccgaac atcggtctga tcaactctct gtccgtgtac gcacagacta acgaatacgg
     5941 cttccttgag actccgtatc gtaaagtgac cgacggtgtt gtaactgacg aaattcacta
     6001 cctgtctgct atcgaagaag gcaactacgt tatcgcccag gcgaactcca acctggatga
     6061 agaaggccac ttcgtagaag acctggtaac ttgccgtagc aaaggcgaat ccagcttgtt
     6121 cagccgcgac caggttgact acatggacgt atccacccag caggtggtat ccgtcggtgc
     6181 gtccctgatc ccgttcctgg aacacgatga cgccaaccgt gcattgatgg gtgcgaatat
     6241 gcaacgtcag gccgttccga ctctgcgcgc tgataagccg ctggttggta ctggtatgga
     6301 acgtgctgtt gccgttgact ccggtgtaac tgcggtagct aaacgtggtg gtgtcgttca
     6361 gtacgtggat gcttcccgta tcgttatcaa agttaacgaa gacgagatgt atccgggtga
     6421 agcaggtatc gacatctaca acctgaccaa atacacccgt tctaaccaga acacctgtat
     6481 caaccagatg ccgtgtgtgt ctctgggtga accggttgaa cgtggcgacg tgctggcaga
     6541 cggtccgtcc accgacctcg gtgaactggc gcttggtcag aacatgcgcg tagcgttcat
     6601 gccgtggaat ggttacaact tcgaagactc catcctcgta tccgagcgtg ttgttcagga
     6661 agaccgtttc accaccatcc acattcagga actggcgtgt gtgtcccgtg acaccaagct
     6721 gggtccggaa gagatcaccg ctgacatccc gaacgtgggt gaagctgcgc tctccaaact
     6781 ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc ggtggcgaca ttctggttgg
     6841 taaggtaacg ccgaaaggtg aaactcagct gaccccagaa gaaaaactgc tgcgtgcgat
     6901 cttcggtgag aaagcctctg acgttaaaga ctcttctctg cgcgtaccaa acggtgtatc
     6961 cggtacggtt atcgacgttc aggtctttac tcgcgatggc gtagaaaaag acaaacgtgc
     7021 gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa gacctgtctg aagaactgca
     7081 gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg ctggtagccg gtggcgttga
     7141 agctgagaag ctcgacaaac tgccgcgcga tcgctggctg gagctgggcc tgacagacga
     7201 agagaaacaa aatcagctgg aacagctggc tgagcagtat gacgaactga aacacgagtt
     7261 cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag ggcgacgatc tggcaccggg
     7321 cgtgctgaag attgttaagg tatatctggc ggttaaacgc cgtatccagc ctggtgacaa
     7381 gatggcaggt cgtcacggta acaagggtgt aatttctaag atcaacccga tcgaagatat
     7441 gccttacgat gaaaacggta cgccggtaga catcgtactg aacccgctgg gcgtaccgtc
     7501 tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt atggctgcga aaggtatcgg
     7561 cgacaagatc aacgccatgc tgaaacagca gcaagaagtc gcgaaactgc gcgaattcat
     7621 ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa gttgacctga gtaccttcag
     7681 cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa ggtatgccaa tcgcaacgcc
     7741 ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg ctgaaacttg gcgacctgcc
     7801 gacttccggt cagatccgcc tgtacgatgg tcgcactggt gaacagttcg agcgtccggt
     7861 aaccgttggt tacatgtaca tgctgaaact gaaccacctg gtcgacgaca agatgcacgc
     7921 gcgttccacc ggttcttaca gcctggttac tcagcagccg ctgggtggta aggcacagtt
     7981 cggtggtcag cgtttcgggg agatggaagt gtgggcgctg gaagcatacg gcgcagcata
     8041 caccctgcag gaaatgctca ctgttaagtc tgatgacgtg aacggtcgta ccaagatgta
     8101 taaaaacatt gtggacggca accatcagat ggagccgggc atgccagaat ccttcaacgt
     8161 attgttgaaa gagattcgtt cgctgggtat caacatcgaa ctggaagacg agtaattctc
     8221 gctcaaacag gtcactgctg tcggggtaaa acccggcagc ggattgtgct aactccgacg
     8281 ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc gcagactaaa accgaagagt
     8341 ttgatgcgat caaaattgct ctggcttcgc cagacatgat ccgttcatgg tctttcggtg
     8401 aagttaaaaa gccggaaacc atcaactacc gtacgttcaa accagaacgt gacggccttt
     8461 tctgcgcccg tatctttggg ccggtaaaag attacgagtg cctgtgcggt aagtacaagc
     8521 gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt tgaagtgacc cagactaaag
     8581 tacgccgtga gcgtatgggc cacatcgaac tggcttcccc gactgcgcac atctggttcc
     8641 tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat gccgctgcgc gatatcgaac
     8701 gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg tatgaccaac ctggaacgtc
     8761 agcagatcct gactgaagag cagtatctgg acgcgctgga agagttcggt gacgaattcg
     8821 acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa gagcatggat ctggagcaag
     8881 agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc cgaaaccaag cgtaaaaagc
     8941 tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc tggtaacaaa ccagagtgga
     9001 tgatcctgac cgttctgccg gtactgccgc cagatctgcg tccgctggtt ccgctggatg
     9061 gtggtcgttt cgcgacttct gacctgaacg atctgtatcg tcgcgtcatt aaccgtaaca
     9121 accgtctgaa acgtctgctg gatctggctg cgccggacat catcgtacgt aacgaaaaac
     9181 gtatgctgca ggaagcggta gacgccctgc tggataacgg tcgtcgcggt cgtgcgatca
     9241 ccggttctaa caagcgtcct ctgaaatctt tggccgacat gatcaaaggt aaacagggtc
     9301 gtttccgtca gaacctgctc ggtaagcgtg ttgactactc cggtcgttct gtaatcaccg
     9361 taggtccata cctgcgtctg catcagtgcg gtctgccgaa gaaaatggcg ctggagctgt
     9421 tcaaaccgtt catctacggc aagctggaac tgcgtggtct tgctaccacc attaaagctg
     9481 cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga tatcctggac gaagttatcc
     9541 gcgaacaccc ggtactgctg aaccgtgcac cgactctgca ccgtctgggt atccaggcat
     9601 ttgaaccggt actgatcgaa ggtaaagcta tccagctgca cccgctggtt tgtgcggcat
     9661 ataacgccga cttcgatggt gaccagatgg ctgttcacgt accgctgacg ctggaagccc
     9721 agctggaagc gcgtgcgctg atgatgtcta ccaacaacat cctgtccccg gcgaacggcg
     9781 aaccaatcat cgttccgtct caggacgttg tactgggtct gtactacatg acccgtgact
     9841 gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc gaaagaagca gaacgtctgt
     9901 atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt gcgtatcacc gagtatgaaa
     9961 aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa agacacgact gttggccgtg
    10021 ccattctgtg gatgattgta ccgaaaggtc tgccttactc catcgtcaac caggcgctgg
    10081 gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg cattctcggt ctgaaaccga
    10141 ccgttatttt tgcggaccag atcatgtaca ccggcttcgc ctatgcagcg cgttctggtg
    10201 catctgttgg catcgatgac atggtcatcc cggagaagaa acacgaaatc atctccgagg
    10261 cagaagcaga agttgctgaa attcaggagc agttccagtc tggtctggta actgcgggcg
    10321 aacgctacaa caaagttatc gatatctggg ctgcggcgaa cgatcgtgta tccaaagcga
    10381 tgatggataa cctgcaaact gaaaccgtta ttaaccgtga cggtcaggaa gagaagcagg
    10441 tttccttcaa cagcatctac atgatggccg actccggtgc gcgtggttct gcggcacaga
    10501 ttcgtcagct tgctggtatg cgtggtctga tggcaaagcc ggatggctcc atcatcgaaa
    10561 cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact ccagtacttc atctccaccc
    10621 acggtgctcg taaaggtctg gcggataccg cactgaaaac tgcgaactcc ggttacctga
    10681 ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac cgaagacgat tgtggtaccc
    10741 atgaaggtat catgatgact ccggttatcg aggggggtga cgttaaagag ccgctgcgcg
    10801 atcgcgtact gggtcgtgta actgctgaag acgttctgaa gccgggtact gctgatattc
    10861 tcgttccgcg caacacgctg ctgcacgaac agtggtgtga cctgctggaa gagaactctg
    10921 tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac cgactttggt gtatgtgcgc
    10981 actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa caaaggtgaa gcaatcggtg
    11041 ttatcgcggc acagtccatc ggtgaaccgg gtacacagct gaccatgcgt acgttccaca
    11101 tcggtggtgc ggcatctcgt gcggctgctg aatccagcat ccaggtgaaa aacaaaggta
    11161 gcatcaagct cagcaacgtg aagtcggttg tgaactccag cggtaaactg gttatcactt
    11221 cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg taccaaagaa agctacaaag
    11281 tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca ggttgctggc ggcgaaaccg
    11341 ttgcaaactg ggacccgcac accatgccgg ttatcaccga agtaagcggt tttgtacgct
    11401 ttactgacat gatcgacggc cagaccatta ctcgtcagac cgacgaactg accggtctgt
    11461 cttcgctggt ggttctggat tccgcagaac gtaccgcagg tggtaaagat ctgcgtccgg
    11521 cactgaaaat cgttgatgct cagggtaacg acgttctgat cccaggtacc gatatgcctg
    11581 cgcagtactt cctgccgggt aaagcgattg ttcagctgga agatggcgta cagatcagct
    11641 ctggtgacac cttggcgcgt attccgcagg aatccggcgg taccaaggac atcaccggtg
    11701 gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc gaaagagccg gcaatcctgg
    11761 ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa aggtaaacgt cgtctggtta
    11821 tcaccccggt agacggtagc gatccgtacg aagagatgat tccgaaatgg cgtcagctca
    11881 acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat ttccgacggt ccggaagcgc
    11941 cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac tcgttacatc gttaacgaag
    12001 tacaggacgt ataccgtctg cagggcgtaa agattaacga taaacacatc gaagttatcg
    12061 ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg cagctccgac ttcctggaag
    12121 gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg cgaactggaa gcgaacggca
    12181 aagtgggtgc aacttactcc cgcgatctgc tgggtatcac caaagcgtct ctggcaaccg
    12241 agtccttcat ctccgcggca tcgttccagg agaccactcg cgtgctgacc gaagcagccg
    12301 ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa cgttatcgtg ggtcgtctga
    12361 tcccggcagg taccggttac gcgtaccacc aggatcgtat gcgtcgccgt gctgcgggtg
    12421 aagctccggc tgcaccgcag gtgactgcag aagacgcatc tgccagcctg gcagaactgc
    12481 tgaacgcagg tctgggcggt tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa
    12541 acccgcttcg gcgggttttt ttatgggtgt tattcagagg gcaaattctt cacggaaaat
    12601 attaagaaag gcagggcgaa acgaggaaga agcttttcgc cctggacaac attcctgctg
    12661 acggcactac cataaaaaaa cacaacgctt tcaacaacct tgcgtttttc atcttccgta
    12721 gcggctcgga ataaatttac gttcttacat tccgtctcat agtctttgcg aggcgcgtcc
    12781 cagatagctg tcccagtctt tccataccgg ctgcaaaccc tgagcggtta acacggcagc
    12841 aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt tccaactcgg ggtgattatc
    12901 ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg ttattgatcg ccagcggaat
    12961 aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt gacagttcaa tctccggtgc
    13021 aagcagtcgg aaggcgcaga tggtttgcac taactggcgt tcatccataa tcgacgcagg
    13081 ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag acagagtaac ggctttgcca
    13141 gtaatgctgt tgcagccata gcaaatgttc ggcaaccata tagcagtcaa cgcgccagtt
    13201 gtcggaaagg ccaattagcg cgccgaggcc tatcttatca atccccgcac gccccagccg
    13261 atccggcgtt tccagccgcc agaagaagtc ctgttttttg cctttcaaat gatggcgggt
    13321 ataagtcgcc tcgtgatatg tctcctgata aaccatcacc ccatccagac caagttgctt
    13381 taactcggcg tattccgtct ccgccagcgg ttgcacttcc atttgtagtg aagagaactg
    13441 ttcacgcagg gcggggagat gacgacgaaa gtaatccatc cccactttcg cctgatgttc
    13501 accagtgact aatagcagat gttcaaagcc catctcccgt atagcggcgc tttccctggc
    13561 aatatccgct tcatccagcg ttttgcgctt gatgcgatta ctcatggaaa atccgcagta
    13621 cgtgcagtcg ttagcgcaaa gattggaaag ataaagcggg acgtagaaac taactgtgtt
    13681 gccaaatcgc tgacgggtca gacgctgcgc ctgttgggcc agttgttcca gatagccact
    13741 ggcggcaggc gataacagcg ccatcatgtc gtcgcgggtg agttgcgagg catttagcgc
    13801 ccgctctacg tcagcagccg ttttgccgtt gatacgcagg cggatgtcgt cccagtccag
    13861 ttgtcgccag cgattgctga aggttttcat gccgatgcct ccagaaatcc ggtcagcggg
    13921 ctggtggcat gagcaaaata actgcggctg cccggtccgg actgacgtgc cagcaggcct
    13981 acttctaccg ccagacgaaa tgccttcgcc atgttgacgg gatcgtccgc gacggcaatc
    14041 gccgtattca ctaacaccgc gtcggccccc atttccagcg cctgcgtggc atggctggga
    14101 acgccgatgc cagcatcgac aaccaccgga actgtggcct gctggataat aatctccagc
    14161 atggcacggg tttccagtcc ctgattcgag ccaatcggcg cgccgagcgg catcaccgct
    14221 gcacagccga cttcttccag acgtttacac aataccggat cggccccgca gtaaggcagc
    14281 acgacaaatc cctgttgtac cagcgtttcg gcggctttca gggtttcgat gggatcgggc
    14341 aacagccagc gggcgtcagg gtgaatctcc aattttagcc agtttgtgcc taacgcttca
    14401 cgtgccagat gggcggcgaa aatggcttct tccgctgttt tcgccccgga agtattcggc
    14461 agcagggcca cacccgccgc gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag
    14521 tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg aagcgcggat cgcctccacc
    14581 atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg
    14641 tccgcaatac gtaacatctc aaccccctgc aataacctga aaaagcagga tctggtcgcc
    14701 atcctgcacg atatgttgcg tccactgctc acgcgggacg atttgctgat taatcgccag
    14761 agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc
    14821 ggcgcactgc atcggttgat cgttaaacag gatctgcatt gcttcctccg cataccgggc
    14881 aaccactggc gcggcgcaac gccaggctgc gccactggct cgacttaccg tcgaacagtc
    14941 ggagttctcc cgcaggcgtc tctataccgc ttaataactt aatggcttcc agtgcctgca
    15001 aagtgcccat caccccgacc accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg
    15061 gctgctggtt atctggccac aggcagcggt aacacccctg ctcccagggc ggtgtcagta
    15121 ccatcaactg accgccaaat ccgaccgcgc tggcggtgat aagcggcgtg ttgagtgcca
    15181 cgcaggcggc attaatctcc tggcgagtcg ccatattgtc ggtacagtcg agcaccacat
    15241 cggcttgtgc aaccgcatct ttcaacgcct cacccgttaa ccgttgttgt aatgctgtca
    15301 gttgaatgtc gggattcaac tgtgtcagtc gctgttggct gacctgcgat ttcgggctgt
    15361 tgatatcttc agtggtaaag aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt
    15421 catctgccag taccagcgtc ccgacgccag cccctgccag gtatagcgca gcaggtgtac
    15481 ccagcccgcc aagaccaata atcagcacct ggctgtcgag cagtttttgc tgcccgtcaa
    15541 gagcgatatc gtcgagcaga atttggcggc tataacgcat aaagtcacgg tcattcatcg
    15601 ccaactcctg caatttccag caactgtgcc gttgccaaac gccagtctgc ggcttgagta
    15661 atggcgctga cgacggcgat actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc
    15721 agactgatac cgccaatcgc cacggtggga taatccgcca ggcgctcaac atgccgtgcc
    15781 aactgttcca gcccctgcgg tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt
    15841 cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt
    15901 gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa
    15961 tcttcctgcc ccaaatggac gccatacgcc tgatgcttga tcgcgagccg ccagtaatcg
    16021 ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa ttgccgccac gacatcggct
    16081 tccacctctt catcgcgccg atctttgatg cgtagctgga gagtacgtac gcctgcatcc
    16141 aacagacgtt cgatccactg tacgctgtcc accaccgggt acagtcctaa acgaaaaggt
    16201 acaggaggaa aatcaggctg atacatcacg cttcctcctt atgcaggtag atttctccgc
    16261 ctctggcacg gaagttctcc gacatatccg ccattcccac ttcaatggtt tgcgcggcgg
    16321 cgtaatcgcg tacttcctgg ctgattttca tcgagcagaa ttttggccca cacatggagc
    16381 aaaaatgggc gactttgccg gactcttggg gcagggtttc atcgtgataa gcgcgggcgg
    16441 taaacgggtc gagggccaga ttaaactggt cttcccagcg aaattcgaag cgggctttcg
    16501 acatggcgtt atcgcgaatt tgcgcgcccg gatgcccttt tgccaggtca gcagcgtggg
    16561 cggcaatctt ataagtgata agcccctgct taacatcttc tttattgggc agacccagat
    16621 gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa ccagccaatc atcgccgcac
    16681 caatccccga cgtgaagtgg tcatagcccg gtgcaatatc ggtagttagc ggccccagag
    16741 tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt catattgcgg cggatcatct
    16801 gcatcggcac atggcctggg ccttcaatca tcacctgtac gtcatattcc caggcgattt
    16861 tggtcagttc gcccagcgta tgcaattcgg caaactgcgc ttcatcgttg gcgtcctgaa
    16921 tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac gtcataagcg gcacagattt
    16981 cacaaatttc gcggaagtgt tgatagagga aattttcctg atgatgggag aggcaccatt
    17041 tcgccataat cgaaccgccg cgagagacga taccggtcag gcgtttcgcg gtcatcggca
    17101 catagcgcag cagtacgccc gcatggatag tgaagtaatc cacaccttgc tcggcctgtt
    17161 ccagcagcgt gtcgcggaac gcttcccagg taagatcttc ggcgatcccg ttaaccttct
    17221 ccagcgcctg gtagatcggc actgtaccga tcggcaccgg actgttacgc aaaatccact
    17281 cgcgggtttc gtgaatatag cgaccggtgg agagatccat caccgtatcc gctccccagc
    17341 gcgtggacca taccagcttt tccacttctt cttcgatgga agaggtgacc gccgagttgc
    17401 cgatattggc gttaactttt accaggaagt tacggccaat aatcatcggc tccgattccg
    17461 gatgattaat gttggccggg ataatcgcac gtccggcagc aacttcatca cggacaaatt
    17521 ccgcagtgat attttccggc agatgtgcgc caaagctcat tcccggatgc tggtggcgta
    17581 aaacctcgct acggatgcgc tcgcggccca tattctcgcg gatggcgatg aattccattt
    17641 ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt gacacggcgt cctgttttgg
    17701 cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc gagaccatca tctgccagcc
    17761 gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc ggtatcggcg cgcgcatcga
    17821 tccacggctg gcgtagtttt gccagccctt gctgcacgtt aatggcgatc tgcggatcac
    17881 catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt
    17941 ctttgctacc gccaattagc gtcgggctaa gctggatctc acgcatcggc acgcgcacgc
    18001 cggggtgtgt gccagtgata taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg
    18061 tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca
    18121 tagctcattc caaaaagtta aggacgtggc ttgtcagacg acggatgaag caagagacga
    18181 tcgccgcaaa ggcgatgcga tagcagatta actcttgttc ccttcgcagg tattagcctg
    18241 atcaggttcc gcggatcccg aataaacggt ctcagccagt taaggcactc cgacaagaaa
    18301 ttagccccca caaacggggc gttgaatgta aattacgcgt taacagcgca gaactcaagc
    18361 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    18421 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    18481 atgatcgatg gcggtttcca ggctggaatc gtagtaatcc ataatctgtt gggtattggc
    18541 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    18601 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    18661 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    18721 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    18781 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    18841 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    18901 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    18961 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    19021 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    19081 acctgtttgg ttaatacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    19141 tgatctcgat gttgggctgt ttcaactggc cggacgtggc gtgcaactgg cggagtttta
    19201 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    19261 ggcgatgctg tgtagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    19321 tgttgccatc cgtcgcgatg attcgctcct cctcgcccag catacccgcc atcgtaacgg
    19381 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    19441 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    19501 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    19561 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    19621 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatactg tggcgatgtg
    19681 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    19741 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    19801 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    19861 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    19921 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    19981 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    20041 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgagct
    20101 gggttacgtg atgaacgcgg tgcgtaccat tcgtcacgaa ctgaaaggcg aagtgccgct
    20161 gattggtttt tccggcagcc cgtggacgct ggcaacctac atggtggaag gcggcagcag
    20221 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    20281 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    20341 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    20401 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    20461 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggctga
    20521 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    20581 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    20641 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    20701 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    20761 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taagagcctg atccatcagg
    20821 ctattttatt tgccattttg aggccgggca gtgctcaaaa tcctcacgta ctacgtgtac
    20881 gctccggttt ttccgcgctg tccggcctca aactgccttc aacaattacg cctgttgaac
    20941 caggctctaa ggagcgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21001 ttcttctgtg atctgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21061 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21121 tccctcgctt gagctggtgg agtataaagt tgcccgcatc gccaccacca tgccttacat
    21181 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21241 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg
    21301 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    21361 gctctgcggt aaattcgaac cgctctccag caaaccgggc gcgctggccc cactgatgga
    21421 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    21481 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    21541 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    21601 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    21661 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    21721 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    21781 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgtattctcg atctcatctg
    21841 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    21901 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    21961 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22021 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22081 tcgcgaaatg ggcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22141 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22201 aggccttagg gcagacctgc gtgagtcggg tgagagcaat attggtataa tttttcagca
    22261 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    22321 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    22381 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    22441 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    22501 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    22561 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc atcgcgctga
    22621 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    22681 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    22741 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    22801 ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc cctttaccgc
    22861 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    22921 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    22981 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23041 gaacattcgc gcgcgttttg acgatcgcgg cgatctgagc tttatgcaac gtgaatccga
    23101 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca
    23161 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23221 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    23281 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    23341 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    23401 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    23461 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    23521 gccatcgcga tatctcgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    23581 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    23641 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    23701 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    23761 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    23821 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    23881 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    23941 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24001 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24061 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24121 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24181 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24241 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    24301 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    24361 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    24421 gcatggtggg aaaacagctt tattcccctc aggaaatgca gcagttacat ccgggagatg
    24481 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    24541 agtttcaacc gatgtttgct gctggaatgt accggatgcg ccatatgcag cagtatgccg
    24601 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    24661 gtgagcagag aaacaccctg attattctct tcgccctggc gacggtcttg ctggcaagcg
    24721 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    24781 tgaagcgcaa agagaagctg gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    24841 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    24901 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    24961 gcgtggtaag cgagttgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25021 atctcaacac gctgattaac cactcattac agctggtaag ccaggatgca aacagccggg
    25081 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25141 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25201 gcgtgattag cgtgacggcc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25261 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    25321 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    25381 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    25441 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    25501 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    25561 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggaac aggtttttga
    25621 tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc atcgccacgc tgaaagagat
    25681 caaagcgtta aacccggcaa ttccggtgct gattatgact gcgtactcca gcgtcgagac
    25741 ggcggtagag gcactgaaaa ctggggcgca ggattatctc atcaagccgc tggatttcga
    25801 taacctacag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    25861 acctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    25921 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    25981 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26041 accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat ctgaattgtt
    26101 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26161 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26221 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    26281 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    26341 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    26401 gctgcggcaa cggcgggaag atatccctct gctggctggc cattttcttc agcgctttgc
    26461 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    26521 ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg gtggaacggg cggtggtgct
    26581 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    26641 cccgctgggg caaagtcagg atattcagcc gctggtggaa gtggaaaaag aggtgattct
    26701 ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    26761 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tggcgcgcca
    26821 accgatatct ttccggcaga agcaatcatc ccagtggata tcggtcatta aggcgtaggc
    26881 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    26941 gctggttacc acctgctcgt catccgccag ttttgtgccc gcgtggaaca ctttgccgtc
    27001 agccacttct tccagcggca ggccgtggat cacatcgccg gtgcggtaat cgcccggata
    27061 tccacccgca gccatcacca cgctgagaga agcacgttca tcccactctg acgttttctc
    27121 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27181 cataatcggc tgggtttccg gatcgccaaa gcggcagtta aattcaataa ccttcggatt
    27241 gccctgtttg tcgatcatca ggcccgcata gagaaaaccg gtgtaggtgt tgccttccgc
    27301 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    27361 ggttactacc ggcgcggggg agtaagcgcc catcccgccg gtgtttggtc cgttatcttt
    27421 atcgcctacg cgtttgtgat cctggctggt agccatcggc agcacatgct cgccgtccac
    27481 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    27541 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    27601 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    27661 cgcgcctttc tcacgcagat acaccagcgc aggttctacc tcggtgaagt tctggtattc
    27721 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    27781 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    27841 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    27901 tgcgaaatcc aacagcgccg ggatatcggt cacgccgata gcaacgtttt gcagcgcggg
    27961 ttccagcgca gtgcctgcat tacccggagc agcaaaaaca gtctcaacca gcggcgactg
    28021 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28081 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28141 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28201 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28261 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    28321 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    28381 tagacgatag cattggattt cacgaatttc gccactttcc agcagaacag cgcatcacgc
    28441 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    28501 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    28561 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    28621 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    28681 atggcctgtg cggtttccgc atccagctcg cggttaaagg caatgatgcc gccgaatgcg
    28741 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    28801 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    28861 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    28921 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    28981 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29041 cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29101 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29161 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29221 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    29281 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcagccttca
    29341 cgagcaacag tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    29401 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    29461 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    29521 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    29581 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    29641 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctgggtttg
    29701 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    29761 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    29821 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    29881 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    29941 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30001 aatgcgcctt catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30061 aaga
//
LOCUS       DAJTUM010000043        29787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTUM010000043 DAJTUM010000000
VERSION     DAJTUM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            46..840
                     /locus_tag="OO471_002069"
     CDS             46..840
                     /locus_tag="OO471_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6243343.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            862..2271
                     /locus_tag="OO471_002070"
     CDS             862..2271
                     /locus_tag="OO471_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243344.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2249..3028
                     /locus_tag="OO471_002071"
     CDS             2249..3028
                     /locus_tag="OO471_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6243345.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3025..3885
                     /locus_tag="OO471_002072"
     CDS             3025..3885
                     /locus_tag="OO471_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6243346.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4033..4608)
                     /locus_tag="OO471_002073"
                     /pseudo
     CDS             complement(4033..4608)
                     /locus_tag="OO471_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4625..4885)
                     /locus_tag="OO471_002074"
     CDS             complement(4625..4885)
                     /locus_tag="OO471_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6243347.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4876..6147)
                     /locus_tag="OO471_002075"
     CDS             complement(4876..6147)
                     /locus_tag="OO471_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6243348.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6225..6587)
                     /gene="queD"
                     /locus_tag="OO471_002076"
     CDS             complement(6225..6587)
                     /gene="queD"
                     /locus_tag="OO471_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6243349.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6906..8705
                     /gene="cysJ"
                     /locus_tag="OO471_002077"
     CDS             6906..8705
                     /gene="cysJ"
                     /locus_tag="OO471_002077"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6243350.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPMIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8705..10417
                     /gene="cysI"
                     /locus_tag="OO471_002078"
     CDS             8705..10417
                     /gene="cysI"
                     /locus_tag="OO471_002078"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6243351.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10491..11225
                     /gene="cysH"
                     /locus_tag="OO471_002079"
     CDS             10491..11225
                     /gene="cysH"
                     /locus_tag="OO471_002079"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6243352.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11489..11641
                     /locus_tag="OO471_002080"
     CDS             11489..11641
                     /locus_tag="OO471_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6243353.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11835..12926
                     /locus_tag="OO471_002081"
                     /pseudo
     CDS             11835..12926
                     /locus_tag="OO471_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13072..14109)
                     /gene="iap"
                     /locus_tag="OO471_002082"
     CDS             complement(13072..14109)
                     /gene="iap"
                     /locus_tag="OO471_002082"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6243354.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14361..15269
                     /gene="cysD"
                     /locus_tag="OO471_002083"
     CDS             14361..15269
                     /gene="cysD"
                     /locus_tag="OO471_002083"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6243355.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15271..16698
                     /gene="cysN"
                     /locus_tag="OO471_002084"
     CDS             15271..16698
                     /gene="cysN"
                     /locus_tag="OO471_002084"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6243356.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16698..17303
                     /gene="cysC"
                     /locus_tag="OO471_002085"
     CDS             16698..17303
                     /gene="cysC"
                     /locus_tag="OO471_002085"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6243357.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17353..17676
                     /locus_tag="OO471_002086"
     CDS             17353..17676
                     /locus_tag="OO471_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6243358.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17870..18181
                     /gene="ftsB"
                     /locus_tag="OO471_002087"
     CDS             17870..18181
                     /gene="ftsB"
                     /locus_tag="OO471_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6243359.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18200..18910
                     /gene="ispD"
                     /locus_tag="OO471_002088"
     CDS             18200..18910
                     /gene="ispD"
                     /locus_tag="OO471_002088"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6243360.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18910..19389
                     /gene="ispF"
                     /locus_tag="OO471_002089"
     CDS             18910..19389
                     /gene="ispF"
                     /locus_tag="OO471_002089"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6243361.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19386..20435
                     /gene="truD"
                     /locus_tag="OO471_002090"
     CDS             19386..20435
                     /gene="truD"
                     /locus_tag="OO471_002090"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6243362.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20416..21177
                     /gene="surE"
                     /locus_tag="OO471_002091"
     CDS             20416..21177
                     /gene="surE"
                     /locus_tag="OO471_002091"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6243363.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21171..21797
                     /gene="pcm"
                     /locus_tag="OO471_002092"
     CDS             21171..21797
                     /gene="pcm"
                     /locus_tag="OO471_002092"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6243364.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21937..23076
                     /gene="nlpD"
                     /locus_tag="OO471_002093"
     CDS             21937..23076
                     /gene="nlpD"
                     /locus_tag="OO471_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6243365.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23139..24131
                     /gene="rpoS"
                     /locus_tag="OO471_002094"
     CDS             23139..24131
                     /gene="rpoS"
                     /locus_tag="OO471_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6243366.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24251..24658)
                     /locus_tag="OO471_002095"
     CDS             complement(24251..24658)
                     /locus_tag="OO471_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6243367.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24805..25398
                     /locus_tag="OO471_002096"
     CDS             24805..25398
                     /locus_tag="OO471_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6243368.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25398..26345
                     /locus_tag="OO471_002097"
                     /pseudo
     CDS             25398..26345
                     /locus_tag="OO471_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26618..27181)
                     /locus_tag="OO471_002098"
     CDS             complement(26618..27181)
                     /locus_tag="OO471_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243369.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27346..27597
                     /locus_tag="OO471_002099"
     CDS             27346..27597
                     /locus_tag="OO471_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6243370.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27599..29695
                     /locus_tag="OO471_002100"
     CDS             27599..29695
                     /locus_tag="OO471_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6243371.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7293 a         7381 c         8060 g         7053 t
ORIGIN      
        1 atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa aatctttaaa
       61 tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa aaccggcttc
      121 aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg tttgtatgac
      181 gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt gctgatcttt
      241 atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc ggaaatcgtt
      301 gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg gtttaacaac
      361 ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa aaccggcaac
      421 atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat ctacgaaagc
      481 gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat gctgggcggt
      541 cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga ctacaacgtt
      601 gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa caaaatcatc
      661 tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat tggtaaacat
      721 cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga agggctgaaa
      781 aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat tgaaaaataa
      841 tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc aggcgagaag
      901 ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt gcgaatggat
      961 gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg cgcacacctg
     1021 accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac gcccgccatg
     1081 caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg tttgtttctt
     1141 ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat cttcaccttc
     1201 agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac gccagagcat
     1261 cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc agtaggtcac
     1321 accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg cgcattcagc
     1381 gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt tatttccgaa
     1441 aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt gttgattacg
     1501 ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg gcgttttgca
     1561 gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg cgatgaagtg
     1621 gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg gcggcgcacg
     1681 gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat ttatacctgg
     1741 ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag cctgatgctt
     1801 aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct gctggcacat
     1861 cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt aatggcctgt
     1921 ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac caccatttcg
     1981 gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga cattcgctcg
     2041 ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag tactggcctg
     2101 ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct agcgacagtg
     2161 ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc cctcccgctg
     2221 gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta aagccgaacc
     2281 ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta atagcggacc
     2341 ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg ccgcgctgct
     2401 gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca tgggggatga
     2461 acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag ctgtattgct
     2521 ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata ttgccgaatg
     2581 gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg aagggcagaa
     2641 tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca cccaggtgga
     2701 gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac atgggttgcg
     2761 ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg aagtggcctt
     2821 gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta tccgtaaaac
     2881 tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag agcaacgacg
     2941 cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc tatggcagga
     3001 ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa tcaggagaat
     3061 gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct ggcgcactgg
     3121 caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt ggaacagtgg
     3181 cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga tgaactgtcg
     3241 acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc gttagatatt
     3301 ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac gctgcaaaca
     3361 gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa aaacgccacg
     3421 ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga ctggctggtt
     3481 agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag acgggtgctg
     3541 gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc agaaaagctg
     3601 ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga tgctgaaaaa
     3661 gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt tggcgcttcc
     3721 ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc gattaatcat
     3781 gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga ctggaaagtg
     3841 gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc tgccggatga
     3901 tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt aagcgtcgca
     3961 tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat tcaattggtt
     4021 acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat tcgtggtgga
     4081 aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc ataccagccc
     4141 gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt tgggcatgca
     4201 gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat caatgttgtg
     4261 aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt ccgctcttgc
     4321 cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca ccagtttcaa
     4381 aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat gaataaaagc
     4441 atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga tattgccgta
     4501 caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg cattatcttt
     4561 aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa attatcctta
     4621 ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc cactgttgta
     4681 gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg cgatagtcaa
     4741 tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg ttaatcaacc
     4801 gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca tcagcaacgc
     4861 gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg ccagatgcca
     4921 caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa caggtttatc
     4981 accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg cccgatacct
     5041 gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat cccatagcag
     5101 gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc ggtgctggca
     5161 ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat ccatgccgcg
     5221 cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca gccagccgtt
     5281 accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat gcgcaccata
     5341 ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt taaagcgagt
     5401 cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc aaacaatccc
     5461 taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt catcacagat
     5521 tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac gttcttcaat
     5581 agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt cgccagcagg
     5641 acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg ccagcaccac
     5701 ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc cgttttcttc
     5761 atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt cggcttcggc
     5821 aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc cgggctgtaa
     5881 gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt gagtgatgcg
     5941 tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg catgggtata
     6001 taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga gtaacaaaac
     6061 ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac cggcaccaat
     6121 aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac acaacaccct
     6181 aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc gccgcgatag
     6241 atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg cacaacgggt
     6301 ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc cagacctgga
     6361 atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc cgctttcagt
     6421 tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat ttccagtcgc
     6481 accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac atgtggtaag
     6541 cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat aatttctcta
     6601 caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt atctaaaacg
     6661 actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat ttgtttttca
     6721 ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt agtcgatttg
     6781 gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta accttacctt
     6841 ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact ggaacataac
     6901 gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg gagcaactgg
     6961 cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg gtttctggct
     7021 atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca gcgcccgccg
     7081 cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg cgccgggttg
     7141 ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg gtgaacgcgg
     7201 gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg acgtcaacgc
     7261 aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg ttctccaaaa
     7321 aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat agctcttatg
     7381 aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg gggggtgaac
     7441 gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc gagtggcgcg
     7501 cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg caatccgtcg
     7561 ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg ccgctggtgg
     7621 ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac gttcgccata
     7681 tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg ctgggcgtct
     7741 ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg ctgacaggcg
     7801 atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta cagtggcact
     7861 tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt acccgcagtg
     7921 aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc gcgacgacgc
     7981 tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg ctaattaatc
     8041 tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg gaagtcgaga
     8101 acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc gcccgtgccg
     8161 gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc cgcgtattta
     8221 tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccgatg attatgattg
     8281 gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc gctgacgaag
     8341 cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac ttcctgtatc
     8401 aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat ctcgcctggt
     8461 cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag ggcgcggagc
     8521 tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct aatcgcatgg
     8581 cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc atggacaccg
     8641 aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag cgagatgtct
     8701 actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga cagacgccga
     8761 gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt taaacgacgg
     8821 tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg gcatgtatca
     8881 gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc cgcgccacgc
     8941 gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt ggcaggcgat
     9001 cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca accgccagac
     9061 gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga tgctgcactc
     9121 ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac tctgcacctc
     9181 gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga agatttctga
     9241 acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg aaaaagtcgc
     9301 caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt tcaaaaccac
     9361 ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga acttcgtggc
     9421 gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg ggctttctat
     9481 cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct atctgccgct
     9541 agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact ggggtaaccg
     9601 aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg ttgagacgtt
     9661 taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc cgtatgaatt
     9721 tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact ggcacctgac
     9781 gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga aaactggcct
     9841 gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc agaatctgat
     9901 catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca aagagagcgg
     9961 gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt cattcccgac
    10021 ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg acaacatcga
    10081 taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg taacaggctg
    10141 cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta aagcaccggg
    10201 tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac ggatgtataa
    10261 agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag ggcgctgggc
    10321 gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg gcatcattcg
    10381 cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt cttgtaggcc
    10441 tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct atgtccaaac
    10501 tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg ctggcagaaa
    10561 ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg ctggataatc
    10621 tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg agcctgcatc
    10681 tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc tatctgttcc
    10741 cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac ctgaaagtgt
    10801 accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg tgggaacagg
    10861 gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg aaccgggcgc
    10921 tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa tccggcagcc
    10981 gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg ccgattatcg
    11041 actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa tatcacccat
    11101 tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa tgggaacccg
    11161 gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga ttacacgaag
    11221 ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc gtttttattt
    11281 tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac aggtatagaa
    11341 tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca gatggacaaa
    11401 agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca attcggatgt
    11461 tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg tggcaatcat
    11521 cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt tatgtgagtt
    11581 gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat cgaagaagta
    11641 gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc tcctgcaccc
    11701 tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat tatgaatttc
    11761 aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata tatatcatag
    11821 atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa aatattgtga
    11881 ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc gacgactatc
    11941 accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg gttaaaagaa
    12001 attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag gaacaggcag
    12061 ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc cgctcttttc
    12121 aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa aattacgaaa
    12181 aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt gaatgtcagg
    12241 agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata gaaatgtgga
    12301 tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa ttggataatt
    12361 ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag ccactgtttc
    12421 cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt ataaaacaac
    12481 tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg tcaacgcgat
    12541 tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag gctttagttc
    12601 aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct ttcaccggaa
    12661 ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag gtgctggatc
    12721 tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc ggcgcaggta
    12781 aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac gctcaggggc
    12841 tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga ctggtcaaaa
    12901 cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata aaccggcgca
    12961 atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat gggtatcact
    13021 tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc atcaagacgc
    13081 cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc agcgacgttc
    13141 tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta cgtcatgcca
    13201 gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat ccttatttcc
    13261 aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt cgaatatttc
    13321 tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg gattggtcgt
    13381 tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta atttccttac
    13441 tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa ttaagttatc
    13501 gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct tgagtaaatt
    13561 ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa taccatactc
    13621 ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac ctgcggcgtt
    13681 atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag catcgctcag
    13741 cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt tgccttcatg
    13801 agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat cgcgggcggt
    13861 ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt gctgaaactg
    13921 ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc gtcccggaaa
    13981 gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag gtgacgaggc
    14041 gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt ggcgcaatgc
    14101 ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata gtatgaaact
    14161 gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt gccttaagca
    14221 ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca ttctaattgg
    14281 taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc
    14341 ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg caactggagg
    14401 cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg gtgatgctct
    14461 actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg ttttatccag
    14521 gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc gagatgtatg
    14581 agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat aaaaacccgg
    14641 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta
    14701 tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg
    14761 gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct ttccgtgacc
    14821 gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac tacaacgggc
    14881 aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc gagcaggata
    14941 tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc gctgcggaac
    15001 gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc
    15061 aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc
    15121 tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg
    15181 tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg ggatccatgg
    15241 agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca acaaatcgcc
    15301 aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct gctgcgtttt
    15361 ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct gctgcacgat
    15421 acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa gcgtcacggc
    15481 acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc tgagcgcgaa
    15541 cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg taaatttatt
    15601 atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg cgcatcgaca
    15661 tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca aacccgtcgt
    15721 cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat caacaaaatg
    15781 gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta tctgaccttt
    15841 gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc actggaaggt
    15901 gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac actgctcgaa
    15961 gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg cttcccggtg
    16021 cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct ggcatccggt
    16081 cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc aaacgtcgcg
    16141 cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc gatcaccctg
    16201 gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc agacgaagcg
    16261 ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca gccgctttcc
    16321 ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg tgttgatggc
    16381 attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa cctgccgctg
    16441 aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga tcgctatcag
    16501 caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt gaccgtgggt
    16561 gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga attcagtgca
    16621 ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg cgcgcgcgat
    16681 ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat agtcatccgg
    16741 tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg tggtttaccg
    16801 gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg ttacataaac
    16861 tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta tgcagcgatc
    16921 tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa gtggcgaatt
    16981 tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac cgcgccgaac
    17041 gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt gtcgatacgc
    17101 cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg cgtgccggtg
    17161 aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg gcagaaattc
    17221 atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat ctgttgagac
    17281 agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt cgtcaaagta
    17341 acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga cagccttact
    17401 gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat ctcctggtta
    17461 tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt ctttctctac
    17521 acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat tgcgctgcat
    17581 tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac agtaggcatt
    17641 atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg tctcctctgt
    17701 tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg gtatacgtaa
    17761 caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca gaggatgtag
    17821 agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga tgggtaaact
    17881 aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt tcggtaagaa
    17941 cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag ctacaaacgc
    18001 gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca atggcggcca
    18061 ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg gcgaaacttt
    18121 ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa acaatcgata
    18181 aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg tggttccggc
    18241 ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct caatcggtaa
    18301 tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg tgacacgtgt
    18361 cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg cgaatcatcc
    18421 gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg caggtctgaa
    18481 agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt gtttgcatca
    18541 ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag ggatcctcgc
    18601 cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca ttgctcatac
    18661 cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc gtgagctgtt
    18721 acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg aagcctcggc
    18781 gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata acattaaagt
    18841 cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca tccatcagga
    18901 gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg tgaaggccca
    18961 attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca ttctgatggc
    19021 gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct gggggatatc
    19081 ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg cgagctgcta
    19141 cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt cgatgtcact
    19201 atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt gtttattgcc
    19261 gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac ggaaaaactt
    19321 ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact cattaaggca
    19381 acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa ggcaccgggc
    19441 tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt gagcctgatg
    19501 gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc cgttttgtgg
    19561 cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc gctgggcaaa
    19621 aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc aaggaaatgc
    19681 ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg cggcacaagc
    19741 gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg cgcgaagtga
    19801 gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt gtaccgaact
    19861 acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg ctgcgctggg
    19921 cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg tcggcagccc
    19981 gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac gttaatcaag
    20041 ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta gcaaccaccg
    20101 aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata accgccgcat
    20161 tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa caagcagctg
    20221 tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa gccgcgcgca
    20281 gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac gtcaccgtag
    20341 agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg gaacttatca
    20401 acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca tgcacccggt
    20461 atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt cgcccccgat
    20521 cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg cacgtttacc
    20581 tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt ctatcttggc
    20641 gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa cgccgggccg
    20701 aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga aggccgtcat
    20761 ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga cactgccgcg
    20821 gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac cggacgtatt
    20881 cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg cgtgacgcgc
    20941 tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg cggcaatacg
    21001 ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac cgattttgct
    21061 gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac tgcgcatagc
    21121 gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca atggtaagca
    21181 gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat gagcaggtgc
    21241 tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt gaacaaaaag
    21301 cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag ccgtatatgg
    21361 tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg gaaattggca
    21421 ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt tgctcggttg
    21481 aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt gatttacata
    21541 atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg ccgtttgacg
    21601 ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg cagctggacg
    21661 aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa cgggtgcgtc
    21721 gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc cctttagtga
    21781 agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta ttttgccgta
    21841 ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca cggtaattat
    21901 gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag cccaaaattc
    21961 accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg ctgttctgac
    22021 acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc aaatactaat
    22081 tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc gcagcaaccg
    22141 caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca aatccagcca
    22201 gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt ctataaccgt
    22261 cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc
    22321 gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc
    22381 aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt gggtaatgct
    22441 tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga
    22501 gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg
    22561 tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccaact
    22621 gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccgactgtc
    22681 agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg
    22741 atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa
    22801 ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa cgcgctgcgc
    22861 ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat
    22921 aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc
    22981 atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa
    23041 tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt
    23101 gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag
    23161 ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa
    23221 aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc
    23281 agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag attggttatt
    23341 caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg
    23401 tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc
    23461 gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac ttggggctga
    23521 tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca tacgcaacct
    23581 ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact attcgtttgc
    23641 cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag ttgtcccata
    23701 agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag ccagttgatg
    23761 acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc ccgctgggtg
    23821 gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac ggtccggaag
    23881 ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc gagctgaacg
    23941 ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa gcggcaacac
    24001 tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag attcaggttg
    24061 aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc gaagcgctgt
    24121 tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg gcctttttct
    24181 tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt cctacccggc
    24241 aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc tcctgctctt
    24301 ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt atcgccgcag
    24361 caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg tcagcgacgt
    24421 catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct gctagcgttg
    24481 ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt ttatcagcaa
    24541 tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc tgccagcgag
    24601 ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt aacgccatgt
    24661 aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga agaagatgtt
    24721 gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat aacgttcgca
    24781 tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg ggctaccggt
    24841 gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt cgagactcat
    24901 ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta cagcgctcgc
    24961 gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc gatcatctca
    25021 tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa aacgctcgcc
    25081 ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt cgtgctcaaa
    25141 gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat ccatctcgaa
    25201 aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc tgccttttat
    25261 aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt gctggatcaa
    25321 tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc ccggaatttt
    25381 tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag gcgcttgatg
    25441 accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat ctggcagcag
    25501 cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt gataatattc
    25561 gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag aaccacgcga
    25621 tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag tttatccgcc
    25681 gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg gcgcagaaca
    25741 ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc ctgaatgatg
    25801 gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg ctcgacccgg
    25861 ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc aagcgtaagc
    25921 tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa gcagaagagc
    25981 gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc acgctgatgg
    26041 gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg ctgcgtgaaa
    26101 gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg ggttcagaag
    26161 tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg ttcggtgagt
    26221 ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat aaagtctctt
    26281 atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc gaaatcgact
    26341 acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag acgggaatag
    26401 attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc aaaaatgtta
    26461 aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa tagcctttaa
    26521 agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa gcgccaaaac
    26581 caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct gctttctgag
    26641 gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta accttttcgt
    26701 ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga gcgtgcaact
    26761 cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc ccaagaacat
    26821 cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga atttgagcat
    26881 caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca acatcaccct
    26941 ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt tttaactgat
    27001 acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa gtgccttcat
    27061 tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact aacctaaaga
    27121 ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt gcttttctca
    27181 ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa taaaccataa
    27241 aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc catacggata
    27301 tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag acaaagagaa
    27361 gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga taacgggtgg
    27421 tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact gaaagcggta
    27481 gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg tgtaccgcca
    27541 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc gtcgtgatat
    27601 gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag ccaccacaaa
    27661 aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta gtcgccgtcg
    27721 ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa cacgaaaggc
    27781 actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt ctgaagtgaa
    27841 agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag catatcgcgg
    27901 ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc gctgggtatc
    27961 tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg gtaaccccgg
    28021 gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc tgccggagcg
    28081 cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta ctatcagccg
    28141 cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg ccgaacggct
    28201 gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca gctggctgcc
    28261 tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag cctacgagga
    28321 ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg aggtgatgcc
    28381 ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga aacaaaaagg
    28441 gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg actggacagc
    28501 tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga aatgtgcgca
    28561 tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg gtggaacacg
    28621 gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg ccggtgcgat
    28681 acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata acggttccgg
    28741 tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc tggggattaa
    28801 acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac gcctgaaccg
    28861 gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg gtgctgaccg
    28921 ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc tgcaacagga
    28981 caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt gggcggagct
    29041 tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt ctgagctgcc
    29101 gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac gccaggcaga
    29161 agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt tccgcccaat
    29221 ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt actacgcgcc
    29281 ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc atgacgccga
    29341 gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat ggaacggcaa
    29401 taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc gtattaaagg
    29461 aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta ttcagattct
    29521 ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg gtaatgtggt
    29581 ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac gcgatttccg
    29641 gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg cctgaataaa
    29701 aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt gaaataaccg
    29761 aggaaatact atgactgata ttgatga
//
LOCUS       DAJTUM010000044        29555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTUM010000044 DAJTUM010000000
VERSION     DAJTUM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..441)
                     /locus_tag="OO471_002101"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO471_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO471_002102"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO471_002102"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6243372.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO471_002103"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO471_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6243373.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO471_002104"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO471_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6243374.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO471_002105"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO471_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO471_002106"
     CDS             5754..6257
                     /locus_tag="OO471_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6243375.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO471_002107"
     CDS             complement(6337..6588)
                     /locus_tag="OO471_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6243376.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO471_002108"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO471_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6243377.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO471_002109"
     CDS             7052..7375
                     /locus_tag="OO471_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6243378.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO471_002110"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO471_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6243379.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO471_002111"
     CDS             complement(8080..8319)
                     /locus_tag="OO471_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6243380.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO471_002112"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO471_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6243381.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO471_002113"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO471_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6243382.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO471_002114"
     tRNA            complement(10643..10729)
                     /locus_tag="OO471_002114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO471_002115"
     tRNA            complement(10742..10815)
                     /locus_tag="OO471_002115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO471_002116"
     tRNA            complement(10870..10945)
                     /locus_tag="OO471_002116"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO471_002117"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO471_002117"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6243383.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO471_002118"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO471_002118"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6243384.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO471_002119"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO471_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6243385.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO471_002120"
     CDS             14483..14659
                     /locus_tag="OO471_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243386.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO471_002121"
     CDS             14646..14870
                     /locus_tag="OO471_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6243387.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO471_002122"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO471_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO471_002123"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO471_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6243388.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO471_002124"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO471_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6243389.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO471_002125"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO471_002125"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6243390.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO471_002126"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO471_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6243391.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO471_002127"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO471_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6243392.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO471_002128"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO471_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO471_002129"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO471_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6243393.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO471_002130"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO471_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6243394.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO471_002131"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO471_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6243395.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO471_002132"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO471_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6243396.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO471_002133"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO471_002133"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6243397.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO471_002134"
     CDS             complement(26368..26781)
                     /locus_tag="OO471_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6243398.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO471_002135"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO471_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6243399.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO471_002136"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO471_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6243400.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO471_002137"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO471_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29555)
                     /locus_tag="OO471_002138"
     CDS             complement(29220..>29555)
                     /locus_tag="OO471_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6243401.1"
                     /translation="KSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCA
                     CCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQE
                     PTEMIQATA"
BASE COUNT         7356 a         7635 c         7035 g         7529 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcaactcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattt
//
LOCUS       DAJTUM010000045        29308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTUM010000045 DAJTUM010000000
VERSION     DAJTUM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            159..1236
                     /locus_tag="OO471_002139"
                     /pseudo
     CDS             159..1236
                     /locus_tag="OO471_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1629..1970)
                     /locus_tag="OO471_002140"
     CDS             complement(1629..1970)
                     /locus_tag="OO471_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6243402.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1983..2855)
                     /gene="copD"
                     /locus_tag="OO471_002141"
                     /pseudo
     CDS             complement(1983..2855)
                     /gene="copD"
                     /locus_tag="OO471_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(2859..3233)
                     /gene="yobA"
                     /locus_tag="OO471_002142"
     CDS             complement(2859..3233)
                     /gene="yobA"
                     /locus_tag="OO471_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6243403.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3372..3602
                     /gene="holE"
                     /locus_tag="OO471_002143"
     CDS             3372..3602
                     /gene="holE"
                     /locus_tag="OO471_002143"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6243404.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3704..4360
                     /locus_tag="OO471_002144"
                     /pseudo
     CDS             3704..4360
                     /locus_tag="OO471_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4384..5046
                     /gene="exoX"
                     /locus_tag="OO471_002145"
     CDS             4384..5046
                     /gene="exoX"
                     /locus_tag="OO471_002145"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6243405.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5043..7103)
                     /gene="ptrB"
                     /locus_tag="OO471_002146"
     CDS             complement(5043..7103)
                     /gene="ptrB"
                     /locus_tag="OO471_002146"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6243406.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7313..7972)
                     /locus_tag="OO471_002147"
     CDS             complement(7313..7972)
                     /locus_tag="OO471_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6243407.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8299..8655)
                     /locus_tag="OO471_002148"
     CDS             complement(8299..8655)
                     /locus_tag="OO471_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6243408.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8722..9012)
                     /gene="yebG"
                     /locus_tag="OO471_002149"
                     /pseudo
     CDS             complement(8722..9012)
                     /gene="yebG"
                     /locus_tag="OO471_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9146..10324
                     /gene="purT"
                     /locus_tag="OO471_002150"
     CDS             9146..10324
                     /gene="purT"
                     /locus_tag="OO471_002150"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6243409.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10380..11021)
                     /gene="kdgA"
                     /locus_tag="OO471_002151"
                     /gene_synonym="eda"
     CDS             complement(10380..11021)
                     /gene="kdgA"
                     /locus_tag="OO471_002151"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6243410.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11058..12869)
                     /gene="edd"
                     /locus_tag="OO471_002152"
     CDS             complement(11058..12869)
                     /gene="edd"
                     /locus_tag="OO471_002152"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6243411.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13104..14579)
                     /gene="zwf"
                     /locus_tag="OO471_002153"
     CDS             complement(13104..14579)
                     /gene="zwf"
                     /locus_tag="OO471_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6243412.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14917..15786
                     /gene="hexR"
                     /locus_tag="OO471_002154"
                     /gene_synonym="yebK"
     CDS             14917..15786
                     /gene="hexR"
                     /locus_tag="OO471_002154"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6243413.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15914..17356
                     /gene="pyk"
                     /locus_tag="OO471_002155"
     CDS             15914..17356
                     /gene="pyk"
                     /locus_tag="OO471_002155"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6243414.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17487..18458)
                     /gene="lpxM"
                     /locus_tag="OO471_002156"
                     /gene_synonym="msbB"
     CDS             complement(17487..18458)
                     /gene="lpxM"
                     /locus_tag="OO471_002156"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6243415.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18578..19900)
                     /gene="mepM"
                     /locus_tag="OO471_002157"
     CDS             complement(18578..19900)
                     /gene="mepM"
                     /locus_tag="OO471_002157"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6243416.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(19916..20848)
                     /gene="znuA"
                     /locus_tag="OO471_002158"
     CDS             complement(19916..20848)
                     /gene="znuA"
                     /locus_tag="OO471_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6243417.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            20927..21682
                     /gene="znuC"
                     /locus_tag="OO471_002159"
     CDS             20927..21682
                     /gene="znuC"
                     /locus_tag="OO471_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6243418.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21679..22464
                     /gene="znuB"
                     /locus_tag="OO471_002160"
     CDS             21679..22464
                     /gene="znuB"
                     /locus_tag="OO471_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6243419.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22611..23621)
                     /gene="ruvB"
                     /locus_tag="OO471_002161"
     CDS             complement(22611..23621)
                     /gene="ruvB"
                     /locus_tag="OO471_002161"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6243420.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23630..24241)
                     /gene="ruvA"
                     /locus_tag="OO471_002162"
     CDS             complement(23630..24241)
                     /gene="ruvA"
                     /locus_tag="OO471_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6243421.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24380..24445)
                     /gene="yobI"
                     /locus_tag="OO471_002163"
     CDS             complement(24380..24445)
                     /gene="yobI"
                     /locus_tag="OO471_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6243422.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24515..25117
                     /locus_tag="OO471_002164"
     CDS             24515..25117
                     /locus_tag="OO471_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6243423.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25119..25640)
                     /gene="ruvC"
                     /locus_tag="OO471_002165"
     CDS             complement(25119..25640)
                     /gene="ruvC"
                     /locus_tag="OO471_002165"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6243424.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25675..26415)
                     /locus_tag="OO471_002166"
     CDS             complement(25675..26415)
                     /locus_tag="OO471_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6243425.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26444..26896)
                     /gene="nudB"
                     /locus_tag="OO471_002167"
     CDS             complement(26444..26896)
                     /gene="nudB"
                     /locus_tag="OO471_002167"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6243426.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27014..28786)
                     /gene="aspS"
                     /locus_tag="OO471_002168"
     CDS             complement(27014..28786)
                     /gene="aspS"
                     /locus_tag="OO471_002168"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6243427.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29096..>29308
                     /locus_tag="OO471_002169"
     CDS             29096..>29308
                     /locus_tag="OO471_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6243428.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7171 a         7533 c         7203 g         7401 t
ORIGIN      
        1 tggaaacatt tatcctgcac ctgaactaca cgggaggagt tacagggtgg ttccggaggc
       61 tttctatatc aacccaaata aggttgatac cgatataaca caccatcagc ctaatgggcg
      121 acaagggaga gacagtccgt taatggagaa gttaaaacat gcagcggaaa aaatacgatc
      181 ccaatttgcc tagaaactta acatatcgaa ggagagacaa agcatattac tggcgcaacc
      241 ctctgacgaa agaagaattt acactaggta aaatttcaag aagagatgca gtagcgcagg
      301 caattgaagc aaatcattat atatacaaaa actactctcc tgctgcccta attgaaaagc
      361 ttaaaggggt cgactcattt actatggcag gctggattga acgttacaaa acgattctta
      421 taaggagaaa agcgtccaga aatacttata aaactcgggt aaatcaactg gagacaataa
      481 aagaaaaatt aggaggggtt ttactgacag aaataaccac tcgccatatc gccgagtttc
      541 ttgatttgta gattgaagga gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat
      601 ctgatatgtt ccgcgaagcc attgttgaag gacgtatatc tcaaaatcca gtaacgccaa
      661 caagagcacc gaaaatagta gttacaagag aacggctgaa actaaagaca tacaactgca
      721 tcagggaggc agcagatcaa cttccggcat ggttcccatt agctatggac ttagcccttg
      781 taacaggaca acgtcgcgaa gacataacga atatgcggtt cagtgatatt tatgatgatc
      841 gtctccacat caggcaaatt aagacaggaa tgatgattgc tatccccctg tcactcagcc
      901 ttcctgtcgc tggtttacga cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg
      961 gggattatct aatcagtgcc gggattagaa aaaacagccc tgacggcagc attcacccgg
     1021 acggcctgac aaagaaattt gtcgcagcca gaaaattaac aggtatcact gttcagtgaa
     1081 aacccaccaa cttttcacga aatcagaagc ctggctggac gattgtacaa agaaatatgt
     1141 ggagaagaat ttgctcagcg tctacttggc cacacatcgg agaagacaac aaaaatgtat
     1201 cttgatgaga gagaaaaaac gtacttactg ctctgatttt aacgtaaatg gattgttaaa
     1261 tgtattttgg ttgtgatata accaaaaaag accggaatac agaaattcga gtaaatttcg
     1321 gggaatttcg gggagacgtt tgcaactaat tgattttaaa tacaattaaa aaaagaccga
     1381 atacgattcc tgtattcggt ccagggaaat ggctcttggg agagagccgt gcgctaaaag
     1441 ttggcattaa tgcaggctta gttgccttgc cctttaagaa tagatgacga cgccaggttt
     1501 tccagtttgc gtgcaaaatg gtcaataaaa agcgcggtgc tcatcagctt aaatgttaaa
     1561 aaccgcccgt tctggtgaaa gaactgaggc ggttttttta ttggaaatca aaaggctatt
     1621 ttaggtaatt aacagagttt ttcagctcgt tctataaacg gtgccagact cattttttcg
     1681 ccgggattgt taggatcatc aatctgaatc accgaaatgg gttgggcatt ggtcttccca
     1741 ctggcaactt ccttttgtgc gatatcgttt aaaggatact gcacgagggt acttgggttg
     1801 atgacataca aagcattacc cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc
     1861 catttgtctt tacccacttc aaaacgactc acggttatca cctgtggagc cgccagcgcc
     1921 gctgcagaac tggtgagtaa cagaaacgcc agaatacttt ttttcatcat tttcgtaatc
     1981 catcaaaaag gttcgagagt cgcaagcagg ctgatgatca ccagcactac cgcacctatc
     2041 gcccattcga gtttagtcat ccacacaaac cagggtgccg cacgatcttc atcctgcctc
     2101 atgcgcggta caagaacata acgattagcc agcgcaatga ccaccataat catcaccagg
     2161 atggctttga gcaataagag ttggccccag taagtcgtaa gcgtgggggg aaatccggta
     2221 atcaacaatg cattaagcac gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac
     2281 caggaaaaac gcatcagcgc ctgaatagcc tagtgtcgcc aacggccttt gatgagttgc
     2341 atacaccaga gcaccggcag taatccccca aaccacgccg ccgcacaaat caggtgaatc
     2401 gcgtgattag tctgatggat tttcgctgtt accccttcat tcagcgtcgc ataccccact
     2461 cccgccagca gaataaattg cgcggtagtg agcataaaca gcaaacgtgg catattccgc
     2521 ggttgcataa gggcgacgat caacgttacg agggcgagaa caatttgcca taaccagacg
     2581 ccaccaaact gcgtttgcaa aacagcctgc caaatgtttg gcgaaaatac atctgtccat
     2641 cccgttccca tcaacccgcc ctgaacggcg agcattgcag tggcactgat caaactccag
     2701 acggcagcgt gctgttgtaa acgcagagaa cgcttcgtca gcaagcgacg aatcattaag
     2761 ggggccagcc aggcaccata cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg
     2821 ataaatcgca gcgcgatcca ggtaaacgcc agcatgattt atttcacgct aaaggtgtag
     2881 tgtcctttcg ttttatgccc gtccaccgaa acaacatgcc agtcaacggt gtaggttccg
     2941 ggtttcagcg aatcggccag tggaacaatc agttgctttt ggtcctgctc attccgcttc
     3001 gctggtaatg ttttaatatt ttcgtttttt ggccccgtga tttttgcacc actgaatcct
     3061 gtttcaacgc cttccgagaa gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg
     3121 tttgccgcag gatactgatg cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac
     3181 gaagtggtca ggattgccag cgcgtagcga agggagcgtg cagttgaagc catattatct
     3241 attccttttt gtaataactt ttttacagag cataaccttg tctaatgtct gagtcgagga
     3301 tcatcaattc cggcttgcca tccaggctca ctcttagtaa cttttgcccg cgaatgatga
     3361 ggagattaag aatgctgaag aatctggcta aactggatca aacagaaatg gataaagtga
     3421 atgtcgattt ggcggcggcc ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg
     3481 ctgaagcggt tgaacgtgaa cagccagaac atttgcgcag ctggtttcgc gagcggctta
     3541 ttgcccaccg tttggcttcg gtcaatctgt cacgtttacc ttacgagccc aaacttaaat
     3601 aaaacttata cagagttaca ctttcttaca taacgcctgc taaattatga gtgttttcta
     3661 aaccgccccc ataatttgca gtcattttga aaaggaagtc attatgtcgt tctggaaagt
     3721 tgcagcagcg caatatgagc ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct
     3781 ggagtttgtg agggccgccg ccaggcaaca atgtcagctg ttagtttttc catcgctttc
     3841 tttactgggg tgtgattatt cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt
     3901 agacccgctt tgttatgccg caacgaccta gcgaatgacc atcattgccg gccttcccgt
     3961 tgaatataac gatcgcttta ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc
     4021 tgggaactac catcagagtc atggtgcatg tctgggccgt cgttccagaa ccatcacggt
     4081 ggtcgatgaa caaccgcaag gtatggatat ggacccgacc tgttcactgt ttacgacagg
     4141 gcaatgtctg ggagaacccg acctgctggc gtcggcccgt cgcttacaat ttttttcaca
     4201 tcagtactcg attgccgtgc tgatggcaaa tgcccgtggt aacagtgcac tatgggatga
     4261 atatggtcgt ctcatcgttc gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc
     4321 atcacagggt tggcaaggcg atatcattcc attacgctag gctttttcgg cctggatcat
     4381 gccatgttgc gcattatcga tacagaaacc tgcggtttgc agggagggat cgttgagatt
     4441 gcctctgttg atgtcattga cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc
     4501 gatcgtccta ttagtccaca agcgatggcg attcatcgca tcaccgaagc catggtcgcc
     4561 gataaaccgt ggattgaaga tgtgatccca cactattacg gtagtgaatg gtatgtcgcg
     4621 cacaacgcca gctttgaccg ccgcgtactg cctgagatgc ccggtgagtg gatttgcact
     4681 atgaaactgg cccgtcgttt gtggccaggg ctcaagtaca gcaatatggc gttatataaa
     4741 acacgcaagc tcaatgtaca gacaccgctg ggcctgcatc atcaccgcgc gttgtatgac
     4801 tgttatatca ccgccgcgtt gcttatcgat attatgaaca cctccggctg gacggcagaa
     4861 cagatggccg atatcaccgg acgtccgtcg ttgatgacaa ccttcacctt tggcaaatac
     4921 cgtggcaaag cggtttccga cgttgccgaa cgctatccgg gctatctgcg ctggttattt
     4981 aataacctgg acagcatgag cccggagctg cgtttaacac tgaaacatta tctggaaaat
     5041 acttaatccg caggcgtagc gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat
     5101 tccatcgcta cgccttcgta cgatttaaag cggccagatt taccgccatg gcctgagtcc
     5161 atgtcggtac agagcaataa aagatggtca tcggttttca gctcgcgcaa tttagcgacc
     5221 cattttgccg gttcccaata ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa
     5281 tgcggataag cctgtgcggt gacgttgtca tacgggctgt agcttttcat gtactcgtaa
     5341 tattgcggat cctgcggatt tccccactct tcaaactcac cagtggtaag aggaattgat
     5401 tcatcaagca tcgttgttac aacatcaaca aacggtacct gggcgataac gccgtggaat
     5461 aattccgggc gttgattaat tgcaacgccc atcaacatgc cccccgcact tccgcccatc
     5521 gcataacaga gcgaaggaga gccatagccc agttttaaca atgcatcgca ggcatcaaga
     5581 taatcattaa acgtattttt cttcttcaga aatttaccgt cttcgtacca ttgttgcccc
     5641 agctcaccac cgccgcgaac atggacaatg gcgtagacaa agccacgatc taacaaactc
     5701 aagcggctaa aactgaaatc ggcatcaata cttgcgccgt aagaaccata gccatacacc
     5761 agcaacgggt tgtgtccttt gcgaaaatgt ttgcgatgat agaccagcga aaccggaact
     5821 tcgacgccat cacgggcgac tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa
     5881 ccaggcactt ctgtttgttt taatacacga cgctcaccgg tatccatatc cagttcaaac
     5941 aaagtgtctg gtgtggtcat ggaagaataa ccataacgca atcgcgcggt ttcaggttct
     6001 ggattgtagg caatccaggt cacataggcc ggatcatcaa aggcaatgcc aatgacttcc
     6061 cgggtcttgc ggttaatttg gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc
     6121 agccagtcgg taaacagcgt aaacccttcc agcatgatgt tttcgcgtgg cggaattaac
     6181 tcttcccact gttgctcatc acgcatacgg gtacggtata agccaaagtt tttgccgtgg
     6241 cgattggaac gcagataaaa acgatgctgg tagtgatcaa ggctgtattc gtgatctttg
     6301 cggcgcggca gaaaaacaaa cggctcggca tcggccattt ccgcgtccag caggcgaact
     6361 tcactggtgg tggcgctggc cagatgaatg acgacatagt gcttcgacgt cgttttatgc
     6421 aggctgacat aataggtatc gtctttttct tcgtagatca gtgtatcttg cgatgctggc
     6481 gtaccgatgg cgtgacgcca gacctgataa ggcagcagcg tcaccggatg cttgcgaaca
     6541 tagtagaaag tccaggagtc atttgcccag acaaagctgg gttcaacgtt atccagcagt
     6601 tccgggtacc agttacctgt ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa
     6661 agaaaatctt ctgccagcgc cataatggtg ttatcgggcg taatcgccat tccgcccatc
     6721 gaataaaact cactatgagc tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc
     6781 cactcttcgc tgaatgccga ttgacgctgg tagatagcat attcacagcc tggttcataa
     6841 atatgccgat agcggtagcc attttttatg tagggcgcag aaacttctct ttgcggaatg
     6901 cggtcgatga tttcctttaa gatgcgatcc tgcaaggctt gttgtgaggc catcacccga
     6961 tgaccgtaac tattttcttg ttgcaggtag tccaggactt ttggctgaga acgcgtatcg
     7021 tcccgcagcc agtagtaatt atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga
     7081 atgcgggcgg cttttggtag catgttattg ttctttctgg ttggaaacat cttataaggg
     7141 tggcaaaact caccggggat gcaagcgaaa cagggtcgtc attgcttaga tgatgacagg
     7201 taatggcgcg gatatcgaat gttatgcaga cagagaaaat cagcctgttc aaatggctgt
     7261 gcggttctgg atagcccgaa atagtcaact tcaggctatc cagagagcgg aattattccg
     7321 ccaaagtgcg tttttgctgt tcgagatcgc gttcaatgcc ttcacgaaca tcctggggga
     7381 ttttcagcgc gtcacccagt gcattcaggt aactgcgttc cataaaatgg tcaatatcaa
     7441 tagccgcgca gctcaggaaa tagatttcca gcgcctcttc ttcattgcgg accccggtag
     7501 ccaggcgttg tggatccagc ggttgttcga ttgcctgctc aatgagtaca cgcccctgct
     7561 cttccacgcc ggcttcacgc aattgctggt cgattgccgc acgttcattg gcatcaatat
     7621 gaccatcact tttagcggca aagaccagcg caaggatcaa acgttccgta cgctcatcca
     7681 gcggcgtact ttgcgcgcca aactgcggtt cgtcctgatg cgccgcgcga attttatctt
     7741 tgtatttatt ccacagcacc gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg
     7801 tgccatattt cgtaagaagt ttacgtgctg atttatttgc gaccagcagc ccagccagtc
     7861 cgcctaatgc gcctgacact aacagtttgc ccaatccctg atccgcagac gaggaggtag
     7921 aagaactgct ttgcccaagc agggattgca gttgatttaa ccagttagcc atatttgctc
     7981 ctcaataacc atttttagtg cctgtagcat agcggatgag gatgtcagaa aatgtatatc
     8041 gagacaaaag atgcgcaaat aagcggggcg gtaggccgtt attcaaagaa attcgcgcca
     8101 ggtaaggtat ttactgtata tcaggtgata agggtatttt aggtgaaagt agtttgtcgt
     8161 tttgagtggc attgtttgat gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc
     8221 tacgattgtg gtgcgttctg ggacggacaa ggcactcgcg ccgcatccgc caacggtcaa
     8281 tcgaattatc agaagccatt aacgccgctg atattccgcc attcccgctt tgcagtccac
     8341 gcagacctgg taatgaatat cggcactttt accacgcacg gttagcggta ctgaccattt
     8401 atcatcttta ccctgaatgt cctgcaaact gacccaggcc acgggatcgg cctgaccgac
     8461 aattttttga tcatctgccc agcgcgccac gcgattttgt tgataatcac gttttacgct
     8521 cgcggcaatt ccggcagcat ccagaccttc acactttggg aaagtgaccg acttgctggt
     8581 ttcattattg gcagcgaaaa ctgatgcgca ggcagaaacc aacaaccgcc ctaaaaacgc
     8641 ccctcttttt ttcatgtttt tctccatagc acaatgattc aggagaaagc atggtacaaa
     8701 ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt cggattcagc
     8761 accgacgacc tgtggggacg gtaatttgcc ggttttcaga atggtgctca gcacctcttt
     8821 ttgttctgcc agccatagcg aaagggcttc acgttgctcg tcttccattt gcactggaga
     8881 atttgtcagc taggtgtcaa gcagatccgc cgtatcaagc attttgtcat aagcatcggc
     8941 ttcctttttg ctggtaaacg acattttctc ttcgccctca cgaatgacta cgtatttaac
     9001 ttcaaccgcc atttgcagcc tctcataata actgagattt tatacagtat atttcttttc
     9061 ggttgagaaa tcaacatcag caataaagac acacgcaaac gttttcgttt atactgcgcg
     9121 cggaattaat caggggatat tcgttatgac gttattaggc actgcgctgc gtccggcagc
     9181 aactcgcgtg atgttattag gctccggtga actgggtaaa gaagtggcaa tcgagtgtca
     9241 gcgtctcggc gtagaggtga ttgccgtcga tcgctatgcc gacgcaccag ccatgcatgt
     9301 cgcgcatcgc tcccatgtca ttaatatgct tgatggtgat gcattacgcc gtgtggttga
     9361 actggaaaaa ccacattata tcgtgccgga gatcgaagct attgccaccg atatgctgat
     9421 ccaacttgaa gaggaaggac tgaatgttgt cccctgcgct cgcgcaacga aattaacgat
     9481 gaatcgcgag ggtatccgtc gcctggcggc agaagagctg cagctgccca cttccactta
     9541 tcgttttgcc gatagcaaaa gccttttccg cgaggcggtt gccgccattg gctatccctg
     9601 cattgtaaaa ccggtgatga gctcttccgg caaggggcag acgtttattc gctctgcaga
     9661 gcaacttgct caggcatggg aatacgctca gcaaggcggt cgcgccggag cgggccgcgt
     9721 aattgttgaa ggcgtcgtta agtttgactt cgaaattacc ctgctaaccg tcagcgcggt
     9781 ggatggcgtc catttctgtg caccagtagg tcatcgccag gaagatggcg actaccgtga
     9841 atcctggcaa ccacagcaaa tgagcccgct tgcccttgaa cgtgcgcagg agattgcccg
     9901 taaagtggtg ctggcactgg gcggttatgg tctgtttggt gtcgagctgt ttgtctgtgg
     9961 tgatgaggtg attttcagtg aggtctcccc tcgtccacat gataccggga tggtgacgtt
    10021 aatttctcaa gatctctcag agtttgccct gcatgtacgt gccttcctcg gacttcctgt
    10081 tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt attctgccac aactgaccag
    10141 tcagaatgtc acgtttgata atgtgcagaa tgccgtaggc gcagatttgc agattcgttt
    10201 atttggtaag ccggaaattg atggcagccg tcatctgggg gtggtactgg ctacttcaga
    10261 gagtgttgtt gacgccattg aacgcgcgaa gcacgccgcc ggacaggtaa aagtacaggg
    10321 ttaaacccgg caaaaaaacg ctacaaaaat gcccgatcct agatcgggca ttttgacttt
    10381 tacagcttag cgccttctac agcttcacgc gccagcttag taatgcggtc gtaatcgccc
    10441 gcttccagcg catctgccgg aaccagccag gaaccaccga tgcacagcac gcttttcagt
    10501 gccaggtagt cacggtagtt agccggagaa ataccacccg tcgggcagaa acggacctgg
    10561 gagaacggac ccgcgatcgc ctgcagggct ttcacgccgc cgttagcttc agccgggaag
    10621 aatttgaact ctttcaaacc gtagtccata cccagcatca gttcggaaac agtgctgatc
    10681 cccggaatca gaggaatagt cccttcggta gcagctttca gcagcggctc ggtcagaccc
    10741 gggctaattg cgaactgtgc acccgcttca gtgacttctg ccagctgctg tggattcagc
    10801 accgtaccgg cacccacaat cgcttcaggc acttctttgg cgatagcacg gatagcgtca
    10861 actgcacact cggtacgcag agtcacttcc agaacgcgca ccccaccagc aaccaacgct
    10921 tttgccatcg gcaccgcgtg ttccagtttt tttaccacga taaccggtac aaccgggccg
    10981 gtggtcagga ttgattctgc acttgttttc cagtttttca tcagagtttt ctctcgcctg
    11041 attacaaatt tgtcgtctta aaaagtgata caggttgcgc cctgttcggc accggacagt
    11101 ttttcacgca aggcgctgaa taattcacgt cctgttccca cgcgtgacgc gctcaggtca
    11161 ggaatgtgcg gttcgcgagc agccagttcc gcttcgtcta ccagcagtgt cagttcgcct
    11221 gtctgtccat tcacacgaat gatgtccccg tcgcgcactt ttgccagcag cccgccatcg
    11281 taggcttctg gtgttacgtg gatagctgac ggcactttac ctgaagcgcc ggagagtcgt
    11341 ccatcggtaa ctaacgcaat tttgaaacac cggtccaata atacaccaag tggcggcatg
    11401 agtttatgta attctggcat tccgttcgct tttggcccct gatgacggac aacaacgaca
    11461 caatcgcggt ccagcaaacc cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa
    11521 acaaccgctg gcgcttcaat cacctggttc tcaaccggca cggcagaggt tttcataacc
    11581 gcacggccca ggttaccgct taacactttt gtcccaccat gatgagagaa aggttgttcg
    11641 aaggaagcga tcacattgct gtcgagtgat ttttccgccc cttcccgcca gtccagttca
    11701 ccattattca gccatggttc aagggtataa cgagacagac caaagcctgc caccgtattg
    11761 acatcttcat gcagcaggcc tgctttgagc agttcacgca ccagaaccgg tacgccacct
    11821 gccgcctgga agtggttaat atcggccgga ccgttcgggt agagacgtgc catcagcggt
    11881 acaacatcag aaaggtcaga gaagtcatcc cagttaatct gaataccggc cgcgcgcgcc
    11941 atcgccacca ggtgcatggt gtggttagtg gaaccaccgg tcgccagcag tgcaacgata
    12001 ccgttcacca ccactttctc atcgatcatc ttaccgatcg gcatccattc attaccatta
    12061 ccggtcatgc gtgtaacctg acgcgcagcg gcagcggtca aagcatcgcg cagcggagaa
    12121 tccggatgaa caaaagaaga gcctggcaac tgcatcccca taaattccac caccatctgg
    12181 ttggtgttgg cagtaccgta gaaagtacat gttcccggcg catggtaaga cgcggcttct
    12241 gactccagta aggccatgcg gtccacttta ccttcggcat aaagctggcg aatacgcact
    12301 ttttctttat ttggcaaacc gcttgccatc ggtccagacg gcacaaacac cgcaggcaaa
    12361 tgaccaaacg acagggctgc catcgtcaga cccgggacaa tcttgtcgca cacaccgagg
    12421 aacagagcac catcaaacat gttatgggac agccccaccg ccgcagacat cgctatcact
    12481 tcgcggctta gcagcgacaa ttccattcca tcctgcccct gggtgacacc atcacacatc
    12541 gccggaacac cgcccgcaac ctgaccaacc gcattcgctt catgcagggc tttacgaatg
    12601 atttctggat agtgttcata aggctggtgc gcggagagca tgtcgttata ggaggtgatg
    12661 atggcgatat tgttacgcaa catgcttttc aaagaggctt tgttttctgg ctggcaggca
    12721 gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac gatgaacggt cgacgttttc
    12781 gcttgttcta tccgggcgag ataagcagag cgagtctcgc gcgaacgttc aatgattcga
    12841 tttgttacgc gtaacaattg tggattcata aaggctcctg aaattgagtt gtcagagcag
    12901 gatgattcac aacgcgtttc attcagagga tttatgagtg aaacgcctgt aaccggagct
    12961 catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg
    13021 aggcctgaaa gtgtaaaaat tgttctacaa tctgtgcaag atcatgttac cggtaaaata
    13081 accataaagg ataagcgctg atattactca aactcattcc aggaacgacc atcacgggta
    13141 atcatcgcca ccgaggcaac gggtccccag gttccggcct gatacggttt cggcgcatca
    13201 ttgtccatcg cccacgcctc agtaatggag tctacccatt tccaggcttc ttccacttcg
    13261 tcgcgacgta caaacagtgc ctgaatacca cgcatggttt ccagcagcaa acgttcatag
    13321 gcatccgcca gatgcgtctg attaaaggtt tctgaatagc tcagatccag cttggtgatt
    13381 tgcaggttat gtttgtggtc aaggccagga actttattca gtacctggat atccacgcct
    13441 tcatcaggtt gcagacggat agtcagttta ttctgcggca gatcctgcca cgactcttta
    13501 aacagattca gttcaggtgt tttgaaatag accacgactt cagaacattt ggtcggcaga
    13561 cgtttaccag tacgcaggta gaatggcaca ccggcccagc gccagttatc aatgtcgacg
    13621 cggatcgcca cgaaggtttc tgtattgctg cttttgttcg cgccctcttc ttccagatat
    13681 cccggcactt ttttgccctg ggcgaagccc gcagtatatt gcccgcgtac ggttttttcg
    13741 cgtacgttgg agcggtcgat gcggcgcagg gacttcagta ctttcacttt ttcatcgcgg
    13801 atgctgtctg cgctcaggtc agatggcgga gacatcgcaa tcatgcagag aatttgtagc
    13861 aggtggttct gaatcatgtc acgcatctga ccggctttat caaaatagcc ccagcgccct
    13921 tcgatcccca cttcttctgc cacggtaatc tcaacatgat caatggtgcg attgtcccag
    13981 ttattcacaa acagggagtt agcaaaacgc agcgccaaca ggttcagcac cgtttcttta
    14041 ccaagatagt ggtcgatacg gtaaacctgg cactcctcga agtattcgcc aacctgatca
    14101 ttgatttcct gcgaggtcgc cagcgacgtc cccagcggtt tctccatgac tacgcgtgcc
    14161 ggtttagcat tcagttttgc ctcgccaagc cctttgcaaa ttgcgccaaa agtgctgggc
    14221 ggcatggcaa agtagttaat agtgatacga tttttttgat ccagcatcgc gccgagacgg
    14281 ttgaatgcag cagtgtcatt gacatcgaga ttacaaaaat ccagacgtgc actcagggtg
    14341 tcccataaac cttcatcaat ggtttctttc atgaaagttt cgagcgcctc gcggacaact
    14401 ttggtatatg ccgctttatc ccagtcagca cgccctacgc cgataatccg ggtgtccggg
    14461 ttgagctgac cgactttttc cagttgatac agggaaggca gcaatttacg acgcgcaagg
    14521 tcgcctttcg cgccgaaaat gaccaggtca caggcctggg ctgtttgcgt taccgccatg
    14581 tcattctcct taagttaact aacccggtac ttaagccagg gtatacttgt aattttctta
    14641 cggtgcactg tactgctttt acgagcttgc gaaaactgta aacgcttatc cacccgtgcg
    14701 attacgggaa aagcgcgcaa agcgcggcaa aactgataaa aaaatcatcg ttttccattc
    14761 ggtaaaaatc tgacactgat catgttatga aaaaaaataa caactttttt atctgctttt
    14821 gtcattaaca cggcacacag gcgtaatatc tgacaaaact gcatttcgat ttctttcagt
    14881 gcggaaatcg tcattacccg tgagtctctt tacatcatga atatgctgga aaaaatccag
    14941 tctcagctgg aacatttgag caaatcagag cgcaaagttg ccgaggtcat tctggcttcg
    15001 cccgataacg cgatccattc gagtattgct gctctggcgc tggaagccaa tgttagcgaa
    15061 ccgacggtga atcgtttctg tcgcagcatg gacacgcgcg gttttcctga ttttaaactt
    15121 catctggcac aaagtctggc gaatggcact ccctatgtta atcgcaacgt caatgaagat
    15181 gacagcgttg aatcatacac ggggaaaata tttgagtccg caatggcaac gctcgatcat
    15241 gtccgtcatt cactggataa atctgccatc aaccgcgccg tcgacttgct cactcaggca
    15301 aaaaaaatcg cctttttcgg attaggctct tctgccgccg ttgcccacga tgcgatgaat
    15361 aagttctttc gttttaatgt tccggtggtg tactccgatg atatcgtgct gcaacgcatg
    15421 agttgtatga attgtagcga cggagacgtg gtggtgctga tttctcacac aggaagaaca
    15481 aaaaatctgg tcgagctggc gcagctggca cgcgaaaacg acgccatggt gattgccctc
    15541 acctctgcgg gtaccccgct cgcccgggaa gcaacgctgg caattaccct cgacgtaccg
    15601 gaagatactg acatttatat gcccatggtt tctcgacttg cacagctgac cgtgatagat
    15661 gtgctggcga caggatttac tttgcgacgc ggtgcaaaat tcagagataa cttgaagcgg
    15721 gtcaaggaag cgctgaagga atcgcgtttt gataagcagt tacttaattt aagtgacgat
    15781 cgctaaaagc gactgtcact gtcctaatct tatacgacat ccgaatgaga ttaatttatc
    15841 gccatcgcgg cgttatttca ttcggatttc atgttcaagc aacacctggt tgtttcagtc
    15901 aacggagtat tacatgtcca aaaggcttcg cagaacaaaa atcgttacca cgttaggccc
    15961 agcaacagat cgcgataata atcttgaaaa agttatcgcg gcgggtgcca acgttgtacg
    16021 tatgaacttt tctcacggct cgcctgaaga tcacaaaatg cgcgcggata aagttcgtga
    16081 gattgccgca aaactggggc gtcatgtggc tattctgggt gacctccagg ggcccaaaat
    16141 ccgtgtatcc acctttaaag aaggcaaagt tttcctcaat attggggata aattcctgct
    16201 cgacgccaac ctgggtaaag gtgaaggcga caaagaaaaa gtcggtatcg actacaaagg
    16261 cctgcctact gacgtcgtgc ctggtgacat cctgctgctg gacgatggtc gcgtccagtt
    16321 aaaagtactg gaagttcagg gcatgaaagt gttcaccgaa gtcaccgtcg gtggtcccct
    16381 ctccaacaat aaaggtatca acaaacttgg cggcggtttg tcggctgaag cgctgaccga
    16441 aaaagacaaa gcagacatta agactgcggc gttgattggc gtagattacc tggctgtctc
    16501 cttcccacgc tgtggcgaag atctgaacta tgcccgtcgc ctggcacgcg atgcaggatg
    16561 tgatgcgaaa attgttgcca aggttgaacg tgcggaagcc gtttgcagcc aggatgcaat
    16621 ggatgacatc atcctcgcct ctgacgtggt aatggttgca cgtggcgacc tcggtgtgga
    16681 aattggcgac ccggaactgg tcggcattca gaaagcgttg atccgtcgtg cgcgtcagct
    16741 aaaccgagcg gtaatcacgg cgacccagat gatggagtca atgattacta acccgatgcc
    16801 gacgcgtgca gaagtcatgg acgtagcaaa cgccgttctg gatggtactg acgctgtgat
    16861 gctgtctgca gaaactgccg ctgggcagta tccgtcagaa actgttgcag ccatggcgcg
    16921 cgtttgcctg ggtgcggaaa aaatcccgag catcaacgtt tctaaacacc gtctggacgt
    16981 tcagttcgac aatgtggaag aagctattgc catgtcggca atgtacgcag ctaaccacct
    17041 gaagggcgtt acggcgatca ttaccatgac cgaatcgggt cgtaccgcgc tgatgacctc
    17101 ccgtatcagc tctggtctgc caattttcgc catgtcgcgc catgaacgta cgctgaacct
    17161 gactgctctc tatcgtggcg ttacgccggt gccctttgat agcgctaatg acggcgtagc
    17221 agctgccagc gaagcggtta atctgctgcg cgataaaggt tacttgatgt ctggtgacct
    17281 ggtgattgtc acccaaggcg acgtgatgag taccgtgggt tctactaata ccacgcgtat
    17341 tttaacggta gagtaagtac gttgccggat gcggcgaaaa cgccacatcc ggcctatagt
    17401 tcaatgatag ttcaacagat ttcgaatatt ctgaagcaaa cttgaactta tcatcaggcg
    17461 aaggcctctc ctcgcgagag gcttttttat ttgatgggat aaagatcttt gcgcttatac
    17521 ggctggattt cgcccggttt gcgagttttc agcaatttta gtatccaggt gtattgttct
    17581 ggtcgcggac caacaaaaat ctcgacttct tcattcatcc gccgctcaat cgtatgatca
    17641 tccgcctcta acagatcatc catcggtggg cgcacctgaa tcgtcagacg atgcgtcttg
    17701 ccatcataaa tcggaaacag cggtacaacg cgcgcacggc atactttcat caaacgacca
    17761 atcgcgggca acgtcgcttt ataggtggca aagaaatcca caaattcgct gtgctctggg
    17821 ccatgatcct gatcgggtaa ataatatccc cagtacccct gacgtaccga ctggatgaat
    17881 ggtttaatac cgtcatttct cgcatgcaga cgaccgccaa agcgacgacg caccgtgttc
    17941 cagacataat caaaaaccgg gttgccctga ttatggaaca tcgctgccat tttctgccct
    18001 tgcgaggcca tcagcatggc aggaatatcg acggcccaac cgtgcggcac cagaaagata
    18061 actttctcgt tattacgccg catctcttcg atgatctcca gcccttgcca gccaacgcgc
    18121 ggctgaattt tctccggccc gcgtattgcc aactcagcca tcattgccat cgcttgcggc
    18181 gcggtggcaa acatctcatc aacaatcgct tcgcgttcag cttcactacg ttctggaaag
    18241 cagagcgaca gattgattaa cgcacgacgg cgtgagcttt ttcccagtcg tccggcaaaa
    18301 cgtcccagcc gtgccagaat gggatcacgg aactttggcg gcgttaaagc gatacccgcc
    18361 atcgctgcta cacccagcca tgctccccag tagcgcgggt ggcgaaagga tttatcaaac
    18421 tcaggaatgt attcgctatt attttttttc gtttccatgc ttttccagtt tcggataagg
    18481 caaaaatcaa tctggtgata gtgtagcggc acaacttgcc ccgcaccaaa taaaaaaagc
    18541 cggtactgac tgcgtaccgg ctgcgaatgg atgttaatta atcaaaccgt agctgcggca
    18601 caatctcttt ggcctgcgcc aggaattcgc gacgatcgga gccggtcagc ccttcggtac
    18661 gcggcagttt tgccgtcagc gggtttacgg cctgctggtt tatccatact tcatagtgca
    18721 gatgcggccc agttgaacgt ccggtattac cggaaagcgc gatacggtcg ccacgtttca
    18781 ccttctgtcc cggtttcacc agaatcttgc gcaagtgcat ataacgcgtg gtgtagctgc
    18841 gaccatgacg aatagccaca taataacctg ctgcgccact gcgtttggca accaccactt
    18901 caccgtcacc cactgaaagc actggcgtac cttgtggcat ggcgaaatca acacctctgt
    18961 gtggtgcaac gcgacctgtc accggattag ttcgacgcgg gttaaagtta gacgagatac
    19021 ggaactgttt cgccgtcggg aatcgcaaga atcctttcgc cagaccagta ccgttacgat
    19081 cgtagaattt gccatcctca gcgcggattg cgtaataatc tttaccttct gaacgcaaac
    19141 gtacgcccag cagctggctt tgctcacgtt taccatcaag catttctcga gacattaata
    19201 ccgcaaattc atcgcctttt ttcagtttgc ggaaatccat ttgccactgc atggctttaa
    19261 tcactgcgct cacttcggcg ctggttaaac cggcgtttct ggcgctggca acaaagctcc
    19321 ctccgacggt acctttcagc agattgttaa cccactctcc ttgctgcatt tcgctggtca
    19381 ttttaaaacc gttagcggca gtacggtcat aggttcgggt ttcacgacga gacacttccc
    19441 aggtgaggcg ctgcagttcg ccgtccgcgg ttaatgtcca ggagagttgt tgaccgattt
    19501 tcaggttacg caattctttg tcggcagcag caagttgggt gatatcaccc atatcaatac
    19561 catactgatt gagaatgctg cttagcgtat cgccagtgga aacaacatat tcatgcacgc
    19621 ccgcttcacc ggcgattttg tcatccagtt cgtcctgggg aatggcttca tcttcttgtg
    19681 cagcttgatc aatcggctca ctggcttcag gtaagagcga acgaatttcg ttctgttcca
    19741 gctcaatggt tttgacaatt ggcgtggcat cacggtgata aacatagggc cgccagacag
    19801 cgacggccag agtaagaacg gtgagcgacc ccaacataac gcggtgtggt cgcggtaaat
    19861 tattaaacgc cagggcgaca gagcgggcta tctgttgcac gtaatcactt cctcattaat
    19921 ctcctttcag gcagctcgca tactggttgg ctaattgact caggaattct gaatagcttg
    19981 ttttacccag tttgatattc gtccccaggg gatccaacgt tcccatacga acggatgtcc
    20041 ctcgtgcgac gctctcaacg accgctggcc tgaactgtgg ctcagcaaaa acgcaggttg
    20101 ctttttgctc aaccaactgt gttcttattt catgtaaacg ctgcgcgcca ggttgaatct
    20161 cagggttaac ggtaaaatga ccaagcggtg tcagtccgaa ctgtttttcg aaatagccgt
    20221 aagcatcgtg aaaaacgaaa taacctttcc ccttgagcgg cgcgagctcg ttaccaacct
    20281 gcgtttcggt tgaggccaat tgtgcctcaa aatccttcag gttggcgtca agtttggctc
    20341 gactttgcgg cataagttcc actaattttc catggattgc aaccgctgta gcccgcgcta
    20401 tctctgggga aagccaaaga tgcatgttga aatcgccgtg atggtgatct tcgtcacttt
    20461 tttccgcgtg gtcgtgatca tcatcatcgc cgtgaatact tttcatcagt agcggtttca
    20521 catcttcaag ctgcgcaatc gttacctgct tcgcttctgg taatttgctt accggttttt
    20581 gcataaacgc ttccatctcc gggccaaccc aaacgactaa gtccgcgttc tgtaagcgtt
    20641 ttacatccga tggacgcagt gaataatcat gttctgaagc gccgtcaggc agtaaaacct
    20701 ccgtttctgt tacaccatca gcaatggcag aagcgatgaa cccaacgggt ttaagcgaag
    20761 cgacaacggc ggcatctgcg gcctgtgttg cacctcccca gagagcggcg gataatgctg
    20821 cgaaaagaag cgttttttta tgtaacataa tgcgaccaat catcgtaatg aatatgagaa
    20881 gtgtgatatt ataacatttc atgactactg caagactaaa attaacatga caagtctggt
    20941 ttccctggaa aatgtctcgg tttcttttgg ccaacgccgc gtcctctctg atgtgtcgct
    21001 ggaacttaaa cctggaaaaa ttttgacttt acttgggcca aatggcgcag gtaagtcgac
    21061 actggtacgg gtagtgctcg ggctggtaac acccgatgaa ggggttatca agcgcaacgg
    21121 aaaactgcgc atcggctatg taccgcagaa gctgtatctc gacaccacgt tgccactgac
    21181 cgtaaaccgt tttttacgct tacgccctgg tacacataaa gaagatattt tgcctgcact
    21241 gaaacgtgtc caggccgggc acctgattaa cgcaccgatg caaaagctct ctggtggcga
    21301 aacgcagcgt gtactattag cgcgagcatt gttaaattgc ccgcaattat tagtgctgga
    21361 tgaacccact caaggcgtgg atgtaaatgg ccaagtggcg ttatatgacc ttattgacca
    21421 gttgcgtcgt gaactggatt gtggcgtttt aatggtttct cacgatctgc atctggtgat
    21481 ggcaaaaacc gatgaagtgc tgtgcctgaa tcaccacatt tgttgttccg gcacaccgga
    21541 agttgtttcc ctgcatccgg agtttatttc aatgtttggt cctcgtggtg ctgaacaact
    21601 gggtatctat cgccatcatc ataatcatcg tcacgattta cagggacgaa ttgttttgcg
    21661 tcggggaaat gatcgctcat gattgaatta ttatttcccg gttggttagc cgggatcatg
    21721 ctcgcctgtg ccgcgggtcc gctgggttcg tttgtagtct ggcgtcgtat gtcttatttc
    21781 ggtgatacgc tggctcatgc ctcattactt ggtgtcgcgt ttggtttgtt gctggacgtg
    21841 aatccattct atgcggtgat tgccgttacg ctgctgctgg cgggcggtct ggtatggctg
    21901 gagaagcgtc cacagctggc gatcgacacg ttattaggga ttatggcgca cagtgccctg
    21961 tcgctgggcc tagtggtcgt tagtctgatg tctaacattc gtgttgattt gatggcttac
    22021 ctgttcggtg atttactggc agtgacgcca gaagatctca tctctattgc gattggcgtg
    22081 gtcatcgtgg tggctatttt gttctggcaa tggcgcaatt tgctgtcgat gacgattagc
    22141 ccggatctgg cgtttgttga tggtgtgaaa ttacagcaag tgaaattgtt gttgatgctg
    22201 gtgacggcat tgacgattgg tgtagcgatg aaatttgtcg gtgcgttgat tattacttcg
    22261 ttgctgatta ttcctgcagc tactgcgcgt cgctttgccc gtacgccgga acagatggct
    22321 ggtgtcgctg ttttggtggg gatggtggca gtaactggcg gtttaacctt ttccgcgttt
    22381 tacgatacgc cggcgggtcc gtcggtggtg ctatgtgcgg cactgttatt tattatcagt
    22441 atgatgaaaa agcaggccag ctaatctatc gctgaacaca tttgtcggat gcggcgcgag
    22501 cgccttatcc gacctacggt tcgctatctc tggctggcct gataagacgc gaacagtgtc
    22561 gcatcagaca cactgccagt gtcggatgcg gcgcgagcga ccaatccgac ttacggcatt
    22621 tctggcggcg ttatgccaaa gtgattccac gcccgcgtcg tcgccatacg cccgcgcggt
    22681 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gtacatcttc aatggtttca
    22741 cgttcttcgc caatggctgc cgccaggtta tccagaccta ccggcccacc aaagaactta
    22801 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcagc atcgacattc
    22861 aacatatcca gcgcctgagc agcgatatct gccgagatgg tgccatcgtg cttcacttcg
    22921 gcgaaatcac gcactcgacg cagcagacgg ttggcaatac gtggcgtacc gcgagcgcga
    22981 cgagctactt cctgcgcacc gtcatcactc atctcaagcc ccataaagcg tgcactgcga
    23041 ctgacgatat attgcagatc cggcacctga taaaactcca gacgttgcac aataccaaaa
    23101 cggtcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcgccaat cagggtaaac
    23161 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    23221 tggtagtctt ccattgccgg gtacagcact tcttcaacaa ctggcgataa acggtggatc
    23281 tcatcaataa acagcacgtc atgcggttca aggttagtga gcatcgcagc caaatcgccc
    23341 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    23401 atgttggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacaa atgatcgagg
    23461 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggc
    23521 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgctac atcttccggc
    23581 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    23641 cgcgtagggc ttcgcgaatt aatgtttcac tgctggtgtc agggcgagcg attttgctca
    23701 ccatgcggct tgcttcttgt ggtttatagc ctagcgccac cagcgcggca actgcttctt
    23761 gttcagcatc gtcggtcgcc gggctagcag gagacgtgag taccaggtcg gcggctggcg
    23821 taaagagatc gccatgcaaa cctttaaatc ggtctttcat ttcaacaatc aagcgttcgg
    23881 cggttttttt gccaataccc ggcagtttca ccagtgcccc cacttcttca cgctcaacgg
    23941 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagggccaac ttcgggccga
    24001 cgccgttggt tttgatcaac tctttgaaca acgtgcgttc ttgtttattg ttaaaaccgt
    24061 acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg
    24121 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    24181 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    24241 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    24301 atatccagcg aaggatgaag aaaacttgcg aggtgtcccg atgatctgaa aaatggcaca
    24361 gtataattta ttcaacaggt tatattggaa gcaaatattt gaatattaca tattcagtga
    24421 agaaatgcgt aataaaaata tacattgcga ctcctgaaaa ataatttttt tatgctatta
    24481 cgtatatttg tatctatttc aatggaatga caacgtgaat attaattacc ctgctgaata
    24541 tgaaattgga gatatcgtct ttacatgtat aggtgctgcc ttatttggtc aaatatcagc
    24601 tgcatcaaat tgctggagta atcacgtcgg gatcattatc ggtcataacg gtgaagactt
    24661 cctggttgca gaaagccggg ttcccctttc aaccatcacc acgctatctc gttttattaa
    24721 acgttcgtct aatcaacgct atgctataaa gcgattagac gctgggctga cagaacaaca
    24781 aaaacaacga attgttgaac aggttccctc ccggctacgc aaactttacc acacgggttt
    24841 taaatacgaa tcttcgcgcc agttctgttc aaaatttgtc tttgatattt ataaagaggc
    24901 gctatgtatt ccggtgggtg aaatagagac gtttggacaa ctgttaaata gcaatccgaa
    24961 tgcaaaactc actttctgga aattctggtt cttaggttct attccgtggg agcgtaaaac
    25021 cgtcacgcca gccagtttgt ggcatcatcc gggtttggtg ttgattcacg cggagggagt
    25081 tgaaacgcct cagccggaac tgaccgaggc ggtataactt aacgcagtcg ccctctcgtc
    25141 aggttcagcc gcgattcgct catctgcatc gcattctgac taacgtggca gtgggtgatg
    25201 gcaatcgcca gcgcatcggc ggcatccgcc tgtggattag cgggcagttt cagcaaggtg
    25261 cggaccatat gctgcacctg gcttttttcg gcactaccca tacctaccac cgtttgcttt
    25321 acctgacgtg ccgcgtattc aaataccggc aattcctgat tcaccgccgc aacaatcgcc
    25381 acgccgcgcg cctggcccag tttcagggct gagtcagcgt tcttcgccat aaagacttgt
    25441 tcaatggcga aataatcagg ctggaactgg gtgatgattt ccgtcacgcc cgcatagatg
    25501 agcttcagac gagacggtaa atcatccact ttggtgcgta tgcatccgct acccaggtag
    25561 gacagttgcc tacctacctg gcggatgacg ccgtagccgg tcacgcgcga acccgggtca
    25621 atgccgagaa taatagccat cacgcgtctc cgttttgctg tttagcaggc atcatcagag
    25681 agtcgctgca acctcatcag agatttcacc gttatggtaa acttcctgca cgtcgtcgca
    25741 atcttccagc atatcgatca gacgcatcag tttcggcgcg gtttctgcat ccatatcagc
    25801 tttggtggac gggatcatgg aaacttccgc gctgtctgct ttcagacctg ccgcttccag
    25861 agcgtcgcgc actttaccca tttcttccca tgcagtgtag acatcaatcg cgccgtcatc
    25921 atatgtcaca acgtcttcag cacctgcttc cagagctgct tccatgatgg tgtcttcgtc
    25981 gcctttctcg aaggagatca cgcctttttt gctgaacaga taagctacgg aaccgtcagt
    26041 gccgaggtta ccgccacatt tgctgaatgc gtgacgcact tcagcaacgg tacggttgcg
    26101 gttgtcagac agacattcaa tcatgattgc cgtgccgcca ggaccgtaac cttcgtagat
    26161 gatggtttcc atgtttgcat catcatcacc gcccacacca cgtgcaattg cgcggttcag
    26221 tgtgtcacgg gtcatgttgt tagacagtgc tttatccacc gccgcacgca gacgcgggtt
    26281 agcgtccgga tcgccaccgc ccagcttagc cgcggttacc agctcacgaa tgattttagt
    26341 gaagatttta ccgcgcttag catcctgcgc agctttacga tgtctggtgt tggcccattt
    26401 actatgacct gccataaaaa tatctccaga tagccctgcc tgttcaggca gcgttaatta
    26461 caaactgttc aatcgcctgc cggttgctcc aggacttagt gagcgccgcc gcagcaggcg
    26521 catcaagcca cttgtaagcc agatgttcag tgaaaacgat ctgccgctcg tgcggaagcg
    26581 caagacagaa ccatgattcc gtattacgcg tcacgcccgg cgcatagcga tgacgtaaat
    26641 gtgaaaaaat ttcaaactct accgtgcgct gacagtcaat taaggtcagt tgttcagcga
    26701 caacatcaat ggtaacctct tcctttactt cgcgcatggc agcttgcggc gcggtttcac
    26761 cctcttccac gctgccggtt accgactgcc agaaatcggg atcgtcacgc cgctgcaaca
    26821 tcagcacccg tttcgtatct tgtgcataga tgaccactaa gatcgaaacg ggacgcttat
    26881 acactttatc cttcacgctg cctcttcgtt gactgccttc gctcatccca ttcacatagt
    26941 tatctatgct catgggagtt cactcagttg ccgccttgat gcaacgcgaa tgatttcgtg
    27001 tatttgagtc atatcagtta ttctcagcct tcttcacaac ctgaatgctc agctcagcca
    27061 gtgcagtcgg gttagcaaag ctcggtgctt cagtcatcag acacgctgcc gccgtggttt
    27121 tcgggaaggc gataacgtca cggatattgt cggtgccagt cagcagcatg gtcagacggt
    27181 caagaccgaa tgccagacct gcgtgcggcg gagtaccgta tttcagggcg tcgagcagga
    27241 agccgaattt ctcgcgctgt tcctcttcgt tgatacccag aataccaaac accgtctgct
    27301 gcatatcacc attatggata cgtactgaac caccgcccac ttcgtaacca ttgatgacca
    27361 tatcgtaagc gttcgccacc gcattttccg gtgcagcttt cagttctgca gcagtcatat
    27421 ctttcggtga ggtgaacgga tggtgcattg ccgtcaggcc gccttcaccg tcgtcttcaa
    27481 acatcgggaa gtcgataacc cacagcggtg cccatttgct ttcgtcggtc agaccaaggt
    27541 ctttacccac tttcaggagc agtgcgccca tcccgtcggc aacaattttc ttgttgtcgg
    27601 caccgaagaa aatcatatcg ccatcttgcg cggcagtacg atccaggatg gcttcgatga
    27661 tttctgcatt aaggaacttc gctaccgggc tgttgatacc ttccagacct ttcgcgcgtt
    27721 cgttaacttt gatgtaagcc agacctttcg cgccgtagat tttaacgaag ttaccgtatt
    27781 cgtcgatctg cttacgggtc agcgatgcgc cacccggaac acgtagagcg gcaacacggc
    27841 ctttcggatc gttcgctggc cccgcaaata ctgcaaactc aacagatttc agcagatcgg
    27901 caacgtcggt cagttccatc gggttacgca gatccggttt atcagaacca taacggcgtt
    27961 ctgcttccgc aaaggtcatt accgggaaat cgcccagatc cacgcccttc acttccagcc
    28021 acaaatgacg caccagcgct tccatcactt cacgcacttg cggcgcggtc atgaaagaag
    28081 tttccacatc gatctgagta aattcaggct gacgatcagc gcgcaggtct tcgtcacgga
    28141 agcatttaac gatctgatag taacggtcaa aaccggacat catcagcagc tgtttgaaca
    28201 actgtgggga ttgcggcagt gcgtagaatt tacctttgtg cacacgagaa ggcaccaggt
    28261 agtcacgcgc gccttccggc gtggctttgg tcagcatcgg agtttcgatg tcgaggaagc
    28321 cgtggtcatc cataaaacgg cgcaccaggc tggtgatttt agcgcgggtt ttcaggcgtt
    28381 gcgccatttc cgggcgacgc aggtcgaggt agcggtattt cagacgcgct tcttcggtgt
    28441 tgacgtggtt agagtcaagc ggcagaacat ctgcgcggtt gatgatagtc agcgaggacg
    28501 ccagcacttc aatttcgccg gtcgccatat cgcggttaat atttttttcg tcacgcgcac
    28561 gtacggtgcc cgtaacctga atgcagaact cattacgcag ttcagaggcc agctttaacg
    28621 cgtccgcacg atccggatcg aaaaatacct gcacgatacc ttcgcggtcg cgcatatcga
    28681 tgaagatcag gctaccaaga tcacgacgac ggttgaccca accacacaga gtcacctgct
    28741 gccccacgtg ggacaaacgg agctgtccac aatattctgt acgcatgaga tatcccttaa
    28801 cttagctgcc ggcggatgcc ccctgctgcg caggtgacca agtcgcagcg ttagctgtat
    28861 gtcacaactg aatgaaaaaa ggcggctatt atactggaaa ttctgccgca ccgtaagagc
    28921 ctggcccgcg ctggaacgcc tcgttaccac tttatatcgg gcctgaaatc agactctacg
    28981 ccagtttgct ataaaggtgt tgcccgaact cataaaaatt aacaaaattt gtcgttccgc
    29041 catcggctaa tcgcattaag gtgtgaggca cgattttgtt ttttcaggag tcagcatgct
    29101 tgaacttaat gctaaaacca ctgcgttggt ggtgatcgat ttacaagaag gcatcttacc
    29161 ttttgccggt ggtccacata ctgccgatga ggtagttaat cgcgccggga agctggcggc
    29221 gaaatttcgc gccagcggtc agcccgtgtt tctggtgcga gttggctggt ctgccgatta
    29281 cgccgaagca ttaaaacagc cggttgat
//
LOCUS       DAJTUM010000046        29035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTUM010000046 DAJTUM010000000
VERSION     DAJTUM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            99..1130
                     /gene="mdtN"
                     /locus_tag="OO471_002170"
     CDS             99..1130
                     /gene="mdtN"
                     /locus_tag="OO471_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6243429.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1130..3172
                     /gene="mdtO"
                     /locus_tag="OO471_002171"
     CDS             1130..3172
                     /gene="mdtO"
                     /locus_tag="OO471_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6243430.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3169..4635
                     /gene="mdtP"
                     /locus_tag="OO471_002172"
     CDS             3169..4635
                     /gene="mdtP"
                     /locus_tag="OO471_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6243431.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4833..5072
                     /locus_tag="OO471_002173"
     CDS             4833..5072
                     /locus_tag="OO471_002173"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243432.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5082..5252
                     /locus_tag="OO471_002174"
     CDS             5082..5252
                     /locus_tag="OO471_002174"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6243433.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5301..6713
                     /gene="fdhF"
                     /locus_tag="OO471_002175"
     CDS             5301..6713
                     /gene="fdhF"
                     /locus_tag="OO471_002175"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6243434.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6714..6980
                     /locus_tag="OO471_002176"
     CDS             6714..6980
                     /locus_tag="OO471_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6243435.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7074..7763
                     /locus_tag="OO471_002177"
     CDS             7074..7763
                     /locus_tag="OO471_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6243436.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7841..9154)
                     /gene="gltP"
                     /locus_tag="OO471_002178"
     CDS             complement(7841..9154)
                     /gene="gltP"
                     /locus_tag="OO471_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6243437.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9496..10092)
                     /gene="nrfG"
                     /locus_tag="OO471_002179"
     CDS             complement(9496..10092)
                     /gene="nrfG"
                     /locus_tag="OO471_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6243438.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10089..10472)
                     /gene="nrfF"
                     /locus_tag="OO471_002180"
     CDS             complement(10089..10472)
                     /gene="nrfF"
                     /locus_tag="OO471_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6243439.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10465..12183)
                     /locus_tag="OO471_002181"
     CDS             complement(10465..12183)
                     /locus_tag="OO471_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6243440.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12203..13159)
                     /gene="nrfD"
                     /locus_tag="OO471_002182"
     CDS             complement(12203..13159)
                     /gene="nrfD"
                     /locus_tag="OO471_002182"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6243441.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13156..13827)
                     /gene="nrfC"
                     /locus_tag="OO471_002183"
     CDS             complement(13156..13827)
                     /gene="nrfC"
                     /locus_tag="OO471_002183"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6243442.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13824..14390)
                     /gene="nrfB"
                     /locus_tag="OO471_002184"
     CDS             complement(13824..14390)
                     /gene="nrfB"
                     /locus_tag="OO471_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6243443.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14435..15871)
                     /gene="nrfA"
                     /locus_tag="OO471_002185"
     CDS             complement(14435..15871)
                     /gene="nrfA"
                     /locus_tag="OO471_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6243444.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16264..18222
                     /gene="acs"
                     /locus_tag="OO471_002186"
                     /pseudo
     CDS             16264..18222
                     /gene="acs"
                     /locus_tag="OO471_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18422..18736
                     /locus_tag="OO471_002187"
     CDS             18422..18736
                     /locus_tag="OO471_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6243445.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18733..20382
                     /gene="actP"
                     /locus_tag="OO471_002188"
     CDS             18733..20382
                     /gene="actP"
                     /locus_tag="OO471_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6243446.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20560..21852
                     /locus_tag="OO471_002189"
     CDS             20560..21852
                     /locus_tag="OO471_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6243447.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22006..23655)
                     /locus_tag="OO471_002190"
     CDS             complement(22006..23655)
                     /locus_tag="OO471_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6243448.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23806..25155)
                     /gene="ghxP"
                     /locus_tag="OO471_002191"
                     /gene_synonym="yjcD"
     CDS             complement(23806..25155)
                     /gene="ghxP"
                     /locus_tag="OO471_002191"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6243449.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25861..26325)
                     /gene="soxR"
                     /locus_tag="OO471_002192"
     CDS             complement(25861..26325)
                     /gene="soxR"
                     /locus_tag="OO471_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6243450.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26411..26734
                     /gene="soxS"
                     /locus_tag="OO471_002193"
     CDS             26411..26734
                     /gene="soxS"
                     /locus_tag="OO471_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6243451.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26737..28314)
                     /gene="pdeC"
                     /locus_tag="OO471_002194"
                     /pseudo
     CDS             complement(26737..28314)
                     /gene="pdeC"
                     /locus_tag="OO471_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28743..29024
                     /locus_tag="OO471_002195"
     CDS             28743..29024
                     /locus_tag="OO471_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6243452.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7279 a         7774 c         7351 g         6631 t
ORIGIN      
        1 tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac gccatgctgt
       61 tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg ccgaaaaaag
      121 cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt gcccttgttt
      181 tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg tcagcagata
      241 ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc accgacaacc
      301 aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac gaagccaacc
      361 tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc acccagcgta
      421 gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa aaagcccgtg
      481 ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg aaagagggtt
      541 ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca gaagcggatc
      601 ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc gtcgatgcgc
      661 tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta catctggaaa
      721 tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc tccgtcgggc
      781 aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac tggtacgtga
      841 tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc gcaacgattc
      901 gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt ggctacggcg
      961 tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct cgttctatta
     1021 actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa cctgacccgg
     1081 aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa tgagcgcgct
     1141 caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag agttaagcga
     1201 gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc tgctggtgat
     1261 tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag tgctgtttta
     1321 cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg tggttgccac
     1381 ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg aaccgttgat
     1441 ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc gcacccatcg
     1501 gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg ccatgctcga
     1561 ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc tctatccaac
     1621 cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta cgcaaatgca
     1681 tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca gcctcgcacc
     1741 gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga atgtcttttg
     1801 tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct gcgtggcaac
     1861 ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg ctgattctca
     1921 ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc agcatgccgt
     1981 tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag cggtggcggc
     2041 acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc agatgaaccc
     2101 gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg cttttaccaa
     2161 tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct gttacacctt
     2221 ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg tgatcgtcgc
     2281 taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg gggccttttg
     2341 cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg acaatattgt
     2401 cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg ccaccagctc
     2461 tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg ccacgctcga
     2521 aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc tgggtatcat
     2581 cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa gtgaagcgcg
     2641 cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa tgcggatccc
     2701 acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg gtctgcatgc
     2761 ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc aactggacag
     2821 cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg gccgcgatat
     2881 ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg cactacagcc
     2941 ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc tggcaaccgc
     3001 actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg ccatcctgcc
     3061 caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc cagactggac
     3121 agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat gatcaatcgt
     3181 caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat ttccggctgt
     3241 gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca aatcaaactg
     3301 gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg gaaacaactc
     3361 aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca caccctcgcc
     3421 gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga tgccggttca
     3481 cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc gaacggcttt
     3541 ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta ctatacggaa
     3601 gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca tcgctcagcg
     3661 gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc agtagagcta
     3721 tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta tcagatgctc
     3781 gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca ccaaagtaaa
     3841 gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca gattctggcg
     3901 gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga atctctgcgc
     3961 gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc attaccacga
     4021 gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg cccggatctg
     4081 caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc gcgggcgttg
     4141 ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca cctggatacc
     4201 ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt gccgctgttt
     4261 gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa catgatgatt
     4321 gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa cggcacgcgt
     4381 ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc aacgcgcttc
     4441 acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt acaggccact
     4501 gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga cagccgccgg
     4561 gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc ggctcccgtc
     4621 gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt cgtttcgtca
     4681 cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt gcccgtaata
     4741 tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt cgagagcgcg
     4801 tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg gtttgcccct
     4861 attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc gtccgggcgg
     4921 aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat tatggctggg
     4981 atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg atccgtcgcc
     5041 agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac gttgccgagc
     5101 gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc ggctcctcgc
     5161 gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc gttattggta
     5221 ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca ggtctgcacc
     5281 aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat accgatttag
     5341 tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat cacgtaatta
     5401 acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt gaaaccgcgc
     5461 gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg ttgaatgcga
     5521 tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct tcccgtacag
     5581 aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg gttgaagata
     5641 tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc caggcgaaaa
     5701 gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg gaaaccgtgc
     5761 gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat gcgggtgtaa
     5821 acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg ctgccggata
     5881 cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc gccaaagcct
     5941 ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg ccgcaccgcg
     6001 cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg caaactgatg
     6061 cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc gttcaggaca
     6121 tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg tcgtggggcg
     6181 agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc aaagcagttg
     6241 aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc acccgtatgg
     6301 gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt catctgtgcc
     6361 cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt cagtggcctt
     6421 gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag aagtttgata
     6481 ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc gacaaactca
     6541 ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac tcttgccgtt
     6601 cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc tacgcacaaa
     6661 tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt tgagtgcact
     6721 cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac aaaggggcga
     6781 tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc gaaagcttaa
     6841 gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag ccgatctccg
     6901 atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa actcgcctgc
     6961 gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag gctgtttttt
     7021 tatccattcg aactctttat actggttact ccctacccaa tcgtattatc aaaatgaaaa
     7081 aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac tccgagcctg
     7141 gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat tttcttgccg
     7201 acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag aaagccgccg
     7261 acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc aatccggtca
     7321 gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg ggcagtaaag
     7381 aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa ccggattatc
     7441 caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac tccgccgtcg
     7501 atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag gccgacgatg
     7561 acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt tattccgagt
     7621 actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag aacaaacaaa
     7681 aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg tgtgaagagt
     7741 ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg tcgcatcagg
     7801 catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc gcagttttat
     7861 caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca aatttgtgtt
     7921 cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc gcagtacgcg
     7981 ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc ggaataccta
     8041 cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca gcaatccctt
     8101 tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac aggtcaatgc
     8161 catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa ccatcaaggt
     8221 taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct ccgtaggctt
     8281 ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag tacgccagaa
     8341 tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac agacgtgcca
     8401 caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc agcaccagtt
     8461 tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca atcagcgcaa
     8521 acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg gtttcagaga
     8581 tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa gaaagcccca
     8641 gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc atcgacgcca
     8701 caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa ctgctttgta
     8761 ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc gacatatcca
     8821 ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg gcgacggtgg
     8881 tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt ttggcatcac
     8941 caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca atcattttga
     9001 tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc cagtcgcggc
     9061 tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata gcaaacagaa
     9121 tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca atggagcgcc
     9181 gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt gtgttgcgtt
     9241 ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg ttgcgcaacc
     9301 ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta tggcaggatt
     9361 aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat aatactgatt
     9421 ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa tgacataatc
     9481 ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat taatcgactc
     9541 gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt gccatagttc
     9601 gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca tcagagcggt
     9661 gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct gggcggtcat
     9721 atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt cagcgttctc
     9781 accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat cgttttgcca
     9841 cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag cgcggatttt
     9901 atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact gatgtagcgg
     9961 atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta acagataact
    10021 gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca ccggtatttg
    10081 aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag caataacaac
    10141 accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt atagcgaaca
    10201 aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt cccctccgac
    10261 accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga ttcgagtaag
    10321 ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc ctgttgctgt
    10381 tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa acaggtaaat
    10441 aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc atccgcttaa
    10501 caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac cgctttgtac
    10561 atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg cataccagtc
    10621 atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg cttcataaaa
    10681 acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg ccactatcgc
    10741 tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac ggaaggtgta
    10801 tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct gacgactgac
    10861 actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta ccagcgccgg
    10921 aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca gcatcaacag
    10981 accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc gtccccagcc
    11041 cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta gcactgcgca
    11101 aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa aacgcaccgc
    11161 cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc taatcagtgc
    11221 aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat gaaccgaaac
    11281 cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag taactatcgc
    11341 cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca aactgtgcag
    11401 cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat cccagaacca
    11461 ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga tccccgccgt
    11521 taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg ccgcatcaaa
    11581 ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac cgccatagcc
    11641 aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg gattgagatc
    11701 gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca gcaccacaaa
    11761 cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa atagcggatc
    11821 gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca gcagcaccca
    11881 aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt cccacgacag
    11941 caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga gaaaacaata
    12001 cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc cgatgcaagt
    12061 gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca acggggtcaa
    12121 cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa ggaatacgtc
    12181 cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag agaatgaaaa
    12241 agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg gcaggaatgc
    12301 cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc agcccggcga
    12361 caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc agcgcacgaa
    12421 ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg aagatttcac
    12481 cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa tgcgggttac
    12541 tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag atgccggaga
    12601 agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc agcgccgaca
    12661 gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc gtttccagtc
    12721 cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc ttcggcaacc
    12781 agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac agcaccagca
    12841 ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta aaactgtagt
    12901 ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg atcaacagac
    12961 cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta ctgtctgcac
    13021 cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga ccggcagaaa
    13081 taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat tcaaaatgaa
    13141 atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc ggtacagctt
    13201 cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca gcagttgcga
    13261 aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg ggcaagcttc
    13321 aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat cgcatttgtc
    13381 cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg cgatgcagta
    13441 ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg catcacgaaa
    13501 agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct ggcaagactt
    13561 acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac gaataatcgt
    13621 caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca tacaggcggt
    13681 acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat tgatattcaa
    13741 cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc cgacgccggt
    13801 gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac agacgccggg
    13861 ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt aatccgtcct
    13921 ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca gctggcgcac
    13981 gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg ttccggcaga
    14041 tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc gttaaagcgc
    14101 atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca gttggtgcag
    14161 gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg cataccttcg
    14221 gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg ggtaacttcg
    14281 tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag gctccacaac
    14341 aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat attcactcca
    14401 ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga ccgtttttac
    14461 gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc tcggccttga
    14521 tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag atatccggga
    14581 tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg gtgcgcgcat
    14641 ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt gcgtgcatat
    14701 gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg atatcgtcct
    14761 gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc gcttcgaagt
    14821 gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat tgcttacgtt
    14881 ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca caagtctggg
    14941 cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct tcggcgttct
    15001 gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga atgcccgctg
    15061 tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac agggagttag
    15121 tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact ttcatgccgt
    15181 catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc acatggcact
    15241 gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt ttaccaatgg
    15301 cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg cctttggcga
    15361 actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg atttccgggc
    15421 cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg atcagacgcg
    15481 ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct tcagcgtttt
    15541 tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca tgtccacgag
    15601 gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc cgtggatctt
    15661 ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag agatattgat
    15721 cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt tttgcgggag
    15781 ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat aagctgaaga
    15841 tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg cgccattgtt
    15901 gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt catgacattg
    15961 ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc aattgtaagt
    16021 gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac agcatgcata
    16081 actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa attaccctgc
    16141 cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa acaaaactgc
    16201 caatacccct acatttaacg cttatgccac atattattaa catcctacaa ggagaacaaa
    16261 agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg ttgcctgata
    16321 aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga taccttctgg
    16381 ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa aaatacctcc
    16441 tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa tctggcggca
    16501 aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat ctgggaaggc
    16561 gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga cgtctgccgc
    16621 ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc gatttatatg
    16681 ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg cgcgatgcat
    16741 tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat tgattccaac
    16801 tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat tccgctgaag
    16861 aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca tgtggtggta
    16921 ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg gtggcacgac
    16981 ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga agatccgctg
    17041 tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca cactaccggc
    17101 ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca tccgggtgat
    17161 atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt gctgtacggc
    17221 ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg gccgacgcct
    17281 gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac cgcacccacg
    17341 gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga ccgttcgtcg
    17401 ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga gtggtactgg
    17461 aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac cgaaaccggc
    17521 ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc ggcaacacgt
    17581 ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct ggaaggggct
    17641 accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac gctgtttggc
    17701 gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta tttcagcggc
    17761 gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt ggacgacgtg
    17821 ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct ggtggcgcat
    17881 ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg tcaggcgatc
    17941 tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta cgcagaagtc
    18001 cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct gcactggacc
    18061 gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg caaaattgcg
    18121 gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg cgtggtcgag
    18181 aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat tgccggatgc
    18241 gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag gcattgattg
    18301 ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc cgagcgttaa
    18361 ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg gagaatctgt
    18421 gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg agttagtcga
    18481 aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt atatcggctt
    18541 tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc cgaacaccag
    18601 cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg ttctcaccgg
    18661 tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag tcctgcatga
    18721 ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc tttcgcagct
    18781 aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg gcaggcgatt
    18841 attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc atcaaaacgc
    18901 gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt ccagaacggg
    18961 ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc cgcgctggtg
    19021 tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg ctggccaatc
    19081 attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc tgccgatgtg
    19141 gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg ttctctggta
    19201 gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat cgagctgctg
    19261 tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat gatgtacgtc
    19321 ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt gctgttgctg
    19381 ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag cttcaacaat
    19441 ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa accgggcgga
    19501 ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt tggtacggcg
    19561 ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga agcacgtaag
    19621 agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt tattatcggc
    19681 ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc gggccatctg
    19741 attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg caacctattc
    19801 ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc ggatctgacg
    19861 ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa aaaaggcgcg
    19921 accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg cgtgattgcg
    19981 attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg ccttgcgttt
    20041 gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg gtcgaaactg
    20101 actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt ggtgctgatg
    20161 atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat cttcccgtat
    20221 gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt cttctcggca
    20281 accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca gtttatccgc
    20341 tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc cttccccggt
    20401 cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca taatattaaa
    20461 caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga taatgcagag
    20521 aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa tggtttaaat
    20581 aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag ttcatcacat
    20641 acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt aattgacaat
    20701 aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt gattcagaaa
    20761 atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac ggacaggaaa
    20821 atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga cgatagaatt
    20881 acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt atattttata
    20941 aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa tggtggttac
    21001 gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt atccaattcg
    21061 aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat gtataaaacg
    21121 aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct cgatgactca
    21181 aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc tatgcatgtt
    21241 caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct tgactattca
    21301 aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc gccatatgtt
    21361 aatttattta aagccgacct tagtttctct aaactcgatt taattaactt tgaacatgct
    21421 gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt aattgatagc
    21481 aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg taccggctct
    21541 aatatggcta atgttaattt taataatgcc aatttaagca actgccattt caactgttct
    21601 attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt cgatgaggct
    21661 agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc cattgatagt
    21721 gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca tactggcatg
    21781 tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga tattatgcaa
    21841 cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa gagagataac
    21901 ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt gcaaaagatt
    21961 gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact gattttcctc
    22021 aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg tttcgttgct
    22081 gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca atgccgccag
    22141 acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt tacgtccatc
    22201 ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca actggttatc
    22261 gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg ccacaatcgc
    22321 cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg aatggtccgc
    22381 gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca gcgagaagag
    22441 aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt taccatccgg
    22501 caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca ccccagcgaa
    22561 agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct tcttcttcag
    22621 gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca gcatcagcgc
    22681 cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag ggtcgatttc
    22741 tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca gcaggaacac
    22801 catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca ggcgcattgc
    22861 cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg caaccgcagc
    22921 gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg gcagcaagaa
    22981 cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga atcgcagcga
    23041 gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg ctactttcat
    23101 aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca ctgccacggc
    23161 aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct gcaaaatgcc
    23221 catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca cagcatcggt
    23281 cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg gcaccaccca
    23341 gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg cgaggccgaa
    23401 aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat cagcgaacag
    23461 caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat gcaaaccaaa
    23521 cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg ggatctgaaa
    23581 gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta tcagtatggt
    23641 gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag aaagacgtta
    23701 tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc ggagaaaata
    23761 aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga tagcccagcc
    23821 acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt tcaacttgcg
    23881 ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca tgatgcctgt
    23941 tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca tcgcatcaac
    24001 aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa cgtacatcag
    24061 cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga acagaatcag
    24121 caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg ccgccgtacc
    24181 cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag agaaaacgct
    24241 gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat ctttatccag
    24301 caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata ctgccgtcat
    24361 caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa tgtccaggct
    24421 gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa cgccggagaa
    24481 atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg tcagcagaat
    24541 gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg ccagtgacat
    24601 aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa gcgggttttt
    24661 aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga taccgatccc
    24721 cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga tacccgtggc
    24781 agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga caggtacgct
    24841 aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga tggcgcaacc
    24901 aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc cggcaaccag
    24961 acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca tacctggaac
    25021 gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca cttcctgacg
    25081 gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc cggtacgcgc
    25141 tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa gatgcgtggc
    25201 ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc aatttggtgc
    25261 gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa aaaaagcaaa
    25321 cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt ttctctttta
    25381 ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg atgtgcaaga
    25441 ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt ctgagatgac
    25501 aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg gtgacataca
    25561 attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt gtgttgacgt
    25621 cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc ggaaaacaaa
    25681 ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa agagctcgat
    25741 cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag caccagtgca
    25801 aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc ttgtggcgct
    25861 ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta agcggtcgcc
    25921 cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac atccgtccag
    25981 ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc gccattggga
    26041 cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca cgccaaacgc
    26101 ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa ttgcgacata
    26161 tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac tagtaatcaa
    26221 ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc
    26281 gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc
    26341 aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa cactgaaaag
    26401 aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga ttgacgtgca
    26461 tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt caaagtggta
    26521 cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca ttcgccaacg
    26581 ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt ttgatatcgc
    26641 aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc ggcagtttga
    26701 tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg ttaactcccg
    26761 cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct tcgcaaaaaa
    26821 ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct cctcagtttc
    26881 gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga tgtgttccgc
    26941 aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat cgattttcaa
    27001 aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac caaaatcatc
    27061 aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc attttttcga
    27121 catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt
    27181 gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca
    27241 ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga tcgcggaaga
    27301 cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa
    27361 taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac aacgcttcag
    27421 cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat
    27481 gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta
    27541 gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat
    27601 gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc
    27661 gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt aaatccttca
    27721 aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt
    27781 tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc
    27841 agaagagagg gttgataacc gccacataat ttccccgctg catataggtc attttatagc
    27901 cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat
    27961 cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt
    28021 tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
    28081 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
    28141 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
    28201 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
    28261 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
    28321 caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta ccgacgtctt
    28381 tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat acctttttaa
    28441 tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac aggaatacag
    28501 aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg tacagcaata
    28561 aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca caaaattaat
    28621 gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc
    28681 acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt
    28741 tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa
    28801 tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg
    28861 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
    28921 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
    28981 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagc
//
LOCUS       DAJTUM010000047        28915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTUM010000047 DAJTUM010000000
VERSION     DAJTUM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO471_002196"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO471_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6243453.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO471_002197"
     CDS             complement(2138..2584)
                     /locus_tag="OO471_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6243454.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO471_002198"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO471_002198"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6243455.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO471_002199"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO471_002199"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6243456.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO471_002200"
     CDS             complement(5347..6009)
                     /locus_tag="OO471_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6243457.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO471_002201"
     CDS             6182..6595
                     /locus_tag="OO471_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6243458.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO471_002202"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO471_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6243459.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO471_002203"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO471_002203"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6243460.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO471_002204"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO471_002204"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6243461.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO471_002205"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO471_002205"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6243462.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO471_002206"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO471_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6243463.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO471_002207"
     tRNA            complement(9861..9948)
                     /locus_tag="OO471_002207"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO471_002208"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO471_002208"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6243464.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO471_002209"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO471_002209"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6243465.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO471_002210"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO471_002210"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6243466.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO471_002211"
     CDS             14005..14592
                     /locus_tag="OO471_002211"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6243467.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO471_002212"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO471_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6243468.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO471_002213"
     CDS             14984..15841
                     /locus_tag="OO471_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6243469.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO471_002214"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO471_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6243470.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO471_002215"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO471_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6243471.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO471_002216"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO471_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6243472.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO471_002217"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO471_002217"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6243473.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO471_002218"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO471_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO471_002219"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO471_002219"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6243474.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO471_002220"
     CDS             complement(22884..24023)
                     /locus_tag="OO471_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6243475.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO471_002221"
     CDS             complement(24069..26165)
                     /locus_tag="OO471_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6243476.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO471_002222"
     CDS             complement(26165..26911)
                     /locus_tag="OO471_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6243477.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO471_002223"
     CDS             complement(26908..27552)
                     /locus_tag="OO471_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6243478.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO471_002224"
     CDS             complement(27659..27964)
                     /locus_tag="OO471_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243479.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO471_002225"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO471_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6243480.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7321 c         7600 g         6990 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agttttaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DAJTUM010000048        28674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTUM010000048 DAJTUM010000000
VERSION     DAJTUM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..648
                     /locus_tag="OO471_002226"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO471_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="OO471_002227"
     CDS             645..1031
                     /locus_tag="OO471_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243481.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="OO471_002228"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="OO471_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6243482.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="OO471_002229"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="OO471_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="OO471_002230"
     CDS             3759..4166
                     /locus_tag="OO471_002230"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6243483.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="OO471_002231"
     CDS             4163..6451
                     /locus_tag="OO471_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6243484.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6448..7437
                     /locus_tag="OO471_002232"
     CDS             6448..7437
                     /locus_tag="OO471_002232"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243485.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="OO471_002233"
     CDS             7537..7740
                     /locus_tag="OO471_002233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243486.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="OO471_002234"
     CDS             7758..8078
                     /locus_tag="OO471_002234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243487.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="OO471_002235"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="OO471_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO471_002236"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO471_002236"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6243488.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="OO471_002237"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="OO471_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6243489.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO471_002238"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO471_002238"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6243490.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="OO471_002239"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="OO471_002239"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6243491.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="OO471_002240"
     CDS             13394..14161
                     /locus_tag="OO471_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6243492.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="OO471_002241"
     CDS             complement(14132..14872)
                     /locus_tag="OO471_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6243493.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15256..15777
                     /locus_tag="OO471_002242"
                     /pseudo
     CDS             15256..15777
                     /locus_tag="OO471_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="OO471_002243"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="OO471_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6243494.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18379)
                     /locus_tag="OO471_002244"
                     /pseudo
     CDS             complement(16667..18379)
                     /locus_tag="OO471_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="OO471_002245"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="OO471_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6243495.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="OO471_002246"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="OO471_002246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6243496.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="OO471_002247"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="OO471_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6243497.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="OO471_002248"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="OO471_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6243498.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="OO471_002249"
     CDS             21018..21470
                     /locus_tag="OO471_002249"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6243499.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="OO471_002250"
     CDS             21716..22759
                     /locus_tag="OO471_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6243500.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22821..23435
                     /locus_tag="OO471_002251"
                     /pseudo
     CDS             22821..23435
                     /locus_tag="OO471_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO471_002252"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO471_002252"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6243501.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..25669
                     /gene="gpt"
                     /locus_tag="OO471_002253"
     CDS             25211..25669
                     /gene="gpt"
                     /locus_tag="OO471_002253"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6243502.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25761..27005
                     /gene="frsA"
                     /locus_tag="OO471_002254"
     CDS             25761..27005
                     /gene="frsA"
                     /locus_tag="OO471_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6243503.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27063..27464
                     /gene="crl"
                     /locus_tag="OO471_002255"
     CDS             27063..27464
                     /gene="crl"
                     /locus_tag="OO471_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6243504.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="OO471_002256"
     CDS             complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="OO471_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6243505.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7083 a         7125 c         7607 g         6859 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaccatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgccag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ttcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttcttgctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggcgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    25561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
    25621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    25681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
    25741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    25801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    25861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    25921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
    25981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
    26041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
    26101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
    26161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
    26221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
    26281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
    26341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
    26401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
    26461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
    26521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
    26581 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
    26641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
    26701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
    26761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
    26821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
    26881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
    26941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
    27001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
    27061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
    27121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
    27181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
    27241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
    27301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
    27361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
    27421 cggcgaatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
    27481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
    27541 cattattaat attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
    27601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
    27661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
    27721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
    27781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
    27841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
    27901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
    27961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
    28021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
    28081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
    28141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
    28201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
    28261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
    28321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
    28381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
    28441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
    28501 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
    28561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
    28621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc tgaa
//
LOCUS       DAJTUM010000049        28420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTUM010000049 DAJTUM010000000
VERSION     DAJTUM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            563..1333
                     /locus_tag="OO471_002257"
     CDS             563..1333
                     /locus_tag="OO471_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6243506.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="OO471_002258"
     CDS             1324..1797
                     /locus_tag="OO471_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6243507.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="OO471_002259"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="OO471_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6243508.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="OO471_002260"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="OO471_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6243509.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="OO471_002261"
     CDS             3232..3726
                     /locus_tag="OO471_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6243510.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="OO471_002262"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="OO471_002262"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6243511.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO471_002263"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO471_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="OO471_002264"
     CDS             7556..8470
                     /locus_tag="OO471_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6243512.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="OO471_002265"
     CDS             8685..10046
                     /locus_tag="OO471_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243513.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO471_002266"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO471_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6243514.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO471_002267"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO471_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6243515.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="OO471_002268"
     CDS             12596..13885
                     /locus_tag="OO471_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6243516.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="OO471_002269"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="OO471_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6243517.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="OO471_002270"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="OO471_002270"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6243518.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="OO471_002271"
     CDS             16211..16591
                     /locus_tag="OO471_002271"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6243519.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="OO471_002272"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="OO471_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6243520.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="OO471_002273"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="OO471_002273"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6243521.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="OO471_002274"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="OO471_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6243522.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="OO471_002275"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="OO471_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6243523.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="OO471_002276"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="OO471_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="OO471_002277"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="OO471_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="OO471_002278"
     CDS             23320..23832
                     /locus_tag="OO471_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6243524.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="OO471_002279"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="OO471_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6243525.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="OO471_002280"
     CDS             26425..26628
                     /locus_tag="OO471_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6243526.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="OO471_002281"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="OO471_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6243527.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28420)
                     /locus_tag="OO471_002282"
     CDS             complement(27664..>28420)
                     /locus_tag="OO471_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR6243528.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6955 a         7425 c         7127 g         6913 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attggatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccaa gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcctgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtataa
//
LOCUS       DAJTUM010000050        28405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTUM010000050 DAJTUM010000000
VERSION     DAJTUM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO471_002283"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO471_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6243529.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OO471_002284"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OO471_002284"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6243530.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OO471_002285"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OO471_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6243531.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OO471_002286"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OO471_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6243532.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OO471_002287"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OO471_002287"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6243533.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="OO471_002288"
     CDS             6427..7056
                     /locus_tag="OO471_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6243534.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO471_002289"
     CDS             complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO471_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6243535.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8719..10074)
                     /gene="melA"
                     /locus_tag="OO471_002290"
     CDS             complement(8719..10074)
                     /gene="melA"
                     /locus_tag="OO471_002290"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6243536.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10357..11265
                     /gene="melR"
                     /locus_tag="OO471_002291"
     CDS             10357..11265
                     /gene="melR"
                     /locus_tag="OO471_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6243537.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11464..13731
                     /gene="adiA"
                     /locus_tag="OO471_002292"
     CDS             11464..13731
                     /gene="adiA"
                     /locus_tag="OO471_002292"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6243538.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14056..14817
                     /gene="adiY"
                     /locus_tag="OO471_002293"
     CDS             14056..14817
                     /gene="adiY"
                     /locus_tag="OO471_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6243539.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14954..16291
                     /gene="adiC"
                     /locus_tag="OO471_002294"
     CDS             14954..16291
                     /gene="adiC"
                     /locus_tag="OO471_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6243540.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16395..18038
                     /gene="eptA"
                     /locus_tag="OO471_002295"
     CDS             16395..18038
                     /gene="eptA"
                     /locus_tag="OO471_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6243541.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18035..18703
                     /gene="basR"
                     /locus_tag="OO471_002296"
     CDS             18035..18703
                     /gene="basR"
                     /locus_tag="OO471_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6243542.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18704..19804
                     /gene="pmrB"
                     /locus_tag="OO471_002297"
     CDS             18704..19804
                     /gene="pmrB"
                     /locus_tag="OO471_002297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6243543.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO471_002298"
     CDS             complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO471_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6243544.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO471_002299"
     CDS             complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO471_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6243545.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21747..22625)
                     /locus_tag="OO471_002300"
     CDS             complement(21747..22625)
                     /locus_tag="OO471_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6243546.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO471_002301"
     CDS             complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO471_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6243547.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="OO471_002302"
     CDS             25253..25588
                     /locus_tag="OO471_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6243548.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="OO471_002303"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="OO471_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6243549.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="OO471_002304"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="OO471_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6243550.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="OO471_002305"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="OO471_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6243551.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28405
                     /locus_tag="OO471_002306"
     CDS             28257..>28405
                     /locus_tag="OO471_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR6243552.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7297 a         7419 c         6934 g         6755 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagcc aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaacaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ctggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa
     7861 tccggtaata atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag
     7921 ctgatcgttt ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc
     7981 agacgattga ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga
     8041 aacaataaaa aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc
     8101 accgccgaca taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact
     8161 ggcaaaaaaa cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt
     8221 tggaaccagc gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta
     8281 ggtcacgcaa acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag
     8341 aaataagatt acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca
     8401 tcgcgatcgc gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat
     8461 cctcgccacc agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta
     8521 gtaatacatg aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa
     8581 tccataactg agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa
     8641 cgcccgcctt ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca
     8701 gtagcgttta gtcgcgtttt aacggtgcaa ccagcttggc agccagtcac cgtgggcggc
     8761 aatcaggtcg tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg
     8821 gtccatcatc gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag
     8881 cagcgtctgt acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc
     8941 gactttggtc ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc
     9001 ttgtggcagg ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc
     9061 gccagtccag atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat
     9121 ccgggaggcg tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca
     9181 gcgtttcggg tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag
     9241 cttaataaac cacggcgtat attcggcaaa atgttctgat gattccgtga cgaagtagcc
     9301 cagctttttg aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt
     9361 tggcttcggt gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta
     9421 actgccgtcg gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc
     9481 gcaacggtaa cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt
     9541 tccctgcacc gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc
     9601 ccaggtattc atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc
     9661 cgtcatgttc tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc
     9721 gcccggcccc aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa
     9781 atcagtcacc gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc
     9841 atcctctaag gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga
     9901 atccatcagc ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat
     9961 cagggcaata tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt
    10021 aacgaaaatc gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat
    10081 ctcctggctt gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt
    10141 ccaggttgag cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta
    10201 tgggaataaa ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc
    10261 gcccctctgg gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct
    10321 gccataattc tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc
    10381 agcagcgacg aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg
    10441 gagattgagt ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa
    10501 gtgaatgtgc ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc
    10561 aatcagggtc atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga
    10621 acctgtcaga gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg
    10681 gataaagacc tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag
    10741 caacttagcc cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa
    10801 attcgccagc tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc
    10861 tgggaaccga ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat
    10921 gcgcaatttt atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg
    10981 accatcaacg atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc
    11041 cagcgggtca tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt
    11101 cgcgcgttac tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt
    11161 cgttcaagta gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa
    11221 taccgcaaac ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat
    11281 cagccaaaaa aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac
    11341 ataacgtatt gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca
    11401 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
    11461 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
    11521 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
    11581 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
    11641 agctatcaaa tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
    11701 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
    11761 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
    11821 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
    11881 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
    11941 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
    12001 tactatggtg aaaatctgtt ccgtaccgac atgggcatcg aacgaacttc cctcggttct
    12061 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
    12121 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
    12181 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
    12241 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
    12301 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc
    12361 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc
    12421 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
    12481 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
    12541 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
    12601 gccacccact ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
    12661 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
    12721 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
    12781 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
    12841 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
    12901 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
    12961 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
    13021 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
    13081 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
    13141 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
    13201 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
    13261 tccttcaaac accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
    13321 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
    13381 gcctggctga aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg
    13441 gtggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
    13501 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
    13561 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
    13621 tatttacgct cgctgcaatc ctgggaccac catttcccta gatttgaaca cgaaactgaa
    13681 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
    13741 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
    13801 ggcaacgttt tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac
    13861 cccacgcttt tttttgcctg ttatttatcc tgtaaaaaaa tatgtacatg agaaattact
    13921 ataaaaattt gtaatattag taaaactcgt tatttttatg catgtttata ttcatcatgc
    13981 aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct tttattcgca
    14041 ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt attgactgaa
    14101 aaagatgtct ggataagggt gaatgggaaa gaacctatta gccttaaagc taaccatatg
    14161 gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa cactttggtt
    14221 gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa agatctctcg
    14281 caaataccag tatggcaacg tagcgctacg cccatactca ccctgccatg cctgacgcca
    14341 gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga gtcagaaaag
    14401 gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag taaaaaattc
    14461 ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagcgttta tcaaattatt
    14521 gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt atgtcttagc
    14581 ccaagtctgt taaagaaaaa gttgaaaagc gaaaacacca gttatagcca aataatcacc
    14641 acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag
    14701 gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt taaagacttc
    14761 tacggtatga cgccgctgca ttatgttagt cagcacagag aacgcactgt cgcctgattt
    14821 ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt cgataataat
    14881 tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag ctcgccttcg
    14941 caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa tccccgtcac
    15001 cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg caaacctggc
    15061 ctccactggc gggattgcca tttatggatg gttggtgacg attatcggtg cgctggggct
    15121 ctcgatggta tacgccaaaa tgtcgttcct cgacccaagt cctggtggtt cttacgctta
    15181 cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct actggctggc
    15241 ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa gttacttctt
    15301 cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc tgtggatctt
    15361 cgtcctgctg aacattgtcg gtccgaaaat gatcacccgc gtgcaggccg ttgccactgt
    15421 gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt tccgtggtga
    15481 aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcga ttcaaagtac
    15541 ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt
    15601 ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt ggtggggtat tgattgccgc
    15661 cgtttgctat gtgctctcca ccaccgcgat tatggggatg atccctaatg ccgcactgcg
    15721 cgtttctgcc tcgccattcg gtgatgccgc acggatggcg ttgggtgaca ccgctggggc
    15781 cattgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct ggacgttgct
    15841 ggcgggtcaa acggcgaaag ccgctgccga tgacggactg ttcccaccga tttttgcccg
    15901 tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt tgatgaccat
    15961 cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg tttcttccgt
    16021 ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac tgctgctcgg
    16081 ccacggtcac tttggtaaag cacgcccggc atatctggct gttactacca ttgccttcct
    16141 ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt catttgtcac
    16201 cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctacata aaaacccgta
    16261 tcccttagat gcaccaataa gcaaagatta attcccctta atccagcaaa cataaaagcc
    16321 aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc actttgttcg
    16381 atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat ttactcgcct
    16441 ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt tttaaacagg
    16501 tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg tcgatgccgg
    16561 ttgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta tggcttaatc
    16621 gaccactggc ctgcctgttt attctgattg gcgcggctgc acaatatttc ataatgactt
    16681 acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact ccagcagaaa
    16741 gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc gtgcttgctg
    16801 agctgattgc ctgctggcta aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt
    16861 tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc gcactgtttt
    16921 ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc ttaagcccct
    16981 ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca aatctgccgc
    17041 tggtgcgaat tggtgaagac gcgcaccgca acccgttaat gcagaacgaa aaacgtaaaa
    17101 atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct
    17161 acccgcgtga aactaacccg cggctggcga aagataacgt ggtctatttc cctaataccg
    17221 catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat atgccgcgtg
    17281 agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc attcagcgag
    17341 cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg
    17401 tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac ggcgaatgct
    17461 atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag ggtgatggcg
    17521 tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc tatccgccgc
    17581 agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt accaaagagc
    17641 aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt gataaagcga
    17701 ttaatctgct taaagaacat caggataaat ttaccaccag cctggtttat ctttctgacc
    17761 acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat gccatcgccc
    17821 cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc ggaggattat caaaagcggt
    17881 atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca
    17941 atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac caggccgcgg
    18001 atgatattct gcaaacttgc aggagagtga gtaaatgaaa attctgattg ttgaagacga
    18061 tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg cgtgcgatgg
    18121 cgtgacaacc gcgcggatgg cggaacaaag ccttgaggac ggtcattaca gcctggtggt
    18181 actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta tccggcagaa
    18241 aaaatacacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc
    18301 cgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg aagagttaca
    18361 cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg agctgattgt
    18421 tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg aagagttgat
    18481 tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag gcagtccggt
    18541 acatcgggaa attctctaca acgacatcta taactgggac aatgaaccct cgaccaacac
    18601 cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta tccgcaccgt
    18661 gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc tgatgcgttt
    18721 tctgcgccga cctatatcgc tgcgccaacg gctgatattg accatcgggg ccattttgtt
    18781 ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc agattcagct
    18841 gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc gtgagatccg
    18901 cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga cgctatttat
    18961 ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa aagagctgga
    19021 agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc tcgaaatcga
    19081 agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc tggataacga
    19141 aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt
    19201 gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag tggcacggct
    19261 tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg gacagtcatt
    19321 ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc cctcgtatga
    19381 cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag agagcgccgc
    19441 cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa acctggtaga
    19501 aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc aagaagatgg
    19561 cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta aatgcgggga
    19621 gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc tggggttaag
    19681 tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa accggcaaga
    19741 gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg caaaccagat
    19801 ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt cataaacacg
    19861 gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca gagattaagt
    19921 gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat ccgtttcagc
    19981 ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct gcaaatcggc
    20041 aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct cgacgagaat
    20101 ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg gacgatttgc
    20161 cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca tcaggtaata
    20221 ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg ttggcgtcag
    20281 accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt
    20341 cgggaacatc gctggcaagg tagaagccat aacgcccata aagcagttaa ggatcaccgc
    20401 cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca gaataaacgc
    20461 cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac gacggccaaa
    20521 acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca taatggcgat
    20581 aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat aactcggcat
    20641 ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa
    20701 caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc catcctgcaa
    20761 accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg gagtttcttc
    20821 cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa agaacggaat
    20881 acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta acaccaccac
    20941 gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc ccataaagcc
    21001 acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat attcaccacc
    21061 gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc aaataccaat
    21121 cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa tcacaatagt
    21181 gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga agagtccgcc
    21241 aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca tctgcacgct
    21301 ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac cataaacacc
    21361 aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt tacgcagttt
    21421 accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt tccttttcag
    21481 catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg
    21541 ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta gcgggtttgc
    21601 ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat tgggcagtta
    21661 atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat ggatttcgtt
    21721 taggactcat tgatgtaact gattatttac gccaaattta cagagaagat ttcctcgacc
    21781 attgccccca ccaaacgctg cgctgaacag agtcgtggca tccccagcgc gacagtttgc
    21841 cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca ctttcccagc
    21901 caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc aaaccactga
    21961 tggtaatagt gacgcgtagt cggtgccgca ttgatactct ctgccagatg ttgtgtcgct
    22021 ttctcgcgca aatcacccag caacaacacc accgcttgcg ggcttcccag acggcaggca
    22081 tcgccaaatg caccccagcg caactcctcc agaacgataa aacagcgccc aggcaacgac
    22141 caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg atcacctgcc
    22201 tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg ggctgaaaac
    22261 cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg ctggcggtga
    22321 tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagcgccag atgaccggga
    22381 tccagcatcg acgccagttt ttccaccatt gtcagtcgca ggctaagcgg cgcatttaac
    22441 atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa gaagttcctg catcagtcgt
    22501 tcacgaaact gctgctgatg ggcttgagga tggcgggcat catcgccctg cgccagatcc
    22561 actaaaaagc gcggatgaac cgactccagc acgcgaccgg ggccgtccag taacgtcttg
    22621 gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc tgagcgtcgg
    22681 tgtaaatcca gctgtgagtc atcacagtct gctgcaattg ctgctgcatc agcctgacca
    22741 ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt aattctgtca
    22801 aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat tcatcgacct
    22861 gctctgccag aactttagcc atttgttggc aatagagatc gacttctgcg cttaatgctc
    22921 gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc cgtccccagt
    22981 cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc gtgctctgct
    23041 cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg ttgaagtggg
    23101 gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac tcgctcaatt
    23161 ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg caaagggtgg
    23221 attccatctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc gccgtctgta
    23281 acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc tgaaacaagg
    23341 cggctaactg cgcattcacc gcatcttgct gttgacgcag aaagtggttg gcggaggtca
    23401 gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg agttgcaata
    23461 gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa gcgacgttta
    23521 acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc aacagtttat
    23581 gcgcagcaga tcgcagagca tacaatgagg cgttagcgta agcggcatga agcaacgcct
    23641 gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga cgaatatgtt
    23701 cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg gcaaaatctt
    23761 ccacccagcg ttgttgctct ggcgctggta acttaccgtt gttggctaac tcatagcgcg
    23821 cccgattcgc caggtagccc cacatcgacg acaccgggaa tatctgctgt ggcgtaatac
    23881 agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg tcactgttac
    23941 gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc cccaccgcca
    24001 aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa tccagcaccg
    24061 ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga tgcggttgcc
    24121 cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa ctttccagcc
    24181 ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg gcgtaagcag
    24241 aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc aaacttttca
    24301 gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg ccattttcga
    24361 tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc agatgcttaa
    24421 tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa acaatcgatt ggcgcgacat
    24481 gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg gcgaataagc gtcggcagcg
    24541 cagtcattgg gcgattacga ttaggcagaa cctccgtacc aacaatggca ttaatggtgg
    24601 ttgatttccc tgctttcatg gtaccgacaa ttgccagcac catttccagt cgggaaattt
    24661 tacacaactc attattcagc atcgcgtgac gttcggcgat attaggctga ctccagggta
    24721 aagccagttg tggcgcgtcg tctccgggta cagagagagg cattttttcc agtaactgca
    24781 actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca tacagggtct
    24841 gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt agccagattg
    24901 tttttgagtt ctgttttcgg cttttataat tactgcaaga aataatttta tatttagtgt
    24961 gttgtttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca acctt
//
LOCUS       DAJTUM010000051        28354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTUM010000051 DAJTUM010000000
VERSION     DAJTUM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO471_002307"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO471_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO471_002308"
     CDS             complement(1023..2045)
                     /locus_tag="OO471_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6243553.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO471_002309"
     CDS             complement(2059..3561)
                     /locus_tag="OO471_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6243554.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO471_002310"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO471_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO471_002311"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO471_002311"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6243555.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO471_002312"
     CDS             complement(5877..6218)
                     /locus_tag="OO471_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6243556.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO471_002313"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO471_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6243557.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO471_002314"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO471_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6243558.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO471_002315"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO471_002315"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6243559.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO471_002316"
     CDS             12897..14240
                     /locus_tag="OO471_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6243560.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO471_002317"
     CDS             complement(14302..14508)
                     /locus_tag="OO471_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6243561.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO471_002318"
     CDS             14833..15387
                     /locus_tag="OO471_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6243562.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO471_002319"
     CDS             complement(15450..16388)
                     /locus_tag="OO471_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243563.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO471_002320"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO471_002320"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6243564.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO471_002321"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO471_002321"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6243565.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO471_002322"
     CDS             complement(19599..19979)
                     /locus_tag="OO471_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6243566.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO471_002323"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO471_002323"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6243567.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO471_002324"
     CDS             21037..22002
                     /locus_tag="OO471_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6243568.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO471_002325"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO471_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6243569.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO471_002326"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO471_002326"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6243570.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO471_002327"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO471_002327"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6243571.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO471_002328"
     CDS             25376..25921
                     /locus_tag="OO471_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6243572.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO471_002329"
     CDS             complement(25905..26552)
                     /locus_tag="OO471_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6243573.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO471_002330"
     CDS             26764..27555
                     /locus_tag="OO471_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6243574.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28354
                     /locus_tag="OO471_002331"
     CDS             27565..>28354
                     /locus_tag="OO471_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6243575.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQK"
BASE COUNT         6955 a         7549 c         6991 g         6859 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tataaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaag
//
LOCUS       DAJTUM010000052        27601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTUM010000052 DAJTUM010000000
VERSION     DAJTUM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO471_002332"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO471_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6243576.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO471_002333"
     CDS             complement(1614..1937)
                     /locus_tag="OO471_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6243577.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO471_002334"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO471_002334"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6243578.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO471_002335"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO471_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6243579.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO471_002336"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO471_002336"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6243580.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO471_002337"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO471_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6243581.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO471_002338"
     CDS             7537..8574
                     /locus_tag="OO471_002338"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6243582.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO471_002339"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO471_002339"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6243583.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO471_002340"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO471_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6243584.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO471_002341"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO471_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6243585.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO471_002342"
     CDS             10745..11626
                     /locus_tag="OO471_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6243586.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO471_002343"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO471_002343"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6243587.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO471_002344"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO471_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6243588.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO471_002345"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO471_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO471_002346"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO471_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6243589.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO471_002347"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO471_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6243590.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO471_002348"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO471_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6243591.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO471_002349"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO471_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6243592.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO471_002350"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO471_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6243593.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO471_002351"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO471_002351"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6243594.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO471_002352"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO471_002352"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6243595.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO471_002353"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO471_002353"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6243596.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO471_002354"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO471_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6243597.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO471_002355"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO471_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6243598.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO471_002356"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO471_002356"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6243599.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO471_002357"
     CDS             24893..25273
                     /locus_tag="OO471_002357"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6243600.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO471_002358"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO471_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6243601.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO471_002359"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO471_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6243602.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="OO471_002360"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="OO471_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6864 c         7236 g         6693 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAJTUM010000053        26706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTUM010000053 DAJTUM010000000
VERSION     DAJTUM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..112
                     /locus_tag="OO471_002361"
     CDS             <1..112
                     /locus_tag="OO471_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6243603.1"
                     /translation="MRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(109..249)
                     /locus_tag="OO471_002362"
     CDS             complement(109..249)
                     /locus_tag="OO471_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6243604.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            491..784
                     /locus_tag="OO471_002363"
     CDS             491..784
                     /locus_tag="OO471_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6243605.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            856..1515
                     /locus_tag="OO471_002364"
     CDS             856..1515
                     /locus_tag="OO471_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6243606.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1607..2053
                     /locus_tag="OO471_002365"
     CDS             1607..2053
                     /locus_tag="OO471_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6243607.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2260..3160)
                     /gene="hlyE"
                     /locus_tag="OO471_002366"
                     /pseudo
     CDS             complement(2260..3160)
                     /gene="hlyE"
                     /locus_tag="OO471_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3533..3952
                     /locus_tag="OO471_002367"
     CDS             3533..3952
                     /locus_tag="OO471_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6243608.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3952..5220
                     /gene="umuC"
                     /locus_tag="OO471_002368"
     CDS             3952..5220
                     /gene="umuC"
                     /locus_tag="OO471_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6243609.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5266..5796)
                     /gene="dsbB"
                     /locus_tag="OO471_002369"
     CDS             complement(5266..5796)
                     /gene="dsbB"
                     /locus_tag="OO471_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6243610.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5942..7483)
                     /gene="nhaB"
                     /locus_tag="OO471_002370"
     CDS             complement(5942..7483)
                     /gene="nhaB"
                     /locus_tag="OO471_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6243611.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7704..8423
                     /gene="fadR"
                     /locus_tag="OO471_002371"
     CDS             7704..8423
                     /gene="fadR"
                     /locus_tag="OO471_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6243612.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8475..10007)
                     /locus_tag="OO471_002372"
     CDS             complement(8475..10007)
                     /locus_tag="OO471_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6243613.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10337..11635
                     /gene="dadA"
                     /locus_tag="OO471_002373"
     CDS             10337..11635
                     /gene="dadA"
                     /locus_tag="OO471_002373"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6243614.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11645..12715
                     /gene="dadX"
                     /locus_tag="OO471_002374"
     CDS             11645..12715
                     /gene="dadX"
                     /locus_tag="OO471_002374"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6243615.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12883..14297)
                     /locus_tag="OO471_002375"
                     /pseudo
     CDS             complement(12883..14297)
                     /locus_tag="OO471_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14908..16644)
                     /locus_tag="OO471_002376"
     CDS             complement(14908..16644)
                     /locus_tag="OO471_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6243616.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16739..17653)
                     /gene="ldcA"
                     /locus_tag="OO471_002377"
     CDS             complement(16739..17653)
                     /gene="ldcA"
                     /locus_tag="OO471_002377"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6243617.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17753..18364
                     /gene="emtA"
                     /locus_tag="OO471_002378"
     CDS             17753..18364
                     /gene="emtA"
                     /locus_tag="OO471_002378"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6243618.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18366..19100)
                     /locus_tag="OO471_002379"
     CDS             complement(18366..19100)
                     /locus_tag="OO471_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6243619.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19301..19555
                     /locus_tag="OO471_002380"
     CDS             19301..19555
                     /locus_tag="OO471_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6243620.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19605..21065)
                     /locus_tag="OO471_002381"
     CDS             complement(19605..21065)
                     /locus_tag="OO471_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6243621.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21002..21559)
                     /locus_tag="OO471_002382"
     CDS             complement(21002..21559)
                     /locus_tag="OO471_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6243622.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21585..22439)
                     /locus_tag="OO471_002383"
     CDS             complement(21585..22439)
                     /locus_tag="OO471_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6243623.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22439..23248)
                     /locus_tag="OO471_002384"
     CDS             complement(22439..23248)
                     /locus_tag="OO471_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6243624.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23258..24016)
                     /locus_tag="OO471_002385"
     CDS             complement(23258..24016)
                     /locus_tag="OO471_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6243625.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24013..24993)
                     /locus_tag="OO471_002386"
                     /pseudo
     CDS             complement(24013..24993)
                     /locus_tag="OO471_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(24993..26015)
                     /locus_tag="OO471_002387"
     CDS             complement(24993..26015)
                     /locus_tag="OO471_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6243626.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26365..>26706
                     /locus_tag="OO471_002388"
     CDS             26365..>26706
                     /locus_tag="OO471_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243627.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6709 a         6500 c         6897 g         6600 t
ORIGIN      
        1 aatgcgggca acggtgttta attatatcga atgtgattac aatcggtggc ggcggcacag
       61 ttggtgtggc ggcctcagtc cggaacaatt tgaaaaccag aacctcgctt aggcctgtgt
      121 ccatattacg tgggtaggat cactatggcc tttgctgaac tcaacattga cattcttacc
      181 cgcagcaaag ctcgtggagg ttagcagtgc aagcagcaat acagccttac tgatgctctt
      241 gattttcatt ggtagtctcc tgttcagaat gagtacgatg aactgttata atataacaat
      301 cccttacggg agaattccag tggcttttct gcgttcggta aaatcccggc gcggcatttt
      361 ttgccggggg gattacttat aaaggggcat ggactcaaca aaccgcagaa atctacagct
      421 ggctggcttg tggcgcgggt ttcattgatg ttagcttatg ccaaaacctg gcatcttaaa
      481 gagtaaaagt atgttttgtg cgatttatcg aagcagcaag cgtgaccaga cctatttata
      541 tgtcgaaaaa aaagacgatt tttcgcgtgt tcctgaggaa ctgatgaaag gttttggtca
      601 gcctcagtta gcgatgattc tgccgttgga tgggcggaag aaactggtca atgctgatat
      661 tgaaaaggtt aaactggcat taaccgagca aggttactat ttgcagttac cgccaccacc
      721 cgaagatttg ctgaagcaac atctttccgt catggggcag aaaacagacg acactaacaa
      781 ataaccgata tccggcggtg gcattatctt tgtcggcgcg ggttttcata tccacgataa
      841 ggtgagggga acgttatgta tcaacatcac aactggcaag gtgcgctgct ggattatccg
      901 gtgagtaaag tagtctgtgt tggcagtaac tatgccaaac atattaaaga gatgggcagc
      961 gcagtgcccg aagagccagt gctgtttatt aaaccagaaa cggcactgtg cgatctgcgg
     1021 cagccattgg cgatcccatc cgatttcggt tcagttcatc atgaagtcga actggcggtg
     1081 ttgattggcg cgacgctgcg tcaggcaacg gaagagcatg tccgcaaagc cattgccggt
     1141 tacggcgtgg cgcttgatct gacattgcgt gatgttcagg gaaaaatgaa gaaagccggg
     1201 cagccgtggg aaaaggctaa agcgtttgat aactcttgtc cgctttccgg gtttatcccc
     1261 gcggcggaat ttaccggcga tccacaaaat acaacgctgg gcctgagcgt aaacggcgaa
     1321 aaacgccagc aaggtacgac tgcggacatg atccataaaa tcgttccgtt gatcgcttat
     1381 atgagcaagt tttttaccct caaggccggt gacgttgtgt tgacaggcac gcctgatggc
     1441 gtcggcccgt tgcaaagcgg tgatgagctg acagtcactt tcgatgggca ttctttgaca
     1501 actcgcgttt tgtaatactt tttgccgcca gaaagcggcg gcaaaacttg catcgctgtg
     1561 ccagactggt tataaggtgc gttttaacgt aatggcggaa cacctgatga gcgatgtacc
     1621 tttctggcaa agtaaaaccc tggacgaaat gagcgatgcg gaatgggagt cgttgtgtga
     1681 tggttgcggt cagtgttgct tgcataaact gatggatgaa gacaccgacg aaatctactt
     1741 cactaacgtc gcctgtcgcc agctcaatat taaaacctgt caatgtcgga actacgaacg
     1801 tcgtttcgag tttgaacccg actgcattaa attaacccgt gaaaatctgc caacattcga
     1861 atggctgcca atgacctgcg cttatcggtt gctggcggaa ggtaaagatt tacctgcgtg
     1921 gcatccgcta cttactggtt cgaaagcggc aatgcatggt gaacgtatct ctgtgcgtca
     1981 tatcgcagtg aaagaatcag aagtgattga ctggcaggat catatcttaa ataaacctga
     2041 ctgggcgcag tgataaattt aatagatgct gatgggggct ttcttaatat acgtctggat
     2101 gctcgccatt tcgtagccct gtttttgaaa gcgctctgtc ataatgagag ttacccgata
     2161 ccttattatc aacaggtaac tctcaatctt taaaaaacgt ctgtgttttt aaatgtgata
     2221 aaccttatac cttgagtaca tggagagaat aatcgcttat cagacttcag gtacttcaaa
     2281 gagtgtcttt ttaccgtgtc ttttctgata ctcattacag gtgttaatca tttttttggc
     2341 cgctgctttt agcaaagaaa gcattaaatc atcataatca acatagaatc tggttgtttc
     2401 agtttccgtt tttatctccc cgatggcggc tatttcggtg gttaatttca atttggcggc
     2461 atcgatatct ttattcgctt gtttaaccgt gttagacagg gtggtaaaga aactctgcac
     2521 agattttaac ttgttcttca attctggaat cagtttccct tcaactacgc ccgcagcaat
     2581 agaataggaa atgattaaac caaatggacc ggcgacgaca tcggctgcgg caccggcata
     2641 cgcttccttc ctgattttat ctacctgtga ctggaaatag ctgctttttt ctgaaaaatc
     2701 attggttaac tggctatcta acgccagcag tttcccggaa gcgttgttga aactttgtga
     2761 gcttaccagc agggattttt gcgcttcatt cagcttcgtg atgccgtcat ccagtacctt
     2821 aatgaggggc ggatgctttc ttctcattgt actcatcaaa tagcaaaata tacgctgcga
     2881 gcaattgcgt cgcaacacca caccattcat acactgtttg ggttgcttca aaatacttat
     2941 cctggctatc cataagtaag gttttaatat cgccgactaa aacggaggct gcctgtgaat
     3001 actcctgttt aaagcgactt aactctttta tggtttcatc aaaggtctgc caggggatga
     3061 cctgatcgag atatttatta taaagatcta atgctccatc tgcggtttcg attgcgtttt
     3121 taactacttc taccgtttta tctgcaacga tttcagtcat aatcattcgc ctctttaaat
     3181 atataaattg taatgaaact cctgttttac aactattaat aaattttact tcatctaatt
     3241 catagttagc cgggcgggat gcgtcaatgt ctttatttct attaatatga taaatatcaa
     3301 acaatgttta atgtcattat ggcgaatgct tctattctat ttttttgccg ggtgatattt
     3361 ttcatttctg ctggatgagc gtcgtcgcca gaaggccacg tgagcacagg ataagagaac
     3421 gaaaaatcag cagtctatgc agcgacaaat attgatagcc tgaatcagta ttgatctgct
     3481 ggcaagaaca gaccactgta tataaaaaca gtataacttc aggcagatta ttatgttgtt
     3541 tatcaagcct gcggatctcc gcgaaattgt gacttttccg ctatttagcg atcttgttca
     3601 gtgtggcttt ccttcaccgg cagcagatta cgttgaacag cgcatcgatc tgaatcaact
     3661 gttgatccag catcccagcg cgacttactt cgtcaaagca agtggtgatt ctatgattga
     3721 tggtggaatt agtgacggtg atttactgat tgtcgatagt gctattaccg ccagccatgg
     3781 tgatattgtc atcgctgctg ttgacggcga gtttacggtg aaaaaattgc aactacgccc
     3841 gacggtacag cttattccca tgaacagtgc gtactcgccc attaccatca gtagcgaaga
     3901 tacgctggat gtctttggtg tggtgatcca cgtcgttaag gcgatgcgct gatgtttgcc
     3961 ctctgtgatg taaacgcgtt ttatgccagc tgtgagacgg tgtttcgccc tgatttatgg
     4021 ggtaaaccgg tggttgtgct atcgaataat gacggttgcg ttatcgcccg aaacgctgag
     4081 gcaaaggcgc tgggcgttaa aatgggcgat ccctggttca aacaaaaaga tctgtttcgt
     4141 cgctgtggtg tggtttgctt tagcagcaat tatgagcttt acgcagacat gagcaatcgg
     4201 gtgatgtcga cgctggaaga actatcgccc cgcgtcgaga tttacagtat tgatgaagca
     4261 ttctgcgatc tgacaggtgt gcgtaattgt cgcgatctga ctgattttgg cagagaaatt
     4321 cgcgcaacgg tgctacaacg tacccatctt actgttggtg tggggatcgc ccagaccaaa
     4381 acgttggcta agcttgccaa tcatgcggca aaaaaatggc agcggcagac gggtggggtg
     4441 gtggatttat caaatctgga acgccagcgt aaattaatgt ctgctctccc cgtggatgac
     4501 gtctggggga ttggacggcg gatcagcaaa aaactggacg cgatggggat caaaaccgtt
     4561 ctcgatttgg cggatacaga tatccggttt atccgtaaac attttaatgt catgctcgaa
     4621 agaacggtgc gtgaactgcg cggcgaaccc tgtttgcaac tggaagagtt tgcaccgacg
     4681 aagcaggaaa ttatctgttc ccgctcgttt ggtgaacgca tcacggatta tacgtcgatg
     4741 cggcaggcca tttgtagtta cgctgcccgg gcggcggaaa aacttcgcag tgagcatcaa
     4801 tattgtcggt ttatctccac atttattaag acgtcaccat ttgcgctcaa tgaaccttat
     4861 tacggcaata gcgcgtcggt aaaactgctg acgcccactc aggacagcag ggatatcatt
     4921 aacgctgcta cgcgatctct ggatgccatc tggcaagcgg gccatcgcta ccaaaaagcg
     4981 ggcgtgatgc tgggggattt cttcagtcag ggagtcgcgc agctcaattt attcgatgac
     5041 aacgcaccgc gccccgggag tgagcaattg atggcggtaa tggatactct gaatgctaaa
     5101 gagggcagag gaacactcta ttttgccggg caggggatcc aacaacaatg gcagatgaag
     5161 cgagctatgc tttcaccacg ttatacaacg cgaagttctg atttactgcg ggtcaaataa
     5221 atatagcggc aggaaaaaag cgctcccgca ggagcgccga atggattagc gaccgaacag
     5281 atcacgtttt ttcgctttaa acggctggga aatcaccacc agcaccgcga caatcaggta
     5341 agcgataaaa ataccgagca gccactgcgg catttccata cctaaaaaat cccactgacg
     5401 ctcggcgcaa tcgccagagg cgacaaacac ttgcggcacc catttatcca gcggcagcca
     5461 ttccgggaaa cgaaccataa aatcacaggt ggcaaatggc gaaggataga gctgaagcat
     5521 ggtgtgctcg taagttaact gcacaccgcg gaacgcacta tacaaccaga taaccatcgc
     5581 tacataacgc agcggagttt tcggggcgat cgcgccaatc agcgcagcac ccagaacgcc
     5641 gaataacgcg cagcgttcat aaatacagag cacgcaaggt ttcagtaaca tcacatgctg
     5701 gaaccacagc gccgtcagtt ccagtgccag agcagtaaac gccatcaaca gccacgcgcc
     5761 ccggccttgt gaacattggt tcaaaaatcg caacataatc atttccctgc aatatgcata
     5821 gagtgcgcag tttaaaccaa ttcattcgct gcgccaccag gaggcagcaa aaataggacg
     5881 caattggaca attatcgggc gaaaaggcaa accgggcatt tatatgcccg gtaagttgtt
     5941 atcaaagcgt tgctatccag cccatttgca taaaccattc ggttacaggg gcaagcgtaa
     6001 actcgacgca gagcaagccg acgagtgtca gtacgagggt gtaaggcagg gccatccaca
     6061 ccatgcggcc ataagagagg cgaatcaatg gcgcgagtgc agaggtcagc aggaacagga
     6121 acgcagcctg accgttcggc gtagcgacag agggcagatt ggtaccggta ttaatggcga
     6181 ccgccagcag ctcgtattgc ttcaacgtga tagcgccact ttccattgcc gctttcgctt
     6241 cgttgatata aatcgtcccc acgaagacgt tatccgaaat ggatgacagc agaccgttga
     6301 aaatatagaa cagcgacagc tgagcatgtt cagatgcctg caacacaaac tgaataatcg
     6361 gcgaaaacag ttgttggtcg ataatcaccg cgactaccga gaaaaacacc gtcaacagtg
     6421 cggtgaatgg cagagattcg gtgaaggctt taccgatagc atgttcatcg gtgacaccgg
     6481 tcaatgatgt tgccagaata atgactgaca aaccaatcaa gccaacttcc gccagatgca
     6541 acgccagcgc agtcaccagc cagacgccaa taatcgcctg gacaatcaga cggattttat
     6601 cctgacgggt acgctggtgg cggctttgat cgtcaaactg ttgcaacact tcgcggactt
     6661 tctccggcag cgtttcaccg tagccaaacc aacgcagctt ctctaccagc aggcaggtta
     6721 acaggccaca aatcagaacc ggaacggtca ccggcgacat gcgcaggaag aaatcgccaa
     6781 aatgccagcc agccgcttta gcgatgatca ggttctgtgg ttcgcccacc atggtcatta
     6841 cgccgcctaa tgcggtgccg acaccggcat gcatcatcag gctacgcaga aagccacgga
     6901 actgttccag aaccacgttg taatgcttgt cgatatgact atcgtcttgc aggtcggtgt
     6961 cttcggtacg ggaagaggct acgcgatgat aaataccata aaaaccgact gcaacgctga
     7021 tcaccaccgc cacgacggtt aaggcatcga ggaacgcgga gaggaacgct gccgccacgc
     7081 aaaaagagag cgacagcagc attttggagc gaatgcttaa cagcaaacgg gtaaatatga
     7141 acagcaacag ctgtttcata aaatagatac ccgccaccat aaacatcaat aacagcaaga
     7201 cttcaagatt tgccgccacc tcttcacgga cgtgttccgc gctggtcatg ccgatgaata
     7261 ccgcttcgat agccaacaga ccaccgggga gtagcgggta gcatttcagg gccatcgcca
     7321 gagtgaaaat aaattccgct accagcaacc agcccgcgac gaaagggctg atgaggaaaa
     7381 ttaacgggtt tacgattaag aaaatgatga gggcgagttt gtaccagtcg ggggactggc
     7441 ccaaaaaatt gcgccatagc gcgcggcccc aggagatctc catgatggtt tcccttacct
     7501 tacaaataat caatgatgtt tttatgttta aacgcaaagc ttaacggtca ggcaggagtg
     7561 aggcaagtct tgatagtcaa ggggaaagag atgcggaaaa tgaagccttg atcccttttt
     7621 cttctttttg tctgctatca gcgtagttag ccctctggta tgatgagtcc aactttgttt
     7681 tgctgtgtta tggaaatctc actatggtca ttaaggcgca aagcccggcg ggtttcgcgg
     7741 aagagtacat tattgaaagt atctggaata accgcttccc tcccgggact attttgcccg
     7801 cagaacgtga actttcagaa ttaattggcg taacgcgtac tacgttacgt gaagtgttac
     7861 agcgtctggc acgagatggc tggttgacca ttcaacatgg caagccgacg aaggtgaata
     7921 atttctggga aacttccggt ttaaatatcc ttgaaacact ggcgcgactg gatcacgaaa
     7981 gtgtgccgca gcttattgat aatttgctgt cggtgcgtac caatatttcc actattttta
     8041 ttcgcaccgc gtttcgtcag catcccgata aagcgcagga agtgctggct accgctaatg
     8101 aagtggccga tcacgccgat gcctttgccg agctggatta caacatattc cgcggcctgg
     8161 cgtttgcttc cggcaacccg atttacggtc tgatccttaa cgggatgaaa gggctgtata
     8221 cgcgcattgg tcgtcactat ttcgccaatc cggaagcgcg cagtctggcg ctgggcttct
     8281 accacaaact gtcggcgttg tgcagtgaag gcgcgcacga tcaggtgtac gaaacagtgc
     8341 gtcgctatgg gcatgagagt ggcgagattt ggcaccggat gcagaaaaat ctgccgggtg
     8401 atttagccat tcaggggcga taatcccttc cgtttaaaga gcaaccccct caaacgaggg
     8461 gttttttgtt gtttttacag atttcccatt cttggcgggc aacgttccag caactcgatg
     8521 ctgccgtctt cgttttgctg ttcgagcatc acatcaaatc cccacaggcg atgcacatgc
     8581 ttcaggactt ctttgcgccc ccgatccagc ggtgcgcgat tatgtggaat ataacgcagc
     8641 gtcagcgaac ggtcgccgcg caaatccacg ttccagatct gaatattcgg ctccagatta
     8701 cttaagttat attgcgacga taaccggtta cggatctccc gataaccttc ttcattatga
     8761 atagcggaaa tctccagata attatgccga tcatcatcca gcacggtgaa gaagcggaaa
     8821 tcacgcatca ctttcggtga cagaaactgg ctgataaaac tctcatcttt gaaatcacgc
     8881 atcgcgaaat gcagcgtttc cagccagtcg gaaccggcga tatccgggaa ccagtatttg
     8941 tcttcttccg ttggcgactg acaaatccgt ttaatatcct ggaacatggc gaacccgagg
     9001 gcatacgggt tgatgccgct gtaccacggg ctgttatagg ggggctggaa gaccacattg
     9061 gtgtggctgt gcaaaaactc cagcataaaa cgttccgtta ctttcccttc atcatacaga
     9121 tggttaagga tggtatagtg ccagaaggtc gcacagcctt cgttcatcac ctgagtctgt
     9181 ttttgcggat aaaaatactg gctcacctta cgcacaatac gcaggatttc acgctgccat
     9241 gattccagca gtggcgcatt tttctccata aaatagagca ggttttcttg tggttcggac
     9301 ggatagcggc gcgcttcagc aaccgttttc tcttcctcgc gcttcggcag ggtacgccag
     9361 agcatattga cctgactttg tagatactct tcgcgacttt tctgccgggc tttctcttct
     9421 tgcagcgaga ttttttgcgg gcgtttgtac cggtccacgc cgtagttcat cagcgcgtgg
     9481 cacgagtcca gaagccgttc tacttcatca acgccataac gttcttcgca ctcggtaata
     9541 tatttacggg caaaaatcag ataatcgaca atcgaactgg cgtcggtcca actgcggaat
     9601 aagcaattgt ttttaaagaa agagttatgt ccatagcagg cgtgtgccat caccagcgcc
     9661 tgcatagtaa tggtgttctc ttccatcaag taagcgatac acgggttaga gttaatgacg
     9721 atttcatagg ccagcccttg ctgaccgtgc ttatacagcc gttcagtctc gataaacttt
     9781 ttaccgaatg accagtgcgg atagttaatt ggcatgccga cgctggaata ggcatccatc
     9841 atctgttctg aggttatcac ttcaatctgg tgcgggtagg tatccagccg gtagagtttc
     9901 gccacccggt ctatctctgc cagataaaca tccagcaggt cgaacgtcca gtcgggtcca
     9961 tcgctcaaac gtgtggtgtc cttattcata gaatcgatcg tcgccatacg cgcacctcat
    10021 tgttgtcggc gctctctgtg tggagcacct catttcaagc atagaacacc tgttaaaaac
    10081 cgcgtcgccg gagaattttt ttctttgcga tttcttatta tcagagtgcc actaatccgc
    10141 ttctgaacgg aattttatgc tggataaaaa gggcgttcag caggagatac taaagacgcc
    10201 atattgccgc agagtcaggg agatgtgagt cagctcacca taaaaaagcc gtatgttgaa
    10261 taatattttc aactgagtta tcaagatgta attagattat tattctttta ctgtatctac
    10321 cgttatcgga gtggctatgc gagttgtcat actgggaagt ggtgtggtag gcgttgccag
    10381 cgcctggtac ttaaatcagg caggacatga ggtcaccgtc attgatcggg agccgggggc
    10441 cgccctggaa accagtgccg cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg
    10501 ggcagcacca ggtgtgcctt taaaagcgat taaatggatg ttccagcgcc atgcgccgct
    10561 ggcggttcgt ctcgacggta cgcagtttca gttgaaatgg atgtggcaaa tgttacgtaa
    10621 ctgcgacacc agccactaca tggaaaacaa agggcggatg gtgcgtctgg cggaatacag
    10681 ccgtgattgc ctgaaagcat tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg
    10741 tgggacgctg caactgttcc gtaccgaaca acagtatgaa aatgcgaccc gcgatatcgc
    10801 cgtgctggaa gatgccggcg taccgtatca gctgctggaa tccagccgcc tggcggaagt
    10861 ggatcccgcg ctggcagaag tagcgcacaa actgacgggc ggcctgcagt tacccaatga
    10921 tgaaaccgga gactgccagc tgtttaccca gaatctggcg cggatggcag agcaggcggg
    10981 ggttaaattc cgctttaata cgcccgttga ccaactgctt tgcgacggcg agcaaatcta
    11041 cggcgtgaag tttggcgatg aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc
    11101 ttactcgacg gcgatgctca aaggcattgt tgatattccg gtgtatccgc tgaaaggcta
    11161 ctcgctgact attccaattg cgcaggaaga tggtgcgcca gtatccacca ttcttgatga
    11221 aacctacaaa atcgccatta cccgtttcga taaccgcatt cgtgttggcg gaatggcgga
    11281 aattgttggt tttaacaccg agctgttgca accgcgtcgt gaaacgctgg aaatggtggt
    11341 tcgcgatctc tatccacgcg gcggtcatgt cgagcaggcg actttctgga cgggtttgcg
    11401 cccgatgacg ccagacggta cgccggttgt cgggagtaca cgctttaaaa atctgtggct
    11461 gaataccggt cacggcacgc tcggctggac gatggcttgc ggttccggtc agttgttaag
    11521 cgatctgctc tctggtcgca cgccagcgat cccatatgag gatctaagcg tagcgcgcta
    11581 cagccgtgga tttacgccat cacgtccggg ccatttacat ggcgcacaca gctaaggaaa
    11641 cgagatgacc cgtccgatac aggccagcct cgatctgcag gcattaaaac agaatctgtc
    11701 cattgtccgc caggccgcgc cgcacgcgcg cgtctggtcg gtggtaaaag cgaacgctta
    11761 cgggcatggt attgagcgta tctggagcgc gctcggggcc accgatggct ttgcattact
    11821 taacctggaa gaggcaataa cgttacgtga gcgcggctgg aaggggccga tcctgatgct
    11881 ggaaggattt ttccatgctc aggatctgga gatttatgac cagcaccgcc tgaccacctg
    11941 cgtacacagc aactggcagc tcaaagcact gcaaaatgcg cggctaaaag caccgttgga
    12001 tatttatctt aaagtgaaca gtgggatgaa tcggttgggc ttccagtccg atcgcgtgct
    12061 taccgtctgg cagcagttgc gggcaatggc gaatgttggc gaaatgaccc tgatgtcgca
    12121 ttttgctgaa gcggaacatc ctgatggaat ttccggcgcg atggcgcgta ttgagcaggc
    12181 ggcggagggg ctggagtgtc ggcgttcgtt gtccaattcg gcggcgactc tgtggcaccc
    12241 ggaagcgcat tttgactggg ttcggcctgg cattattttg tatggcgctt cgccgtccgg
    12301 tcagtggcgt gatatcgcca ataccggatt acgtccggtg atgacgctaa gcagtgagat
    12361 tattggtgtc cagacgctaa aagcgggcga gcgtgtgggc tacggcggtc gctatactgc
    12421 gcgcgatgaa cagcgaatcg gcattgtcgc cgcaggatac gccgacggtt atccgcgcca
    12481 tgcgcctacc ggtgctcctg ttttagtgga cggcgtgcgc accatgacgg tggggaccgt
    12541 ctcgatggat atgctggcgg ttgatttaac gccttgcccg caggcgggga ttggtacgcc
    12601 ggttgagctg tggggcaagg agatcaaaat tgatgatgtc gccgccgctg ccggaacggt
    12661 gggctatgag ttgatgtgcg cactggcgtt acgcgtcccg gttgtgacag tgtaacttgt
    12721 tgtaagccgg atcggaggca gcgtcttctg gttgcgaaaa aatcatccat ccggctggtc
    12781 agcaactgta gttgttaatg tgacagagcc attgcccatg atagtgtcca ttaaaagaat
    12841 ggacactatt tccccggaac ctgaactcgc cgcacaggcg ttctacataa aacgcttacg
    12901 cttcattgtt gactcggagt cgacttcaga taaatcacgc ttcacccttg atgcagattc
    12961 gggttcatgt tcagattgtt gattaacagg ctgcgagtcc tgttgtcttt ccagactcaa
    13021 caggcccaac tgttgatcca ggagctccag gtcctcctgt accttcacgc gctccaggag
    13081 ttccgggtca tccaggaact ccagatccat aaggtcctcc agctcctcga gtccttccag
    13141 gtcaacctag ccttccttgt gcaaaaagtc caaaaggtcc ttcaggccct gctgtttttt
    13201 caggccctcg acggctttga gcagcgtcgg taaattgtct ttgtcccacg ggttaccatt
    13261 acagtcaaac accttcaggc tctggggctg cggcacctgt accgttttaa acttgttttc
    13321 tccacagtag taaattatta gtttgggcgg cagccgtggt gaagagatca aatcgttact
    13381 agaacagtca aatactttca ggcgagtcaa gttctccagc atctcatcag gtattgagtt
    13441 aatattgttg ccggcacagg atagtttata caggtactgg ggcatcgacg gcggtaaaaa
    13501 ttgcaagaag ttttcagaac agttaatcac tctcaggtta gtcgacagct caggcagcca
    13561 gataatactg ttttcatagc acttcagtac catcagtttt gagggctgca ccttcggtaa
    13621 caagatcaag acgttatctg tacagtcaag caccagcagc cactttggca tcaccttcgg
    13681 taacgcggtc aacacgtttt tactgcagta aaattcaatc agatgtggag gcaaattctc
    13741 agggagtgaa gtcaatccca ggttgctaag attgagcata ctccgtcttg taatgagaca
    13801 ttccttcatt ctgcgcaatg cttctgcccg gttttcaatc cggcttgctg gcaggttttc
    13861 ttccaggata ttttccttga tttcttccag actttctttc aggtcttctt ggggaatttc
    13921 tgactgtttg ttttgctggt tttcttccag gatttcgtac aggttttttt gcaggttttc
    13981 ttgcaaacca tcctgctgta tagcccattc gcaccatttg gcgaccatca tgaactcgtt
    14041 atgttgctga ttcagacgag tccccaacgt gatgatggta ttgttgtcca cctgtatgtt
    14101 cactttgccc gcgatactga ggtcaatgat ctgccagggg gcatcacccc agctgaattt
    14161 ttgcgggctg agatacttca tgttctgcaa aatttgattc ttgagaagag ccgccctggc
    14221 gggcgtcgta cgcctgggtt aacggaagat gggggaatgg cgggggcgaa tgggcagatg
    14281 tctttaaagc tagccataat gctacgtttc cttgaaataa atatcctctg gcagcgccgg
    14341 agacgtgaat gtggtcaccg caaaggagcc acaggacgtc atttgcgctg atatcactac
    14401 tcatcgttga cttttttcct ggtcagaaat gccgtgtcgg gtctggtaca tgctgtatta
    14461 cggcctaggt agctctgacg tattaaagaa aatacgagag tgaacgaccc ggaaataatc
    14521 gttattaagc aaaggaataa ctataagaaa tcgctcatgc atccggtgct acaggcggat
    14581 gaaacgccta ttgtgtgggc tatgtcagtg cggcaggttg cgtgggggaa tatgttcagg
    14641 cgatgtcagg caggggcccc tgatggttga ccggtaatgc ggggctggtt tttattacct
    14701 gttacagtcg gaaaaagccc ttacgctgac tttcttgagc cggtgttttg agttcattag
    14761 ccagagcctg caatggtttg tcatccataa tttaaccttc ttctgatgtt gggatgagca
    14821 tatcaaaatc aggaaatgac aagtttatta caggctcagg ctgcgttcag taaagcatcg
    14881 catccggcga tagatgctgc attaaattta agattcagct tcctcttcag ccacccgaac
    14941 gccaatcttc aacacttcat tatcttcttt ctcggccacc gtccagatca tcccggcaaa
    15001 ctctacctgg tcaccgacaa ccggtgccgc gcctagcaac tgctggacaa tttcacccag
    15061 cgtttgctgt ttatcacgat attctcgccc gtcttccagg ccatatatca gcgcaacatc
    15121 agcatatttg gcgctggctt cgagaatgaa gtcaccaaag aagcgttgat ccagcgcgac
    15181 cggtggcgac tggctgaaca gtttaccgag cgccgggaga tcgcgttccc gaccaattac
    15241 acacaataca tcgccttcgc gcagtcgggt gctgccggta ggatgaagca actggttatc
    15301 acgaaacagg gctgcaatac gcgtctcttt tggcatatgc aaatcgcgca gtgccgcacc
    15361 tacacaccat ttatcagcac tcaattgata aacaaactgc tcccacggat tttccggatg
    15421 aatatccagg ccaacgcgtg acaccggacg tccgacgggc ggaaccacca ctttggcttt
    15481 tttcgccgcc cacgagagtg atgttccctg caagagcagt gaaaccagaa ccacaaagaa
    15541 ggcgacatta aagaacagtc gcgcattctc cagccccgcc atcatcggga acacggctag
    15601 gatgattggc accgcgccgc gtaatcccac ccagctgata aacacgcgct cacgcagatt
    15661 gaaaccgcgg aaagggagca atccagcaaa taccgaaagg ggacgggcga agaatatcat
    15721 ccatgccgac aaaatgagcg ccggaatggc aataggcagc agatcgcttg ggttaaccaa
    15781 tagccccagc accaggaaca tggcgatttg cgccagccag gcgaggccgt cgaaattttg
    15841 caggatgccg tagcggttgc gaatcggccg attacccagc agaaaaccgc acagataaac
    15901 agccagaata ccgctgcctt ccagcgccgt ggttaaagca aaaattaaaa taccgccgct
    15961 taatgccagc aatggatata atccggcagg cagggcgatg cgattaatca tttgcagcag
    16021 taaataacca ccgccaagac cgatgacaat cccgaggcca aattgctgca gaatatcgac
    16081 cacgaacatc caactgacac tgctttcatg ctgctggatc attgcaatca gggtaatcgt
    16141 cagaaagacc gccattggat cattactgcc ggattcaatt tccagcgtcg agccaacacg
    16201 ttcgttaagc cccttaccac ccagcagaga aaagaccgct gcagcatcgg tggagccgac
    16261 gatagcgccg attaataagc cttcaatcaa atcaagatta aacagccacg ccgccatcat
    16321 gccggttaaa ccagaggtga taagtacgcc cagcgtcgcc agcgacagcg ccggtcctaa
    16381 cgccacacga aaggaactgg cctgagtgcg catcccgccg tcgagcaaaa taatcgccag
    16441 cgccaggtta cttaccatgt aggcgaaggg gtaattatca aacgggatac cgccgacgcc
    16501 atcgactccc gccagcatgc cgatcgccag aaaaataacc agaataggaa tgccaagacg
    16561 ggaagaaaat gaactaagta atatactgct ggtgacaagg atggatccta agatgaaaag
    16621 gctaattatt gttgtggcat ccaatggtcg gttactcctg attacgctgt ctcttatata
    16681 aaccctacca tattagcggc agagacagcg ttttacttag ccctgaagcg tgattttttt
    16741 acattttaag aacaggatga ccgctaatcg tcaactgagc gccttcccgg gtgttattca
    16801 gaatggcatg tgcgcccaac ggcagcgtaa ccgtgcgttg ttcatgacca aaatcgaggc
    16861 cggtaatgag tggaatcgac aggcgggaac gcaaaaacgc gtacactgac tccaggttgt
    16921 aacctgcgtc ataatcattg ggcgtgctgc cgctaaagct accgagaata atcgccttct
    16981 gacgtggcaa aattcccgca tgatagagct gcaacagcat acgttcgacg cggaaagggt
    17041 gcacgttaat atcttccagc accagaatac cgttctcaat ttttggcatc cacggtgtac
    17101 caatcagtga aatcagcatc gcaagattgc ctccccacaa cgtgccttcg gtctgacatg
    17161 tcgggccttc cccttgccat tcaatggtga aggtttcatt gcgtaacgcc agccagaaat
    17221 ggtgctcggt aaaggcgttc agttcatccg caccaaagtt tgctaccagc atggggccgc
    17281 taaaggtgat gacattgcct tgcgccagaa gaccgcactg aatggcggta aaatcgctat
    17341 gtccgcaaat gagtaacggg tcatgttgtt ggcgagccac cagtgcctgc cagtcaatat
    17401 ctgccagtaa acgactggca ccgtaaccgc cgcgtacagc cagcacgatg gtgttggggg
    17461 tagtcagtct ggcaagggaa ttgagatctt ccagacgctc cgtttccgta cccgcaaaac
    17521 gctcacaacg acgggcaata acctcgacgt tattgacttg atgccccgcg tcggtaaggc
    17581 gctggatacc acgcagcgcg gcgtgctgtt taatgctgta acccgatggg gcaattaagt
    17641 gaaacagaga catggcaatt ccttgctgac aacagaaacg aaatgtatat catgccgctt
    17701 aggtgtgccg ttgtcacctc aacggcgatt ccaggctata aggatagaag aagtgaaatt
    17761 gagatggttt gcctttttga ttgtgttatt agcgggttgt tcatcaaagc atgactatac
    17821 gaacccgccg tggaacgcga aagttccggt gcaacgtgcg atgcagtgga tgccaataag
    17881 ccagaaagcc ggtgcagcct ggggcgtcga tccacaattg atcacggcga ttatcgctat
    17941 cgaatcgggt ggtaatccga acgcggtgag taaatcgaat gccattggtt tgatgcagtt
    18001 aaaagcttca acctccggac gtgatgttta tcgccgtatg ggctggagtg gtgagccgac
    18061 gaccagcgag ctgaaaaatc cagagcgtaa tatttcaatg ggggcggctt acctgaatat
    18121 tctggaaacc ggcccgctgg caggcattga agatccgaag gtactgcaat atgcgctggt
    18181 ggtgtcatac gctaacgggg caggtgcgct gctacggact ttctcgtcag atcggaaaaa
    18241 ggcgatcagc aaaatcaacg atttggatgc tgacgagttc ctcgatcacg tagcgcgaaa
    18301 tcatcctgcg ccgcaggctc cgcgctatat ctacaaactt gagcaggcac tggacgcgat
    18361 gtaaatcagt cgcgcacttt gtccgctttt tcccgggctt ctcgctcgag agagaaaata
    18421 atccgctgta attgccgctc caccgtcggg ctgacgttaa gaaaacggaa gctcagacgg
    18481 ggagtggtga tggtttcatt cttgccatca atcactttgc gctcgctggt ggagattaac
    18541 tgggcgtcaa agtgaaaaac accccattgc cccatgttga cttcaatttg agcgaagcgc
    18601 ataccttctt gtaattcggc aggctttgct gtttccagta atgcgcccat gccgcctaac
    18661 gacaaatcat acaagcggaa acgtaacgta ctgttatccg ccagtttggt ctggcaaaaa
    18721 taaggcggat ggagtggggc ggagatgcgg aaatatcggc gtcgttgtac aaaccataag
    18781 gtgggaggcg gtacggtaat aaatgccgga agctgcaagt attcactctg ctgtagttgt
    18841 tcaacagtaa actcgacttt cgcaccctga gtttcggcgg taatggtaat gtgctgtgcc
    18901 tttagcacgg cgatgttgtc ttcggcttga ctgccgaaat ccagcaccag tttatccggg
    18961 gttattgcca gtattttgct gatcagctgc ccgccattcc aactgagacg caaaggaatt
    19021 gcggctttgt gcaaatcgcg taacacgccc aggacggcta acggattttg tttcaggaac
    19081 tgctcatggt aatgactcac gcggaaaaac tcctgactga caaactgtct ttggtttatc
    19141 ggtcatagtt aacaaaactt aatacaaatg cgtgaatatt ttttacatgt tgttcttaaa
    19201 tcagccgaca aacgcccagc atcgatcccg cccttgccta tacttagagc gttgacgtaa
    19261 gcatttcttg cgaccgattc atcgaaacga gggcctgaac atgggaatta ttgcctggat
    19321 tatttttggc ctgatagccg gcattatcgg caagctaatc atgccggggc gtgatggtgg
    19381 tggatttttc ctgacctgta ttctcgggat agtcggtgcg gtggtcggcg gctggctggc
    19441 gaccatgttt ggcattgacg gctccatcag cggttttaat ttgcacagct tcctggtggc
    19501 ggtggtgggc gctatcctgg tgctgggcgt attccgcctc ctgcgaagag aataagattt
    19561 tcataaggcg gatagcgata cagatgccgc tatccgcttt cacatcagaa cgtatactcg
    19621 acacctgccc agaaattgcg accttcttca ataaaccctt cactgtaggc atattgggta
    19681 tcaaacaggt tattgaccga cgcattgacg ctgaagccat gacctaaggt gtaatcggct
    19741 cgaatgtggg tcaccgcaaa accggcggct ttttgtgaac cgtcactgtt gctgtagctg
    19801 gaggaacgcg cctcttccga cagcgttacg cttaacggct cccatggttt gagtgtcatc
    19861 catgcggtca ttgtctgcgt tggaagatcg gttatcttgc cgatgtcttt gcgtttggcg
    19921 tcagcgtgga tcagagcgta gctcaatcct acatccagta tattgctgat tttcccctta
    19981 ataccggcat ccagaccgct gtaatccacc gtgccgctgt tctgattttg aatggtatcg
    20041 gcattgatat tgtgcgagag gatggcatca ctcacccggt tatagtaaac gctgacctca
    20101 aatccccagt cgcgcgtgaa ggcaccattc caggttaaat ccaccccgcg agcgcgttcc
    20161 ggcttgagct gcgggttaac aatcgctatc tggttatacg caggtttgga tgtggtatag
    20221 cgttctttca gcgtcggaaa gcgtgtgcgg tcatagtacg aaagcgccag agtgtcttca
    20281 ttggcaaagt ggtatttccc catcacctgc cagttaaaag ctgactgatt gttgtcgtca
    20341 taatgggtga tgctgccatc tttctcgtgt tttttcgctt ctacgctatc gcgccagtca
    20401 tagctgattc ccgccacgac atcgacatta tcagcagcag cccattgata ttcactggcg
    20461 agcgaccagg tacgatcttc atagcgatcg taagcggcgt gcggcgcacc tttttcccgg
    20521 tgtacgtcat ctttccagtt aacggcaaac gacagcagat cgttttcacg cacatcggct
    20581 gccagttgta atccggcacc gtcgctgtaa tcggaataat ggctgtagct gcctttttta
    20641 tttttcaaat cagccagcga gttgtacatc atcagcgtat tttcaaaggt gtcgcgatac
    20701 agccgacttt tcagggtaaa acggtcgttt agttgggtcg ctccctgata ataaaaactt
    20761 tctttgtcat actctggcca ctgccagtag cgtgattttt gaccacaatt tccgctgtat
    20821 ggcgggttat ctttttcacc atcctgctta atgtaagtca gcgtgtattc atcgttttcg
    20881 cgcggtgtaa aaccgagctt cacaatgccg cgtttatcat cagccgatga attaatcatc
    20941 ttgccgtgtt tgcctgcaat atcattattt acaccatgcg gcaggccgag aaaatcctgc
    21001 tttagctggc taccgctgac ttgcaaatac ccaagatcgc tgctggcggc aaatgaagca
    21061 tgcatatcgt aggcattgtc ctgactacgg ctccatccct ggcgatatcc cagacttgct
    21121 tcttcagggt ggtgatatta atggctccgc ccatctgatt aggcccatga agcagcgacg
    21181 aatacccttt ggaaacttcg actgccccca gattgttggt cagaatccgc gccagatcga
    21241 ggttgccgtc ataggggaca taaatgggca caccgtcgaa atagaccggc acctgccgac
    21301 tatcaaagcc acgaactttt acctgttctt cgttgcggct gcctgacttt tgcagcacca
    21361 cgccggggac gacacttaat gcctgagcga cattctgttt atccaaggcc ttcatggtgg
    21421 gttgatcgag aacggtggtc gtcgtcgatg ataccggact ggaccatacg gtcagcgtgt
    21481 cgctctcctg agccgctacg gcctgctggg taaaggcaag tgataatgct gtgcagagat
    21541 aacgtttttt gagtctcata gaataaattc cgtttaacaa agaattaaat agaggctgtg
    21601 ggttgcagac tgactttaat gtcagctggt gccgcgtaat aaggcgcgga ggtaataaaa
    21661 aggcgaatgc cgcagtcgag gtaatttttg agtgtcgtca gattaattcc gccggtcaga
    21721 gccagcgtgc agtgaggagc cagcgacggc gctatttgcg caatttcagt tgcctgttgc
    21781 ggactaaatt tgtcgagctg aagtacgtct ggttgtgcgc gtaacgcggc gatagcctct
    21841 ttcggcgtgt cggcttcaac aacaattttc ttctcgggtg cgtggcgacg taactgagcg
    21901 attgcgcctg accagccctg attgtcatga agaaaatggc ggtggttggc aaacaataat
    21961 atggtttccg cacatccggc acgatgaatt agtcctccgg cagccagaat tgcctgcgaa
    22021 gccagtaagc gggtgcccgg aattgctttt cgggtgcagg cgatattgcc gtcaggataa
    22081 cgttcacgaa gcaatgccag catttgagcg agataatcag aaacaccgca actccactcc
    22141 agcacattct ggactgcctt ccatccctga tgaagtgctg cggcattacc ctgcgcacgg
    22201 attagccgtt gacccgcgtt cgcttgtgaa ccgtcgctga ccgcgtcatc aatagttaat
    22261 cccagcgtag ttaacatctt acacgcgaca gaaacgccgc tgacgcagcc gccctgacgg
    22321 tgaaaaaacg ctatatagcc aggctggtgt ccaatattta atgctcgcgt ggtcaggtca
    22381 cctccctgga tatcttccag cagtaacgca tcgatttgtg cctgagaaag gaaaatcatc
    22441 acagtgcctc ctgcggtacg ctatcccacc agatgagcgc ccagtcgcgg ctggcagggg
    22501 cgagggcacg agcatcttgt tgttgatacc agtgatataa ccgctcacgc tcatcgtcgt
    22561 taagcgcgcc aagactccag ctgacgttct gctcaaaacg ttcccaggtg ctgttatcct
    22621 gttggcaatt ttgtccacgg ataaaatcaa cgtgggcata aattcccatc tgatacagga
    22681 cgttgagcgc aaagatatag ttgggtaatt cgatcacttc ccgaccagcc gcccgctgaa
    22741 tggctggaga gacgaacgag gtgctgacca gatgagttgt atagacgcga agccgtgcct
    22801 gattattcag tttgctcatt gcatggcgca tatcagccac cagagtggag cgggaagcca
    22861 cggcaatatc gcagcgtggc aggtcactcc agtcctcttc ccaggcgcgc tggatccagt
    22921 ggacattgtc agccttcagt gcggcggcgc ggcgggcggc aacgttcagc atcccctgac
    22981 tgtaatcaac gccataaacc gtagtgagtt tgtcagccag cgccagcgat acggtgcctg
    23041 gtccgcagcc catatcgaat aaggtctgag cgccttgcag gtcaatttta gcgatcagtt
    23101 gttgcaggta gctgtccgtt ggcgaggcgc aattttccgc cattttctcc gcccgttgat
    23161 cccagtgatc gggcgttttt tctgtgcggt gagccagttt gagttgctgc agataaaggt
    23221 ctgcaaaatc aatatcatca atgagcatga ttgttcctta gcggctaatt gcagagaggt
    23281 gatgatgaat ttggtcggca gaaacccgat acagggccgc cagtttgttc gttgttaact
    23341 gttcggtcgg taagccttgt gttactcgtc catctggctc tacttggata atgctgtcgg
    23401 caatggcgtt ggcatggagc gtatggtgag tcgacattaa cattgtcatg ccgttatttt
    23461 tgagctgcgc cagcgtgtcc agaagttgga tctgatgacc gaaatcgagg ctggaggcgg
    23521 gctcatccag taacaacaag cgcggttgtt ggacgagtgc acgggcgatt aaagccagct
    23581 gacgttcacc gccgctaagc gtattccagc gtcgtgtcgc cagatgcaaa atgcccagct
    23641 tttccagttg ctccgtggct ttcaggcgtt cttgcttacc gggaacggaa aaagcgccta
    23701 tggtgggggc caggcccatc aacaccatat ccagcacact gaaagcaaaa ataccgtcgt
    23761 gcgcctgcgg cacccaggca atagcctgtg cgcgctggcg atggctgagt gtctgcacag
    23821 agaggctatc cagtaggatc tcgcctttta ataacggtat aacgccaaga atggagcgca
    23881 tgagtgtggt tttgccgcaa ccattcgcgc caagcagaca acaaattgtt ccttgcgaca
    23941 gggtgaagga cacgtcgcgt agcactggat ggcgggagta acccagagac gcatgacgaa
    24001 cggcaagcaa agtcactggc gacctccgcg taacaacaaa agcaggaaaa acggtgcgcc
    24061 aacaaaagcg gtcagaatgc caagtgggat ttctgttgtt ccaatgctgc gcgccagcgt
    24121 atcggtaagc aggagcagaa tagcgccagt acacattgcc ataggcaaca gctgctgatg
    24181 gttatgaccg gtcaacaagc gggcaatgtg cggaacgacc agaccgatcc aaccaattat
    24241 gcccgctatt gctaccgtgc tggcggtgat tagtgtggcg cagacaatca atccaaagcg
    24301 cagacgcgtc acgttcagtc ccagcgaacg ggcttcgtcg tcggaaagcg tgagtaaatt
    24361 catgcgccag cggagaaaaa acaggggcaa ggagcctatg agaatgatcg gtgcagcata
    24421 gcagagatcg cgtaaggtca cggtggacag tccccccagc aatcagaagg taattgacgg
    24481 taactgggtg taaggatcgg ccagcgtttt aattagcgat atccccgctc cgcacaacgt
    24541 tcctaacgca atgcctacca gcaccagggt taagatcgga tcgtggcggg tcacccgacg
    24601 cgtaatcaac cagacgcccg cgaccaccat cagcccgccg caaaatgcgt acagctggat
    24661 gtaaacaatg ggtaatcccc acaggattgc cgcgcaggca ccaagtccgg caccggcgga
    24721 gacgcctaaa atatccggtg aaactaaggg attacgcaac attccctgat aggtggttcc
    24781 ggctcctgcc agcgctgcgc ccagtaacag cgcagcaaga atacgcggca cgcggatttg
    24841 ccagaagaca atttgttcct gcacagactc attttcttga cgaagaatta gcgccagcag
    24901 ttgctgcata tcgagatgat aagccccact tacggcggca aaagtgatac tgatagctgc
    24961 cagcagcaaa atacatccgt ttacgattct cattagctcg ccaccaattt ttgatagtcg
    25021 gcatcactga gtgaacaatg ccagaacagt tgggcgtaat gctgcatgtc acttttaaac
    25081 tggcgattga tggcgggatc cagccaggcg tgaagtctgc gtaatcccag aagacggttg
    25141 attcccggcg gggcatcgag ccagccaaag ggcaagccac ttaaaaataa gatgcgttgt
    25201 tccgcaacgg ctttcacgcc ctgccagaga ggatcacgac gaataaaatc tgcagtaacg
    25261 gcctcctgaa ccagaataat atccggctgc cagcgcagga gattttccat ggaaacctgt
    25321 gtcaggccgt ggcgatcggc tatttgcgcg acgttgtgca aacctaataa ttccgccgcc
    25381 tcggtatgca acgaaccctg taagcccgtt tccagccctc ggggaccgcg cgcagcataa
    25441 aagcggaggt tagcggcggg tgaggtggcg aatgcctgag cttcaccaac gaagtggctg
    25501 gcgagattgg cttgttctgc ggcgcggtgc tcttcgccta acgttttgcc aagcgttgtt
    25561 aactgttctg ctgattgcgc cagttttccg ttaagcaata accaggggat ttgtgtctgt
    25621 tcgctaacct gccgtgcctg ggagatcaag gtttcatccg tattgccgca atcaacgacc
    25681 aaatcgggat gtaacgccat aagcccttcc agcgatagtg tgctggcgcg cccggcgagt
    25741 cgtcccagcc tgggtaactg gcgaatgtgc tctggcaatg gaattaatgc ctgacgggca
    25801 aaatcaaaag aggaaaaacc gaccattttt tctggcgcga cagccagcaa gagcaaatcg
    25861 gcgggcgcgc ctgcgctgac gatgcgtgta atgttttggg cttcgggaag agattgcgaa
    25921 gcaaaacgat taactgtttg tgaaaaggac ggtagagact gcaggagcag ggtagatgca
    25981 agtgcctgcg caaacgtccg tctggttatg ggcatggtga acatcctgaa aagtaaaatc
    26041 gatatatact ttcatatata tcgataaaaa tcatgttcaa tatcaaagtt tagctattga
    26101 aaacatgaag atgtgacgac ttccgcaagc ctgttgaccc tctctatcat taaaaatgta
    26161 tttatcaggt ggctaatatt atttaaatca tttaaggcta aattatttaa atatgaatga
    26221 cattattgtt tttttatcgt ccacggttac gcgacgttga agttacttgt tttataatga
    26281 gcgatttttg atatctcgcc gagaaaatta tcagcattct ctcgtttcag tgtcgtgaat
    26341 atactgttaa cggaggtgaa aagaatgctg ggggttatac aaaacctatg ttgttgtcgt
    26401 ccggattcac cgcttgctga tagtaagcag cgtaaccaga agttaccgca acaagacttt
    26461 gaggatttta agaacgacgt tattaatgtc attaacaaca atgaagatat ttattttctt
    26521 agagagagta atttactgaa tgcgtttatt cggacgtgca aaaaaagaga tgataacagt
    26581 tacggaaata tgatatttac gctttatttt gataacgata tctatgggaa cagaattatc
    26641 aaagcatcgc tgaacggcgg agagttgcag ttattaagca cagggtgtag caatgataac
    26701 attctc
//
LOCUS       DAJTUM010000054        25870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTUM010000054 DAJTUM010000000
VERSION     DAJTUM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..221)
                     /locus_tag="OO471_002389"
     CDS             complement(<1..221)
                     /locus_tag="OO471_002389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6243628.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSR"
     gene            complement(354..659)
                     /locus_tag="OO471_002390"
                     /pseudo
     CDS             complement(354..659)
                     /locus_tag="OO471_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            727..1342
                     /gene="tnpA"
                     /locus_tag="OO471_002391"
                     /pseudo
     CDS             727..1342
                     /gene="tnpA"
                     /locus_tag="OO471_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1670..2752
                     /locus_tag="OO471_002392"
     CDS             1670..2752
                     /locus_tag="OO471_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6243629.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3241..4039)
                     /locus_tag="OO471_002393"
                     /pseudo
     CDS             complement(3241..4039)
                     /locus_tag="OO471_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4049..4600)
                     /gene="ybcL"
                     /locus_tag="OO471_002394"
     CDS             complement(4049..4600)
                     /gene="ybcL"
                     /locus_tag="OO471_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6243630.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4769..5101)
                     /gene="emrE"
                     /locus_tag="OO471_002395"
     CDS             complement(4769..5101)
                     /gene="emrE"
                     /locus_tag="OO471_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6243631.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5446..5760)
                     /gene="fliE"
                     /locus_tag="OO471_002396"
     CDS             complement(5446..5760)
                     /gene="fliE"
                     /locus_tag="OO471_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6243632.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5974..7633
                     /gene="fliF"
                     /locus_tag="OO471_002397"
                     /pseudo
     CDS             5974..7633
                     /gene="fliF"
                     /locus_tag="OO471_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7626..8621
                     /gene="fliG"
                     /locus_tag="OO471_002398"
     CDS             7626..8621
                     /gene="fliG"
                     /locus_tag="OO471_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6243633.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8614..9300
                     /gene="fliH"
                     /locus_tag="OO471_002399"
     CDS             8614..9300
                     /gene="fliH"
                     /locus_tag="OO471_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6243634.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9300..10673
                     /gene="fliI"
                     /locus_tag="OO471_002400"
     CDS             9300..10673
                     /gene="fliI"
                     /locus_tag="OO471_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6243635.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10695..11036
                     /gene="fliJ"
                     /locus_tag="OO471_002401"
                     /pseudo
     CDS             10695..11036
                     /gene="fliJ"
                     /locus_tag="OO471_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11033..12160
                     /gene="fliK"
                     /locus_tag="OO471_002402"
     CDS             11033..12160
                     /gene="fliK"
                     /locus_tag="OO471_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6243636.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12265..12729
                     /gene="fliL"
                     /locus_tag="OO471_002403"
     CDS             12265..12729
                     /gene="fliL"
                     /locus_tag="OO471_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6243637.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12734..13738
                     /gene="fliM"
                     /locus_tag="OO471_002404"
     CDS             12734..13738
                     /gene="fliM"
                     /locus_tag="OO471_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6243638.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13735..14148
                     /gene="fliN"
                     /locus_tag="OO471_002405"
     CDS             13735..14148
                     /gene="fliN"
                     /locus_tag="OO471_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6243639.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14151..14516
                     /gene="fliO"
                     /locus_tag="OO471_002406"
     CDS             14151..14516
                     /gene="fliO"
                     /locus_tag="OO471_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6243640.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14516..15281
                     /gene="fliP"
                     /locus_tag="OO471_002407"
                     /pseudo
     CDS             14516..15281
                     /gene="fliP"
                     /locus_tag="OO471_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15291..15560
                     /gene="fliQ"
                     /locus_tag="OO471_002408"
     CDS             15291..15560
                     /gene="fliQ"
                     /locus_tag="OO471_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6243641.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15569..16354
                     /gene="fliR"
                     /locus_tag="OO471_002409"
     CDS             15569..16354
                     /gene="fliR"
                     /locus_tag="OO471_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6243642.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16639..17262
                     /gene="rcsA"
                     /locus_tag="OO471_002410"
     CDS             16639..17262
                     /gene="rcsA"
                     /locus_tag="OO471_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6243643.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17306..17494)
                     /gene="dsrB"
                     /locus_tag="OO471_002411"
     CDS             complement(17306..17494)
                     /gene="dsrB"
                     /locus_tag="OO471_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6243644.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17657..17884
                     /gene="yodD"
                     /locus_tag="OO471_002412"
     CDS             17657..17884
                     /gene="yodD"
                     /locus_tag="OO471_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6243645.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18182..18997
                     /locus_tag="OO471_002413"
                     /pseudo
     CDS             18182..18997
                     /locus_tag="OO471_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18994..20688)
                     /gene="yedQ"
                     /locus_tag="OO471_002414"
                     /pseudo
     CDS             complement(18994..20688)
                     /gene="yedQ"
                     /locus_tag="OO471_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20859..21041)
                     /locus_tag="OO471_002415"
     CDS             complement(20859..21041)
                     /locus_tag="OO471_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6243646.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21120..22037)
                     /locus_tag="OO471_002416"
     CDS             complement(21120..22037)
                     /locus_tag="OO471_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6243647.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22210..23130
                     /gene="yedA"
                     /locus_tag="OO471_002417"
     CDS             22210..23130
                     /gene="yedA"
                     /locus_tag="OO471_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6243648.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23119..23589)
                     /gene="vsr"
                     /locus_tag="OO471_002418"
     CDS             complement(23119..23589)
                     /gene="vsr"
                     /locus_tag="OO471_002418"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6243649.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23570..24988)
                     /gene="dcm"
                     /locus_tag="OO471_002419"
     CDS             complement(23570..24988)
                     /gene="dcm"
                     /locus_tag="OO471_002419"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6243650.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25055..25595)
                     /locus_tag="OO471_002420"
                     /pseudo
     CDS             complement(25055..25595)
                     /locus_tag="OO471_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25635..>25870)
                     /locus_tag="OO471_002421"
     CDS             complement(25635..>25870)
                     /locus_tag="OO471_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243651.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6520 a         6636 c         6374 g         6340 t
ORIGIN      
        1 cggcttaact gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa actgtttaca
       61 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
      121 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
      181 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
      241 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg
      301 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
      361 aacgccgagc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
      421 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
      481 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
      541 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
      601 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
      661 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
      721 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
      781 gacgcgacag aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa
      841 ctggttgaaa tggatggcga accagatcac gtccatctgt taattaacta tccccccaaa
      901 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
      961 acgggatcga ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
     1021 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
     1081 acagcaaaca cctggtcaga tgaaaaaacg agccttatat ccccgccctg aaggacgggc
     1141 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtacg
     1201 atgccccgta catgctccat cacatcctca acatcaaagt attagagcct tgcaaaccat
     1261 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
     1321 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
     1381 atgcaaataa tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt
     1441 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
     1501 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
     1561 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
     1621 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
     1681 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
     1741 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
     1801 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
     1861 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
     1921 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
     1981 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
     2041 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
     2101 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
     2161 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
     2221 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
     2281 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
     2341 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
     2401 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
     2461 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
     2521 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
     2581 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
     2641 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
     2701 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
     2761 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc tctcaagcca
     2821 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
     2881 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
     2941 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
     3001 cctcaacctc ttgtaaattt cccactcttg agctccacat agcccaaacg ccggatgcaa
     3061 aaaaaatcat acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
     3121 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
     3181 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
     3241 ttactctctg gacgcttttc ttagctctct tggtgttata ttaaaacgat tatggaacct
     3301 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
     3361 tactgtcgtt tgtatagcag gacaacgcat tatttaacct cacgtcgtcc aatatacttt
     3421 gaaaacttat tccctcgctt gccagacggc gatgcaatgt ggaaacagaa atgtagagat
     3481 atctcgctac cttgtttgct gtccattttg ttctaggttc ggatagcatt aggttataac
     3541 aacgacttat taatgattgt ttactatctg ataaaagtaa acaattaaca tgatgtgctc
     3601 ctaatgaaag tagaacgccc attgctaagt gttcctgaat ttgcgttgag aagcctcggg
     3661 aaatagatgt ttttagttgc tcccaacaat atattaactc ggggttctga ggtaagcaga
     3721 aacctgtcgt gttacgaatt tgatcagtta tcgcataaaa tttttgaaac ttttcaatta
     3781 gatcaatggg ataataaagc atttctgcaa gataaagacc tgcttctgga taattcacaa
     3841 tataaaattc atatccacag ggaaataata ttatctgatt attatcaaca gttagactat
     3901 gcgtctccca attgataact ttttttccct gacggatacg acataaagct ggcataagag
     3961 gcttaaccct gtgaatctca tgatgtttat gcatccgtat ttcttcaatc tttaggttag
     4021 acttatctct tgccagcata ctcttaccct attttatctc ataaactggt gttatctcag
     4081 cggttgcaat tttattggca ttaagcatat aaccaactaa cgctccgctg gaattagaat
     4141 ccacaggaat cttatcagtt tttagagccc atactttaaa ctggtaatga tggggtttat
     4201 ctcctttggg aggacatgct ccaccaaatc ctgcataacc aaaatcattt cggccttgaa
     4261 cagcaccagt cggaagtttt gtaccatcac gtcttcctgc atcagtgggc aaatatgtta
     4321 cagttgctgg aatattagca acagtccagt gccaccatcc actgcctgta ggagcatctg
     4381 gatcatacac agttacggca aagcttttgg tgccttcagg agcaccagac caggttaatg
     4441 agggcgatgt attaccacct tcacacccaa atccagaaaa tacatgagac gttgtaagtt
     4501 gctctcctgt ttttatttca ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg
     4561 ttatgaatgc caatacactt gaaacgataa gttttttcat aaaaacctca ttgttgtgac
     4621 ctatcgttat tttatttgat gtgccttgat ctcattatgc ataaaggcgc aatgatcgcg
     4681 caaatgcaat cataattata aaggtgcccc gaacattagc cataatatgc acaaatcaga
     4741 ttcctgttat ctcaaggaga attatttttt aatgcggtgc acttcgtgac aacaagttaa
     4801 tgactaacac accggcacaa atcaacatca tgcctatgat agctggcagg tccagtcgtt
     4861 gaccaaaaaa tccccaggaa agcaaactaa tcagaacgat accgactcct gaccagatag
     4921 cataagcaat ccctgtaggt atataagcca gcgtctgagc taataaccag aatgatgcac
     4981 aatagcaaat aattgtgcca acagagggcc ataaccgtgt aaacccctct gaaaacttca
     5041 ttaaggttgt accaatgacc tctgcaagta ttgcgccacc aagataaata taagggttca
     5101 tagcatattc tttcctgttc atactggaga gaattgtact acagtttgaa cacaactcac
     5161 cgttttcatc ctggtgtatg tgtatccaag gggctctggc gtggcaagag ataaaggcag
     5221 gaacaataat gatcagaagg aaccccaggc taacgaatga gttcactctt cctgggagca
     5281 aagcgaagac aaaacatgtt atcgcgttta cggatcgtgg atggcagaaa acaaccagga
     5341 tcaggtatcc attttaaacc agaaattgag tgagtttgcc ccatccatgc cccacgctgt
     5401 gggatcggat ataattaaac aagcatagtc aacaggttac acaacctaca cctgcatgga
     5461 catcacttcc tgatacgccg ccaccagctt attacgcacc tgaatcccca tttgcataga
     5521 aactgaggct ttttgcatat cggtcatcac atcgtttagc gccacgccgg gttcaccgag
     5581 ggtgaatttt tctgcctgcg tgcgggcgac tgtttgtgta tcgcttatgc gatcgagcgc
     5641 ggcgtgcagc tgcccggcaa aactaatggt cggttgcggc agtgattcct gcgcacgcgc
     5701 actcatcgcc gtagcctgta actggctgat aaccccttca atcccctgta tcgctgacat
     5761 tttcgtctcc cggataattt ctggtaacaa agcctaccag taagtcaata agacaaaggc
     5821 gctaaatagc aacaaaaaaa ctggtttatt ggcggataga aaaaacgaaa gcacaaataa
     5881 tgggagcgtc aatttttcga gtgtgctgac ccgagagtga gtcttgttcc actttgccaa
     5941 taacgccgtc cataatcagc cacgaagtgc gcgatgaatg cgactgcagc ccagacaaaa
     6001 tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttaccggt
     6061 tccgcggcag tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc
     6121 acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa
     6181 atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa
     6241 gttcacgaac tgcgcctggc gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa
     6301 ctgctcgatc aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg
     6361 gcgctggaag gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc
     6421 gtacatctgg caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca
     6481 tcggtgacgg taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgctatt
     6541 gtacatctgg tttccagcgc ggttgctggt ctgccgcctg gaaacgtcac gctggtggat
     6601 cagggcgggc atctgttaac ccagtccaat accagcgggc gcgatcttaa tgacgctcag
     6661 ttgaaatatg ccagcgatgt cgaaggccgt attcagcagc gtattgaagc gatcctgtca
     6721 cctattgttg gtaacggtaa tattcacgcc taggtcacgg cgcaactgga cttcgccagt
     6781 aaagaacaaa cggaagaaca gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt
     6841 tcacgccagc ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg
     6901 gcgttgtcga atcaaccggc acctgcgaat aacgcgccaa tcagcacgcc tccgacaaat
     6961 caaaataacc gccagcagca ggcgagcacc accagcaata gtgggccgcg tagcacacag
     7021 cggaatgaaa ccagtaacta cgaagtcgat cgcaccattc gtcataccaa aatgaacgtg
     7081 ggcgatgtgc aacgtctgtc agtcgcggta gtggtgaatt acaaaacctt gccagacggt
     7141 aaaccattgc ctctcagcaa cgaacagatg aagcaaattg aagctctgac ccgcgaggcg
     7201 atgggctttt ctgaaaaacg cggtgattcg ctcaatgttg ttaactcgcc gttcaatagc
     7261 agtgacgaaa gcggcggagc actgccgttt tggcaacagc aagtgtttat cgatcagttg
     7321 ctggctgccg gtcgctggtt gctggttgct ggtactgctg gtggcgtggt tgctgtggcg
     7381 gaaagcggta cgcccgcagc taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca
     7441 ggcgcaggcc cgcgaggaag tggaagatgc ggtggaagtc cgtctgagca aagacgaaca
     7501 actccaacaa cggcgcgcta accaacgact gggggcagaa gtcatgagcc agcgtatccg
     7561 tgaaatgtct gataacgatc cgcgcgtggt ggcgctggtc attcgccagt ggataaataa
     7621 cgatcatgag taacctgaca ggcaccgata aaagcgtcat cctgctgatg accattggcg
     7681 aagaccgggc ggcagaggtg ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg
     7741 ctgcaatggc gaacgtcacg cagatctcca acaagcagct aaccgatgtg ctggcggagt
     7801 ttgagcaaga agctgaacag tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt
     7861 cggtattggt caaagctctg ggtgaagaac gtgccgccag cctgctggaa gatattctcg
     7921 aaacccgcga taccgccagc ggtattgaaa cgctcaactt tatggagccg cagagcgccg
     7981 ccgatctgat tcgcgatgag catccgcaaa ttatcgccac cattctggtg catctgaggc
     8041 gcgcccaggc cgccgatatt ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt
     8101 tgcgtatcgc cacctttggc ggcgtgcagc cagccgcgct ggccgaactg accgaagtac
     8161 tgaatggctt gctcgacggt cagaatctca agcgcagcaa aatgggcggc gtgagaacgg
     8221 cagccgaaat tatcaacctg atgaaaacgc agcaggaaga agccgttatt accgccgtgc
     8281 gtgaattcga cggcgagctg gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc
     8341 tggtggatgt cgatgatcgc agcattcagc gtctgttgca ggaagtggat tccgaatcgc
     8401 tgctgatcgc gctgaaaggg gccgagcagc cactgcgcga gaaattcctg cgcaatatgt
     8461 cgcagcgtgc cgccgatatt ctgcgcgacg atctcgccaa gcgtggtccg gtgcgtctgt
     8521 cgcaggtcga aaacgaacag aaagcgattc tgctgattgt gcgccgtctt gccgaaactg
     8581 gcgagatggt gattggcagc ggcgaggata cctatgtctg ataatctgcc gtggaaaacc
     8641 tggacgccgg acgatctcgc gccaccaccg gcagagtttg tgccaatggt cgagtcggaa
     8701 gaaaccatta ttgaagaggc cgaacccagc cttgagcagc aactggcgca actgcaaatg
     8761 caggcccacg agcagggtta tcaggcgggg attgccgaag gtcgccagca aggtcacgaa
     8821 cagggctatc aggaaggact ggcccagggg ctggaacaag gtctggcaga ggcgaaggct
     8881 caacaagcgc caattcatgc ccggatgcag caactggtca gcgaatttca aactaccctt
     8941 gatgcgcttg atagtgtgat agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt
     9001 caggtgatcg gtcagacacc aacggtggat aactcggcac tgatcaaaca gatccaacag
     9061 ttgttgcagc aagaaccgtt attcagcggg aaaccacagc tgcgcgtaca cccggatgat
     9121 ctgcaacgtg tggatgatat gctcggcgca actttaagtt tgcatggctg gcgcttgcgg
     9181 ggcgatccca ccctccaccc tggcggatgt aaagtctccg ccgatgaagg cgatctcgac
     9241 gccagtgtcg ccactcgctg gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa
     9301 tgaccacgcg cctgactcgc tggctaacca cgctggataa ctttgaagcc aaaatggcgc
     9361 agttgcctgc ggtacgtcgc tacgggcgat taacccgcgc taccgggctg gtgctggaag
     9421 ccaccggatt acaattgccg ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg
     9481 aaacgcacga agtagaaagc gaagtcgttg gctttaacgg tcaacggctg tttttaatgc
     9541 cgctggagga agtggaaggt gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg
     9601 cagaagggct gcaaagcggc aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc
     9661 tggacggtag cggtaaaccg ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg
     9721 cgctgattac cccgccattt aacccgttgc aacgtacacc gattgaacat gtgctggaca
     9781 ccggcgtgcg cccaatcaat gccctgctta ccgtcggacg cgggcagcgt atggggctgt
     9841 ttgccgggtc gggcgttggt aaaagtgtgc tgctggggat gatggcacgc tacacccgcg
     9901 ccgatgtcat tgtcgtgggt ttgattggtg aacgtgggcg cgaagtaaaa gattttattg
     9961 agaatatcct cggtgccgaa ggacgtgcac gctcagtggt gattgccgct ccggcggatg
    10021 tttctccgct cctgcgaatg caaggtgccg cctatgccac gcgcattgcc gaagattttc
    10081 gcgatcgtgg tcagcatgtg ttgctgatta tggactccct cacccgctac gcgatggccc
    10141 agcgtgaaat tgcgctggcg attggcgaac cacctgccac taaaggttat ccaccgtcgg
    10201 tgtttgccaa attaccggca ctggtcgagc gtgccggtaa tggcattagc ggcggcggct
    10261 cgattaccgc gttttatacc gtgctcaccg aaggcgatga ccagcaggac cccattgctg
    10321 actccgcgcg ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg
    10381 ggcactatcc ggctatcgat attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca
    10441 gtgagcaaca ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta
    10501 accgcgatct ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag
    10561 ccatcgccct gtggccgcag ctggagggct atttgcaaca aggcattttt gaacgcgcgg
    10621 actgggaagc gtctctccag gggctggagc gtattttccc gacagtgtca taacccagga
    10681 gataacggca gatggcggaa gaacagctca aaatgttgat cgattatcag aatgaatatc
    10741 gcaataacct caatagcgat atgagtgccg ggatgaccag caaccgctgg atcaactatc
    10801 agcagtttat ccagacgctg gaaaaagcca ttactcagca tcgccagcaa cttaatcagt
    10861 ggacgcagaa agttgacatt gccctgaaca gctggcgaga aaagaaacaa cgtttgcagg
    10921 cctggcagac actgcaggaa cggcaatcca cggcggcact gcttgcagaa aaccgcctcg
    10981 atcagaaaaa gatggatgag ttcgcccagc gcgccgccat gaggaaacct gaatgattcg
    11041 cttagcgccc ttgattaccg ccgacgttga caccaccaca ttgcctggcg gcaaagccag
    11101 cgatgctgca caagattttc tcacgttgtt gagcgaagca ttagcaggcg agacaacaac
    11161 tgacaaagcg gccccccagt tgctggtagc aacagataag cccacgacaa aaggcgagcc
    11221 gctggtcagc gatattcttg ccgatgcgca acaagcggat ttactgatcc ctgtggatga
    11281 aacacttcct gtcatcaacg acgaacaatc cacatcaaca ccgttaacca ccgctcagac
    11341 gatgacgttg gctgcggtgg ctgacaaaaa tacgacaaaa gacgaaaaag cggatgatct
    11401 taatgaagac gtcaccgcaa gtctgagcgc cctttttgcg atgttgccgg gttttgacaa
    11461 tacgcccaaa gtgactgatg caccgtcaac cgtgttaccg gcagagaaac caacgctctt
    11521 cacaaaactg acttctgcgc aactcactac agcacagcct gatgacgccc ccggcacacc
    11581 agctcagcca ttaacaccgc tggtagcaga agcccagagt aaagcggaag tcatcagcac
    11641 gccttcgccg gtgaccgctg acgccagccc gctaatcact ccacaccaga cacagccact
    11701 gcccaccgtc gccgcgcctg ttttgagtgc accgctgggt tctcacgaat ggcaacaatc
    11761 attgagccag catatttcgc tgttcacccg ccaggggcaa caaagtgcag agttgcgtct
    11821 gcacccacag gatttaggtg aagtgcaaat ttccctcaaa gtggatgata accaggcgca
    11881 gatccagatg atttcaccgc atcagcacgt acgcgccgcc ctggaagcag cgctgccggt
    11941 actgcgtacg cagctggccg aaagtggcat tcagttaggg caaagcaaca ttagtggcga
    12001 aagctttagt ggtcagcagc aggccgcttc ccagcaacag caaagccaac gcacagcaaa
    12061 ccatgaacct ctggcggggg aagacgacga tacgcttccg gttcccgtct ctttacaagg
    12121 gcgtgtaaca ggtaacagcg gcgttgatat tttcgcctaa cgtcagaggt agcaccgtaa
    12181 tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg ataattagcc gataagcagt
    12241 agcgacacag gaagaccgca acacatgact gattacgcga taagcaagaa aagcaagcga
    12301 tcgctttgga tcccgattct ggtattcatt accctcgcgg cctgtgccag cgcaggttac
    12361 agctactggc attcgcatca ggttgccgct gacgacaaag cgcagcaacg cgtcgtgccc
    12421 tccccggtct tctatgcgct ggataccttt acggtcaatt tgggcgatgc ggatcgcgtg
    12481 ctttatatcg gcataaccct gcgactgaaa gatgaagcaa cccgctcgcg gctgagtgag
    12541 tatttgccgg aagtccgtag tcgcttgctg ttactgtttt cacgtcagga tgctgccgta
    12601 ctggcgacag aagaaggcaa gaaaaacctg attgccgaga ttaaaaccac actttccacc
    12661 ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg tgctgtatac cgcttttatt
    12721 ctgcgataac gacatgggcg atagtattct ttctcaagct gaaattgatg cgctgttgaa
    12781 tggtgacagc gaagtcaaag acgaaccgac agccagtatt agcggcgaaa gtgacattcg
    12841 tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa cgtttgcagg cgctggaaat
    12901 cattaatgag cgctttgccc gccattttcg tatggggctg ttcaacctgc tgcgccgtag
    12961 cccggacatt accgtcgggg ccatccgcat tcagccgtac catgaatttg cccgcaacct
    13021 gccggtgccg accaacctga acctaatcca cctgaaaccg ctacgcggca cagggctggt
    13081 agtgttctcg ccgagtctgg tgtttatcgc cgtggataac ctgtttggcg gcgatggacg
    13141 cttcccgacc aaagtggaag gtcgcgagtt tacccatact gaacagcgcg tcatcaaccg
    13201 tatgttgaag ctggcgctgg aaggctatag cgacgcctgg aaagcgatta atccgctgga
    13261 agttgagtac gtgcgttcgg aaatgcaggt ggaatttacc aatatcacca cctcgccgaa
    13321 cgacattgtg gttaacacgc cgttccatgt ggagattggc aacctgaccg gcgaatttaa
    13381 tatctgcctg ccattcagca tgatcgagcc gctacgggaa ttgttggtta acccgccgct
    13441 ggaaaactcg cgtaatgaag atcagaactg gcgcgataac ctggtgcgcc aggtgcagca
    13501 ttcacagctg gaactggtgg ccaactttgc cgatatctcg ctacgcctgt cgcagatttt
    13561 aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc gatcgcatca tcgcccatgt
    13621 tgacggcgtc ccggttctga ccagtcagta cggtactctc aacggtcagt atgcgttacg
    13681 gatagaacat ttgattaacc cgattttaaa ttctctgaac gaggaacagc ccaaatgagt
    13741 gacatgaata atccggccga tgacaacaac ggcgcaatgg acgatctgtg ggctgaagcg
    13801 ttgagcgagc aaaaatcaac cagcgaaaaa agcgctgccg acgcggtgtt ccagcaattt
    13861 ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc tgattatgga tattccggtc
    13921 aagctgaccg tcgagctggg ccgtacgcgg atgaccatca aagagctgtt gcgtctgacg
    13981 caagggtcag tcgtggcgct ggacggtctg gcgggcgaac cgctggatat tctgatcaac
    14041 ggttatttaa tcgcccaggg cgaagtggtg gttgttgccg ataaatatgg cgtgcggatc
    14101 accgatatca ttaccccgtc cgagcggatg cgccgcctga gccgttagtg atgaataacc
    14161 atgctactgt gcaatcttcc gcgccggttt ctgctgagcc actgctgcag gtgagcggcg
    14221 cactcatcgc cattattgcc ctgatcctcg ctgctgcctg gctggtaaaa cggctgggat
    14281 ttgcccctaa acgcactggc gttaacggtc tgaaaattag cgccagtgct tcgctgggcg
    14341 cgcgtgaacg ggtcgtggtg gtcgatgtgg aagatgcacg gctggtgctc ggcgttaccg
    14401 cagggcaaat caatctgctg cataaacttc ccccttcagc accaacggaa gagataccgc
    14461 agaccgattt tcagtcggtc atgaaaaatt tgcttaagcg tggcgggaga tcctgatgcg
    14521 tcgtttgttt tctgtcgcac cggtccttct ctggctgatt acgcccctcg ccttcgcgca
    14581 actgccgggt atcaccagcc agccgctacc tggcgggtat caccagccag ccgctacctg
    14641 gcggtggaca aagctggtcg ctcccggtgc agacgctggt gttcatcacc tcgttgacgt
    14701 tcattccagc gattttactg atgatgacca gtttcacccg catcattatt gtttttggtt
    14761 tattgcgtaa cgcgctggga acgccctccg cgccacctaa ccaggtattg ctggggctgg
    14821 cactgttttt gacctttttt attatgtcgc cagtgatcga caaaatttat gtcgatgcgt
    14881 accagccatt cagcgaagag aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc
    14941 cgctgcgtga gtttatgctg cgtcagaccc gcgaggcaga tttagggttg tttgccagac
    15001 tggcgaatac cggcccgttg caggggcctg aagctgtgcc gatgcgcatt ttgctcccgg
    15061 cctacgtgac cagcgagttg aaaaccgcat ttcagatagg cttcacgatt ttcatccctt
    15121 ttttgattat cgacctggtg attgccagcg tgttgatggc attggggatg atgatggttc
    15181 cccccgccac cattgctctg ccctttaaac tgatgctgtt tgtactggtg gatggctggc
    15241 aattgctggt cggttcgctg gcgcagagct tttacagcta gagaggcaaa atgacacctg
    15301 aatcggtcat gatgatggga actgaagcga tgaaagtcgc gctggcactg gcagccccgc
    15361 tgttgctggt agcgctggtc acgggcctta tcatcagtat tttgcaggca gccacgcaga
    15421 ttaacgaaat gacgctgtcg tttattccga aaatcatcgc cgtatttatc gccatcatta
    15481 ttgccggacc gtggatgctc aatctgttgc tggattacgt ccgcaccttg ttcactaacc
    15541 tgccgtatat catcgggtag cccgtacaat gatgcaggag acaagcgatc aatggctttc
    15601 ctggttaagc ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc
    15661 aattctgagc gaacgcagcg tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac
    15721 gttcgccatt gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct
    15781 gtggctggcc gtacagcaga tcctgatcgg cattgcgctt ggttttacca tgcaatttgc
    15841 ctttgccgct gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc
    15901 gacatttgtc gatcctggca gccatctcaa tatgcccgtt ttagcgcgca tcatggatat
    15961 gctggcatta ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt
    16021 cgataccttt cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc
    16081 acccaccaaa gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat
    16141 tactctgttg ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt
    16201 atccattttt gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc
    16261 attaatgccg ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct
    16321 ggctgatatt attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct
    16381 aaggattatc cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata
    16441 tttgttttaa gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta
    16501 tacaacactt tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg
    16561 acccatgttg ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc
    16621 attgagtgag ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta
    16681 ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa
    16741 accgttgatg accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat
    16801 gaggactgtt tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa
    16861 catcccaata cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat
    16921 ctattggtca gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac
    16981 gatatccttg gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg
    17041 acgttatcat tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga
    17101 accattcaaa tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt
    17161 aatattaaac gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg
    17221 acggataatg tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt
    17281 ttcccaccag tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat
    17341 gccctgcttc attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca
    17401 ttgtgccttc actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat
    17461 ccgttttgac tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca
    17521 gtgtagaaca atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga
    17581 cggtctatac ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg
    17641 gttgcgagga aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt
    17701 cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca
    17761 tcgcagccag aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg
    17821 tgaattggcg gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg
    17881 ttgaatgcac aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca
    17941 agaagcactt aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca
    18001 atttttttcg ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag
    18061 aaatgtgacg cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa
    18121 tcatctttga ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac
    18181 catgttttca attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga
    18241 cagtcatagt tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa
    18301 tattcccgtc attctctgta gcagtaaaac tttagcggaa atgctgtact tgcaaaaaat
    18361 gttggggcta caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga
    18421 acaatggcag gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat
    18481 ctgccaggtt ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga
    18541 tgtcgacgat gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac
    18601 gcagctgcat gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca
    18661 atttatcgct cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca
    18721 cgtactggat gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca
    18781 acaattgtca ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc
    18841 tttactggag gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca
    18901 tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg
    18961 gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg
    19021 attgcgcccg gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc
    19081 aaaatcataa tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat
    19141 cgttgtactc ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc
    19201 gacctccgca gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc
    19261 gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga
    19321 aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag
    19381 atcgacctga atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg
    19441 ggctttttcg aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg
    19501 ccactgcaac gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca
    19561 ggagaggagt aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact
    19621 gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa
    19681 atggtccaga cgttcccagc taacatagcg actgtccata cgaatgccgc cccgcgtgtc
    19741 atgttccatt gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg
    19801 atggtcagga ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc
    19861 atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact
    19921 catccccagc acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg
    19981 cggttcagta ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg
    20041 attttctcgt tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt
    20101 tggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac
    20161 atacatcgcc tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac
    20221 ttccagtgca gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc
    20281 ctcgctgact aatgcatccg atacgccatt gactggcagg gtgcgccgtc ggttgagttc
    20341 gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga
    20401 ggtgaaatcc agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt
    20461 gtcgacgtta tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg
    20521 ctagctggag atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga
    20581 aaaaagcaat accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc
    20641 gagttttttc aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc
    20701 caacctgctt ttttatgatt ctggcataac ccggcaatag cgtaccgcaa aacagcgata
    20761 attttataat ggtggcatga taagacgcga ctatcattca ttgcaggcat tgggacacca
    20821 ggccggatgt gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga
    20881 ctcccctccc ccggaacaag atcggtttca tgaaaagctt cccgcttgac cccgtaaccg
    20941 tcataccaac gacactcaac cataccgctg gagtatccag tgacaatcat ccgcgggccg
    21001 ccctctttaa ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga
    21061 gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc ataatagcca gtgcgagtat
    21121 taatgtgcct gaccgcgcat tttcgctact gctttcactc ccagcaccac gatgccgccg
    21181 ataataaatc caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt
    21241 tgctgcccgg cgatatgttc aatagcgtga tgcagcggcg caataccatg taccacaatc
    21301 ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg ataatgcttt catcagccaa
    21361 ggcgcgataa tcaataaccc tttacctaat gcctgcatca gcgcgctaga tttttccgcc
    21421 agccaatacc ctaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg
    21481 gtcactacca gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcctctgcc
    21541 acaatcccca gcgtgatggc gacgatttcc gcagacaaaa taaaatcggt acgaatcgcc
    21601 cctttaattt tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc
    21661 tgctggctct gcgctggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc
    21721 tctacccctt caaagcagag aaacgcgcca ccaatcatca acagtggcgt aatcgcccac
    21781 gggataaacg cactgatgat cagcgccagc ggcaccagaa tcactttatt gatcagcgat
    21841 cctttcgcca cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt
    21901 tgcgcattga gcgataaatc atcccctaat acaccggcgg ttttcttcgc cgccagtttg
    21961 cccatcacag agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt cagtaaacta
    22021 ctcccggcca aaagcattcc ctctcatttt tattgttgaa gggtgtaagt atgaagcaaa
    22081 aacggcagct ctgatacttg cttcacaggt caacatttat ttaacaatca agtgcaatta
    22141 tatctctcgc cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccgccg
    22201 tgagggatta tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat
    22261 atcatttggg gctcaaccta ttttgtcatt cggattggcg tgggaagctg gcctccgtta
    22321 atgatggcgg gcgttcgatt ccaggcagcc ggtattttat tgctggcatt tttgctactg
    22381 cgcggacaca aacttccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta
    22441 ttgctggctg tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc
    22501 atcgccgccg tagtgattgc aaccgtgccc ctctttaccc tgtgcttcag tcgcctgttt
    22561 ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc cggaatcatc
    22621 atgctcaata gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc
    22681 ggctcgatta gctgggcgtt tggctcagtt tatggctcgc gcattacctt acctgtaggg
    22741 atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcatcgatg
    22801 attgcgggtg aaaaactgac ggcgctccct tccctgtcag gcttccttgc ggtcggctat
    22861 ctggcgctgt ttggttcgat tatcgccatt aacgcttata tgtatttaat ccgtaatgtc
    22921 agtccggctc tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt
    22981 acgggactgg gtggcgaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtt
    23041 ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc cggccaaacc cgttgtcgcg
    23101 ccagttattc aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct
    23161 ggcaccttcg ccgcagatcc actcttccag acgctcggta agcgccgcat ccgtcagctt
    23221 ctcgcgccta cgtaacgcac actcccagac aatcagtacg cgccagccga gctcctgcaa
    23281 gcgactgata tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc
    23341 agttcgagtc gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg
    23401 ggtaaatatc acgcagcgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc
    23461 gtcctgaacg cgaaacgcaa ggccctgccc ggttaacaaa ctggcgaggc gcttctcaat
    23521 cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg cgggtggcct tatcgtgaac
    23581 gtcgaccatg ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa
    23641 gcagttttgc caccgcggca aagaccggca cgaccaccga gttgccgaac tggcgatagg
    23701 cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta
    23761 agcgcgcgca ttcacgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt
    23821 caaagtcttt ctcacccgtg gccatatccc aaccgcgatc gattaaaatt tccgcgccat
    23881 ctttgtaata acgagcagac agcgtacggg tgacgctttg cggattgttc ggataaacca
    23941 ttccataacc gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagaggtact
    24001 tccacagcac cggcgtcagg atatatttcg cctcaaccat cgggtccagc agctgcgcca
    24061 gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa tcggctttca
    24121 gattcagatc gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcaggaaat
    24181 gtttaccatc gatgattttc ggatcgtccg gcccgttatc ttctgcatcg gcaacgtcat
    24241 agcccagttc gtccagcgtc tgcatgatga tgcggaacgt ttttccctta tcgtgactct
    24301 tcaggttttt gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca
    24361 ccacatcaaa gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg
    24421 agttcttttt cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa
    24481 catcgtgttc aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt
    24541 cttgatgact gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccgggtcgc
    24601 aataatggtt ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc
    24661 actgtccacc aatcgattca aaaccacgac ggatgccgcc aattccggca aatagatcga
    24721 taaagcgaaa cgcataatgc ggatgatgtt ccggtggttt gggtaacaac gtttgcagat
    24781 gggcaaactc tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta
    24841 aaattgccgc gctccagtga ttctcaccca caccattaag ctgcgccacc agcattttaa
    24901 catcataaat ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg
    24961 aatcggttac tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag
    25021 gttaacataa ttactgccgt ttagggccac gcgctcagcc agcgggtgaa aacgcatcta
    25081 tcaccttgtg atcgatacct tcattctccc ccgccagttc ggcaccgagc ttcgccatca
    25141 aacagcgcca agctcaatgc ccctgaaacg gcaaatacgc gcgccaggcc aatcgccccc
    25201 agggcttcca gccgatccgc atcctggacg attttagctt ccgttgttaa gggggcgatt
    25261 tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg cctcaatttt ctccgccgga
    25321 aattgcacga actcttcacg gagcaggcga cgcgtctctt ctgccgccag gattgaagaa
    25381 cgctgccgtt gcggatgatt tttcgccagg ctcacgatat cgtgaaaata acaggcggtt
    25441 aaaatcacca gcatatcgac atcgtcatct gccgccagtt tctgtgccgt agcccagaca
    25501 cggcgaaaat gacacaggtc atgcgccgcg tcctgatgtt gatgatggtt tttcaaccag
    25561 ttctcgaact atgcctgcca gtgttgtaac tccatacgcg ctccgttgcc aaagagtctc
    25621 aaccttagca gtttttattc atagcgtctg caacgtgcgg gacgaggtcg ggtgtaccat
    25681 gcaatgccgc ctaacaaggc accaaccagg gccataaaca gaaatccgac caatgctcct
    25741 aaccacttcc ccgcggtaga tcctaaatca ctttcaaatc ccgacacggg ggcatcgggg
    25801 atccggctca tcacccagat gtatcgcgcc attgcccaga cgaaatagcc acaccatgta
    25861 tagaaagccc
//
LOCUS       DAJTUM010000055        25821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTUM010000055 DAJTUM010000000
VERSION     DAJTUM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO471_002422"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO471_002422"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6243652.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO471_002423"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO471_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6243653.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO471_002424"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO471_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6243654.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO471_002425"
     CDS             3807..3995
                     /locus_tag="OO471_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6243655.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO471_002426"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO471_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6243656.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO471_002427"
     CDS             4756..5115
                     /locus_tag="OO471_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6243657.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO471_002428"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO471_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO471_002429"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO471_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6243658.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO471_002430"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO471_002430"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6243659.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO471_002431"
     CDS             10913..11254
                     /locus_tag="OO471_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6243660.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO471_002432"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO471_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO471_002433"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO471_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6243661.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13677..14963
                     /gene="dctA"
                     /locus_tag="OO471_002434"
     CDS             13677..14963
                     /gene="dctA"
                     /locus_tag="OO471_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6243662.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15184..16680
                     /locus_tag="OO471_002435"
     CDS             15184..16680
                     /locus_tag="OO471_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6243663.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16776..17705)
                     /gene="kdgK"
                     /locus_tag="OO471_002436"
     CDS             complement(16776..17705)
                     /gene="kdgK"
                     /locus_tag="OO471_002436"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6243664.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17937..18704
                     /gene="pdeH"
                     /locus_tag="OO471_002437"
     CDS             17937..18704
                     /gene="pdeH"
                     /locus_tag="OO471_002437"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6243665.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18774..20834
                     /locus_tag="OO471_002438"
     CDS             18774..20834
                     /locus_tag="OO471_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6243666.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21016..22338)
                     /locus_tag="OO471_002439"
     CDS             complement(21016..22338)
                     /locus_tag="OO471_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6243667.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22749..23762)
                     /gene="yhjD"
                     /locus_tag="OO471_002440"
     CDS             complement(22749..23762)
                     /gene="yhjD"
                     /locus_tag="OO471_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6243668.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23811..24710)
                     /locus_tag="OO471_002441"
     CDS             complement(23811..24710)
                     /locus_tag="OO471_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6243669.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25230..>25821
                     /locus_tag="OO471_002442"
     CDS             25230..>25821
                     /locus_tag="OO471_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6243670.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         6156 a         6484 c         7029 g         6152 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgccaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccataa ccttacaaga cctgtggttt tactaaagga caccctatga
    13681 aaacctctct gtttaaaagc ctttactttc aggtcctgac agcgatagcc attggtattc
    13741 tccttggcca tttctatcct gaaataggcg agcaaatgaa accgcttggc gacggcttcg
    13801 ttaagctcat taagatgatc atcgctcctg tcatcttttg taccgtcgta acgggcattg
    13861 cgggcatgga aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg ctttactttg
    13921 aaattgtcag taccatcgcg ctgattattg gtcttatcat cgttaacgtc gtgcagcctg
    13981 gtgccggaat gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg gtttacgccg
    14041 atcaggcgaa agaccagggc attgtcgcct tcattatgga tgtcatcccg gcgagcgtca
    14101 ttggcgcatt tgccagcggt aacattctgc aggtgctgct gtttgccgta ctgtttggtt
    14161 ttgcgctcca ccgtctgggc agcaaaggcc aactgatttt taacgtgatc gaaagtttct
    14221 cgcaggtcat cttcggcatc atcaatatga tcatgcgtct ggcacctatt ggtgcgctcg
    14281 gggcaatggc gtttaccatc ggtaaatacg gcgtcggcac actggtgcaa ctggggcagc
    14341 tgattatctg tttctacatt acctgtatcc tgtttgtggt gctggtattg ggttcaatcg
    14401 ctaaagcgac tggtttcagt atcttcaaat ttatccgcta catccgtgaa gaactgctga
    14461 ttgtactggg gacttcatct tccgagtcgg cgctgccgcg tatgctcgac aagatggaga
    14521 aactcggctg ccgtaaatcg gtggtggggc tggtcatccc gacaggctac tcgtttaacc
    14581 ttgatggcac atcgatatac ctgacaatgg cggcggtgtt tatcgcccag gccactaaca
    14641 gtcagatgga tatcgtccac caaatcacgc tgttaatcgt gttgctgctt tcttctaaag
    14701 gggcggcagg ggtaacgggt agtggcttta tcgtgctggc ggcgacgctc tctgcggtgg
    14761 gccatttgcc ggtagcgggt ctggcgctga tcctcggtat cgaccgcttt atgtcagaag
    14821 ctcgtgcgct aactaacctg gtcggtaacg gcgtagcgac cattgtcgtt gctaagtggg
    14881 tgaaagaact ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg ccggatggca
    14941 aaacgcacga attatcctct taatctcacc acttatgccc gcactccctt ccccgagtgc
    15001 gggtgtttgc gcataattga cccctgtgcc tgaacttacc acctgggtga gttcaggttt
    15061 atttcacttc ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt gtttttaacg
    15121 tcatttagac tgaccgacaa cgggcagttt ttttattacc aggatagttg atcaggggtt
    15181 cacatgcagg gcataaaaat tcgactttta gcgggcggtt tgctgatgat ggccactgct
    15241 ggctatgtgc aggcagatgc gctccagcct gatccagcat ggcaacaggg gacgctttcc
    15301 aacggtttac agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg tgttgaaatt
    15361 cgcctgctgg ttaataccgg ttcgctcgcc gaaagtacac aacagagcgg ttacagtcac
    15421 gccatccctc gtattgcgct aacgcaaagc ggtggccttg acgcagcaca ggcgcgttca
    15481 ttgtggcagc aggggatcga ccctaaacgc ccgatgccgc cggtaattgt ctcttatgac
    15541 accacgctgt ttaatctgag tttgcccaat aaccgtaacg atttgctgaa agaagcgctc
    15601 tcttatctgg caaatgccac tggcaaattg accatcacac cagaaaccat caaccacgcg
    15661 ctgcaaagtc aggacatggt ggcaacctgg cctgccgata ctaaagaggg ctggtggcgc
    15721 tatcgtctga aagggtcaac cttgttaggt cacgatcctg ccgatccgct gaaacaaccc
    15781 gttgaagcgg aaaaaattaa agatttctat cagaaatggt acaccccgga tgcaatgacg
    15841 ctactggtgg tgggaaacgt ggatgcgcgc tcggttgtcg accaaatcaa caaaacgttt
    15901 ggcgaactga aaggcaaacg tgaaacgcca gctccggtgc cgacgctttc tccgctgcgt
    15961 gcggaagcgg tgagtattat gactgacgcg gtgcgtcagg accggttatc tatcatgtgg
    16021 gatacgccgt ggcagccgat tcgtgaatca gccgcactgc tgcgctactg gcgtgcggac
    16081 ctggcccgcg aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag tgacagcaaa
    16141 gacatcggtc ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg tgccatcaac
    16201 atcgaatcac caaacgacaa gctgaacagc aaccttaatc tggtggcgcg tgaactggcg
    16261 aaggttcgcg ataaaggtct gccggaagaa gagttcaatg cgttagtggc gcaaaagaaa
    16321 ctggagctgc agaaactgtt tgccgcctat gcacgggctg ataccgatat tctgatgggc
    16381 cagcggatgc gttcgttgca aaatcaggtc gtcgatatcg cgccggagca gtatcagaaa
    16441 ctgcggcagg atttccttaa cagcctgact gtggagatgt taaatcagga tctgcgtcag
    16501 cagttgtcga atgatatggc gttaatactg ctgcagccga aaggcgagcc ggaatttaac
    16561 atgaaagcgt tgcaggcggc ctgggatcaa atcatggccc catctaccgc cgctgcgacc
    16621 acctctgtcg ccacggatga cgtacatcct gaagtgacgg atattccacc tgcacagtaa
    16681 tgcctgatgg tgatgagtta gcacaaccat caggtattta tggatgagct ggatagaggg
    16741 gtaacacttt atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc ctcacgcggg
    16801 ataatcgcgc cgcgatactg aataacggta cttgcggtca ggtgcccgcg tttcgccgcg
    16861 ttttccgcac tgccgcccgt caaacgtacc gccagataac cggcgctgaa agagtcgcca
    16921 gctgcggtgg tatcgatcac tttttctttc ggcagtttca ccgccggaac atcaactaac
    16981 gcttcaccag caatggacac caggcaagaa tccgccccgc gtttcaccac cacttctttc
    17041 acgcccgcgt tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc ccacagcgcg
    17101 tcttcgtcgt ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg ctggtacacc
    17161 tgctgtgtct cttctttgct ggcccacagg cgcggacgat agttattgtc gaaaatcact
    17221 tttccgcctt tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg gctggtcggg
    17281 cttaagatcg ccaggctaat cccgctcagg tagagataat cgaaattcgc cagctcttcg
    17341 caaatcgccg cagactgctc gctcgccagc cagaatttgg cggcggcttc gttacgccag
    17401 tagtagaacg tacgttcgcc ggtgctgtcg gtttcaatgt agtaaaggcc cggaagacgg
    17461 ttttccatcc gttgggtcag ggaagtatca acgttctcgc cgtgccaggc gtccagcatc
    17521 tgctggctaa aactgtccgt tcccagcgcc gttacgtaat gaacggttaa tgccgcagga
    17581 tcgacctgac gggcgatata gacggaagtg ttcagggtat cgccgccgaa accgcgctta
    17641 acgtccgcgc ctttctcgga aagctcaatc atgcattcgc caatcacggc aatctttttg
    17701 gacatagtcg tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt ggtggtttgc
    17761 tggtcaacca tattaaaaca gtgttccatt atttttttga gccagaacgt attcctgaaa
    17821 gattccgttg tggagtgagg aaatttaccc cggccaggcg gtaaagttct gcccttacgc
    17881 gccgataatc tttgtcgagt ccgggcagca tcacttttaa acacaggaca tctttgatga
    17941 taaggcaggt tatccagcga ataagcaacc ctgaagcaag catcgagagc ttgcaggaac
    18001 ggcgtttttg gttgcagtgt gagcgtgctt acacctggca gccgatctat caaacatgcg
    18061 ggcggttaat ggccgtggag ctattaacgg tggtcacgca tcccttgaac ccttcgcaac
    18121 gcctgccgcc ggatcgctat tttactgaaa tcaccgtcag ccatcggatg gaggttgtga
    18181 aagagcagat tgatttgctg gcgcaaaaag ccgacttctt tgtagagcac ggcctgctgg
    18241 catcggtcaa tattgatggc cctacgctca tcgccctgcg tcagcaacca aaaatcctgc
    18301 gccagattga gcgtcttccc tggctgcgtt tcgaactggt ggagcatatc cgtctgccga
    18361 aagattcaac ctttgcctcg atgtgtgaat ttggcccgct gtggctggat gattttggta
    18421 ccgggatggc aaatttctct gcactaagtg aagtgcgtta tgactacatc aaaatcgcgc
    18481 gagaactgtt tgtgatgctg cgtcagtcgc cggaaggccg cacactcttt tctcagcttt
    18541 tacatctaat gaatcgctat tgtcgcgggg tgattgtcga gggcgtagaa acaccggaag
    18601 agtggcgtga tgttcagaac tcgcccgcat tcgccgcaca aggctggttt ctttcacgcc
    18661 cggcaccgat ggaaacgctg aatacggcgg ttctggcgct ataagctgcc tcattttccg
    18721 ccgggctgga ctatctttag gactggcaca ggaagaatat gaggaagcga acgatgagca
    18781 aggcaggcaa aataaccgct gcgatttcag gggctttctt gttgttgatt gtcgtggcga
    18841 tcattttgat tgcaactttt gactggaatc gactcaaacc gaccatcaac cagaaagtct
    18901 ctgcggagtt gaatcgtccg ttcgctatcc gtggcaatct gggcgtggtg tgggagcggc
    18961 aaaaacaaga aactggctgg cgcagctggg tgccgtggcc tcatgtacat gcagaagaca
    19021 tcattcttgg taatccaccg gatattcccg aagtcacgat ggtgcatttg ccgcgagtag
    19081 aggcaacgct ggcaccgctg gcgctgctga ccaaaacggt ctggctgccg tggatcaagc
    19141 tcgaaaagcc cgacgcgcgc ctgattcgcc tctctgaaaa gaacaataac tggacgttta
    19201 atcttgccaa cgatgacaac aaagacgcga atgcaaagcc gtcggcatgg tcgtttcggc
    19261 tggataatat tcttttcgat caagggcgga tcgccattga tgacaaagta agcaaagcgg
    19321 atctggaaat ttttgttgat ccgttaggca agccgctgcc attcagcgaa gttactggat
    19381 cgaaaggtaa agcggataaa gaaaaggtgg gcgattacat ttttggcctg aaggcgcagg
    19441 gacgttataa cggtgaaccg ctcacgggta cgggaaaaat aggcggtatg ctggcgctgc
    19501 gtggcgaagg gacgccgttt ccggtacagg ctgatttccg ttcaggtaat acccgtgttg
    19561 cttttgatgg cgtcgtgaat gacccaatga agatgggcgg tgtcgattta cggcttaaat
    19621 tttctggcga ttcactgggt gatctctatg aactgacggg cgttctgctg cccgataccc
    19681 cgccgtttga aacggatggt cggctggtag cgaaaatcga cactgaaaaa tcgtcggtct
    19741 ttgattatcg cggttttaat gggcgaattg gcgatagcga tatccacggt tctctggtct
    19801 acaccaccgg aaagccacga ccaaaactgg aaggtgatgt cgagtcgcgg caattgcggc
    19861 tggcggacct gggaccgttg attggcgttg attccgggaa aggggcagaa aagtcgaaac
    19921 ggtctgaaca gaagaagggc gaaaaaagcg ttcagcctgc gggcaaagtg ctgccttatg
    19981 accgcttcga aaccgataaa tgggacgtta tggatgccga tgttcgcttc aaagggcggc
    20041 gcattgagca tggcagtagc ctgccgatta gcgatctttc tactcatatc atcctcaaaa
    20101 atgctgacct gcgcctgcaa ccgctgaaat ttggcatggc gggcggcagc attgcggcga
    20161 atattcatct ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt caggctcgtc
    20221 gactgaaact gaaagaactg atgcccgatg tggaactgat gcagaagacg ctgggggaaa
    20281 tgaacggtga cgcggaacta cgcggtagcg gtaactcggt ggcggcgctt ttaggcaaca
    20341 gtaacggcaa cctgaaactg ttgatgaatg acgggctggt gagccgcaac ctgatggaga
    20401 ttgttgggct gaatgtcggc aactacattg tcggtgcgat atttggtgac gatgaggtgc
    20461 gggtgaactg cgcggcggcg aatctgaata ttgccaacgg cgtggcgcgc ccgcagattt
    20521 ttgctttcga tactgagaac gcgttgatta atgttaccgg cacggcaagt tttgcttcgg
    20581 aacagctgga tttgactatt gatccggaga gtaaagggat tcggattatc acactgcgtt
    20641 cgccgctgta tgtgcgtggg acgtttaaaa atccgcaggc tggggtgaaa gccggaccgc
    20701 tgattgcccg tggtgctgtt gctgcggcac tggcaacgct ggtaacaccg gcggcggcgt
    20761 tactggcact gatctcacct tccgaagggg aggctaatca gtgtcggacg atattgtcgc
    20821 agatgaagaa gtgatgataa ttgccggatg tctgacatcc ggcaattaaa cagatttaga
    20881 tttttggcaa agtgtgcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    20941 aaatccttgt gaattcaata tattgtatgg aaattcagtc ctgataagcg tagcgcatca
    21001 ggcttttcac acttattaca acgactgatg tcgcgtctca tgggtcagca gcagggcgat
    21061 taacgtcaag ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagc
    21121 ctgcaaccag gctgcgatat acggcgcaac ggaagccccg agaatcgacg atacgttgta
    21181 agagaacgat gctccggtgt aacgcacttc tgtcggaaac agctctggta acagcgcacc
    21241 catcggcccg aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag
    21301 aatcgggttg ccagaaccga gcagcgggtt aaaggcgaac agcgcgaaca ggatgatcag
    21361 cgtggtgatg atcaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac
    21421 tggcaccatc acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg
    21481 cggcaggcca agcccaactg gcgcggcggc ggtactgaag gtcatagagt agaccgtcat
    21541 gatgtaaaac agcgtatagg ttgccagcat aatgaacgta cccagtacgg ttacgcgaac
    21601 atgtttggtc agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt
    21661 ctcaaacacc ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagcac
    21721 cgccgagaag ataaatggca cacgccagcc ccagctcata aactgctcat cggtcagtag
    21781 ccaggaaagc agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcctagctg
    21841 tgggaaggag ccatacagtg cacgtttgcg cggtggggcg ttttcagtcg ccagcagcgc
    21901 cgcgccgccc cattcaccgc ctaagcccag accctgacca aatcgagcca gcgccagcag
    21961 cagtggggcg aaaataccaa tcgtggcata gcccggcagc agaccaatca ccacggtcga
    22021 aatccccatc gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa
    22081 atgaccaaaa acggcagagc caatggggcg cgcgacgaag gcgatggcga aggtggcgag
    22141 cgactgtagc gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg gaaacacaat
    22201 aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc caatgagaga
    22261 ggcgacaagg actttattac gcgagttgat cggcgtgtat tcttgttcgt ggtcgagtgt
    22321 tgtggctgtt gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc
    22381 acagcaaaag tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa accaggcaaa
    22441 aaggggataa tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata
    22501 aatagtggat aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg
    22561 ggttatgagt tggagtgaaa ttgtttgctc ccaaatagat ggctgaagag gctctgggga
    22621 agagtttcaa gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg
    22681 gcctacgggg agccaggccg aataaaatcg tatgccggat aaggcgttta cgccgcatcc
    22741 ggcctatttt aaggctgcgt tttccctggc attcgcgggt cgtctttata ttcggcggtg
    22801 gcaatccacg ccgcgcaaaa cagcgtcaaa cgggcgaaga agtagaaaaa tgccatcagc
    22861 cccagcacgg aaccaaatgc tgcgccagag ggggatttca tcagcgatgg cagggtgtag
    22921 gtcatcacga ttttaatcac ttcaaaacca atagcagcca gaaatgttcc gcgaatcagg
    22981 gcttttttgc gcggacggtg gcgcggcaac cgccagaaga tccagaagaa aagcagatag
    23041 ttggcgaaga tggaaatcgc caggccaatc aatcgccacg tcggtttcag ccactcaatg
    23101 ctgttcaggt gcagggcgct aataatcatt tgctgcgccg aaccggcaac cgaggtgatc
    23161 gaaagcgtca caatcagcgc aatcaacaaa ccaatcagtg aaataaaatc acgcagatat
    23221 tttacccaga acttctcctg atcttgcggc gagcgttccc agacatcgcg cgactgggca
    23281 cgaatcgctt cacgcaggtt acccatccag ttgatgccgg aataaagtgc caccgccagg
    23341 ccgacaagcc ctacagtcgt acgctgctga acggcggtgt tgatggtgtt tttcaacgtg
    23401 gcggctagtg tcggatcgct gatgttttgc agaattttgt cgaagatatc ctgtagcaac
    23461 atcggatggg aggccagcac aaagccccct gcggcaaacg acaccatcaa aatcgggatc
    23521 atcgacaaaa atgagaaata ggtgatagcc gcaccaaact ggttacccag ccgatcgtta
    23581 aagcgttctg tcgcgcgaat aaggtgcgca ataaccggtt ggcgttggac tttttcggca
    23641 gtggtggtga cggtttccag cgcctggcta actttgccgc ctttctcgct attatccaac
    23701 tgcttaatcg gctcgtgctc cagatcctgg gtgggacgtt tgatctcgtt ttcctgcgtc
    23761 atccgctctt ttgtccttct gtcgtggtca ttcttaaaaa gtatagtcgg tcagtccagg
    23821 tgctctttca ttacgccagc cagccactgc ataaacaggt ttacacgccg ggaaagctcc
    23881 cgacgctgcg ggtaaaccag cgaaagggag agcggctcgg cacggtagcc tggcaacact
    23941 tcaataagcc gcccggcacg cagggcttcg cgcacggcaa tgcgcgggat ctgaataatc
    24001 cccagaccgg caagaccggc ggcgagatag ttttcgctac tgtttaccgt caacatgccg
    24061 ccagacttaa accactggac gccattaacg ctggcaacct caaaacctaa cggatgtaca
    24121 cccaggtgcg gtgtataacg cactattgcg tgtgaagtca gatcatcggg gctttgcgga
    24181 taaccaaagc gcgtcagata gtgcggactg gcacagttga ccatggttag tttgccgagg
    24241 ggacgggcga taacgccatc ttccggtaac gcgccagtgc gtatcacgca atcaaaacca
    24301 tcatgaagaa tatctaccgg acggttatgg ctactcagtt ccagctcaat tcccggatgc
    24361 tgatagagaa attccgacag acgcggcagt aacaggtttt tcgcgattcc tgacgggatg
    24421 tcgatgcgta atttaccgct gatactggtg gcatcctgtt gaaacagacc gtccagttcg
    24481 ctgagattac tcaacacatc ttttgcccgt tgataatagg tcatgccttc tggcgtgagt
    24541 ttgacccgtc gcgtagtgcg ctggagaagc tgggtgccaa gttgatgttc cagtgcctgt
    24601 atctggcgcg aaacacttcc ctttggcaaa gcaaagaaat ccgccgcgcg ggaaaaactt
    24661 tccagctccg cgactttgat gaacaactgc attgcgtgaa ttttatccat ttctttgacc
    24721 gattgttgtt cacagcgaaa cagtaacgcg catatcgcat tgtttattgt tttttctaac
    24781 atctaatgcc gggtttcaca atgttgtcat gacgaatgtg gcgatttctc agcaggaaaa
    24841 gggcggaata gctttatgag aaaggtgaat ccccttaaac agagcgtatt tttcagtgat
    24901 tccctttaaa gtgaatagag gacgtattgg ctatttgtgc ttatttttgt ctcattgcca
    24961 tttgttattt tggcttgtag tcgcggaatt tcatttttaa ttgcatgcgt actaaccacc
    25021 aatataagga aatcctcaaa attgttttaa atagtgccct cgacatgtaa tttcaggcgt
    25081 ttagctgtaa ctatttatat cggtaactat tttctacgcg tcatctctgt agaatggcca
    25141 ccgccaaatg gcgaattctt attttttctc gcgggtaata cccttccact acttaagtct
    25201 ttttaacgta ttcaggtgga gattcagtta tgcaaatagt catgtttgac aggcagtcaa
    25261 tatttattca tggaataaaa atcagtttac agcagcgtat tccaggagtg agtattcagg
    25321 gggtcagtca ggcagacgag ttatggcaaa agctggaaag ttaccctgaa gccttagtta
    25381 tgctcgatgg cgatcaggat ggtgagtttt gctactggtt gctgcaaaaa accgtggtgc
    25441 agtttcctga ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg
    25501 aagttatcca ttttaatgtg ctggccattg tgcctcgcga ttcaaccgtc gagacatttg
    25561 cgttggcggt aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga
    25621 cgccggaaaa ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa
    25681 ccatgttagc ggcgggcgaa tcaaataagg agatcggcag agcgctgaat atcagtaccg
    25741 gaacggtaaa agcgcatctg gaatccctgt atcgccgtct ggaagtgaaa aatcgcaccc
    25801 aggcggcaat gatgttaaat a
//
LOCUS       DAJTUM010000056        25661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTUM010000056 DAJTUM010000000
VERSION     DAJTUM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="OO471_002443"
     CDS             complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="OO471_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6243671.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQ"
     gene            1796..2542
                     /gene="mipA"
                     /locus_tag="OO471_002444"
     CDS             1796..2542
                     /gene="mipA"
                     /locus_tag="OO471_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6243672.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2632..3393
                     /locus_tag="OO471_002445"
     CDS             2632..3393
                     /locus_tag="OO471_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6243673.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3444..4328)
                     /locus_tag="OO471_002446"
     CDS             complement(3444..4328)
                     /locus_tag="OO471_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6243674.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="OO471_002447"
     CDS             complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="OO471_002447"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6243675.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5749..6162
                     /gene="msrB"
                     /locus_tag="OO471_002448"
     CDS             5749..6162
                     /gene="msrB"
                     /locus_tag="OO471_002448"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6243676.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6204..6476
                     /locus_tag="OO471_002449"
     CDS             6204..6476
                     /locus_tag="OO471_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6243677.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6846..7922
                     /locus_tag="OO471_002450"
     CDS             6846..7922
                     /locus_tag="OO471_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6243678.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7949..9328
                     /locus_tag="OO471_002451"
     CDS             7949..9328
                     /locus_tag="OO471_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243679.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9345..10390
                     /locus_tag="OO471_002452"
                     /pseudo
     CDS             9345..10390
                     /locus_tag="OO471_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10411..11247
                     /locus_tag="OO471_002453"
     CDS             10411..11247
                     /locus_tag="OO471_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6243680.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11252..12199
                     /locus_tag="OO471_002454"
     CDS             11252..12199
                     /locus_tag="OO471_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6243681.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12209..13189
                     /gene="ydjG"
                     /locus_tag="OO471_002455"
     CDS             12209..13189
                     /gene="ydjG"
                     /locus_tag="OO471_002455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6243682.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13326..14084
                     /locus_tag="OO471_002456"
     CDS             13326..14084
                     /locus_tag="OO471_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6243683.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14202..15560
                     /locus_tag="OO471_002457"
     CDS             14202..15560
                     /locus_tag="OO471_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243684.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="OO471_002458"
     CDS             complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="OO471_002458"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6243685.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="OO471_002459"
     CDS             complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="OO471_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6243686.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="OO471_002460"
     CDS             complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="OO471_002460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6243687.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19505..20056
                     /locus_tag="OO471_002461"
     CDS             19505..20056
                     /locus_tag="OO471_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6243688.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20173..21216
                     /gene="selD"
                     /locus_tag="OO471_002462"
     CDS             20173..21216
                     /gene="selD"
                     /locus_tag="OO471_002462"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6243689.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21221..23182
                     /gene="topB"
                     /locus_tag="OO471_002463"
     CDS             21221..23182
                     /gene="topB"
                     /locus_tag="OO471_002463"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6243690.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHTPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23309..24349
                     /locus_tag="OO471_002464"
     CDS             23309..24349
                     /locus_tag="OO471_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6243691.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24466..>25661)
                     /gene="gdhA"
                     /locus_tag="OO471_002465"
     CDS             complement(24466..>25661)
                     /gene="gdhA"
                     /locus_tag="OO471_002465"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6243692.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5972 c         6624 g         6548 t
ORIGIN      
        1 tctgctgttc caggacgtag aacagatgga ccgggtttgc cgccacttct acatgatcga
       61 agttgaacac gcgggagagg atcttaaacg caaaacgcgt cgacagaccg ttcatccctt
      121 catcgacacc ggcgtagtca cgatattcct gatacgactt ggctttcgga tcggtgtctt
      181 tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt
      241 tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag cgtgccaggg gcgcatgggg
      301 cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc
      361 gcaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt
      421 tattacggaa agtgacccat tcggacttgt tcgagtgggc gagaataatc ccgttgaacg
      481 gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg gttaacaagg
      541 gatgcagcac ttttatcggt gctttaaaca tctcaacgaa ttccatgatc ccctgattgg
      601 cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg tagtgttcga
      661 gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg
      721 gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga
      781 acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc gacatgatgg
      841 tgccgagata acggcgagga atgccatact ctttttccag aatctgagca tcttcctgcg
      901 gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata
      961 cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc
     1021 cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt
     1081 gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg
     1141 ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggg tcctgggctg
     1201 tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag
     1261 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg
     1321 cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt
     1381 tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg
     1441 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa
     1501 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
     1561 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
     1621 taaagaccag gctcgccagt aacgctaaat ccatttggct gtaagcgcgg tgtcatccgc
     1681 ggcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg
     1741 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
     1801 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
     1861 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta
     1921 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
     1981 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt caattaccgc
     2041 ttactggtcg ccgctttact tcaaagcgaa agacagtggc gatcaccaaa tgcgtcacct
     2101 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
     2161 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca ttgtctggga
     2221 tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt
     2281 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     2341 tcgcagcggt ctgcgtggct ataacccgaa tgacagctgg agcccttacc ttgagctgag
     2401 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct
     2461 gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc tgatttctac
     2521 cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaacag
     2581 ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa
     2641 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     2701 atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc aggcattgaa
     2761 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg
     2821 gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa agtctatccg
     2881 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     2941 actgactatc tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact
     3001 gttgctgcga tggaaaaatt gatcgcccag ggaaaaatcc gtcgctgggg cgtttctaac
     3061 cttggttatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     3121 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc
     3181 cagcaacagc agatgccggt gatggcttac agtccattag cccaggccgg gcggttgcgc
     3241 aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgtccaaca acatgcagct
     3301 gcgcttgagg tcgaactttc gtcagcggaa ttagctatgc tggataaggc atatccggca
     3361 ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac
     3421 ccggcacaca acattaacgc aaattaacgt ttggcaacgc gaatggattg cgccagatgt
     3481 gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca
     3541 aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg
     3601 ttccagccga caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct
     3661 tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg
     3721 aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag
     3781 cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc
     3841 agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc
     3901 acgcggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt ctcttcgctt
     3961 tgtgtcaatt caaaagtcag cgctacgcca tcagcatctt catgatgtga tttcagcgtc
     4021 cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac
     4081 cacggccagc aaaccggtac gccaccgcga atagcgacgc catttttgaa cggtgtgttg
     4141 ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc
     4201 tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc cagttcatcc
     4261 agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc
     4321 ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt
     4381 cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg atcaggtcca
     4441 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
     4501 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     4561 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     4621 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     4681 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     4741 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     4801 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     4861 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     4921 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     4981 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     5041 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     5101 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac acccacttcg tcccatttca
     5161 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     5221 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     5281 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     5341 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     5401 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     5461 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     5521 aagcattacg cgactgactc gcctcacttt tcctttgggc ttgagaccac atggggtcgg
     5581 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     5641 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     5701 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagtaaat ggcgaataaa
     5761 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     5821 catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     5881 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     5941 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     6001 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     6061 gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc tgcttcttta
     6121 cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     6181 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     6241 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat ggcgttgcgt
     6301 tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa gcccgccata
     6361 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     6421 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
     6481 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcgtc
     6541 tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg
     6601 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
     6661 tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
     6721 aattgtgatt tgagttgtaa aatgcaggtc attatctgtg ttgtgcagat ttaatattga
     6781 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
     6841 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattcatg
     6901 tcccacaacc catttgtggc ccggaagacg tagtgactga aattaaagcc gcggcaatct
     6961 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
     7021 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
     7081 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg
     7141 aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
     7201 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
     7261 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
     7321 tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgttg
     7381 tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg attatggggg
     7441 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     7501 aagaactggg ggcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
     7561 agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt gccaatatcg
     7621 cactgaaaca agccatcgaa atgctccgcc cgaatgggga agtggtacgc gttggaatgg
     7681 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa agcatcattg
     7741 ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta gccagcggcg
     7801 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
     7861 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
     7921 aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgc
     7981 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
     8041 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
     8101 gttgggctgg acagataatt ccactacagc cacattctca gcaatcacga ccgccggaat
     8161 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
     8221 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
     8281 ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt tactggttac
     8341 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
     8401 ggtttccttt attggcaact ggtcatatcc gctctgctca ctgattgcga tgggactcac
     8461 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg caatattgtc
     8521 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
     8581 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
     8641 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
     8701 ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
     8761 tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
     8821 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
     8881 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
     8941 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
     9001 tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata tgtacgtttg
     9061 ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac tccgtggctc
     9121 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
     9181 gctgctcagt agttatggtg taacgggagt ctttattctt ctgggggctg tttcaaatat
     9241 tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg ttgaaagttt
     9301 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
     9361 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca gtgcctaaag
     9421 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
     9481 ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
     9541 aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc
     9601 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
     9661 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
     9721 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
     9781 tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
     9841 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
     9901 aagcgtgcaa atgcctgggc gcaacggaaa ttgccgtcgt tgatgtactg gaaaaacgtc
     9961 tggcaatagg ggaacagcct ggtgcgacag tggttattaa tggcgcaaaa gaggacacta
    10021 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    10081 gttctgcggt caccgttaaa caggcacctt taatctggta atgcgcggcg gtaaaattat
    10141 gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca atcgcgaagt
    10201 cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg aagctatttc
    10261 ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc gggatgtaca
    10321 acaggcattt gaagagtcag ttaacaacaa acgcgacatt attaaaggcg ttattaaaat
    10381 tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata ttgggaaaac
    10441 gatgccacaa ataaatatta tgcaattgcc cattttaacg tatggaatgc agaaatgttg
    10501 atgggcgtta aagacgcagc cgaagaagcg aaatccccgg ttattatttc ttttagtaca
    10561 ggttttgtcg gtaacacctc atttgaagat ttctctcaca tgatggtatc aatggcacaa
    10621 aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtattga gattattcat
    10681 aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt tgatttcgaa
    10741 gaaaatattc gtttaaccaa agaggctgtt gatttctttc atccgctggg tattccggta
    10801 gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct ggcgggctat
    10861 cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg tgactcattg
    10921 gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccgcaatt gaactttgag
    10981 gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg cgcatcgggg
    11041 atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat caacattcat
    11101 acggagctct gtcaggccgc aatggtagca gtgaaagaaa accaggatca gcctttcctg
    11161 catttagaac gtgaagtacg taaagctgta aaagaacggg cattggaaaa aattgaactg
    11221 ttcggttctg atggcaaagc ggaataatga tatggataat ctcgacgtta tttgtatagg
    11281 tgccgctatt gttgatattc cattacaacc ggtcagtaaa aatatctttg atgtagattc
    11341 ttatcctctt gaaagaatcg caatgagcac cgggggcgat gcaataaatg aagcaacaat
    11401 tatttctcgt ctggggcatc gtaccgcatt aatgagccgc attggtaaag atgcctcagg
    11461 gcaatttatt ctcgaccatt gtcgcaaaga gaatattgat attcaaagcc tgaaacagga
    11521 cgtcagcata gatacatcta ttaacgttgg actggtgacg gaggatggcg agcggacgtt
    11581 tgtcacgaac cgtaacggca gtttgtggaa attaaatatt gacgatgttg attttgcgcg
    11641 gtttcctcag gcgaaattat tatcactggc cagtattttc aacagtcctc tattggatgg
    11701 taaagcgcta acagaaattt ttacgcaagc gaaagcccgg cagatgatca tttgtgccga
    11761 tatgatcaaa ccgcggttga atgaaacgct ggatgatatt tgcgaagcat tgagttatgt
    11821 cgattatctg tttccaaatt ttgccgagga aaaattactc acagggaaag agacactgga
    11881 tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa
    11941 agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat
    12001 aaccgccatc gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact
    12061 gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc
    12121 ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct
    12181 ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca cgacggatat
    12241 aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg
    12301 cgatctcgat cggcaaatat gtattgatac gattcttgaa gcccatcgtt gtggcattaa
    12361 tctgattgat actgcgctag gatataactt tggcaatagt gaagttatcg tcggtcaggc
    12421 gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga
    12481 acgaaaagga agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc
    12541 ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg attacatcga
    12601 tatctatatg acgcactggc agtcggtgcc gccatttttt acgccgatag ctgaaactgt
    12661 cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt
    12721 cgatgctgac catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa
    12781 atacagtatt ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg
    12841 cattgtggtt caggtttatt ccccgctaga gcagggattg ttgaccggca ctatcactcg
    12901 tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct
    12961 gaaagtgatt gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat
    13021 tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg
    13081 ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga
    13141 tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa tactcaacac
    13201 gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat aggttatttt
    13261 gaaagtaaac atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg
    13321 taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa
    13381 aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg
    13441 atcttgagaa gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa
    13501 atacagcggt gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag
    13561 agaagctgct tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag
    13621 ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga
    13681 cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg
    13741 tttcaacggg tggggaacta aacaaaaata cactttccct gcaaggaaga attactaaag
    13801 agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta
    13861 acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc
    13921 aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc
    13981 agttagctga ctttagtcat attaattata taataactga taaatcaccg ggtgcagagt
    14041 ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacacgttgt
    14101 caatattatt tgttcctgcg actggaaata aaatgatgat aaagatgcgg caagtgaatt
    14161 tgccgcaatt gctacctttt atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa
    14221 ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtataa
    14281 taagctttag tctgttatta acggggtttt tgagttactc aggtaatgtc gtcttagcaa
    14341 agctggtaag caatggatgg tcaaataatt tcctctatgc cgcctttacc tcggcattaa
    14401 tgtttggtta tttcatcggc tcacttactg gtgggtttat tggtgactac tttgggcggc
    14461 gcagggcgtt tcgcataaat cttctcatcg tcggtattgc tgcaacaggg gccgcttttg
    14521 tccctgatat gtactggctc attttctttc gcttcctgat gggaacagga atgggggcgc
    14581 tgattatggt tggctatgcc tcatttacgg agtttatccc cgcgacggtg cgtggaaaat
    14641 ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctgtctgcg gcgataggcg
    14701 tggtggttat cgcttttttt agttggcgaa taatgtttct tctgggtggt attggcatac
    14761 tgttagcctg gtttctctca ggtaaatact ttatcgagtc gccacgatgg ctggaaggga
    14821 aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg
    14881 agaagagtat tcgtttaccc cagcttactt tgaaccagag caacagcaag gttaaggtaa
    14941 tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga
    15001 taactgtttt aattgcaatg aacatttcgc tttataccat taccgtatgg ataccgacca
    15061 tatttgttaa ctccggcatt gatgtcgata aatcaatatt aatgaccgct gttattatga
    15121 ttggtgctcc ggtaggaata tttattgcgg cattaattat tgatcatttt cctcgtcgat
    15181 tatttggctc cgccttactt attattattg ccgtgttagg ctatatctat tcaagtcaga
    15241 ctacagagtg ggcgatttta atctatggtc tggtgatgat cttcttttta tacatgtatg
    15301 tttgcttcgc gtcggcggtt tatatcccgg agctttggcc aacgcattta cgcctgcgcg
    15361 gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg
    15421 cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt gtcatgttag
    15481 tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg tcgctggaag
    15541 agatttctga ggtgaattaa atctttcgtt gatggcatca attgccagat gtaccactga
    15601 cgtgttttat ttggcctaca atgccagtac agaattgtag gccagcgtaa aattacccct
    15661 gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac atctccataa
    15721 acgcgtgtgc actgtcctgg ggctggatat tcacgccacg acagccatcg gtaatcacgt
    15781 ttaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag
    15841 ccaggcccat aacgatcaat tcatcgattt catgatcgcg caaccagtca tcaagcgcgg
    15901 ttttctgccg acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc
    15961 ctttatggaa caccgctgcg atctcttttt gattcagtaa cggatgtaat tgtgcgcctt
    16021 cactgttctg cacacagtgt tcaggccaga aagtttgccg caggccgccg agttggcctg
    16081 gcgtataagg ctctacgccg tgctggctgg caaagctgcc gtgattcgcc ggatgccagt
    16141 cctggctggc gataaccgct tctccgcgcg actggcacca gtcaataagg cggttagcaa
    16201 cctccaccgt actgtcacct tccggtacgg cgagcgcgcc accagcacag aaatcatttt
    16261 gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt ctccttaatc atccggcgtc
    16321 agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg
    16381 ctcagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgtcaata
    16441 acgccggcat gggcgagggc gttaccggtg gcataaccac tcatattcac tttaccggac
    16501 atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc
    16561 aggaaggctt tgttttgtgg cgcattaccc acgccatagg agcgcagaat caatgctttc
    16621 accggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccctggata aatcgtcacc
    16681 acgccaattg gttgtggcgt gattggatga acgattaatt ccccttcacc gtgcggggcg
    16741 ggtggcgtat tcaaacgacg aatatggata ccagcttcca gtaacggagg aaggtttgga
    16801 gaggcaaacg catcaaaacc atcggcatgg gctttggtag tgcggttgcc gcgatacaat
    16861 cggttattga aaaagagcgt tacttcgttg atcggataat tcggcgcaac gtacaacgca
    16921 ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc
    16981 acaatgaccg gtttaccgag attctcgagc atgaacgaca gcgcagaggc ggtatacgcc
    17041 atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata
    17101 tcttcagcaa tatgctgcca gtcttccggc gtcatatcag aagaatccat caacggcgta
    17161 tattcatgaa tggtgaaatc tggcatctcc gggcgatgga attccggcat cagcgccagt
    17221 tggcgttgta gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg
    17281 gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata
    17341 tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc acctgatact atgcggataa
    17401 ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaata atgtggccga
    17461 cgatctgtcg gccactcagt acaagactta acgcacgttg gcgcaggtca ggcaaaacgc
    17521 ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga
    17581 ggctaccgag gcaagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg
    17641 gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta
    17701 ttccagatgc cactgtttca cttttgcgag ctctgctgct ttggcaaccg catcatcgaa
    17761 atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc
    17821 ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat
    17881 aaagcgttta tagccattct caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag
    17941 tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc
    18001 aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt
    18061 cagggtgctg gggttagcca caatgtaatt agctggcgtg gaaatccagt aaccaccaga
    18121 tgccgccatg ccgcccatcg atacaaccac agacttaccc gctgcccggg ctgctgccag
    18181 ttcagcgcga atcacttcag acgcggtaac gctgccgcct gggctattaa cacgcaggac
    18241 aatcgctttc actttcgggt caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc
    18301 gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc
    18361 gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg
    18421 ataattttta tcagtcttac tccagccgaa ctctttggtc agagcttttt cgatttcggc
    18481 actcgatgcc agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc
    18541 ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat
    18601 ctgccggtta gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct
    18661 gtcagcttcg cgggctgccg gtgacatatc atcacgaata aacggttcaa cggcagattt
    18721 atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagca acgatttgta
    18781 gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg acagccaaat
    18841 tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc caacggcata
    18901 aaccggtttc ccgctgtcac gaaactcttt cagggctttg ccgatgtact gcatagacgg
    18961 ctggtcgccg cctgcgaagt ttttcagatc catcacgata ccggtgatat tgcggtcgtc
    19021 cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca gacgatcgga
    19081 actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt ctggtttatc
    19141 gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc
    19201 accaccactg acctgcatcc agatccccac accaaccagt acgaggaaaa taaagaacag
    19261 gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc
    19321 aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc tgtcacaggc
    19381 gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg
    19441 tgctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcacga caggagttaa
    19501 tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc tggctgaacc
    19561 cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca
    19621 taagtccatg caaccgtggc atttttttgt gattgaaggg gaagggtgcg agcgtttcag
    19681 cgccgtactg gaacaggggg cgattgctgc cggtagtgat gataaagcta tcgacaaagc
    19741 ccgtaatgcg ccgttccgcg caccgctcat catcacggtg gtggcgaaat gcaaagaaaa
    19801 tcataaagtc ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca tggcgatgca
    19861 aatggcagcc gttgcccagg gatttggcgg catctggcgc agtggcgcat taactgaaag
    19921 tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta
    19981 cctcggcacg ccgcagttga aagcatctac gtcgattaac gtcccggacc cgacgccgtt
    20041 tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa
    20101 ttcagacact ctcacttatc acttcacgga atgagggtta ccatacccgg attgcattga
    20161 caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggagctggt
    20221 tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag
    20281 tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat
    20341 ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat
    20401 cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt cgcgatgggg
    20461 ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa
    20521 attgcccgcg aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg
    20581 gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc
    20641 gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg
    20701 acgaaaccgc tggggatcgg cgttcttacc acggctgaga aaaaatcact gttgaaacca
    20761 gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg cgcgtccttt
    20821 gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gctttggtct gctgggccac
    20881 ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg tcgactatga agcgatcccg
    20941 aaactccccg gtgttgaaga gtacattaag ttgggcgcag tacctggcgg cactgaacgt
    21001 aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg
    21061 tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag
    21121 gttaaaacta cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca
    21181 gcgcgcggcg gtcgtgccat ggttgagatc cgttaattta atgcggttgt ttattgccga
    21241 aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga
    21301 tggctttatc gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc acctgcttga
    21361 gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc
    21421 gattgtcccg gaaaagtggc aattacagcc acgaccctcc gtgaccaaac aacttaacgt
    21481 catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggggacc cggatcgtga
    21541 agggcaattg ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca
    21601 gcaggtgcag cgttgcttga taaacgacct gaacccgcag gcggttgagc gggcgatcga
    21661 ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg
    21721 tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc
    21781 cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt
    21841 gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca
    21901 tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga
    21961 accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa
    22021 ccgcattagt ggtcaaccgg ctattgtcac cagctataac gataaacggg aatcagaatc
    22081 cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct
    22141 gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca agctaatcac
    22201 ttatccgcgt tccgattgtc gctatttgcc agaagaacat tttgccggac gccacgcggt
    22261 gatgaatgcc atcagcgttc atacaccgga tctgttgccg cagccagtgg tagatccaga
    22321 tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac
    22381 cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc
    22441 ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact
    22501 ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg
    22561 cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccat tgcctgtggt
    22621 ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc
    22681 gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt
    22741 gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga cagaagcaac
    22801 ccgtgccggg attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta
    22861 tatccactcc accgacgccg gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg
    22921 accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg
    22981 ctatcaggac tttatgcagc cgctggtggg gacgctatat cagcttattg atcaagccaa
    23041 acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga
    23101 taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc
    23161 cggaagcggg gcgatagcgt aagcgagcga atctttcgtg ctattcgagt catattctga
    23221 aatatccagc ggatcaagaa agttcgttgg atattttttt gcatggataa aattatcgcc
    23281 tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt ttattgcaaa accatcctgg
    23341 gagcgagaag tattctttta atggctgggg aatatttaat agtaattttg aacgggtgat
    23401 taaagaaaat aaggccatgc tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc
    23461 tgtaatgttt gtattcgcag cgataacatc caacggtttg aatgaaagag gcctgattac
    23521 cgcgggatgc tcttttcttt atctattaat tatgatgggg cttattgttc gggccggttt
    23581 taaagcaaaa aaagaacaac tgcattatta tcaggctaaa ggtattgagc cgctcagtat
    23641 cgagaagtta caggcgctac aattgatcgc accttatcga ttctatcata agcaatggtc
    23701 tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca
    23761 tgtgcttcct tttgactcga tcgatatcat cagtaaaaga cgcgagtctt tagagaatca
    23821 atggggtatc gaagatagcg aaagttattg tgccttaatg gagcattttc tttctggcga
    23881 ccatggagcc aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt
    23941 gttgaataaa tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc
    24001 cggtaaatcc tcggcgaagc taatatgggc ggcggaatta tcatggatga tctcgatatc
    24061 aagcacagct tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct
    24121 tgcttctcga aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct
    24181 aatgggattt ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact
    24241 tgaagcgtgt tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg
    24301 gcccataaga aatgttccct ggggagcatc atccgttaaa tactcataaa cgcctgaaat
    24361 tttgccgggg gcgctgcgct tgaccggcct acattagagc gtaactgcaa tgaattgtgc
    24421 ccatctgtag gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg
    24481 cgccagcatc gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt
    24541 ggtttgctca ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg
    24601 atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat
    24661 ttccaggccc gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac
    24721 gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcagc
    24781 gacggcttta acgccattgg cgataagctg atgcgcggcg tcaacatcca gttcgttctg
    24841 ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata
    24901 gaccagacca aattctttgg cgtaatctgc cgctcgacca tcgcggctgg ctttgatttc
    24961 gataagacgt gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga
    25021 gtctgacgca gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc
    25081 gacgttgccg gagccagaaa cggaaacgct catcccttca aaacccatac cgtggcgttt
    25141 taacattgct tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact
    25201 accgccaaat gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt
    25261 catcatcccc gccataaagc cgacttcacg accaccaacg ccgatatcac ctgccggaac
    25321 gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc agcgtctggc aaaaacgcat
    25381 cactttacct tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat
    25441 cggcagggta gtcagggcat ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga
    25501 aaggttaact gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa
    25561 ctgcacacgc catgcacggt tgacctgtat ctggttgcga tcatcaaccc ataccacgcg
    25621 aaactggatc acgcgctccg gttcaaccag acgctccagt a
//
LOCUS       DAJTUM010000057        25095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTUM010000057 DAJTUM010000000
VERSION     DAJTUM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..887)
                     /gene="ycjM"
                     /locus_tag="OO471_002466"
     CDS             complement(<1..887)
                     /gene="ycjM"
                     /locus_tag="OO471_002466"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6243693.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQ"
     gene            complement(1099..1446)
                     /gene="pspE"
                     /locus_tag="OO471_002467"
     CDS             complement(1099..1446)
                     /gene="pspE"
                     /locus_tag="OO471_002467"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6243694.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1489..1710)
                     /gene="pspD"
                     /locus_tag="OO471_002468"
     CDS             complement(1489..1710)
                     /gene="pspD"
                     /locus_tag="OO471_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6243695.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1719..2078)
                     /gene="pspC"
                     /locus_tag="OO471_002469"
     CDS             complement(1719..2078)
                     /gene="pspC"
                     /locus_tag="OO471_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6243696.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2078..2302)
                     /gene="pspB"
                     /locus_tag="OO471_002470"
     CDS             complement(2078..2302)
                     /gene="pspB"
                     /locus_tag="OO471_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6243697.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2356..3024)
                     /gene="pspA"
                     /locus_tag="OO471_002471"
     CDS             complement(2356..3024)
                     /gene="pspA"
                     /locus_tag="OO471_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6243698.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3191..4168
                     /gene="pspF"
                     /locus_tag="OO471_002472"
     CDS             3191..4168
                     /gene="pspF"
                     /locus_tag="OO471_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6243699.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4288..5553)
                     /gene="puuE"
                     /locus_tag="OO471_002473"
     CDS             complement(4288..5553)
                     /gene="puuE"
                     /locus_tag="OO471_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6243700.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5591..6871)
                     /gene="puuB"
                     /locus_tag="OO471_002474"
     CDS             complement(5591..6871)
                     /gene="puuB"
                     /locus_tag="OO471_002474"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6243701.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6873..8360)
                     /gene="puuC"
                     /locus_tag="OO471_002475"
     CDS             complement(6873..8360)
                     /gene="puuC"
                     /locus_tag="OO471_002475"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6243702.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8510..9067)
                     /gene="puuR"
                     /locus_tag="OO471_002476"
     CDS             complement(8510..9067)
                     /gene="puuR"
                     /locus_tag="OO471_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6243703.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9094..9858)
                     /gene="puuD"
                     /locus_tag="OO471_002477"
     CDS             complement(9094..9858)
                     /gene="puuD"
                     /locus_tag="OO471_002477"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6243704.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10070..11488
                     /gene="puuA"
                     /locus_tag="OO471_002478"
     CDS             10070..11488
                     /gene="puuA"
                     /locus_tag="OO471_002478"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6243705.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11637..11801
                     /locus_tag="OO471_002479"
     CDS             11637..11801
                     /locus_tag="OO471_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243706.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11791..13176
                     /gene="puuP"
                     /locus_tag="OO471_002480"
     CDS             11791..13176
                     /gene="puuP"
                     /locus_tag="OO471_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6243707.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13310..13555
                     /locus_tag="OO471_002481"
     CDS             13310..13555
                     /locus_tag="OO471_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6243708.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13868..15511
                     /gene="sapA"
                     /locus_tag="OO471_002482"
     CDS             13868..15511
                     /gene="sapA"
                     /locus_tag="OO471_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6243709.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15508..16473
                     /gene="sapB"
                     /locus_tag="OO471_002483"
     CDS             15508..16473
                     /gene="sapB"
                     /locus_tag="OO471_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6243710.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16460..17350
                     /gene="sapC"
                     /locus_tag="OO471_002484"
     CDS             16460..17350
                     /gene="sapC"
                     /locus_tag="OO471_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6243711.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17350..18342
                     /gene="sapD"
                     /locus_tag="OO471_002485"
     CDS             17350..18342
                     /gene="sapD"
                     /locus_tag="OO471_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6243712.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18344..19150
                     /gene="sapF"
                     /locus_tag="OO471_002486"
     CDS             18344..19150
                     /gene="sapF"
                     /locus_tag="OO471_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6243713.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19218..19571
                     /locus_tag="OO471_002487"
     CDS             19218..19571
                     /locus_tag="OO471_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6243714.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19939..20727
                     /gene="fabI"
                     /locus_tag="OO471_002488"
     CDS             19939..20727
                     /gene="fabI"
                     /locus_tag="OO471_002488"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6243715.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20871..21998
                     /locus_tag="OO471_002489"
     CDS             20871..21998
                     /locus_tag="OO471_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6243716.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22066..24000
                     /gene="rnb"
                     /locus_tag="OO471_002490"
     CDS             22066..24000
                     /gene="rnb"
                     /locus_tag="OO471_002490"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6243717.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24078..24181)
                     /locus_tag="OO471_002491"
                     /pseudo
     CDS             complement(24078..24181)
                     /locus_tag="OO471_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24235..25095
                     /locus_tag="OO471_002492"
                     /pseudo
     CDS             24235..25095
                     /locus_tag="OO471_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         5766 a         6559 c         6492 g         6278 t
ORIGIN      
        1 aactggtaca ccatatgcgc ttcgtcatca ccgttgccaa agtaagcaat gttgtctttg
       61 tgcggaacat tggtttcggt aataatcact gtacctggtg ctacgtcatc aataatcgac
      121 cgtaacagct tgataatcag atgtgttttt tccagatgga tgcagcttgt tcccggctct
      181 ttccacataa agccaacggc atccaggcgg acatactcag cacctttctc aaggtaacag
      241 agtaaaacat ccaccatcgc cagcaacact tcagggctac ggtaattcag gtcaatttga
      301 tcgtcactaa aggtggtcca taaatggcgc gtggaattat catccatttg aaacggcgtt
      361 aatagcggta acgcacgcgg aagggtgacg gcactgagat cggttttcgg atcaaccgca
      421 ataaagaaat cttcaaaacc cggaagctgt tgtaaatagt ttttaaacca ttcacttttt
      481 gcagacatat ggttgcagac aaaatcaaac attaaatgac tgcattcacc gagttgctga
      541 atatcctgcc actcccccgc ttcactggcg acctgatgat aatcaattac cgaaaatcca
      601 tcatcagatg accatgggta aaaaggtaat aaatgaacat gtgaaaaaat gctttgcagc
      661 cattgatggt agaactgatt aaatatgggt agtggtttta aatcattact gtgaaattga
      721 tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg tcgctgtgta
      781 attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt tgcggtaaat
      841 gttccaccgt agatttcgtc catgtaatcc gtaattttct gtttcatgtt gcctttccgt
      901 ggtagcggtc ccatgaggcg agagactact ccgcagaaaa taaactgtca attgagcaca
      961 agggttttag tcacgcagga aatcattttt taccagggaa aaagcgtatt tgtgagctga
     1021 cgcaaataat ctatagggat gtaaataagg tgtgacagaa aaaaaacggc gcataagcgc
     1081 cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt tcaggccacc
     1141 ggcgttctca acgtgggtat atcccatctc gctaaggagc tcttttgcct gcccggactg
     1201 gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacgg cggtggcaat
     1261 gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct cttgctgata
     1321 ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac
     1381 cagtgccaga gcaagtaagc ctttttttaa acatgtaagc gtccttctat ggatgaaaat
     1441 tgccatccat tatacgcctg agatttaaca atttttgcga aactcccctc accttttata
     1501 acgctgtgcc agtttattag cagcccgact taacagcggt tccagcgcca cagccagcaa
     1561 cattttcagc ggtcggcgag caactgattt tatcgcccag cccgccacac ccgccgggcc
     1621 atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaaaccaa gcttcacctt
     1681 ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg gaaacggcta
     1741 cgtaacgtga aagtatcgga agtgacataa cgttccatct cgcgtaaacg cgtttcactt
     1801 gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag ctgctcacca
     1861 aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta agcaaccagg
     1921 gtaaacagcg ccagaccgaa aaaaatcgac agcaccacca ggatacgcac cagttttacc
     1981 ggtacatcaa aatagttggc aatcccggcg cagacgccgc ggaccatgcc ctgctgtgga
     2041 atacgccata actttttatt gagattaatg cccgccatta gcgatccctc cagttcggat
     2101 gttctgcatc aagaatagat tccagcgcct gaatacgttc gcgcatccgt tttgcttcat
     2161 caaccagttg cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga ccagaacgat
     2221 tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg gttaacggaa
     2281 tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc gcgttcggac
     2341 gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt gtgccagttg
     2401 ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg atttttgttt
     2461 accgaagctg tggctttctg cttccgcttc catctggtca atacgacgtt cgaaagattc
     2521 gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca catcgcgcga
     2581 cgagtttgcc gcctgatgac gtaacatcaa tgcctgctgg cgagcgcgtg tttcgctcaa
     2641 tttgttttcc agctcgccaa tctctttctt catgcgtgcc agcgtatcgt ccaccagcgt
     2701 cacttcatgt tccagggact taatcagatc ggtcagtttc tgtttttcaa ttaacgctgc
     2761 acgtgccaga tcctctctct ctttcagcag cgccagttcg gctttttcct gccattcaac
     2821 ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg ccaacgcacg
     2881 cgccgaagta gaacgtactt caaccagtgt atcttccatc tcctggatca tcagacgcac
     2941 cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat tcacgatgtc
     3001 ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt caggattgtc
     3061 ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt tataaagatc
     3121 tgattgaaga atcaacagca acatgccagg atgaattagc gaattacact aacaagtggc
     3181 gaatttcatc atggccgaat acaaagataa tttacttggt gaggcgaaca gctttctcga
     3241 agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca tcatcggcga
     3301 acgcggcacc ggtaaagagt tgattgccag ccgcctgcat tatctctcct cccgttggca
     3361 agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg attccgaact
     3421 gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatcctg ggagatttga
     3481 acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga tgatggtgca
     3541 ggaaaaatta ttgcgtgtga ttgagtacgg tgaactggag cgcgttggcg gtagccagcc
     3601 gttgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg cgatggtcaa
     3661 tgaaggtact tttcgcgctg acctgctcga ccggctggct ttcgatgtag tgcaactgcc
     3721 gcccctgcgc gagcgcgaaa gcgacattat gctgatggca gaacactttg ccattcagat
     3781 gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg gagcgcgcca gagaaacatt
     3841 gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg aacgttcagt
     3901 gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc cctttaaacg
     3961 gcgtccacct gaagaagcca tcgccgtttc agaaaatacc tcgcttccga cgctgccgct
     4021 ggatttacgc gagtttcaga tgcagcagga aaaagagttg ctgcaactca gtttgcaaca
     4081 ggggaaatat aaccagaagc gcgcggctga attactgggg ttaacttatc atcagttccg
     4141 cgcgttgttg aaaaagcacc agatttagcg aagttgagca atacaacttg ccggatgcgg
     4201 cgtgaatgct ttattcggca tacataatca atacgtcgtt tgcaggcctg ataagcgcag
     4261 cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca aaattttcat
     4321 tgccgcatcg aattgcgcat ccgggatggt cagcggatac aggaagcgaa tcacgttgcc
     4381 gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct ggattttctg
     4441 tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta aactctgccg caatcatcga
     4501 ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa tcaacgtgtt
     4561 tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt cgataatatt
     4621 gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc
     4681 gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa gcggcatccc
     4741 gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat gatccatggc
     4801 aaacagttta ccggtacgcg caaagccgct ttgcacttca tcggcaatca tcacaatacc
     4861 gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa cgttgaaacc
     4921 gcactcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga
     4981 tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt gtaaatctga
     5041 cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt tgtacggcgc
     5101 aacttttccg gtcagcgcca tggtcatata tgtacgaccg tggaagccac cgctaaacgc
     5161 aatcacgcca ggtcgtccgg tatgggcgcg agcaattttc accgcgtttt ccaccgcttc
     5221 cgcgccggta gtgaagaatg cggttttagc ctgcccgctc actggggcaa gggtgttaat
     5281 tttctccgcc agcgtgacgt agctttcgta cggcacaatc tgatacgccg tgtgggtaaa
     5341 ctgttgcaat tgctgctcca ccgccgcgac cagatcaggg tggcgatgcc cggtattcag
     5401 caccgcaatg cctgcggcga aatcgatgta ttcgttgccc tctacgtcct tcagcgtagc
     5461 gttttctgcc gactgggcga agaagttaca catcacgcca accccgcgtg gggtagcaga
     5521 aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac tcttcactgt
     5581 taatcggaaa ttaaacgccc agacgatcgc gcaggctgta ataagccgcg cccatcgcgg
     5641 taaatggcac gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga ttggcgaagg
     5701 catcgaaacg ctcggcgtca ccgcgcagca gttcagcaat caaacgcccg gcaagatggg
     5761 tacaggtgac gccgtggccg ctgtaaccct gcatgtaata gatgttggta tcaaggcggc
     5821 cgaactgcgg catgtgcgac aaggtcagca ggaagttgcc cgtccagcgg taatcaattt
     5881 tcacgccctt caactgaggg aaggttttta gtagtttcgg taccacaaga cgctcaacgt
     5941 catccgggtt gcgtgcacca tacactacac ccccgccgta cagcaggcgg ttgtcggcgg
     6001 taagacggta gtaatcaagc agatagttac agtcttccac acagtagttt ttcgggatca
     6061 gcgaacgggc taaatcttcc gacagccgtt cggtggtgat cacctgcgtg ccgcacggca
     6121 tgctgcgttt cgctagttcc ggctctactt tatcgcccag atacgcattc ccggcgacaa
     6181 tcacatactt cgccgtcacc tgaccattgg cagtccgaac cacggctggc gtggtgtgct
     6241 ggatctgcgt cacggcggaa agctcataca cgcgcccgcc gttcaggcgg atggcatcgg
     6301 cttcaccaat ggcgaggttt agcgggtgaa tatgcccacc gctgtgatcc agcagcgcac
     6361 cagtgtagcg atcgctggcg acctcgcggc gaatagcgtt gacgtccagc agctccagct
     6421 gtttattgcc gtagcgtacc cagttctctt tctgctcttc gagtgtggcg agttgtttat
     6481 cattcatcgc cacaaacaga ccgccggggc ggtagtcgca gtcaatctga taacgtttga
     6541 tacgttcgcg gatgatctcg ccaccctcga acatcatgct gccgagcata cgggcggtgt
     6601 ccatgccgta gcttttttcg atcacgtcga tgtcgcggct gtaggagttc acaagctgcc
     6661 cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa
     6721 agcccgcttc cgccagatgc agcgcggagg agagcccggt atagccgccg ccaaccacgc
     6781 aaacgtcgca ggtgattgac tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg
     6841 cggcgtagta actgctggta tgttcggtca tttcaggcct ccaggcttat ccagatggtt
     6901 ttcagttcag tgaatttttc aagggcatgc agggatttgt cgcgaccgtt gccgctctgc
     6961 ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac gaagacggaa
     7021 ccggctttca ggcgtcggct catgcggtgc gcgcgggaga ggtcgcgcgt ccataccgcc
     7081 gcgccaaggc cgtactggct gtcgttggca agctgcagcg cctgatcttc tgatgtgaaa
     7141 cgggtgacca ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc attcgggtcc
     7201 acatccacga agatggtcgg gccgatggcc gctgccagcc cggcgttgcg accatccagc
     7261 agcagttgcc ccttgccttc gccttcacga ataaaggtgt gtaccgagtc agcgtgagcg
     7321 ctgtcgatta aggtgcccat ggtggttgcg ggatcaagtg gatggcccgg ctgccagttt
     7381 tgcgcctgct gttttaacag ggctaagaat tcatcggcga tgctctcttc cagtaacaaa
     7441 cgcgttccgg cgatgcgcac ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt
     7501 gccgcttttt gcagatccgg acaatcggcg aaaacgatgt tggcgctttt gccgcccgct
     7561 tccagccaga cacgtttcat gttgctatca cccgcgtctt tcagcagttg tttacccgta
     7621 cgcgtggagc cggtaaaggc gatggcatcg atatcgttat gacgcgaaag cgcctgcccc
     7681 gcttcatgac caaaacccgt caccacgttc aacacaccat ccggcaagcc tgcttctttc
     7741 gccagccccg cgagacgaat cgcactgagc ggtgattttt cagacggttt tagaatcacg
     7801 ctgtttcccg ccgccagcgc cgggccgagt ttccagcaag tcagcaacag cgggaagttc
     7861 cacggcacga tggcggcaat cacgccgacc ggttcacgca cgatcatcgc cagctcatgg
     7921 ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta ccagcgaatg
     7981 gcgcgcgccg cgccgggaat atcatcacgc agactgtgac gaatcggttt gccggtgtcg
     8041 agagtttcca gcagtgccag ctcttcggcg tgggcttcca ttaaatcggc gagtttattc
     8101 agtaccgctt tacgtttagc cggagaagag agtgaccagt cgccgcgttc aaatacgccg
     8161 cgtgctgcgc tcaccgcacg gtcgatatcg acgctcttgc cgcgggcaat tttcgccagc
     8221 ggtgcctggg tgaccggatc aacggtttca aaggtttcat tttccgccgc agcagtatat
     8281 tcaccgttaa taaataagcg gttttcaatg gcgagactta acgctttatc ctgccagtaa
     8341 gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat atgcagtttg
     8401 tgaacgtcag ggcgcagaga tccccgggct gcatgcccgt ttatttctgg cgacattctt
     8461 tatggattgc cgggcgcatt gcgcttaccc ggcttacaga acaaaaagat taaaacgtgg
     8521 tgggcgtatg ggcgctgata attcggcaaa tacctgccga cgtattactg aaactgtgcg
     8581 ggatgccggt attaatggca taactttgcc ccgcgacgag gtggtaatcc tgaccattaa
     8641 tcgtcagaac aatttcacct tccagtactg tgcctatttc ctcaccctga tgcttaattc
     8701 tttccccagt ggttgtgccc ggctggtacg tttcaaagat catcgccagc gtgcgattcg
     8761 ggttaccgtt atgaaccagc ttcattgaca caccctgact gcccatctca attaagtcgt
     8821 cctgattaat gacgacctgc ggctcatcag gtttttccgg ctcggaaaag aattccgaga
     8881 gtgacagacc atacaccttc agcagctttt gcagcgtact gatggcaggg ctgactttat
     8941 cttgttctat cgtactgata gcactgtgag tcagcccgga gagttcggcg gcacgacgtt
     9001 gtgaaagccc ctgctgctgg cggatttccg acaagcgttt tcctggcgcc agtccctcat
     9061 cactcatatt tgcatttcct taaactgtag tggtcagagt cgctgttttt cagcgatatg
     9121 gtgctgccaa gcggtgataa agccctcgaa caatatacgc gaaagcgcgt actcgctact
     9181 gttccattcc gggtgccact gtacgcccag cgcaaaagga tgattgatga cgctaaccgc
     9241 ctcaactaag ccatccggcg agcgagcttc aacacgcaac cgtgggctaa cgaccttcgc
     9301 cccttgtcca tgtagagagt ttacccaaaa gttgctacat tcaggtaaca acgcagacag
     9361 taatcccccc tcttcaacct gaacttcatg cgacggtgca tattgctgtt ccaccggcag
     9421 ttcgggatct tcccgatgtt ccagcaattc aggctgttcg cacagcttgc gatgcaacga
     9481 accaccggtt gccaccacca gttcttgtaa accccggcag atggcgaaaa tggggatgcg
     9541 cctttcgagt gcggcattaa ttatcgccat gctcagaaga tcacgcccgg gatcggcgtc
     9601 aggctcatcg ccgttttcac catataggtg cggctgcaca ttgctgggac tactaggaag
     9661 ataaatgcca tcgagtttcg gcaaaagttg ttcaagtaac gacggttccg ccagcgcatg
     9721 tggtagcgca attggcaagc cgcctgcgtg gatgatggca ttcaggtact tttcttgcag
     9781 agtctgggtc gcatgaccct taagcctgtt cctgcacatt acgacaccga taaccggatt
     9841 gttcattata ttttccatgc tcaatctcac aaaatggact aaattatcgc cattactgcg
     9901 ctttaaatga cctttatgtt caatattttt tcaatctagc agtggatttt catttttgca
     9961 aactcaattt aacatttgac aaacatttgg tttgcataca gattcgaatg atggtcatta
    10021 tattttacgc tttgataacg agcggaaaac aaaccaaagg cgaagaatca tggaaaccaa
    10081 tatcgttgaa gtagagaact ttgttcagca gtcagaagag aggcggggta gcgcctttac
    10141 gcaggaagtg aaacgctacc tggagcgcta cccgaatacg caatatgttg atgttttact
    10201 tactgatttg aatggttgct tccgtggaaa acgtatccct gtttcaagcc tgaagaagct
    10261 cgagaaaggg tgttatttcc cggcctcggt atttgcaatg gatattctgg gcaacgtagt
    10321 agaagaagca ggtctgggtc aggaaatggg cgagccggat cgtacctgtg ttcctgttct
    10381 cggttcctta actccttctg ccgccgatcc agagtttatc ggtcagatgc tcctgaccat
    10441 ggtcgatgaa gatggcgctc cctttgacgt tgagccgcgg aacgttctta accgcctgtg
    10501 gcagcagctg cgccagcgcg gattgttccc ggttgtagcg gtagagctgg agttctattt
    10561 actggatcgc cagcgcgacg ctgaagggga tctgcaaccg ccctgcgcgc ctggcaccga
    10621 tgaccgcaat acacaaagcc aggtttactc cgttgataac ctcaaccact tcgccgacgt
    10681 gctcaatgat attgatgaac tggcgcagtt gcagctgatt ccggcagatg gcgcggtcgc
    10741 tgaggcctcg ccgggtcagt ttgaaatcaa cctttaccat actgataacg tgctggaagc
    10801 ctgcgatgat gcgctggcac taaaacgcct agtgcgtctg atggcagaaa agcataagat
    10861 gcacgccact tttatggcga agccgtatga agagcacgcg ggcagcggga tgcatatcca
    10921 tatcagtatg caaaataatc gtggcgagaa tgtgctttct gacacagaag gcgaagattc
    10981 gccgctgctg aaaaagatgc tcgccgggat gattgacctg atgccgtcgt cgatggcgtt
    11041 gctggcacca aacgtgaact cgtatcgccg cttccagccg ggaatgtatg tgccgacgca
    11101 ggcgtcgtgg ggccataaca accgcaccgt cgccctgcgt attccgtgcg gtgaccgtca
    11161 taatcaccgc gtggaatatc acgtggcggg tgccgatgcc aacccatatc tggtgatggc
    11221 agcgattttt gccggtattt tgcatggcct tgataacgag ctgccgttgc aggaagaagt
    11281 cgaaggcaac gggctggaac aggaaggctt acccttcccg attcgccaga gcgatgccct
    11341 gggtgagttt atcgagaatg atcacctgcg ccgctattta ggcgaacggt tttgccatgt
    11401 gtatcacgcc tgtaaaaatg atgagctgtt gcagtttgag cgcctcatca cagaaacaga
    11461 aattgagtgg atgttgaaaa acgcgtaatt ccccttgcgg ggttgtttca tcggcctgag
    11521 cggagccagg cctgacattt gctggtaatc cagacatttc ccggatgtcg cgttatggag
    11581 cgcaggcggc aacgggcttc cgttaacgat taagactgtg acgaggaaat gagatgatgc
    11641 cgatgattaa atccccgcat ggcgagggag gctgcgtatg cgcccctcct gctactgact
    11701 ggactccgcc ccctttgctt cccttgctaa acaggtttga tttcaggtcg acacgaccgc
    11761 aaaccttatt acgcagggga ggcagcaatt atggctatta attcaccact gaatattgct
    11821 gcgcaacccg gcaaaacccg tctgcgaaaa tcactgaaat tgtggcaggt ggtaatgatg
    11881 ggtctggcct atctcacgcc gatgaccgta tttgatactt ttggtattgt ctccggcatt
    11941 agcgacggtc acgttccggc gtcctatttg ctggcgctgg cgggcgtgct gtttaccgct
    12001 atcagctacg gcaaactggt tcgccagttt ccggaggccg gttcggccta tacctacgcg
    12061 caaaagtcga ttaacccgca cgtcggattt atggtcggct ggtcatcact gctggattat
    12121 ctctttttgc caatgatcaa cgttttgttg gcgaaaatct atctctccgc cctcttcccg
    12181 gaagtgccgc cgtgggtgtg ggtggtaacc ttcgtcgcca ttttaaccgc cgcgaatctg
    12241 aagagcgtca acctggtcgc taacttcaat accctgtttg tactggtgca aatctccatc
    12301 atggtggtgt ttatcttcct ggtggttcag ggactgcata aaggagaagg cgttggcacc
    12361 gtctggtcac ttcagccgtt tatcagcgag aacgcgcacc tgatcccgat tattaccggt
    12421 gcgacgattg tctgtttctc gttcctcggt ttcgatgcgg tgaccacgct ttcggaagag
    12481 acgccggacg ccgcacgcgt gatcccgaaa gccatcttcc tgacggcggt atacggcggc
    12541 gttatcttta tcgcggcgtc gttctttatg cagctgttct ttcccgatat cagccgcttt
    12601 aaagacccgg acgccgcact gcctgaaatt gcgctgtacg tcggcggcaa gctctttcag
    12661 tcgattttcc tctgcaccac gtttgtgaac acattagcgt ctggcctggc ttcacatgcc
    12721 agcgtgtcgc gtctgctgta tgtgatgggg cgtgacaatg tgtttccgga gcgcgtgttt
    12781 ggctatgtgc acccaaaatg gcggactccg gcactcaacg tcattatggt cgggattgtc
    12841 gcgctgtcgg cgctgttctt tgatttagtc accgcgacag cactgattaa cttcggtgct
    12901 ctggtggcgt ttaccttcgt gaatctgtcg gtgtttaatc atttctggcg gcgtaaagga
    12961 atgaataaaa gctggaagga tcacttccac tatttgctga tgccgctggt tggcgcgctg
    13021 acggtgggtg tgctgtgggt taacctagag tcaacgtcac tgacactcgg tctggtatgg
    13081 gcttcgttag gcggcgcata tttgtggtat ctgatccgcc gctatcgcaa agtgccgctg
    13141 tacgaaggtg acagaacgcc ggtgagcgaa acgtaatagc cggatgcgcg gcgcattatc
    13201 ctgcctgagg gtaacgcgcc gcgccattaa ctataaaaag atgtatgacc cggatcacga
    13261 gtgctatcat ccgcccttat gcttagcgca ttaaccccag gggcaaataa tgaaccacga
    13321 tatcccgcta aaatattttg atattgccga tgagtacgcg accgaatgcg cagagcctgt
    13381 cgcagaggct gaacgcacac cgctggccca ctacttccag ttactgctca cccgtttgat
    13441 gaacaatgaa gagatcagcg aagaagccca acatgaaatg gctgctgaag cggggattaa
    13501 tcccgtacgc attgatgaga tcgcagagtt tttgaatcaa tggggtaatg agtaattatt
    13561 taattgcaga cgatcggtca gtatcgttgt ccggataaag cgttcacgcc gcatccggct
    13621 aacaatgaca cgcactgcag cgtgctttaa tgggtttctc acgatcgtac cgctctgctc
    13681 ctcgctaagt tgttctgtga gttatagtta tagagaaatc acgggtacga atgttgaaca
    13741 gatggcgacg ccacctgttc aacgaccaac cacacggccc tcattacgac ccctgaacac
    13801 aaacggtaat ggcaaatgtc attaaatatt agtaagatag cctctttaca taaaatccct
    13861 taatattatg cgccaggtat tatcgtctct tttggtgatt gctggacttg tgagtggtca
    13921 ggcaatcgcc gcgcctgaat cccccccgca tgctgatatc cgcgacagcg gttttgtcta
    13981 ttgcgtcagc gggcaagtca acacctttaa cccatccaaa gcgagcagtg ggttaattgt
    14041 cgataccctt gccgcccagt tttatgatcg actgctggat gtcgatccct atacctatcg
    14101 cctgatgccg gaacttgccg aaagctggga agtactcgac aacggcgcga cctatcgctt
    14161 ccacctgcgt cgcgatgttc cgtttcaaaa aaccgactgg tttactccca ctcgtaaaat
    14221 gaatgccgac gatgtggtgt ttaccttcca gcgaattttt gaccgcaaca acccgtggca
    14281 taacgtcaac ggcagcaact tcccctactt cgacagcctg caatttgccg ataacgtgaa
    14341 aagcgtccgc aaactggata atcataccgt tgagttccga ctcgcccagc cggatgcttc
    14401 ttttttgtgg cacctcgcaa cccattatgc ttcggtcatg tcggcagaat atgcccggaa
    14461 gttagagaaa gaagatcgcc aggagcaact cgaccgtcaa ccggtcggca ccggtccgta
    14521 tcagttgtcg gaataccgcg ccgggcaatt tattcgccta caacgtcatg atgacttctg
    14581 gcgcggtaaa ccgttaatgc cgcaggtagt ggtggattta ggctccggcg gcaccggacg
    14641 tctgtcgaaa ctcctgaccg gggaatgcga cgttctggcc tggcctgctg ccagccagct
    14701 atccattttg cgtgacgacc cgcgcttgcg tttaacgctg cgtcctggga tgaacgtcgc
    14761 ctatctggca tttaacaccg ccaaaccgcc gctaaataat cccgctgtcc gccatgcact
    14821 ggcactggcg attaataacc agcgcctgat gcaatccatc tattatggta cggctgaaac
    14881 ggcggcctct attttaccgc gcgcctcgtg ggcctatgac aacgaggcta aaattactga
    14941 atacaatccg gcgaaatcgc gcgaacagtt gaaggcgttg gggctggaaa acttaacgct
    15001 gaaactgtgg gtgcccacac gttcgcaggc gtggaacccc agtccactga aaactgccga
    15061 actgattcag gcggatatgg cgcaggttgg cgtaaaagtg gtgattgtgc cggtagaagg
    15121 tcgctttcag gaggcgcggt tgatggatat gagccatgat ctgacgttat ccggttgggc
    15181 gacggacagt aacgacccgg acagtttctt ccgtccgtta ctgagctgcg cggcaattca
    15241 ttcacagacc aacctcgccc actggtgcaa tccgaaattc gacagcgtgt tgcgtaaggc
    15301 gctctcctcg cagcagctgg cagcgcgtat tgaagcctat gacgaagcgc agagtattct
    15361 ggcgcaggaa ttacctattc tgccgctggc gtcgtcattg cgtttgcagg cctatcggta
    15421 cgatatcaaa ggtctggtac ttagcccgtt tggtaacgcc tcctttgctg gggtgtatcg
    15481 cgagaaacag gatgaggtga aaaaaccatg attatcttca ccttacgccg cattttgtta
    15541 ttgattgtca ccttgttcct gctgaccttc gttggcttca gcttgagcta tttcactcca
    15601 cacgcgccgt tacaaggcgc gtcactgtgg aatgcctggg tgttctggtt taatggcctg
    15661 atccactggg attttggtgt ttccagcatt aacggtcaac caattgccga acagttaaaa
    15721 gagctcttcc cggcgacgat agaactgtgc atcctcgcct tcggttttgc gctgattgtt
    15781 ggcatccccg tggggatgat tgccgggatt acgcgccata agtggcagga taacttgatc
    15841 aacgccatcg ccttactggg cttttcaatc ccggtgttct ggctggcgct tctgttgaca
    15901 ctgttttgtt cactcacgct aggttggttg cccgtttcag ggcgtttcga tctgctctac
    15961 gaagtgaaac cgattaccgg ttttgcgttg attgatgcct ggctttcgga ctcaccatgg
    16021 cgggatgaaa tgatcatgag cgcaatccgc catatgatat tgcccgtgat caccctgtcg
    16081 gttgcgccaa caactgaagt gatccgcctg atgcgtatca gcactattga agtgtatgac
    16141 cagaactatg taaaagcggc ggcgacccgc ggtttgtcgc gctttactat tttgcgtcgc
    16201 cacgttctgc ataacgcgtt accgccggtt attcctcgcc tgggcttaca gttttccacc
    16261 atgctgacgc tggcgatgat caccgaaatg gtttttagct ggccgggttt agggcgctgg
    16321 ttaattaacg ccatccgcca gcaggattac gcagccattt ccgccggagt gatggtgtgt
    16381 ggctcactgg tcattattgt taacgtgatt tctgatattt tgggtgccat ggctaaccct
    16441 ctgaaacata aggaatggta tgccttacga tagcgtatac agcgaaaagc gcccgccggg
    16501 cacgctgcgt accgcctggc gcaaatttta tagtgacgcc tctgcgatgg tcggcctgta
    16561 cggctgcgcg ggactggctg tactgtgtat ttttggcggc tggtttgcgc cttacggcat
    16621 cgaccagcaa tttctcggtt atcaattatt gccgccatca tggtcgcgct atggcgaagt
    16681 ttctttcttc ctggggactg acgacctcgg acgtgatgtt ttaagccgtt tactgagcgg
    16741 agctgcgcca accgtgggtg gcgcatttgt ggtgacgctt gccgcgacga tctgtggcct
    16801 ggtactgggc accttcgccg gggcgacaca cggcctccgc tcagcggtgc ttaaccatat
    16861 tctggatacc ttactggcga tcccttcgct gctgttggca attatcgttg tagcgtttgc
    16921 cggaccgagt ttgtcccacg ctatgtttgc tgtctggctg gcattgctgc cgcgtatggt
    16981 acgttcgatt tacagcatgg tgcatgacga actggaaaaa gagtacgtta tcgccgcccg
    17041 tctggatggc gcatcaacgc tgaatattct ctggtttgct gtgatgccaa acatcaccgc
    17101 cgggctggtc accgagatca cccgcgcact gtcgatggca attctcgata tcgccgcgct
    17161 gggctttctc gatctcggcg cacaactccc ctcgcctgaa tggggagcga tgctcggtga
    17221 tgcgctggaa ctgatttatg tcgccccgtg gactgtcatg ctgccaggtg cggcaattat
    17281 gattagcgtg ttgttggtta acctccttgg cgatggcgtt cgtcgtgcga ttattgcggg
    17341 ggtggaataa tgccattact cgatattcgt aacctgacca ttgaatttaa aaccggtgat
    17401 gagtgggtta aagccgtcga ccgggtaagt atgaccttaa ccgaaggtga aatccgcggt
    17461 cttgttggtg aatccggttc cggcaagagt ttgattgcga aagcgatttg tggggtgaat
    17521 aaagataact ggcgtgttac tgctgaccgt atgcgttttg atgatatcga tttgctgcgt
    17581 ctctccgcac gcgaacggcg caaactggtt ggccataacg tgtcgatgat tttccaggaa
    17641 ccgcagtcgt gtcttgaccc ttcagaacgt gtgggtcgcc agttgatgca aaacatccca
    17701 gcctggacct ataaaggccg ttggtggcag cgttttggct ggcgcaaacg ccgtgccatt
    17761 gaactgctgc accgcgtggg gattaaagat cacaaagatg cgatgcgcag ttttccctat
    17821 gagttgaccg aaggtgaatg tcagaaagtg atgatagcca ttgcactggc gaatcaaccg
    17881 cgtctgctga ttgctgacga accgacaaac tcaatggagc caacaaccca ggcgcaaatc
    17941 tttcgcctgc tgacgcgtct caaccaaaac agcaatacca ctattttgct tatcagccat
    18001 gacttacaaa tgcttagcca atgggcggat aaaattaacg tgctttactg cggtcaaaca
    18061 gtggaaaccg cgccgagtaa ggagttggtg acgatgccac atcatcctta tacccaggcg
    18121 ctgatccgcg cgataccaga cttcggcagc gcgatgccgc ataaaagtcg cctcaatact
    18181 ctacccggcg ctatcccatt gctggaacag ttaccgattg gttgtcgtct ggggccacgt
    18241 tgcccgtatg cacaacgaga atgcattgtg acgccacgtt tgacgggggc gaaaaatcat
    18301 ctctatgcct gtcatttccc gctgaacatg gagaaagagt gagatgatcg aaacgctgct
    18361 tgaagtgcgt aatttaagta aaacgttccg ctaccggacc ggttggtttc gtcgtcagac
    18421 cgtagaagcg gtaaaaccct tgagctttac gctacgtgaa ggccagacac tggcgattat
    18481 tggcgagaat ggttcgggta aatccacact ggcaaaaatg ctggcgggaa tgatagaacc
    18541 taccagcggc gagttattga ttgacgatca tccgctgcat tttggcgatt attccttccg
    18601 tagtcagcgt attcgcatga tttttcagga tccttcgacc tcgttgaatc cccgtcaacg
    18661 tatctcacaa attctggatt ttccactgcg cctgaacacc gacctggaac cggaacagcg
    18721 tcgcaaacag attattgaaa cgatgcgtat ggttgggctg ctaccggatc acgtcagtta
    18781 ctatccgcat atgttggcac ccggacaaaa acagcgtctg ggtctggcgc gcgcgctgat
    18841 attgcgccca aaagtcatta ttgccgatga agccctcgct tcactggata tgtcaatgcg
    18901 ttcgcagttg attaatctga tgctggagtt acaggaaaaa cagggcattt cgtatattta
    18961 tgttacccag catatcggaa tgatgaagca cattagcgac caggtgctgg tgatgcatca
    19021 gggcgaggtt gtcgagcgag gcagcaccgc ggatgtgctg gcatcgccgc tgcatgaact
    19081 caccaaacgg ctgattgccg ggcactttgg cgaagcattg acagcggatg cgtggcgtaa
    19141 agatcgctaa ttttctgaat cctccttcca gattattcac ctcattctga caagagatta
    19201 aatcttgtca gaatgaggtg atggataaaa taaaatcaaa tgcgcgtgat ttaagacgca
    19261 atctcacttt gcaggaacga aagctctggc gatatcttcg cagccgacgt tttggtgatt
    19321 tcaaatttcg ccgtcaacat ccggtgggga gctacattct cgattttgct tgctgctcgg
    19381 cgcgcgtagt cgttgagctg gatggtgggc agcatgattt ggcagttgcc tatgatacca
    19441 ggcgcactag ctggcttgag tcgcagggct ggaccgtgct gcgtttctgg aataacgaga
    19501 ttgattgtaa tgaggaggcg gtgctggaga ttattctgca ggaactgaac cgccggtcac
    19561 cctcaccctg aaagggcgag ggggccgacc gagccgaata gctgttgtgg tgaaaacatg
    19621 gagacagtgc tggagaatat tcggcaaggt ctgaaccatc ccagccatcg ccataaaagg
    19681 gttaggggct gtatgagcct gtttgttgct ggggtaacaa tatttgcaca atatggtccc
    19741 ctcgcccctc tggggagagg gttagggtaa ggggaaaagc gccccccctg ccgcagcctg
    19801 ctccggtcgg acctggtaac tatagctact cacagccagg ttgattataa taaccgttta
    19861 tctgttcgta ctgtttacta aaacgacgaa tcgcctgatt ttcaggcaca acaagcatca
    19921 acaataagga ttaaagctat gggttttctt tccggtaagc gcattctggt aaccggtgtt
    19981 gccagcaaac tgtccatcgc ctacggtatc gctcaggcga tgcaccgcga aggagctgaa
    20041 ctggcattca cctaccagaa cgacaaactg aaaggccgcg tagaagaatt tgccgctcaa
    20101 ttgggttctg acatcgttct gcagtgcgat gttgcagaag atgccagcat cgacaccatg
    20161 ttcgctgaac tggggaaagt ttggccgaaa tttgacggtt tcgtacactc tattggtttt
    20221 gcacctggcg atcagctgga tggtgactat gttaacgccg ttacccgtga aagcttcaaa
    20281 attgcccacg acatcagctc ctacagcttc gttgcaatgg caaaagcttg ccgctccatg
    20341 ctgaatccgg gttctgccct gctgaccctt tcctaccttg gcgctgagcg cgctatcccg
    20401 aactacaacg ttatgggtct ggcaaaagcg tctctggaag cgaacgtgcg ctatatggcg
    20461 aacgcgatgg gtccggaagg tgtgcgtgtt aacgccatct ctgctggtcc gatccgtact
    20521 ctggcggcct ccggtatcaa agacttccgc aaaatgctgg ctcattgcga agccgttacc
    20581 ccgattcgcc gtacagttac tattgaagat gtgggtaact ctgcggcatt cctgtgctcc
    20641 gatctctctg ccggtatctc cggtgaagta gtccacgttg acggcggttt cagcatcgct
    20701 gcaatgaacg aactcgaact gaaataatcg ttctgttggt aaagatgggc ggcgttctgc
    20761 cgcccgttat ctctgttata cctttctgat atttgttatc gccgatccgt ctttctcccc
    20821 ttcccgcctt gcgtcaggat aacgatttcc tttacgacca aggagcgccc atggaacaac
    20881 gccacatcac cggcaaaagc cactggtatc atgaaacgca atccagtact acggagtatg
    20941 acgttctgcc tctggtcccg gaagccgcaa aggtcagcga tccctttctg ctcgacgtga
    21001 tccttgatga agagacgctg gcccccttcc tttcatggct ggtccctgcg cgcgttcttg
    21061 cagtggagtt gttccctgac cagcttaccg tgacccgttc acaaaccttc accgcttatg
    21121 aacgcttgtc gacggccctg acggttgctc aggtttgcgg cgtccagcgg ttatgtaact
    21181 actattcggc gcgacttacg ccgctccccg gacctgattc ctccagggaa agtaatcatc
    21241 ggttggcaca aatcacgcaa tatgcccgcc aactggctag ctcgccttct attatcgaca
    21301 accgatcgcg ccagcatctg aatgacgtcg gtcttactgt ctgggactgt gtgatcatta
    21361 accaaatcat tggttttatt ggctttcagg cgcggacaat tgcgacattt caggcttatc
    21421 tcgggcatcc ggtacgctgg ttacccgggc tggcgataca aaactacgcc gacgcgtcac
    21481 tgtttgctga tgaatcaata cgctggcgaa gcagctatga ggtggaaaaa ctacctgaag
    21541 agcacacaaa aagttcaact gcagaacttt gccaactggc cgaaacactc tctctccacc
    21601 ctatttcact ttcccttctc gaaaagttgt taaacagcac acgggtcaat gcacagccgg
    21661 ataatcagct tgcggcgttg ttatgcgcgc gtataaatgg cagtcctgct tgtttttcca
    21721 cctgtatgga ttcatcaaat gaatataaaa aaatcagcac ccttctgcgc aagggcgaaa
    21781 atgaaattaa ccgatgggct gaccgtcatt ctgttgagcg cgctaccgtt caggcgatac
    21841 aatggctgac ccgagcaccc gatcgcttta gcgccgccca gttcagccct ttactcgaac
    21901 acgaaaaatc atcaacgcag attattaatc tgctggtatg gagcgggctg tgtggctgga
    21961 taaatcgctt aaaaatcgcg ttgggtgaga catattaacc ttgccgcgtc agacagattc
    22021 gcgtaaaact gtcagccgct ctaatggcca ccaaaataga caattatgtt tcaggacaac
    22081 ccgctgctag cgcagcttaa acagcaactg cattcccaga cgccacgcgc tgaaggggtg
    22141 gtaaaagcca cagaaaaagg ctttggcttc ctggaagtcg acgcacaaaa aagttatttc
    22201 attccgccgc cgcagatgaa aaaagtcatg catggcgacc gaattatcgc ggtgatccac
    22261 agtgaaaaag aacgtgaatc cgcagagcca gaagaactgg ttgaaccgtt cctgactcgt
    22321 ttcgtgggta aagttcaggg caaaaatgac cgtctgacca tcgttcctga tcatccactt
    22381 ttaaaagacg ccattccttg ccgcgcagcc cgtggcctga accacgagtt taaagaaggc
    22441 gactgggcgg ttgccgaaat gcgccgtcat ccgctgaaag gcgatcgttc tttctatgct
    22501 gaactgacac aatacatcac ttttggtgac gaccactttg taccgtggtg ggttaccctt
    22561 gcgcgccata atctggaaaa agaagcacca gacggcgtcg ctaccgaaat gctcgatgaa
    22621 ggtctggttc gtgaagatct gacctcgctg gattttgtca ccatcgacag tgccagcact
    22681 gaagatatgg atgacgccct tttcgctaag gcgctgccgg atgacaaact tcagctgatt
    22741 gtggcgattg ccgatccaac cgcgtggatt gctgaaggca gcaagctgga caaagccgcg
    22801 aaaattcgcg cattcaccaa ctatctgcct ggcttcaaca tccctatgct gcctcgcgag
    22861 ctttctgacg atctctgctc actgcgcgcc aatgaagtcc gcccggtact ggcatgccgc
    22921 atgacgctct ccgctgatgg caccattgaa gataatatcg aattctttgc cgccaccatc
    22981 gaatccaaag cgaagctggt gtatgaccag gtttctgact ggctggaaaa taccggtgac
    23041 tggaagcctg aaagtgaagc aattgccgaa caagtccgtt tgctagcgca aatttgccaa
    23101 cgccgcggcg agtggcgtca taaccacgca ctggtgttta aagatcgccc ggattaccgc
    23161 tttattctcg gtgaaaaagg tgaagtgctg gatatcgtcg ccgagcctcg tcgcattgcc
    23221 aaccgtatcg tcgaagaagc gatgattgcc gctaacattt gtgcggcccg cgtactgcgc
    23281 gataagctcg gttttggcat ctataacgtg catatgggct ttgatccggc gaatgccgac
    23341 gcgctggcag cgttgctgaa aacgcacggt ctgcatgtcg atgccgaaga agtgctcacg
    23401 ctggacggtt tctgcaaact gcgtcgtgaa ctggatgcgc aaccaactgg tttcctcgac
    23461 agccgcattc gtcgcttcca gtcatttgct gaaattagct ctgaacccgg tcctcacttt
    23521 ggcctcggtc tggaagcata cgccacctgg acttcgccga tccgtaaata tggcgacatg
    23581 atcaaccacc gtctgctgaa agcggttatc aaaggcgaaa ctgcgacgcg tccgcaggat
    23641 gaaatcactg tccaaatggc cgaacgtcgc cgtctcaacc ggatggcaga acgtgatgtt
    23701 ggcgactggt tatacgcacg cttcctgaaa gataaagccg ggaccggcac ccgtttcgcg
    23761 gcggaaattg tcgatatcag ccgtggcggc atgcgtgtgc gtttggttga taacggcgct
    23821 atcgccttta ttccggcacc tttcttacac gctgtgcgcg atgaactggt ttgcagccag
    23881 gaaaacggca ctgtacaaat taaaggtgaa acggtttaca aagtaactga cgttattgac
    23941 gtcaccattg ccgaagtccg catggaaacc cgcagcatta ttgcgcgccc ggtcgcgtaa
    24001 tctcctttca cggcccattc ctcatggatg ggccgtttat ttccccgctc taccttcgtt
    24061 atatccaggc agatttttta actgactttc gtttgaaaac tggcgttttt ccaaaactgg
    24121 attacaaata aatatataaa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
    24181 tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg catcatgaaa
    24241 accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc atactggcgg
    24301 ttgacggaaa gcagcgatgc tttgcgcttt tctaccacca aaaccacaga acctgatcgt
    24361 acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt catcacctcc
    24421 agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga
    24481 aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac accggcggtt
    24541 gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga agcacattcc
    24601 gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt atgtgaagat
    24661 tacacagggt tgaaagaaca cgacgtcatt gggtaaagcg tgtttaaact gtttatgagc
    24721 cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag cggcaatgca
    24781 tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct gtttcgcaat
    24841 aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac
    24901 attaccgaag agcgccgcac tcaggagcga ctgcgtattc tggcaaatac cgacagtatc
    24961 accggactgc cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca
    25021 gataacaata aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac
    25081 gcctatgggc atttg
//
LOCUS       DAJTUM010000058        24183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTUM010000058 DAJTUM010000000
VERSION     DAJTUM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(22..594)
                     /locus_tag="OO471_002493"
     CDS             complement(22..594)
                     /locus_tag="OO471_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6243718.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO471_002494"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO471_002494"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6243719.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO471_002495"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO471_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6243720.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO471_002496"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO471_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6243721.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO471_002497"
     CDS             complement(3556..5337)
                     /locus_tag="OO471_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6243722.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO471_002498"
     CDS             complement(5330..7102)
                     /locus_tag="OO471_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6243723.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO471_002499"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO471_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6243724.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO471_002500"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO471_002500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6243725.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO471_002501"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO471_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO471_002502"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO471_002502"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6243726.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO471_002503"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO471_002503"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6243727.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO471_002504"
     CDS             complement(11658..12029)
                     /locus_tag="OO471_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6243728.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO471_002505"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO471_002505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6243729.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO471_002506"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO471_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6243730.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO471_002507"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO471_002507"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6243731.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO471_002508"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO471_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6243732.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO471_002509"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO471_002509"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6243733.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO471_002510"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO471_002510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6243734.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO471_002511"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO471_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6243735.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO471_002512"
     CDS             21800..22378
                     /locus_tag="OO471_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6243736.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO471_002513"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO471_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6243737.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6664 c         5827 g         6025 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 ata
//
LOCUS       DAJTUM010000059        24089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTUM010000059 DAJTUM010000000
VERSION     DAJTUM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="OO471_002514"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="OO471_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6243738.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO471_002515"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO471_002515"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6243739.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="OO471_002516"
     CDS             1531..2478
                     /locus_tag="OO471_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6243740.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="OO471_002517"
     CDS             2548..3354
                     /locus_tag="OO471_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6243741.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="OO471_002518"
     CDS             3364..3771
                     /locus_tag="OO471_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6243742.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="OO471_002519"
     CDS             3771..4265
                     /locus_tag="OO471_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6243743.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="OO471_002520"
     CDS             4331..5128
                     /locus_tag="OO471_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6243744.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="OO471_002521"
     CDS             5139..5927
                     /locus_tag="OO471_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6243745.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="OO471_002522"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="OO471_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6243746.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="OO471_002523"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="OO471_002523"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6243747.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="OO471_002524"
     CDS             complement(8370..10001)
                     /locus_tag="OO471_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6243748.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO471_002525"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO471_002525"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6243749.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="OO471_002526"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="OO471_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6243750.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="OO471_002527"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="OO471_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO471_002528"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO471_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO471_002529"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO471_002529"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6243751.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO471_002530"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO471_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO471_002531"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO471_002531"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6243752.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="OO471_002532"
     CDS             23609..24052
                     /locus_tag="OO471_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6243753.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5943 a         6148 c         6108 g         5890 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatc ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTUM010000060        23932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTUM010000060 DAJTUM010000000
VERSION     DAJTUM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..386)
                     /locus_tag="OO471_002533"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO471_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO471_002534"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO471_002534"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6243754.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO471_002535"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO471_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6243755.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO471_002536"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO471_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6243756.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO471_002537"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO471_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6243757.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO471_002538"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO471_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6243758.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO471_002539"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO471_002539"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6243759.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO471_002540"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO471_002540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6243760.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO471_002541"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO471_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6243761.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO471_002542"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO471_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6243762.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO471_002543"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO471_002543"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6243763.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO471_002544"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO471_002544"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6243764.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO471_002545"
     CDS             10748..11212
                     /locus_tag="OO471_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6243765.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO471_002546"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO471_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6243766.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO471_002547"
     CDS             12236..13672
                     /locus_tag="OO471_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6243767.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO471_002548"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO471_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6243768.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO471_002549"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO471_002549"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6243769.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO471_002550"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO471_002550"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6243770.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO471_002551"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO471_002551"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6243771.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO471_002552"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO471_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6243772.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO471_002553"
     CDS             complement(20505..20798)
                     /locus_tag="OO471_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6243773.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO471_002554"
     CDS             complement(20795..22084)
                     /locus_tag="OO471_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6243774.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO471_002555"
     CDS             complement(22140..23078)
                     /locus_tag="OO471_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6243775.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO471_002556"
     CDS             complement(23098..23862)
                     /locus_tag="OO471_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6243776.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5910 a         5803 c         6302 g         5917 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cg
//
LOCUS       DAJTUM010000061        22427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTUM010000061 DAJTUM010000000
VERSION     DAJTUM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            83..2683
                     /locus_tag="OO471_002557"
     CDS             83..2683
                     /locus_tag="OO471_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6243777.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2674..3657
                     /locus_tag="OO471_002558"
     CDS             2674..3657
                     /locus_tag="OO471_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6243778.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3755..4295
                     /locus_tag="OO471_002559"
                     /pseudo
     CDS             3755..4295
                     /locus_tag="OO471_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4303..4818
                     /locus_tag="OO471_002560"
     CDS             4303..4818
                     /locus_tag="OO471_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6243779.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4784..5521
                     /locus_tag="OO471_002561"
     CDS             4784..5521
                     /locus_tag="OO471_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6243780.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5632..6642
                     /gene="pyrD"
                     /locus_tag="OO471_002562"
     CDS             5632..6642
                     /gene="pyrD"
                     /locus_tag="OO471_002562"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6243781.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6816..7358
                     /gene="zapC"
                     /locus_tag="OO471_002563"
     CDS             6816..7358
                     /gene="zapC"
                     /locus_tag="OO471_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6243782.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO471_002564"
     CDS             complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO471_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6243783.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO471_002565"
     CDS             8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO471_002565"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6243784.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10828..12735
                     /locus_tag="OO471_002566"
     CDS             10828..12735
                     /locus_tag="OO471_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6243785.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12865..14118
                     /gene="pqiA"
                     /locus_tag="OO471_002567"
     CDS             12865..14118
                     /gene="pqiA"
                     /locus_tag="OO471_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6243786.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14123..15763
                     /gene="pqiB"
                     /locus_tag="OO471_002568"
     CDS             14123..15763
                     /gene="pqiB"
                     /locus_tag="OO471_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6243787.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15760..16323
                     /gene="pqiC"
                     /locus_tag="OO471_002569"
     CDS             15760..16323
                     /gene="pqiC"
                     /locus_tag="OO471_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6243788.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16579..16746
                     /gene="rmf"
                     /locus_tag="OO471_002570"
     CDS             16579..16746
                     /gene="rmf"
                     /locus_tag="OO471_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6243789.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO471_002571"
     CDS             complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO471_002571"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6243790.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17403..19163)
                     /locus_tag="OO471_002572"
     CDS             complement(17403..19163)
                     /locus_tag="OO471_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6243791.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19348..19800
                     /gene="matP"
                     /locus_tag="OO471_002573"
     CDS             19348..19800
                     /gene="matP"
                     /locus_tag="OO471_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6243792.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO471_002574"
     CDS             complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO471_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6243793.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO471_002575"
     CDS             complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO471_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6243794.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22006..>22427
                     /gene="sxy"
                     /locus_tag="OO471_002576"
     CDS             22006..>22427
                     /gene="sxy"
                     /locus_tag="OO471_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6243795.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGRLAP"
BASE COUNT         5723 a         5263 c         5879 g         5562 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
      541 cgccatcaaa agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa
      601 cgcgctatta ctgggatatt cgtttagcgg ggctaacagt attcatagca gcgcagacag
      661 tgattctggc gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag
      721 attacgcaac aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct
      781 cagcagctat ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga
      841 ttatactgca ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga
      901 tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa
      961 aagcaatgcc cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc
     1021 gcctggtgct tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt
     1081 tgaaatcaaa gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc
     1141 attactccag cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa
     1201 tagtaatgaa cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg
     1261 gggaacgaca tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg
     1321 tctgggattt aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag
     1381 tacgctggca gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac
     1441 gctcaaccaa ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt
     1501 ctacaccctt tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga
     1561 tgatgaagat acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa
     1621 actgcaggtc aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag
     1681 ccagcaaact tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac
     1741 gcaaattaaa gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc
     1801 tgatgctgat caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag
     1861 aagtaatgat agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat
     1921 cgataacgaa gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg
     1981 taacctgagc tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag
     2041 cgccagcatg gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga
     2101 taacggcagt caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca
     2161 gggcattacc ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc
     2221 agagaatact cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt
     2281 tgtcccctat gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa
     2341 acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt
     2401 tctggcggag ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg
     2461 tataccgctg cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat
     2521 tattgatgat gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt
     2581 acgctggggc gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca
     2641 aattaactct gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc
     2701 gtgttattac tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct
     2761 gcttatatcg ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc
     2821 gttaaaactc tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag
     2881 tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt
     2941 ggccgatggg cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa
     3001 attaatgact atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg
     3061 gttcctttcg aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc
     3121 ggtcaacgtt taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata
     3181 ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa
     3241 tgctacaaca tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat
     3301 tacctgtctg gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc
     3361 gaggttgatc tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt
     3421 ggggccagaa cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta
     3481 gttaacgtca atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag
     3541 acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct
     3601 gctggtggga cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg
     3661 tctatccagt cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa
     3721 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg
     3781 cgttattttg ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt
     3841 cagtatgcat atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc
     3901 attacaaaat attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt
     3961 aagcactgct gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc
     4021 ggacgtcctt tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga
     4081 cacaggcgga agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc
     4141 agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca
     4201 cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga
     4261 cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc
     4321 tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg
     4381 gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa
     4441 atcaatgtgc agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg
     4501 gagaaatctt tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc
     4561 accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg
     4621 gatgcagcca gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt
     4681 aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg
     4741 caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt
     4801 gtacttcatt atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc
     4861 gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt
     4921 tccggcagac agagaatcga tatctatttt actgactaat accagtcagg aatcttggct
     4981 tattaacagc aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc
     5041 agcactatta ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt
     5101 gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt
     5161 aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg
     5221 ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta
     5281 tcaacaatta cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta
     5341 ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt
     5401 gccgcctaaa agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc
     5461 ctggcggacg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg
     5521 aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga
     5581 actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac
     5641 cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt
     5701 cagcaattac gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct
     5761 gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt
     5821 cttgataaag acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag
     5881 atcggtaccg tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg
     5941 gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc
     6001 gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat
     6061 aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat
     6121 gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg
     6181 ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat
     6241 ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct
     6301 gaagacgaat tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt
     6361 gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc
     6421 ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca
     6481 ctagaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct
     6541 gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt
     6601 aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct
     6661 ctttataaga taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg
     6721 cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg
     6781 tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact
     6841 ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc
     6901 tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct
     6961 tttgtgttga tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt
     7021 tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca
     7081 aaccgcaaat gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc
     7141 cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg
     7201 ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg
     7261 gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg
     7321 tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct
     7381 tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg
     7441 gcgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag
     7501 aataagggat acgaattccc tgattttcta attgctccag caacacttgt tggttatttc
     7561 cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt
     7621 tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt
     7681 ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat
     7741 cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg
     7801 ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt
     7861 taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg
     7921 ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac
     7981 aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag
     8041 caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc
     8101 agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg
     8161 caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat
     8221 cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca
     8281 caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt
     8341 caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat
     8401 gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg
     8461 ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta
     8521 gctataatgc gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt
     8581 tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct
     8641 ctccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag
     8701 tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac
     8761 tgaactggaa aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa
     8821 gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat
     8881 gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc
     8941 gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt
     9001 gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga
     9061 tgctttcaca cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg
     9121 cgttaacgtc tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg
     9181 tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc
     9241 agccgctatt gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg
     9301 tggttccggt acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt
     9361 gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga
     9421 agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt
     9481 ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc
     9541 ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct
     9601 gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag
     9661 cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg
     9721 tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg
     9781 tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca
     9841 cgttgccgaa agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg
     9901 cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta
     9961 ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg
    10021 ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg
    10081 tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt
    10141 gctgaaaacc cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg
    10201 cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct
    10261 cgatactggt ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa
    10321 aggcaaagat ttcctcaacc tgttttctta taccggcagc gctaccgtgc atgcgggatt
    10381 aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga
    10441 acgcaacctg cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg
    10501 cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac
    10561 cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct
    10621 gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa
    10681 acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat
    10741 tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct
    10801 gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg
    10861 tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt
    10921 gtttgtctgg tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt
    10981 gaacaagggc tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg
    11041 caacaggatc cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt
    11101 gaagaacaag cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac
    11161 ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac
    11221 ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac
    11281 gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg
    11341 gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc
    11401 atcgactggc tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac
    11461 gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg
    11521 gtgacctatc cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg
    11581 gaagaactac aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc
    11641 caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg
    11701 cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag
    11761 gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt
    11821 aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt
    11881 ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg
    11941 gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc
    12001 gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg
    12061 atggttaatg gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg
    12121 aaacgggcga tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg
    12181 gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt
    12241 gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg
    12301 ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa
    12361 ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa
    12421 caatatgtgg cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa
    12481 gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag
    12541 cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg
    12601 gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg
    12661 gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta
    12721 aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa
    12781 ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa
    12841 atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg
    12901 tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca
    12961 tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc
    13021 gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat
    13081 atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt
    13141 tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg
    13201 ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa
    13261 gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc
    13321 gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc
    13381 agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat
    13441 cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca
    13501 ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct
    13561 gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc
    13621 ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt
    13681 ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg
    13741 gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt
    13801 attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg
    13861 catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc
    13921 cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga
    13981 ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg
    14041 acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca
    14101 gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt
    14161 gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt
    14221 tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga
    14281 aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga
    14341 aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg
    14401 tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg
    14461 cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg
    14521 cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc
    14581 tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg
    14641 agatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac
    14701 gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac
    14761 cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat
    14821 gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc
    14881 ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga
    14941 tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa
    15001 agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg
    15061 taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta
    15121 ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa
    15181 tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa
    15241 aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc
    15301 tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg
    15361 cttggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt
    15421 gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa
    15481 cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt
    15541 gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc
    15601 tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg
    15661 agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc
    15721 gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg
    15781 attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag
    15841 ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg
    15901 gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt
    15961 gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg
    16021 cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag
    16081 cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat
    16141 gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa
    16201 cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg
    16261 ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct
    16321 taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg
    16381 tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt
    16441 gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg
    16501 gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca
    16561 gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa
    16621 cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg
    16681 agtcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg
    16741 gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac
    16801 tctgtctgat gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct
    16861 ggcggtatag atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag
    16921 acgacgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag
    16981 tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc
    17041 cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg
    17101 cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag
    17161 ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg
    17221 gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc
    17281 gtgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc
    17341 tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc
    17401 gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct
    17461 tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct
    17521 tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc
    17581 cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg
    17641 cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa
    17701 atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac
    17761 tgatcgactg aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt
    17821 atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc
    17881 tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg
    17941 aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt
    18001 tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg
    18061 ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg
    18121 cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca
    18181 gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag
    18241 aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg
    18301 ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc
    18361 caagcatccg ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga
    18421 caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa
    18481 atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg
    18541 cctaccagaa tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc
    18601 gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc
    18661 cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc
    18721 tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag
    18781 agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca
    18841 tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc
    18901 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac
    18961 tcagactctt ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac
    19021 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
    19081 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
    19141 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
    19201 ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt
    19261 taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa
    19321 gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc
    19381 ggttggaaat ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac
    19441 atagaagcca gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc
    19501 gtgctggtaa atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag
    19561 cagaccattc gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa
    19621 aaatcgatcg atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc
    19681 ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa
    19741 tacgcgaata aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa
    19801 ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt
    19861 ctaccagacg ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc
    19921 gatctctacg cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt
    19981 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc
    20041 tgccgggata cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag
    20101 accctggttg taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    20161 gtctttcgga tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    20221 cggtttcagg gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt
    20281 ggtctgtact tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa
    20341 acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc
    20401 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    20461 gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc
    20521 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    20581 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    20641 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    20701 catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    20761 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    20821 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    20881 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    20941 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    21001 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    21061 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    21121 agtcttgtat aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg
    21181 gaaaccgctt cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg
    21241 gcgacaaaaa aagttctagg attaatccta aatttactta atgatacaaa ttagagtgaa
    21301 tttttagccc ggaaagttgt ctcgttgtgt gagaggatgc gcttaccgga cgcataataa
    21361 accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca
    21421 aatcatctgt caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca
    21481 ttgactccac tgtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta
    21541 gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc
    21601 agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg
    21661 gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga
    21721 cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag
    21781 tgtacataat caatccagcc cctgtgagtt actgtatgga tgtgcagtac atccagtgac
    21841 aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa
    21901 aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg
    21961 ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc
    22021 tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga
    22081 tcattgtttg gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat
    22141 ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct
    22201 gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat
    22261 gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca
    22321 gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg
    22381 tcttttcatc tggaagcgat tctcggggag gtgggtagac tggcccc
//
LOCUS       DAJTUM010000062        22281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTUM010000062 DAJTUM010000000
VERSION     DAJTUM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1028)
                     /locus_tag="OO471_002577"
     CDS             complement(<1..1028)
                     /locus_tag="OO471_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243796.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OO471_002578"
     CDS             complement(1130..2110)
                     /locus_tag="OO471_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6243797.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OO471_002579"
     CDS             complement(2118..2828)
                     /locus_tag="OO471_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6243798.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO471_002580"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO471_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6243799.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OO471_002581"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OO471_002581"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6243800.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OO471_002582"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OO471_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6243801.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OO471_002583"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OO471_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6243802.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OO471_002584"
     CDS             9405..9515
                     /locus_tag="OO471_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6243803.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO471_002585"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO471_002585"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6243804.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO471_002586"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO471_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6243805.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OO471_002587"
     CDS             12397..13029
                     /locus_tag="OO471_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6243806.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO471_002588"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO471_002588"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6243807.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OO471_002589"
     CDS             16561..17493
                     /locus_tag="OO471_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6243808.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OO471_002590"
     CDS             complement(17534..18964)
                     /locus_tag="OO471_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6243809.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO471_002591"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO471_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6243810.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO471_002592"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO471_002592"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6243811.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OO471_002593"
     CDS             complement(20825..21094)
                     /locus_tag="OO471_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6243812.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OO471_002594"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OO471_002594"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6243813.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OO471_002595"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OO471_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6243814.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5513 a         5584 c         5333 g         5851 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJTUM010000063        22124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTUM010000063 DAJTUM010000000
VERSION     DAJTUM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            382..1008
                     /locus_tag="OO471_002596"
     CDS             382..1008
                     /locus_tag="OO471_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6243815.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1029..3130
                     /locus_tag="OO471_002597"
                     /pseudo
     CDS             1029..3130
                     /locus_tag="OO471_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3143..3781
                     /locus_tag="OO471_002598"
     CDS             3143..3781
                     /locus_tag="OO471_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6243816.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3845..4513
                     /locus_tag="OO471_002599"
     CDS             3845..4513
                     /locus_tag="OO471_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6243817.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4510..5295
                     /locus_tag="OO471_002600"
     CDS             4510..5295
                     /locus_tag="OO471_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6243818.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5299..6111
                     /locus_tag="OO471_002601"
     CDS             5299..6111
                     /locus_tag="OO471_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243819.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6123..7545)
                     /locus_tag="OO471_002602"
                     /pseudo
     CDS             complement(6123..7545)
                     /locus_tag="OO471_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7671..7976)
                     /locus_tag="OO471_002603"
     CDS             complement(7671..7976)
                     /locus_tag="OO471_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6243820.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8084..8160)
                     /locus_tag="OO471_002604"
     tRNA            complement(8084..8160)
                     /locus_tag="OO471_002604"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8124..8126),aa:Val,seq:gac)
     gene            complement(8165..8241)
                     /locus_tag="OO471_002605"
     tRNA            complement(8165..8241)
                     /locus_tag="OO471_002605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8205..8207),aa:Val,seq:gac)
     gene            8549..9805
                     /locus_tag="OO471_002606"
     CDS             8549..9805
                     /locus_tag="OO471_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6243821.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9846..11219)
                     /gene="mdtK"
                     /locus_tag="OO471_002607"
     CDS             complement(9846..11219)
                     /gene="mdtK"
                     /locus_tag="OO471_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6243822.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11434..12075
                     /gene="ribE"
                     /locus_tag="OO471_002608"
     CDS             11434..12075
                     /gene="ribE"
                     /locus_tag="OO471_002608"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6243823.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12115..13263)
                     /gene="cfa"
                     /locus_tag="OO471_002609"
     CDS             complement(12115..13263)
                     /gene="cfa"
                     /locus_tag="OO471_002609"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6243824.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13554..14765)
                     /locus_tag="OO471_002610"
     CDS             complement(13554..14765)
                     /locus_tag="OO471_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6243825.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            14878..15810
                     /locus_tag="OO471_002611"
     CDS             14878..15810
                     /locus_tag="OO471_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6243826.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(15807..16832)
                     /gene="purR"
                     /locus_tag="OO471_002612"
     CDS             complement(15807..16832)
                     /gene="purR"
                     /locus_tag="OO471_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6243827.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17131..17220
                     /gene="ynhF"
                     /locus_tag="OO471_002613"
     CDS             17131..17220
                     /gene="ynhF"
                     /locus_tag="OO471_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6243828.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17386..18555
                     /locus_tag="OO471_002614"
     CDS             17386..18555
                     /locus_tag="OO471_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243829.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18701..19282)
                     /gene="sodB"
                     /locus_tag="OO471_002615"
     CDS             complement(18701..19282)
                     /gene="sodB"
                     /locus_tag="OO471_002615"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6243830.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19410..20237)
                     /gene="mepH"
                     /locus_tag="OO471_002616"
     CDS             complement(19410..20237)
                     /gene="mepH"
                     /locus_tag="OO471_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6243831.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20572..20919
                     /gene="grxD"
                     /locus_tag="OO471_002617"
     CDS             20572..20919
                     /gene="grxD"
                     /locus_tag="OO471_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6243832.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20958..21458)
                     /gene="tnpA"
                     /locus_tag="OO471_002618"
     CDS             complement(20958..21458)
                     /gene="tnpA"
                     /locus_tag="OO471_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6243833.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21466..>22124
                     /locus_tag="OO471_002619"
     CDS             21466..>22124
                     /locus_tag="OO471_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6243834.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5714 a         5162 c         5726 g         5522 t
ORIGIN      
        1 acaactgctg aaattatacg aatttcggta tgtattttaa tttatagcga taatgttatt
       61 tttattttct gcaatgaacg tcacaaatga ttgtcgctta agttaaattt atttcaaatg
      121 ataataaata tcagttcgct tataatttct tgctttgtaa ttttaattga taacgatcaa
      181 tgttaagggc tcagaataat cacattattt tttctcctaa ccataaagga ttagtttatc
      241 ggcgttaaat cttttctcat acactatcgg gttagggaaa atatctggtg ttggggacag
      301 agtcagcagc gtattagttg aatgcttaat atcaggctgc tgaattgtca gcactgtgtt
      361 tgcgaagagg atgcgaaata aatgaacccg gttgatcgtc cactattaga tattggctta
      421 acgcgattag aattcttgcg tatatcagga aaaggccttg ctggtctaac aattgcccct
      481 gcgttactgt cacttttggg ttgcaaacag gaagatattg atagcggcac agtagggttg
      541 ataaatacac ccaaaggggt gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc
      601 tgtgaaatct cttgtaccaa cttcaacgat ggctcagtag ggacattctt ctcccgtatc
      661 aaaatccatc gcaattattt ctttggcgac aacggggttg gctctggcgg cggcctgtat
      721 ggcgatctca actataccgc ggacacctgc cgtcaatgca aagagccgca atgcatgaac
      781 gtctgcccga ttggtgcgat tacctggcag cagaaagaag gctgtattac cgtcgatcat
      841 aaacgttgta ttggctgtag cgcctgtact acggcatgcc cgtggatgat ggccaccgta
      901 aataccgaaa gtaaaaaatc ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc
      961 ccaacagggg cgttaaaaat tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg
     1021 aaaaaatcat ggctaacggt tggacaggta atatattaag agtcaatctc acgacaggaa
     1081 atattaccct cgaagattcc agtaagttta aaagttttgt cggtggcatg ggcttcggct
     1141 acaaaattat gtatgacgaa gtaccgctag gcacgaaacc tttcgatgaa gcgaataaat
     1201 tagtctttgc taccggccca ttacctggat ctggtgcccc ctgtagttct cgcgtaaata
     1261 tcacctcact ttctactttt accaaaggaa atttagtcgc gatgcccata tgggtggctt
     1321 ttttgcagcg caaatgaaat tcgctggata cgacgtcatt attatcgaag ggaaggcgaa
     1381 atcaccggta tggctgaaga ttaaagatga caaagttagc ctggaaaaag ccgatttctt
     1441 atggggaaaa gggacgcgcg caacgacgga agaaatttgt cgattgacca gcccggaaac
     1501 ctgtgtggcg gctattggtc aggctgggga aaaccttatt cctctctctg gcatgttgaa
     1561 tagccgtaac cacagcggcg gtgcgggaac tggcgcaata atgggttcga aaaacctgaa
     1621 agcgattgcg gttgaaggga cgaaaggggt caacattgcc gatcgtcagg agatgaagcg
     1681 tctcaatgat tacatgatga ctgaacttat tggtgcgaat aacaaccatg tcgtgccaag
     1741 tacgccacaa tcgtgggcag agtattcaga tcccaagtca cgctggacag cacgtaaagg
     1801 gctgttttgg ggcgcggctg aaggtggtcc gattgaaacg ggtgaaattc cgccaggcaa
     1861 tcagaatacg gtcggctttc gtacctataa atccgttttt gacttaggac cggcggcaga
     1921 gaaatacaca gtaaaaatga gcggctgcca ctcttgcccg atccgttgta tgacccaaat
     1981 gaatattcct cgggtgaaag agtttggcgt gcccagcaca ggtggtaaca cttgtgtagc
     2041 aaactttgtc cataccacca tcttcccgaa cgggccgaaa gattttgaag ataaagacga
     2101 tggtcgtgtg attggtaacc tggttggtct gaatttgttc gatgactacg gcctatggtg
     2161 taactacggg cagttgcatc gcgactttac ttattgttac agcaaaggtg tgttcaagcg
     2221 tgttctgcca gctgaagagt atgcagaaat tcgctgggat caactggaag cgggtgacgt
     2281 taacttcatt aaagattttt actaccgtct ggcgcatcgt gtgggtgagc tgagtcacct
     2341 ggctgatggt tcatatgcca tcgcagaacg ctggaatttg ggtaaagagt actggggcta
     2401 cgcgaaaaat aaactctggt cgccgtttgg ctatccggtt caccatgcca atgaagcgtc
     2461 agcgcaggtc ggttccattg ttaagtgtat gttcaaccgt gactgcatga cgcataccca
     2521 tatcaacttt attggttccg gcttgccatt gaaactgcaa cgtgaagtgg cgaaagaact
     2581 ttttggttct gaagatgctt acgatgaaac caaaaactac acgccaatca atgacgcaaa
     2641 aattaaatat gcaaagtggt cgctgttgcg ggtctgtttg cataacgccg tcactctgtg
     2701 caactgggtc tggccaatga ccgtttcgcc gctgaaaagc cgtaattacc ggggcgatct
     2761 ggcgcttgaa gccaaattct tcaaagcgat caccggcgaa gagatgactc aggaaaaatt
     2821 agatttagct gcagagcgta tctttacgtt gcatcgtgcc tacacggtaa aaccgatgca
     2881 aaccaaagat atgcgtaatg aacacgatct tatctgttcc tgggttttcg acaaggatcc
     2941 gcagatcccg gtctttactg aaggtactga caaaatggat cgtgacgata tgcatgcttc
     3001 gctcacgatg ttctacaaag aaatgggctg ggacccacag cttggttgtc caacccgcga
     3061 aacattgcag cgtctggggc tggaagatat tgccgccgat ctggcagcac acaatctact
     3121 gcctgcgtaa ggaatgggta agatgaacca tcaggatgaa ttgccgttag cgaaagtcag
     3181 cgaagttgac gaggcgaagc ggcaatggtt gcaggggatg cgtcaccccg ttgatacggt
     3241 aaccgaacca gaacctgctg aaatcctggc tgagttcatt cgccagcatt ctgcggctgg
     3301 gcagctggtg gcgcgcaccg tgtttctctc gccaccctat tcggttgcag aagaggaact
     3361 gtcagttttg ctggaaagca ttaagcagaa cggagactac gcggatatcg cttgtatgac
     3421 gggaagtcag gatgattact attactcaac gcaggccatg agtgaaaact acgcggcgat
     3481 gtctttgcaa gttgtggagc aggacatttg tcgcgccatt gcacacgccg ttcgttttga
     3541 gtgccagacg tatcctcgtc cttacaaagt cgccatgctg atgcaagcgc catattactt
     3601 ccaggaagcg cagattgaag ccgcgatcgc cgcgatggat gttgcacctg aatatgccga
     3661 tatacggcag gtggagtcat cgacggcggt gttgtacttg tttagcgagc gatttatgac
     3721 ctatggcaag gcttatggct tatgcgaatg gttcgaggta gagcagtttc agaacccctg
     3781 aaatttcagg gagatgtcat ggatagggtg gacagttgca gccactgcat taggggataa
     3841 tcagatgtcc ttcactcgac gcaaatttgt tctggggatg ggaacagtaa tcttttttac
     3901 tggttctgcg tcttctctgt tagcgaacac gaggcaagaa aaggaagttc ggtatgccat
     3961 gattcatgac gagtcacggt gcaatggctg taatatttgc gcccgcgcat gccgtaaaac
     4021 taaccacgtc cccgctcagg gaagccgttt atcgatagca cacattccag tcaccgataa
     4081 cgacaacgaa acgcagtatc acttttttcg ccagtcatgt cagcactgcg aagatgctcc
     4141 gtgcatcgac gtatgcccga cgggggcatc gtggcgtgat gagcagggga tcgtgcgggt
     4201 agaaaaatcg cagtgcatcg gctgcagtta ttgcattggc gcttgtccgt acctggttcg
     4261 ctaccttaac cctgtcacta aagtggcaga taaatgtgat ttctgcgctg agtcccgact
     4321 ggcaaaaggc tttccgccca tttgcgtcag cgcctgtccg gaacacgcat tgatctttgg
     4381 tcgcgaagat agcccagaaa ttcaggcgtg gttgcaggat aataaatact atcaatatca
     4441 gctacctggt gccggaaaac cgcatctgta tcgccggttc ggtcaacatt tgattaaaaa
     4501 ggaaaatgta tgaacccgtc gcaacatgct gaacagtttc agagccagtt agcgaactat
     4561 gtgccacagt tcactcccga attttggccg gtgtggttga tcattgccgg agtattgctg
     4621 gttgggatgt ggttggtgct ggggctgcac gccttgcttc gtgctcgtgg cgtgaagaaa
     4681 tcagccaccg atcatggtga gaaggtttat ctttacagca aagcggtcag attatggcac
     4741 tggtcgaatg cgttactctt tgtattgttg ctggcaagtg ggctgataaa tcactttgcg
     4801 atggtgggcg caactgcggt taaaagtctg gttgcggtgc atgaagtttg cggatttttg
     4861 ttactggcat gctggctcgg ctttgtgctg atcaatgccg ttggggataa tggtcaccac
     4921 tatcgcattc gtcgtcaggg gtggctggaa cgagcggcaa aacaaacgcg attttatttg
     4981 tttggcatta tgcaggggga agaacatcct ttcccggcaa caacacagtc taaatttaat
     5041 cccttacagc aggtcgccta tgttggtgtc atgtatggat tgctgccgtt gttactattg
     5101 acggggctgt tgtgtctcta tccgcaagcc gtgggagatg tgtttcctgg cgtaagatac
     5161 tggttattgc aggcgcattt tgctctggca tttataagcc tcttttttat cttcggtcat
     5221 ctttatcttt gcaccacggg gcgtacgcca cacgaaacct ttaaaagcat ggtcgatggc
     5281 tatcaccggc actaacgaat gatcattaca cgagcagacc tgcgagaatg gcgtatcgga
     5341 gcggtgatgt atcgctggtt cctgcgccat ttcccacgcg gcggtagtta tgccgatatt
     5401 cgtcatgcac tgattgagga aggatatacg gactgggcgg agagtctggt tgagtatgcg
     5461 tggaaaaaat ggctggcgga tgaaaacttc gcgcatcagg aagtttcctc tatgcaaaaa
     5521 ctggccacag accccggaga gataccattt tgcagtcagt ttgcccgcag cgacgatcat
     5581 gctcgcatcg gctgttgtga ggataacgcc cgaatcgcaa ccgctgggta tgccgcgcaa
     5641 attgccagta tgggttatag cgtgcggatt ggaagtgtcg gctttaacag tcacattggc
     5701 agctccggtg aacgcgcacg ggttgctgta accggtaatt cttcgcggat aagcagcgct
     5761 ggagatagta gtcggattgc gaataccgga atgcgagtac gcgtttgtac attgggtgaa
     5821 cggtgtcatg tcgccagtaa tggtgacctg gtgcaaatcg ccagctttgg cgcaaacgcc
     5881 agaattgcca acagcggcga caatgtacac atcattgcca gcggtgaaaa ttcaacggta
     5941 gtgagcacgg gcgtggtcga ctcaattatt ctcgggctag ggggaagcgc cgcactggca
     6001 tatcatgacg gcgaaagagt tcgcttcgcc gtcgccattg aaggtgaaaa caatattcga
     6061 accggagtcc gctatcgtct taatgaacag catcagtttg ttgagtgctg atagctttac
     6121 ctttaatcaa acgaaagacg ccgacgagca cgggatgcag gctttacgac cgcccgagcc
     6181 atcgcctcac caatttctgc acatgccgta agtccctgaa cgaaaggctg gtcgtgcatc
     6241 aaccagggca acgcgccgaa cgctacgcgt ccacgaatat cttcgggttg ctgtaacgta
     6301 taatcttcgc caacatcagg gatttcagat ccccgcttca cgtaaggcca gtagtcgacg
     6361 aatagactct gaagggattg ccgcataatt atcgatgaaa accctggcaa ggcctttact
     6421 gaaccgttta tggtcgtgtt cattcagatg aggaacaatt tcctgtacgg cttcatgcag
     6481 gcgcagaatg acataacgcc agggaacagt atgtttttct cgtttattgc gttcgacttc
     6541 ctggagattt ttttctgccc agtcaaatgg gtcgcgttgt ttgcgctcgg caaaccaggc
     6601 ttgagcaaag gaatcgacat tcaggctctc taaggctatg cgttggctcc agtctggatc
     6661 agcaaacttg atttcctcta ctatcaatct aaatacccga tccaaaaggc cctcttcacc
     6721 tttttgaatc tcagcattta atgcctgatc ggtgacgatg tgtaagggct cgtagggaat
     6781 agggcaatag aaatcggctt cgggtaaaat acccgtgcgc gacatcaacg taatatttag
     6841 cttttcactt gcgttatcgc ggtgaaaaac gacgtgttgt ttatcatctt caatgaacga
     6901 accatgctga atagccactg ccattgccgc atccagtccg ctcaaggatg ttcccataat
     6961 acccacgtta cacgcatcga cctttgcttc catcaggcct gaccacgggc tgggaaaata
     7021 cgttctggtt gcttcttctt catcaggcca gacgtgaccc gtggcaatca ccgctaaatc
     7081 aaacgtctcg ctgggtaaat cctgatttgt agcgagcatg acgccagcat ttgtaatttg
     7141 cagatcggta acctggcatg attcataaac agccactgca aatttttgct gtcgtgcctg
     7201 gtctactaat cgtaaaaatt gatcgcggaa atattcgccc agcagaattc gcggtaaaaa
     7261 ctgacgatcg tgcaaggttt cttttttaac gccataacgc tggagatggc tggcttcttg
     7321 cttttgtagc cattcgagat acgtacaatt aatcggcggt atttcaatac tggcaatatt
     7381 tgccagcatc atttttgagt tttcctcatc actgtatggc atcccgacac cggcctcgtc
     7441 agcctgctcg aagatagaaa tagaaagtgg agtttgttgc tgtagaagcg agaataaggt
     7501 gtagatcccc gtaggcccgg cacccacaat agcaattttt ttcattgtta cttacctgta
     7561 cgcattcttt cgaatttagt gttatcagat aagtttggtt gagattttta gaatttgaga
     7621 aatttcagac agggatgaaa agggcgctaa ccgcccttga ttaaattctg tcaggcgagt
     7681 tttgcctgat tgatttgact aagtggctca ttgacatcga aaactttggc gacaacttcc
     7741 tcaacgccga gatttttcag tcgggcagta tgtttttcca gataggcaag ggcgcttttt
     7801 tcatcagtga aaaggtagat cccaccggct tcgtggttct tttcactttc tgtccatacc
     7861 ttccacagaa aaccaggttc ctgattaatc gactcagcaa gtggtttaag ctgctcagcc
     7921 attgcgtcgc caaatgggcc attaaaagca aaatgaagtt gtaacaaagt tgccatagcg
     7981 attctccttc tgataacgca tacgaagaca gtgtaaatct tttaacgtat tggatacaga
     8041 gaagaaacag agggaaaaag gaaaagacca gattaagaaa atctggtgcg tccgagtgga
     8101 ctcgaaccac cgacccccac catgtcaagg tggtgctcta accaactgag ctacggacgc
     8161 aggatggtgc gttcaattgg actcgaacca acgaccccca ccatgtcaag gtggtgctct
     8221 aaccaactga gctatgaacg caacgttgta ggtgacaacg gggacgaata ttagcggcag
     8281 agtggaaagg tggcaagagg caaaacgtaa ttttctgcgc tatttcgacc gtttgcagag
     8341 cttttaagca aattggctat attttgttga tttgcaaggg cgatttttat tcaggatcgc
     8401 atttacatct gatacaaccc aataattcgt gtatcagagt ttaaacagag ttgtttgcca
     8461 ccatctcaaa tctgtcgtgg ataaatcctc cgtagccttt acacttaggt agcaataaag
     8521 gcacacagat gcagcaaagg aataaatcat gggatctgat gcgaaaaact tgatgagcga
     8581 cgggaatgtg caaattgtta agaccggcga ggtccttggc gcgacgcaac ttactgaagg
     8641 cgagttgatt gttgaagctg gcggaagagc cgaaaatacc gtggtcacgg gggctggctg
     8701 gttgaaagtg gcaaccggtg ggatcgccaa atgcacacag tacggtaaca atggcacgct
     8761 atcggtcagc gacggtgcca ttgccacaga tattgttcag tccgagggag gcgcaattag
     8821 tctctctacg ctcgctacgg ttaatggccg ccatcccgaa ggtgaattca gcgttgatca
     8881 gggttatgcc tgcggtttgt tgctggaaaa tggcggtaac ctgcgtgtac tggaaggaca
     8941 tcgcgcggaa aaaatcattc tcgatcaaga gggcggcctg ttggttaatg ggacaacctc
     9001 agcggtcgtg gtagatgaag gtggtgaatt gttggtgtat ccaagtggag aagccagcaa
     9061 ttgtgagatt aatcagggcg gcgtttttat gctggccggg aaagccagtg atacgttgct
     9121 tgctggtggc accatgaata atctcggtgg tgaagactct gacactattg ttgagaatgg
     9181 atccatttat cgtctgggga cggatggcct tcagctctac agttccggta agacgcaaaa
     9241 cctgtcggtg aatgtggggg ggcgggctga agtgcatgcc ggtacgctgg aaaatgcggt
     9301 aatacaaggt ggaacagtga tcctgttgtc acccaccagc gcggacgaaa attttgtcgt
     9361 agaggaagat cgcgcaccgg ttgaactgac cgggagtgtt gcattactgg acggcgcttc
     9421 aatgattatt ggttatggcg cagagctgca acaatcaacg attactgtac agcagggcgg
     9481 tgtgttgatt ctcgacggca gtacggtaaa aggtgacagc gtcactttca ttgttggtaa
     9541 catcaatctg aatggcggaa aactgtggct gatcactggt gcggcaacgc atgtgcaact
     9601 gaaagtgaaa cgcctgcgcg gagagggagc gatttgcctg caaaccagtg cgaaagaaat
     9661 ttcacctgac ttcatcaatg tgaaagggga agttaacggg gatatacgcg ttgagataac
     9721 agatgccagt cggcaaactc tgtgtaacgc tctgaaatta cagccagacg aagacgggat
     9781 tggcgcaacg ctccagcctg cgtaaaaaca caggcgcaac ccgtgaaggt tgcgccttgt
     9841 ctttattagc gggacgctcg ttgcagaatg atggctgacg gcatacgttg caggaaccgc
     9901 atacgcagca tcatcataat ggctgccgat gtcaggccga taataaagcc tatccagaag
     9961 cctgctggcc ccataggttc aacgaccaga tcggtcagtg ccagaatata gccgcttggc
    10021 aagcccagca cccagtaagc tgtaaaggtg atatagaaaa tggaacgcgt atctttataa
    10081 ccacgcaaaa tcccactgcc aatcacctgg attgagtcag aaatctgata taccgccgcc
    10141 agcaacatca aatgcgcagc cagcgttaca acctcgggat tgtcgttgta caacagggcg
    10201 atttgctccc gcagtgaaac cgtgaaaatg gccgtcaggg ttgccataca gacacccacc
    10261 ataagcccgg tccgcgcagc ggtttgcgca tccagcgttg agccctgacc cagacgataa
    10321 cctacgcgga tagttaccgc tgccgccagc gacattggaa gcacaaacat cagtgaacta
    10381 aagttcaggg caatctggtg tcctgcgaca tcaacaatac cgagcggaga cactaacaga
    10441 gccacgacgg caaacagtgt cacttcaaag aacagtgcca gcgcaatcgg caaaccgagt
    10501 tgaatcagtc gtttcataac cgcaggctcg ggcttagcgg tgcctttttc gttacgaata
    10561 tcgcgcatgg agcgggcgcg tttaatgtaa gaaaccatgg caaggaacat gacccaatac
    10621 accgccgcag tagccacgcc acaaccaacg ccaccgagct caggcatacc gaaatgacca
    10681 taaataaaga tatagttcac cgggatgttc accagcaggc cgataaagcc cattaccata
    10741 cccggcttgg tttttgccag accttcacat tggttacggg caacctggaa gaacagatat
    10801 cccggcgcgc cccacaacaa cgcacgcaga taacccacgg ctttgtccgc cagagccgga
    10861 tcgatgtttt ccatgtagcg gataatgtaa cctgcattcc acagcaccag cataatgaga
    10921 acggaaacaa aacctgccag ccagaaacct tgtcgcacct gatgcgcaat gcgctcacgt
    10981 cgaccggaac catttaattg cgcgataacc ggcgttaatg ccagcagcag tccgtgacca
    11041 aagaggatcg ccggaagcca gatagaagta ccgatagcga ccgccgccat gtcggtggca
    11101 ctatagccgc ccgccatcac ggtatcgaca aaacccatcg cagtttgggc gatttgcgcg
    11161 agaatcaccg ggattgctaa tgctaataac agacgcgctt cactgatata cttctgcacg
    11221 tgaacacctt ttatttgtag ttatatgaga gactaaaaag ccgcctgaat gggcagcaaa
    11281 aagaagaagc aggggaaatt cagtctattg tagcgaggat ttattaattc tccagtgaaa
    11341 aaatagccat tcttacccct tcactggtaa gggcgttttc cacctgctat tgtgctgaac
    11401 agaatgtctt aactgatttc aggagttgta agtatgttta cggggattgt acagggcacc
    11461 gcaaaactgg tgtcgattga cgagaaacca aattttcgta cgcatgtggt ggagttaccc
    11521 gaccacatgc tggacggcct ggaaaccggt gcttccgtgg cgcataacgg ttgctgcctg
    11581 accgtgacgg aaattaacgg caaccatgtc agttttgacc tgatgaaaga aacgttacgc
    11641 attaccaatc ttggcgattt aaaagtgggg gattgggtaa acgttgagcg tgcggcgaaa
    11701 ttcagtgatg aaattggcgg acacttaatg tcaggtcata ttatgaccac cgctgaagtg
    11761 gcgaaaatat taacctcgga aaataatcgc cagatctggt ttaaagtcca ggatagtcag
    11821 ttgatgaaat atatcctgta caaaggattt attggcatcg acggtattag cctgaccgtc
    11881 ggcgaagtca cgccaacgcg tttttgcgtc catttaattc cggaaacact ggaacgcacg
    11941 actcttggga agaaaaaact tggcgcacgc gtcaacattg aaatcgatcc acaaactcag
    12001 gcagtggtag atacggtaga acgtgtgctg gcggcacgag aaaatgccat gaatcaacca
    12061 ggtacagaag cctgatctga actcgccccc gaagaggggg cgatagaata gcctttagcg
    12121 agcgactcga aggccgtttt caacaccgcg tgagaacacc acctgccaga gctgaatatc
    12181 acgggcgcgg aaagcacctg cgcaggcatt cagataatag gtaaacatcc gtttaaagcg
    12241 ttcactgtag ttatccgcaa tttctggcca tgcggcgagg aatcgttcat accacgccat
    12301 caacgtagta tcgtaatcag caccgaagtt atgccagtct tccatcacaa agtggggttc
    12361 gctggattga gcaatctggc gtacagaggg caggcaaccg ttcggaaaaa tatatttatt
    12421 aatccaggga tcaacattca gatcggtttt tttcgaaccg atagtatgga gcaggaatat
    12481 accttccggt ttcaaattac gatccaccac cgcaaaatag gtatcgtaat ttttcggtcc
    12541 gacgtgctcg aacatcccca cagaaacaat acgatcaaac tggtcgttca ggtcacgata
    12601 atcttgcagc aaaatggtga catccaggcc tttacagcgt tcctgagcca ttttttgctg
    12661 ttcggcagaa atggtgacgc cgaccacgct tacgtcatag ttagatgcca tgtagtgcgc
    12721 tagtccgccc cagccgcagc caatatccag tacgcgcatc cctggtttta actgcaattt
    12781 ttcacaaatc attttgagct tcgcctgctg ggcagattcc agattatcgg catctttcca
    12841 gtaagcgcag gaatattgca tgaagggatc aagcatgcgg ctgaacaagt cattacccaa
    12901 atcgtaatgc tccttgccga ctatccaggc acgtttttta ctctgcagat tgaagagacg
    12961 agcgccggca atacgcagcg tgtctttgaa atgatggggg agttggttct cgagacctgc
    13021 gcgtaagact ttgctaaaaa acatatccag tcggtcacat tcccaccagc catccatata
    13081 actttcgcct aaccccaaag agccttcctg cagaacgcgt ttaaaaaaat cggggttttt
    13141 cacacgaata tccgccgggg cagaaccgtt aatggctata ccggcacggc taagtaattc
    13201 gttggcgata cggtaccagt tgtcatccgg tacactgact tcttctatac acgatgaact
    13261 catagtttct ccatcactgg tcggtgatca gaaccttaaa acagcgtaga cgctttttgg
    13321 gctttgtgag aaatctcgca gaaaaaaccg cgagtctata atccgacgta gaacagaggg
    13381 aaaggaggaa cccttgccga aatgaccatc catagtgagt cgggaacgat cctgtccccg
    13441 taaaaacata atatttatcg tagctttaac aactcgaaat tagtataggc cgctaatttc
    13501 aatcatacaa caaaattttg ttagcagcct aagtataagt atatcgatat agatcagtgt
    13561 gattcgctat gagcgacttc ggcattgcca tgattcgggc agtcaacttc ttcacaacgt
    13621 tgcatcatgt aacccagcgc gaccagcact actgttgata acatcacgct ggtggtggtg
    13681 agcaatggcg tgctgatact aataagccag gaaactacca gacttgcgag gaagcacaga
    13741 cccagttgca gagtgttctg caacgctgcg gcgcgaccgg ttgcatgtgg gaagggacgc
    13801 agcgcctggg cgacaacaat agggtagatc gcgccattgg caatcgccat cacacagaat
    13861 gggatcagga tttcgaccag cgacacatgg ctaatgaagc ccgcagccca ggtcgcaatg
    13921 acgctgacag caaacagcac cagcaaccac ggtaataact gcttgccttg ccatttctgc
    13981 agtgcggcgc gacagccata accaccaatc agaaacgcga tagtttgcgg gacataactt
    14041 aaaccaataa ctgccgggct gtagcccatt tcactaagga tgaacggtga accggtcagc
    14101 catgcaaaaa aactggctga acaggctgcg tatatcagca cgttgccgcg ataggtttta
    14161 gaacgtagca ggtcggtaaa ggtcagacca tcctgactat tgttacgggc cttcgtcgtg
    14221 ggtttgagcc agaaaatagg cagaatcagc accacggtaa tggcaaacag ggtggcgaaa
    14281 atcgcctgcc aggaaaaatg gaccagcagc cagcttccta acaggggagc cagtgccgga
    14341 gacagaccca ccagcggcat gatggtcgca aaaatacggt tgactttctg tgaaggataa
    14401 taatctgtca ctaatgcttg ccagataacc gccgcggcgc agacccccac agcctgtaca
    14461 aaacgcaata ccagcagcgt ggcggcgttt tctacccaca gcatccccag actacctaac
    14521 gcaaaaattg tcaggccgat taataacacc ggtttacgac cataacggtc ggagagtggc
    14581 ccccacagaa gctgggctgc ggcaaaaccg gcaaggaaca gactaaggct ggcactgaca
    14641 gcagacgcag gcgtttgcag gtcggcctgt atggcggcga aagcaggcag atacatatcg
    14701 gttgccagaa aaccgagtac gctcaaaccc gccagccaga ctaaaaatct tttcccaggt
    14761 tgcatgtata ttctctaaat ctctcaatta aacaccgccg caaagtgtag ggagtgaaat
    14821 tagtgttgtg aaacggtaat atttgatgac tggtttcaaa aattttgcag gcagactatg
    14881 tggtcagaat attcactcga agttgttgat gcggtagcgc gtaatggtag ttttagcgct
    14941 gcggcacagg agctgcatcg tgttccttct gcggtcagct ataccgtgcg tcagctggaa
    15001 gagtggctgg cggtaccgct ctttgaacga cggcaccgtg atgtggagct gaccgctgct
    15061 ggagcgtggt ttctcaaaga agggcgctct gttgtcaaaa aaatgcagat cacccgccag
    15121 caatgtcagc agatagcgaa cggctggcgc ggtcagttag ctatcgcagt ggataatatt
    15181 gtcaggccag aacgtacacg gcagatgatc gttgattttt atcgccattt tgatgatgtc
    15241 gaacttcttg tctttcagga agtgttcaac ggtgtctggg atgcgctttc cgacgggcgc
    15301 gtggaactgg caattggcgc aacacgcgcg attccggtag gcggtcgtta tgccttccgg
    15361 gatatgggga tgctaagctg gagttgcgtt gttgccagtc accacccgct ggcgttgatg
    15421 gatggcccgt tcagcgatga tacgttgcgc aactggccgt cgttggtgcg cgaagacacc
    15481 tcgcgaacgc tacctaaacg tattacctgg ttgctggata accaaaaaag acttgtcgta
    15541 cctgactggg aatcatcggc aacctgtatc tcggcaggat tatgcatagg gatggtgcca
    15601 acacatttcg ccaaaccgtg gcttaatgag gggaagtggg tagcacttga actggaaaac
    15661 cccttcccgg attcggcatg ttgtctgaca tggcagcaaa atgatatgtc gcctgcgtta
    15721 acctggctgc ttgagtatct cagcgatagt gagacgctga ataaggagtg gctgcgggag
    15781 ccggaagaga ctcccgcaac gggtgattaa cgacgatagt cgcggaacgg gccgtcagcc
    15841 acggagcggc gttcaatcaa acgcggatgc acttcaatgg actgcggttc ttcacgtttg
    15901 ttgacgatac gatccaacag catgttgaac gctgtttcac ccagcgaatc ttttggctga
    15961 tggatcgtgg tcagcgccgg cgtaaaatag cgcgcgttgc gcacgttatc ataaccgatc
    16021 agcgaaacat cctgcgggac gcgcaggccc atttcatcag cagcacaaag tgcgcccatt
    16081 gccatgatat cgccaccaca gaagacggca gtagggcgat gcggctgcga caggatttgc
    16141 tgcatggcgc gataaccgga ttcaggttca aagtcaccct gcacaatcca gctttccggc
    16201 accttgatca tcgcttcttc catcgccttc ataaaaccgg caaggcggcc tgcgccggtg
    16261 ttacgttcca gcgggccggg gatgacgccg atttcgcggt gaccgcgttc aatcagataa
    16321 cgcccggcca tgtagccgcc ttcgaacgcg ttatcaatga ccgcatcggt gaagtcagct
    16381 tttgcttcac cccagtccat caccaccatt gggatatggc gatactcttc cagcatcgcc
    16441 agcaacggct ctgggtactc agaacacatc accagcagac catcgacgcg tttttgcgcc
    16501 atcatcgaca gataagcccg ctgtttctca agattgttcc acgcattacc cagaatcagg
    16561 gtgtaacctt tctggaagca atttttttca actgcttcaa tgatctcggc aaaataggcc
    16621 gcttcgctgc tggtcgccag caaaccgata gacttggtgt ggttaacctt caggctacgc
    16681 gccaccgcgc taggggagta gtgtaattct ttaatcgctg cccacacggc gttgcgcgtt
    16741 tcttcagcga cgaaacgtgt tttgttgatc acgtgtgaca cagttgtagt ggaaacgttt
    16801 gctcgtttcg ctacatcttt tattgttgcc attccatttc actccagacc ctaacttcag
    16861 ctcctgcaaa atcgcaaggt aaacgtttgc ctttacacac cttacgcaca acaaaagtgt
    16921 tgcggtacgc cggaaaaacg acacggagcg tcaggaaggg gtcaatggcc agtacgcaaa
    16981 tagtgtaagc gtggaatttt ggccgatctt gacgaaaagg ggaagtggta aaaacactta
    17041 tcattataaa tgacgggaga ttttttcatc acagtgtgta aaaatgcgta atatcacttt
    17101 ttgtgtggta ataaaaaagg agaaaacttg atgagcaccg accttaaatt ttcactggta
    17161 acaacggtta tcgtcctcgg tttgatcgta gccgtgggtt tgactgccgc gctgcactga
    17221 tttcctctcg agggagcgcg ttctccctcg catctccttc ctgtttgtta gcaaaatggc
    17281 aaaagtgttt tctctttttg ttattttcat ttttttgtga ttgctgtcat gctttcgcca
    17341 ctttaatgaa tatcgcgtaa tgacgcgaag aggtggagat aaggcatgaa aattaactat
    17401 ccgttgctgg cgctggcgat tggcgcgttt ggtatcggga caacggagtt ctcgccaatg
    17461 ggcttgttgc ccgtcattgc gcgcggtgtg gatgtctcga ttcccgctgc cggaatgtta
    17521 atcagtgcct atgcagttgg cgtaatggtg ggcgcgccgc tgatgacgct tctactttct
    17581 catcgtgccc gccgcagtgc gttgattttc ctgatggcaa ttttcacgct aggcaacgtt
    17641 ctttccgcca tcgcgccgga ttatatgacc ctgatgcttt cacgtatttt gaccagcctg
    17701 aatcacggag cattttttgg tttgggttca gtcgtggccg caagcgtggt gccaaaacat
    17761 aaacaggcca gcgcagttgc cactatgttt atggggttaa ccctggcaaa tatcggtggc
    17821 gtgccggcgg cgacctggtt gggtgaaacc atcggctggc ggatgtcatt tctggcaacg
    17881 gcggggctgg gagtgatttc aatggtaagt ctgttcttct cattacctaa aggtggtaca
    17941 ggggcacgac ctgaagtgaa aaaagagctg gcggtattaa tgcgtccgca ggtgctgtct
    18001 gcattgctga cgacggtact gggagctggt gcaatgttta ccctctacac ctatatctct
    18061 ccggtactgc aaagtattac ccacgcaaca ccggtgttcg tcacggcaat gctggtgctg
    18121 attggtgtcg gattctctat cggtaactat ctcggcggca aactggcaga tcgttcagtt
    18181 aacggcacgt tgaaaggctt tttgttgctg ctgatggtga ttatgctggc aatcccgttc
    18241 ctggcccgca atgagttcgg cgcagctatt agcatggtgg tgtggggcgc agcaaccttt
    18301 gcggttgtac ctccgttaca gatgcgcgtg atgcatgtcg ccagtgaagc gccaggtctg
    18361 tcttcatcag tcaatattgg tgcctttaat cttggaaatg cgctgggagc agctgctggt
    18421 ggtgcggtaa tttccgctgg gctgggatac agctttgtgc cggtgacggg ggcgattgtc
    18481 gcgggactgg cattattgct ggtgtttatg tcagccagaa aacaacctga aacagtttgc
    18541 gttgctaaca gctaattaaa caaaaacgca gaaggataac cttctgcgtt acagttgctg
    18601 acaacgtgcg atttatcttg ccgaatgcgc tgttaacgcc tcatccgcgt tggcatggta
    18661 agcgaagcgc atcaggcaat gctgcatttg ccatcagtta ttatgcagcg agatttttcg
    18721 ctacgaattc ccagttcacc agcgcccaga agtgctccag atagccagga cgtgcattgc
    18781 gatagtcgat gtaataagcg tgttcccaga catcaacggt cagcagcgga gtcgcatcgg
    18841 tggtcagcgg agtacccgcg ttagaggttg aaacgatagc cagtttgcca tcgctgtttt
    18901 tcaccagcca ggtccagcca gaaccaaagt ttttgatcgc tgcatcagta aactgcgctt
    18961 tgaaatcggc aaagctgcca aaagatgcgg cgatagcttc agcgactttt ccagtcggtt
    19021 cgccaccggc gttcggtgcc aggcagttcc agtagaaagt atggttccag acctgagctg
    19081 cgttgttgaa tacgccacct tcagagctgc gaataatctc ttccagtgat ttaccttcaa
    19141 acgcggtacc tttaatcagg ttgttcaggt tagtgacata agtctgatga tgcttgccgt
    19201 agtgatactc gatagtttcc gcagaaatgt gcggtgccag agcatcttta gcatatggta
    19261 gtgcaggtaa ttcgaatgac attgctactc tcctttatta ttaatttgca tacgtacaat
    19321 agccttattg tgcgtatgga tagggtagca aattaaaagg tgagacaaaa gatgaactta
    19381 ccctgttgcc gtaacaacag ggtaaagttt tagcgaagtg tttttggggt cattacccga
    19441 cgagcgccaa catagtggcg ctgccagtag tcttcactga gagaagtgat ttgaatttcc
    19501 tgacctgtgc gcggtgactg aataaatttg ccgttgccga catacacgcc gacatgatcg
    19561 gctgtaccgc gtccctgagt acggaaaaag accaggtcgc cgtttttcag ctcactacgt
    19621 tcgataggtc ctgcatcacg caggtgatac atttcattcg ccgtacgcgg aatacgaatt
    19681 ttcaccaaat ctttataagc gtaataaacc aggccgctgc aatcaaaacc ggtacgcggt
    19741 gagctgccgc cccaacgata tggcttacca atttgctgca tcagtttatt cattgccact
    19801 ttcgtggctt tttgcacctt cgctttgtgc gcgtcggcaa gggttcctga cgcggcattt
    19861 ttgactttca cacaatgcga tttatagccc ttacgtttgg tacacttttc ggtaaagctg
    19921 gctgaggccg ttttattgac ggcgtgtttg ctgcgagttt tggcgttttt agaagcggta
    19981 atggaagatt ttttaacaga agaagcggtt ttgcttttgg tcatactttt actggcggct
    20041 tttttcgctg ttttctggct ggctttctgg gtctttttgg tggttgccgt gcttttcttt
    20101 ttatctgctt ttttggtgat atgagtggta gcagaactct ccctggcttg ctttgaagca
    20161 tgggccatag gcgttaaagg cagggtggta aaaagtaaag cacagagcgt gatcgaaata
    20221 cggtttatcc gcgccactga gcgtccctct gttaaggcag gtctatcatg cctgcgtctg
    20281 atttacaaaa cttgatgatt ctaatgcata gaagaagaga aagttaatag cttttattgt
    20341 taaaaaatac gtttcgttgg cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtt
    20401 ataaatacgc cagtttgccg cgcatcagag aagaaacgaa tatttgaaat gcaacaaata
    20461 gagcaaaggg ataaaatagc aaaagcgtga tgaaaatgtt attttccgat gccgcactga
    20521 cccgctacaa tgtcagatta ctgccgtaaa gaagtttgag gaagcaagac aatgagcacc
    20581 actatcgaaa aaatccaacg ccagattgct gaaaacccga tcctgctgta catgaaaggt
    20641 tcaccgaaac tgccgagctg cggtttctct gcccaggcag tccaggcgct tgccgcatgt
    20701 ggcgaacgtt ttgcctatgt tgatattctg cagaatccgg acattcgtgc ggaactgccg
    20761 aaatatgcta actggccgac cttcccgcaa ctgtgggttg acggcgagct ggtcggcggt
    20821 tgtgatatcg tgatcgaaat gtatcagcgt ggcgaactgc agcagctgat caaagaaact
    20881 gccgctaaat acaagtctga agagccggac gcggaataat ccgtcctgca atactgacgg
    20941 atgcgaacgg gcatccgtca gtcctttatc cggtgcgccg taaaacctcg tccttcaggg
    21001 cggggatata aggcgcggtt ttccacctga ccaggtgttt gctgttgttc aatgtactgg
    21061 cggatgacgg atattggcgc accttcgcag ctactggcaa aatagccagg actccacaaa
    21121 acgcctttgt agtaatacct gactgcaata tctggtcgat ctcgtcgcag taacctattc
    21181 gatatgcctt tgaggctgtt taccagactg gatatcgcca gtttgggagg atagttaatt
    21241 aacaaatgga cgtgatctgg ttcgccatcc atttcaacca gttcagctgc aaaatcagca
    21301 catacatttg aaaagtaagt gcgtagtttt tctgtcgtgt catggtcaaa aatctggcgt
    21361 cggtatttgg tgacaaagac caggtgaaca tgcatcagga aaacacaatg ccttccacgc
    21421 cggatatcgg tttctttttt cacagaccaa agtatcattt gacctgtgaa acgattacag
    21481 gcatttaaat tccagttaag acccggtggt caacaggagc gtgaaatgag gcgcttcgca
    21541 ggcgcatgtc gtttcgtttt caatcgtgct ctggcgcttc agaatgagaa tcatgaggcc
    21601 ggtaataaat acatccctta cgggaaaatg gcttcctggc tggttgagtg gaaaaacgcc
    21661 actgaaacgc aatggcttaa agattcgccc tcacagccat tgcaacagtc actgaaagac
    21721 cttgagcggg cttacaaaaa cttcttccag aagcgggctg cttttccccg attcaaaaag
    21781 cggggacaga atgctgcatt ccgctacccg caggacgtta agctcgatca ggaaaacagc
    21841 cgtatttttc tgccgaaact tggctggatg cgctaccgga atagccgtca ggtcacggga
    21901 gttgtgaaaa atgtcactgt cagccagtcc tgcggtacgt ggtacatcag tattcagaca
    21961 gaaagtgaag tatcaactcc ggttcaccct tcagcatcaa tgatcgggct ggatgctggc
    22021 gtggctaaac tcgccacgct gtcagatggc acagtctttg agcctgtaaa cagttttcaa
    22081 aaaaaccaga agacgctggc gagacttcag cgacagttaa gccg
//
LOCUS       DAJTUM010000064        21531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTUM010000064 DAJTUM010000000
VERSION     DAJTUM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO471_002620"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO471_002620"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6243835.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OO471_002621"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OO471_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6243836.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OO471_002622"
     CDS             complement(2650..3201)
                     /locus_tag="OO471_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6243837.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OO471_002623"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OO471_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6243838.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="OO471_002624"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="OO471_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO471_002625"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO471_002625"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6243839.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO471_002626"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO471_002626"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6243840.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO471_002627"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO471_002627"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6243841.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO471_002628"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO471_002628"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6243842.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO471_002629"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO471_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6243843.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="OO471_002630"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="OO471_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6243844.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="OO471_002631"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="OO471_002631"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6243845.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO471_002632"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO471_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6243846.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO471_002633"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO471_002633"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6243847.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO471_002634"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO471_002634"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6243848.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO471_002635"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO471_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6243849.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="OO471_002636"
     CDS             complement(18171..18566)
                     /locus_tag="OO471_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6243850.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO471_002637"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO471_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6243851.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="OO471_002638"
     CDS             19482..20075
                     /locus_tag="OO471_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6243852.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /gene="tabA"
                     /locus_tag="OO471_002639"
     CDS             20220..20672
                     /gene="tabA"
                     /locus_tag="OO471_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6243853.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21531
                     /locus_tag="OO471_002640"
     CDS             20795..>21531
                     /locus_tag="OO471_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243854.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5202 a         5326 c         5673 g         5330 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaggttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctgatggtt cgactaatcg
    21241 ttatgaaggg aagtcttttg aaagaaaaga tgatagttca ttacacctga ttactaacaa
    21301 ggttctggcg tgttaccaac gcgaagctaa caagaaaata gcgcgtctat taaataatca
    21361 tcagaagtta aataatatac aggagttaaa tgattcgcag gagttaaatg attcgcagga
    21421 gttaaataat tcacagaagt taaataattc gcaggagtta aataactcgc aggtgagttg
    21481 taaaggttca gttgattcta cgattacgga tttattagaa aaagcattga a
//
LOCUS       DAJTUM010000065        21496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTUM010000065 DAJTUM010000000
VERSION     DAJTUM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO471_002641"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO471_002641"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6243855.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO471_002642"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO471_002642"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6243856.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO471_002643"
     CDS             complement(3121..3420)
                     /locus_tag="OO471_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6243857.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO471_002644"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO471_002644"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6243858.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO471_002645"
     CDS             complement(4632..5069)
                     /locus_tag="OO471_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6243859.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO471_002646"
     CDS             complement(5115..5456)
                     /locus_tag="OO471_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243860.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO471_002647"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO471_002647"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6243861.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO471_002648"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO471_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO471_002649"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO471_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6243862.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO471_002650"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO471_002650"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6243863.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO471_002651"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO471_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6243864.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO471_002652"
     CDS             12687..13217
                     /locus_tag="OO471_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6243865.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO471_002653"
     CDS             13329..13865
                     /locus_tag="OO471_002653"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6243866.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO471_002654"
     CDS             complement(13973..14797)
                     /locus_tag="OO471_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6243867.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17146
                     /gene="malS"
                     /locus_tag="OO471_002655"
                     /pseudo
     CDS             15117..17146
                     /gene="malS"
                     /locus_tag="OO471_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="OO471_002656"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="OO471_002656"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6243868.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="OO471_002657"
     CDS             complement(18728..19201)
                     /locus_tag="OO471_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6243869.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPAACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OO471_002658"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OO471_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6243870.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20351..21343
                     /gene="yiaK"
                     /locus_tag="OO471_002659"
     CDS             20351..21343
                     /gene="yiaK"
                     /locus_tag="OO471_002659"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6243871.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21355..>21496
                     /locus_tag="OO471_002660"
     CDS             21355..>21496
                     /locus_tag="OO471_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6243872.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5399 a         5489 c         5277 g         5331 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgcgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggtgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccgga caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctgg ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gtaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctgctggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgttctc
    20881 ttacggcatg ttagaagtta accgcctggc aggccgtcag ctcccggtcg atggtggctt
    20941 tgatgatgag ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt
    21001 gccgatgggc tactggaaag gttctggcat gtcgattgtg ctggatatga tcgctactct
    21061 cctttccgac ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat
    21121 ttcacaaatt tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc
    21181 caagctgcaa cgtatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc
    21241 catccgctta cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggcat
    21301 cactgttgat gacagcgtgt gggccaaaat ccaggcgtta tgaggagata agtcatgatt
    21361 tttggacata tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc
    21421 gattttctgc gcgctaccaa cttcaacgtc ctggagccgg gcgttgtcga aatcgacggc
    21481 aagaatattt atgcgc
//
LOCUS       DAJTUM010000066        21280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTUM010000066 DAJTUM010000000
VERSION     DAJTUM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..448)
                     /locus_tag="OO471_002661"
     CDS             complement(<1..448)
                     /locus_tag="OO471_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6243873.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(503..1354)
                     /gene="manZ"
                     /locus_tag="OO471_002662"
     CDS             complement(503..1354)
                     /gene="manZ"
                     /locus_tag="OO471_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6243874.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1367..2167)
                     /gene="manY"
                     /locus_tag="OO471_002663"
     CDS             complement(1367..2167)
                     /gene="manY"
                     /locus_tag="OO471_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6243875.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2230..3201)
                     /gene="manX"
                     /locus_tag="OO471_002664"
     CDS             complement(2230..3201)
                     /gene="manX"
                     /locus_tag="OO471_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6243876.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3664..5214
                     /gene="yoaE"
                     /locus_tag="OO471_002665"
     CDS             3664..5214
                     /gene="yoaE"
                     /locus_tag="OO471_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6243877.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5218..6816)
                     /locus_tag="OO471_002666"
     CDS             complement(5218..6816)
                     /locus_tag="OO471_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6243878.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6947..8311)
                     /gene="sdaA"
                     /locus_tag="OO471_002667"
     CDS             complement(6947..8311)
                     /gene="sdaA"
                     /locus_tag="OO471_002667"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6243879.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8495..9073)
                     /locus_tag="OO471_002668"
     CDS             complement(8495..9073)
                     /locus_tag="OO471_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6243880.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9077..10438)
                     /gene="pabB"
                     /locus_tag="OO471_002669"
     CDS             complement(9077..10438)
                     /gene="pabB"
                     /locus_tag="OO471_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6243881.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10512..10691
                     /locus_tag="OO471_002670"
     CDS             10512..10691
                     /locus_tag="OO471_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6243882.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10811..11170)
                     /locus_tag="OO471_002671"
     CDS             complement(10811..11170)
                     /locus_tag="OO471_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6243883.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11532..11876)
                     /locus_tag="OO471_002672"
     CDS             complement(11532..11876)
                     /locus_tag="OO471_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6243884.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12008..13918
                     /locus_tag="OO471_002673"
     CDS             12008..13918
                     /locus_tag="OO471_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6243885.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13976..14671
                     /gene="tsaB"
                     /locus_tag="OO471_002674"
     CDS             13976..14671
                     /gene="tsaB"
                     /locus_tag="OO471_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6243886.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14711..15292
                     /gene="yeaY"
                     /locus_tag="OO471_002675"
     CDS             14711..15292
                     /gene="yeaY"
                     /locus_tag="OO471_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6243887.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15498..17183
                     /gene="fadD"
                     /locus_tag="OO471_002676"
     CDS             15498..17183
                     /gene="fadD"
                     /locus_tag="OO471_002676"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6243888.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17265..18380
                     /gene="rnd"
                     /locus_tag="OO471_002677"
     CDS             17265..18380
                     /gene="rnd"
                     /locus_tag="OO471_002677"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6243889.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18434..19399)
                     /gene="yeaX"
                     /locus_tag="OO471_002678"
     CDS             complement(18434..19399)
                     /gene="yeaX"
                     /locus_tag="OO471_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6243890.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19455..20072)
                     /gene="yeaW"
                     /locus_tag="OO471_002679"
                     /pseudo
     CDS             complement(19455..20072)
                     /gene="yeaW"
                     /locus_tag="OO471_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20414..20980)
                     /locus_tag="OO471_002680"
     CDS             complement(20414..20980)
                     /locus_tag="OO471_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6243891.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21192..>21280
                     /locus_tag="OO471_002681"
     CDS             21192..>21280
                     /locus_tag="OO471_002681"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6243892.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5267 a         5274 c         5466 g         5273 t
ORIGIN      
        1 atacgagaag cttataaatt ttttccagat cgtcgatatt tcgaacgcgg attaacagcc
       61 gacgctgttc taattgcatc accaacacgc catcttccga caagttcatc gctttgattc
      121 ggctatattc aatccagaca ttggcgaaga aaaaaccttt ttgtttaaag atgatcttcg
      181 gaatgcggat ccagaatata taaaaaccca tcagagccag tgcacttaat aaccatgttg
      241 ttattagcgc gccatgattc gtgacgttgt tatagataag aatgacaatc aatccgacga
      301 agataacgct atcgatgcga ccacgccgga gcaaaggaat tgccagcagg gtggggccgt
      361 tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc caggagtgcg gcgatgaaaa
      421 gaatcagtac caggtccgtg attgtcatta atcctccaga taaaaaaacg gggccaaaag
      481 gccccggtag tgtacgacag tcttacagtc ccagcaggcc gcaagcgtaa ccagcgatac
      541 cgatgacgaa gaagccaacg atgatccaca gcgggttaac ttttttgcgc agcagccaca
      601 tacaagcaaa tgtcagcagc agtggtacaa ggcctggcat taactggtca agaatagttt
      661 gcacggtggt gacacgggtc tgtccatcct gcccggtgat ggtcgaaacc accagcggga
      721 tgttgacatg tgtccacttg ttaaccaatg cccccatgac aaacaggccg aggatagacg
      781 ccccttccgt cagtttttgc aggaagccac cacccatatc tttaacgata tcgatacctt
      841 tggagtaacc atacgctacg ccgtagtaac gggttgccag acgcaccagg ttaaacagga
      901 tgaagaacag cagcggacct aacaggctgc cgctcatcgc gataccggca cccagtgctg
      961 caaataccgg acgtacggtt ccccagaaga tcgggtcgcc tacaccagcc agcggcccca
     1021 tcaaaccgac tttgataccg ttgatagcac cgtcatcgat ctctgcgcca ttagcacgct
     1081 gttcttccag cgccagggtt acgccgagaa tcggtgcagc cacgaacggc tgggtgttaa
     1141 agaactccag gtgacggcga atagcttgtt tacgagcttc gttgttctca gggtagaggc
     1201 gacgaattgc cggtaccata gagaagcaga aacccagtgc ctgcatacgt tcgaagttcc
     1261 atgaaccctg gaagaggtta gaacgcagga atacgccacg aatatcactt tgagtgagtt
     1321 ttttctcggt ggtagtttga gttgtatcaa ccatttcgct cacctgttag tccagttcgt
     1381 tatcgagatc gttgttacca gctgcctgag caggcgcacc ggctacgcgg ttgtatttcg
     1441 ggctaagttg gatgtagagc actgccataa cagtaccaat cacacccaga gcaaccaggt
     1501 taaagttggt gaatgctgcg gttacgaagc cgaggtagaa gaacggcatc aggtagccag
     1561 cacgcatcat gttgataacc atcgcataac caaccaccac gatcatgccc ccggcgatat
     1621 tcagaccatt ggtcaccact tccggaatcg cattcagcat gttctgtact tcgctggtac
     1681 caacagacag cgcaacgatg acggccggaa tagccacacg cattgcttgc aggaacagag
     1741 aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt atcagcagcc ttatccgcag
     1801 cgtgctggaa agcaacggta atagtacgaa cgatgatggt cagtacctgg cccgcagcgg
     1861 ccagagggat tgccagtgcg atacctgcac caatgctctg atgacctgcg ataaccagaa
     1921 tggtagaaat gatagaagcc agagcggcgt caggcgcaac tgcagcaccg atgttcatcc
     1981 agcccagcgc gatcatttcc agcgtaccac cgataataat accggttttc atatccccaa
     2041 gaacgatacc caccagggta cacgcgatta gcggacggtg aaactgaaat tcatcgagga
     2101 ttgatcccat acctgcgata caggctacga taaataccag cacaatttga agagtggtaa
     2161 tctccattgt acttctcctg tttacgtaag acttaagtgt gaaaactgaa tgataatcaa
     2221 cacaatacgt tacttatcga ttttgctgat cagatccatc attttcagtt tcggatcggt
     2281 ggaaacctta cggacttcca gctcaatacc gcgtgcattc agttttttga acgcctcgat
     2341 atctttttca tcaaccgaaa ccgcgttatt cacctgggtt ttaccctgac ggaatgccat
     2401 accaccgacg ttaacagagg tgattttcac gccgccttca acgagacgct ctacatctgt
     2461 tgggttggta aataacagca ttacgcgttc gccagcatat ttcgggttgt tgtagacgcg
     2521 aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc ggaggtgcaa cctgggtgag
     2581 cagtgtctta cgaacggtat ccgcagccac ttcatcacta acaacaataa tacgggagac
     2641 attggtttct ttggtccagc gggtggcgac ctgaccgtga atcagacggt cgtcgatacg
     2701 cgcaaggcca ataaccatgt agtcgtttgg ccccattggt tttgccggag ttggagccgc
     2761 ttttggtgct gcggcagcgg gtgctggcgc ggctttttca accggtttgg ctttcagtgc
     2821 tttcacgcct tcacggcctg tttctactgc cagcgcaacc agttcatcaa agctcgggtc
     2881 atcatcacgg gccattaacg tttccacgag cattggaatg ttaacgcctg caatgacttc
     2941 ataatgctct ttgtcgacga caatgcggct ggcagcattg aacgggctgc ctccccatgt
     3001 atcaacgaga aacagcacgc ctttagtggt gtcgagtttt gccaactgag cgttgtattt
     3061 ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg atccagccga cgttttcctg
     3121 ctcgcctaac agcatttctg ccgttttaag caactgctct gcagcccaac catgtgtgcc
     3181 tataacaata gcaatggtca cttgctacct cctttattat cgttaacacc tcaacgtgcc
     3241 agatgtattt ttgaatcgtc gtccacaatc gaatcgattc agataagggc tacaaccaaa
     3301 aacccctgcg tttcgcgatt tattttagat atcgaaaaaa ttattttatg tgatgaagat
     3361 ccgtaattta actttcgatt atcagaaatt tcgaaaggta aaatatcctt gtcacattcg
     3421 tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc tctgatatgt ttaactcccg
     3481 tttaacaacc atggagtata gggcagtagc ccgcagtatg gatcgtcacc gacgtcattt
     3541 cagcatcagg ccttttaacg cctgcctttc tggcactcta tgccgcacct ttcgtttgca
     3601 ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc tattccttaa gcaggagctt
     3661 gtcatggaat tcttaatgga cccctcaatt tgggcggggc tactcacgct tgttgttctc
     3721 gaaattgtgc tgggtatcga taacctggtc ttcatcgcca ttcttgctga caaactgccg
     3781 ccaaaacaac gcgataaagc gcgtttgctg gggttatcac tggcgctgat tatgcgtctg
     3841 gggctgctgt cgctgatttc atggatggtc acgctgacca aaccgctatt taccgtcatg
     3901 gatttctcct tctccggacg cgatctgatt atgttgttcg gggggatatt cttgctgttc
     3961 aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg atcatgattc cggccacggt
     4021 aaaggctacg ccagtttctg ggtggtcgtc acacagattg tcatccttga cgccgtcttc
     4081 tcgttggatg cggtaattac tgcagtaggg atggttaacc atctgccggt gatgatggcg
     4141 gcggtagtga ttgcgatggc ggttatgttg ctggcatcga aaccgctgac gcgattcgtt
     4201 aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc tgttaatgat tggtctgagt
     4261 ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt acctgtatgc cgcgattggc
     4321 ttctcgatca ttatcgaagt gtttaaccag attgcgcgtc gcaactttat tcgtcaccag
     4381 tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc tgcgtttgat gggcgggaaa
     4441 cgtcaggcca atgttcagca cgatgccgat aacccgatgc cgatcccgga aggtgcattt
     4501 gccgaagaag aacggtacat gattaacggc gtactgacgc tggcgtcgcg ttctctgcgc
     4561 gggatcatga cgccgcgcgg tgaaataagc tgggttgacg ctaatctcgg ggtcgatgaa
     4621 atccgcgagc aactgctctc ttcaccgcac agtctgttcc cggtatgtcg cggtgaactg
     4681 gatgaaatca tcggtatcgt acgtgctaaa gaactgctgg tggcgctgga agagggcgtt
     4741 gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg tcccggaaac cctcgatccg
     4801 atcaacctgc tgggcgtgct gcgtcgcgct cgcgggagct ttgttatcgt gaccaacgag
     4861 tttggtgtgg tacaaggctt ggtcacgccg ctggatgtgc tggaagccat tgcgggtgaa
     4921 ttcccggacg ctgacgaaac gccggaaatc attaccgatg gtaacggctg gctggtaaaa
     4981 ggcggtacag atttgcatgc cttgcagcag gcgcttgatg ttgagcacct tgccgatgac
     5041 gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa atggtcacat tccccgtgtg
     5101 ggcgatgtga ttgatgtagg gccactgcat atcaccatct ttgaagccaa tgattatcgt
     5161 gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg atgaagatga gtaagcgtta
     5221 acgtaacgac ataatgggcg tgatatgtcc attatgccgg gcgggcggcg gttggctgcc
     5281 cgccagccat ttgggaaaat cacgtagcgg catcggctgt gcgtacaaat acccttgcaa
     5341 aatatgcacg ccatgacggc gcaaatactt cgcctgttct tgcgtttcca cgccctccgc
     5401 caccagttca atattcagtc tttgccccag cgcgatgatg atatcggtca ccgtcgagtt
     5461 aaccgcgtca gaacctatag ctgcggtaaa tgacttatca attttcagca cgtcaggacg
     5521 taatgtttca agccaggaaa acgagctgtt gccggtgccg aagtcatcaa tcgccagttt
     5581 gacgttttta cgatgcagct cgcgggcaat ccgataatca acatccagta aggcatcgcg
     5641 ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg ctaaaccagt actgattgag
     5701 atcttttatc agcacaccac ggcgaaaatg gctgggtgcg acgttaatgc caacatgaaa
     5761 ctgactactc atcgggaaaa catggtgctg acgaatggtt tctgccatca catagtgggt
     5821 cagtggcaca attaaatgat gttcttccgc gataggaata aacacatccg gtgaaatcca
     5881 gccctgacgc ggattattcc agcgcagcaa aatctctaca ccaatacatt gctggctgcg
     5941 cgcattaagc aaaggctgac agaacaattc gaactcatgt tgcgccaggc ccagattaat
     6001 ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag gcgatatagc ccaccagtaa
     6061 acttagcagc accgccagcg gtaattgtgt tggaagatag tgccatgcca gcgccgtagc
     6121 ccccggacca tttacgttaa tggtaaaagg aaagctttgc gaagaaacct gatagttttc
     6181 attgtcttca ggttgcggaa gggaatctac cagcccatta ccatagagca aatgccgttt
     6241 gtccaccgtc aggctggcgc tactgatttg cggcagttgt ggctcaagga gcattgccgt
     6301 cagtaagtcg atgttaatca tctctatgac cccagcattg ctactgcccg ctgcaggcgt
     6361 ccattgaatc aaaaccggac tgcctttaat cagggcacgg tcgatcgtca ggcgtaaaag
     6421 tggttgcggt gcaggaagtt cagccagaat gtccacgacg ggcacattgc gataaccaaa
     6481 aatgctggag caataaagtg tgccgtcttg caccaggcca atggatcgca cagtttggag
     6541 ttttgccgcc tgtttacgta atggcaaatg ggcgacagag cagggcagac caatcagcgg
     6601 aagcaagact ttgctacctg cctgtagggg aagcagtact ttatctaatt cctctacagc
     6661 gtgattggcg aattgtacta cccgttgttg atttaagtta cgctgtgaaa taaatcgcac
     6721 gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta caaacaatca ttcgattacg
     6781 gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg gccaccctgt aagcgtatgg
     6841 cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa tattacagca gacgagaaag
     6901 cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa gaagtattag tcacactgga
     6961 ctttgattgc cagaccaccg cgtgaggttt cgcggtattt ggcgttcatg tccttaccgg
     7021 tttcgtacat cgtttcgatg accttatcca gcgagacgcg cggtgcactg gtgcggcgca
     7081 gagccatccg tgcggcgtta atcgccttca cagaggcaat ggcattacgc tcaatgcacg
     7141 gcacctgaac ctgccctgca accgggtcgc aggttaaacc aaggttgtgt tccatgccaa
     7201 tttccgccgc cacgcaaacc tgttccgggc taccgcccag cagttctgca agacccgcag
     7261 cagccattga acaggcaaca cccacttcgc cctggcaacc aacttccgca ccggaaatag
     7321 aggcgttcat tttatacaat gcaccaatcg cgcccgctgc cataaagtaa cgggtataga
     7381 tgtctgggct gaccgattca ataaagtggt catagtaagc cagcactgcc ggaacgatac
     7441 cgcaggcacc gttggttggc gcagttacca cacgaccacc ggcggcgttt tcttcgttaa
     7501 ctgccagcgc aaacatgttt acccagtcaa tgacattcat cggatcgtta gacagtttat
     7561 cgctggaaac taacatccgg cgcagggcag acgcacgacg tggcacgcgc agcgggcctg
     7621 gcagtacacc ttcggtgttc atcccgcgat cgatacatgc ctgcatggtt tgccagacat
     7681 gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc cagttcgttc tgcatagcga
     7741 gaccagacag cgaatagccg gtttcattac agtacgcgag cagttcggtg gcagatttga
     7801 acggatacgg cacgctcact tcgttggcag catcctgacc aaagtgttct tcatcgacga
     7861 taaaaccgcc gccgatggaa taataagttt tgctgtagac gacttcatcg ccgttatagg
     7921 cgtggatttg cataccgttt tcatgcagcg gcaggttgcc gttatgaaaa cgcatcccgt
     7981 tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag cagcagacgt tcgcgctctt
     8041 ttacgtcgcg aataaaaccg ggaatactgt cgatatccac ggtggcaggt tcgttacctg
     8101 caagacccat aataatggcg atatcggtgt ggtggccttt acccgtcagc gacagtgaac
     8161 cataaacgtc cacggcaacg cgagtaacgc tatccagtaa gcctttttcg accagatcat
     8221 cgacgaactg tttacccgcc ttcataggcc ctacggtatg ggaagatgag ggaccaatcc
     8281 ccaccttaaa catgtcgaat agactaatca cgataatact cctgacaagg tgactggact
     8341 tccagtaacg aactaataac agcgcatagt gtaagaggga acgcggcgac tggcttaact
     8401 attcacatga attaaactaa tcaaaaaccg cgatttaact aatattgcga tgattgtctc
     8461 aagtagtgtt ttgtagatgt aaagataagt atagtcaggg ggtcacacca atttgcagcg
     8521 ccagctcacg aattatgcct gcggtcattc cccatacaaa atactgttcg taccaggaca
     8581 gccatacccg atgtgaatcg ccacggcggt agatatctaa agggtgataa cgacccagat
     8641 gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac ttcatcttca ctggcgcgat
     8701 acggcagatc gggcgggata atgccgacca ctggggttac ttgatagcca gttacgctat
     8761 cgacgggcgg cagcacgccg ataacttcaa cggcggaagg cggtatagcg acctcttctt
     8821 cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt gtcatcgact gcacctccag
     8881 ggaatgccac ttgtccagcg tgtttacgca gatgaattga acgctgagtc agcaacaacc
     8941 ccggttgcgg tcgacggacg atggggatta acacggcagc ctgacgatga tttaggggtt
     9001 cccggttaat ttgcgggcgc aaaagttgaa agcgagataa aaaatcatca agcgtcaggc
     9061 tacggtattc cacgtcttac ttctccagtt ggcgtaatat cttattaact ttatcaaaag
     9121 tttcctgata ttccgcttct tcctggctat cggcgacaat tccaccgccc gcagagcagt
     9181 atatttgtcc gttaatggca gtcagcgtgc ggatagtaat gctggtatcc atgttgccgc
     9241 aaaagctcaa atagccaatg ctgccgcacc aggcattacg tcgctgcggt tccagttcgt
     9301 cgataatttc catagcccgt actttcggag ccccggttat tgagccacca ggaaaagctg
     9361 cgcgcagcag atcgctggcg tgtaactgtt ctggtagtcg cgccgttata gtgctgacca
     9421 gatgatgcac ggcagggaag ggttccacca cgaagagctc tggtactttt accgaaccgg
     9481 ctacggcaac acgaccgata tcattacgca ttaaatcgac aatcatcaga ttttcggcac
     9541 gatctttcgc tgagttcgcc agtttttctg cttgtttgct atcttcctga ggatcgggca
     9601 ggcgtggtag cgtgccttta atcgggcgag tctggatttc actattatca caaagaataa
     9661 accgctctgg cgaaaggctt aaaattgcac cctgttcaag acgtaaaaaa gcgctaaatg
     9721 gcgcgcggtt ggcctgatta agctgaagga atgcctgcca ttcatcgcca gaataggtcg
     9781 catgaaaacg ctgggcgaga ttcacctgat agcaatcacc gctgtgcaga tattcctgta
     9841 cctggcgaaa tttttcgccg tactgctcgt gggtcatatt ggattgccag tcggaagtga
     9901 gcgtgaaatc ttcctgcggc gagaattgct ggctttccag ccaggcccga cgggcattga
     9961 catcattatg actcagcaaa gaaactgtat gacgctggtg gtcgacaatg agcgcccaat
    10021 cgtagatacc cactgccata tccggcagaa cgatatcttg ttccgcaatt tctggcagtg
    10081 actcaaaacg gcggcccaga tcgtagccaa acaaccccag tgcgccgccc tgaaatggca
    10141 aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag cacctgctgg agcacctgta
    10201 gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact aacaacggtt tctttaccta
    10261 aagtggttaa agtgcaaatc ggatcggcga ccacaatatc aaagcggcta tacggatgat
    10321 cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg gcttaagcgg gagaaataaa
    10381 attcagcggc gtcctgacgc cagggtaaag taatcacagt gggagataac gtcttcatca
    10441 gtcctgactc tactggctat gtgccgggta gcatgaaata attagcgcca acaatttagc
    10501 aggagttaac aatgtttgca ggtttacctt cactcatcca tgaacagcag caaaaagctg
    10561 tcgagcggat ccaggaactg atggcacaag ggatgagtag cggccaggca atcgcgctgg
    10621 tggcagaaga gctgcgtgcc aaccactccg gcgagctaat cgtggcgcgt tttgaggatg
    10681 aagacgagta acacactaat ccgtatcgat ttcgttatag aagccgatga tcatttgatt
    10741 gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc ctgacccatt tatgctgata
    10801 taaatttaac tcagaccagt gcttttagtt cttcctgcat atatgttgaa aagtattcag
    10861 gatttttaat cacagagcgt tcacctgtct ccattttttt tgcccatcca accatttttt
    10921 ccgtgaaccc agggttccag ccttcctggt cagctcgcgc cgtaatatca gcggcactcg
    10981 cgggtactcc cagttctttt aaatatttaa agattccctt cgccgtgctt tcattcatcg
    11041 aacttggtgt tggcgctgat acatccatgg caccaatgaa atccagggct ttatctatta
    11101 cagcaggcat attcttatcc ttaaaattaa ccatgttatc gataaggcta tatatgacct
    11161 taatttgcat ctaatatcaa agggattata acgatatggc acgagttaac ctgttgacat
    11221 tcaggtgttt taaaggtagc agctcatggt gtgttatcca gtaagatgga gaaaattaac
    11281 ggtcagcccg cacgctataa ccagattgtt cagctaagaa agaaaaccag agttgctaat
    11341 caaagggcga acacaacggc atggttatgt ttatttatat agttagtttt atagataaaa
    11401 ttgaaattat aaatataaat aatgaattag cagaagcatc aacagtaata tcttattctc
    11461 tggtcgattt atgtaaaacc gaccagtatt atcgataaat tatcgtctga cttattttgc
    11521 ttcgataaag cttacaccgc agccacaatc ttaatttcaa ctttatactt cgggttcatc
    11581 aaacccgctt gtaccgtgca gcgcaccggc gcatgacccg cgacaaccca ggcatcccac
    11641 gctttattca tcgccgcgaa gtcgttttta tcggccagga aaatggtggc atccagaatg
    11701 ctcgatttat tgctgccctg tttttccagc acggcgtcaa tctgtgccag cgtgttggcg
    11761 gtttgctcaa aggcatcggc gtcgaggttt tccggtacac cagtgtagta gagcgtgttg
    11821 ttgtggatta ctacatcaga ccagcgggct tcagcatcga tacgaacgat agtcatcatt
    11881 ttgtcctcat tatacttcca ttttcgtgct gcaaagactg ccacaatgtg aggttgccgt
    11941 caccacttca actggcgaaa gcgccctcat cctgacataa tccctgttca aatcaacagg
    12001 gggtagtgtg acggacgatt ttgcaccaga cggtcagctg gcgaaagcga taccaggctt
    12061 taagccgcga gaaccacagc gacagatggc ggtagccgtc acccaggcga tagaaaaagg
    12121 ccagccgctg gtggtggaag caggaaccgg tacgggcaaa acctacgctt acctggctcc
    12181 tgcgctgcgg gcgaaaaaga aagtcattat ctcgaccggc tcaaaagcgt tgcaggatca
    12241 gctctacagc cgcgatttgc caacagtctc aaaggcattg aaatatacgg gcaacgtggc
    12301 gctgctgaaa gggcgctcaa actacctctg cctcgaacgt ctcgaacagc aggcgctggc
    12361 ggggggcgat ctgccggtac aaatcttaag cgatgtgatc ctgctgcgct cctggtctaa
    12421 tcaaatagtc gatggtgata tcagcacctg cgtcagcgtg gcggaagatt cacaggcgtg
    12481 gccgctggtc accagcacca acgacaactg tcttggcagc gactgcccga tgtataaaga
    12541 ttgctttgtg gtcaaagcac gtaaaaaagc gatggacgcc gatgtggtgg tggtaaacca
    12601 tcatctcttt ctggcggata tggtggttaa agagagtgga tttggcgagt tgatccctga
    12661 agcggacgtc atgatcttcg acgaagccca ccagctaccg gacattgcca gccagtattt
    12721 tggtcagtca ctctccagtc gacaactgct cgacctggca aaagacatca ccatcgccta
    12781 ccgcaccgaa ttaaaagaca cccagcagtt acaaaagtgc gctgatcgtc ttgcccagag
    12841 tgcgcaggat tttcgtctgc aactcggtga gccaggttat cgcggtaacc tgcgtgagct
    12901 gttagctaat ccgcaaattc agcgggcatt tttactgctc gatgacacct tggaactttg
    12961 ttatgatgtg gcgaaactgt cactggggcg ttccgccttg ctggatgcgg catttgagcg
    13021 cgccacgttg tatcgcacac ggctgaagcg gctaaaagag atcaatcagc cgggctacag
    13081 ctactggtac gaatgcactt cgcgccattt tactctggct ctcacgccgc tcagcgtggc
    13141 ggataaattc aaagagttaa tggcgcaaaa gcccggtagc tggatcttca cctcagcaac
    13201 gctgtcggtg aacgacgatc tgcatcattt cacctcgcgg cttgggatag aacaggcgga
    13261 gtcgttgcta ttgccaagcc cgtttgatta cagccgccag gcgttactct gtgtgccgcg
    13321 caatctgccg caaaccaacc agccaggttc tgctcgccag ttagcggcaa tgctgcgacc
    13381 gatcatcgaa gctaacaacg gtcgttgttt tatgctttgt acctcgcacg ccatgatgcg
    13441 cgatctggcc gagcagttcc gcgctaccat gacgcttccc gttttgttgc agggggaaac
    13501 cagtaaaggc caactgttgc agcagtttgt cagtgccggt aatgcgcttc ttgtggcaac
    13561 cagtagtttc tgggaagggg tggacgtgcg tggcgataca ttgtcattgg taattatcga
    13621 caaattgccg tttacctcgc cggatgatcc actgttaaaa gcgcgcatgg aagattgtcg
    13681 tttgcgcggt ggcgacccgt tcgatgaagt gcaactacca gatgccgtca ttactctcaa
    13741 acagggggta ggtcgactga ttcgcgacgc cgacgatcgt ggcgtgctgg tgatttgtga
    13801 ctatcggctg gtgatgcgtc cttacggcgc gacgtttctc gccagtctgc cgcccgcgcc
    13861 acgcacccgt gacattgccc gtgcggttcg tttccttgcg ataccatcct ccaggtaatt
    13921 tgttacggag tatggtatga tgcgcgcctt tttattgatc taagcacgaa gacccatgcg
    13981 aattctggct atcgataccg cgacagaggc ctgctctgtc gccctgtgga acgacggtac
    14041 tgtcaacgct cattttgagc tttgccctcg tgaacatact caacgaatct taccgatggt
    14101 gcaggatatc ctgaccacca gcggaacttc cctgactgat attaacgctc tggcttacgg
    14161 gcgcggcccc ggtagcttta ccggcgtgcg cattggtatt ggcatcgcgc aagggctggc
    14221 gcttggcgcg gaattaccga tgattggcgt ctccacgcta atgacgatgg cgcaaggggc
    14281 gtggcgcaaa aacggcgcaa cacgcgtgct gtcagccatt gatgcgcgaa tgggcgaagt
    14341 ttactgggcc gaatatcagc gtgatgaaaa cggtatctgg cacggtgaag aaaccgaagc
    14401 cgtactcaaa cccgaactcg ttcatgaacg aatgcaacag ctttccggcg aatgggtgac
    14461 ggtagggacg ggctggcaag cctggccgga tctcggtaaa gagagcgggc tggttttgcg
    14521 cgatggcgaa gtgttactcc ccgctgcaga agatatgctg ccgattgctt gtcagatgtt
    14581 tgccgagggt aaaacggtgg cggtggaaca tgccgagccg gtttatttgc gtaacaacgt
    14641 cgcatggaag aaacttccgg gcaaagaatg aatcttaaga gtatgccctg agaaaaaaag
    14701 gagtcgcatc atggcggttc aaaagaatgt tatcaaaggc atactggcag gtacgtttgc
    14761 gctaatgctg agcggttgtg tcactgtgcc ggacgccatt aaaggcagca gtaccacgcc
    14821 gcaacaagat ttagttcggg tgatgagtgc gccgcagctg tacgttggtc aggaggcacg
    14881 ctttggtggc aaagtggttg cggtacaaaa ccagcaaggg aaaacccgcc tggaaattgc
    14941 taccgtaccg ctggacagcg gagctaggcc gacgctggga gaaccttctc gcggtcgcat
    15001 ttatgccgat gtgaacggtt ttctggaccc ggtggatttc cgtggacaac tggttacggt
    15061 agtcgggcca atcaccggtg cggttgacgg caaaatcggc aatacgccct ataaatttat
    15121 ggtgatgcaa gtaacgggtt acaaacgttg gcatttaacc cagcaggtga ttatgccgcc
    15181 tcagccgatt gatccatggt tttatggcgg tcgtggctgg ccctatggct acggcggatg
    15241 gggctggtat aatcccggcc ccgcgagagt acaaacagtt gtaaccgaat aattgcttgt
    15301 ttttaaagaa tgcagaaaca gcggctggtc cgctgtttct gcattcttac ggtaaagata
    15361 aaaataaata gtgacgcgct tcgcaacctt ttcgttgggt aattatcaag ctggtatgat
    15421 gagttaatat tatgttaacg gcgtgtatat catttggggt tgcgatgacg acgaacacgc
    15481 attttagagg tgaagaattg aagaaggttt ggcttaaccg ttatcccgcg gacgttccga
    15541 cggagatcaa ccctgaccgt tatcaatctc tggtagatat gtttgagcag tcggtcgcgc
    15601 gctacgccga tcagcctgcg tttgtgaata tgggggaggt gatgaccttc cgcaagctgg
    15661 aagaacgcag tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg aagaaaggcg
    15721 atcgcgttgc gttgatgatg cctaacttat tgcaatatcc ggtggcgcta tttggcattt
    15781 tgcgtgccgg gataatcgtc gtaaacgtta acccgttgta taccccgcgt gagcttgagc
    15841 atcagcttaa cgatagcggc gcatcggcga ttgttatcgt gtctaacttt gcccacactc
    15901 tggaaaaagt ggttgataaa accgccgttc agcacgtaat tctgacccgt atgggcgatc
    15961 agctatctac ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgcttgg
    16021 tgccgaaata ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc
    16081 ggatgcagta cgtcaaaccc gaactggtgc cggaagattt agccttcctg caatacaccg
    16141 gcggcaccac aggtgtggcg aaaggcgcga tgctgactca ccgcaatatg ctggcgaacc
    16201 tggaacaggt caacgccacc tatgggccgt tgctgcatcc aggtaaagaa ctggtagtaa
    16261 cggcactgcc gctgtatcac atttttgccc tgaccattaa ctgcctgctg tttatcgaac
    16321 tgggtgggca gaacctgctt atcactaacc cgcgtgatat tccagggttg gtaaaagagt
    16381 tagcgaaata tccgtttacc gctatcactg gcgttaacac cttgttcaat gcgttgctga
    16441 acaataaaga gttccagcag ctggatttct ccagtctgca tctttccgca ggcggtggga
    16501 tgcctgttca gcaagtggtg gcagaacgct gggtgaaact gaccggacag tatctgctgg
    16561 aaggttatgg cctgaccgag tgcgcgccgc tggtgagcgt taacccgtat gatattgatt
    16621 atcatagcgg tagcatcggt ttgccggtgc catcgacgga agccaaactg gtggatgacg
    16681 acgacaacga agtaccaccg ggtcaaccgg gtgagctttg tgtcaaagga ccgcaggtga
    16741 tgctgggtta ctggcagcgt ccggatgcta cagatgagat catcaaaaat ggctggttac
    16801 acaccggcga catcgcggtg atggatgaag aaggattcct gcgcattgtc gatcgtaaaa
    16861 aagacatgat tttggtttcc ggttttaacg tctatcccaa cgagattgaa gatgtcgtca
    16921 tgcagcatcc tggcgtacag gaagtcgcgg ctgttggcgt accttccggc tccagtggtg
    16981 aagcggtgaa aatcttcgta gtgaaaaaag atccatcgct taccgaagag tcactggtga
    17041 ctttttgccg ccgtcagctc acgggataca aagtaccgaa gctggtggag tttcgtgatg
    17101 agttaccgaa atctaacgtc ggaaaaattt tgcgacgaga attacgtgac gaagcgcgcg
    17161 gcaaagtgga caataaagcc tgagcgttaa gtcagtcgtc agacgccggt taatccggcg
    17221 ttttttttga cgcccactaa agagaaaaca atttgaatta ccaaatgatt accacggacg
    17281 atgcgctggc ttctttgtgt gaagccgtcc gtgtctttcc ggcgatagcc ctggatactg
    17341 aatttgttcg tacgcgcact tattacccgc agctggggtt gattcaactt ttcgatggcg
    17401 agcatctggc gctaattgat cctctcggga tcaccgactg gtcaccgctg aaagcgatcc
    17461 tgcgcgatcc gtccatcaca aaatttctcc atgcaggcag tgaagatctg gaagtgttcc
    17521 tcaatgtctt tggcgaatta ccacaaccct tgattgacac gcaaatcctt gctgccttct
    17581 gcggacgccc gatgtcatgg ggtttcgctt ccatggtgga agagtattcc ggcgttacgc
    17641 tggacaagag tgaatcgcgc accgactggc tggccagacc gctgaccgaa cgtcagtgtg
    17701 aatacgcagc ggcggatgtc tggtatctgt taccgatcac cgccaagctt atggtagaaa
    17761 cggaggcctc cggctggcta cctgcggcgc tggatgaatg ccgcctgatg caaatgcgtc
    17821 gtcaggaagt cgttgcgccg gaagatgcct ggcgtgatat caccaatgcc tggcagttac
    17881 gcacacgcca actggcctgt ctgcaactgt tagccgactg gcgactgcgc aaggcgcgag
    17941 agcgtgatct ggcggtgaac tttgtcgtgc gtgaagagca tttgtggtcg gtcgcgcgtt
    18001 atatgccggg aagtttaggc gaactggaca gcctgggttt atccggtagc gaaatccgct
    18061 ttcacggtaa aacgctgctg gcgctggtgg aaaaagcgca gacattgccg gaagatgcct
    18121 taccgcagcc gatgcttaac ctgatggaca tgcctggtta tcgtaaagcg tttaaagcga
    18181 ttaagtcgct gattactgac gtgagcgaaa cgcataagat cagcgccgaa ttgctggcat
    18241 cgcgtcggca aatcaaccaa ctgctgaact ggcactggaa actgaaaccg cagaacaatt
    18301 tgccggagct gatttccggc tggcgtggtg agctgatggc ggaagcatta cacaatttat
    18361 tgcaggaata tccgcagtaa aatcttccga agccggactg ggcgcgctca gcgccacatc
    18421 cggctttggc aaactacaaa tccaacacca ggcgtttacc cttcgcacgc gaacaacaga
    18481 tcaacatact ttgctggctg gcacgctctt catcgctaaa atactgatcc cgatggtcag
    18541 cttcaccttc cagtattgcc gtttcgcagg ttccgcatac cccttcacga cataaacatt
    18601 ccactttcgc ggctttatta ttttcaataa cctgcaaaat agtcatctct tccggcacca
    18661 caaactcttt tccggaacgg gcaagcacca gggtaaatgc atcgccggtt ttgtcttcga
    18721 tagcaaattg ctcaaagtgc agtgtatcgg cggcgatgtc cagacgcgca gcttcacttc
    18781 ttaccgcttc aattagcgcc tcggggccac aggtataaac gtgtgtgcca ggttcgatat
    18841 ccgccaataa tcgcgctaat tccagtcgtg ttccggttga tgaatgatgc aaatggactt
    18901 tctcagcctg cggatgctgg actagctcag cacgaaatgc gcaactttct ggatttcgcg
    18961 agcagtaatg tagctgccag tcgatgtcgc tgtgttgcaa ctctgccatg tgcgacagga
    19021 aaggggtgat accaataccg cccgcgataa acagatgctt tctggctgag ggaattagcg
    19081 caaacaggtt attaggcgtt gagatcgtta accgatcgcc cacttttacc tgctgatgca
    19141 aaaagcggga accgccgcgc gagttttctt ccagtcgaac ggcaatctga taactagagg
    19201 tgttatgcgg tgaactcagt agtgaatacg cattgctgta ctggttatca ccatcgctca
    19261 tctgcactag gatgtgactt cctccggtga acgcaggcag cggtttgcca tcggttgcca
    19321 ccagcgtgaa gcgtttcacc tgttcggtaa ggggttcaac ctggctcacc tgtacttcaa
    19381 acatttgata gtctgacatt gctgtactcc atcaaaaatc agcccagcgg cggaaaatac
    19441 cgccgccgat gtcattagtc cttaaacacc tgcgccagca gattatggaa atgggcgata
    19501 ccatgttcgg aaatgccact accgctactg tcggccatga tgcgcccctg accacgatag
    19561 ccacgcgatt tcagcccttt ctgtacgctt tcaaccagac gtaaatcttc cggatggaac
    19621 acatcgcgat accactcaat cagcgatttt tgctcgtcgg ttaactcttc attggtgaag
    19681 taaatatcgt agttttgcag ggtagtttca gaatccaccg ggaattcata aatgaccgtc
    19741 atcatccctt tgatcggggt gacgttcagc atcgtgcacg gccacagcca gaaaccgtgg
    19801 aatgccgcat ccgtaccctc ttcaaattta aacgactgtt cggacggttt ggcgaaaccg
    19861 tattgcagcg tccagttacc gtgcatggtg tgccagtaac gatcaacctg tacggagtcg
    19921 gagaaaccta gatgcgccgg accacagtga tagcactcga gatagttatc gacaatgttc
    19981 ttccagttgg caggcgtgcg ggtggtaaag cgggccgcta gtttcagatt gtggacttcc
    20041 gggcaggctt ccagcacttt cgcgcccaga ccggtgtatt gattcacttg aagtacgaaa
    20101 aaaaaccggg aggacattgg attattcggg ttctgatggg attagatttg gtggggcttg
    20161 caagcctgta gcgcaaattt tagtcgttaa tcaatgaaac gcgaaagata gtaaaaaatt
    20221 gcttttgttt cattgaaaat acgaaaaaca aaaacactgc aaatcatttc aataacagct
    20281 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtaacc aaatgactca tatcacaaat
    20341 cagctcatgc cgtttaggta tgttacatgt gtgattatgt gaggtgaagt atgttttagc
    20401 tggttcatgg ttgttatacg gcttttttta catcttgtgg ttcttgtgaa ggagctacaa
    20461 gaccttcctg ttcataaata ccatactttg acaaaatctt tttacgagta tctgttggta
    20521 atgattcaag cattttggct gtttgtaagg tttgctgcga aataccttgc aaatcagcct
    20581 ggatggaatc atcggaagaa aaaatctcag gcataagagc ttcaaatcct ttctttctga
    20641 acaactcaat cgcagaatgc agttccttgt cctctaacaa ttcgacaatt gcgatcagtt
    20701 tagttttcaa tgtgctgcgg tcaggttgag aatttttatt ctcttgatta tttttatgct
    20761 caaccattgg catgctctga ctttggataa accactctat tggacgccct gttgcgtagg
    20821 ctatttgtgc taatcgatct aaagacgggt atgtcttgcc agacaggtag tttctgataa
    20881 ccccatcaga aagtccgcac ttagctgcaa acgcccggct tgattctcca ttcattgcct
    20941 cttcaaggcg ttccttgaaa cgcattattt ccgggggcat aaaaatgcgc tctgcatttt
    21001 tgctttcttt ttcacctgtc atgcccaatc tctctgttat ataaagaatt ttacagaatt
    21061 ctttgatttg cactggttaa aataaaaatg cgcatagctt ttgaattgag catttttctg
    21121 cgctatcgtt tttcccgata gcggtagccg gtatcagtta ccgaaaacgt taagagagta
    21181 aggatcgcag aatgtcagca aaaaaccaag actggcacgc agagcagata aaggctgctg
    21241 ttcgtatgaa ggggaagaca ctatctcaat tatccagaga
//
LOCUS       DAJTUM010000067        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTUM010000067 DAJTUM010000000
VERSION     DAJTUM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            18..791
                     /locus_tag="OO471_002682"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO471_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO471_002683"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO471_002683"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6243893.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO471_002684"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO471_002684"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6243894.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO471_002685"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO471_002685"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6243895.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO471_002686"
     CDS             complement(4989..7223)
                     /locus_tag="OO471_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6243896.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO471_002687"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO471_002687"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6243897.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO471_002688"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO471_002688"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6243898.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO471_002689"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO471_002689"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6243899.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO471_002690"
     CDS             complement(12978..14318)
                     /locus_tag="OO471_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6243900.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO471_002691"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO471_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6243901.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO471_002692"
     CDS             complement(16106..16555)
                     /locus_tag="OO471_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6243902.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO471_002693"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO471_002693"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6243903.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO471_002694"
     CDS             complement(17355..17684)
                     /locus_tag="OO471_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6243904.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO471_002695"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO471_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6243905.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO471_002696"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO471_002696"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6243906.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO471_002697"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO471_002697"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6243907.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5067 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTUM010000068        20711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTUM010000068 DAJTUM010000000
VERSION     DAJTUM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO471_002698"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO471_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6243908.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO471_002699"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO471_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6243909.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO471_002700"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO471_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6243910.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO471_002701"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO471_002701"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6243911.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO471_002702"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO471_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6243912.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO471_002703"
     CDS             complement(8217..9110)
                     /locus_tag="OO471_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6243913.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO471_002704"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO471_002704"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6243914.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO471_002705"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO471_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6243915.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO471_002706"
     CDS             complement(10241..11473)
                     /locus_tag="OO471_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6243916.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO471_002707"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO471_002707"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6243917.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO471_002708"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO471_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6243918.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO471_002709"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO471_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO471_002710"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO471_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6243919.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO471_002711"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO471_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6243920.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO471_002712"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO471_002712"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6243921.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO471_002713"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO471_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6243922.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4982 a         5726 c         5031 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cagtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt g
//
LOCUS       DAJTUM010000069        20693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTUM010000069 DAJTUM010000000
VERSION     DAJTUM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            83..352
                     /locus_tag="OO471_002714"
     CDS             83..352
                     /locus_tag="OO471_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6243923.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            346..711
                     /locus_tag="OO471_002715"
     CDS             346..711
                     /locus_tag="OO471_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6243924.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO471_002716"
     CDS             complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO471_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6243925.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1538..2221
                     /gene="gntX"
                     /locus_tag="OO471_002717"
     CDS             1538..2221
                     /gene="gntX"
                     /locus_tag="OO471_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6243926.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2280..2855
                     /gene="nfuA"
                     /locus_tag="OO471_002718"
     CDS             2280..2855
                     /gene="nfuA"
                     /locus_tag="OO471_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6243927.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3216..4532
                     /gene="gntT"
                     /locus_tag="OO471_002719"
     CDS             3216..4532
                     /gene="gntT"
                     /locus_tag="OO471_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6243928.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO471_002720"
     CDS             complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO471_002720"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6243929.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TTTKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO471_002721"
     CDS             complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO471_002721"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6243930.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9676..12381
                     /gene="malT"
                     /locus_tag="OO471_002722"
                     /pseudo
     CDS             9676..12381
                     /gene="malT"
                     /locus_tag="OO471_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OO471_002723"
     CDS             complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OO471_002723"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6243931.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OO471_002724"
     CDS             complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OO471_002724"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6243932.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14851..16449
                     /gene="rtcR"
                     /locus_tag="OO471_002725"
     CDS             14851..16449
                     /gene="rtcR"
                     /locus_tag="OO471_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6243933.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16431..17189)
                     /locus_tag="OO471_002726"
     CDS             complement(16431..17189)
                     /locus_tag="OO471_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6243934.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OO471_002727"
     CDS             complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OO471_002727"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6243935.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OO471_002728"
     CDS             complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OO471_002728"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6243936.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18597..20102
                     /gene="glpD"
                     /locus_tag="OO471_002729"
     CDS             18597..20102
                     /gene="glpD"
                     /locus_tag="OO471_002729"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6243937.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20371..20601)
                     /locus_tag="OO471_002730"
                     /pseudo
     CDS             complement(20371..20601)
                     /locus_tag="OO471_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4894 a         5693 c         5214 g         4892 t
ORIGIN      
        1 gagtcagtag atactctacc ctgcttaagt gctactttgt gctatcttgc atgtaacaca
       61 aagtagcact tagggaactg atatgcagac agtgaaatta cggcaacaag gaggagccat
      121 gatcgttact atcccacgag atcttgccat cgatcttggc tggtcaccgg gcacagagct
      181 taccgtggag aaaaaaggcg atagcgttaa tttgcgcgcc acagagcaca aaccgcgtgg
      241 gcgtcttacc gtcgcgcagt tgttgagcca aatcgatgaa agcgaaatta cggagctaaa
      301 tcagtccaca gaaggatggg ctgaagggaa aaaaggtaat gaagcatggt aaaggcacgg
      361 acgccacatc gtggtgagat ctggtatttt aaccctgatc cggttgccgg gcatgaactt
      421 caggggccac attattgcat tgtggtaacg gacaaaaaac tcaacaatgt tttaaaagtt
      481 gctatgtgct gcccgatttc aacaggggca aatgcagcac gttccacagg ggtgacggtg
      541 aacgtcctcc cccgtgatac gcaaaccggt aacctgcatg gcgttgtact ttgtcaccag
      601 ctaaaagccg tcgatcttat tgcccgtggc gctaaatttc ataccgttgc cgatgaaaaa
      661 ttgattagtg aagttatcag taaactggtg aatttaatcg acccacaata attagctctc
      721 acttaccacc aacaccctct gcttcaacgc caccagcagg tgacaaaact cgaccggatg
      781 cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt gaggccaaag
      841 tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg
      901 caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc
      961 cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa
     1021 cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca ggaaccgctc
     1081 cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt
     1141 tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc
     1201 ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca
     1261 gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca tatcagcaag
     1321 tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt
     1381 aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc
     1441 gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat
     1501 ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg
     1561 ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgttcacg
     1621 cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca
     1681 tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc
     1741 cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc ggcgcagtga
     1801 aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac
     1861 cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc gtcactggcg
     1921 tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca
     1981 atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt ttctcagtgc
     2041 ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg tgcaaggtcg
     2101 ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca
     2161 gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta
     2221 gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta
     2281 tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag
     2341 aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg
     2401 tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa tttgacctgc
     2461 tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag atcgactttg
     2521 ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa atgcgtaaag
     2581 tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg cagatcaacc
     2641 cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg
     2701 ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag
     2761 aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg cgcgatctca
     2821 ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc
     2881 cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcaca
     2941 gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata
     3001 atttaaattt accccgctct ggtgattctc aaacgccaaa tgttacccgc atcattcaca
     3061 tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc
     3121 gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg
     3181 acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg
     3241 gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg
     3301 tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct
     3361 ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg
     3421 caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga
     3481 ttgccaaatt tggtaaaaag cacatccagt gggcggtggt gttaaccggt tttaccgttg
     3541 gttttgccct gttctatgaa gtgggcttcg tgctgatgct gccgctggtg tttaccatcg
     3601 cggcttccgc gaatattcca ttgctgtatg ttggtgtacc aatggcggct gcactgtctg
     3661 tgacccacgg cttcctgcca ccgcatccgg gcccgactgc gattgccacc attttcaatg
     3721 ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg
     3781 ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaatcccg gaaggtctct
     3841 acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct
     3901 ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag
     3961 gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca acgctgattg
     4021 ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta
     4081 acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg
     4141 gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga
     4201 tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta
     4261 tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga
     4321 ttgcaacgac gggtgttagc ccagagctga tggttattgc ggtgggttcc ggtagtgtga
     4381 ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta
     4441 tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg
     4501 taggctgtct gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc
     4561 cgacatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
     4621 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
     4681 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
     4741 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
     4801 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
     4861 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
     4921 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
     4981 gatccccgct ctcccagtaa ccacgcagcg ttggcaggtc atgtgtcgcc gcaaccgcca
     5041 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcatggtcg ttttcgaaat
     5101 agagcacttt gtaagagtac acaccgctgc tgcgcagctt accaacaatc tctaccggta
     5161 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
     5221 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
     5281 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
     5341 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
     5401 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
     5461 cgccaaccga tgctttcagg caatatagtt cacggtcaca ccaagtttcc gccccacctt
     5521 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
     5581 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
     5641 cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttcttataca
     5701 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
     5761 gcgcatcaaa ggcagcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
     5821 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
     5881 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc gcctgttgca
     5941 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
     6001 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
     6061 atgggctggc gctctccgga tttgccggat agagcgcatg aatcgggttc aggccaatga
     6121 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
     6181 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
     6241 gtttattcag caaagcctgc ggttcgtaac aacgtttcgg ggcgacaatc acccggcaat
     6301 gcgcgcgctg gtcgtcctgg gtgagtgtta gcgtgtgata accttccggc agcttcgtcg
     6361 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgcgttcct tcttcggtgg
     6421 tcagctgcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
     6481 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggtggta cgttgatgca
     6541 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
     6601 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
     6661 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
     6721 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
     6781 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
     6841 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
     6901 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
     6961 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc ttcacttgtt
     7021 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
     7081 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
     7141 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
     7201 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
     7261 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
     7321 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
     7381 cctgcaggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
     7441 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
     7501 caataccggt acgaactttc acaaactccg ccagacagac tttattcgcc tgcttgatct
     7561 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
     7621 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
     7681 tgcactgttt gatccagcga cgtggggtaa taccgttggt aacgttatgg aatttgttcg
     7741 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
     7801 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
     7861 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
     7921 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
     7981 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
     8041 cgaaagtttt gctggtgatg gcccaggcgt catcccagct catctggtgc tcatcggtca
     8101 gcacgcgcag cagttctgga atcgcgatag ttggatgggt atcgttcagc tgaataactt
     8161 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
     8221 ctaccgaaca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
     8281 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgcct tgctgttcgg
     8341 cgcgcaggaa atcaccgtcg ttaaatttag ttagatcaaa cggatgcgca tgcgtcgcct
     8401 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
     8461 cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcatcact ttaccgccaa
     8521 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
     8581 agtcatccgg cacttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
     8641 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
     8701 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
     8761 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
     8821 gattcaacag gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa
     8881 catgtcgctg attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg
     8941 cttcactcac tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
     9001 aacgctgcca ctgacgtgaa agcacttcct gaaattgctt atcgttaaaa ataggttgtg
     9061 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
     9121 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
     9181 aggaaatgtg atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
     9241 tgccatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
     9301 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
     9361 tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
     9421 ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc
     9481 agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg
     9541 acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg
     9601 ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca
     9661 gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg actcgaccat
     9721 accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg
     9781 ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg ggcggcaggc
     9841 aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga gcgtttcgcc
     9901 agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat atgtgagacg
     9961 atggagcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag
    10021 ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat
    10081 ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcacccta
    10141 gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgaccaa
    10201 ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt
    10261 gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt
    10321 tccggttggg cgacggcact atagctaatc gccctctccg cccggcagaa tacccactca
    10381 gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg
    10441 gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc
    10501 attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga aaacgggcaa
    10561 atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc
    10621 gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg ccagtgggaa
    10681 ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga
    10741 tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat
    10801 attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag
    10861 tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt gttattgcag
    10921 gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct agcccgtgct
    10981 gaacatgaaa tcaaggacat cagagaaggc accatgcatg cagaatttaa cgctctgcgc
    11041 gcccaggtgg cgattaacga tggtaatcag gatgaagcgg aacggctggc aaaactggca
    11101 ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt
    11161 gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctagtgca gcaaaccgaa
    11221 cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt
    11281 gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc
    11341 cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct ggtgcgcatc
    11401 cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc
    11461 gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg
    11521 attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagtt gaaccgtctg
    11581 gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc
    11641 cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat
    11701 acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc
    11761 cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga agaactcaat
    11821 gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat
    11881 caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta
    11941 aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg
    12001 caacaactgc gccagctgat tcaacttaat acgctgccgg aactggaaca gcatcgcgcg
    12061 cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag
    12121 aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactaatccg caccagcccg
    12181 ttgacccaac gtgaatggca ggtcctgggg ctgatctact ccggttacag caatgaacaa
    12241 attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac
    12301 caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag
    12361 atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt ttacatcttt
    12421 acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa accgcaccgg
    12481 caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat gggcgaccgt
    12541 aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga gatattcccc
    12601 caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg cggctaccac
    12661 ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat tttcactttc
    12721 gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat gaatattctg
    12781 ttcatgcagg gaaaaaatcc ccgccagtgt agcgatttca cgctcagcaa catggcgcgg
    12841 cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg
    12901 caaggtgcta aacgatgcca cgggcgagac ttccgttgcc accacaccgc ctccggcagg
    12961 ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg
    13021 ctccagcacc cggcggataa aatccgcagg cggcgccgac gggttatcgg tgccgccgct
    13081 cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac
    13141 cagcgtacaa cttccggcgc taccgatagc aaagcggtaa tcgccgccgc gcacggtgcc
    13201 aggccggaag accagacgct gcgatcccag ctccgcgcct tccaccgttg ccctacaaat
    13261 ttccgcagcc gcttttaccg cggtcagatg ctggcgcagc agccccggtt tcgcccgtcc
    13321 ggctcgaatg ccagtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga
    13381 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
    13441 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
    13501 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
    13561 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
    13621 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
    13681 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
    13741 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
    13801 aaagagctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
    13861 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
    13921 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
    13981 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
    14041 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
    14101 tccgcgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
    14161 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
    14221 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
    14281 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
    14341 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
    14401 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
    14461 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
    14521 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
    14581 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
    14641 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
    14701 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaattta ttgtttctaa
    14761 aaagattata tctttatatc cgtaaacgga gatttcccgc aaagccgatt taccgataat
    14821 gaaatatcgc cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc
    14881 ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcaaaatg gcgtccgtca
    14941 ctctgtatat accagcaaga atcgttggtc atcgatcgac tggaattatt gcacgatgcc
    15001 cgctcgcgct cgctatttga aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca
    15061 gaagtggtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga ggtttacgcc
    15121 tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt
    15181 cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt
    15241 tatctacccg cccggttgat acaatcttca actccgcgca aaaaagaaca gccgcgcggc
    15301 ccaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat cgccagccgc
    15361 tttgccgagg aacggcagca aacgctcgat ttcctcaaat ccggtattgc cacgcgtaac
    15421 ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgattaaatc ccgcgcgccg
    15481 attctgctta acggcccgac cggcgcaggt aagtcatttc tggcgcgacg catcttcgag
    15541 ttaaaacagg cacggcatca gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg
    15601 cgcggcgata ccgcgatgtc gacgctgttt ggtcatgtga aaggcgcgtt taccggggcg
    15661 cgggaatctc gtgaaggatt attacgcagc gccaacggcg gaatgttgtt tcttgatgag
    15721 attggcgaac tgggcgcaga cgaacaggca atgctgctga aagctattga agagaaaacc
    15781 ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat cgccggaacg
    15841 gtgcgcgatt tgcgccagct ggttgccgaa ggcaaattcc gcgaagatct gtacgcgcgg
    15901 atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga tattgaaccg
    15961 aacctggatt atgaagtgga gcgccacgcc acactcactg gtgacagcgt gcgttttaac
    16021 accgaagcgc ggcgtgcctg gctggccttt gcaacatctc cccaggcaac atggcgcggt
    16081 aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac tagcggacgc
    16141 atcactctgg acgtggtgga agatgagata aaccgtctgc gctataactg gcaggagagc
    16201 cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga tctcttcgac
    16261 cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct ttctgctgcc
    16321 ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg
    16381 ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga tcagcacagc
    16441 tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg cggggcgtcg
    16501 gtgtagacgg catcaaccat gctgatgctc cccatattga ccatcgcatt acggccaaat
    16561 tttgaatggt caaccaccag cataacgtgg cgcgagttct caataatggc gcgcttggta
    16621 cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tgtcgatgcc gctgatcccc
    16681 agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc gccaatgatc
    16741 ccgccatcgc ggctacgtaa ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc
    16801 atcaacgtgt tagcaacgtt gagattgttg gtgacaatgc gcaaattgct gtgattgagc
    16861 agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt cgagccattg
    16921 gggatttgct ccgccacttt gcgggcgatg cgctcttttt cttcggtctg ggtggcttta
    16981 cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcgccgcc atgatggcgc
    17041 aggatcaggt tttgctctgc cagctcgttg aggtcgcggc gaatagtctg cgggctgacg
    17101 gagaagtgct ccaccagttc ctcggtactg acataaccct gctgtttaac cagttcgata
    17161 ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt ttcgttttcg
    17221 cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaatccgg cgatgtgtgc
    17281 tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc agatcagcgc
    17341 aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag
    17401 ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc acggcccgct
    17461 gaatttttgc tgcatatagc cgcttaacag ggcgctgata agcgtaatga caattagctt
    17521 accgctaccg aggcgttttt ccaccgcgcc gccgagatac caccaccaaa gcaggttaaa
    17581 gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa
    17641 tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat cgccgagaat
    17701 ttgcatggca ataaacacca ccacgcaggc gatcatcatc acccaggtca ccggacctgc
    17761 gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcaggccac tgccggtatg
    17821 gcctgccagc cagctggccg ccagataacg cggatctgcc gggttttcga gaaaacgcgc
    17881 cagctccgcc cgcacgcgct cggcctggga ctcatccgcc agccagacat cgctttggtt
    17941 atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa acgcctgcgc
    18001 cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt attccacaca
    18061 aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa ctgtcgctgc
    18121 caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg cagcagatac
    18181 tgcgccgccc ctttgctgct attgccgtga taacacatca ccatcaccgg agtgtcaaag
    18241 tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca
    18301 tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc tttttcctgc
    18361 aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg tctctctttc
    18421 tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag ataaacgcca
    18481 taatgttata catatcactc taaaatgttt tttcaatgtt acccaaagcg cgattctttg
    18541 ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag ggcagcatgg
    18601 aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc gcggcagacg
    18661 ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct
    18721 cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac tatgaattcc
    18781 gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct ccgcatatcg
    18841 ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg tggatgattc
    18901 gcattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg ggatcaactg
    18961 gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt
    19021 ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta
    19081 aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt
    19141 ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc
    19201 tggttaacgc caccggcccg tgggtgaaac agttcttcga cgaggggatg catctgcctt
    19261 cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc gtgcataccc
    19321 aaaagcaggc ctacattctg caaaacgaag ataaacgcat tgtgttcgtg atcccgtgga
    19381 tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggtgat ccgaaagcag
    19441 tgaagattga agagagtgaa atcaattacc tgctgaaagt gtataacgcg cactttaaaa
    19501 agcagctaag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg
    19561 atgagtccga ctcgccgcaa gccattaccc gtgattacac ccttgatatc catgatgaaa
    19621 acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac cgaaaactgg
    19681 cagaacatgc gctggaaaaa ctgacgccgt attatcaggg tattggcccg gcgtggacga
    19741 aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac gccgctcgcc
    19801 tgcgccgccg ttacccgttc ctgactgaat cgctggcacg tcactacacg cgcacttacg
    19861 gcagcaacag cgagctgctg ctcggcaatg cgggaacggt aagcgatctc ggggaagatt
    19921 tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa tgggtccgcc
    19981 gcaccgacga tgccctgtgg cgtcgcacaa aacaaggcat gtggctgaat gcggatcaac
    20041 aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg ctggcgtcgt
    20101 aaattaacgt aaggtgatca ggtcagattt caatctggcc tgagactgat gacaaacaca
    20161 aaactgcctg atgcgctacg cttatcaggc ctacgtggtt tatgcaatat attgaatttg
    20221 catggtcttg taggccagat aagacgttca cgtcgcatcc ggcatgaaca aagcgcactt
    20281 tgtcaaaaat ctaacctact ttttaattca ggaaattacc gcaaagccca cgccatcata
    20341 cccaacgtaa caaaactcag gcacgttccc cactcgcccc gagaaaaata gcattaatgc
    20401 gcccagcgtc agcataaaaa tattgagcag aaggtaaatt gttggcgtaa agagtaaatt
    20461 gcaaacctca ggaatagcgg caagcccagc ccccagcatg gcaataaccg ctgcaaaaaa
    20521 ttgcgtcagt attgggatgc cgaaaaaagt cattaccagc gaggtcaaaa tccattttct
    20581 attttgcatt attctttcca ttctttctga atggcgaaat tatactcccc gagtcccctt
    20641 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DAJTUM010000070        20626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTUM010000070 DAJTUM010000000
VERSION     DAJTUM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..579
                     /locus_tag="OO471_002731"
     CDS             <1..579
                     /locus_tag="OO471_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6243938.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO471_002732"
     CDS             597..1235
                     /locus_tag="OO471_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6243939.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO471_002733"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO471_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6243940.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO471_002734"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO471_002734"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6243941.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO471_002735"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO471_002735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6243942.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO471_002736"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO471_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6243943.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO471_002737"
     CDS             complement(5552..6613)
                     /locus_tag="OO471_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6243944.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO471_002738"
     CDS             complement(6610..8007)
                     /locus_tag="OO471_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6243945.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO471_002739"
     CDS             8163..9161
                     /locus_tag="OO471_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6243946.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO471_002740"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO471_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO471_002741"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO471_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO471_002742"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO471_002742"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6243947.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO471_002743"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO471_002743"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6243948.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO471_002744"
     CDS             complement(14232..14855)
                     /locus_tag="OO471_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6243949.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO471_002745"
     CDS             complement(14852..15274)
                     /locus_tag="OO471_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6243950.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO471_002746"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO471_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO471_002747"
     CDS             complement(16089..17141)
                     /locus_tag="OO471_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6243951.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO471_002748"
     CDS             complement(17172..18014)
                     /locus_tag="OO471_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6243952.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO471_002749"
     CDS             complement(18001..18882)
                     /locus_tag="OO471_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6243953.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO471_002750"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO471_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20626)
                     /gene="ycjM"
                     /locus_tag="OO471_002751"
     CDS             complement(20200..>20626)
                     /gene="ycjM"
                     /locus_tag="OO471_002751"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6243954.1"
                     /translation="PAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDE
                     ATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNV
                     SKNIQHVNITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5032 a         5630 c         5017 g         4947 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggt
//
LOCUS       DAJTUM010000071        20612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTUM010000071 DAJTUM010000000
VERSION     DAJTUM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            96..395
                     /locus_tag="OO471_002752"
     CDS             96..395
                     /locus_tag="OO471_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6243955.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO471_002753"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO471_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6243956.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO471_002754"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO471_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6243957.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="OO471_002755"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="OO471_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6243958.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="OO471_002756"
     CDS             complement(2534..2878)
                     /locus_tag="OO471_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6243959.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="OO471_002757"
     CDS             3030..3260
                     /locus_tag="OO471_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6243960.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="OO471_002758"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="OO471_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6243961.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="OO471_002759"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="OO471_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6243962.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="OO471_002760"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="OO471_002760"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6243963.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="OO471_002761"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="OO471_002761"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6243964.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO471_002762"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO471_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6243965.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO471_002763"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO471_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6243966.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO471_002764"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO471_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6243967.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO471_002765"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO471_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO471_002766"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO471_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6243968.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO471_002767"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO471_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6243969.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO471_002768"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO471_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6243970.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO471_002769"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO471_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO471_002770"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO471_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6243971.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO471_002771"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO471_002771"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6243972.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO471_002772"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO471_002772"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6243973.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO471_002773"
     CDS             complement(19399..19827)
                     /locus_tag="OO471_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6243974.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO471_002774"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO471_002774"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6243975.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4973 a         5273 c         5149 g         5217 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat acaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag gcagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaagctg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg at
//
LOCUS       DAJTUM010000072        20538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTUM010000072 DAJTUM010000000
VERSION     DAJTUM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO471_002775"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO471_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6243976.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO471_002776"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO471_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6243977.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO471_002777"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO471_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO471_002778"
     CDS             1631..2341
                     /locus_tag="OO471_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6243978.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO471_002779"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO471_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6243979.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO471_002780"
     CDS             complement(3469..4119)
                     /locus_tag="OO471_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6243980.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="OO471_002781"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="OO471_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6243981.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="OO471_002782"
     CDS             complement(6584..7024)
                     /locus_tag="OO471_002782"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6243982.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO471_002783"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO471_002783"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6243983.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="OO471_002784"
     CDS             7742..8440
                     /locus_tag="OO471_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6243984.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="OO471_002785"
     CDS             8669..9877
                     /locus_tag="OO471_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6243985.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="OO471_002786"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="OO471_002786"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6243986.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="OO471_002787"
     tRNA            11984..12060
                     /locus_tag="OO471_002787"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="OO471_002788"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="OO471_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6243987.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="OO471_002789"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="OO471_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6243988.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16650
                     /gene="dppC"
                     /locus_tag="OO471_002790"
     CDS             15748..16650
                     /gene="dppC"
                     /locus_tag="OO471_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6243989.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16661..17644
                     /gene="dppD"
                     /locus_tag="OO471_002791"
     CDS             16661..17644
                     /gene="dppD"
                     /locus_tag="OO471_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6243990.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17641..18645
                     /gene="dppF"
                     /locus_tag="OO471_002792"
     CDS             17641..18645
                     /gene="dppF"
                     /locus_tag="OO471_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6243991.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18675..19946)
                     /locus_tag="OO471_002793"
     CDS             complement(18675..19946)
                     /locus_tag="OO471_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6243992.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20422..20529
                     /locus_tag="OO471_002794"
     CDS             20422..20529
                     /locus_tag="OO471_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6243993.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4922 a         5082 c         5401 g         5133 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    16141 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    16201 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    16261 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    16321 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    16381 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    16441 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    16501 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16561 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16621 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16681 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16741 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16801 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16861 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16921 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16981 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    17041 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    17101 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    17161 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    17221 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    17281 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    17341 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    17401 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    17461 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    17521 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17581 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17641 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17701 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17761 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17821 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17881 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17941 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    18001 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    18061 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    18121 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    18181 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    18241 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    18301 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    18361 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    18421 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    18481 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18541 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18601 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18661 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18721 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18781 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18841 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18901 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18961 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    19021 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    19081 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    19141 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    19201 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    19261 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    19321 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    19381 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    19441 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    19501 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19561 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19621 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19681 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19741 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19801 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19861 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19921 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19981 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    20041 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    20101 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    20161 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagcaa
    20221 tcgtagaatc agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa
    20281 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgtacctg
    20341 acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    20401 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    20461 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    20521 cggaagtagc gtgtcatg
//
LOCUS       DAJTUM010000073        20521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTUM010000073 DAJTUM010000000
VERSION     DAJTUM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO471_002795"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO471_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6243994.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO471_002796"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO471_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6243995.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO471_002797"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO471_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6243996.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO471_002798"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO471_002798"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6243997.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO471_002799"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO471_002799"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6243998.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO471_002800"
     CDS             4449..4814
                     /locus_tag="OO471_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6243999.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO471_002801"
     CDS             4964..5482
                     /locus_tag="OO471_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6244000.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO471_002802"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO471_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO471_002803"
     CDS             6713..7195
                     /locus_tag="OO471_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6244001.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO471_002804"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO471_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6244002.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO471_002805"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO471_002805"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6244003.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO471_002806"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO471_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6244004.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO471_002807"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO471_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6244005.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO471_002808"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO471_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6244006.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO471_002809"
     CDS             10939..11730
                     /locus_tag="OO471_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6244007.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO471_002810"
     CDS             11751..13037
                     /locus_tag="OO471_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6244008.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO471_002811"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO471_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6244009.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO471_002812"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO471_002812"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6244010.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO471_002813"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO471_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6244011.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO471_002814"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO471_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6244012.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO471_002815"
     CDS             complement(16985..17275)
                     /locus_tag="OO471_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6244013.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO471_002816"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO471_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6244014.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO471_002817"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO471_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6244015.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO471_002818"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO471_002818"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO471_002819"
     CDS             19137..20378
                     /locus_tag="OO471_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6244016.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4815 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAJTUM010000074        20224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTUM010000074 DAJTUM010000000
VERSION     DAJTUM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO471_002820"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO471_002820"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6244017.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO471_002821"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO471_002821"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6244018.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO471_002822"
     CDS             complement(1728..2999)
                     /locus_tag="OO471_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6244019.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="OO471_002823"
     CDS             complement(3182..4387)
                     /locus_tag="OO471_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6244020.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="OO471_002824"
     CDS             complement(4487..4843)
                     /locus_tag="OO471_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6244021.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OO471_002825"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO471_002825"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO471_002826"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO471_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6244022.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OO471_002827"
     CDS             6773..9829
                     /locus_tag="OO471_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6244023.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OO471_002828"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OO471_002828"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6244024.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OO471_002829"
     CDS             10336..10524
                     /locus_tag="OO471_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6244025.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OO471_002830"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OO471_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6244026.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OO471_002831"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OO471_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6244027.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OO471_002832"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OO471_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6244028.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OO471_002833"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OO471_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6244029.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OO471_002834"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OO471_002834"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6244030.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OO471_002835"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OO471_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6244031.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OO471_002836"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OO471_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6244032.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO471_002837"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO471_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6244033.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO471_002838"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO471_002838"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6244034.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5304 a         4857 c         5164 g         4899 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgacaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaacta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gtttgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatt
//
LOCUS       DAJTUM010000075        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTUM010000075 DAJTUM010000000
VERSION     DAJTUM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO471_002839"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO471_002839"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6244035.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO471_002840"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO471_002840"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6244036.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO471_002841"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO471_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6244037.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO471_002842"
     CDS             6038..6658
                     /locus_tag="OO471_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6244038.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO471_002843"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO471_002843"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6244039.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO471_002844"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO471_002844"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6244040.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO471_002845"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO471_002845"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6244041.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO471_002846"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO471_002846"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6244042.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO471_002847"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO471_002847"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO471_002848"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO471_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO471_002849"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO471_002849"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6244043.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO471_002850"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO471_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6244044.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO471_002851"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO471_002851"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6244045.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO471_002852"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO471_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6244046.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO471_002853"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO471_002853"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6244047.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO471_002854"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO471_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6244048.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4682 a         5506 c         5032 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTUM010000076        19375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTUM010000076 DAJTUM010000000
VERSION     DAJTUM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO471_002855"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO471_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6244049.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO471_002856"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO471_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6244050.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO471_002857"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO471_002857"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6244051.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO471_002858"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO471_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6244052.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO471_002859"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO471_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6244053.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO471_002860"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO471_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6244054.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO471_002861"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO471_002861"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6244055.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO471_002862"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO471_002862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6244056.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO471_002863"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO471_002863"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6244057.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO471_002864"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO471_002864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6244058.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO471_002865"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO471_002865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6244059.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO471_002866"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO471_002866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6244060.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO471_002867"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO471_002867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6244061.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO471_002868"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO471_002868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6244062.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO471_002869"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO471_002869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6244063.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO471_002870"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO471_002870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6244064.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO471_002871"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO471_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6244065.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO471_002872"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO471_002872"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6244066.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4770 a         4854 c         5118 g         4633 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 attactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatatgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAJTUM010000077        18804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTUM010000077 DAJTUM010000000
VERSION     DAJTUM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(139..3654)
                     /locus_tag="OO471_002873"
     CDS             complement(139..3654)
                     /locus_tag="OO471_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6244067.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO471_002874"
     CDS             complement(4107..5369)
                     /locus_tag="OO471_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6244068.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO471_002875"
     tRNA            complement(5567..5642)
                     /locus_tag="OO471_002875"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO471_002876"
     CDS             complement(5748..6455)
                     /locus_tag="OO471_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6244069.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO471_002877"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO471_002877"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6244070.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO471_002878"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO471_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6244071.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO471_002879"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO471_002879"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6244072.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO471_002880"
     CDS             complement(11639..11914)
                     /locus_tag="OO471_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6244073.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO471_002881"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO471_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6244074.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO471_002882"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO471_002882"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6244075.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO471_002883"
     CDS             13874..14200
                     /locus_tag="OO471_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6244076.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO471_002884"
     CDS             14384..15103
                     /locus_tag="OO471_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6244077.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO471_002885"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO471_002885"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6244078.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO471_002886"
     CDS             16442..17404
                     /locus_tag="OO471_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6244079.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18804)
                     /locus_tag="OO471_002887"
     CDS             complement(17505..>18804)
                     /locus_tag="OO471_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6244080.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4585 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atcc
//
LOCUS       DAJTUM010000078        18450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTUM010000078 DAJTUM010000000
VERSION     DAJTUM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..1238
                     /locus_tag="OO471_002888"
                     /pseudo
     CDS             1..1238
                     /locus_tag="OO471_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="OO471_002889"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="OO471_002889"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6244081.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="OO471_002890"
     CDS             1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="OO471_002890"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6244082.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="OO471_002891"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="OO471_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6244083.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="OO471_002892"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="OO471_002892"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6244084.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="OO471_002893"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="OO471_002893"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6244085.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="OO471_002894"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="OO471_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6244086.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="OO471_002895"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="OO471_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6244087.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="OO471_002896"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="OO471_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6244088.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="OO471_002897"
     CDS             10439..10654
                     /locus_tag="OO471_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6244089.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="OO471_002898"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="OO471_002898"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6244090.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="OO471_002899"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="OO471_002899"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6244091.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="OO471_002900"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="OO471_002900"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6244092.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="OO471_002901"
     CDS             complement(15390..15929)
                     /locus_tag="OO471_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6244093.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..16463)
                     /locus_tag="OO471_002902"
     CDS             complement(15945..16463)
                     /locus_tag="OO471_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6244094.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16774..16965)
                     /locus_tag="OO471_002903"
     CDS             complement(16774..16965)
                     /locus_tag="OO471_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6244095.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            16983..17135
                     /locus_tag="OO471_002904"
     CDS             16983..17135
                     /locus_tag="OO471_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244096.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17317..>18450
                     /gene="pdeF"
                     /locus_tag="OO471_002905"
     CDS             17317..>18450
                     /gene="pdeF"
                     /locus_tag="OO471_002905"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6244097.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4361 a         4481 c         4992 g         4616 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacattttt taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt
    16501 taagatcatc aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt
    16561 aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat attaatttta
    16621 tatttatttc gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga
    16681 tgaaagaata tctgccgctt tgataatccc aaagagcaaa agcatccgac agcgaagtgc
    16741 cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga
    16801 gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga
    16861 gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg
    16921 actgttaaat cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag gttccttgaa
    16981 gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt
    17041 cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa
    17101 ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa
    17161 tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat
    17221 gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca
    17281 cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata
    17341 aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt
    17401 ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg
    17461 cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc
    17521 accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta
    17581 acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc
    17641 cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag
    17701 atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta
    17761 ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat
    17821 tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg
    17881 gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat
    17941 gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg
    18001 ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca
    18061 ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct gatttcgctg
    18121 ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat
    18181 cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt
    18241 gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg
    18301 cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg
    18361 cgtgatgccc cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt
    18421 aagaactatg gcatcatgct gcggattcaa
//
LOCUS       DAJTUM010000079        18071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTUM010000079 DAJTUM010000000
VERSION     DAJTUM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            256..780
                     /locus_tag="OO471_002906"
     CDS             256..780
                     /locus_tag="OO471_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6244098.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="OO471_002907"
     CDS             861..1535
                     /locus_tag="OO471_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6244099.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4031
                     /locus_tag="OO471_002908"
     CDS             1551..4031
                     /locus_tag="OO471_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6244100.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            4047..5080
                     /locus_tag="OO471_002909"
                     /pseudo
     CDS             4047..5080
                     /locus_tag="OO471_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="OO471_002910"
     CDS             complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="OO471_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6244101.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5719..6540)
                     /gene="rcnA"
                     /locus_tag="OO471_002911"
     CDS             complement(5719..6540)
                     /gene="rcnA"
                     /locus_tag="OO471_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6244102.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRGERNWLENMHGHDYEHHHHHHDHEHHHDQGHHHHHEHGEYQDAHARAHANDI
                     KRRFDGREVINWQILLFGLTGGFIPCPAAITVLLICIQLKALTLGATLVVSFSIGLAL
                     TLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6661..6933
                     /gene="rcnR"
                     /locus_tag="OO471_002912"
     CDS             6661..6933
                     /gene="rcnR"
                     /locus_tag="OO471_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6244103.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7025..7121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7156..7944
                     /gene="thiM"
                     /locus_tag="OO471_002913"
     CDS             7156..7944
                     /gene="thiM"
                     /locus_tag="OO471_002913"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6244104.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7941..8741
                     /gene="thiD"
                     /locus_tag="OO471_002914"
     CDS             7941..8741
                     /gene="thiD"
                     /locus_tag="OO471_002914"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6244105.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8806..9627
                     /locus_tag="OO471_002915"
     CDS             8806..9627
                     /locus_tag="OO471_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6244106.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9675..10421
                     /locus_tag="OO471_002916"
     CDS             9675..10421
                     /locus_tag="OO471_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6244107.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10395..11360)
                     /locus_tag="OO471_002917"
     CDS             complement(10395..11360)
                     /locus_tag="OO471_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6244108.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11357..12360)
                     /locus_tag="OO471_002918"
                     /pseudo
     CDS             complement(11357..12360)
                     /locus_tag="OO471_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12400..13452
                     /gene="fbaB"
                     /locus_tag="OO471_002919"
     CDS             12400..13452
                     /gene="fbaB"
                     /locus_tag="OO471_002919"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6244109.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13760..14614
                     /gene="gatY"
                     /locus_tag="OO471_002920"
     CDS             13760..14614
                     /gene="gatY"
                     /locus_tag="OO471_002920"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6244110.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14643..15905
                     /gene="gatZ"
                     /locus_tag="OO471_002921"
     CDS             14643..15905
                     /gene="gatZ"
                     /locus_tag="OO471_002921"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6244111.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15915..16367
                     /gene="gatA"
                     /locus_tag="OO471_002922"
     CDS             15915..16367
                     /gene="gatA"
                     /locus_tag="OO471_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6244112.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16398..16682
                     /gene="gatB"
                     /locus_tag="OO471_002923"
     CDS             16398..16682
                     /gene="gatB"
                     /locus_tag="OO471_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6244113.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16686..18041
                     /locus_tag="OO471_002924"
     CDS             16686..18041
                     /locus_tag="OO471_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6244114.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4726 a         4110 c         4573 g         4662 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggccgtag ccatattgaa ggggcgagca agcaaagtga ttttgtccag gcaggttatc
     2641 agtatggttt taataactta ttgacgctgt atggcggcac gatggttgct aataattact
     2701 atgcgtttac cctcggaacg ggttggaaca cgcgcattgg cgcaatttcc gtcgatgcca
     2761 cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt tatcaaattg
     2821 cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc tggcgttatt
     2881 cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa gataattatc
     2941 gccgtgatga aaacgatatc tatgacattg ccgattatta ccagaacgat tttggccgta
     3001 aaaatagctt ttctgccaat atgagccagt cattgccaga aggctggggt tctgtgtcct
     3061 taagtacgtt atggcgagat tactgggggc gtagcggcag cagtaaggat tatcagttga
     3121 gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag gcgtatggcg
     3181 agaatcatca tgaagagaaa cgttttaata tttttatatc aattccctgt gattggggtg
     3241 atgacgttac gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg tttgatgatc
     3301 agggttttgc ctcaaataat acgggattat caggaacagt agggagtcgg gatcagttca
     3361 attatggtgt caacctgagt catcaacatc agggaaatga aacgacagct ggggcgaatt
     3421 tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg agtacttatc
     3481 gacagactgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg
     3541 cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa gatgcttatg
     3601 tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtggtatac gacggaatga
     3661 caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat
     3721 tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg gttaattttg
     3781 ataccgatca gcgcaagccc tggtttataa aagcgttaag agcggatggt caaccattaa
     3841 cgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc ggccagggaa
     3901 gccagttatt tattcgcacc aatgaaatac cgccatcggt taatgtagca attgataagc
     3961 aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt agaaattata
     4021 tttgccagta atagtcaggt ggttctatgg aaattcgcat aatgctattt atattaatga
     4081 tgatggttat gcctgtgagc tatgcggcat gttatagtga gttatctgtt cagcacaatt
     4141 tggttgttca ggggaatttt gcacttactc aaacacaaat agcgacatat gagcataatt
     4201 ttaatgattc gtcatgcgtg agtacaaata ctatcacccc tatgagccca tcggatatta
     4261 ttgttggact ttataacgat accattaaat taaatttaca ttttgaatgg accaataaaa
     4321 acaacatcac gttgtcaaat aatcagagca gtttcaccag tggttattcg gtaacggtga
     4381 cacctgcggc cagtaatgca aaagtgaata tttctgcggg gggcggtggt tcagtgatga
     4441 ttaatggtgt tgcgacatta tccagtgctt catcatcgac acgcgggagt gccgcagtac
     4501 aatttctact gtgtttatta ggtggcaagt catggggtgc atgtgtaaat agctacagaa
     4561 atgcattggc acaaaatgca ggtgtctatt ccttcaatct gacattgtca tacaacccga
     4621 taaccacaac ctgcaaacag gacgatttat taattacttt agacagtatt cccgttttac
     4681 aattaccagc cacaggtaac aaagcaacaa taaatagtaa aaaaagggat attattctgc
     4741 gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag gtgtatttat
     4801 caagttctga cttgttaacc aacagcaaca caatactgaa aggtgcggaa gataatgcgt
     4861 aggatttatt cttgaaagta atggttcgcc agtcacactt ttaaatatca ctaacagcag
     4921 taaaggatac acaaatttaa aagaaattgc ggcaaagtca aaacttacag atacaacggt
     4981 ttcaattccg ataacagcca gttactacgt ctacgataca aacaaagtta aatctggcgc
     5041 actggaggca accgcattaa tcaacgtgaa atacgactaa taacaagcaa aacgagcaag
     5101 tggctaataa taaaaaataa caaggtgtct gcatttcccc ctcatgatga gggggctttt
     5161 tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc
     5221 tgatcaacac gtacgcgcca cccatatagg tccagtgcgt cccggcatca ggcgcgggca
     5281 ggttacgtaa ctgccactgc ttaatgttgt actcatcggt gcggtacatc tctgggacgg
     5341 tatcacctgg tttgaaatgc ttaaagtcag caatgaaact acttaactca tacttagaaa
     5401 ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca
     5461 gcaaaagcac acccagcaac atcttatttt taatggtcat aaactctcca ctttggggat
     5521 ctgtaatatc agtcgcatat ctttcctgtc atacaatata actgacaagc gatattttgt
     5581 tagtgatccc atgatttcgt aaaaagtgta aatgaaagga tgccctaaaa gcatccttag
     5641 cgagcagaga gtcagcggaa atagctgatt taagaatcat cctgggctga agataacatg
     5701 caagcctaaa ggattgagtt atcgcattat gcccatgaag ccatgtacgc ccatatacac
     5761 accgactaag ccaatcaaca gactggaaaa atagggggcg cgtttagcga gagtgttaaa
     5821 tccgctccag cgttttgcga cctgctgaac gctgattgct gcgccaacgc ctacggtgac
     5881 aagcgttaac gccaggccaa tgctgaaact gacgaccagt gttgcgccca gtgtcagggc
     5941 tttcaactga atgcatatca acagcacggt aattgctgcc gggcagggga taaagccacc
     6001 ggttaagcca aataacaaaa tttgccagtt gatgacctct ctaccatcaa agcgtcgttt
     6061 aatgtcattg gcatgggctc gtgcatgggc atcctgatac tcgccatgtt cgtgatggtg
     6121 atgatgtcct tggtcgtggt gatgttcgtg atcgtgatga tggtgatgat gctcataatc
     6181 atgcccgtgc atattctcca gccagttgcg ttcgccgcgc cagaacatcc agaacgcggt
     6241 gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt gagcagtaaa
     6301 gcgcttactg atcaccatcc cgccaaaggc aatcaaccag accaccgcgg tatgcgaaat
     6361 ggttgctgcc agtccgagca tcaccgcctg tttaatggtg cctttgatgg cgatgataaa
     6421 cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac caagtaagat
     6481 ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa attcggtcat
     6541 gataataatt cttagtatta attcggcaat ctgattctac tcccccccag tacctgatac
     6601 taccccccag tagattaata gtgctatgat ttttcatatt cttgttaacc aggtgttgcc
     6661 atgtctcata caatccgtga taaacagaaa ctgaaagcgc gtgccagtaa gattcagggc
     6721 caggtcgtgg cgctcaagaa aatgctcgac gagccgcacg aatgcgctgc agttttacaa
     6781 cagattgctg ctatccgtgg agcggtaaac ggtctgatgc gggaagtgat taaaggtcat
     6841 ctgacggaac acatcgttca ccagggggat gagctaaaac gtgaagaaga tctggatgtc
     6901 gttctgaagg tgctggattc atatatcaaa taatttatta acgcgattgt aaaactgccg
     6961 tttttcctcg tttacaacgg gggcgctgga cattaccatc ctcctctgcg atttatcatc
     7021 gcaaccaaac gactcggggt gcccttctgc gtgaaggctg agaaataccc gtatcacctg
     7081 atctggataa tgccagcgta gggaagtcac ggaccaccag gtcattgctt cttcacgtta
     7141 tggcaggagc aaactatgca agtcgacctg ctgagttcag cgcaatctgc gcacgcgtta
     7201 cacctttttc accaacattc ccctcttgtg cactgcatga ccaatgatgt ggtgcaaacc
     7261 tttaccgcca ataccttgct ggcgctcggt gcatcgccag cgatggttat cgaaaccgaa
     7321 gaggccagtc agtttgcggc tatcgccagt gccttgttga ttaacgttgg cacactgacg
     7381 cagccacgcg cacaggcgat gcgtgctgcc gttgagcaag caaaaagctc tcaaacacgc
     7441 tggacgcttg atccagtagc ggttggtgcg ctcgattatc gccgccattt ttgtcatgaa
     7501 cttttatctt ttaaaccggc agcgatacgt ggtaatgctt cggaaatcat ggcattagct
     7561 ggcattgcta atggcggacg gggagtggat accactgacg ccgcagctaa cgcgataccc
     7621 gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg tggtcactgg cgaggtggat
     7681 tatgttaccg atggacatcg tatcattggt attcacggtg gcgatccgtt aatgaccaaa
     7741 gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg cctgctgtgc gttaccaggc
     7801 gatacgctgg aaaatgtcgc atctgcctgt cactggatga aacaagccgg agaacgcgca
     7861 gtcgccagaa gcgaggggcc aggcagtttt gttccacatt tccttgatgc gctctggcaa
     7921 ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct gacgattgcc ggtactgatc
     7981 cgagtggtgg tgcggggatt caggccgatc ttaaaacctt ctcggcactt ggcgcttatg
     8041 gttgctcagt tattactgca ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc
     8101 gcattgagcc tgattttgtc gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg
     8161 ataccactaa aatcggtatg ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt
     8221 tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt tatgctggca aaaagcggcg
     8281 acccgctgct ttcaccttcg gcggttgcta cgctgcgcag tcgattattg ccacaggttt
     8341 cattaataac gccaaacttg cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca
     8401 ccgaacagga aatgctggaa caagggcgat cgctgttggc gatgggctgt ggcgcagtgc
     8461 taatgaaagg tggtcatctg gatgatgagc aaagcccgga ctggctgttt acccgcgagg
     8521 gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa cacccacggc actggttgta
     8581 cactctctgc ggcgttggct gcactacgcc cgcgccatac aaactgggct gacaccgtac
     8641 aggaggcaaa aatctggctt tcatcggcgt tagcccaggc cgacacgctg gaagttggtc
     8701 acggtattgg tccggttcac cacttccacg cctggtggtg agcaccgtac tatagctgct
     8761 ttggttattg tcagcctttg gcactgacat tcagggatga aaccaatgca gctaagaatt
     8821 accagtcgaa aaaaactcac cgctctatta tgcgctcttg ggctaatttc catcgttgca
     8881 atttatccgc gtcagacggt gaattttttc tattcgacag cagttcagat tacagactac
     8941 atccacttct acggttatcg cccggttaaa tctttcgcta ttcgcattcc tgccagttac
     9001 accattcacg gattagatgt ttcgcgctgg caggagcgta tcgactggca gcgggtggca
     9061 aaaatgcgcg acaacgatat ccgcttacag tttgctttta ttaaggctac ggaaggcgaa
     9121 aagctggtgg acccctattt ttcgcgtaac tggcaattaa gccgcgaaaa tggcctgctg
     9181 cgcggggcgt atcattattt ctccccgtcg gtatccgctt cagttcaggc gcgattattt
     9241 ctgcaaacgg tggatttctc acaaggcgat ttccctgccg tgctggacgt agaagaacgg
     9301 ggaaaattat cggcaaaaga attacgcaag cgggtaagtc agtggctaaa aatggtcgaa
     9361 aaacgcacgg gaaaaaagcc gattatttac tcaggagccg ttttttatca caccaatctg
     9421 gcgggctatt tcaatgaata tccgtggtgg gtggctcact attatcaacg tcgtccggac
     9481 aatgacggca tggcctggcg cttctggcag cattccgacc gtggacaggt agatggcatc
     9541 aatggtccgg tggattttaa tgtgtttaat ggcacgggga tgagttgcag gcattcgttg
     9601 atgggattaa agaaacgcct taaataagaa gcagtgatat actcccacca gactccctgg
     9661 caggaaaaag acaaatggaa caagcgcata cccagctgat tgcccaactg aacgagcgta
     9721 ttttagcggc ggataacacg ccgctttata ttaagtttgc cgaaacggta aaaaatgccg
     9781 tgcgcagcgg ggtgctggag catggcaata ttttgcccgg tgagcgtgat ttaagtcagt
     9841 taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca ggcgctggaa gaagagggcg
     9901 tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa catcttcgaa tactcgttga
     9961 aagaagcgag aggtttttct cagcaggtgg tattgcgcgg gaaaaagccc gatacgctat
    10021 gggttaataa gcgtgtcgtg aaatgtcccg aagaagttgc gcagcagctg gcggtcgaag
    10081 cgggaagtga tgtctttttg cttaagcgta ttcgctatgt cgatgaagaa gcggtatcga
    10141 ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt tgatgccatc ggaatttctc
    10201 tttatgacta tttccgcagc cagcacattt acccacaacg tacgcgttcc cgcgttagcg
    10261 cccggatgcc ggatgccgag tttcagtcac atattcagtt agatagcaaa ataccggtgc
    10321 tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc tattgagtac agcattagcc
    10381 actgtcgcag cgatctatac gtttttgtgt gcgaggagta gttcctcgcg cgttggcgca
    10441 caatcaccgc cccgatgccc gacaacccac gacgccactg cattgcccag taatacggca
    10501 tccgccagtg gcagaccaga ggccagcccg gcaagtacgc cacctgcatg actgtcgccc
    10561 gccccaatgg tgtctacaac ttgcgttgga aatgccggaa tgcagccaga agcgtcgttg
    10621 ctgaaatacc atgcgccttc tttatcgaga cgaacgatca acggcgcggc aaatttctcc
    10681 tgccattgct tgccaagtgt tgttatctct gcggataaag caaaacgttc ggcggcaatc
    10741 tcagcctctt gacgattgag tgacactaaa ggtcgacagg ccatgatccg cgccagtaat
    10801 gcgtccggga tatcgccaat acgtgggcaa aaatcgataa acggcgtcac gtcttgcagc
    10861 ttttccagcc attccactaa caattcgccg cagggcgagg ccagttgata accggaaaaa
    10921 tagagcaggt tgccaggcgc aacggttaat cgtgccaacc attggcgatt ccattgattt
    10981 tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg gctcaaccag tgccagacac
    11041 cagccgttat cgctttcggc gttatcgatc aggctgatta acccctcttt cgccatccgg
    11101 ttgcgaataa tctccgccca cacgccctga ccgagcggca aggcattacc cgcttcgatg
    11161 ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc cgccaacgtt aacgctctgc
    11221 tgtttcagtt cgatatcgca cccacgccag gggagggcat aagcgtcggc gatcacgtca
    11281 atgaccgccg cgccgacgac catcaccggc tgacgcgtgg ttaattcagg cagcagtgtg
    11341 tgtaatcgag cgccgctcat accgcctccc gttgttgacg atatttcgcc agcgctgtgg
    11401 catagcggtt gaaatcaagc tgatttaccg catccagctc cgcctttaac gcaggatcga
    11461 cagcgttaat gccatgcaac gcgccgcaaa tcgccgtcgc catagcacct atggtgtctg
    11521 tgtcgccgcc aagattggcg cacaggacgg cgcagcggtt tgggtcggtt tgtgccagtt
    11581 caactagcgc aatggcgcac ggaacggact caatagtgct ggtgcctgcg ccaacgacct
    11641 ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt acgcacaatt ttcagcgcaa
    11701 tctccagacg tgccgccagt gaggcgctga acgtggtggt gcgtttttgc tgtgcatgtc
    11761 gcgcaattga aggcaatgaa tcaacaatcg ctgaccagcg ttctccgtca atggcacgag
    11821 aaatcgccca tgcgatgact accgcgcctg caaccgccag atcggattta tgggtcgggc
    11881 tggacgccag cgccacatca tcaataaagg aatcaacatc acgcgctgga agcaaacaac
    11941 ctaatggcga gacgcgcatc gctgcgccgt tggttacgcc gttgttttcc agttcagcaa
    12001 cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct agtacgtttt
    12061 tgttaaaggc gtcgaaacgc aacgcccagt cgagaatatt acgcccaatc agattcggat
    12121 cgatcttgcc ttcacattcc agtaacgcat ccgccagaca caggccatcg aggtatcgtc
    12181 ggtgaattcg gcgcggttaa aataacaggc cgcgttattc tcctttggtc caggcagaaa
    12241 acggtcaatc cagccaaagt gtgctttaac gcggctgcgt ggccaaagct cggagggcat
    12301 ccccatcgca tcccctaacg cctgcccata aagagcaccg agaatacgtt ctgttttcat
    12361 ttaacttccc cttgaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg
    12421 cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc ttctgaccag
    12481 ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa tcgcccgcca
    12541 gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat
    12601 ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct
    12661 aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac
    12721 tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc gcatcgcatt
    12781 ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa
    12841 acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt tggtgcgact
    12901 atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcctttgaa
    12961 cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa ctccgccttt
    13021 aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc gaaccatctg
    13081 gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat
    13141 aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac cagcgaaaac
    13201 ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc tgggttgata
    13261 aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt
    13321 atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg
    13381 atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt
    13441 actattgcct gatacgcctc atacgaacag caccaccccg taggacggat aagacgttta
    13501 cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag
    13561 cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    13621 ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat attgttgttt
    13681 ttgcgatcat tatctcgaca tttaaaagca aataatttca ttatattttg aaatcgaaag
    13741 caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca
    13801 gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt
    13861 ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt
    13921 tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca
    13981 ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt
    14041 tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat
    14101 ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaggcgga
    14161 gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg atgcgttgta
    14221 caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt
    14281 cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt tttctcgact
    14341 ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt caggattatc
    14401 gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga
    14461 gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc ccgaagcgac
    14521 cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt
    14581 gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa
    14641 acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct
    14701 gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca
    14761 aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga
    14821 caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg
    14881 aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa gaaaatgcgg
    14941 atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta
    15001 aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta gcaccagaaa
    15061 cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc
    15121 agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc ggtgaggcca
    15181 gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc
    15241 atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg attgccatcg
    15301 tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag
    15361 cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca cattctaccg
    15421 attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag
    15481 tcggtcacgc attaaccttt gctttacgcg aggcgatatt tgcactggca caaattgagc
    15541 aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc
    15601 tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat tcattactgg
    15661 atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt cggattaaaa
    15721 atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg ggcatgatta
    15781 gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca gcaatgccgc
    15841 atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac ggctgtgcgg
    15901 aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt gtcgatcgta
    15961 gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt catgatacct
    16021 ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg cttgagcagc
    16081 acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc atttatctgt
    16141 taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac gtggcggtat
    16201 cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt ttacgctgtt
    16261 tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt cctgaaactc
    16321 agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc cctctgtaac
    16381 aataaaaagg actatttatg aaacgcaaga ttattgtcgc ttgcggaggc gcggttgcga
    16441 cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat attcctgttg
    16501 aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg catttgatat
    16561 gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac ggcatgcctt
    16621 ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc ttacagggat
    16681 gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca gtgatgctgc
    16741 cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat tgctttaaag
    16801 cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc ttaatgctgg
    16861 attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat ctgcatgtgg
    16921 tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt gcgctggtgg
    16981 cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg acgcgggtgg
    17041 taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg ctgcacctgg
    17101 caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg tttgtttata
    17161 agctcggcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
    17221 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
    17281 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
    17341 gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
    17401 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gttatgctgc
    17461 tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggccc
    17521 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg gatcccgcat
    17581 tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg ctcaccattt
    17641 taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt gccactatcg
    17701 gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc accttaatct
    17761 cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt ggtttgcaca
    17821 cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
    17881 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
    17941 gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg cgtagagcgc
    18001 gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct gagggggagg
    18061 ttatccccct t
//
LOCUS       DAJTUM010000080        18053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTUM010000080 DAJTUM010000000
VERSION     DAJTUM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            446..1129
                     /locus_tag="OO471_002925"
     CDS             446..1129
                     /locus_tag="OO471_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6244115.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO471_002926"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO471_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO471_002927"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO471_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6244116.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO471_002928"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO471_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6244117.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO471_002929"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO471_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6244118.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO471_002930"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO471_002930"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6244119.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO471_002931"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO471_002931"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6244120.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO471_002932"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO471_002932"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6244121.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO471_002933"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO471_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6244122.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO471_002934"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO471_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6244123.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO471_002935"
     CDS             complement(13386..13817)
                     /locus_tag="OO471_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6244124.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO471_002936"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO471_002936"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6244125.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO471_002937"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO471_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6244126.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO471_002938"
     CDS             16903..17862
                     /locus_tag="OO471_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6244127.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18053)
                     /locus_tag="OO471_002939"
                     /pseudo
     CDS             complement(17849..18053)
                     /locus_tag="OO471_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4548 a         4665 c         4674 g         4166 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg cac
//
LOCUS       DAJTUM010000081        17644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTUM010000081 DAJTUM010000000
VERSION     DAJTUM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO471_002940"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO471_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO471_002941"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO471_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6244128.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO471_002942"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO471_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO471_002943"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO471_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6244129.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO471_002944"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO471_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6244130.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OO471_002945"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OO471_002945"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6244131.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OO471_002946"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OO471_002946"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6244132.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO471_002947"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO471_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6244133.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO471_002948"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO471_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6244134.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO471_002949"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO471_002949"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6244135.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..9471)
                     /gene="astB"
                     /locus_tag="OO471_002950"
                     /pseudo
     CDS             complement(8138..9471)
                     /gene="astB"
                     /locus_tag="OO471_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9468..10946)
                     /gene="astD"
                     /locus_tag="OO471_002951"
     CDS             complement(9468..10946)
                     /gene="astD"
                     /locus_tag="OO471_002951"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6244136.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10943..11977)
                     /gene="astA"
                     /locus_tag="OO471_002952"
     CDS             complement(10943..11977)
                     /gene="astA"
                     /locus_tag="OO471_002952"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6244137.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11974..13194)
                     /gene="astC"
                     /locus_tag="OO471_002953"
                     /pseudo
     CDS             complement(11974..13194)
                     /gene="astC"
                     /locus_tag="OO471_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13640..14446
                     /gene="xthA"
                     /locus_tag="OO471_002954"
     CDS             13640..14446
                     /gene="xthA"
                     /locus_tag="OO471_002954"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6244138.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14613..15323
                     /locus_tag="OO471_002955"
     CDS             14613..15323
                     /locus_tag="OO471_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6244139.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15328..16005
                     /gene="ydjY"
                     /locus_tag="OO471_002956"
     CDS             15328..16005
                     /gene="ydjY"
                     /locus_tag="OO471_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6244140.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16020..16727
                     /locus_tag="OO471_002957"
     CDS             16020..16727
                     /locus_tag="OO471_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6244141.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16727..17275
                     /locus_tag="OO471_002958"
     CDS             16727..17275
                     /locus_tag="OO471_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6244142.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17285..17611
                     /locus_tag="OO471_002959"
                     /pseudo
     CDS             17285..17611
                     /locus_tag="OO471_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4366 a         4685 c         4307 g         4286 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cattaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca ccgcctcgct
     8821 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
     8881 cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac gattgtggtt
     8941 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga cgctaaattt
     9001 ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt ccagcgaacg
     9061 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
     9121 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
     9181 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
     9241 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcggaatca cggcctgggg
     9301 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg ccagtcgcgg
     9361 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
     9421 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
     9481 tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga gtccgactcc
     9541 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
     9601 ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag cggtttgttc
     9661 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
     9721 gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat cgcctcatca
     9781 aaagtatcat aacgccagac gcgcaaaaac ggcccgaaca cctcttcatc cggcacaccg
     9841 ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc ttgtaataag
     9901 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
     9961 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    10021 ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc atcgccctgc
    10081 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    10141 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    10201 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    10261 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    10321 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcggc
    10381 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    10441 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    10501 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    10561 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    10621 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    10681 ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc cagcaatccg
    10741 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    10801 gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc attgccttgc
    10861 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    10921 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    10981 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    11041 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    11101 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    11161 gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    11221 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    11281 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    11341 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    11401 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    11461 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    11521 cgcgaaaagc cgccataaac ataaagcgcg atttcgatag caaatagccg ttgccctctt
    11581 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    11641 taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    11701 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atagcgcaaa
    11761 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcgcttt
    11821 tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    11881 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    11941 agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    12001 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    12061 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    12121 cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    12181 caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    12241 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    12301 gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat aggtggtgcc
    12361 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    12421 gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc cgtagtgcat
    12481 ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa aaatcagcaa
    12541 ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    12601 cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt cgtcaatcag
    12661 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    12721 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    12781 aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt cgtgagcgaa
    12841 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    12901 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    12961 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt cacgcagttc
    13021 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    13081 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    13141 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    13201 ctctacagta aataattcgt tatttgtatg ttaacaataa gtaatgtttg cgctgtaaat
    13261 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    13321 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    13381 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    13441 tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    13501 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    13561 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    13621 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    13681 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    13741 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    13801 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    13861 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    13921 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    13981 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    14041 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    14101 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    14161 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    14221 ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    14281 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    14341 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    14401 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    14461 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    14521 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    14581 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    14641 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    14701 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggttatagcc
    14761 tctatattct gttattcatc aatgcccccc tctttctgtt accaggaagc atactggtga
    14821 tcgctggagg aatagttttt ggcccgctct tagggacaca actctcatta attgccgcaa
    14881 cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat ttactgctga
    14941 aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg cgtaacggta
    15001 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    15061 cttacggatt caccaccatc gccttctggc cttataccct tatttcggca ctcacgaccc
    15121 tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    15181 tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc atccagctcg
    15241 caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc agcccactta
    15301 ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt attcagtgtc atggaaaaaa
    15361 ggactggctg cactctgctt actggctgtt gccgggctta gcggctgcga tcaaaaagag
    15421 aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct gcgcgtcgat
    15481 gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct caccgacgac
    15541 actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct gtttatggct
    15601 tatgcgcccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa
    15661 aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact ggatatttcg
    15721 gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt tgacagtaat
    15781 ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga gaagaaaacg
    15841 ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc aacatacact
    15901 tatggtgcgg tggaaaaaca cggcgaagtg aaattcaaag gcaatgcctc agttctcccg
    15961 gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca agataaagga
    16021 tgatgaagat acaatcgcgt aaaatctggt actaccgtat aaccctcatc atcctgttgt
    16081 tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg
    16141 ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag tcttacggcg
    16201 cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct gcaccgctac
    16261 ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg ggcggcttgc
    16321 tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt ttttatcgcc agagtgatgg
    16381 gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct tgacagtatg gacggctttt
    16441 tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt gtccctttcg
    16501 atccaatcag ctatgctgcc ggtctgactt caatacgttt tcgctcgttt tttatcgcca
    16561 ccgggcttgg tcaattaccg gcgactattg tttattcctg ggcaggcagc atgttaacag
    16621 gcggtacttt ctggtttgtc accgaactgt ttattctgtt tgccctgacc gtggtgattt
    16681 ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgactgatgg gtttaccgcc
    16741 gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc gccattacgg
    16801 cgaggtgcta agcccgattc gctggtgggg gcggatcccg tttatctttt atttggtgtc
    16861 aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc gatccggtag tgcgatcgct
    16921 tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata tcaccagtat
    16981 gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg gcagtggctg actggcggca
    17041 aagcccgctc tttagcgatg aagaacggct ggcgctggag tacgccgaag ccgcaagcgt
    17101 cacaccgcca acagtcgatg atgccctgcg tacccgactg gctgcgcatt ttgacgctca
    17161 ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac ctgtcggctc gttttaattc
    17221 tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt cttaaggagg
    17281 gaagatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctt tggcaacaca
    17341 tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg caaaccgtct ggtttaacgc
    17401 ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg agatgaaaac
    17461 ccattacgct ataaacctga agattgttcg tctcgcggat gccgcagacg cggtgaagcg
    17521 cattcagacc gaagctgctg ccggtcgtaa aacgggcggc tcggtggatc tgctctgggt
    17581 gaacggcgaa aacttccgca ccttaaaaga gtaatgccag tcagttaagc aactgactgg
    17641 catt
//
LOCUS       DAJTUM010000082        17160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTUM010000082 DAJTUM010000000
VERSION     DAJTUM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..185
                     /locus_tag="OO471_002960"
     CDS             <1..185
                     /locus_tag="OO471_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6244143.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="OO471_002961"
     CDS             complement(210..401)
                     /locus_tag="OO471_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6244144.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="OO471_002962"
     CDS             complement(506..742)
                     /locus_tag="OO471_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6244145.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO471_002963"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO471_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO471_002964"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO471_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO471_002965"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO471_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6244146.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="OO471_002966"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="OO471_002966"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6244147.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="OO471_002967"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="OO471_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6244148.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="OO471_002968"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="OO471_002968"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6244149.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="OO471_002969"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="OO471_002969"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6244150.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="OO471_002970"
     CDS             complement(10860..12233)
                     /locus_tag="OO471_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6244151.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="OO471_002971"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="OO471_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6244152.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="OO471_002972"
     CDS             complement(13344..13583)
                     /locus_tag="OO471_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244153.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="OO471_002973"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="OO471_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6244154.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="OO471_002974"
     CDS             13960..14247
                     /locus_tag="OO471_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6244155.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14917..15060
                     /locus_tag="OO471_002975"
     CDS             14917..15060
                     /locus_tag="OO471_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6244156.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15073..15282
                     /gene="cspE"
                     /locus_tag="OO471_002976"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15073..15282
                     /gene="cspE"
                     /locus_tag="OO471_002976"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6244157.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15448..16257
                     /gene="rlmA"
                     /locus_tag="OO471_002977"
     CDS             15448..16257
                     /gene="rlmA"
                     /locus_tag="OO471_002977"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6244158.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="OO471_002978"
     CDS             complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="OO471_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6244159.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4518 a         4149 c         4338 g         4155 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 attatttatt agcgaaacgt ttctctcttg atttttttgc tcactcatca atttttctta
    14521 ttttaaattt acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
    14581 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc agaaaatgtg
    14641 atattgcacg cactatgtaa taacttctcc ctctggcctg gaacaactga acttattgaa
    14701 ctatgttaga aactacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
    14761 atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
    14821 ttgtgcgtct gatcgagata tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat
    14881 aaaagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
    14941 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
    15001 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
    15061 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
    15121 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
    15181 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
    15241 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
    15301 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
    15361 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
    15421 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
    15481 ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac atcagtttga tatggcgaaa
    15541 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
    15601 gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
    15661 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
    15721 attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc
    15781 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
    15841 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
    15901 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
    15961 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
    16021 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
    16081 cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
    16141 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
    16201 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
    16261 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
    16321 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
    16381 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgaaggaaa
    16441 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
    16501 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
    16561 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
    16621 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
    16681 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
    16741 ggggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
    16801 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
    16861 cccccagcaa acatgcatac atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg
    16921 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca
    16981 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
    17041 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
    17101 ttcattgctt tattgataac gattttcatt tatgtttata tatcaatgaa acgttaaaat
//
LOCUS       DAJTUM010000083        17093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTUM010000083 DAJTUM010000000
VERSION     DAJTUM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..510
                     /locus_tag="OO471_002979"
     CDS             <1..510
                     /locus_tag="OO471_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6244160.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO471_002980"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO471_002980"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6244161.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="OO471_002981"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="OO471_002981"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6244162.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="OO471_002982"
     CDS             complement(2878..3075)
                     /locus_tag="OO471_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6244163.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO471_002983"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO471_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6244164.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO471_002984"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO471_002984"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6244165.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO471_002985"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO471_002985"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6244166.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO471_002986"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO471_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6244167.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO471_002987"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO471_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6244168.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO471_002988"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO471_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="OO471_002989"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="OO471_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6244169.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO471_002990"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO471_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6244170.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="OO471_002991"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="OO471_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6244171.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="OO471_002992"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="OO471_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6244172.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="OO471_002993"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="OO471_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6244173.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="OO471_002994"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="OO471_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3993 a         4865 c         4445 g         3790 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc cgcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcca ggcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta att
//
LOCUS       DAJTUM010000084        16977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTUM010000084 DAJTUM010000000
VERSION     DAJTUM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..364)
                     /locus_tag="OO471_002995"
     CDS             complement(<1..364)
                     /locus_tag="OO471_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6244174.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGV"
     gene            complement(544..2985)
                     /gene="rnr"
                     /locus_tag="OO471_002996"
     CDS             complement(544..2985)
                     /gene="rnr"
                     /locus_tag="OO471_002996"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6244175.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3024..3449)
                     /gene="nsrR"
                     /locus_tag="OO471_002997"
     CDS             complement(3024..3449)
                     /gene="nsrR"
                     /locus_tag="OO471_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6244176.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3654..4952)
                     /gene="purA"
                     /locus_tag="OO471_002998"
     CDS             complement(3654..4952)
                     /gene="purA"
                     /locus_tag="OO471_002998"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6244177.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5056..5253)
                     /locus_tag="OO471_002999"
     CDS             complement(5056..5253)
                     /locus_tag="OO471_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6244178.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5335..6339)
                     /gene="hflC"
                     /locus_tag="OO471_003000"
     CDS             complement(5335..6339)
                     /gene="hflC"
                     /locus_tag="OO471_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6244179.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6342..7601)
                     /gene="hflK"
                     /locus_tag="OO471_003001"
     CDS             complement(6342..7601)
                     /gene="hflK"
                     /locus_tag="OO471_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6244180.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7687..8967)
                     /gene="hflX"
                     /locus_tag="OO471_003002"
     CDS             complement(7687..8967)
                     /gene="hflX"
                     /locus_tag="OO471_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6244181.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9043..9351)
                     /gene="hfq"
                     /locus_tag="OO471_003003"
     CDS             complement(9043..9351)
                     /gene="hfq"
                     /locus_tag="OO471_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6244182.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9437..10387)
                     /gene="miaA"
                     /locus_tag="OO471_003004"
     CDS             complement(9437..10387)
                     /gene="miaA"
                     /locus_tag="OO471_003004"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6244183.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10380..12227)
                     /gene="mutL"
                     /locus_tag="OO471_003005"
     CDS             complement(10380..12227)
                     /gene="mutL"
                     /locus_tag="OO471_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6244184.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTMPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12237..13574)
                     /gene="amiB"
                     /locus_tag="OO471_003006"
     CDS             complement(12237..13574)
                     /gene="amiB"
                     /locus_tag="OO471_003006"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6244185.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13593..14054)
                     /gene="tsaE"
                     /locus_tag="OO471_003007"
     CDS             complement(13593..14054)
                     /gene="tsaE"
                     /locus_tag="OO471_003007"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6244186.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14026..15573)
                     /gene="nnr"
                     /locus_tag="OO471_003008"
     CDS             complement(14026..15573)
                     /gene="nnr"
                     /locus_tag="OO471_003008"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6244187.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15572..16711
                     /gene="queG"
                     /locus_tag="OO471_003009"
     CDS             15572..16711
                     /gene="queG"
                     /locus_tag="OO471_003009"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6244188.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16694..16747)
                     /locus_tag="OO471_003010"
     CDS             complement(16694..16747)
                     /locus_tag="OO471_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244189.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3850 a         4835 c         4270 g         4022 t
ORIGIN      
        1 gaacgcctgc ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc
       61 cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt
      121 cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc
      181 ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg
      241 cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa
      301 aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc
      361 tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg
      421 atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca
      481 tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta aagagggtat
      541 tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt
      601 ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt
      661 cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg
      721 acaacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt
      781 accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc
      841 catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct
      901 ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga
      961 agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc
     1021 agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca
     1081 cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca
     1141 gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt
     1201 gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag
     1261 gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa
     1321 gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg
     1381 cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg
     1441 ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga
     1501 ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt
     1561 ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg
     1621 cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa
     1681 cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac
     1741 tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag
     1801 atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct
     1861 catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc
     1921 gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac
     1981 ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc
     2041 ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca
     2101 taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc
     2161 gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc
     2221 ttcttccggc acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat
     2281 atacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc
     2341 gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag
     2401 ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat
     2461 atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta
     2521 gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg
     2581 acctttacgg tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt
     2641 catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta
     2701 gccatcacgg tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata
     2761 gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg
     2821 caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct
     2881 ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt
     2941 cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt
     3001 gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc
     3061 tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc
     3121 ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa
     3181 ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc
     3241 ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga
     3301 ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact
     3361 tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat
     3421 ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat
     3481 acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg
     3541 aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc
     3601 aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg
     3661 tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg
     3721 atcggcacac cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg
     3781 ccgctacgat ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc
     3841 ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc
     3901 ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta
     3961 gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc
     4021 agccagccgg tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag
     4081 aggaactcgc cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt
     4141 gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg
     4201 gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg
     4261 atatccagca gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc
     4321 tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc
     4381 gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg
     4441 aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg
     4501 tcgccaacac gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga
     4561 ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg
     4621 tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg
     4681 ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg
     4741 ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg
     4801 gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca
     4861 acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat
     4921 tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat
     4981 ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat
     5041 cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc
     5101 aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga
     5161 gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac
     5221 cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc
     5281 accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg
     5341 cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat
     5401 cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta
     5461 gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc
     5521 accacgcata atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc
     5581 ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac
     5641 cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt
     5701 gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt
     5761 gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc
     5821 gttatctgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc
     5881 gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag
     5941 gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg
     6001 cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt
     6061 gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt
     6121 gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa
     6181 atgcagacca ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa
     6241 acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac
     6301 cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg
     6361 tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac
     6421 gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg
     6481 ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac
     6541 ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac
     6601 actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat
     6661 tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc
     6721 ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact
     6781 tcgttggaat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc
     6841 gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc
     6901 agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg
     6961 ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact
     7021 ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac
     7081 agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc
     7141 tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc
     7201 ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg
     7261 aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg
     7321 gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc
     7381 gggcgcgggc cttgtgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga
     7441 ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg
     7501 cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg
     7561 tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt
     7621 tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct
     7681 acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc
     7741 aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc
     7801 tattgcctga agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa
     7861 tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat
     7921 ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc
     7981 aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat
     8041 ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg
     8101 cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt
     8161 ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc
     8221 aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc
     8281 aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt
     8341 accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg
     8401 ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg
     8461 attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat
     8521 cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg
     8581 cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat
     8641 atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg
     8701 ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac
     8761 agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt
     8821 acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa
     8881 ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg
     8941 ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac
     9001 gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9061 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9121 gccaccggcg ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc
     9181 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9241 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
     9301 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
     9361 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
     9421 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
     9481 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
     9541 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
     9601 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
     9661 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
     9721 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
     9781 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
     9841 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
     9901 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
     9961 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10021 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10081 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10141 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10201 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataaggacag
    10261 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    10321 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    10381 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    10441 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    10501 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    10561 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    10621 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    10681 gactggaaat caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt
    10741 tcttcgccag aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct
    10801 tcacccggcg tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt
    10861 gaaatgttgc catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    10921 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt
    10981 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11041 cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag
    11101 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11161 acgcggtttt ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc
    11221 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    11281 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc
    11341 gggtcgatct ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    11401 caggcctggc ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag
    11461 caatactgaa tttccgccag tgcgggcata gtgtgatttg gatcggccac ccagccgcgc
    11521 agggtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg
    11581 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    11641 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    11701 cggatgattt catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    11761 gccggggtgt tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc
    11821 ggtttaaccg tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt
    11881 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    11941 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12001 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12061 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12121 tcgaggctgt tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12181 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12241 tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc
    12301 ggcgcagatt gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct
    12361 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    12421 aaaccggttt cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg
    12481 tgttctggtc gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    12541 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    12601 aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac
    12661 agtttctcgt gctgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc
    12721 acccatacgg aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg
    12781 aggaaattgg cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa
    12841 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    12901 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    12961 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13021 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13081 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13141 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13201 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg
    13261 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    13321 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat catgcccgtt
    13381 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    13441 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    13501 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    13561 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    13621 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    13681 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    13741 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    13801 gcaaggcggt acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc
    13861 agcgtataag tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg
    13921 ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    13981 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14041 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14101 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14161 cgcaccgtgc gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag
    14221 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    14281 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    14341 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    14401 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat
    14461 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    14521 caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg
    14581 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    14641 catagtcagt tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc
    14701 actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat
    14761 acgaatagcc cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc
    14821 tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg
    14881 ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag
    14941 ttgtcctgta acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt
    15001 gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc
    15061 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15121 gctaatggat tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag
    15181 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15241 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa
    15301 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    15361 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    15421 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    15481 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    15541 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    15601 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    15661 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    15721 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc
    15781 cggtacgctg cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    15841 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    15901 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    15961 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16021 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16081 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16141 ggaaggatgc ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16201 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16261 gctcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    16321 gcttatctgc ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg
    16381 taagccgcta cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt
    16441 aaaagtcacg gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat
    16501 cgccgtggca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    16561 taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    16621 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    16681 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    16741 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    16801 ttgattttta acttatttat caataggtta aaaaaatacc attcaccgtg cgaagtttct
    16861 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    16921 acaacaaaaa ccagcaataa cgcatcgcgt cgctgtagat attttattga gagaaga
//
LOCUS       DAJTUM010000085        16392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTUM010000085 DAJTUM010000000
VERSION     DAJTUM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO471_003011"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO471_003011"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6244190.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO471_003012"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO471_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6244191.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="OO471_003013"
     CDS             2341..2556
                     /locus_tag="OO471_003013"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244192.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="OO471_003014"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="OO471_003014"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6244193.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /gene="galP"
                     /locus_tag="OO471_003015"
     CDS             4451..5845
                     /gene="galP"
                     /locus_tag="OO471_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6244194.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="OO471_003016"
     CDS             5922..6419
                     /locus_tag="OO471_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6244195.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="OO471_003017"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="OO471_003017"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6244196.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="OO471_003018"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="OO471_003018"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6244197.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="OO471_003019"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="OO471_003019"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6244198.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9104..9667
                     /locus_tag="OO471_003020"
     CDS             9104..9667
                     /locus_tag="OO471_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6244199.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="OO471_003021"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="OO471_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6244200.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="OO471_003022"
     CDS             complement(10259..11239)
                     /locus_tag="OO471_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6244201.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="OO471_003023"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="OO471_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6244202.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="OO471_003024"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="OO471_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6244203.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="OO471_003025"
     CDS             12542..12832
                     /locus_tag="OO471_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6244204.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="OO471_003026"
     CDS             12840..13433
                     /locus_tag="OO471_003026"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6244205.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="OO471_003027"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="OO471_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6244206.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14877..15840
                     /locus_tag="OO471_003028"
                     /pseudo
     CDS             14877..15840
                     /locus_tag="OO471_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15885..16388
                     /locus_tag="OO471_003029"
     CDS             15885..16388
                     /locus_tag="OO471_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6244207.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4061 a         4220 c         4124 g         3987 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctacgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgagtcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccaaggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg ccgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggac tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cggcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ctgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcccttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taactatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact tgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 tctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggtgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgagca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cttgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tatacaatgg tgctgttatt acgcatctgg atgcagtgtg
    12061 ctcattgtga tttttacacc cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgcc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtgtg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgactatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact atatcattca
    14701 ttgattataa atatgtacaa cctggcgcac aaatttaata tttaatttct gactttattt
    14761 caagttgtga taaacataga caagttgtca gacaggttaa ccaatttgtc gcacaaatca
    14821 ctgataagca caattagata tcaaccaatt tccatccttc aacagggagt ttttttatga
    14881 aaatgaggtc atttacccgc tcattagtct gcgcatcctt gctggctctg gttagcactg
    14941 gcgtaaacgc cgcagaaaaa gtggcgttaa agttagcaca caacctggaa agaagtcatg
    15001 ttgtacatca gagttttgaa gagctggcga aagaggtaaa acagctgtca aaaggtaata
    15061 tggtgatccg tatttatccc agcagccaga tgggtaatgc gcgagaaact atggaattgt
    15121 tgcaaaatgg cgcactggat atgaccaaag gttccgccag tgatctggag tcttttgata
    15181 atatttatgc tatctataat ttgccgttct tattaagttg tttttggtga agttggtaaa
    15241 gaaattatgg actcaacaaa agacaaaggc ttttttgctt tatcagctta tgtagcgggt
    15301 actcgcagtt tctatgcgaa aaaaccgatt acaaagccag aagatcttaa aggactgaaa
    15361 attcgtgttc agccaagtcc gaccaccatc aaaatgattg aactgatggg tggttcaccc
    15421 actccaattt catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca
    15481 gaaaataacg tgccttcctg gatgcagact cgacatattg aaattgccaa agttttttct
    15541 gaagatgaac acgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa
    15601 ttaaccccag aacagcaaca aatcctcgaa acagcggcga agaaatccga agcttatcaa
    15661 caaaaattgt gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatggggggt
    15721 gaaattgtaa aagtggataa agcgccattc cgtaaagccg tgcaacctct gtttgacgac
    15781 ttcaaaaaag atccaaaaca agctgctttg ttagagaaat tcgacaacgc tgctcaataa
    15841 tattccgggc cagcctctgg ccctgatttt ccaggatgct tacaatgata tttaaccgcc
    15901 tgaaacttgc ggtagaccgc gtcattgcca cgttttctgt cgccgtaatg cttgctctgg
    15961 ttgtatgcgt agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa
    16021 ccgatgagct ggcacgcttt ttgatgatct gggtaggatt attaggtgct gcatataccg
    16081 tcggcgcgca gcgacatctc tctattgatt tatttgctct cgccctgaat aagcgcaaac
    16141 aattacttct tagtatagtc attaacgttc tcattttagg ttttgccggt tcggtcattg
    16201 tgactggtgg cctgaaatta attgataaaa cactggcaac atcacaggta tctgccgcta
    16261 tgcaaatacc gatgggatac gtatatatca ttctgccatt aagtggagtg gtaatgatgt
    16321 tttatgcctt gtgttttatt aatcaaagca ttcaacaact gaaacaacct gcgcaggagg
    16381 ccagttaatg ga
//
LOCUS       DAJTUM010000086        15928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTUM010000086 DAJTUM010000000
VERSION     DAJTUM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            444..1502
                     /gene="citC"
                     /locus_tag="OO471_003030"
     CDS             444..1502
                     /gene="citC"
                     /locus_tag="OO471_003030"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6244208.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1517..1813
                     /gene="citD"
                     /locus_tag="OO471_003031"
     CDS             1517..1813
                     /gene="citD"
                     /locus_tag="OO471_003031"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6244209.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1810..2718
                     /gene="citE"
                     /locus_tag="OO471_003032"
     CDS             1810..2718
                     /gene="citE"
                     /locus_tag="OO471_003032"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6244210.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2729..4261
                     /gene="citF"
                     /locus_tag="OO471_003033"
     CDS             2729..4261
                     /gene="citF"
                     /locus_tag="OO471_003033"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6244211.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4265..4815
                     /gene="citX"
                     /locus_tag="OO471_003034"
                     /pseudo
     CDS             4265..4815
                     /gene="citX"
                     /locus_tag="OO471_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4790..5668
                     /gene="citG"
                     /locus_tag="OO471_003035"
     CDS             4790..5668
                     /gene="citG"
                     /locus_tag="OO471_003035"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6244212.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5719..7181
                     /gene="citT"
                     /locus_tag="OO471_003036"
                     /pseudo
     CDS             5719..7181
                     /gene="citT"
                     /locus_tag="OO471_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7295..8101
                     /gene="rna"
                     /locus_tag="OO471_003037"
     CDS             7295..8101
                     /gene="rna"
                     /locus_tag="OO471_003037"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6244213.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8330..8740
                     /gene="rnk"
                     /locus_tag="OO471_003038"
     CDS             8330..8740
                     /gene="rnk"
                     /locus_tag="OO471_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6244214.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8971..10209)
                     /locus_tag="OO471_003039"
     CDS             complement(8971..10209)
                     /locus_tag="OO471_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6244215.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10430..10858
                     /gene="uspG"
                     /locus_tag="OO471_003040"
     CDS             10430..10858
                     /gene="uspG"
                     /locus_tag="OO471_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6244216.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10979..12544)
                     /gene="ahpF"
                     /locus_tag="OO471_003041"
     CDS             complement(10979..12544)
                     /gene="ahpF"
                     /locus_tag="OO471_003041"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6244217.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12789..13352)
                     /gene="ahpC"
                     /locus_tag="OO471_003042"
     CDS             complement(12789..13352)
                     /gene="ahpC"
                     /locus_tag="OO471_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6244218.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13724..14470
                     /gene="dsbG"
                     /locus_tag="OO471_003043"
     CDS             13724..14470
                     /gene="dsbG"
                     /locus_tag="OO471_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6244219.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14680..15582
                     /locus_tag="OO471_003044"
     CDS             14680..15582
                     /locus_tag="OO471_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6244220.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15729..15928
                     /locus_tag="OO471_003045"
                     /pseudo
     CDS             15729..15928
                     /locus_tag="OO471_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3952 a         4119 c         3854 g         4003 t
ORIGIN      
        1 tacgcagcgg aaatgccagt ctttggaaaa atgcaaactg tttattctcg ttaagctgca
       61 acattgtttt ccttgcgggt tgtcccgagc ctctttgacg gatgtaatta aaatgttgaa
      121 tcaaaataca tagatattaa ttatgatcgt ggcaaatttt attcattaaa tcaattaaat
      181 cacttaaacc tgcgagtcat ttcgtaatta cattaattaa aaaacttaaa accattaaat
      241 aaataaaaaa ccactaactc tatgtgaaat aaatcaaaat ttcacgccga aatactcctt
      301 aggatgtata gcgaaaggag aaaaagatat acctcgatca cccccctttc tcccaagtga
      361 aaataaaagg ttatcagttt gcaacattga acaacattcg ttgcaaatcg ataacaacat
      421 gcaccttcag gatactattt attatgttcg gcaatgatat tttcacccgc gtaaaacgtt
      481 cagaaaataa aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg
      541 acaccacagt cgaagtattt attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg
      601 gaattgccgg aaatattatt aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac
      661 tggcgctgac attagccact gaactgataa acctcgccta tgagcggcac agcacgcatc
      721 tgtttattta taccaaaacc gaatacgagg cgctgttccg ccagtgcggt ttttccacgc
      781 tgacctgcgt acccggcgtg atggtgctga tggaaaacag cgccacgcga ctgaaacgct
      841 atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc attgtgatga
      901 acgccaatcc ctttacgaat ggtcaccgtt atctgattca acaagctgcg gcacagtgcg
      961 actggttgca tctgttttta gtcaaagaag attcttcacg cttcccctat gaagaccggc
     1021 tggatctggt gttaaaaggc accgccgata ttccacgcct gactgtgcat cgcggctcag
     1081 aatacatcat ctcccgcgct acgttccctt gctacttcat taaagaacag agcgtcatta
     1141 accattgtta caccgaaatt gatctgaaga ttttccgtca gtacctcgct cccgcgcttg
     1201 gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc
     1261 aggatatgcg ctactggctg gaaacgccga ctatctccgc agcgcccatc gaactggttg
     1321 aaattgagcg gctgcgttac caggagatgc cgatatccgc ttcccgggta cgtcaactgc
     1381 tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt
     1441 tgcagaacct gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag acccccgcat
     1501 gagaaacagg tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc ttgagtctgg
     1561 ggatgtgatg atacgcatcg ccccactcga tacgcaggat atcgacctgc aaatcaatag
     1621 cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc attctggacg ttctcgcccg
     1681 ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa ggcgcactgg actgcatttt
     1741 acatgcacga ctggaagccc tgctggcacg cgccagcggt atcccggctc tgccatggga
     1801 ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg ccgcagcatg
     1861 ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta cccggctgat
     1921 gccctgatgt ttgacctcga agactccgtg gcattacgtg aaaaagacac cgcccgccgc
     1981 atggtttatc acgcgctgca acatccgctg tatcgcgata ttgaaaccat tgtgcgtgtc
     2041 aacgcgctgg attccgaatg gggtgttaac gacctggaag ccgtcgttcg cggtggtgcg
     2101 gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg ttctggatat tgaaaaagag
     2161 atcctgcgta tagaaaaagc ctgtggtcgt gaacccggca gcaccggcct gctggcggcg
     2221 attgaatctc cgctgggcat tacccgcgca gtggaaatcg ctcacgcttc cgagcgtttg
     2281 atcggtatcg ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga acgctccccg
     2341 gaaggaactg aactgctgtt cgcacgctgt tccattttgc aggccgcgcg ctctgcgggt
     2401 attcaggcgt tcgataccgt ctattccgac gctaacaacg aagccggatt cctgcaagaa
     2461 gccgcccaca tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc gcgtcagatt
     2521 gatctgctgc acaacctcta cgcaccgacc cagaaagaag tggatcacgc ccaccgcgtc
     2581 gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct gaacggcaag
     2641 atggtggacg gtccggttat cgatcgcgcc cgtctggtgc tctcccgtgc agaactttcc
     2701 ggcatccgcg aagaataagg caatcaaaat gacgcagaaa attgaacaat ctcaacgaca
     2761 agaacgggta gcggcctgga atcgtcgcac tgaatgcgat cttgccgctt tccagaactc
     2821 accaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc aaactgtgcg ccaacctgga
     2881 agaagcgatt cgtcgctcag gtttacagga cggcatgacg gtttccttcc atcacgcttt
     2941 ccgtggcggt gacctgaccg tcaatatggt gatggacgtc atcgcgaaga tgggctttaa
     3001 aaacctgacc ctggcgtcca gctccctgag tgattgccat gcgccgctgg tagaacacat
     3061 tcgccagggc gtggttaccc gcatttatac ctctggccta cgcggtccac tggcggaaga
     3121 gatctcccgt ggtctgctgg cggaaccggt gcagatccac tctcacggcg gtcgtgtgca
     3181 tctggtacag agcggcgaac tgaatatcga cgtggctttc ctcggcgtcc cgtcctgtga
     3241 tgaattcggt aatgccaacg gctacaccgg taaagcctgc tgcggctccc tcggctatgc
     3301 aatggttgat gccgacaacg caaaacaggt cgtgatgctt accgaagaac tgctgcctta
     3361 tccgcataat ccggcaagca ttgagcaaga tcaggttgat ttgatcgtca aagttaatcg
     3421 cgttggcgat gctgcaaaaa tcggcgctgg tactacccgt atgaccacta acccgcgcga
     3481 actgcttatt gcccgtagcg ctgcggatgt gattgtcaac tctggctact tcaaagaagg
     3541 tttctccatg caaaccggca ccggcggcgc ttcgctggcg gtaacccgtt tcctggaaga
     3601 caaaatgcgt agccgcgata ttcgcgccga cttcgccctc ggcggtatta ccgcgacgat
     3661 ggttgatctg cacgaaaaag gtctgatccg caaactgctg gatgtgcaga gctttgacag
     3721 ccatgctgcg caatcgctgg cccgtaaccc caatcacatc gaaatcagcg ccaaccagta
     3781 cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc gacgtggtgg tactgagcgc
     3841 gctggaaatt gacaggcagt tcaacgttaa cgtgctgacc ggttctgacg gcgtactgcg
     3901 tggcgcttcc ggtggtcact gcgataccgc gattgcctct gcgctttcca tcatcgtcgc
     3961 gccactggta cgcggtcgta ttccgactct ggtggataac gtgctgacct gcatcacccc
     4021 gggctccagt gtcgatattc tggtcacaga ccacggtatc gcagttaacc cggcacgtcc
     4081 ggaactagca gaacgtctgc aggaagcggg cattaaagtg gtttccattg agtggctgcg
     4141 cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt gaattcaccg accgcgtcgt
     4201 tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt gtgcatcagg tgaaggaata
     4261 agccatgcac ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc ccgagctgct
     4321 cgtcagccgg gatgaaaggc aagcacggca acacgcctgg ctcaagcacc atcctgttcc
     4381 actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa gacagcgagg tcacacgccg
     4441 aatttttaat catcgcgtga cagccttgcg tgccttagcc acaaagcagg gctggcaaat
     4501 tcaggagcag gctgcactgg tttccgccag cgggccggag ggcatgttga gcattgccgc
     4561 cccggctcgc gacctcaagc tcgccaccat tgagcttgaa catagtcatc ctctcgggcg
     4621 gttatgggat atcgatgtcc tgacgcccga aggcgaaatt ctctcccgcc gcgactattc
     4681 actgcgcctc gccgctgcct gttgtgcgag caaagcgcag ccgtctgcgc gcgtggaaaa
     4741 acccatcaac tgaccgattt actcaaccgc atggaggcac tgctgaacga tgtcgatgcc
     4801 tgcaacgtca actaaaacca caaagcttgc gacgtcatta atcgatgagt acgccctgct
     4861 gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg aaaccaggcc tcgtggatcg
     4921 cattaactgc ggtgcgcaca aagatatggc gctggaagat ttccaccgca gcgcgctggc
     4981 gattcagggc tggttacccc gtttcattga atttggtgcc tgtagtgcag aaatggcacc
     5041 ggaagcggta ctccacggat tacgcccaat tggtatggct tgcgaaggtg atatgttccg
     5101 cgccactgcg ggcgtaaaca cgcataaagg cagcattttt tctttagggc tgctatgtgc
     5161 ggcaattggc cgtttgcttc agctcaacca gccggttacg ccaacaaccg tttgttctac
     5221 ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg cgtaccaata attcacgact
     5281 gacggcaggt caacggttgt accagcagct tggccttacc ggcgcacgcg gtgaagccga
     5341 agcaggttat ccactggtga tcaaccacgc cttgccgcat tacctcactc tgctggatca
     5401 ggggttagat cctgaactgg cattgctcga taccttgctc ctactgatgg ctaccaacgg
     5461 cgataccaac gttgcatcgc gcggtggcga ggggggcctg cgctggctac agcgcgaggc
     5521 gcaaacatta ttgcaaaaag ggggcattcg aacccccacc gatctcgatt atctccggca
     5581 gttcgacagg gagtgtatcg aacgaaatct cagtccaggc ggcagtgccg acctgctgat
     5641 ccttacctgt tttttagcac agatttaatt atttaagcac ttgataaatt tggaaatatt
     5701 aattttcgga gaacccgtat gtctttagca aaagataata tatggaaact attggcccca
     5761 ctggtggtga tgggtgtcat gtttcttatc cctgtccccg acggtatgcc gccgcaggca
     5821 tggcattact tcgctgtgtt tgtggcaatg attgtcggca tgatcctcga gccaattccg
     5881 gcaacagcga tcagttttat tgcggttact atttgcgtta ttggcagtaa ttacctgctc
     5941 tttgatgcca aagaattagc tgacccagcg tttaatgcgc aaaaacaggc gctgaaatgg
     6001 ggcctggctg gtttttccag caccacggta tggctggtat ttggcgcatt tatttttgca
     6061 ttagggtatg aagtttccgg gttaggtcgt cgcattgccc ttttcctggt gaaattcatg
     6121 gacaaacgca cgctgacgtt gggttatgcg attgtcatta tcgacattct gctggcaccg
     6181 tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt ttccggtcat taaaaacctg
     6241 ccgccgttgt ttaaatcatt cccgaacgat ccgtccgcgc gtcgtattgg cggctatttg
     6301 atgtggatga tggtcattag taccagtctg agttcgtcca tgtttgtcac cggtgcggac
     6361 caaacgtgct gggtctggag ttcgtcagca aaattgccgg tatccagatt agctggttgc
     6421 agtggttcct ctgcttcctg ccggttgggg ttatcttgct tatcattgcg ccgtggcttt
     6481 cctacgtgct gtacaaaccg gaaatcacac acagtgaaga agtggcaacc tgggcgggtg
     6541 atgaactaaa aaccatgggt gcgctgacac gcagagagtg gacgctgatt ggccttgtat
     6601 tgctcagctt aggtttgtgg gtatttggca gtgaagtcat taatgctact gcggttggtc
     6661 tgctggcagt ttcgctaatg ctggctctgc acgttgtgcc gtggaaagac attacccgct
     6721 ataacagcgc atggaacacg ctggtcaacc tggcaactct ggttgtgatg gctaacggcc
     6781 tgactcgttc tggttttatt gactggttcg ccggtaccat gagtacgcac ctggaaggat
     6841 tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt ctactttgca cactacctgt
     6901 ttgccagcct gtctgcgcac accgcaacca tgctgccggt tattctggcc gtcggtaaag
     6961 gtattccggg cgtaccaatg gaacaactgt gtatcctgct ggtgctgtct atcggtatca
     7021 tgggctgtct gacgccgtat gcaaccggtc ctggggtgat tatttacggc tgtggctatg
     7081 tgaaatcaaa agattactgg cgtcttggcg caatcttcgg ggtgatttac atctctatgt
     7141 tgctgttggt tggctggccg attctcgcca tgtggaacta atacgttcgc caaacgctga
     7201 ataaatcatc acgcccgcca ggtaagccca cctggcgggc ttttttatga tttaatagat
     7261 agtattcagt gtatgtatga gttccacacc cattatgaaa gcattctgcc gtaacgccgc
     7321 gttgctcgcg gtttctctgc ttcccttatc ttctgccaac gccgtagcgt tgcaggcaaa
     7381 acagtatggc gattttgatc gctatgtcct ggccctctcc tggcaaaccg gattttgcca
     7441 gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt cgcctgcaaa ccgaaacgac
     7501 caacaaaact gattttctga ccgtacatgg tctgtggcca ggattgccta aatcggttgc
     7561 tgcccgtggt gttgatgaac gccgctggat gcgcttcggt tgcgctactc gcccaatccc
     7621 gaatctacca gaagcacgcg ccagccgaat gtgttcatcg ccggaaaccg ggttatcact
     7681 ggaaacggcc gctaaactaa gtgaagtcat gccaggagct ggcggacgtt cctgcctgga
     7741 acgctacgaa tatgccaaac acggtgcctg ctttggtttt gatccggacg catacttcgg
     7801 tacgatggta cgcctgaatc aagaaattaa agagagcgaa gccggaaaat tccttgcgga
     7861 taattacggt aaaacagtga gccgccgtga ctttgacgcc gcctttgcca aaagctgggg
     7921 aaaagagaac gtgaaagcag ttaagctaac gtgccagggt aaccctgcgt atttgactga
     7981 aattcagatc tcgatcaaag ctgacgccat caacgctccg ctttctgcaa actcattctt
     8041 gccacaacct cacccaggta actgtggcaa aacctttgtg attgataaag cgggttatta
     8101 atcctctgat ttacaaaaca cgctaattcg tcttccataa ccatgccgat taagaattat
     8161 acataatgcg ctgcattgag gaattccctg aatgtgacgc aaatcacttc aaagagtgag
     8221 caacagtcag tatcatcatg tgagttaaac cctcgccgcc tgacggtgag ggttttcttt
     8281 tgggtatgtt tatctgcgac actcgcagta ccgacgacat ggagtaaaaa tgtccagacc
     8341 aactatcatc attaacgacc tggatgccga acgcatcgat attctgctgg agcaacccgc
     8401 ctatgctggt ttgccaatcg ccgacgcgtt aaacgcagag ttggatcgcg cccaaatgtg
     8461 ttcgccagaa gagatgccac acgacgtggt gacaatgaac agccgggtta aattccgcaa
     8521 tcttagcgat ggcgaagtgc gtgtgcgcac gctggtgtat ccggcaaaaa tgaccgatag
     8581 caatactcag ctttccgtta tggctccggt aggtgccgca ctgctggggc tgcgcgttgg
     8641 cgattccatt cactgggaac ttccgggcgg cgttgcaacc caccttgaag tgctggaact
     8701 cgaataccag ccagaagctg ctggcgacta cctgctttaa tcccatctgc acaagcgata
     8761 actcgctttg cccagaggat gcactcgcat cctctttacg caaaatcagc aatttttcct
     8821 ggttctcact gttcactttt gctgcaaaat tattccgttt cgtttgtggg aataaccatg
     8881 gcagaagcat tttacatcct gataggattt ctgattatgg cggcgattat cgtcatggct
     8941 gtcctttacc tcgaaaacca ctcctgacga tcatattgtt ccccccggca tcgcattcac
     9001 cagacctgaa accgccttct gcgccgcctc tgcgctttgt gcaccgggta ccagaatcac
     9061 cttacggcac tcctcttcac gtttttcgaa aatctcatat ccccgggcgg cctcttcaaa
     9121 cggcatatag tgggtaacaa tttcttctgg tttcagtaat cctttctcaa ttaacggtaa
     9181 taattctccc agccatgcgt gaacgtgggt ctgtcccatt ttaaacgtca accctttatc
     9241 aaaggcgtcg ccaaacagga aaccgtgaat aaatccagcg tagacgcccg gtacgctaac
     9301 aatgccgcca cgcctgaccg ccgcaataca ctggcgcaac gctttaccgc tgctgccctc
     9361 cagtttcagg ttagtcagca ccgtttccgt ggtgctgcct ttcgcttcaa aaccgacggc
     9421 gtctattact gcatccacgc cccggtgacc tgccgtttgt tcaataattg actgtgccgg
     9481 atcgctatct tcatcgaaat taatcgggat cgcgccgtag cggtcagcgg cgaaacgcaa
     9541 gcggtaggga tgatgatcaa caacaaaaat ctgttccgca ccgagcaacc gtgcacaggc
     9601 gattgtcaac aatcccacag gaccagcacc atagactgca acgcttgaac cttgttggat
     9661 ctgcacattt tttgctgcct gccatgccgt tggcagaata tcagaaagga aaagggcttt
     9721 atcatctgaa agcaaaggcg gtactttaaa cggccccaca ttccctttcg ggacgcggac
     9781 atattccgcc tgcccgccag gaacgccacc atacaggtga ctataaccaa acaatgccgc
     9841 gggagcaggt atctgttttt tattgagcgc agcgccttta cccgcattgg tattttcgca
     9901 ggcggcatat tgctgtaatc gacagaaaaa acagtcgcca caagcaatga cgaacggaat
     9961 taccacccgg tcgccttttt gcaaattttt tacgtccttc ccggtttcaa cgacttcccc
    10021 cataaattca tgaccaaaaa tatcgccatg tttaacctgg ggtattttgc ctcgataaag
    10081 atggaggtca gagccacaga tcgccgttac cgtaatacgc agaataatat catctgcctg
    10141 ttcaatgccc ggatcgggaa cattttctac ctgaacgtga tgtgggccgt gataagtcaa
    10201 tgctttcatg caacctccag agacagttca aaaaagagtc agtctttaag ggtagctatc
    10261 acagcgggaa aacgggcggt accgcaggtt ttgggagaat tcctagtgtt cagtgagaat
    10321 atattaatca cagaaaccgt tcgtaaggaa aaagaaccag acaaatatca gggataaata
    10381 ttataatcat ggagaatgat ttgttcatga ttaacaggga gaaagggtta tgtataaggc
    10441 aatcattatg ccagttgatg tatttgaaat ggaattgagc gacaaagctg ttcgccacgc
    10501 tgaattcctc gcccaggatg acggagttat tcatctactt cacgtactac ccgggtcagc
    10561 cagcctgagc ctgcaccgtt ttgccgctga tgtgcgtcgt tttgaagagc atctgcaaca
    10621 tgaagcagaa gaacgtctgc aaacgatggt cagccacttc accatcgatc cttcccgcat
    10681 taaacaacat gtccgttttg gtagcgtgcg ggatgaagtc aatgagttgg cagaagaact
    10741 gggggctgat gttgtagtta tcggttctcg caacccatcg atttcgaccc atctgttagg
    10801 ttctaacgcc tcgagcgtga tccgccacgc caatctgcct gtactggtcg ttcgctaaca
    10861 gataaaagta tttatgccgc cctgcctgga acatcccggc agggcttttt aatgccataa
    10921 aaaagccgcc aggtttgacc ccggcggcta agcaattgca ggtgaatctt acttcttctt
    10981 atgcagtttt ggtgcgaatc aggtagtcaa aagcactcag agaggctttg gcaccttcgc
    11041 cagtggcgat gatgatctgc ttgtacggaa ccgtcgtaca gtcacccgct gcgaacacgc
    11101 ctttgacgtt ggtttcacat ttcgcatcaa tgataatctc gcccatgcgg ttacgttcga
    11161 ctgcgccttc cagccagttg gtgttcggca gcagaccaat ctggacaaaa ataccggcca
    11221 gttcgatgtt gtgaatattg ccgctgacac gatctcgata ttccagacca acgactttgc
    11281 tgccgtcgcc tttcacttcc gtggtttgcg cattcagaat aatgtcgacg tttttcaggc
    11341 tgcgcagttt gtcctgcaga acctggtcgg ctttcatttc tggcgcaaat tccagcagcg
    11401 ttacgtgctc aacgataccc gccaggtcga ttgccgcttc cacgccggag ttaccgccgc
    11461 cgataaccgc tacgcgtttg cctttaaaca gcgggccgtc gcagtgcggg cagtaggtca
    11521 cgcctttagt gcgatactga tcttcgcccg gaacgttcat gttgcgccat tttgcacccg
    11581 tcgccacgat aatgctgcgt gctttcagta ccgcgccaga agctgtttca atctggtgta
    11641 gaccaccttc aaccgctgcc gggatcagtt tgctggcgct ctggctgtcg atcacatcaa
    11701 cgtcgtattc atcaacgtga actttcagtg cgcctgccaa cttctgccct tcagtcttcg
    11761 gtacagaaat gtagttttcg atatcaacgg tatcgaggat ctgaccacca aaacgttcgc
    11821 ccatcagacc ggtacggatg cctttacgtg cggagtaaat tgctgccgct gcacccgccg
    11881 ggccggaacc aacgattaat acgtcataag catcacgctt gttcagctct tctgccgcac
    11941 gattttccgc gccagtatca attttggcaa cgatttcagt caacgtcatg cggccctgac
    12001 caaactcttt cccgtttacg aacactgccg gaacgcccat cacgttgcga tcggtgattt
    12061 cgttctggaa ggtgccgccg tcaattgcag tgtgcttgat gcgcgggttc aatacgctca
    12121 tcaggttcag cgcctgcacc acgtccgggc agttatggca agagagcgag taataggttt
    12181 cgaattcaaa atcaccgtca atatggcgaa tctgctccag cagagactgc gcttctttcg
    12241 acggatgacc accggtccac agcaacgcca gtaccagcga ggtgaactcg tggcccagcg
    12301 gggagcctgc aaaacgtggt ccctggttgg aacctgggtt ggtgatcagg aaagacggct
    12361 tacgcactgg caagctgtta tcttctttaa aggtgacttt gtctgacagt tctgcgattt
    12421 cagccaacag ttccttgatt tctgccgatt tagcgctgtc atccagcgtg gcaattaact
    12481 caacaggctt ggtcaatttc tcaaggtaag ccttgagttg agttttcata tttgtgtcga
    12541 gcatgtttat ctcctgggct ttaaacatca tcatgcaagc ggcctctgcg gctacctgga
    12601 tgcagcttgc atcatggtgc ggggaagaaa atcgggtgcc gcagcacccg aagaattagg
    12661 tgaatttccg aagtatttcg cgtcagagca aggcgactag cctgtgaatc cccgggagct
    12721 tacacaagta agtgaccggg gtgagcgggc gacgccaacg ccgctatggc gtgaaagacg
    12781 aaggaaattt agattttacc aaccaggtcc agagacggag ccagagttgc ttcaccttct
    12841 ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgagc tgctttgatt
    12901 ttacgcagca gatcagacgc gtcacggcca atgccttcag cggtaacttc gattgcctgg
    12961 atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc atcttcacgc
    13021 atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc atatttgatt
    13081 ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt atcggtagat
    13141 actgcgtata cgtctacgcc cagtttctgc agttcttcgt agtggtcagc aacgtcaccc
    13201 agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac gctccagcgg
    13261 ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc ctggttttta
    13321 aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt ttcgatgaga
    13381 tgtaaggtaa ccaatttttg ccgattcggc taatgcgttt ccattatcaa atcgataagt
    13441 tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc ggcgataaaa
    13501 agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg gtatcttcag
    13561 cgcctgcctg caggctgagg tgttgccctt acaaatgcaa caacgacatg gattacaaca
    13621 ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacag tgataggtta
    13681 tgccttttac tcgacttttg cactgactga aaagaacaaa ttaatgttaa aaaagatact
    13741 tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttccttccc cagtaaaagc
    13801 gattgaaaaa cagggcatta caatcatcaa aacgttcgat gcccccggag gaatgaaagg
    13861 ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactcccg atggtaagca
    13921 cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca cacttatcga
    13981 aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac aatcccactg
    14041 gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttctccg atccgttctg
    14101 cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg gcaaagtgca
    14161 attaagaaca ttgttggttg gggttatcaa gccagaaagc ccggcgacag cagcggcaat
    14221 tcttgcctcc aaagatcccg caaaaacctg gcaacaatat gaagcctctg gttgcaagct
    14281 taagctaaac gtgcctgcaa acgtaagtac agagcaaatg aaagtgttaa gtgacaatga
    14341 gaaactgatg gacgatctgg gggcaaatgt cacgccggct atctattaca tgagtaagga
    14401 aaatacgctg caacaggccg tggggttgcc cgatcagaaa acgcttaata tcattatggg
    14461 aaataaataa aaggtaattc atggatggtt gaactatatc cctgcttttc aaccatccta
    14521 aaaaagcatg aatttctcgc tctaccctaa gtggctgtgc tattttagaa aaacttaatc
    14581 ctgagttaag caaaaaaaca taatcaataa aaatacatac gcaaaaaaca ttgattaagt
    14641 gaatatacca tggaagaaaa atataaaccg gagtagtgta tggccaatct ctacgacttg
    14701 aaaaagctcg acttaaatct tcttgtcata tttgagtgta tttaccagca tttaagcatt
    14761 agcaaagctg ctgaatcact atatatcacc ccatcagccg tgagccagtc gttacaacgc
    14821 ttacgtgcgc aatttaatga ccctttattc attcgctcag gaaaaggtat cgcgccgacg
    14881 acaacagggt tgaatctgca tcaccatctt gagaaaaacc tcaaaggcct tgaacaaaca
    14941 attaatatag tcaataagtc cgaactaaag aaaaacttta ttatctacgg ccctcagctc
    15001 atttcttgct ctaataatag tatgctgatc cgctgcctac ggcaagatgc ctctgtcgag
    15061 attgagtgcc atgatatcct catgtcagca gagaatgctg aagaactgtt ggttcaccgc
    15121 aaagccgatc tggtgatcac ccaaatgcca gtgatcagcc gttccgtcat ttgcatgcca
    15181 ttgcatacaa ttcgcaatac tctaatttgt agtaataagc atccgcgaat tactgacaat
    15241 agcacttatg aacaaattat ggcagaagag ttcacccaat taatagccaa atcagcagga
    15301 gttgatgata ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt
    15361 ggttcctctt tgctgacaat aattaatagc attgcagtta cagatttact gggaatagtc
    15421 ccctatgaac tttataacta ccaccgtgac tttcttaatt taaaagagat aaaactagaa
    15481 catccgttac cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg
    15541 gtgttctccc gatttattga ccgtctaaat gagagttttt gatatataac tatagacagc
    15601 tactataact ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttac
    15661 agtgtttact ttataattaa cagtcttatc tcactgttca ccctgtaacg ttcagagagc
    15721 gtcttcagat gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag
    15781 agcgtattac ctagacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca
    15841 aagactctgg tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa
    15901 tctgcgtttt gtttatcaac tgggaggc
//
LOCUS       DAJTUM010000087        15602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTUM010000087 DAJTUM010000000
VERSION     DAJTUM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..73)
                     /locus_tag="OO471_003046"
     CDS             complement(<1..73)
                     /locus_tag="OO471_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR6244221.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO471_003047"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO471_003047"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6244222.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO471_003048"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO471_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6244223.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO471_003049"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO471_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6244224.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO471_003050"
     CDS             2703..2939
                     /locus_tag="OO471_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6244225.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO471_003051"
     CDS             2942..3799
                     /locus_tag="OO471_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6244226.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO471_003052"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO471_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO471_003053"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO471_003053"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6244227.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO471_003054"
     CDS             complement(6414..7310)
                     /locus_tag="OO471_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6244228.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO471_003055"
     CDS             complement(7359..7598)
                     /locus_tag="OO471_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6244229.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO471_003056"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO471_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6244230.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO471_003057"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO471_003057"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6244231.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO471_003058"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO471_003058"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6244232.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO471_003059"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO471_003059"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6244233.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO471_003060"
     CDS             10765..12879
                     /locus_tag="OO471_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6244234.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO471_003061"
     CDS             12889..14181
                     /locus_tag="OO471_003061"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244235.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO471_003062"
     CDS             15131..15223
                     /locus_tag="OO471_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6244236.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>15602)
                     /locus_tag="OO471_003063"
     CDS             complement(15267..>15602)
                     /locus_tag="OO471_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6244237.1"
                     /translation="KSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCA
                     CCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQE
                     PTEMIQATA"
BASE COUNT         3577 a         4131 c         4096 g         3798 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tt
//
LOCUS       DAJTUM010000088        15526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTUM010000088 DAJTUM010000000
VERSION     DAJTUM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO471_003064"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO471_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6244238.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO471_003065"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO471_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6244239.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO471_003066"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO471_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO471_003067"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO471_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6244240.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO471_003068"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO471_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6244241.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO471_003069"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO471_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6244242.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO471_003070"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO471_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6244243.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO471_003071"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO471_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6244244.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO471_003072"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO471_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6244245.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO471_003073"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO471_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6244246.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO471_003074"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO471_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6244247.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO471_003075"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO471_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6244248.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO471_003076"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO471_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6244249.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO471_003077"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO471_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6244250.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO471_003078"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO471_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6244251.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO471_003079"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO471_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6244252.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO471_003080"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO471_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6244253.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO471_003081"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO471_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6244254.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO471_003082"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO471_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6244255.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO471_003083"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO471_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6244256.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO471_003084"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO471_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6244257.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO471_003085"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO471_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6244258.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO471_003086"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO471_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6244259.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO471_003087"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO471_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6244260.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO471_003088"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO471_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6244261.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO471_003089"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO471_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6244262.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO471_003090"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO471_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6244263.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO471_003091"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO471_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6244264.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO471_003092"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO471_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6244265.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO471_003093"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO471_003093"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6244266.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO471_003094"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO471_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6244267.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3643 c         4130 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTUM010000089        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTUM010000089 DAJTUM010000000
VERSION     DAJTUM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO471_003095"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO471_003095"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6244268.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO471_003096"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO471_003096"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6244269.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO471_003097"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO471_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6244270.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO471_003098"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO471_003098"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6244271.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO471_003099"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO471_003099"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6244272.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO471_003100"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO471_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6244273.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO471_003101"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO471_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6244274.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO471_003102"
     CDS             complement(8065..9207)
                     /locus_tag="OO471_003102"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6244275.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO471_003103"
     CDS             complement(9216..10229)
                     /locus_tag="OO471_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6244276.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO471_003104"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO471_003104"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6244277.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO471_003105"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO471_003105"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6244278.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO471_003106"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO471_003106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6244279.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO471_003107"
     CDS             complement(12827..13951)
                     /locus_tag="OO471_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6244280.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO471_003108"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO471_003108"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6244281.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4381 a         3446 c         3144 g         4373 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccgata
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgg cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcaac aagaattggc gggtaaattg ctatttaagt caaa
//
LOCUS       DAJTUM010000090        14978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTUM010000090 DAJTUM010000000
VERSION     DAJTUM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(520..1461)
                     /gene="fabY"
                     /locus_tag="OO471_003109"
                     /gene_synonym="yiiD"
     CDS             complement(520..1461)
                     /gene="fabY"
                     /locus_tag="OO471_003109"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6244282.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="OO471_003110"
     CDS             complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="OO471_003110"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6244283.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1940..2812)
                     /locus_tag="OO471_003111"
     CDS             complement(1940..2812)
                     /locus_tag="OO471_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6244284.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="OO471_003112"
     CDS             complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="OO471_003112"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6244285.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3504..4289)
                     /locus_tag="OO471_003113"
     CDS             complement(3504..4289)
                     /locus_tag="OO471_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6244286.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="OO471_003114"
     CDS             complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="OO471_003114"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6244287.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5387..6283
                     /gene="yihU"
                     /locus_tag="OO471_003115"
     CDS             5387..6283
                     /gene="yihU"
                     /locus_tag="OO471_003115"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6244288.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6307..7185
                     /gene="yihT"
                     /locus_tag="OO471_003116"
     CDS             6307..7185
                     /gene="yihT"
                     /locus_tag="OO471_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6244289.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7202..8443
                     /gene="yihS"
                     /locus_tag="OO471_003117"
     CDS             7202..8443
                     /gene="yihS"
                     /locus_tag="OO471_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6244290.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8581..9483
                     /locus_tag="OO471_003118"
     CDS             8581..9483
                     /locus_tag="OO471_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6244291.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9682..11718
                     /gene="yihQ"
                     /locus_tag="OO471_003119"
     CDS             9682..11718
                     /gene="yihQ"
                     /locus_tag="OO471_003119"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6244292.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11764..13138
                     /locus_tag="OO471_003120"
                     /pseudo
     CDS             11764..13138
                     /locus_tag="OO471_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13181..14584
                     /locus_tag="OO471_003121"
     CDS             13181..14584
                     /locus_tag="OO471_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6244293.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14652..>14978
                     /gene="ompL"
                     /locus_tag="OO471_003122"
     CDS             14652..>14978
                     /gene="ompL"
                     /locus_tag="OO471_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6244294.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3674 a         3856 c         3735 g         3713 t
ORIGIN      
        1 tgatttaata gcgtttgctt cggtgattta ttactcatta gttgtttttc taaattaaca
       61 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      121 cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt aaatgaatga
      181 tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta tagattttaa
      241 aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa atcgctcaaa
      301 cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag ctctacaaat
      361 aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca acgaagaata
      421 aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat gtaaatgacc
      481 ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt cgttcccgcc
      541 ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc
      601 acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg
      661 cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat ggggtttacc
      721 gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg
      781 gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca gcgagaataa
      841 actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca tggtggtgat
      901 aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa gcgggatatg
      961 ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa tatcatccag
     1021 agtggcgacg ggcttaatca tcaaaaaatg gcgaatcggc gtggtggttg gcgtggtgat
     1081 ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt cacgggcgct
     1141 acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca tcgccatcag
     1201 cgttcctaac cctttgtcct gcacgtccgg gtgaacgacc ataaagcgaa tggacgcttc
     1261 attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct cgtcgacgac
     1321 catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg gttgatgcag
     1381 gggcttacgc aacatttccc agcgaaagtg atagtaacgc tctaactctt cttctgtttg
     1441 tggaacctga aggtgataca tagcgatact ctctcttgtt tcccgtgaca accctggaag
     1501 ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga tacctgcata
     1561 tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg ttcgacgaaa
     1621 tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact tgggcgcatc
     1681 ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact gccgcccgcc
     1741 tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta gccgagcaca
     1801 cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc caataacacc
     1861 aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct ggcacgggtt
     1921 acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct gcttgtttta
     1981 gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg atacaccatg
     2041 tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa atgagctgat
     2101 atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg aacaggagtg
     2161 cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc
     2221 tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc agcacgttgt
     2281 cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa ctgatcgcca
     2341 gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg gcgaacgagt
     2401 aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta tcgatggagt
     2461 acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc atcttgttgg
     2521 aattagcaac aaattgttcg atataccgct gaataacatc gccagtagca ggcagaaagt
     2581 tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga aaagcggcga
     2641 aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca taggcaaggt
     2701 tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt tttagccagg
     2761 cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc ataacacctt
     2821 cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa tgcctagctg
     2881 attagctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa
     2941 accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca tccccagatc
     3001 ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt ccggccagaa
     3061 ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct gctcacgcag
     3121 tttatgcatg atggcgatca cttccgggcg cagcgcaaca aacaccgcct gccagccgtg
     3181 agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct ctgcgaacgc
     3241 ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat gaaaactctt
     3301 cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca cacggttaaa
     3361 gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg cctcctttcg
     3421 cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc aaggtatgaa
     3481 gccagattaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat ttcaatttgg
     3541 ctgtcttgta agcgtgattt tagcgcagcg ggcggcagct cgtcggtaaa gagcgcagtg
     3601 acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt gtgatcggcg
     3661 accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc ttcgtttaca
     3721 tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac cagataatca
     3781 gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct attatgagag
     3841 cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag aatatgcgcc
     3901 acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc ccgggcaaca
     3961 tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat atagtctgcc
     4021 accgcttcgg caatcgcttt ttttttctcg gtttgcgaaa cctcacgctg ctcgaacgcc
     4081 gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc
     4141 tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag cagatttgcc
     4201 agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc
     4261 gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat caccctggca
     4321 ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat cccggctcgt
     4381 ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac tgactccgct
     4441 aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc tcccgcacct
     4501 gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc attttccagc
     4561 cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag cgtttgtgcc
     4621 tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc cggttctgaa
     4681 aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt aatgtccccg
     4741 tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt agcgccgtcg
     4801 tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc ctccaaccac
     4861 tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc gcctttggta
     4921 tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt gtaacgggtg
     4981 ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgcgtcgtc atcacctacg
     5041 cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc cgtcgccgct
     5101 ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc cgtcggtaac
     5161 ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac acgaatcatg
     5221 gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt ttaaaaagtg
     5281 acggctgtca attttttgac gataaattac aaattcgatc aaaaacagac aagcaaagca
     5341 tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg cagcaatcgc
     5401 gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc agcaagggca
     5461 tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag acaaaggtgc
     5521 gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca ttaccatgtt
     5581 gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt gcgaaggctt
     5641 atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa ccgataaatt
     5701 gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag gccgtacttc
     5761 tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg aacaagttga
     5821 acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaacgctg gcggtccggg
     5881 catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca atgcgctttc
     5941 ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt
     6001 gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa acaaagtcct
     6061 cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc ttggcatcgc
     6121 ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt
     6181 ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca
     6241 ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg ataaaggaat
     6301 aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg ttttgccatg
     6361 ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg ggcacccgct
     6421 ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc cctctcgcct
     6481 tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt tgagcaaaac
     6541 gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc tggcaacggt
     6601 attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat caaacaagac
     6661 ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc gcagcaacgt
     6721 ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt aagcatcatt
     6781 gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca agcgatcatc
     6841 gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga aatgccgctt
     6901 tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa tgaccatatc
     6961 aatatgccat gggtgatcct ctcttccagt gtcgacgaaa aactgttccc gcgtgccgta
     7021 cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt ctgggcatcg
     7081 gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg tttgcgcacc gaaattacaa
     7141 caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga ggataaaagg
     7201 aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa ccgaccgcat
     7261 ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag gcaataaagg
     7321 gcaaatcaaa gaagagatgg gcacccatct gtggatcaca gctcgtatgt tgcacgttta
     7381 ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg ttggttgatc acggtattaa
     7441 agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct gcgtgaatga
     7501 cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc tgctgggtgc
     7561 cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt acaccattga
     7621 gattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggagt cctgggacga
     7681 agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg cggtggaagc
     7741 tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg cgattcgcgt
     7801 ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta acgaacactt
     7861 cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc atcgcttccg
     7921 cgcgttcgga ggtacaccag gccactggat cgaatggggc cgtttaatgc tgcacatcca
     7981 tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag atgccaaagg
     8041 tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggagcggacg ggattgttta
     8101 taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc ctatcgtcga
     8161 agcaatgggt acggcctacg cgctctacac cgtcactggc gatcgccagt atgaaacctg
     8221 gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa atggttcctg
     8281 gtggcaggag ctggatgcgg acaataaggt catcaccaaa gtctgggacg gcaaacagga
     8341 tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc ccggcatggc
     8401 tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct gaaaataggg
     8461 tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccggtgca
     8521 gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa ggtgccttgt
     8581 atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc gggggattat
     8641 cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca gggttgtcat
     8701 ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa agtgcttatt
     8761 ccgtggccga accggatcgc caacggctgt taccgttatc aggggcagga atatcagtta
     8821 cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg gcgagactgg
     8881 caaattagtg aattaaccac caccagcgtt accctgacag cttttctgcc gcccagttac
     8941 ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca cacgggtctt
     9001 tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg cgtgggtatt
     9061 catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca gcttccggca
     9121 aaccaggttt atgccgtcga tgaacacgta aatccgacaa cgcttcacca cgttgatgag
     9181 ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga ccacaccttt
     9241 aaaaccgcta atgatttgtg ggaaatgacc attacccatc cacagcaggc gctttcggta
     9301 agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt acagcgtcag
     9361 ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg tatcgactta
     9421 ttattactgg aaccaggaaa accgcaccgc ctgtttttta atatttacgg acagagaaaa
     9481 taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct aaaggcaaca
     9541 ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt ttttatatat
     9601 cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac aatctcgttc
     9661 tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga ttttcaattt
     9721 catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt aacccataac
     9781 aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat gttccgcggt
     9841 aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc catcgtcagc
     9901 cagtcaccgg atggttggtt aattcatttc agccgtggtt ctgacattag cgccacgctg
     9961 aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga caaccttaac
    10021 cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta cggctgcggc
    10081 gaacagtttt cctacttcga tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa
    10141 ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa agaaaatgcg
    10201 ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac gcagaagtat
    10261 tactgccatg ttgataacag ttgctacatg aacttcgact ttagcgcccc ggaataccat
    10321 gaactggcgc tgtgggaaga caaagctacg ctacgttttg aatgtgctga cacatacatc
    10381 tccctgctgg aaaaattaac tgccctgctg ggacgccagc cagaactgcc cgactggatt
    10441 tatgacgggg taacgctcgg cattcagggc gggacggaag tgtgccagaa gaaactggac
    10501 accatgcgta acgcgggcgt gaaggttaac ggcatctggg cgcaggactg gtctgggatc
    10561 cgtataacct cttttggcaa gcgcgtgatg tggaactgga agtggaacag cgaaaactac
    10621 ccgcaactgg attcacgcat taagcagtgg tatcaggagg gcgtgcagtt cctggcctat
    10681 atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca acacggctat
    10741 ctagcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt ttacggcggc
    10801 gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat caaaaagaac
    10861 atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct gcccaccgac
    10921 acgtacctgc ataacggcgt cagtgccgaa attatgcata acgcctggcc tgcgctgtgg
    10981 gcgaagtgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat cctcttcttt
    11041 atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc gggcgaccag
    11101 aacgtggact ggagtctcga cgatggcctg gcgtccgtag tcccggcggc gctgtcgctg
    11161 gcaatgaccg gacatggtct gcatcacagc gacattggcg gttacaccac cctgtttgag
    11221 atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg
    11281 atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg cgatgcggaa
    11341 actatcgccc attttgcccg tatgactacc gttttcacca ccctgaaacc ttatctgaaa
    11401 gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca tgcgcccgct gttcctgcat
    11461 tacgaagacg atgcacatac ctattccctg aaatatcagt acctgttagg ccgcgacatt
    11521 ctggtcgctc cggtacacga agaaggtcgc agcgactgga cgctctatct gccggaggat
    11581 aactgggtac atgcctggac gggtgaagcg ttccggggcg gggaagttac cgttaatgcg
    11641 cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc actgttcgcg
    11701 tcgttaaaca gcatctaatc acatccgccc gtggggaaac tcatgggcaa agggagaaca
    11761 tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt taaagagaaa
    11821 ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg tacgctttat
    11881 cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg cattatcttt
    11941 ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat gttggattcc
    12001 cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc gtcattcccg
    12061 gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg
    12121 gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat gaactgctcc
    12181 tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg aacgcgcctc actggcggca
    12241 tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt cgtgccagtt
    12301 atgaatctta tcgaaggtaa tcagaaactt ggctatatct tcgccgccac gctgttttca
    12361 ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg ttatgtcgaa
    12421 acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg cgcaattgcc
    12481 ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg ggcgtttaac
    12541 gtcaagctcg ccattcaggt ctattacacc cagtacgtac ttaacgatcc catcctgttg
    12601 tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct gatgcctggc
    12661 gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg
    12721 ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc gttctcctgc
    12781 ctggcgttct tcggctcagc gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg
    12841 cgagtggcgc accggcgtgc gttcggaagg cacggtctac accggcttca ccttctttcg
    12901 caaagtgtct caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaattggcta
    12961 tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg ttacgccagt tgatcttcat
    13021 ctacccaagc gcactggcgg tagtcaccat tgtggcgatg ggttgcttct acagcctgaa
    13081 cgagaagatg tatgtccgca ttgtggaaga gatagaagcc cgtaaacgca cggcgtaata
    13141 ataataaacg acgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact
    13201 gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg tcggttcgaa
    13261 tttaatgctc tgcatcggta ctctgtatct cctcaaactt tataccgatg aattagggat
    13321 gcctgcttac tatggcggga ttatctttct ggtcgcgaag ttttttaccg cgtttaccga
    13381 tatgctcacc ggatttttac tcgactcgcg gaaaaatatt gggcctaaag gcaaatttcg
    13441 tccctttatt ttatatgcag cagttccggc ggcgttaatt gcaacgctcc agtttatcgc
    13501 caccaccttt tgtttgccgg ttaaaacgac gattgccacc acgctgttta tgatgtttgg
    13561 actttcatac agtctgatga actgctcgta tggtgcgatg atcccggcaa ttaccaaaaa
    13621 cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt
    13681 gatttgtacc gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta
    13741 tgcctgtgcg gcacttatgt tctccattgg cggctttatt tttatgatgc tgtgctacag
    13801 aggcgtcaaa gagcattatg tggacacagc gccaaccgga cataaagcca gtattctcaa
    13861 atctttttgc gcgatatttc gtaatccacc attgctggtt ttatgcattg ctaacctgtg
    13921 tactctggcg gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct
    13981 gaatgatatt aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg
    14041 cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg
    14101 catggtgcta tgggcggtgg gtgatatact gaattatttc tggggaagta actctttcac
    14161 tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc tttgtaaaca gcctgaactg
    14221 ggcactggta ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg
    14281 ttctgtttat accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt
    14341 gccaggcatt atgctgacgc aaattggtta tgttcccaac atcgcgcaaa gcgatgcgac
    14401 attacagggt ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc
    14461 attgacgatg ggattctttt acacactcaa tgaaaaacgg tttgcattga ttattgagga
    14521 aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa aaaacggctt ccgtaacgtt
    14581 ataaatgtca atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa
    14641 atggacttat tatgaaaaag attaatgcaa taattttatt atcttctctg acctctgcct
    14701 cggtatttgc tggcgcatac gtcgaaaaca gggaggcata taatcttgcc tccgatcagg
    14761 gcgaggttat gctacgtgtg ggttataact tcgatatggg cgcgggtatt atgttaacca
    14821 atacctacac ctttcagcga gaagatgaac taaaacatgg ctataatgaa attgaaggtt
    14881 ggtatccttt attcaaacca accgataaat taaccatcca gcccggtggc ttaattaatg
    14941 ataagagtat cagttcgggt ggtgcagtgt atctggac
//
LOCUS       DAJTUM010000091        14532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTUM010000091 DAJTUM010000000
VERSION     DAJTUM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(113..379)
                     /locus_tag="OO471_003123"
     CDS             complement(113..379)
                     /locus_tag="OO471_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6244295.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO471_003124"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO471_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6244296.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO471_003125"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO471_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO471_003126"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO471_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6244297.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO471_003127"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO471_003127"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6244298.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEMK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO471_003128"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO471_003128"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6244299.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO471_003129"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO471_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6244300.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO471_003130"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO471_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO471_003131"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO471_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="OO471_003132"
     CDS             complement(12641..14095)
                     /locus_tag="OO471_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6244301.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14532)
                     /locus_tag="OO471_003133"
     CDS             complement(14092..>14532)
                     /locus_tag="OO471_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6244302.1"
                     /translation="QDEQGRFSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVS
                     LVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIP
                     IENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3873 a         3670 c         3430 g         3559 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa atgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaaccgccc
    14521 ctgttcgtcc tg
//
LOCUS       DAJTUM010000092        14422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTUM010000092 DAJTUM010000000
VERSION     DAJTUM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..133)
                     /locus_tag="OO471_003134"
     CDS             complement(<1..133)
                     /locus_tag="OO471_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6244303.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            316..459
                     /locus_tag="OO471_003135"
     CDS             316..459
                     /locus_tag="OO471_003135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244304.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OO471_003136"
     CDS             complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OO471_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6244305.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OO471_003137"
     CDS             complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OO471_003137"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6244306.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OO471_003138"
     CDS             complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OO471_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6244307.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OO471_003139"
     CDS             complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OO471_003139"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6244308.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7052..7660)
                     /locus_tag="OO471_003140"
     CDS             complement(7052..7660)
                     /locus_tag="OO471_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6244309.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7731..8816)
                     /locus_tag="OO471_003141"
     CDS             complement(7731..8816)
                     /locus_tag="OO471_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6244310.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8819..9181)
                     /locus_tag="OO471_003142"
     CDS             complement(8819..9181)
                     /locus_tag="OO471_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6244311.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9718..11631
                     /gene="mtlA"
                     /locus_tag="OO471_003143"
     CDS             9718..11631
                     /gene="mtlA"
                     /locus_tag="OO471_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6244312.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11726..12874
                     /gene="mtlD"
                     /locus_tag="OO471_003144"
     CDS             11726..12874
                     /gene="mtlD"
                     /locus_tag="OO471_003144"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6244313.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12874..13461
                     /gene="mtlR"
                     /locus_tag="OO471_003145"
     CDS             12874..13461
                     /gene="mtlR"
                     /locus_tag="OO471_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6244314.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13473..13682)
                     /locus_tag="OO471_003146"
     CDS             complement(13473..13682)
                     /locus_tag="OO471_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244315.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13966..14328
                     /locus_tag="OO471_003147"
     CDS             13966..14328
                     /locus_tag="OO471_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6244316.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3523 a         3842 c         3625 g         3432 t
ORIGIN      
        1 gaatggcgtc ggcatgagga tgattacgtt ttttagcaat caggtaggga atatcatgta
       61 aaataataat cccatagaat atcaccagaa atacaaaaat aagcactccc agtgcaaaat
      121 agtccaggaa catatttccc tcaaagaata taaaaaagaa caattaacgc atattatgaa
      181 atgccatgat gcagtgcaat ttccttattt atataacgac atattgtttt ttatatactt
      241 tctgaaatgt gctgtctggt ttttcaacag gacgaggttt atcatattga aatagtggaa
      301 ctattggcca aactaatgaa taactccagt aaaacatcca cagtacagat taagcgtatt
      361 aaaccttcaa ttatctaccg tttattgctg attggcctcg gatcaccaat ggtgatttac
      421 ggcctggttc gcccgctcac catcgaaacg cgagattaaa ctcctgacaa aacacgcccc
      481 agaaacgcac ttctgttggc gtagtctggg ttattgcgca cgtaggttta aaacgtaaat
      541 tgtaacaacg tgcgctttgt ttatgccgga tgcggcgtgt acgccttatc cggcctacgg
      601 tcaggttccc gtaggcatga taagacgcgt aagcgtcgca tcaggcaatg aatacccaat
      661 gcgaccagct tcttatatca gaacagcccc aacggtttat ccgagtagct caccagcagg
      721 cacttggttt gctggtaatg ctccagcatc atcttgtggg tttcgcgacc gatacctgat
      781 tgtttgtagc caccaaacgc agcatgtgcc gggtaagcgt gataacagtt ggtccacacg
      841 cgcccagcct gtatgccgcg ccccatctta taggccagat taccgttgcg gctccagacg
      901 cccgcgccca ggccatattg cgtatcgttc gccagctcca gcgcttcttc catcgttttg
      961 aaggtggtca ccgccagcac cgggccaaaa atctcctcct ggaacacccg catattgttc
     1021 tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt tcagttcacc ttccagcagc
     1081 ttgcgccgcc cgcctgtgag cacgtcagcg ccctcttttt taccgatatc aatgtagttg
     1141 aggatggttt ccagttgccc gtgagaaacc tgcgcgccca tttgcgtcac gctgtcgagc
     1201 gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc gttccataaa gcgttcgtag
     1261 attgactcct gcactaaggc acgactcgga caggtgcaaa cttcgccctg gttaaaggca
     1321 aacagtgcaa agccttccag cgctttatcg aaaaaggcat cttcttcatc catcacatca
     1381 gcaaagaaga tatttggcga cttaccgccc aactccagcg tcaccggaat aatgttttgc
     1441 gttgcgtatt gcataatttg ttggcccact tccgttgagc cggtaaacgc cactttggcg
     1501 atgcgtttcg aggtcgccag atattcgcca atttccccac ctgcgccatt gaccacgttc
     1561 accacgcccg gcggcagtaa atcaccgaca atttccatta gcagcagtac agaaagcggg
     1621 gtaagacgtg cgggtttcag caccacacag ttgcccgccg ccagcgcggg agccattttc
     1681 cagctcgcca tcagcagcgg gaagttccac gggataatct gccccaccac gcctaacggt
     1741 tcatggaaat gataggccac ggtttcgcta tcaacttcac tgatcccacc ttcctgcgcc
     1801 cgaatacacg aggcgaaata gcggaaatgg tcaatcgcca gcggtacatc cgcagcactg
     1861 gtttcgcgaa tgggtttgcc gttatcccag gtttcagctg tcgctaacag ctcgaggttt
     1921 tgttccattc gatcggcaat cttaaacaga atcgccgcgc gatcctgcac cgaggtgtgc
     1981 gcccatttat ctttcacttt gtgcgcagca tccagcgcca gatcgatgtc tcgtttgccc
     2041 gaagacgcca cttcgcacag cagctgcccg gtcaccggcg tcagattctg gtaatactca
     2101 ccatcggcag gggctaccca ttcaccgcca ataaagttgt cgtagcgggc ttttaacttg
     2161 atggggaaac catactcgcc gggcttaatc tgtgctgaag ggggattatt ggtcatgatc
     2221 gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac tggcgaaatc ttcgccagtc
     2281 ggtaacagct ttacgacagc tatcacgaat ttacgggcaa ggattacaga gatagttgaa
     2341 aaagcggctg acaatttgcc agccgttgtt ggaatgatga ttacattgcg gcgcgataaa
     2401 tcaccacaat ttcttcgtga gttgcctgga tcgggttagt aaagccacag gcatctttca
     2461 gggcattagt cgccagaacc gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg
     2521 ccgggatatc cactttcttc gccagttcac ggatggcgtt aatgcaggct tcagcacctt
     2581 ccgcgtcgtt tttacctgtc acgttcacgc ccattgcagc ggcacagtca cgcagacgtg
     2641 cagcggcgac tttgctgttg aatacctgaa catgcggcag cagaacggcg ttacatacac
     2701 cgtgtggcag gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca taacccagag
     2761 aagcattatt gaacgccata ccggcaagga actgggcata agccattgct tcacgcgctt
     2821 tcgcattact gccatcttca acggctaacg gcaggttttc ggcaatcatg gtcacggctt
     2881 tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga aacatacgct tcgatagcgt
     2941 gcgttaaggc atccataccc gttgcggcgg tcagtgactt cggcatacca atcatcagag
     3001 aggagtcatt gacagaaagc agcggagtca cgtgcttatc aacaatcgcc attttgttat
     3061 gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc agaggccgta cccgccgtgg
     3121 tattgatggc gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct tcgtaatcgc
     3181 gaatatcgcc gccattggct gccaccagcg caataccttt tgcgcagtcg tgtggagaac
     3241 caccgcctaa ggagatcacg ctatcgcaat tattttcttt aagtaatttc aaacctgcgg
     3301 cgacgttttc cgtggtgggg ttaggttggg tgccatcata aataacgcta aaaatattgc
     3361 gttcttccag tgctttttgc acatcacccg ccatacctaa tttcgttaac atattgtcag
     3421 tgacaattaa ggtacgggta aatccataat ctgccatcat attcattgca tcagtcaatg
     3481 aatcagcgcc gatgacattc acagaaggaa taaagaaagt tgaagatgcc ataacacact
     3541 cctgaaagtg taaaaaaggg tgggcagctt actgaggatt ttcattcgaa aacatgatca
     3601 ttattattaa ttatcaaaat taatctaata aaaagcacat tgtattcatt aaacaatgtg
     3661 ctttatttaa aatcataaga aatttgtatt aatttatttt aaaaattaat taatcatttc
     3721 cttatttttc cggaaataat aatgcgtcgc gcaataaatg atcatttcca cgacgacgcg
     3781 taaagccaat gcggtcaaaa tattccagaa tctgaattgc cagctttcgg cctacgccta
     3841 agcgatcgcg gaaatccgcc gcgcaggttg aaccacactc ctgatcgaga tcgcggatca
     3901 tattggcaaa ttcgacaatc cgatcgttac ggtaataacg atctttgacg atcgcggtaa
     3961 ttattccttg ctgcgccgcc tggcgtagcg tcaggcgcat tgcctgctcg tcggtacccg
     4021 tctcttttgc cagatcacgc acccaccacg gctcgtcacc aaacagcggc tctgcttttt
     4081 gccagatggc ctgctgctct tcgctgaagc ccgctttgtg atccggcaga tgcagccaac
     4141 cgtgatgact gaggatgtcg ccgctttcgc gcatcttttc aatcagcaac agtactagcg
     4201 cttcatcttc cattggcaac gccatacgtc gcagacgttc gcgcccaggc ccaggttcat
     4261 cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat tttccgttgc cagcgagcgg
     4321 caaccggcgc attcaacaag ctataaccag cctgaatata gccaggctgt tgcagcagtt
     4381 cgcgcatccc ttcgccgttg agctggcgcg cccaagcgaa atcaggaaga ttaaccgcgc
     4441 cgcgttgcag gtgaacggct aacgcttcgc tatcattctg tgccctggcg agggaagcca
     4501 gccattgcag atactctggt ttacgtttac cgcgacgcgg cgggttaagc atcacgacgc
     4561 gcgctccggc cagcgtgttg cgggcagaga tatcgcgcaa taccaggcgg tcgttatctg
     4621 ccagccataa cggggtgtcg aagaccagtt cagcaaggtt atcttccagc agtgaaacgc
     4681 gtcccgtgac gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg gtcagcggtg
     4741 catgggtttg aagctcgaca atcacccgtg tgaacggctc tggcggcaca tcggcaagca
     4801 gccagtcgcc acggttaatc tgctcttttt ccgcatcacc cgcgatgtca agcgcgatac
     4861 gctgcccggc attggcggtt tctgttggct ggttttgcgc atgcagcgca cgtacacgca
     4921 tcggtttatt tacaccagtc agccagagtg aatcgcctac cttcacttcc ccgcttaacg
     4981 ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa tgcgcggtca atcgcgaggc
     5041 ggaaactatg ttggctggcg tgctcgcgtt ccggcaactg aagcagatgc tcgcgcaggg
     5101 catcaattcc ccgaccttcg gttgctgcgg tgataaacag ttttgcctca gcaaaaccgt
     5161 attcccgcag aacctccttt acctggcgtt caacctcatc aacacgcgct tcgtccacgc
     5221 gatcggcttt ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc aaaatcgcca
     5281 gatgctcacg ggtctgtgcc atcacgccgt catcgcacgc caccaccaac agcgcgtgat
     5341 cgataccacc aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg ggaacgtcga
     5401 taaaaccagg cacgcgacca tccggctgcg gccagtaggc atagccgaga tcgatggtca
     5461 tgccgcgctt tttttcttcc ggcagacggt cagcatttac gccagtaatc gcctgcaata
     5521 aggttgtttt gccgtggtca acgtgtccgg cagtcgcaat aatcatttca acaacatctc
     5581 caaaaaccgt tgctcatctt caaggcagcg taaatccagc cacaatcgtc cgtcataaat
     5641 acgaccaatc accggcactg gcaattcacg ccagcgggcg gctaatgact caaggtggct
     5701 accgcgtcca tcatggggtg taaacgttaa tgccgcgctc ggcaggcgat caaccggcag
     5761 cgaaccactg ccaatctgcg aaagacatgg cataacctgt accgcaaact ccgcgccgta
     5821 atgtgcgaca aggggagcct gtaaacgttg tgcctggatt tgaatgacct ctgcgctgcg
     5881 ggtaagcagg cgcagggtcg gtaatttttc actcagagct tcagggtgta aataaagacg
     5941 caacgtggct tccagcgccg cgagggtcat tttatccgcg cgtaatgcac gcttcagcgg
     6001 gtggctttgc aggcgggcga tcatctcttt tttaccaacg ataatcccgg cctgcggccc
     6061 gcctaacaac ttgtcgccgg agaagctcac cagactgacg cccgccgcaa tcaactcctg
     6121 cggcattggc tctttcggca aaccgtactg gctaagatcg accagcgagc cactgcctaa
     6181 atcagtcacc actggaacat ccagctcttt gccgagcgcc accagttcag cttcgtctat
     6241 cgctttggtg aacccctgaa tgctgtagtt actggtatgt actttcatca acagtgcggt
     6301 attttcattc accgcctgac gataatcatt cgcgtgcgtg cggttggtgg tccctacttc
     6361 gtgtagggtg cagcctgcct gacgcataac atcgggaata cgaaacgcgc cgccaatctc
     6421 caccagttcg ccgcgagata ccaccacctc tttcccgctg gcagtggccg ccaacatcaa
     6481 taacaccgcc gccgcattgt tattgacgat acaggcatct tccgcccccg taatacggca
     6541 cagcagctgc gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca gatcgtactc
     6601 gagggtcact ggcgaacgca tagcctgcgc aacggcttcc accgcggctt ccgcctgtaa
     6661 agctcgccca aggttggtat gcagcacggt tcccgtcagg ttgatcaccg gacgcagcgc
     6721 gctctgcgcc tctttcgtca accgggcatc gacttcttgc gcccagtttt cacaccacgc
     6781 aggcagcgtc tggctgccac gaatcacttc tcgcgcttcg tcgagcatct gacgcaacaa
     6841 ttccaccacg cgggtgtgac cataagtatc acgcaaagaa aggaaggagc tatcgcgcaa
     6901 taagcgatca atagccggaa gttgactata gagggaacgc gtttcggttg tcataggaaa
     6961 cctggctgat caaggccctc tcacacggag aagggcgttt aacataacca cggattgtaa
     7021 cgtgagatgg gtcaggagga catatcgcgc atcaagcctt tggcggttcg gtgcgagcaa
     7081 aactttcgcg gctaaacagg ttttcgacca gtttgactaa gtgtgggcgg tcgacacacc
     7141 aacctggcgc aacgcggcgg aaattgagat atccgacagc acaggcaatg gcgatagtcg
     7201 ccagattgac cgtatcggtt ttgagtgtgc catcgaccag atatccttcc agcacatcca
     7261 gactgcggtt gattttctcc cgctggcgta acaattcatc ttcagactgc tgcgccgccg
     7321 gacgcgcctg ttcacgcacc gataccagcc cggcatccat aatgccatcc gccagcgcct
     7381 caattttgcg cacccgcaac gactccagcg gatcgcgcgg caacatcgcc ggagcgacat
     7441 tcattaactc aatatattca gcgatgatcg gcgaatcaaa ccagcattcg ccctcttcgg
     7501 tcaccagcac cggcactttc cctaacgggt taaattgcgc cacgccgtta tccgcgttat
     7561 agggcagttc attaataaat tcgaaagtta tgcccttttc taacaacaga ataaaaagtt
     7621 tgcgtacaaa cgggctggtg tagctaccga cgagtttcat gccgagtcct ttgtgcgagg
     7681 aaaaatatca gtatggctca ggctcacgca aaacacaggt gatataagtc tcaatgatcc
     7741 aaataaagat aatgcatcca actggtcatt cttagcagca ctttccgcat caccgaaaca
     7801 tggctgaaat ggtcggagta aaccgccacc tgggcgtaga tgccgtcggg taaggcatcg
     7861 atatcatcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc gggaacgacc
     7921 gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac aggtaaaata
     7981 ctagtcaatt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac ttccgcatca
     8041 tcgccaggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat ttgccgtttt
     8101 tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta tgtacctggg
     8161 cggatcagta cctgagtgac gtagccattg cttggtgcgc gaatgacagt ctgctcaaga
     8221 ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg ctcgccgttg
     8281 accatactgt cgagctgact ctggatctgc gcctgctccg ccaccgagcc tttcactaac
     8341 gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga gaacggattc
     8401 accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg gtcgcgctcc
     8461 gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag cgtctttata
     8521 ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg gtaacgaacc
     8581 gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt gtcagtgact
     8641 tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc cttttgcgcg
     8701 gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac cagaaacacg
     8761 cctcccagcg ccgccgtcgc cagcgccacg taagttaaaa caatcaatag atccataatt
     8821 agatctccgg gaacgtggaa tagtcagtct ttttctcggc ggcgagttga tgctccagcc
     8881 gggaaattcg gtcagaaagt gcggcgattt ccggttcagt cgctttctcc tgcgacgcaa
     8941 catgtgactg cataccccag ccacgctccg gttgatagag cgtcgcccag atccacagaa
     9001 acggccagat aacatgcaga gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg
     9061 catgggggtg attgcgcttt ttggcaatca gataagggat gtcatgtatc gcgatgattc
     9121 cataaaaaat caccagaaag acgaagatca gcactcccaa cgcgaaatag tttagaaaca
     9181 tatctgcctc ggattcacgt ttatcagtgt tgttttttgg ctggcagcca gaagggagtc
     9241 aggctgatat tttgacaata atccgggttc gcgattctcg ccataacacc aaagaataat
     9301 ttttagaggt gatgagttgc ttagttacat aacgattgta tgacgaaggc ataacatgct
     9361 gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt gattcagatc acaaagattc
     9421 aacaaaccat caaaacaaaa atgtgacatt actcacattt aaatgccatt tttagcgaaa
     9481 atcaccgcct tgtttctttt ttatacaggc gttttgtgat gagcgtcacg tcaattacct
     9541 ctctaccccc tatatttatg tgactgatat cacacaaaag cccgtcgact ggacagttaa
     9601 ccgattcagt gccagatttc gcagtatcta caaggtccgg ctacctctgc cgccacatta
     9661 acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca taagaagggg tgtttttatg
     9721 tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg
     9781 ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg cgttatttat tccaacaggg
     9841 tggttaccga acgagacgct ggcgaagctg gtcgggccga tgatcactta tctcctaccg
     9901 ctgctgatcg gttataccgg tggtaagctg gtaggcggca aacgtggtgg cgtagtcggt
     9961 gccattacca ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct cggttctatg
    10021 attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg accgctgggt agacggtaag
    10081 atcaaatccg gttttgagat gctggtgaat aacttctccg caggcatcat cgggatgatc
    10141 ctcgctattc tggcattcct cggcattggc ccgattgttg aagccctgtc caaaatgctg
    10201 gctgcgggcg ttaacttcat ggttgtccat gacatgctgc cgctggcgtc tatctttgtt
    10261 gaaccggcga aaatcctgtt cctcaacaac gccattaacc acggtatctt ctcgccgctg
    10321 ggtattcagc agtcccatga actgggtaaa tcaatcttct tcctgattga agctaaccca
    10381 ggtccaggta tgggcgtgct gctggcgtac atgttctttg gtcgtggtag cgctaaacag
    10441 tctgcgggcg gtgcggcaat catccacttc ctgggtggta tccacgaaat ctacttcccg
    10501 tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat gactggcgtg
    10561 ttcacgctga ctatcctggg cggtggtctg gtttctccgg catctccggg ttctatcctt
    10621 gctgtactgg cgatgacacc aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg
    10681 gcgatggctg tctccttcgt tgtctctgct attttgctga aaaccagcaa agtgaaagaa
    10741 gaagatgata ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc
    10801 acatctccgc tatctgcggg cgatgtgact aacgacctga gccacgtacg taaaatcatc
    10861 gttgcctgtg acgccggtat gggttcaagt gcgatgggcg caggcgtgct gcgtaagaaa
    10921 attcaggatg caggtctgtc gcagatttcg gtcactaaca gcgcgatcaa caacctgccg
    10981 ccagatgtgg acctcgtcat cactcaccgt gacctgaccg aacgcgctat gcgccaggtt
    11041 ccgcaggcac agcatatttc gctgaccaac ttcctcgaca gcggcctgta caccagccta
    11101 accgaacgtc tcgttgctgc ccagcgccat actgaaaacg aagtgaaagt gaaagacagt
    11161 ctgaaagaca gctttgacga ttccagtgct aacctgttca aactgggcgc ggagaacatc
    11221 ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg cgagcagctg
    11281 gtgaaaggcg gttacgttga gccggaatac gttcaggcga tgctggatcg tgaaaaactg
    11341 accccgactt atctgggtga gtctatcgcg gtgccacacg gtacggttga agcgaaagat
    11401 cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa
    11461 gaagaagatg acattgcccg tctggtgatt ggtattgcag cccgtaacaa cgagcacatt
    11521 caggttatca ccagcctgac caatgcactg gatgatgagt ccgtcatcga gcgtctggca
    11581 cacaccacca gcgtggatga agtgctggaa ctgctggcag gtcgtaagta atccaatcct
    11641 accctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc cctctcgggt
    11701 aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag gtaatatcgg
    11761 tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt ttgccgatgt
    11821 caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc atgtggtcgg
    11881 tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga
    11941 tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc cggttgtgct
    12001 ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga
    12061 atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggcaccacgc agctgaaagg
    12121 tcatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagagc acgttggctt
    12181 tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta acgatccgct
    12241 ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc
    12301 actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg tcgaacgtaa
    12361 actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac tggccggtca
    12421 tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat
    12481 ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca aacatgcggc
    12541 gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg atgtagagcg
    12601 cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca agccactgct
    12661 cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg ctggtgcaat
    12721 gcacttccgc agtgaagatg atccgcaggc tcaggaactg gcagcactga tcgctgacaa
    12781 aggtccgcag gcggcgctgg cacagatttc cggtcttgat gccaacagcg aggttgtatc
    12841 cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca ggacaccctg
    12901 cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca ggccgttgaa
    12961 aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc
    13021 gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg caaagacgat
    13081 tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct gggcgatctt
    13141 tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga atacgaagat
    13201 gcggaactgc tgatggcatt gcgtgaagag ctaaatcacg acggcaacga gtacgccttt
    13261 accgacgacg aaatccttgg accctttggt gaactgcact gcgtggcggc gttaccaccg
    13321 ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca gcgctatcaa
    13381 caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccaa aatcagctta
    13441 aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg cttcggccga
    13501 taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc atcgggtatt
    13561 ccggacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa cgcctggctt
    13621 gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc taatttgccc
    13681 atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg
    13741 ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca tccgctgctg
    13801 tttattatct ttttctatag atttcttaat taatcaacga attgcgtagt ttttgagcat
    13861 gttaatgatt acgttgcaga actattacaa tgtcatcagt tgtgaacaaa gcacctggtc
    13921 gcgcatacta ggggctataa atttatcttt atcagaagcc atcacatgaa agaagtcgaa
    13981 aaaaacgaaa tcaagcgtct cagcgatcgc ctggacgcca tccgccacca gcaggccgat
    14041 ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg aaaaagagaa agccacgctg
    14101 gaagcagaaa ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa agaagcacag
    14161 aagctgatga agatgccgtt ccagcgcgca attaccaaaa aagagcaggc tgatatgggc
    14221 aagctgaaga aaagtgttcg cggactggtg gttgtgcacc caatgaccgc gctggggcgc
    14281 gaaatgggcc tggaagagat gactggattt tctaagacta cgttttaagc ccctcagacg
    14341 actggttcga tcgtatcaac ccgcatctgg cttgccttag ctggtcaatg cggggactct
    14401 cctgtctact cgcctaatta cc
//
LOCUS       DAJTUM010000093        14334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTUM010000093 DAJTUM010000000
VERSION     DAJTUM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..3051
                     /locus_tag="OO471_003148"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OO471_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OO471_003149"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OO471_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OO471_003150"
     CDS             6768..7175
                     /locus_tag="OO471_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244317.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OO471_003151"
     CDS             7717..8805
                     /locus_tag="OO471_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6244318.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OO471_003152"
     CDS             8816..11095
                     /locus_tag="OO471_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244319.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12225
                     /locus_tag="OO471_003153"
                     /pseudo
     CDS             11088..12225
                     /locus_tag="OO471_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12222..14252
                     /locus_tag="OO471_003154"
     CDS             12222..14252
                     /locus_tag="OO471_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244320.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3766 c         3659 g         3363 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggccc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg accacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt gatgccttcc
    13081 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
    13141 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
    13201 tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc
    13261 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
    13321 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
    13381 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
    13441 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
    13501 ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga
    13561 gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
    13621 ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt
    13681 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
    13741 aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca
    13801 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct
    13861 ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc
    13921 gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg
    13981 ttctgcctgt gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    14041 aactgctcca taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc
    14101 tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc
    14161 gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    14221 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    14281 ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc ctgg
//
LOCUS       DAJTUM010000094        13969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTUM010000094 DAJTUM010000000
VERSION     DAJTUM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            194..2041
                     /gene="ldtD"
                     /locus_tag="OO471_003155"
     CDS             194..2041
                     /gene="ldtD"
                     /locus_tag="OO471_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6244321.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2222..2770
                     /locus_tag="OO471_003156"
     CDS             2222..2770
                     /locus_tag="OO471_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6244322.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2797..3444
                     /gene="gloC"
                     /locus_tag="OO471_003157"
     CDS             2797..3444
                     /gene="gloC"
                     /locus_tag="OO471_003157"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6244323.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3667..4857)
                     /gene="aspC"
                     /locus_tag="OO471_003158"
     CDS             complement(3667..4857)
                     /gene="aspC"
                     /locus_tag="OO471_003158"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6244324.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5042..6130)
                     /gene="ompF"
                     /locus_tag="OO471_003159"
     CDS             complement(5042..6130)
                     /gene="ompF"
                     /locus_tag="OO471_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6244325.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6731..8131)
                     /gene="asnS"
                     /locus_tag="OO471_003160"
     CDS             complement(6731..8131)
                     /gene="asnS"
                     /locus_tag="OO471_003160"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6244326.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO471_003161"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO471_003161"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6244327.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12383
                     /gene="pepN"
                     /locus_tag="OO471_003162"
     CDS             9768..12383
                     /gene="pepN"
                     /locus_tag="OO471_003162"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6244328.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12590..13357)
                     /gene="ssuB"
                     /locus_tag="OO471_003163"
     CDS             complement(12590..13357)
                     /gene="ssuB"
                     /locus_tag="OO471_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6244329.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13354..>13969)
                     /gene="ssuC"
                     /locus_tag="OO471_003164"
     CDS             complement(13354..>13969)
                     /gene="ssuC"
                     /locus_tag="OO471_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6244330.1"
                     /translation="QHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQM
                     LRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSY
                     GLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFL
                     QTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3553 a         3565 c         3527 g         3324 t
ORIGIN      
        1 acataacggc gcgcttttct ttaaacttct ttacattagg ttatgtaaaa acgtatcggc
       61 gtttatatac tgaagataag cctgatgagt aacaggcttg ctcgtcatac tttcgtgagt
      121 attggcgttg tacaggcaag tcgtaaaata acagcctggc tattcagagt atgataaaaa
      181 cagggggcaa gggatgttgc ttaatatgat gtgtggtcgt cggctgtcgg caatcagttt
      241 gtgcctggcc gtaacattcg ctccactgtt caatgcgcag gccgatgagc ctgaagtaat
      301 ccctggcgac agcccggtgg ctgtcagtga acagggcgag gcactgccgc aggcgcaagc
      361 cacggcaata atggcgggga tccagccatt gcctgaaggt gcggcagaaa aagcccgcac
      421 gcaaatcgaa tctcaattac ccgcaggtta caagccggtt tatcttaacc agcttcaact
      481 gttgtatgcc gcacgcgata tgcaacccat gtgggaaaac cgtgatgctg ttaaagcctt
      541 ccagcaacag ctggcagagg tggcgattgc tggtttccag ccgcagttta ataaatgggt
      601 agagttactg accgatcctg gtgttaacgg gatggcacgt gacgtggtgc tctctgatgc
      661 gatgatgggc tatctccatt tcattgcaaa tattccgatc aaaggcactc gctggctata
      721 tagcagtaaa ccttatgcgc tttcaacgcc gccgctttcg gtgattaacc aatggcagct
      781 agcgctggat aaaggtcaat tgcctacgtt tgttgcagga ctggcaccgc agcatccgca
      841 atatgcggcg atgcatgaat cgttactggt cttactcagt gacaccaaac cgtggcccca
      901 actgaccggc aaagcaacgt tgcgcccagg gcagtggagt aacgacgtac cggcgttgcg
      961 cgaaatattg caacgcacag gcatgttgga cggggggccg aaaattactc tacctggcga
     1021 tgacacgcca actgacgcgg tagtcagccc atccgctgtt actgttgaaa cagcagaaac
     1081 taagccgatg gacaagcaaa cgacgtctcg tagtaaacct gcgcctgccg ttcgtgccgc
     1141 ctacgataat gaactggtgg aagccgttaa acgttttcag gcatggcaag gattgggagc
     1201 agatggtgct attggcccgg caacgcgtga ctggttaaac gtaacgcccg cccagcgtgc
     1261 tggcgtgttg gcactcaaca tccagcgatt gcgcttgctg ccaacagagc tttctaccgg
     1321 gatcatggtt aacattccag cctattcgct gctctactat cagaacggca atcaggtgct
     1381 ggattcgcga gtcattgtcg gtcgccccga tcgcaaaacg ccgatgatga gcagtgcact
     1441 taacaacgta gtggtaaacc cgccgtggaa cgtaccacca actctggcac gcaaagatat
     1501 tctgccgaaa gtgcgcaacg atccgggata tctcgaaagc catggctata cggtgatgcg
     1561 cggctggaac agcagagaag cgattgaccc atgggaggtt gactggtcta caatcacggc
     1621 ctcgaattta ccgtttcgct tccagcaggc tccaggccca cggaactcgc tggggcgcta
     1681 taaattcaat atgccgagtt cagaggccat ttatttgcat gacacgccga accacaatct
     1741 gttcaagcgt gatacacgcg cattgagctc aggctgtgta cgagtgaata aagcttccga
     1801 tctggcgaat atgctgttgc aggatgcagg atggaatgac aaacgtattt ctgatgcgct
     1861 gaagcagggt gatacacgtt acgtcaatat tcggcagtcg attccggtga atctctacta
     1921 cctgacggcc tttgttggtg cagatgatcg tacccagtat cgtacagata tttacaatta
     1981 tgatctgcct gcgcgatcca gctcgcaaat cgtatcgaaa gcggaacaat taatcaggta
     2041 aatgaagtag ttcggggaaa tcaattgtcg taataattag catgattggg ggcgattctc
     2101 tgcagccccc gtcactgctg gggttgagtc atcttgacgt ctgctttacg ggcggttaag
     2161 gtgcctcttg tgcgccagaa gtgcatataa acgataacat tgagctgtag acttgattat
     2221 catggacaaa ttcgacgcta atcgccgcaa attgctggcg cttggtggcg ttgcactcgg
     2281 tgccgccatc ctgccgaccc ctgcgtttgc aacactctct accccacgcc cgcgcatttt
     2341 gacactcaat aatcttcata ccggagagtc aatcaaagcg gagtttttcg atggcagagg
     2401 ctatattcag gaagaattgg caaaacttaa ccattttttc cgcgattacc gcgcgaacaa
     2461 aataaagtcc atcgacccag gattattcga ccagttgtat cgcctgcaag ggttgttagg
     2521 cacgcgcaaa ccggtgcaac tcatttccgg ttatcgttcc attgatacca acaatgaact
     2581 acgcgcccgc agccgtggag tagcgaagaa aagctatcac actaaaggcc aggcgatgga
     2641 tttccatatt gaaggtatcg cgttaagcaa tattcgcaaa gccgcgttat ctatgcgcgc
     2701 aggtggtgta ggatattacc cacgtagtaa ctttgtgcat attgataccg ggccagcacg
     2761 gcactggtag taatcgctta acgaaacagg ggcagtatga actatcgtat tattccggtc
     2821 accgcattct cccagaactg ttcattgatc tggtgtgaac aaacccgcct ggccgcactg
     2881 gtcgatcctg gcggcgatgc ggaaaaaatc aaacaggaag ttgatgacag cggcctgaca
     2941 ctgatgcaga tcctgctgac gcatggtcat ctggaccacg ttggcgcagc ggcggaactg
     3001 gcgcaacatt acggcgtgcc ggtttttggc ccggaaaaag aagatgagtt ctggctgcaa
     3061 ggcttgcctg cgcaaagtcg tatgtttggt ctggaagagt gccagccgct gacgccagat
     3121 cgttggctga acgaaggcga taccatcagc atagggaatg tgactttaca ggtgttacat
     3181 tgccctgggc atacgccggg tcatgtcgtg ttttttgatg atcgggcaaa gctgctgatt
     3241 tctggcgatg ttattttcaa aggcggagta gggcgcagtg acttcccgcg tggcgatcat
     3301 aatcaactga tttcttcaat caaagataaa ttgctgccac tgggggatga cgtgacattt
     3361 attccgggtc acggaccatt atctacactt ggttatgagc gcctgcacaa tcccttcctg
     3421 caagacgaaa tgcccgtctg gtaaaggcgc ataaaaaagt ccacttttaa tgcgggcctg
     3481 gatttctggc aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct tatccggcct
     3541 acaaaatcgt gcaaattcaa aatattgcag gggacgcgta ggcctgataa gcgtagcgca
     3601 tcaggcaatg ttgcgtttgt catcagtctc agcccgcttt tcagcgggct tcattgtttt
     3661 taacgcttac agcactgcca caatcgattc gcacagcgga gccatgttat ctggtgtcat
     3721 cccggccaca tttacgcgac cagaagcaac agcatatacg ccaaactctt cgcgcagacg
     3781 cagcacttgt tcttttgtca gaccactgaa ggagaacatg ccgttctgtt tgatgataaa
     3841 gctgaagtcg cggtttgcac ctttttcctg cagcgtattg acgaacaact gacgcatacg
     3901 ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta atgcatcgct
     3961 gctcaggatg gtggcaacaa cagaagcgcc gtgtgctgga gggttagagt agttagcgcg
     4021 aatcgccgct ttcatttggc tgaatgcgcg atcaacgatt tcactgtcgg cagcaaccag
     4081 agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac
     4141 aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc
     4201 acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caacggagag
     4261 ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt tatggcagca
     4321 gccatggaac agcactacgt cgccagcctg agcttcgttc agactgttaa tcagtgcatc
     4381 gaagtcaagg gtgtgatttt ccgcatcata ataagcgtat tcacgaactt ccagacctgc
     4441 agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca cacgcttaac
     4501 gctggtattt tttgccagga agtcggcagc cacgcgtagt gcgccagtgc cacccggagt
     4561 ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc
     4621 ctgagtgcag cgaccaaatt caaggatgcc gtcaatgccg aggtaatttt tggtggtttc
     4681 attttcgagc agatactgtt cagccttttt cacgctggtc agtaccgggg ttttgcccgt
     4741 ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa
     4801 cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac
     4861 gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa
     4921 attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga
     4981 aggcataaaa aaaacaggac caacgtcctg ttttttcggc atttaacaaa gaggtgtgct
     5041 attagaactg gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg cccagtttgt
     5101 tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt
     5161 agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt
     5221 ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt
     5281 gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttat
     5341 tagtgatcgg cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg
     5401 cgtcgtactt cagaccagta gcccactgtt cagctttttt accgttgcca agaggttgag
     5461 cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt
     5521 attcgtagct gatagaaccg ccaacaccgt cgccgttaga acggcgtgca gtgtcacgct
     5581 cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg
     5641 agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag
     5701 tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt
     5761 agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac
     5821 gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt
     5881 cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac
     5941 gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggaaaaat
     6001 aatgcagacc aacagtttta ccgtacagat ctactttgtt gccatctttg ttatagattt
     6061 ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc
     6121 gcttcatcat tatttattac cctcatggtt tttttatgac acctgccact gccgtcaata
     6181 agttctgtca ataaaaattt acggaactat tgatgagagt ttggtgtctt tatgtgtctg
     6241 caggcatctt tccattcaaa ctaacgtttc gctaccgtga aagtgctaca aagataaaga
     6301 tttagtttca aaaagaaaaa atatgtaacc aaaagtaaaa attaaggagc tttgtgaaca
     6361 ccgtcatatt tccatagaaa cgtgatgata tttacagcaa tttttatcta tttatatgat
     6421 ttacttatat ttaaattaac taaacggaaa ttttgtttct gatggaaact ttatcgacct
     6481 ggcacaaaat cctctttcag acatccagaa tgccaaaaaa tagtatgaaa attgtgctat
     6541 cagctaagaa aaaactaatc cgcaataaga gtacgggtta ttttttgtgc gtaaatgttg
     6601 caatgtaata ttccgtaaca ggatgatcgt tatggcgggg gtgcaaatag attgcttgac
     6661 ttaacaaaca gataacttga cagaaaggat aaaaaagagc cagcaggcgc tggctctttg
     6721 aggaagaaaa tcagaagctg gcgttgcgcg gagtacgtgg gaacggaatc acatcacgca
     6781 cgttttgcac gccagttacg taagcaatca ggcgttcaaa accaagaccg aaacctgaat
     6841 gcggaacagt accgtagcga cgcagatcgc gataccacca gtaatcttct ttattcaggc
     6901 ccatttccag catacgctcg tccagaacgt ccagacgttc ttcacgctgg gagccaccaa
     6961 tgatctcacc gatgcccgga gccagaacgt ccatagccgc aacggtttta ccgtcttcgt
     7021 tgaggcgcat atagaacgct ttaatatctt tcggatagtt tttaaccact accggtgctt
     7081 taaagtgttc ttccgccaga taacgctcat gctcagaaga gagatcgacg ccccagtaaa
     7141 ccgggttttc aaacttcctg ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca
     7201 cctgcgcgaa atcggcttca atgaagcgtt ccagacgtga aacggcatct ttatctacgc
     7261 gttcagcgaa gaatttcatg tcgtctgcgc gttcttcgag aaccgctttg aagacatatt
     7321 tcagcatggc ttcagccaga cccgcgatat cgttcaggtt agcaaacgcc acttccggct
     7381 ccagcatcca gaattccgcc aggtgacggc tggtgttgga gttttcagca cggaaagtcg
     7441 ggccgaaggt ataaattttg gataatgcgc aagcgtaggt ttcgccgttc aactggccag
     7501 atacggtcag gaaagactct ttaccaaaga agtctttgtc gaaatccact ttgccctgat
     7561 cgttacgcgg caggttttcc agatccagcg tagaaacgcg gaacatttcg cctgcacctt
     7621 cggtatcaga tgcggtaatc agtggcgttg aaacccagaa gaatccctgc tcgttaaaga
     7681 agcgatgcag cgcctgcgcc agcgtatggc gaacgcgcgc gacggcacca atcaggtttg
     7741 tgcgcggacg caggtgagcg acttcacgca gatactcaat gctgtggcgt tttgccgcca
     7801 tcgggtaagt gtctggatct tcaacccaac cagcaacttc aaccttgctg gcctgaattt
     7861 caaattgttg cccctggccc ggcgatgcca cgactttacc cgtcacaatg accgagcagc
     7921 cggtggtcag acgcaggacg tcttcattgt aattgggcag agaattattg atgacagcct
     7981 gtacaggatc aaagcaggaa ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat
     8041 ctcggcgggt acgtacccat ccgcgtacgg tgacttcgct gtcaacggct acgcggccct
     8101 ggagtacgtc ggctacaggc acaacgctca taatattctc tctgttaata gtcggaaaaa
     8161 ataaacactt gtccacccgt aaatgggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccggatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctccg cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga agagggcgca ctggtcatca
    10021 gtaatttgcc ggagcgtttt acgcttaaga tcattaatga aataagcctg gcggcgaata
    10081 cggcgctgga agggctttat cagtcaggcg atgcgctttg cacccagtgt gaagccgaag
    10141 gtttccgcca tattacgtat tatctcgacc gcccggacgt gctggcgcgt tttaccacca
    10201 aaattattgc cgataaaatc aaatatccct tcctgctttc caatggtaac cgcgttgcgc
    10261 aaggcgaact cgaaaacgga cgccattggg tacagtggca ggacccgttc ccgaaaccgt
    10321 gctacctgtt tgcgctggtg gcaggcgact ttgatgtact gcgcgatacc tttaccacgc
    10381 gttctggtcg cgaagtagca ctggagctgt acgtcgatcg cggcaacctt gatcgcgcgc
    10441 cgtgggcgat gacctcgctg aaaaactcca tgaaatggga tgaagaacgc tttggcctgg
    10501 agtatgacct cgacatctat atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg
    10561 agaataaggg tctgaatatc tttaactcca aatatgtgct ggcccgcacc gacaccgcca
    10621 ccgacaaaga ttacctcgat attgaacgcg ttatcggcca tgaatatttc cataactgga
    10681 ccggtaaccg agtgacctgc cgcgactggt tccagctcag cctgaaagaa ggtttaaccg
    10741 tcttccgcga tcaggaattc agctctgacc ttggttcccg cgcagttaac cgcatcaata
    10801 atgtgcgcac catgcgcgga ttgcagtttg cggaagacgc cagcccgatg gcgcacccaa
    10861 tccgcccgga catggtcatt gagatgaaca acttctacac cctgaccgtt tacgagaagg
    10921 gcgcggaagt gatccgtatg atccacaccc tgctgggcga agaaaacttc cagaaaggga
    10981 tgcaactcta tttcgagcgt catgatggta gcgcagcgac ctgtgacgac tttgtgcagg
    11041 cgatggaaga tgcgtcgaat gttgatctct cccatttccg ccgttggtac agccagtccg
    11101 gtacgccggt tgtgaccgtc aaagacgact acaatccgga aactgagcag tacaccctga
    11161 ccatcagcca gcgcacgcca gccacgccgg atcaggcaga aaaacagccg ctgcatattc
    11221 cgtttgccat cgaattgtat gacaacgaag gtaaagtgat cccgttgcag aaaggcggtc
    11281 atccggtgaa ttccgtgctg aacgtcaccc aggcggaaca gacctttgtt tttgataatg
    11341 tctacttcca gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg aaactggaat
    11401 ataagtggag cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat ggtttctccc
    11461 gttgggatgc ggcgcaaagt ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc
    11521 agcaagggca gccgctgtct ctgccggtac atgtggctga cgctttccgc gcggtgctgc
    11581 tcgatgagaa gattgatccg gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg
    11641 aaatggctga actgttcgat atcatagacc cgattgctat tgccgaagta cgcgaagcac
    11701 tcactcgtac tctggcgact gaactggcgg atgagctgct ggctatttac aacgcgaatt
    11761 accagagcga gtaccgtgtt gagcatgaag atattgcaaa acgcactctg cgtaatgcct
    11821 gcctgcgctt ccttgctttt ggtgaaacgc atctggctga cgtgctggtg agcaagcaat
    11881 accatgaagc aaacaatatg accgatgcgc tggcagcgct ttctgcggcg gttgctgcac
    11941 agctgccttg ccgtgacgcg ctgatgcagg agtacgacga taagtggcat caggacggtc
    12001 tggtgatgga taaatggttt atcctgcaag ccaccagccc ggcggcgaat gtgctggaga
    12061 cggtgcgtgg tctgttgcag catcgctcat ttaccatgag caacccgaac cgtattcgtt
    12121 cgttgattgg tgcgtttgcg ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg
    12181 gttaccagtt cctggtggaa atgcttaccg acctcaacag ccgtaacccg caggtagctt
    12241 cacgtctgat tgaaccgctg attcgcctga aacgttatga tgccaaacgt caggagaaaa
    12301 tgcgcgcggc gctggaacag ttgaaagggc tggaaaatct ctctggcgat ctttacgaga
    12361 agataactaa agcactggct tgatactttt gagccaaaat gcctgatgcg acgcttgacg
    12421 cgtcttatca ggcctacaac tccgttgaaa ttgctggttt tgtaggctgg ataaggcgtt
    12481 cacgccgcat ccggcagccg tgcccaactg cctgatgcga tgcttgacgt gtcttatcag
    12541 gcctacaagt ccgttgaaaa tgctggtttt gtgcgccaca tccggcatat taaccatgac
    12601 gccgaatcag ttgttcagaa tgcccacgcc gcattacccg ttgcaatact tccgcttcca
    12661 gctccgctaa tcgcaccgac cctaatcgac gcggacgggg gatatccact gtcaaatcca
    12721 gactaatttt tccctcttca attaacaaca cccggtcagc cattgctacc gcttcgctca
    12781 catcatgcgt caccaacagt acggtaaagc cgtgctgctg ccaaagtgac acaatcaaat
    12841 cctgcatctc gagtcgcgtt aaggcgtcca gtgccccgag cggttcatca agcaacaata
    12901 aacccggtcg atgaatcaac gctcttgcca gcgccacacg ctgtttctgc ccgccagaaa
    12961 gtgcggcagg ccattccccg gcgcgattct ccagtcctgc cgcagccaac gcccgacgcg
    13021 cggcatcgcg ccactgacct ttaaggccta acccaacgtt atcaatcacc gatttccacg
    13081 gcagcagacg cgcatcctga aacatcattc gcgtatcatc ctgaatttca gccagcggtg
    13141 tggtgcccgc taacacatcg cctgcggttg gcgtttccag accggccagc aggtgcagca
    13201 gggtactttt accaccaccg ctgcggccca ccaccgccac aaactgacct gccggaatat
    13261 gtaaatccag ttggttcagg acgatatttt ccgcgtaatg tttgcttact gcattgagca
    13321 acaatggtgt gccctggttc agacgagcag tattcatatc gtggcctcct tcaaatgata
    13381 agccgggttc cagcgcaacc agaggcgctc taacagctgc gcgctgacgt cagcaagttt
    13441 gccgagcagg gcgtaaagaa taatagcgac taccaccacg tctgtttgca aaaactctcg
    13501 cgcattcatc gccagataac caatgccgga attggcagaa atggtttcgg caacaatcag
    13561 cgtcagccac atcaggccca acgcaaaacg cacgccgacc ataattgagg gcagggcatc
    13621 gggcaggatc acatggataa acagcggtat gccggataat ccatagctac gcgccatctc
    13681 caccagcccg cgatcgatat tacggatgcc atgccaggtg ttgatataaa tggggaacag
    13741 cgtaccgagc gccaccagaa agattttcgc ggattcatca atgccaaacc acaaaatcac
    13801 cagcgggatc agcgccagat gcggcacgtt gcgcaacatc tgaattgagg tatccagcag
    13861 cctttctccc cagcgcgaca gtccgctaat cagtcccaga atcaggccca gcgatccgcc
    13921 aattgaaaag ccaatcagcg cccgccagga actgatcgcc agatgctgc
//
LOCUS       DAJTUM010000095        13966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTUM010000095 DAJTUM010000000
VERSION     DAJTUM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            665..1456
                     /locus_tag="OO471_003165"
     CDS             665..1456
                     /locus_tag="OO471_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244331.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1842..2048
                     /locus_tag="OO471_003166"
     CDS             1842..2048
                     /locus_tag="OO471_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6244332.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2134..2706
                     /locus_tag="OO471_003167"
     CDS             2134..2706
                     /locus_tag="OO471_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244333.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2708..2992)
                     /locus_tag="OO471_003168"
     CDS             complement(2708..2992)
                     /locus_tag="OO471_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244334.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2963..3106
                     /locus_tag="OO471_003169"
                     /pseudo
     CDS             2963..3106
                     /locus_tag="OO471_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4275..5294
                     /locus_tag="OO471_003170"
     CDS             4275..5294
                     /locus_tag="OO471_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6244335.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5404..6276
                     /locus_tag="OO471_003171"
     CDS             5404..6276
                     /locus_tag="OO471_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6244336.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6610..9732
                     /gene="ag43"
                     /locus_tag="OO471_003172"
     CDS             6610..9732
                     /gene="ag43"
                     /locus_tag="OO471_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6244337.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9853..12369
                     /locus_tag="OO471_003173"
     CDS             9853..12369
                     /locus_tag="OO471_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244338.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12445..12900
                     /locus_tag="OO471_003174"
     CDS             12445..12900
                     /locus_tag="OO471_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244339.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(13019..13230)
                     /locus_tag="OO471_003175"
                     /pseudo
     CDS             complement(13019..13230)
                     /locus_tag="OO471_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13311..>13966
                     /locus_tag="OO471_003176"
     CDS             13311..>13966
                     /locus_tag="OO471_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6244340.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     T"
BASE COUNT         3793 a         3441 c         3431 g         3301 t
ORIGIN      
        1 ctcttgacta taaagatgat gacgggatca gaacagttat taatccatac aacaaaatac
       61 agaaaacaac agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat aacagacatt
      121 acttaacaaa gaacattaca gtcagtgtgt acattacaga tttatattca ggtgttgcgg
      181 gactacatta cagcagagag gggtaaggac agagttacaa gtactactag agagaatatc
      241 actgcacaga tacttctatg agtatatctt tatgagagat ccgtctacag gattgggtta
      301 taaggtgtat ttacaaggta taatcacagg atcatttaca tgaccatact tctgtaaata
      361 atactaatta accttaatta tttttttatg gtacatgaac tacctgtagc tgtaacgata
      421 aacaaatcac ccaaaatgaa aaatactggt tatcacagtt cagagaactc gtatgagtgt
      481 ccccctgagc aatgcttcat tttctgtgat tctgtgtgat agagttatac tgagtacagt
      541 ggcagtaaca ctgctttcca tcacttgagg ataaaattca ttaaaaaatt aattgcatga
      601 attaacaggt ttttttatga aatgttatgc atctcaacca atatgattaa cagaaaactt
      661 catcatgaca acacgtataa cgttcagtat gaccagtgat gagacgctgc gaattgtgga
      721 tgagtactgt catacccaca aactttcgcg ctctaaggta atcaacgcat tactcagcgc
      781 cacagcacct gtccttaatg atattaactg ttattatcaa cttgcaggcg aactgcagtc
      841 acgcttatta aatggagtgt atcaacggga tcttcctcgc aaacgcaatg tcgtcagtgc
      901 agagaagtat tgtctggaaa tctgggaaaa caaacttttc accagaagaa tcctggagtt
      961 tgacagttca aatggtgttc tgtacgcgct gaaacataaa agacactatc gccgggataa
     1021 gatgattgga agggttgaaa gtcggtgtat caaagacatt tgcgaatacc agatgcagct
     1081 atccggggag aagacgaaat atgcctgttt tatttatatc gaaagaacaa tatacaatca
     1141 cgataatcca tctgataaaa tacctgtaaa agcggctgtt ggtaatgcag tcatcctgct
     1201 ggcaaaggat gtgatatata acgagtactt ttttgatttg cggcagtcat tttttgtgag
     1261 cgttacagat ctgatggtct ctggcgcaaa aggtattccg gagacacaga catatccgga
     1321 cgtttattgc tggatccctc ttttttccat aaattccggc gtgctcatta cccctgtata
     1381 taaaattgac cctctgaaac ccgtaacggt aaaaaaaccg gataagatca ctgttgtttg
     1441 taattacaga gaataatttc agccttctac cttcaccgcg acagtgttta cctctgcttt
     1501 atcttccgtt cagtaaatac aaaccaccgc tctggcaggt acagggcgtg gtttatctcc
     1561 gatactcccc tgtgcagtaa taatcatcac tgaaaatact gatcccgtca cggcaaaacg
     1621 ccatcccata ttccggcaac acaaaatcat cttccgttta taccggattc actgacaaaa
     1681 cagacaacct gtattgcctg tacacaacag tttctctcct ggcaggaata ttcccctact
     1741 cattttttac gtttctcccc ggttgttatg ccggtgaagg tggtgcgtcg ttttcatcac
     1801 caacgccggt tgagattaac aacatccgga ggaacattct catgaatacc ccagtttcac
     1861 tgatggatga ccagatggtc gacatgtcgt ttatcactca gctgaccggc ctgaccgata
     1921 agtggtttta taaactcatc aagcatggag cctttccgcc ccccatcaaa ctgggccgta
     1981 gctcccgctg gctgaaaagc gaagtggaag cctggctgca ggcgcgtatt gcacagtccc
     2041 gtccgtaatt cctgaccgtt atccgttcca ccgcagcaaa ggcatgatcc ggcccccgga
     2101 ggcattttgc tgcgcgctaa aggagagaat attatgcaac aacttgaaaa caggtgtgac
     2161 ctgctgctca tccagcatca gaaatggatg acaagcgtga cgaggcttat cgtcgctcat
     2221 ggaatggggt ctccgcatct tcatggttat caccgtctga ccctggcaca cttttttctg
     2281 ccggaaaaag gctccgtcat ttcggtggcc ccccagggac tctatcaggt cgtcaacccg
     2341 ggaacaccac cgttcattcc tgccattcag gaagggttaa tgaccagcat tcagacccat
     2401 gaaatcatgc tgcttacaca ttttaatctg ggcggcgttt tactcagtga gctacatcgt
     2461 ctgggtgaaa accggctggc aaacaggctg aacagcctgc tccggcgttt cgacgatcgt
     2521 gatctctacc acacgctgat atggctgtgc tggtatgatc tgatgtgtgc ccactccatg
     2581 cagccctgga ctgaagagtt aaagcataaa tcacatgcag agcttgaaaa ctgggctgtg
     2641 gcgcgcaaac gggaaaagcg cgagctggaa ctgatgattg acgagtacct gttatacgcc
     2701 tgctgattca taacgttccc ggggttcctt cctcatcgtt acacacagcc ggacggctat
     2761 ctatggctgt ccgccaaatg accatcgccg catttcgttc cgctgaaaac ggtacgtagt
     2821 cagtgatacc ggaatttccg gagagggccg caggtatatt gcttctgtgc aattgatgct
     2881 tatttacatt ggatgtagtt atttctgcat ccaatgctat cggaaagaga ttaaccagca
     2941 aatggttcct caaatcgtat tgatatttac ccggcgtatt gagcaccacc atctgacact
     3001 ccgttcccgc atcaaacgac tggaccgaaa aacgatttac ttttcacgtg ctgtggaaat
     3061 tcacgaaaaa attattgggt cattcatcga aaaacatatg ttctgatgac tggagtcatc
     3121 ctccagaaat atacgtcaac tgaacaatat cgatttttta tgggattcta tacacttttc
     3181 catatccctc tgaaaatcag tcatcagctc accaacacct cttctgaaac ttgtattaaa
     3241 cttattgctt aaatagatga tataacatct cttccattgc actgaatcgg cgaaaactgc
     3301 cgtttcaata atctcaacaa tattatttat cggctaactt tatcagtaaa caatggaatg
     3361 tcacactgat gaaatgaaca ttcagagtaa gatgataaaa ttcatataaa cgccattgct
     3421 gaatattatt tatttcatac agataaacga taaaatacaa aagggtatat ttctcaatgt
     3481 aacagtcaat tattcatctc cactggcgag cacggtcagc acacaaacaa agttagtcct
     3541 cctgcttaat cattgcgtat cagaaactaa aaaagaaaaa attctgcaga caacttaaca
     3601 atcttcatca catttcctga agacagacat atccacaaac ggtagtcagc gttgaattaa
     3661 caagtcttac agagctatac gtagcttata aagccactca atgttcatca tcacccgact
     3721 acttatctgg aataccacca gtataaactc atcaataaaa acacctgttg ttaaaataag
     3781 aatactgtca gcagtaaatt attaaaacaa catctgtcca cactatctgt tcttttaacg
     3841 ccaacggcag cgaaaatagt gcatcacagc cttctttttt ctttcgccac cactcagaca
     3901 aacacaacac tcacacccac accacaaaac agaatcaaaa cataaaatta acaataacag
     3961 tcaaaaggaa aacacagaat attgaggtgg actgtcacca ggacagcgca tacgacgcat
     4021 gtgatcagca catcccgttc ttcgctggca tccccaaaat gcagaacaaa caatcataac
     4081 attatgaata taaaagaaaa tatgattttt caccatgaaa aaaacatcaa tgtatctctg
     4141 tataaaaata atgataacat gacataaaca tttaaaaaca taaataaaca gcaacaaaaa
     4201 catttgataa tcacttagtg attaaataca attaagacga ttttgttata agagaggcac
     4261 catggagtga gataatgaat aacgaacaga tgcttatcaa atgcagagat ccccgtaaat
     4321 gtcggtttta tgccgaatat ctgaatcaca aataccctaa tgaattccct cactttgcgc
     4381 ctgacaaccc acaaaattgt atcaggaatg ttaatttatt tttaaaggaa aagtattgta
     4441 atgtttcacc tccccccttt ggtacttttg gtactactga agaagaagca ctcaataacg
     4501 aaattaatga gcttgaaatt attgccgggc aggcaatact gcctgaagag agttttctct
     4561 ggttgaaaac tgacgaactt gcgactttct ttacctggac tactatttat ctgtcgaagg
     4621 aagaccatac aaaattcccg aaaataaacc ataattcatc aggaataaaa aacggaattg
     4681 aagtcaggaa aatacgaaca gttaccgatg aaacgttaaa cacttacaaa aaaagtaacc
     4741 ttccggataa tccatcatcc cataacgaac gaataaaagt aatcatttca tattttgact
     4801 cctgccattc tttaattaat aaaaatgcaa aaagcatata cctcgaggaa ctcaggcagg
     4861 cgtggcttaa aaagaaaagc gcttgtcaaa agtttaagtg gctccttcct gacgatgatg
     4921 aaatgtgtca gtggttctgg acaagactga aacaaaaaca ggatgaaaaa catccagtac
     4981 ataacccagt gacccggtgg ttcataccat cttcaacgaa agaatattat cttgcctctc
     5041 ttgtcgcctt tgctttatgg aaagaagcgc ctgataccat tgaactgttc agaacacgaa
     5101 taaatcatgc ctggcatcag aaaaaacaaa aggacaaata tcggagtgat ggtaaaaagg
     5161 ccattaatat acatataaga agcaatgtga aagaaatgct tgatgagctc agaactgttt
     5221 acggtctgag aacaggagag ttttttgaaa tgctgattac agagaaatat cttgaacaca
     5281 agcgaaggaa gtaattcatt acctgttaca ttctaaataa tctcagacag atattatctc
     5341 cgtgaatccc ccgccacctt tccggtgcgc ggggttttgt cttttttcac cgggaataca
     5401 tgtatgaatc cgtctgatgc cattgaggca attgaaaaac cgctctcctc cctgccttac
     5461 tcactttccc gtcacatcct ggaacatctg cgcaaactca ccagtcacga acccgtgatt
     5521 ggcattatgg gtaaaagcgg ggccggtaaa tcttcactct gtaatgcact gtttcagggg
     5581 gaggtcacac cggtcagtga tgttcacgcc ggcacccggg aagtgcagcg cttccgtctg
     5641 agtggtcacg gtcacagcat ggttatcact gacctgcccg gggtgggcga gagccgggac
     5701 agggatgcag agtatgaagc cctgtaccgt gacattctgc ctgaactgga cctggtgctg
     5761 tggctgatta aagccgatga ccgggccctg tctgtggatg agtatttctg gcgacacatc
     5821 ctgcagtgcg gacatcagca ggtgctgttt gtggtgacgc aggctgacaa aacggaaccc
     5881 tgccatgaat gggatatggc aggtattcag ccttctcccg cacaggaaca gaacattcgt
     5941 gagaaaacgg aggcggtatt ccgtctgttc cggcccgtac atccggttgt tgccgtatcg
     6001 gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc tcatgacagc gcttcccgac
     6061 catgccgcca gccccctgat gacccgactg caggacgagc tgcgcacgga gtctgtccgc
     6121 tctcaggccc gtgaacagtt taccggtgcg gtggaccgga tatttgacac agcggagagc
     6181 gtctgtgttg cctctgttgt ccgtacggcc ctgcgcgctg ttcgtgacac cgtggtctct
     6241 gttgcccgcg cggtatggaa ctggatcttc ttctgaacct gttgtggatg atgtcctccc
     6301 tgcctctgag tctgctcaca aaagcgctgt tttcgttact gtctctcttg tccgtgcaat
     6361 agactgataa tagaataaaa cgatcgatac ctattttacc gatcgttaat atcgatcgtt
     6421 atgctaataa taactcctgt tagcaacgtg cgcagataca cacagacatg agattcaggg
     6481 aacaacagag ccacacgtca gaaacttccg tcagccggac ctccggcact gtaacccttt
     6541 acctgccggt atccacatct gtggataccg gcttttttat tcaccctcac tctgattaag
     6601 gaaatgctga tgaaacgaca tctgaatacc tgctacaggc tggtatggaa tcacattacg
     6661 ggcgctttcg tggttgcctc cgaactggcc cgcgcacagg gtaaacgtgg cggtgtggcg
     6721 gttgcactgt ctcttgccgc ggtcacgtca ctcccggtgc tggctgctga catcgttgtg
     6781 cacccgggtg aaacagtgaa tggcggaaca ctggtaaacc atgacaacca gtttgtatcc
     6841 ggaacagctg atggcgtgac tgtcagtacc gggcttgagc tggggccgga cagtgacgaa
     6901 aacaccggcg ggcaatggat aaaagcgggt ggcacaggca gaaacaccac tgtcaccgca
     6961 aatggtcgtc agattgtgca ggcaggagga actgccagtg atacggttat tcgtgatggc
     7021 ggagggcaga gccttaacgg actggcggtg aacaccacgc tggataacag aggtgagcag
     7081 tgggtacacg ggggagggaa agctgccggt acaattatta accaggatgg ttaccagacc
     7141 ataaaacatg gcggactggc aaccggaacc atcgtcaaca ccggtgcaga aggtggtccg
     7201 gagtctgaaa atgtgtccag cggtcagatg gtcggaggga cggctgaatc caccaccatc
     7261 aataaaaatg gccggcaggt tatctggtct tcggggatgg cacgggacac cctcatttac
     7321 gccggtggtg accagacggt acacggagag gcacataaca cccgactgga ggggggtaac
     7381 cagtatgtac acaacggtgg cacggcaaca gagacgctga taaaccgtga tggctggcag
     7441 gtgattaagg aaggaggaac tgccgcgcat accaccatca accagaaagg aaagctgcag
     7501 gtgaatgccg gcggtaaagc gtctgatgtc acccagaaca cgggcggtgc actggttacc
     7561 agtacagctg caaccgtcac cggcacaaac cgcctgggag cattctctgt tgtggagggt
     7621 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     7681 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     7741 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     7801 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     7861 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     7921 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     7981 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     8041 ggcgatgcac tcctgcaggg aggcgctctc accggtaacg gcagcgtgga aaaatcagga
     8101 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     8161 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     8221 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     8281 ctcgcctctg gtgccacctg gaatattccc gataacgcca cggtgcagtc ggtggtggat
     8341 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     8401 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     8461 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggtggcag ggcaaccgga
     8521 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     8581 aagggtattc aggtggtgga agccattaac ggtgccacca cggaggaagg ggcctttatc
     8641 caggggaata agctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     8701 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     8761 ctgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt
     8821 gtaagcggtg aaaataacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     8881 aacaacggcg gtattgcccg tggagccacg ccggaaagca gcggcagcta tggcttcgtc
     8941 cgtctggagg gtgacctgct cagaacagag gttgccggta tgtctgtgac cgcgggggta
     9001 tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc ccgtgccggc
     9061 acggtccggg atgatgccgg cagcctgggc ggatacctga atctgataca caacgcctcc
     9121 ggcctgtggg ctgacattgt ggcccaggga acccgccaca gcatgaaagc ctcatcggac
     9181 aataacgact tccgcgtccg gggctggggc tggctgggtt cgctggaaac cggtctgccc
     9241 ttcagtatca ctgacaatct gatgctggag ccgcaactgc agtacacctg gcagggactc
     9301 tccctggatg acggccagga taacgccagt tatgtgaagt tcgggcatgg cagtgcacaa
     9361 catgtgcgtg ccggcttccg tctgggcagc caccacgata tgaactttgg taaaggcacc
     9421 tcatcccgtg acaccctgcg cggcagtgcc aaacacagtg tgcgtgaact gccggtgaac
     9481 tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggggacat gagcatgggt
     9541 acagccgcag ccggcagtaa catgacgttc tcaccgtcac agaatggtac gtcactggac
     9601 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccagt
     9661 tatgcccaca gcatcaacgg cagcagcgct gaaggttata acagtcaggc cacactgaat
     9721 gtaaccttct gataattctg cattgcctct ctgtggtccc ggtcatgatg accgggaccc
     9781 ggacggggga aaacgtttca gtgccacatt cactggcatt cacaataaca tgatattcat
     9841 cacggagtga ctatgttaca gatagtcggc gcgctgattc tgctgatcgc aggatttgcc
     9901 attcttcgcc ttttgttcag agcattaacc agcacagcgt ctgcgctggc agggttcata
     9961 ttgctgtgtc tgttcggtcc ggctttactg gctggctata tcactgaacg cataacccgg
    10021 ttattccata ttcgctggct ggcaggcgta tttctgacga ttgccggaat gatcatcagc
    10081 ttcatgtggg gacttgatgg taaacatatc gcactggagg ctcatacttt tgactctgta
    10141 aaatttattc tgaccaccgc tctcgccgct ggtctgctgg ctcttcccgt gcagataaga
    10201 accattcagc agaacgggct cacacctgaa gatatcagca aggaaattaa cgggtattac
    10261 tgctgttttt atactgcttt tttccttatg gcgtgttctg catacgcacc attgatcgca
    10321 ttgcagttcg atatttcacc ctcactgatg tggtggggcg ggttgttgta ctggctggct
    10381 gcattagtga cgctgctatg ggcggccagc cagatccagg cgctgaaaag actgaccagt
    10441 gccatcagcc agacactgga agaacaaccg gtgctcaaca gtaaatcgtg gctgagcagt
    10501 ttgcaaaacg attacagcct tcctgaaacg ctgacggagc gcatctggct gacactcatt
    10561 tcacaacgga tttcccgggg agaactgagg gaatttgaac tggcagacgg aaactggtta
    10621 ctgaacaatg cctggtatga aagaaacatg gcagggttta acgaacagtt gaaagagaac
    10681 ctgtcattca cacctgatga actgaaaacg ctcttccgga accgcctgaa tttatcaccg
    10741 gaagcgaatg acgattttct cgatcgttgc ctggacggcg gtgactggta ccccttttca
    10801 gaaggccgcc gttttgtatc attccaccac gtggatgagc ttcgtatctg tgcctcctgc
    10861 gggctgacag aagtacatca tgccccggaa aatcataagc cggctccgga atggtactgc
    10921 tcctctcttt gtcacgaaac agaaacactg tgtcaggaca tttatgaacg ttcttacacc
    10981 ggttttattt ccgatgcaac ggcgaatggt ctgattctca tgaaactgcc ggaaacctgg
    11041 agtacaaatg agaaaatgtt tgcttccgga gggcagggac atgggtttgc cgctgaacgg
    11101 ggaaaccata ttgtcgacag agtccgtctg aaaaacgcac ggatcctcgg tgataataat
    11161 gccagaaatg gagcagacag actggtcagc ggaacagaaa tccagacgaa atattgttca
    11221 actgcagccc gtagcgtcgg tgcggcattc gacggacaaa acggacagta tcgttacatg
    11281 ggaaatcatg gtcccatgca actggaagtc ccccgtgatc agtatgccgg cgctgtggaa
    11341 accatgaaga ataagatccg cgaaggtaaa gtacccggtg taaccgatcc cgcagaagcg
    11401 tcccggctga ttcgtcgggg acatctgact tatacccagg cccgtaatat cacccggttc
    11461 gggaccatcg aatcggtcac ttatgatatt gccgaggggt cggttgtcag tctggcggcc
    11521 ggagggatca gttttgccct gacggcatcg gtcttctggc tcagcaccgg cgatcgcgat
    11581 gctgccctgc agacagctgc tgtccaggca ggaaaaacct tcacccgcac actggctgtc
    11641 tacgtcacaa cccagcaact tcaccggctc actgttgttc agggtatgct gaagcatatt
    11701 gatttttcga cggccagccc gactgtccgg caggcgcttc agaaggggac cggtgcagga
    11761 aatatcagtg ccctgaacaa agtgatgaag ggtacgctgg tgacatctct ggcactggta
    11821 gctgtcacaa ccagccctga catgatcaaa atgttgcggg gacggatctc cggtgcgcag
    11881 ttcatcagga atcttgccgt ggcatcttcc ggtgtggcag gtggtgctgt cgggtcagtg
    11941 gcgggcggga tattgttcag tccactggga ccatttggtg cactgacagg gcgtgtggtt
    12001 ggcggtgttc tggggggaat gattgcctcc gctgtatcag gaaaaattgc cggagcgctg
    12061 gttgaagaag atcgcgtcaa aattctggca atgattcagg agcaggtgac atggcttgcc
    12121 ggcagtttcc tgctgaccgg acatgagatt gaaaatctga acgcgaatct ggcccgtgtt
    12181 atcgatcaga atgctctgga gatcattttc gccgccggta tacaacaacg ggccgcgacc
    12241 aatatgttaa tcaaaccact ggtggtcagt atcatcaggc aacgccccgt catggaatat
    12301 gatgcatccc atctcggcaa gatggttaac cggctggaag aagcattccc cccggagctc
    12361 ccggcataac gtgtgttctg ctccttttag gttttatcac ctgatttgcc agtgtcatcg
    12421 tcatgactca gagaggtaaa taccatgatt cacctgttca aaacctgcat gattaccgcc
    12481 ttcattctgg ggttaacgtg gtctgcccca ctccgggcac aggatcaacg ttacatcagt
    12541 atacgcaata cagatacgat atggctcccg ggaaatattt gtgcttacca gttccggctg
    12601 gataatggcg gaaacggtga aggatttggc cccctcacca tcactctgca actcaaagac
    12661 aaatatggtc agacgctggt gaccagaaaa atggaaacgg aagcttttgg tgacagtaat
    12721 gccacgcgaa ccacagacgc atttctggaa acggagtgcg tggaaaatgt cgccacaacc
    12781 gaaatcatta aagcaactga agaaagtaac ggccatcgtg tcagtctgcc gttatcggtt
    12841 ttcgatcccc aggactacca tccactgctg attaccgttt ccggaaaaaa tgttaactga
    12901 tccctgaagt cagtttcatg cttcagcctc tgccacaacg tatggcagag gctatttatc
    12961 aggagaaacc agatatgccg ggttacacac agagtatgtc ctggctgaag gatctttctc
    13021 atacgggcag gcggttgctg tcataaccgc ttaccgcaat gtgtttatcc aggatgaccc
    13081 gggaatgcat ttccgtcggg ttatccgcaa cgcggaaggt cagcgacgct gacgcagccg
    13141 gaactctgaa gctgatgccg gaaaacagct taatgcgtgg ctcgccagtg gagggcttct
    13201 cagacaataa acttcattga tttcatccat caggccgcgt cttctccggg agacgcggct
    13261 tttttcattt gttgcaccag taaatcttaa ccaccgataa ggagcaaagt atgcaattag
    13321 tcagtcgttt tggttatgcg aaccagatac gccgtgaccg tccgctgaca catgaagagc
    13381 tgatgcacca tgtgccgggg attttcgggg aagagaaaca cacgtcccga agccagaact
    13441 atacgtacat ccccaccatc accgtactgg aaagtctgca gcgggaaggc ttccagccct
    13501 ttttcgcctg ccagacccgt gtgcgcgacc cgggccgccg gggatacaca aaacacatgc
    13561 tgcgtctgcg gcgggccgga gagataaacg gagaacatgt ccctgaaatt attctgctca
    13621 actcccatga cggtacctcc agctaccaga tgctgccggg ttacttcaga ttcgtctgcc
    13681 agaacgggtg tgtctgtggt cagtctctgg gggaagtgcg tgttccgcac cggggaaacg
    13741 tggtggagaa agttattgaa ggggcttacg aagtggtggg cgtgtttgac cggattgagg
    13801 agaaacgtga tgccatgcag tcgctggtcc tgccgccacc ggcacgccag gcgctggcac
    13861 aggcggcact gacttaccgt tatggtgacg aacatcagcc cgtcaccacc gccgacattc
    13921 tgacgccacg acgccgggag gattacggta aggacctgtg gagtac
//
LOCUS       DAJTUM010000096        13211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTUM010000096 DAJTUM010000000
VERSION     DAJTUM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OO471_003177"
     CDS             complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OO471_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6244341.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO471_003178"
     CDS             complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO471_003178"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6244342.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2165..3199
                     /gene="rhaT"
                     /locus_tag="OO471_003179"
                     /pseudo
     CDS             2165..3199
                     /gene="rhaT"
                     /locus_tag="OO471_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO471_003180"
     CDS             complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO471_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6244343.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO471_003181"
     CDS             complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO471_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6244344.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5242..6711
                     /gene="rhaB"
                     /locus_tag="OO471_003182"
     CDS             5242..6711
                     /gene="rhaB"
                     /locus_tag="OO471_003182"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6244345.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6708..7926
                     /gene="rhaA"
                     /locus_tag="OO471_003183"
                     /pseudo
     CDS             6708..7926
                     /gene="rhaA"
                     /locus_tag="OO471_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8168..8992
                     /gene="rhaD"
                     /locus_tag="OO471_003184"
                     /pseudo
     CDS             8168..8992
                     /gene="rhaD"
                     /locus_tag="OO471_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9002..9316
                     /gene="rhaM"
                     /locus_tag="OO471_003185"
     CDS             9002..9316
                     /gene="rhaM"
                     /locus_tag="OO471_003185"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6244346.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9617..10063
                     /locus_tag="OO471_003186"
     CDS             9617..10063
                     /locus_tag="OO471_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6244347.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10074..11525
                     /locus_tag="OO471_003187"
     CDS             10074..11525
                     /locus_tag="OO471_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6244348.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11515..12585
                     /locus_tag="OO471_003188"
     CDS             11515..12585
                     /locus_tag="OO471_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6244349.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12585..>13211
                     /locus_tag="OO471_003189"
     CDS             12585..>13211
                     /locus_tag="OO471_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6244350.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQY"
BASE COUNT         3148 a         3450 c         3480 g         3133 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac
      601 cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca
      661 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
      721 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg
      781 atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
      841 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
      901 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
      961 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
     1021 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata
     1081 gacattcatt taatcaataa aagtgatcaa aatcacatca attgaaacgc tgttttattt
     1141 ttataggacc attgttttta ttttggaatt gatcacaaaa aaaccaccgc cgttggcgat
     1201 ggttcatttt ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat
     1261 tattttttcg ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct
     1321 ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
     1381 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
     1441 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
     1501 gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg
     1561 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
     1621 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
     1681 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
     1741 ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg
     1801 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
     1861 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
     1921 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
     1981 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
     2041 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
     2101 agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa
     2161 gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
     2221 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
     2281 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
     2341 ggatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
     2401 cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat
     2461 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
     2521 aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact
     2581 cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga
     2641 gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt
     2701 gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca
     2761 tgaagccgct gccgcacttg gcgtcgatcc actgtatgtt gctctgccaa gctatgttgt
     2821 catcatgggc ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt
     2881 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
     2941 gttactctcg gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg
     3001 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
     3061 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg
     3121 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg
     3181 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
     3241 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
     3301 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
     3361 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
     3421 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
     3481 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
     3541 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
     3601 agccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac
     3661 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
     3721 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
     3781 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
     3841 aatataaaag agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc
     3901 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc
     3961 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
     4021 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
     4081 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc
     4141 cctggcgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
     4201 tactgtcgct gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta
     4261 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg
     4321 tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg
     4381 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
     4441 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
     4501 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
     4561 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag
     4621 acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
     4681 atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca
     4741 gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc
     4801 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
     4861 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag
     4921 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
     4981 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa
     5041 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
     5101 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
     5161 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
     5221 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
     5281 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
     5341 gaaatccatc gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg
     5401 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
     5461 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
     5521 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag
     5581 gcacaacagc aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc
     5641 ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac
     5701 attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac
     5761 tgggaatata ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac
     5821 gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg
     5881 ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
     5941 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
     6001 tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
     6061 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
     6121 ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac
     6181 gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat
     6241 cccaatgacg atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt
     6301 cgggaaacgg cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac
     6361 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat
     6421 ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc
     6481 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat
     6541 atcggcatcg agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
     6601 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac
     6661 tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac
     6721 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
     6781 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
     6841 tttccggttt tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt
     6901 atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc
     6961 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag
     7021 tctcgcgcga ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
     7081 atcagctcgg tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct
     7141 ttacgctctc gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
     7201 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
     7261 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
     7321 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
     7381 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
     7441 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
     7501 cgactgaagt aatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc
     7561 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
     7621 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc
     7681 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg cgttgctgga acctaccgct
     7741 gagctgcgca agctggaagc ggcgggcgat tacactgcgc gtctggcact gctggaagag
     7801 cagaaatcgt tgccgcggca ggcggtctgg gaaatgtatt gccaacatca cgatacgcca
     7861 gcaggtagcg aatggctgga gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt
     7921 gggtaaacac tgccggatgc ggcacgagcg ccttatccgg cctacgggtc gaatgtccat
     7981 gcgggctgtc accctcaccc cggccctctc cctgaaaggg cgagggagaa aaacatagcc
     8041 tcgtgcggca acggagctga acaccgctcc ggacagtccc ctcgccccta cggggagagg
     8101 gttagggtga ggggaacaag ccgcaccatg tctaaacaag gcaacctaat ttacaggaac
     8161 acagaacatg caaaacatta cccagtcctg gtttgtccag ggaatgatca aagccaccac
     8221 cgatgcctag ctgaaaggct gggacgagcg caacggcggc aacctgacgc tgcgcctgga
     8281 tgacgccgat atcgcgcttt atcacgacaa cttccaccca caaccgcgct atattccgct
     8341 cagccaaccc atgcctttac tggcaaatac gccgtttatt gtcaccggct ccggcaaatt
     8401 cttccgtaac gtccagctcg atcctgcagc taacttaggc gtcgtaaaag tcgacagcga
     8461 tggcgcgggc taccatattc tctgggggtt aaccaacgaa gccgttccaa cttctgaact
     8521 tccggctcac tttctctccc actgcgagcg cattaaagcc accaacggta aagatcgggt
     8581 gatcatgcac tgccacgcca ccaacctgat cgccctcacc tatgtactgg aaaacgacac
     8641 cgcggtcttt actcgccaac tgtgggaagg cagcaccgaa tgtctggtag tattcccgga
     8701 tggcgttggc attctgccgt ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc
     8761 acaagagatg caaaaacatt cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg
     8821 accgacgctg gatgaagcct ttggtttaat cgacaccgca gaaaaatcag cacaagtatt
     8881 agtgaaggtt tattcgatgg gcggcatgaa acagaccatc agccgtgaag agttgatagc
     8941 gctcggcaag cgcttcggcg ttacaccact agccagtgcg ctggcgctgt aaggagcaaa
     9001 catgatccgc aaagcctttg tcatgcaggt aaaccccgac gcccacgaag agtatcagcg
     9061 tcggcataat cccatctggc cggaactgga agcagtgttg aaatctcacg gtgtgcataa
     9121 ctacgccatt tacctcgaca aagcgcgtaa tctgctgttt gccatggtag agattgaatc
     9181 tgaagaacgg tggaatgcgg tagccagtac tgatgtttgc caacgttggt ggaaatatat
     9241 gaccgatgtt atgcccgcta acccagataa cagcccggtg agtagcgagc tgcaagaagt
     9301 gttttacctg ccgtaattgc ccaatgtgcc ggagatgcct ccggcacaat attttaaatt
     9361 tcgatttgaa aaattaaaca tattacatct cccggtaagt tatatttccc tgatacattg
     9421 tgagtaaatc acaaaaataa tgaataaccc attaatgatt catgtggttt atttaaataa
     9481 ccattatgtg cattactccg caaatctgac ctttcactct gtctcaaatt gtcattgttt
     9541 accgacatat cgcctctcat cacttaatcg ctccaccatt acaattgatt aaaatatatt
     9601 ttatggagtc cgattaatgg cagctcttac tgcaagctgt attgacctga atattcaggg
     9661 caatggcgct tattccgttc tgaagcagtt ggcgacaata gcgttacaaa acggttttat
     9721 caccgactca caccagttcc tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg
     9781 atttggttcc ggtgtcgcag tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt
     9841 attattcgcc cgcaaagcgc aggctattga ctggaaagcc agcgatggcg aagacgtcaa
     9901 ttgctggatc tgcctcggcg tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg
     9961 cacactgtgt cgcaaaatta ttcaccagga atttattcat caactgcaac agggcgatac
    10021 cgaccaggtg ctggccttgt taaatcaaac cctcagctca taaggaagtg gcgatggagt
    10081 catccttacg tattgtcgcg atcaccaact gccccgccgg gatcgctcac acctacatgg
    10141 tggcggaagc cctggaacag aaagcccgtt ctctcggcca taccataaaa gtggaaactc
    10201 aagggtccag tggcgttgaa aaccgcttat ccagcgaaga gattgccgct gccgattacg
    10261 tcattcttgc taccgggcgt ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga
    10321 aagtttatga gattgccatc tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat
    10381 taccgacaaa ctcgcagctt tttgccgcag atagcggcgt gaagcttggc aaacaggaag
    10441 tgcaaagcgg cagcgtgatg agccacctga tggcgggcgt ctctgcggcg ctgccgtttg
    10501 tcatcggcgg tggtatcctg gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt
    10561 ataccgatat gtcgaaaggc gcgccgtcgt ttacctgggt agttgaatcc atcggttacc
    10621 ttggcttcac ctttatgatc cccatcatgg gcgcttatat tgcctcgtcg attgccgata
    10681 aaccggcttt cgctccggcg tttctggttt gctatctggc gaacgacaaa gcgctgctcg
    10741 gcacccagtc gggtgcggga ttcctcggcg cggtggtgct ggggctggcg attggctatt
    10801 tcgtcttctg gttccgcaaa gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa
    10861 tgctgatccc gttcgttact ctgctggttt tcggcgtgct cacttactac gttatcgggc
    10921 cggtgatgtc cgacctcatg ggcgggctgc tccacttcct gaataccatt cctccatcaa
    10981 tgaagtttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg ggcggcccca
    11041 tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt tatgactggt
    11101 acgccatcgt cggcgttgtc gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca
    11161 ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg
    11221 gtgccacagt cgcaaccgaa ccggctatac cttacgctct ggcagctccg ttgccgatga
    11281 ttaccgccaa tacgctggcg ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca
    11341 aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg tcgccggtag
    11401 gttcgtttta tctggtgctg gcgattggcc tcgcgctgaa catttcattc attatcgtcc
    11461 tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt gtccatgaac
    11521 attgagttac tgcaacagtt gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt
    11581 cgcgacatcc tgataaacac gctggaacct tgcgttaatg aaatcacctt tgatggtctg
    11641 ggcagctttg ttgcccgtaa gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg
    11701 gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg tttcaccacc
    11761 attggtggct ggtggaatca gtcgatgctc aaccatcggg taaccatacg cacacacaag
    11821 ggagtgaaaa tccctggtgt gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa
    11881 aggcaacagc cgctgtcatt tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat
    11941 gaggtggaaa aacgcggtgt ggcgatgggc gattttatta gcccggaagc caattttgcc
    12001 tgctggggcg aagataaagt ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg
    12061 atggctgaac tattgcagac ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc
    12121 gtggaagaag aagttgggct acgcggggca caaacctcgg cggaacacat taaaccggat
    12181 gtagtgatcg tgctggatac cgctgtcgca ggcgatgttc cgggcattga taacattaaa
    12241 tacccgctga aactgggcca ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc
    12301 aaccagaaac tggtagcagc gttaaaaaac tgtgccgcac ataacgattt accgctgcaa
    12361 ttttccacca tgaaaaccgg agcgacggat ggcgggcgct acaacgtgat gggcggcggg
    12421 cgtccggttg ttgcgctgtg cctgccaacc cgctatctgc acgctaacag cggcatgatt
    12481 tcaaaagccg attacgatgc tctgctcacg ttgatacggg gttttctgac gaccttaacc
    12541 gcggagaaag tcaacgcgtt tagccagttc cgtcaggtgg attaatgcta aacgaacgcc
    12601 agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggtgag ctggcgcaac
    12661 agactggcgt ttcaggcagg accatcctgc gtgatattga ctatctcaac tttaccctta
    12721 acggcaaagc ccgcattttc gccagtggca gtgcgggcta tcagctggaa atcttcgagc
    12781 gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg ctggcgctgt
    12841 tattactgaa tactttcact ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa
    12901 cgtgggtagc agagcgtctg ccccagttaa aacagcgtta tgaacgcact tgttgcctgg
    12961 ccagccgccc tggtttgggc catttcattg atgagacaga agagaaactc gttatcttgc
    13021 tggcgaactt gctgcgcaaa gatccgtttt taattccgct ggcgggcata acacgagaca
    13081 accttcagca tttatccacg gcctgcgaca accaacaccg ctggccgctc atacagggtg
    13141 attatctctc cagcctgatt ctggcgattt acgccctgcg taatcaactg accgatgagt
    13201 ggccgcaata t
//
LOCUS       DAJTUM010000097        13177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTUM010000097 DAJTUM010000000
VERSION     DAJTUM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..893)
                     /locus_tag="OO471_003190"
     CDS             complement(<1..893)
                     /locus_tag="OO471_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6244351.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="OO471_003191"
     CDS             1134..1934
                     /locus_tag="OO471_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6244352.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO471_003192"
     CDS             2012..2782
                     /locus_tag="OO471_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6244353.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO471_003193"
     CDS             complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO471_003193"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6244354.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO471_003194"
     CDS             complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO471_003194"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6244355.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5037..5759
                     /locus_tag="OO471_003195"
     CDS             5037..5759
                     /locus_tag="OO471_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6244356.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO471_003196"
     CDS             complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO471_003196"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6244357.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO471_003197"
     CDS             6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO471_003197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6244358.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7152..7228
                     /locus_tag="OO471_003198"
     tRNA            7152..7228
                     /locus_tag="OO471_003198"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7186..7188,aa:Asp,seq:gtc)
     gene            complement(7359..8405)
                     /locus_tag="OO471_003199"
     CDS             complement(7359..8405)
                     /locus_tag="OO471_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6244359.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO471_003200"
                     /pseudo
     CDS             complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO471_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO471_003201"
     CDS             complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO471_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6244360.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO471_003202"
     CDS             complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO471_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6244361.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11365..12195)
                     /locus_tag="OO471_003203"
                     /pseudo
     CDS             complement(11365..12195)
                     /locus_tag="OO471_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12182..13096)
                     /gene="tagK"
                     /locus_tag="OO471_003204"
                     /pseudo
     CDS             complement(12182..13096)
                     /gene="tagK"
                     /locus_tag="OO471_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3030 a         3412 c         3496 g         3239 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc
     3061 gcgcagtttg ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct
     3121 tgaaagcgtg tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc
     3181 aaccagcgtc gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc
     3241 tgcatgcttc tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt
     3301 cacgccagcg ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt
     3361 cgccatttca accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt
     3421 tggcagacgt acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt
     3481 aggcacgtac tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc
     3541 acgcgctttg ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc
     3601 tacggtcaga agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc
     3661 ggcagttgtt gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc
     3721 ataattgcgc cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt
     3781 tgcgtgagga tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt
     3841 acgtttttta acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc
     3901 gtggagatag ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc
     3961 cggaattccc atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat
     4021 agacgtccca tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc
     4081 agctgcagaa aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag
     4141 caggacagag gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa
     4201 gacgaccgat cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct
     4261 ttctttgacc ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta
     4321 attccattaa ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt
     4381 ttcagaatta cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa
     4441 taatcattta tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac
     4501 gtatattcat gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt
     4561 gattgataca tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc
     4621 gtgtcgccgc aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc
     4681 ggtgtggtaa ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact
     4741 acctgtgttg ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac
     4801 ttttccacca gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg
     4861 gcctccggtt gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga
     4921 tcgacaatca ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag
     4981 gcgggaatac tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga
     5041 aaccggcaag agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg
     5101 gagagcttta tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg
     5161 gttttcatct gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg
     5221 tttctcatca agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac
     5281 ttcatcttcc ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt
     5341 ggtgcaccga tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct
     5401 ggctggtgat tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg
     5461 tattgcgcaa aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact
     5521 ggctctcttt gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga
     5581 acaaacacgg aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta
     5641 ttgttgcccg gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca
     5701 agccacgaat tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa
     5761 cttcgacttg gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt
     5821 catcacagcg ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt
     5881 gatgctgccc caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt
     5941 tgtctgctgt tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc
     6001 ggacatactg gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg
     6061 cgacaatagc ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa
     6121 aggttttctc gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac
     6181 ccagacacga accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg
     6241 taattgaatc gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa
     6301 ttacacgcca gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact
     6361 atgaaggcca caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg
     6421 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
     6481 tacatggtat tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
     6541 agtttatgga ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag
     6601 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct
     6661 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc
     6721 tcgatgcgct atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
     6781 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
     6841 tggcttttgc gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca
     6901 ttgtacgtca ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac
     6961 atgaagcccg tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa
     7021 tacatgtaaa aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa
     7081 gatgagaaaa atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg
     7141 gaacacaacg cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc
     7201 gcgggttcga gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg
     7261 ggctttcgtc gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt
     7321 aaaaaatggc cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc
     7381 tccagcagtt ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt
     7441 tctgcctggc gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga
     7501 cggatgacac gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg
     7561 ccggtcatcg gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc
     7621 atctgttcag tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg
     7681 atctccggcg tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg
     7741 gagaagtccg gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg
     7801 gtctctgctg cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc
     7861 agttgctggc gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa
     7921 cgctccacat cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga
     7981 atatccgcca ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc
     8041 gcagcatctt cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg
     8101 tacgtggagc acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc
     8161 tgctggatac ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc
     8221 agacgatggg cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa
     8281 tcgccatatt gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga
     8341 tcgtactcca gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt
     8401 tccatttaat tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgctggactc
     8461 acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct
     8521 ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg
     8581 tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag
     8641 ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc
     8701 gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc cccacagcac gaggctgtac
     8761 agactgatgt gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag
     8821 gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc
     8881 gccggaacgg tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc
     8941 cagggggggg cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc
     9001 tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg
     9061 tattaatgga aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac
     9121 tgatgccgga aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga
     9181 ccagtagtcc ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc
     9241 tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc
     9301 gcaacgcctg gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata
     9361 actggcggat caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg
     9421 gcgggcggat aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc
     9481 ggccattgcg ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga
     9541 tgtggcgcag attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt
     9601 ccgagcccgt atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg
     9661 gctcccggcg cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt
     9721 agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga
     9781 ccgagaacac ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg
     9841 tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac
     9901 agaacaggct gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt
     9961 tcagcaggat aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc
    10021 ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat
    10081 gaccgtgaaa cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt
    10141 acataacggg ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag
    10201 ccaggggcgc gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct
    10261 cgccgcagag ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac
    10321 caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc
    10381 ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg
    10441 gccacagcgt tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa
    10501 tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt
    10561 ccgtatcggg atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc
    10621 tgaccacttc cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg
    10681 tgcgggcggt caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat
    10741 ccgttccctg catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc
    10801 ccgtttcccg cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg
    10861 gtctctctct gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta
    10921 cccggaatgc agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg
    10981 tgttatggcc actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt
    11041 tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact
    11101 tgtgctcata cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg
    11161 ccgcgtacgg ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa
    11221 atgacaaatc ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt
    11281 cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat
    11341 tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca
    11401 ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag
    11461 cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag
    11521 ggagtcggca gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc
    11581 gccattgttc agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg
    11641 cggcgatttc agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat
    11701 cagttcgagt accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc
    11761 attccactgc ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc
    11821 ttcctgcgct tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc
    11881 gggcggcggg agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat
    11941 ctcacagcgg atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg
    12001 ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg
    12061 aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat
    12121 ctgaatgccc tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc
    12181 gttattgttt gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt
    12241 caatgccaat gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg
    12301 tttccggggc gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag
    12361 gccagatagt ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta
    12421 acccaccagt gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat
    12481 ccagatcctg cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat
    12541 cttgttcggg agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc
    12601 ctttttccgc accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat
    12661 tttgtgatgt cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt
    12721 ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct caggttcgga gatgatggat
    12781 tgccatgtac aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac
    12841 gcatacggtg atgaggttcc acaatctggt cgtttaccgt acagcaatat tcggtagact
    12901 gattgataat ccaccatgct tcttcgtggc gggtcagagt gagggcaatc tttacatcat
    12961 cattcacctt aaaaacagga atccagggag aggtaacggt gaagataatt tgtgttcctt
    13021 cagtcgcgta aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg agtccccacg
    13081 taatatttgg tttcattgtg tatagactgg ccccctgaat ctccagacaa ccagtatcac
    13141 ttatttaagt gatagtctta atactagttt ttagact
//
LOCUS       DAJTUM010000098        12908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTUM010000098 DAJTUM010000000
VERSION     DAJTUM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO471_003205"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO471_003205"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6244362.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO471_003206"
     CDS             complement(1806..3224)
                     /locus_tag="OO471_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6244363.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO471_003207"
     CDS             complement(3378..3722)
                     /locus_tag="OO471_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6244364.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO471_003208"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO471_003208"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6244365.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO471_003209"
     CDS             complement(4712..5647)
                     /locus_tag="OO471_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6244366.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO471_003210"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO471_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6244367.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO471_003211"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO471_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6244368.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO471_003212"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO471_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6244369.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO471_003213"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO471_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6244370.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO471_003214"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO471_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6244371.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO471_003215"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO471_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6244372.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO471_003216"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO471_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6244373.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO471_003217"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO471_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6244374.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO471_003218"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO471_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6244375.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3402 a         3299 c         2949 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcacctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAJTUM010000099        12844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTUM010000099 DAJTUM010000000
VERSION     DAJTUM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..346
                     /locus_tag="OO471_003219"
     CDS             <1..346
                     /locus_tag="OO471_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6244376.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO471_003220"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO471_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6244377.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO471_003221"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO471_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6244378.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO471_003222"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO471_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6244379.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO471_003223"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO471_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6244380.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO471_003224"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO471_003224"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6244381.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO471_003225"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO471_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6244382.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO471_003226"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO471_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6244383.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO471_003227"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO471_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6244384.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO471_003228"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO471_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6244385.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO471_003229"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO471_003229"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6244386.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO471_003230"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO471_003230"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6244387.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO471_003231"
     CDS             complement(8961..9236)
                     /locus_tag="OO471_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6244388.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO471_003232"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO471_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6244389.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO471_003233"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO471_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6244390.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO471_003234"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO471_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6244391.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO471_003235"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO471_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6244392.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="OO471_003236"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="OO471_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            11700..>12844
                     /locus_tag="OO471_003237"
     CDS             join(11700..11937,11937..>12844)
                     /locus_tag="OO471_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6244393.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         3077 a         3147 c         3400 g         3220 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat ggtttaaaga gaggtgccca
    11701 tgagcggtaa gcgttatccc gaagagttta aaactgaagc agtcaaacag gttgttgatc
    11761 gcggttattc tgttgccagc gttgcaacac gtctcgatat caccacccac agcctttatg
    11821 cctggataaa gaagtacggt ccggattctt ccactaataa agaacagtca gatgctcagg
    11881 ccgagatccg ccgtctccag aaagagctga aacgggttac cgacgaacgg gacatattaa
    11941 aaaaagccgc ggcgtacttc gcaaagctgt ccgactgagg tacgccttta tccgtgacaa
    12001 ctcctgttgc tggcctgttc gcctgctctg tcgggtgctg gatgttcatc ccagtggttt
    12061 ttacgcctgg cttcagcagc cgcattcaca acgccatcag gcagacctga gactgacagg
    12121 acagattaaa cagttctggc tggaatcggg atgcgtctat ggttatcgca aaatccatct
    12181 ggatctgcgt gacagcgggc aacagtgcgg agtaaacaga gtctggagac tgatgaaacg
    12241 tgtcggaata aaggctcagg tcggataccg aagcccgcgg gcacgtaaag gcgaggccag
    12301 tatcgtgtca cccaacaggc tccagcgaca gttcaatccg gatgctcctg atgagcgttg
    12361 ggtaacggac ataacctaca tcaggaccca cgaaggctgg ctgtatcttg ccgttgttgt
    12421 tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa tcccggatga caaaggacat
    12481 tgtcctgaac gcactgctga tggctgtatg gcggcgtaat cccgaaaaac aggtgctggt
    12541 tcattcggat cagggcagtc agtacacaag ccatgagtgg cagtcgttcc tgaaatcaca
    12601 cggcctggag ggtagcatga gccgtcgcgg taactgccat gataatgcgg ttgcagaaag
    12661 ttttttccag ttgttgaaac gtgaacggat aaagaaaaag atctacggaa cgcgggaaga
    12721 agcccgcagt gatatttttg attacatcga aatgttttat aacagtaagc gtcggcatgg
    12781 ttctagcgaa cagatgtcac cgacagaata tgaaaaccag tattatcaac ggctcggaag
    12841 tgtc
//
LOCUS       DAJTUM010000100        12811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTUM010000100 DAJTUM010000000
VERSION     DAJTUM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1177)
                     /locus_tag="OO471_003238"
     CDS             complement(<1..1177)
                     /locus_tag="OO471_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6244394.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="OO471_003239"
     tRNA            complement(1376..1460)
                     /locus_tag="OO471_003239"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="OO471_003240"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="OO471_003240"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6244395.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="OO471_003241"
     CDS             2804..4306
                     /locus_tag="OO471_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6244396.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO471_003242"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO471_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6244397.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO471_003243"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO471_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6244398.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="OO471_003244"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="OO471_003244"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6244399.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="OO471_003245"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="OO471_003245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6244400.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="OO471_003246"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="OO471_003246"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6244401.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..>12811)
                     /locus_tag="OO471_003247"
     CDS             complement(11871..>12811)
                     /locus_tag="OO471_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6244402.1"
                     /translation="AILMTVSADMPLVGCVLTLLLLVLLIFMAAKGLRYQALMTSLNG
                     VRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGI
                     VSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGY
                     IIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNH
                     FMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTY
                     LLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3243 a         3328 c         3373 g         2867 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg a
//
LOCUS       DAJTUM010000101        12701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTUM010000101 DAJTUM010000000
VERSION     DAJTUM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            758..841
                     /locus_tag="OO471_003248"
     CDS             758..841
                     /locus_tag="OO471_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6244403.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO471_003249"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO471_003249"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6244404.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO471_003250"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO471_003250"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6244405.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO471_003251"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO471_003251"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6244406.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO471_003252"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO471_003252"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6244407.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO471_003253"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO471_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO471_003254"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO471_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6244408.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO471_003255"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO471_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6244409.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO471_003256"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO471_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6244410.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO471_003257"
     CDS             10967..12166
                     /locus_tag="OO471_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6244411.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3122 a         3178 c         3374 g         3027 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgctgc caacttactg
    12661 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct t
//
LOCUS       DAJTUM010000102        12695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTUM010000102 DAJTUM010000000
VERSION     DAJTUM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO471_003258"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO471_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6244412.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO471_003259"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO471_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6244413.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO471_003260"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO471_003260"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6244414.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO471_003261"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO471_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6244415.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO471_003262"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO471_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6244416.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO471_003263"
     CDS             4025..4615
                     /locus_tag="OO471_003263"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6244417.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO471_003264"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO471_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6244418.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO471_003265"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO471_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6244419.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO471_003266"
     CDS             complement(6551..6658)
                     /locus_tag="OO471_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244420.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO471_003267"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO471_003267"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6244421.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO471_003268"
     CDS             7648..7875
                     /locus_tag="OO471_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6244422.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO471_003269"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO471_003269"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6244423.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO471_003270"
     CDS             complement(9447..10703)
                     /locus_tag="OO471_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6244424.1"
                     /translation="MGSNIHGISCTANNYLKHAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO471_003271"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO471_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6244425.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO471_003272"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO471_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6244426.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO471_003273"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO471_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6244427.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3240 c         3198 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggca tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAJTUM010000103        12668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTUM010000103 DAJTUM010000000
VERSION     DAJTUM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(178..897)
                     /locus_tag="OO471_003274"
                     /pseudo
     CDS             complement(178..897)
                     /locus_tag="OO471_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(913..1245)
                     /gene="cusF"
                     /locus_tag="OO471_003275"
     CDS             complement(913..1245)
                     /gene="cusF"
                     /locus_tag="OO471_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6244428.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1269..2651)
                     /gene="cusC"
                     /locus_tag="OO471_003276"
     CDS             complement(1269..2651)
                     /gene="cusC"
                     /locus_tag="OO471_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6244429.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2808..3491
                     /gene="cusR"
                     /locus_tag="OO471_003277"
     CDS             2808..3491
                     /gene="cusR"
                     /locus_tag="OO471_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6244430.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3481..4923
                     /gene="cusS"
                     /locus_tag="OO471_003278"
     CDS             3481..4923
                     /gene="cusS"
                     /locus_tag="OO471_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6244431.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5073..7310
                     /gene="nrfB"
                     /locus_tag="OO471_003279"
     CDS             5073..7310
                     /gene="nrfB"
                     /locus_tag="OO471_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6244432.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7297..10265
                     /gene="nfrA"
                     /locus_tag="OO471_003280"
                     /pseudo
     CDS             7297..10265
                     /gene="nfrA"
                     /locus_tag="OO471_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10266..11156
                     /locus_tag="OO471_003281"
     CDS             10266..11156
                     /locus_tag="OO471_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6244433.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11339..12100
                     /gene="envY"
                     /locus_tag="OO471_003282"
     CDS             11339..12100
                     /gene="envY"
                     /locus_tag="OO471_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6244434.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12146..12222)
                     /locus_tag="OO471_003283"
     tRNA            complement(12146..12222)
                     /locus_tag="OO471_003283"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12186..12188),aa:Arg,seq:tct)
     gene            12465..>12668
                     /locus_tag="OO471_003284"
     CDS             12465..>12668
                     /locus_tag="OO471_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6244435.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3093 a         3294 c         3220 g         3061 t
ORIGIN      
        1 tggtttggta cacatctggt acacaatatc acatggacaa cagcgcacag taaacaacta
       61 tatttgacgt tcgtagacat actcaggtga aaaaaaggtt gtttttcgag ttataacaga
      121 cagttaatta actacagtgg acaatgctag acaatgacag acacagataa gcaacctacc
      181 ttcctctttc gcgaccacgt tatctttggc gatattcatt cccgcgcgca gatcaaacgc
      241 ggtgatcacg ccatcaatgg gcgctttgag cgtaaagcga gtctggattt tttgcgtggc
      301 gatcaggtgg cgaatatccg cctccggcat tcccgccagt cgcagccgct caagaatgcc
      361 ttcagtctgg gtcgccgtac cgccggtttc gcgcaacagt aaatactcac tctgcgcttc
      421 cacccagtcg ggaatggtca ggtcgagaag cggtgtgccc ttttgtactt tatcgcccac
      481 ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga gcctgcacaa tggcatactg
      541 atactcgttg taactgacat tcgtcgggaa actctgggca aaagtcagcg gcccgcgcgt
      601 gacggtagcc gttttcaccc ccagattctg cgtctgagtc gggtcaatgc gcacaccaga
      661 cgcagaactc tcttcatcgg catatttcgg caccagatcc atatccataa acggcgattt
      721 acctggttta tcgaaccgcg tattgggata catcgggtcg taccagaata agattttacg
      781 ttctgcggtc ggcatttttt ctgcgtgcgg ttccgcctgg tcaaaccagg taaaacctgc
      841 cgctgaaata ataccgcccg cgatcatgct gccgataata agcgcgattt ttttcatctc
      901 attaaacctg ggttactggc tgactttaat atcctgtaat aaagaaaggt tgccctgctg
      961 gacaaaatta aacgccactt tgtcgccggt tttaatttca ctcattttcg tctgcggggt
     1021 gatggtaaag cgcatggtca tctccggcca gttgacggca gcaatcggat cgtgatggat
     1081 ggtgattttt ttgctttcca gatcaacacc ttttaccaca ccggtggcac taatgacctg
     1141 tggttgtgct tcgctcatgg tttcatgatg atgttcgtta gcctgggcat taaagccaat
     1201 aacggtaaac agactgaaca ttgcgacttg cagtgctttt ttcatttatt ctctcctgga
     1261 gttaaaaatt attgctgcca accgccccct aaggcggtat acaaataaat ttcgttaacc
     1321 tgacgggcat aattcaggtc aagtaaagtt tgtcgggttg caaataaaga acgctcggca
     1381 tccagcactt ccagataact taccgcgccg tgctgatata atgcccgcgc ccgttgcagg
     1441 gtaatttgca gcgacgccag ataacgctgc tgggcgctga tttgatcgtc caggctttga
     1501 cgtaatgcca gcgcatctgc cacctcttta aaggcgttct ggattttctg ttcataattc
     1561 accaccgact gctgctggcg aatttcggcg atatccagat tggcctggtt gcgtccggca
     1621 ttaaaaatgg ggatctcaat tttaggaata aaattccaca tcccgctgct agcgttaaat
     1681 aatgacgaca gatcgctgct ggcggttgat attccgctgg tcaggctgat agacgggaaa
     1741 aatgccgcac gcgcagcacc aatattagca ttagccgcca ttaacgcgtg ttcagcttcc
     1801 ataatatccg ggcgctgcaa taagatttgc gacgacaagc ccgccggtaa tttaacgctt
     1861 tgcaggctgc cgctgtttac tgtctgcgct tgcggtagct tgccgtagct tcccaataac
     1921 agttgcaatg cattattcgc ctgcgccagt tccccctgac gtttagcgat gtcactgcgg
     1981 gtactttcta tcaccccgcg agcctgttcc agcgccagaa cattgctgct accggtcaac
     2041 agttgttttt cgacaaacgc atatgactgc tgataattac gcagcgtttc ttcggctatt
     2101 tgcaattgcg catacgccag ctgctgattg aaatagcttt gcgcgacatt agaaaccagc
     2161 aaaatatgca ccgcgcgctg ggcttcctca gtggctaaat aattttggcg ctcggcttcg
     2221 ctcatgttct ttaagcgacc gaaaaaatcg agatcaaagc tggcgttaag gccagtcgag
     2281 aactcccgcg tcgtggctga atcgccttta agattgccgc tccagctgcc gctgccctcg
     2341 ccattgagct gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg tgcctcctgc
     2401 actttcagcg tcgccatgcg caaatcccgg ttattcacca gcgcctcgct aatcagcgtc
     2461 ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt tctggtagtt atccgctgcg
     2521 ttaaccaggc cgttctggct gagtgagaac tgctgcggta cgggcattac cggacgctga
     2581 taatccggtg ccagtgaaca accagttagc gcaagggcca cacaaaatgg cagaagttta
     2641 caaggagaca taggctcata atttctggtg attttatgtc gccaacttta ctcgccaggc
     2701 tctgattttc cggtgacagg aaaatgacaa aattgtcatt ttgccaataa gcgattgcca
     2761 tctgatcccg ctactctaga attgcccggg caacatgcgg aggaaatatg aaactgttga
     2821 ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa agggttaacc gaagccggtt
     2881 ttgtggtcga tttggccgac aacgggctga atggctacca tctggcgatg accggtgatt
     2941 atgacctgat aatcctcgat attatgctgc cagacgtgaa cggctgggat atcgtgcgca
     3001 tgttgcgctc cgccaataaa gggatgccga ttctgttgct taccgcgctt ggcaccattg
     3061 aacatcgcgt caaggggctg gagttggggg cagatgacta cctggtgaaa ccgttcgctt
     3121 ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg gtgattatcg
     3181 aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt cagccgcaaa gtcacccgca
     3241 gcggcacgcg catcactctg accagtaaag aatttactct gctggagttt tttctccgcc
     3301 atcagggcga agtgctgccc cgctcgctta tcgcctcgca ggtatgggac atgaatttcg
     3361 acagcgacac taacgccatt gatgtggcgg tgaagctgct acgcggcaaa atcgacaacg
     3421 actttgagcc gaagctgatt caaaccgtgc gcggcgtggg ttacatgctt gaggtgccgg
     3481 atggtcagta agccatttca gcgcccgttt tcgctggcaa cccgcctgac cttttttatc
     3541 agccaggcca ccatcgcggc gtttttcgcc tttgcatgga tcataatcca ctcagtaaaa
     3601 gtgcattttg ccgagcagga cattaatgat ttaaaagaga ttagcgccac ccttgaacgg
     3661 gtactaaatc accctgacga aacgcaagcc cgacgcttaa tgacgctgga agatatcgtc
     3721 agtggttatt ccaacgtgtt gatttccctg gcagatagcc acggcaaaac ggtgtatcac
     3781 tcccccggtg cgcctgatat ccgcgagttt gcacgtgatg ccatacccga taaagacgcg
     3841 cggggtggcg aggtgtatct cctttccggc ccgacgatta tgatgccagg ccacggtcac
     3901 gggcatatgg aacacagcaa ctggcggatg attaacttgc cggttggccc gttggtggac
     3961 ggcaaaccga tttatacgct ctacatcgcg ctgtcgatcg attttcatct tcattacata
     4021 aatgatttga tgaataaact tattatgacc gcatcggtaa tcagcatcct gatcgtcttt
     4081 atcgtgctgt tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag ccgtcaaatc
     4141 cagaatatca cctcgaaaga tctcgacgtt cgccttgacc cgcagaccgt gccgattgag
     4201 ctggaacagc tggtactgtc gttcaaccat atgatcgagc gtattgagga tgtctttact
     4261 cgccagtcca atttctcagc ggatatcgcc cacgaaattc gcacaccaat tactaatctc
     4321 attacgcaaa cggaaatcgc cctcagccag tcgcgcagcc agaaggagct ggaagatgtg
     4381 ctctactcta atctcgaaga gctgacgcga atggcgaaaa tggtcagcga tatgctgttt
     4441 ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa cctggcggat
     4501 gaagtcggca aagtgttcga ttttttcgag gcgctagcgg aagatcgcgg cgtggagttg
     4561 cggtttgttg gcgacaaacg tcaggttgcg ggcgatccgt tgatgctgcg tcgggcgcta
     4621 agcaacctgc tttctaatgc ccttcgttat acgccaacca gagagacaat tgtagtacgc
     4681 tgtcagacgg tcgatcacca ggtgcaagtt tccgtcgaaa accccggtac gcccattgcg
     4741 cccgagcact taccgcgatt gtttgaccgt ttctatcgcg ttgacccctc ccgccagcga
     4801 aaaggtgaag gtagcggtat tgggctggcg atagtgaaat cgattgttgt cgcgcataaa
     4861 ggcacggttg cggtaacgtc agatgcgcgg gggacaaggt ttgttatcac attacccgct
     4921 taattattca gcaaacggca acttttataa ccagtgtaaa aataacgtgc cgcaatagtc
     4981 atacatatta atggtaaaaa gctgtcacaa ttcataaaaa accttaatat acgccaccct
     5041 aaacataacc agcgttaatg taaggttttt gtgtggactg gcttcttgat gtttttgcta
     5101 cctggctcta cggcttaaaa gtaatcgcga taacgttagc ggtcatcatg ttcatcagcg
     5161 ggctggacga tttttttatt gatgtcgtct actgggtacg ccgcattaaa cgcaagttga
     5221 gtgtttatcg ccgctacccg cgaatgagtt accgcgaact gtataaacca gatgaaaaac
     5281 cgttagcgat tatggttccg gcgtggaatg aaacgggcgt catcggcaat atggccgagc
     5341 tggcggcgac cacgctcgac tacgaaaact atcatatctt tgttggcacc taccccaacg
     5401 accccgatac tcaacgtgat gttgacgaag tgtgcgctcg ctttccgaac gtgcataagg
     5461 tagtctgcgc gcgtcctggt cccaccagca aagccgactg cctgaacaac gtgctggacg
     5521 ccatcaccca gtttgaacgt agcgccaatt tcgcttttgc tggttttatt ctgcatgacg
     5581 ccgaagatgt gatttcaccg atggaattgc gtctgttcaa ctatctggtc gagcgtaaag
     5641 atctgattca gatcccagtg tatccgtttg aacgcgaatg gacgcacttc accagcatga
     5701 cttacattga tgagttttca gaactgcatg gcaaagatgt tccggtgcgt gaagccctcg
     5761 ccggacaagt gcccagcgca ggcgtcggca cctgtttcag ccgccgcgcc gtgaccgcac
     5821 tgttagctga cggtaacggt attgctttcg acgtgcagag tcttactgaa gattacgaca
     5881 ttggcttccg cctgaaagaa aaaggtatga cggaaatttt tgtccgtttt ccggtggtgg
     5941 acgaagccaa agaacgcgag cagcgtaaat ttttacagca cgcacggacg tcaaacatga
     6001 tctgcgtgcg cgaatatttc cccgatacct tttcgactgc ggttcgacaa aaatctcgct
     6061 ggatcatcgg cattgttttc cagggcttta aaacccataa atggacctcc agcctgacgc
     6121 tgaactactt tctctggcgc gaccgcaaag gggcaatcag taactttgtc agcttcctcg
     6181 cgatgctggt gatgatccag cttttgctgt tgctggcgta tgaaagtttg tggcccgatg
     6241 cctggcattt cctttctatt tttagcggca gcgcatggtt aatgaccctg ctgtggctaa
     6301 actttggctt gatggttaac cgcatcgtgc agcgagtgat tttcgtcact ggctactacg
     6361 gcctcacgca gggcctgctt tccgtcctgc gtcttttctg gggcaacctg attaacttta
     6421 tggctaactg gcgcgtgctg aaacaggttc ttcagcacgg cgatccgcgt cgcgtcgcgt
     6481 gggataaaac aacgcatgac ttccccagcg tcactggcga tacccgctcg ttgcgcccgt
     6541 taggtcaaat tctgctggaa aatcaggtca tcactgaaga acaactcgat acagcactgc
     6601 gtaatcgcgt cgaaggtcta cgcctgggcg gttcaatgct gatgcagggg ctgattagcg
     6661 ccgagcagct ggcacaggcg ctggcagagc aaaacggcgt ggcgtgggaa tccatcgatg
     6721 cctggcagat cccgtcctcg ctgattgccg aaatgccagc ctccgtagcg ctgcattatg
     6781 cagtactgcc gctgcgtctg gaaaatgatg agttaattgt cggcagtgaa gatggtattg
     6841 acccggtttc gctggcggcc ctgacgcgta aagtcggacg caaagtgcgt tacgtcattg
     6901 ttctgcgggg acaaattgtc acagggttac gccactggta tgcacgccga cgcggtcacg
     6961 atccgcgggc aatgttgtac aatgcggttc agcatcagtg gctcacggaa cagcagaccg
     7021 gtgaaatctg gcggcaatat gtgccgcatc agttcctgtt cgccgaaata ctgaccacgc
     7081 tcggtcatat taatcgttca gcaattaacg tgttgttatt gcgccatgaa cgcagttctc
     7141 tgccgctcgg caagtttttg gtcaccgaag gcgttatcag ccaggaaacg ttggatcgcg
     7201 tcctgacaat tcaacgcgaa ttacaagttt cgatgcaatc actattactc aaagcaggtt
     7261 taaacacaga acaggttgcg caactggagt ccgaaaatga aggagaataa ccttaatcgc
     7321 gtcatcggat ggtctggttt actgctgacg tctttattga gtaccagcgc actcgcagac
     7381 aatatcggca ccagcgcaga agagctgggg ctgagcgatt atcgccattt tgttatttat
     7441 ccccgtctcg ataaggcgct gaaggcacag aaaaataacg acgaagcaac cgccatccgc
     7501 gaatttgaat atatacacca acaggtgccg gataatattc cgctgacttt ataccttgcg
     7561 gaagcctatc gtcattttgg tcatgatgac cgggcgcggc tgttgcttga ggatcaactg
     7621 aaacgtcacc caggagatgc ccgacttgag cgcagtctgg cggctattcc agttgaagtg
     7681 aaaagcgtta cgactgttga agaactgctt gcccagcaaa aagcgggcga cgctgcgccg
     7741 accctgcgtt gtcgcagtga agtcgggcag aatgccctgc ggctggcaca attacctgtc
     7801 gccagagcgc aactgaacga tgcgacgttt gctgcatcgc cggaaggaaa aacgctgcga
     7861 accgatctgc tgcaacgggc aatctacctg aaacaatgat cccaggcaga tacgctatac
     7921 aatgaagcac gccagcagaa cacattaagc gcggcagaac gccgtcagtg gtttgacgtg
     7981 cttcttgccg ggcagctgga cgatcggatc ctggcactgc aatcacaggg gatcttcacc
     8041 gatcctcagt catatattac ttacgcgacc gcgctggctt atcgtggcga aaaagcacgc
     8101 ctccagcatt atctcattga aaataagccg ctgtttacca cgggcgcaca agagaaaagt
     8161 tggctctatc tgttatctaa atacagcgcc aaccccgttc aggcattggc gaattatacg
     8221 gtgcagtttg ccgataaccg ccagtatgtt gttggcgcga cgctaccggt gctgttaaaa
     8281 gaaggtcagt acgacgcagc gcaaaaactg ctcgccaccc tccccgccaa tgaaatgctt
     8341 gaggagcgtt atgctgtcag cgtggcgacc cgtaacaagg ctgaatctct gcgtctggca
     8401 cgattgctgt atcagcaaga accgacaaat cttccccgcc tggatcaact gacctggcaa
     8461 ctgatgcaga acgagcagtc acgcgaagct gccgatttat tgctgcaacg ctatcctttc
     8521 cagggcgatg cgcgtgtcag ccagacttta atggcgcgac tggcgtctct gctggaaagt
     8581 catccttacc tagcaacgcc ggcgaaggtg gcgattttat cgaaaccctt accgctggcg
     8641 gagcaacgtc agtggcaaag tcagttgccg ggtattgcag ataattgccc ggcaatagtt
     8701 cgcttgctgg gcgatatgtc gccttcctac gatgctgccg cttggaaccg tctggcaaag
     8761 tgttatcggg acacgctacc cggtgtggcg ttgtatgcat ggcttcaggc cgaacaacga
     8821 caaccgagcg cctggcaaca tcgtgcggta gcctatcagg cgtatcaggt tgaggactac
     8881 gccaccgcac tggcggcctg gcagaaaatc agtcttcacg acatgagcaa tgatgatctg
     8941 ctcgccgccg ccaacaccgc tcaggcagcg ggaaatggcg cagctcgcga tcgctggctt
     9001 caacaggcag aacaacgtga gctgggaaac aatgctcaat actggtggct gcatgcgcga
     9061 cgttatatcc cagctcagcc agaactcgca ctgaacgatc tcacgcgctc aatcaatatt
     9121 gccccttctg ccaactctta cgttgcgcgg gcgacaattt atcgccaacg tcataatgtt
     9181 ccggccgcgg tgagtgattt gcgcgccgcg ctggaactgg aaccaaataa tagcaacacc
     9241 caggcagcgc ttggttacgc cttgtgggat agcggtgata tcgcacagtc gcgggaaatg
     9301 ctcgaacagg cgcataaagg gcttccggac gatccggcac tgatccgaca actggcctac
     9361 gtgaaccagc gtctggatga catgcctgcg acgcagcact acgcccggct cgtgattgat
     9421 gacattgata atcaggcata accccactga ccccagagca aaatcagcaa cgcttcaatt
     9481 tccgccgttt gcatgaggag gtcggtcgcc gctggacgtt cagtttcgat tcttccatcg
     9541 gcttgcgttc cggcgcaatg agtaccgcta acaataatgt cggcggtgca gcgcctggga
     9601 aaagctatcg tagctacgga caactggaag ccgagtaccg catcggacgc aatatgctgc
     9661 tggaaggcga cctgctctca gtttatagcc gcgtctttgc cgataccgga gaaaacgggg
     9721 tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct gcgctggaag ccgctgcgcg
     9781 atcagatctt tttcctcgcc gtcgaacagc agttgccgct gaacgggcaa aatggcgcat
     9841 ccgataccat gctgcgcgcc agcgcctcat tctttaatgg cggcaaatac agcgacgaat
     9901 ggcacccgaa cggttcaggc tggtttgccc aaaacctgta cctcgatgcg gcgcaatata
     9961 tccgccagga tattcaggcg tggacggcag attatcgcgt cagctggcat cagaaggtag
    10021 ctaacggaca gactattgag ccttacgctc acgttcagga caacggctat cgtgataaag
    10081 gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg gaatatttgg accggcgaga
    10141 cgcactacga cgcctggccg cacaaagtca gtctcggcgt cgagtatcaa cataccttta
    10201 aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt tctcaccatt ggagtgcact
    10261 ggtaaatgcg taagttcatt ttcgtattgc tgacactgct tttggtcagc cctttttcct
    10321 ttgcgatgaa aggtattatc tggcaaccac aaaaccgaga tagtcaggtt accgataccc
    10381 agtggcaggg gctgatgagt cagttacgtt ttcaaggctt cgataccctg gttttgcaat
    10441 ggacccgtta cggcgatgca tttacccagc cagaacagcg cacgttattg tttaagcgtg
    10501 ccgcagctgc gcaacagact ggcctgaagc tgattgtcgg gctgaacgcc gatccggaat
    10561 tttttatgca ccagaaacag tcgtctgcag cgctggaaag ctatcttaat cgcctgctgg
    10621 ctgccgatct ccagcaagcc agattatgga gcgccgcgcc tggcataacg ccggatggct
    10681 ggtacatcag cgcggaaatt gacgacctga actggcgcag cgaagccgcc cgtcaacctt
    10741 tgctaacatg gttaaacaac gcgcagcggc tgattagcga tgtttcagca aaaccggttt
    10801 atatcagtag ttttttcgcc ggaaacatgt cgcccgatgg ctatcgccaa ctgctggaac
    10861 aagttaaagc aaccggagtt aatgtctggg tacaggatgg cagcggcgtg gataaactta
    10921 ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga ttgccaaagc tccgccccag
    10981 caagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg caaaaccttt acagcgaaac
    11041 cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg ttcctcttgc ggtaaagaca
    11101 tgctctattt ctctctgcgc tatttgcccg tcgcgcacgg cattctcgag tattaaatcc
    11161 cctccaggta agtcgggtac gacctggctt acctctttcg ctctcgtcag aattccatca
    11221 agattaattg ctaaaaagca tgctaaagaa agaagatcgt tatcggcgaa cgggccgatc
    11281 actcatgcag gaagcataga tcctttgagc ggagtttctg atatctgagg agtgcgaaat
    11341 gcaattgagc agcagtgaac cttgcgtggt gatcctgacc gaaaaagagg tagaggtaag
    11401 cgtcaataac catgctacgt ttacccttcc gaaaaactac ctggccgcct tcgcgtgcaa
    11461 caataacgtc attgaactct caacgttaaa tcacgtatta atcacccaca tcaaccgtaa
    11521 catcatcaac gattatctgt tgtttttaaa taagaaatta acctgtgtaa agccctggtc
    11581 gcggctagca accccggtta tcgcttgtca tagccgtaca ccggaagtgt tccggctagc
    11641 caccaaccac agcaagcagc aatccagcaa accctgcgaa gcggagttga cgcgcgcatt
    11701 actttttacc gtattgtcta actttcttga gcaatcgcgg tttattgccc tactgatgta
    11761 tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt attcaaagcg atattcagca
    11821 ttactggaat ctgcgaattg tcgccagttc gctatgttta agccccagcc tgctcaaaaa
    11881 gaaattaaaa aacgaaaata ccagctatag ccagattgtc acagagtgtc gtatgcgtta
    11941 cgccgtacag atgttattga tggataacaa aaatatcact caggtggcgc aattatgtgg
    12001 ctatagcagc acgtcgtact ttatctctgt ttttaaggcg ttttacagcc tgacaccgtt
    12061 gaattatctc gccaaacagc gacaaaaagt gatgtggtga agggcaaagc ggaaacggat
    12121 aagacgggca taaatgaaga agaaatggcg cgccctgcag gattcgaacc tgcggcccac
    12181 gacttagaag gtcgttgctc tatccaactg agctaagggc gcgttgatac cgcaatgcgg
    12241 tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca atggcttttg atctggttgc
    12301 tgaacaagtg aacgaccgcg tctgattttc tgatttattt cgctatagcg gcaaacaaac
    12361 gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt catgtatcaa agtacaattt
    12421 accgccctaa cggaaaattg tccgctccta tgagactggt aactatgaaa ccaacgtcgg
    12481 tgatcattat ggatactcat cctatcatca gaatgtctat tgaagttctg ttgcaaaaaa
    12541 acagtgaatt gcagattgtc ctgaaaacgg atgattaccg cataaccatc gattatctcc
    12601 gaacccgtcc tgttgattta atcattatgg atatagactt gcccggaaca gacggtttta
    12661 ccttcctg
//
LOCUS       DAJTUM010000104        12543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTUM010000104 DAJTUM010000000
VERSION     DAJTUM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="OO471_003285"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="OO471_003285"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6244436.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="OO471_003286"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="OO471_003286"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6244437.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="OO471_003287"
     CDS             3116..3400
                     /locus_tag="OO471_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6244438.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="OO471_003288"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="OO471_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6244439.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="OO471_003289"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="OO471_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6244440.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="OO471_003290"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="OO471_003290"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6244441.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="OO471_003291"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="OO471_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6244442.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9586..10686
                     /gene="ompC"
                     /locus_tag="OO471_003292"
     CDS             9586..10686
                     /gene="ompC"
                     /locus_tag="OO471_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6244443.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFIRAVDIRTDNIVATGITYQF"
     gene            10850..11335
                     /locus_tag="OO471_003293"
     CDS             10850..11335
                     /locus_tag="OO471_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6244444.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11445..12434
                     /locus_tag="OO471_003294"
     CDS             11445..12434
                     /locus_tag="OO471_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6244445.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3287 a         3108 c         2925 g         3223 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gaggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgacccc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cctataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tatctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttata ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcacta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcattt cgacttcgat cacatatcga taaataatat tattctattt ttctcattgt
     9481 ttttaatcac cattctattt taaaaacagt atgtcattta taaatgacgc cgtttaaaat
     9541 tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa attaaaaata
     9601 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
     9661 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact
     9721 gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg
     9781 caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac
     9841 gatgaagcca acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt
     9901 agtgaatttg gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca
     9961 tggaccgata tgttgccaga atttggtggc gatacctgga gtcagaaaga tgtcttcatg
    10021 acataccgta ccaccggtct agcaacctat cgcaactacg atttctttgg cttaattgaa
    10081 gggctgaact ttgccgcgca atatcaaggc aaaaataaac gtactgacaa cagtcatctt
    10141 tatggtgctg acgacacgcg tgccaacggt gacggtttcg gtatctcctc aacttatgtt
    10201 tatgatggct ttggtatcgg tgcggtatat accaaatccg atcggacaaa tgcgcaggaa
    10261 agagccgcag ctaatcctct caatgcctcc ggtaagaatg cagaactatg ggccacaggt
    10321 ataaagtatg atgccaacaa catctacttt gcagctaatt acgatgaaac attaaacatg
    10381 accacctatg gggatggtta tatctctaac aaagcacaaa gttttgaagt ggtggcgcaa
    10441 tatcaattcg acttcggctt gcgcccatca ctcgcttacc tgaaatcgaa aggcagagat
    10501 ctgggccgct acgccgacca ggacatgatt gagtatatcg acgttggtgc gacgtatttc
    10561 ttcaacaaaa atatgtcgac ctatgttgat tataaaatca acctgattga tgaaagcgac
    10621 tttatccgtg ccgtagatat tcgcaccgat aacatcgtcg ctacgggcat tacctatcag
    10681 ttctaacgtc ctctcctgac cagcccttct ctgggctggt ttttgattta agtgctgctg
    10741 tatgactcct taagtaaaaa tgagcaattc ttgatatttc tggtcattca cctcaattaa
    10801 tattattcca tatcctccgg ggacatctta ctataatatt tattgatgag tgattatgac
    10861 caatattaat acagcttgtg taaaaaataa tgccagttat caacttaata acgcattacc
    10921 taacaaagaa accatctcca gcaatttttg tgaacgacta gaacaatggg gtaataaatc
    10981 ggttaataat ggtgaagaaa gagcaattgc cgtggagcga attaaagaag cttacaattc
    11041 gaacatggca tctcttgatt tgtcatatct tgatttgagt gaattaccgc ctatcccctc
    11101 gacagttaat acgttaaatc tggaaaacaa ttgtctaact tgtcttgact tagctgacaa
    11161 tgccagtctc gttaatatca acctcagcgt taataaaatt aacacgataa cctttccaaa
    11221 agaaccaaaa ctggaaaaca tttatattaa tcataacaat ctggaaagtt tagatttaaa
    11281 aaatcagcat tcattggtta atctggaagc gcaaaataat agcctaaaaa aatgatattt
    11341 ctgatagtta taaactgaaa tttcttaatc ttgattataa caagctagca tcgctggatg
    11401 tctcccggca agaatctctg attgagttaa gtgcccatca caatatgatc actgacctta
    11461 tattacacaa tcatcccagg atgaaaacaa tcactttaaa cgacaaccat attgcacatt
    11521 taaacgccaa aaacactaca aaactggaat atttaaactt aagcaataac aatttactgc
    11581 caaccaatga cattgatcaa ctaatatcat caaaacatct ttggcatgta ttagttaacg
    11641 gcatcaacaa tgatccactt gcacaaatgc agtactggac tgcagtaaga aatataattg
    11701 atgacactaa tgaagtgacc attgatttat caggacttaa tttaaccact caaccaccag
    11761 ggctgcaaaa cttcacctct atcaatcttg ataataacca actcacacat tttgatgcaa
    11821 ccaactacga tagactcgta aagctaagtc tgaatagtaa tgctcttgag tcaataaatt
    11881 ttcctcaagg cagaaatgta agtattacac atatatctat gaataataat gctctcagaa
    11941 atattgatat agataggctt tcatcagtta cttattttag tgcggcacat aatcaactag
    12001 agtttgtgca attagaatct tgcgaatggc tgcaatacct gaatctcagc cataatcaat
    12061 taactgatat tgttgcaggt aataaaaatg aactcttact gctggatcta tcccataata
    12121 aactaacaag tttacacaat gtcttatttc ccaacttgaa tacgttactt attaacaaca
    12181 atttgctttc tgaaattaaa atattctata gcaacttccg caatgttcag acattaaacg
    12241 ctgctaacaa ccagttgaaa tatataaatc ttgatttcct gacttatctt ccatctatca
    12301 aaagtttaag actggacaat aataaaataa cccacattga tactaataat acatccgata
    12361 ttggaacttt attccccata ataaaacaga gcaaaaactt aaatttttta aatgtttctg
    12421 ggaagaacaa ttgacctact atgcagctca tgttatttaa tttgttttcc ccagcactta
    12481 agcttaatac tggcctggca attctttcgc ctggtgcatt tgaagttcac tctgacggaa
    12541 tag
//
LOCUS       DAJTUM010000105        12536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTUM010000105 DAJTUM010000000
VERSION     DAJTUM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..1040
                     /locus_tag="OO471_003295"
     CDS             <1..1040
                     /locus_tag="OO471_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6244446.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO471_003296"
     CDS             1041..3206
                     /locus_tag="OO471_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6244447.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OO471_003297"
     CDS             3220..4131
                     /locus_tag="OO471_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6244448.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO471_003298"
     CDS             4131..5426
                     /locus_tag="OO471_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6244449.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO471_003299"
     CDS             5471..5701
                     /locus_tag="OO471_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244450.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO471_003300"
     CDS             5679..6179
                     /locus_tag="OO471_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6244451.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO471_003301"
     CDS             6179..7597
                     /locus_tag="OO471_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6244452.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO471_003302"
     CDS             7597..8445
                     /locus_tag="OO471_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244453.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO471_003303"
     CDS             8445..8900
                     /locus_tag="OO471_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6244454.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO471_003304"
     CDS             8903..9595
                     /locus_tag="OO471_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6244455.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO471_003305"
     CDS             9605..10936
                     /locus_tag="OO471_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6244456.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12536
                     /locus_tag="OO471_003306"
     CDS             10937..>12536
                     /locus_tag="OO471_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6244457.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRN"
BASE COUNT         3365 a         3107 c         3304 g         2760 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gaagcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatg
//
LOCUS       DAJTUM010000106        12237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTUM010000106 DAJTUM010000000
VERSION     DAJTUM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..446
                     /locus_tag="OO471_003307"
     CDS             <1..446
                     /locus_tag="OO471_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6244458.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            462..938
                     /locus_tag="OO471_003308"
     CDS             462..938
                     /locus_tag="OO471_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6244459.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1001..1222
                     /locus_tag="OO471_003309"
     CDS             1001..1222
                     /locus_tag="OO471_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6244460.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1385..1759
                     /locus_tag="OO471_003310"
     CDS             1385..1759
                     /locus_tag="OO471_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6244461.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1806..2180
                     /locus_tag="OO471_003311"
     CDS             1806..2180
                     /locus_tag="OO471_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6244462.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2177..2668
                     /locus_tag="OO471_003312"
     CDS             2177..2668
                     /locus_tag="OO471_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244463.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2680..2877
                     /locus_tag="OO471_003313"
     CDS             2680..2877
                     /locus_tag="OO471_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244464.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2962..3522
                     /locus_tag="OO471_003314"
                     /pseudo
     CDS             2962..3522
                     /locus_tag="OO471_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3594..4598
                     /locus_tag="OO471_003315"
     CDS             3594..4598
                     /locus_tag="OO471_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6244465.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            4880..5161
                     /locus_tag="OO471_003316"
                     /pseudo
     CDS             4880..5161
                     /locus_tag="OO471_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(5311..5398)
                     /locus_tag="OO471_003317"
     tRNA            complement(5311..5398)
                     /locus_tag="OO471_003317"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5362..5364),aa:Ser,seq:gga)
     gene            5633..6571
                     /gene="ghrA"
                     /locus_tag="OO471_003318"
                     /pseudo
     CDS             5633..6571
                     /gene="ghrA"
                     /locus_tag="OO471_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6626..7363
                     /gene="ycdX"
                     /locus_tag="OO471_003319"
     CDS             6626..7363
                     /gene="ycdX"
                     /locus_tag="OO471_003319"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6244466.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            7387..7941
                     /gene="ycdY"
                     /locus_tag="OO471_003320"
     CDS             7387..7941
                     /gene="ycdY"
                     /locus_tag="OO471_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6244467.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            8043..8534
                     /locus_tag="OO471_003321"
     CDS             8043..8534
                     /locus_tag="OO471_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6244468.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(8667..9373)
                     /locus_tag="OO471_003322"
                     /pseudo
     CDS             complement(8667..9373)
                     /locus_tag="OO471_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(9400..9816)
                     /gene="csgF"
                     /locus_tag="OO471_003323"
     CDS             complement(9400..9816)
                     /gene="csgF"
                     /locus_tag="OO471_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6244469.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9841..10230)
                     /gene="csgE"
                     /locus_tag="OO471_003324"
     CDS             complement(9841..10230)
                     /gene="csgE"
                     /locus_tag="OO471_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6244470.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(10235..10885)
                     /gene="csgD"
                     /locus_tag="OO471_003325"
     CDS             complement(10235..10885)
                     /gene="csgD"
                     /locus_tag="OO471_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6244471.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            11638..12093
                     /gene="csgB"
                     /locus_tag="OO471_003326"
     CDS             11638..12093
                     /gene="csgB"
                     /locus_tag="OO471_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6244472.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            12134..>12237
                     /locus_tag="OO471_003327"
     CDS             12134..>12237
                     /locus_tag="OO471_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6244473.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         3198 a         2889 c         2982 g         3168 t
ORIGIN      
        1 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccactccc gtccctgatg
       61 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg
      121 aaccccgtgt cttcggctgg atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga
      181 atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg
      241 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
      301 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt
      361 atgccatgac ggtccattat taccggctgc gggattacgc cctgcagcat ccggaatgca
      421 gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag ctttcctttc
      481 tgcccggaaa catgacgccc ggcgagcgca gtctcattct gcgggccctg caaaccctgg
      541 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca cgggaatggc
      601 tgattctgaa catggcggga ctggagcggg aagagttccg ggtgctgtat ctgaacaacc
      661 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgt acggaagttc
      721 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc
      781 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
      841 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
      901 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc
      961 tgttttcact tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg
     1021 tgaagccatg cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg
     1081 taagtatcgc tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc
     1141 cggcgttctt gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct
     1201 gatgagtgtc accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc
     1261 tcagacgccg ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt
     1321 cagcgcgccg gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt
     1381 tatcgtgtca gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg
     1441 gtgggggctt ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa
     1501 ccgcctgtat taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc
     1561 ataccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     1621 cggtgaactg aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     1681 cgaagccgac accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc
     1741 acccgcaacc acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac
     1801 ttaagatgaa aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg
     1861 tcaccatctg gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga
     1921 atgacacacc gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     1981 tgtgtgatgc agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2041 gattcagcac ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc
     2101 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     2161 agtatccgga ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt
     2221 acaggcactg aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact
     2281 gattaacatg gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac
     2341 ctcagcagaa tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca
     2401 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg
     2461 tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc
     2521 atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt
     2581 cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct
     2641 gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa
     2701 acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt
     2761 atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag
     2821 gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc
     2881 tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat
     2941 ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac
     3001 gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt
     3061 acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa
     3121 agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc
     3181 tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa
     3241 atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt
     3301 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt
     3361 ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc
     3421 aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt
     3481 aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc
     3541 caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga
     3601 acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca
     3661 agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg
     3721 cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc
     3781 gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt
     3841 ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac
     3901 gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac
     3961 accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt
     4021 tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc
     4081 tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata
     4141 cccactactt ttaccttgtt gagcaaatag cagagctgga atcagaatta agtcagtcaa
     4201 tcaacgctga tgataccgct cagcgcatta tgacaattcc tggtgttggt ccgattactg
     4261 ccagcttact atcgtcgcaa cttggtgacg ggaaacagtt ctcatgcagc agggattttg
     4321 cagcttccac aggacttgtt ccccggcagt atagcactgg aggaaaatcg actctgttgg
     4381 gtatcagtaa gcgaggtgat aaaaatttac gccggttact ggttcagtgt gcccgctcat
     4441 tcataatgcg acttgaacgc aagcatggaa aacttgctga gtgggttcgg gagcaactca
     4501 acaagaagca ttcgaatgtg gttacctgcg ctctggcaaa taaactggct cgaattgcct
     4561 gggcaatcac tactcagaaa aaagaatatc aggcctgata tagatgctga tataactcaa
     4621 ttagtttatt ttaaacaata agatacaagt tacctttctg gttttgcgaa gactgattat
     4681 tgatgacatg aacggcaaat cgacctgatt aaaaacctaa cagatgaaat ggctcactga
     4741 agccgagggg atttttagga ttatcaggtg cggaactcat cggggcgcgg gcatccttgc
     4801 ccacagagac gccggataga tttaagcaag ccaaccatta atcaaaaatc agtgttgcaa
     4861 aaacgggggt gaccatagat ttcatcttat caccggacaa caggctgacc gactcgccga
     4921 tctggaaaga atgttgcacc tgttcagcgg taaaccaatc cccgacaacc ggggaaacat
     4981 cacccttcgt ctggatgatc acattcagtc tgttcagggt aaagagagct atgaagatga
     5041 gatgttcagc atcagatact ttaagaaggg ctccgcgcat atcacgttca gaaaaccaga
     5101 actggttgac agactgaatg atattatcgc aaaacactat cctgatatgc tggcagtata
     5161 gctttgccag tatgctcaaa gatcggaaat tgtccgataa gatatgatcg cggggcatat
     5221 aaaccggttt cagatggcat acccgatggc atactgtctg gaaggaaaaa taaaaaagcg
     5281 catgttaaac aataacatgc acttataatc tggcggtgag ggggggattc gaacccccga
     5341 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
     5401 attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg gaaatcatga cctgaggtca
     5461 acaaactttt tgaaaaaatc gcgcgtttat tcaaacattc aatcaatgtg tggttttaat
     5521 aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg gcctttgtag
     5581 atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca gtatggatat
     5641 catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc gcaaagctat
     5701 tcctcaggca agagtcaggg catggaaaag cggagataat gactctgctg attatgtttt
     5761 agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg tgttcgcact
     5821 cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa tgctgaaccc
     5881 ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc aggaatatgc
     5941 tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc agcaaaatag
     6001 ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg gcattttggg
     6061 cgcaggcgta ctgggctgta aagttgctca gagtctgcaa acctagcgct ttccgctgcg
     6121 ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg gacgggaaga
     6181 actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga atacccctga
     6241 aaccgtcggc attattaatc aacaattact cgaaaaatta cctgatggcg cgtatctcct
     6301 caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc tggatagcgg
     6361 gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc ctgaaagtcc
     6421 gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta cccgtcccgc
     6481 tgaagctgtg gagtacattt ctcgcactat tgcccagctc gaaaaagggg agagggtctg
     6541 cgggcaagtc gaccgcgcac gtggctacta ataaagcatc aggattcctg ctatccttgg
     6601 cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc ataccgttgc
     6661 cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac aaaagggcat
     6721 taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc atcactggca
     6781 cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc tgcgcggcat
     6841 tgaagctaac attaaaaatg ttgatggtga aattgactgc agcggtaaaa tgtttgactc
     6901 gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg acaaagcgac
     6961 caatacacaa gcgatgatct ccactatcgc cagcggcaat gtgcatataa taagccatcc
     7021 cggaaatccg aaatatgaaa tagatgttaa agccgttgct gaagcagccg cgaaacatca
     7081 ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca gtgaagacaa
     7141 ctgtcgtgcg gtagctgcag cggtacgtga tgctggaggt tgggtggcat taggctcgga
     7201 ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt
     7261 agattttccg ctagagcgca ttttgaatgt ttctccgcgc cgcttactga acttccttga
     7321 atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag taatggaaat
     7381 atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa
     7441 ccgcaagatc ctttactggt gccgctgttt actctgattc gtgaggggaa actggctgcg
     7501 aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg tgatatgacg
     7561 caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt gccgccatat
     7621 cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct ttccgaacga
     7681 gggatgctac tggcggatac gccagccgat cacatcggca cattgctgct tgcggcttcc
     7741 tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact gttcagtgag
     7801 tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc aaccacgcct
     7861 ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg ggacgagctg
     7921 gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac agataatttg
     7981 ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct atcatgcgcg
     8041 gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt atctggggat
     8101 gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt acggcctact
     8161 ttgcttgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg
     8221 gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat ctggaaattc
     8281 tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc aaacagctgc
     8341 tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc gggcagggtg
     8401 actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac gcaatgaaaa
     8461 acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag caggaaatca
     8521 aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaatgccg gattattaat
     8581 tccggcattt ttatctgtca ggattccggt ggaaccgaca tatggcggta tttcaccaga
     8641 atgtcattct gccgttctgc tttattttac aaatcccaca gaccacggtc gataccatca
     8701 ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat aacgggttcg
     8761 tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa gcggaaaacc
     8821 ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga gaggatctca
     8881 ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg gtattgcgtg
     8941 tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt gctttcataa
     9001 ccgataatcg aaccttcaac cataatattt gccgccgtta aagattgcag cgggattcgg
     9061 ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taaccttgcg ctcgttaagc
     9121 aggttttgta agccctggcg ctttgcggaa cagcagtgga gaagttactt gccgggtagg
     9181 gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc ttacccgtcg
     9241 gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat gtcggtctgg
     9301 cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca accaaaagaa
     9361 ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg ttgagttatt
     9421 ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg tcacgttcaa
     9481 ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca ccatgcggcc
     9541 cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact ggatggcctg
     9601 agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat agctcggatc
     9661 tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat ttgggttacc
     9721 accaaagttt ggattacgga actggaaagt catggttcca gcccaactta atggcgaaat
     9781 aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac aattttttat
     9841 ttagaattca tcatgcgcca aatcgtccgt acttaataac gcctgattta tctggcgacg
     9901 atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcgg agtctctttt
     9961 caacgggaat aaaaaagtct ggaaaataac atcctgattg accgttattg tgatccagct
    10021 tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt cactttccca
    10081 tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat ggtcagttaa
    10141 caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca gaaattctgc
    10201 cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta tcgtttgccc
    10261 aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta taaagatgcg
    10321 ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta gacgcgccga
    10381 tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat tccgtgctgt
    10441 tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc gtaaagtagc
    10501 attcgccacg caggacgcct tgcaacccat tgacaacacg ttcttgatcc tccatggcat
    10561 aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa tcttcaggcg
    10621 tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc tgccaataat
    10681 ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct gaagagatat
    10741 cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgctagcgat tgtttaaggt
    10801 gctgcaagag agctgtcgcc tgcaaagaag gtttagtgat caacaataat gtatgaccat
    10861 gaatactatg gacttcatta aacatgatga aaccccactt ttttattgat cgcacacctg
    10921 acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact gctgtgtgta
    10981 gtaataaatc agccctaaat gggtaaaata taaaactaat ggattacatc tgatttcaat
    11041 ctagccatta caaatcttaa atcaagtgtt aaacatgtaa caaaatgtaa ctcattatat
    11101 taaaatgtta acattaaggt tttattaagt ttagaaatga tagaaaagtt gtacatttgg
    11161 tttttattgc acaattttaa aaaatcatac aaatggtgct aacttactaa taatacatat
    11221 aaaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt tctccacacc
    11281 tccgtggaca attttttact gcaaaaagac gaggtttgtc acggcttgtg cgcaagacat
    11341 atcgcagcaa tcagcgacgg gcaagaagaa tgactgtctg gtgatttttg atagcggaaa
    11401 acggagattt aagagaaaac aaaatatttt ttgcgtagat aacagcgtat ttacgcgggt
    11461 tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag ttattaaaaa
    11521 tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag gttgcgttaa
    11581 caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg tgacaacatg
    11641 aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat tgcagccgca
    11701 gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt gagtaagtct
    11761 tcatttaatc aggcagccat aattggtcag gctgggacta ataatagtgc tcagttacgg
    11821 cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa tcgggcaaag
    11881 attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag tgccaacgat
    11941 gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa aggttctggt
    12001 aataaagcaa atattacaca gtatggtact caaaaaacgg cagttgtagt gcagagacag
    12061 tcgcaaatgg caattcgcgt gacacaacgt taattaccat tcgactttta aatcaatccg
    12121 atgggggttt tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg tattctccgg
    12181 tagcgctctg gcaggtgttg ttcctcagta cggcggcggc ggtaaccacg gtggtgg
//
LOCUS       DAJTUM010000107        12219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTUM010000107 DAJTUM010000000
VERSION     DAJTUM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..492)
                     /locus_tag="OO471_003328"
     CDS             complement(<1..492)
                     /locus_tag="OO471_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6244474.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO471_003329"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO471_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6244475.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO471_003330"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO471_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6244476.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO471_003331"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO471_003331"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6244477.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO471_003332"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO471_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6244478.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO471_003333"
     CDS             3646..4155
                     /locus_tag="OO471_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6244479.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO471_003334"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO471_003334"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6244480.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO471_003335"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO471_003335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6244481.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO471_003336"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO471_003336"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6244482.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO471_003337"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO471_003337"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6244483.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO471_003338"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO471_003338"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6244484.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO471_003339"
     CDS             complement(8797..9690)
                     /locus_tag="OO471_003339"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6244485.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO471_003340"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO471_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO471_003341"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO471_003341"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6244486.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="OO471_003342"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="OO471_003342"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6244487.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJTUM010000108        12111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTUM010000108 DAJTUM010000000
VERSION     DAJTUM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..369
                     /locus_tag="OO471_003343"
     CDS             <1..369
                     /locus_tag="OO471_003343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244488.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO471_003344"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO471_003344"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6244489.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO471_003345"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO471_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6244490.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO471_003346"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO471_003346"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6244491.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO471_003347"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO471_003347"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6244492.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO471_003348"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO471_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6244493.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO471_003349"
     CDS             complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO471_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6244494.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO471_003350"
     CDS             complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO471_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6244495.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO471_003351"
     CDS             complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO471_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6244496.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO471_003352"
     CDS             complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO471_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6244497.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO471_003353"
     CDS             complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO471_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6244498.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO471_003354"
     CDS             complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO471_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6244499.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11911..>12111)
                     /locus_tag="OO471_003355"
     CDS             complement(11911..>12111)
                     /locus_tag="OO471_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6244500.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2782 a         3387 c         2934 g         3008 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgaac cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagag aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgccaggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agttaacgct
     6361 gtgtaatagt ttttatattt ttcatcaaaa ttacggctgg tagcgaccat ttcaggtaac
     6421 ggtgccatgc ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg
     6481 ctatcgagca attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc
     6541 accgctgaac gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc
     6601 tcgccctgct gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg
     6661 gaagaaaaaa acaaactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt
     6721 accaacagcg tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg
     6781 atctcgttat cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg
     6841 aaataaaaaa cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat
     6901 tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt
     6961 tcgatgttca ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct
     7021 gttgaaagcg tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt caatgaaagc
     7081 acgtctgaaa cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata
     7141 actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca
     7201 atcggcacaa taacgccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca
     7261 atccgtgtta cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac
     7321 tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt
     7381 gttacattcg tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg
     7441 ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc
     7501 tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa
     7561 ggtttttaac cacaacctgg tgttgaccaa ttaattgatc caccagcaag gcgtagcggc
     7621 gaccgccact ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga
     7681 cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact tccagcaccc
     7741 gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat tccataacag
     7801 cattcagcgg cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg
     7861 ccagcgtcag cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt
     7921 cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc
     7981 cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcattgcaa
     8041 cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa
     8101 ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct
     8161 gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt
     8221 ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa
     8281 tgcgttctat caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct
     8341 gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt
     8401 ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta
     8461 actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct
     8521 gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct
     8581 tttctaccgc tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca
     8641 cgcggccggt tggcgcttgt tcggctgcca gtttaagcac aggtggggta gctatttttg
     8701 gcgaaacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg
     8761 ctgtgatgtc atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt
     8821 tcaccacgtc atttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc
     8881 cggccttcag gcgcgaaagg ataattcgtc gcggcaactg actgcgactt tgctcatctt
     8941 gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg
     9001 cttctaatgc cagttgacgc aaggcctggc agatataatc gtagctggcg gcatccggct
     9061 cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca
     9121 gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt
     9181 ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga
     9241 tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca
     9301 aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct
     9361 gataaaaatc gcttatatcg atgctcacgc tgtcacctcg gttcggctga tggcattgtg
     9421 ggaacactga cctgtggtgc aacctcaggt ttttctaggg cgcttactgg ctcattctgg
     9481 ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac caacaggctg
     9541 atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg
     9601 ccgacgacac gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat
     9661 gcattggccc gatcggcaga aagctcccag ttgctgtaac ctttctcacc gctggcgtag
     9721 ggaaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagcacaggc
     9781 gcgatggcgc gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac
     9841 atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt
     9901 ttgagatgga gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca
     9961 ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc
    10021 tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt
    10081 tcactattgg aaatgcgatc gccgcccgta accgctgtcg ccagtggagt ccggaagtac
    10141 tccgcaatct gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa
    10201 aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc
    10261 ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg
    10321 cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt
    10381 caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag
    10441 gcggtgcgta accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc
    10501 tggtttcggc gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca
    10561 ataaaatacc gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg
    10621 caggacgatc ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa
    10681 acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt
    10741 cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc
    10801 tgataatcag gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc
    10861 gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa
    10921 acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat
    10981 acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt
    11041 taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca
    11101 ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga
    11161 aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact
    11221 gttgaccatg acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg aaaaaaaaag
    11281 cagcggtacg tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct
    11341 gtactctctg ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac
    11401 gtctttttac tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct
    11461 ggtgagcgtg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc
    11521 cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagt ggtccttctt
    11581 ctgcttgtgg gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat
    11641 tacacaaacc ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt
    11701 tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc
    11761 ttccgcgcag ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt
    11821 ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg
    11881 cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc
    11941 aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg
    12001 gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg
    12061 gaagtgacaa accagctgat tggtttctgc cagcttaacc atttgcggaa g
//
LOCUS       DAJTUM010000109        11925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTUM010000109 DAJTUM010000000
VERSION     DAJTUM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..2342)
                     /locus_tag="OO471_003356"
     CDS             complement(<1..2342)
                     /locus_tag="OO471_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6244501.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO471_003357"
     CDS             complement(2276..2899)
                     /locus_tag="OO471_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6244502.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO471_003358"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO471_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO471_003359"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO471_003359"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6244503.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO471_003360"
     CDS             7507..8229
                     /locus_tag="OO471_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6244504.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO471_003361"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO471_003361"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6244505.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OO471_003362"
     CDS             complement(10301..11896)
                     /locus_tag="OO471_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR6244506.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3308 c         2945 g         2915 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcgg
//
LOCUS       DAJTUM010000110        11717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTUM010000110 DAJTUM010000000
VERSION     DAJTUM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            129..998
                     /locus_tag="OO471_003363"
     CDS             129..998
                     /locus_tag="OO471_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6244507.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1201..1554)
                     /locus_tag="OO471_003364"
     CDS             complement(1201..1554)
                     /locus_tag="OO471_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6244508.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1692..3338)
                     /gene="groL"
                     /locus_tag="OO471_003365"
     CDS             complement(1692..3338)
                     /gene="groL"
                     /locus_tag="OO471_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6244509.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3382..3675)
                     /locus_tag="OO471_003366"
     CDS             complement(3382..3675)
                     /locus_tag="OO471_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6244510.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3951..5207
                     /gene="yjeH"
                     /locus_tag="OO471_003367"
     CDS             3951..5207
                     /gene="yjeH"
                     /locus_tag="OO471_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6244511.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5223..5699)
                     /gene="fxsA"
                     /locus_tag="OO471_003368"
     CDS             complement(5223..5699)
                     /gene="fxsA"
                     /locus_tag="OO471_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6244512.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6036..7472
                     /gene="aspA"
                     /locus_tag="OO471_003369"
     CDS             6036..7472
                     /gene="aspA"
                     /locus_tag="OO471_003369"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6244513.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7590..8891
                     /gene="dcuA"
                     /locus_tag="OO471_003370"
     CDS             7590..8891
                     /gene="dcuA"
                     /locus_tag="OO471_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6244514.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9007..9345
                     /gene="cutA"
                     /locus_tag="OO471_003371"
     CDS             9007..9345
                     /gene="cutA"
                     /locus_tag="OO471_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6244515.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9321..11018
                     /gene="dsbD"
                     /locus_tag="OO471_003372"
     CDS             9321..11018
                     /gene="dsbD"
                     /locus_tag="OO471_003372"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6244516.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11055..11630
                     /locus_tag="OO471_003373"
     CDS             11055..11630
                     /locus_tag="OO471_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6244517.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2814 a         3025 c         2881 g         2997 t
ORIGIN      
        1 cttttctgtt tatatagagt gagcacggct atttatgtta ttttaacaac tattactacc
       61 aagcccattt atctggctta taaattaaaa ttaattattt aattgagaaa tggttaggga
      121 gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata
      181 atttcattgc actggcgctg aaaattaaag aacctcgcaa taaagaatcg ttgttcttta
      241 tgtctgtcat ggaactgcgc gacctgctga ttgcgctgga aagtcgtctg catcaaaagc
      301 ataaactgga tgctgctgca cgccttcagt atgagcaagc tcgcgataaa gtcattaaga
      361 aaatggcaga aaacatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc
      421 gtcgggttaa cacgctggaa cttaccgata atcaaggcga aaatcttacc ttcgtattaa
      481 cgctacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg
      541 cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta
      601 cctcactgtt agatttcctg ccgttatacg acgttgactg ccaggacaat ggcaatctgg
      661 aatatgacac ctattcccaa ccggaatgga aacataactt gttcgaccat tatctggcgg
      721 tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa
      781 cgcgtgaagc aacgccgggt aaagaaatag aggcgatcac tcgccgaatg cttgatttca
      841 gcccacgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg
      901 cgaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg
      961 tacaatccac cagcaaaact gcaccacagg cgaaataatc tcatctttgc taatgcgagg
     1021 ctggagccat ccagcctgaa atgactgata acgtgatcgt tggtcatgcc ggatgcggcg
     1081 taaacgcctt atccggccta caaattctca agcattcaat cagttgcaat gcctgctgta
     1141 ggcctgataa gcgaagcgca tcaggcaata atacgtttgt cacccagcct ctggatcaca
     1201 tcagttcggg cacttataaa cctgaccgat aatctggcta tccgtcggga caaaactgga
     1261 caacattccc tgcgacgggc tgctgatgcc ataaatcacg ttaccgccca ttgcagccgc
     1321 ctggttgcgc agatcgtttg ctgcgccgcg catagaaccg ccctcttctc cgtgttgccc
     1381 ggaaagccag ttgctttgct tacctgtcgc agtaccaatc agctggcact ctgcgcctgg
     1441 ctgctcgtcc acaatgcgta cactctgacc ggcagcactc aattcgttgc tggagctaca
     1501 acccgccatc agtagcgcgg caccgacaat ccctgctaag tattttacgt gcatgttatt
     1561 ccccataatc aatgagctgg acgccactcg cgtcatccgt gtctgaatct tatactagaa
     1621 agatgaccaa aagaaaaacc cccagacatt tctgcccggg ggtttgttta tttctgcgaa
     1681 gtgcagggca attacatcat gccgcccatg ccacccatgc cgcccatacc gccagcagcg
     1741 cctaagtcag ctgcatcgtt tttcggcagg tcggtaacca tgcattcggt ggtgatcatc
     1801 aggccagcca cagaagctgc gtactgcaga gcagaacggg ttactttggt tgggtccagg
     1861 atacccatgt cgatcatgtt gccgtattct tcggttgctg cgttgtaacc gtagttgccg
     1921 tcgccgcctt taacggtgtt agcaacaaca gacggttctt cgccgcagtt caggacgatc
     1981 tgacgcagcg gagcttccat tgcacgcagt gcaactttga tacccacgtt ctggtcttcg
     2041 ttctgaccac gcaggtcagc cagtttagac gctacgcgga tcagcgcaac accaccacca
     2101 gcaaccacgc cttcttctac cgcagcacgg gtcgcgtgca gggcatcttc aacgcgtgct
     2161 tttttctctt tcatttcaac ttcggtagca gcacctactt tgataactgc aacgccgcct
     2221 gccagtttcg ctacgcgctc ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca
     2281 atctgctgac ggatctgagc aacacggccc tggattgcag cttcttcacc cacgccatcg
     2341 atgatagtgg tggtgtcttt gttgatcaca acacgtttag cctgacccag gtcttccagg
     2401 gttgcttttt ccagctccat accgatctct tcagagatta cggtaccgcc agtcagggtt
     2461 gcgatatcct gcagcatagc tttacgacga tcgccgaagc ccggagcttt aaccgcagca
     2521 actttcacga tgccacgcat ggtgttaaca accagagttg ctagcgcttc gccttcaaca
     2581 tcttcagcga tgatcagcag cggtttgcct gctttggcaa cagcttccag aaccggcagc
     2641 atttcgcgga tgttggagat tttcttgtca gccagcagga tgaacgggct ttccagttct
     2701 actgcgccag tttccggctt gttgatgaag taaggagaca ggtagccacg gtcgaactgc
     2761 ataccttcaa ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg
     2821 ccttctttac cgactttgtc catcgcttca gcgatcagtt tacctacggt ttcgtcggag
     2881 ttagcggaga tggtaccaac ctgagcaatc gctttagaat cagagcacgg tacggacagc
     2941 gctttcagtt cttcaactgc agcggtaacc gctttgtcga taccacgttt caggtccatc
     3001 gggttcatgc ccgcagcaac agctttcaga ccttcagtga tgatagcctg agccagtacg
     3061 gttgcagtgg tggtaccgtc gcctgcagcg tcgttcgctt tggaggcaac ttctttcacc
     3121 atctgcgcac ccatattttc gaacttgtct tccagttcga tttcacgagc aacggaaaca
     3181 ccatctttgg tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt acggcctttt
     3241 ggaccgaggg taactttcac tgcatctgcc agtacgttta cgccgcgcag cattttcaca
     3301 cgagcgtcgt taccgaattt tacgtcttta gctgccatta tctttattcc ttaaattcgt
     3361 atgttcagtg tcgtgcgcgg attacgcttc aacaattgcc agaatgtcgc tttcggacat
     3421 gatcaacact tcttcattgt cgatcttctc ggatttcaca ccgtagccat cgttgaaaat
     3481 aacgatgtcg ccaactttca cgtccagcgg cttcacttcg ccattttcaa ggatacggcc
     3541 attgccgaca gccagcactt cgccgcgggt ggatttagcc gctgcagagc cggtcagaac
     3601 gatgccgcca gcagatttag tttcaacttc tttacgcttg acgatcacgc gatcatgcaa
     3661 tggacgaata ttcattgata actctccttt gagaaagtcc gtatctgtta tgggtgacgc
     3721 cggagcttac gtggtttccc ggctggtgac cagagaaatg gggatgaggc ttcgcccctt
     3781 caagggggaa aaagaaaaaa aattctgatc agcacaaaat cgggtgaaaa ccctgattca
     3841 cctcacattt catcgcaatt tcttcatcgc cgtcataagc gaatctgatt gtgctaccat
     3901 cgaaaatcta cgcagttgcc caaaatttgg gcgcaatcgg atatcaaccc atgagtggac
     3961 tcaaacaaga actggggctg gcccagggca ttggcctgct atcgacgtca ttattaggca
     4021 ctggcgtgtt tgccgttcct gcgttagctg cactggtagc aggcaataac agcctgtggg
     4081 cgtggcccgt tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc
     4141 gccactatcc cagcgcaggc ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc
     4201 ttgagcgagt caccggctgg ctgtttttat cggtcattcc cgtgggtttg cctgccgcac
     4261 tacaaattgc cgccggattc ggccaagcaa tgtttggctg gcatagctgg caactgttgt
     4321 tggcagaact cggtacgctg gcgttggtgt ggtatatcgg tactctcggt gccagttcca
     4381 gtgctaatct acaaaccgtt attgccggac ttatcgtcgc actgattgtc gctatctggt
     4441 gggcgggcga tatcaaacct gcgagtatcc ccttccccgc gccaggaaat atcgaactta
     4501 ccgggttatt tgctgcgtta tcagtgatgt tctggtgttt tgtcggtctg gaagcatttg
     4561 cccatcttgc ctcggaattt aaaaatccag agcgtgattt tcctcgtgct ttgatgattg
     4621 gtctgctgct ggcaggatta gtctactggg gctgtacggt agtcgtctta cacttcgacg
     4681 cctatggtga acaaatggcg gcggcagcat cgcttccaaa aattgtagta cagttgttcg
     4741 gtgtaggagc gttatggatt gcctgcgtaa ttggctatct ggcctgcttt gccagtctta
     4801 acatttatat acagagcttc gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc
     4861 actacctggc acgcctctct tctcgccata tcccgaataa tgccctcaat gcggtgcttg
     4921 gctgctgcgt ggtgagcacg ttggtaattc atgccttaga gatcaatctg gacgctctta
     4981 ttatttatgc caatggcatc tttattatga tttatctgtt atgcatgctg gcaggctgta
     5041 aattattgca aggtcgttat cgactactgg cggtggttgg cgggctgtta tgcgttctgt
     5101 tactggcaat ggtcggctgg aaaagtctct atgcgctgat catgctggtg gggttatggc
     5161 tgtttctgcc aaaacgaaaa acgccggaaa atggcataac cacataatcc ggcgtttcga
     5221 cattaatcct ggcgatcgtc tttatgatca aggcggtcgc ggtcatcatc ctttcgctga
     5281 tactcaccat caaaagtatt accgccaccg gtcccggcgc taaaaccgcc gcctggcatg
     5341 cgagaaaagc gcaaatgtgg catcaacttc actgtcagat gcttttgcac cggcggcaat
     5401 aaaagcagaa gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag caaaccggca
     5461 ataatcagcg aaacactttt aatcatctcc gccgcagggt tttcacccgc cgccattttt
     5521 tgctgcatca gcacgaaatt cttaaagccc tggttacgta ccagtgacat accgataact
     5581 gacgtgaata taaccagcac gagggtcagc aataccccca atacatgggc aacctgaata
     5641 aagattgaaa tctcaatata gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa
     5701 ggtttctcct gtaatagcag ccggttaaac ccggctacct gaatgggttg cgaatcgcct
     5761 tcagcttata ttgtggtcat tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag
     5821 taatcccaaa gcggtgatct atttcacaaa ttaataatta aggggtaaaa accgacactt
     5881 aaagtgatcc agattacggt agaaatcctc aagcagcata tgatctcggg tattcggtcg
     5941 atgcagggga taatcgtcgg tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa
     6001 agcaaatcat tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag
     6061 aagatctgtt gggtaccagg gaagttccag ctgatgccta ctatggtgtt cacactctga
     6121 gagcgattga aaacttctat atcagcaaca acaaaatcag tgatattcct gaatttgttc
     6181 gcggtatggt aatggttaaa aaagccgcag ctatggcaaa caaagagctg caaaccattc
     6241 ctaaaagtgt agcgaatgcc atcattgccg catgtgatga agtcctgaac aacggaaaat
     6301 gcatggatca gttcccggta gacgtctacc agggcggcgc aggtacttcc gtaaacatga
     6361 acaccaacga agtgctggcc aatatcggtc tggaactgat gggtcaccag aaaggtgaat
     6421 atcagtacct gaacccgaac gaccatgtta acaaatgtca gtccactaac gacgcctacc
     6481 cgaccggttt ccgtatcgca gtttactctt ccctgattaa gctggtagat gcgattaacc
     6541 aactgcgtga aggctttgaa cgtaaagctg tcgaattcca ggacatcctg aaaatgggtc
     6601 gtacccagct gcaggacgca gtaccgatga ccctcggtca ggaattccgc gctttcagca
     6661 tcctgctgaa agaagaagtg aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta
     6721 accttggcgc aacagcaatc ggtactggtc tgaacacgcc gaaagagtac tctccgctgg
     6781 cagtgaaaaa actggctgaa gtcactggct tcccatgcgt accggctgaa gacctgatcg
     6841 aagcgacctc tgactgcggc gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg
     6901 tgaagatgtc caaaatctgt aacgacctgc gcttgctctc ttctggtcca cgtgccggcc
     6961 tgaacgagat caacctgccg gaactgcagg cgggctcttc catcatgcca gctaaagtaa
     7021 acccggttgt tccggaagtg gttaaccagg tatgcttcaa agtcatcggt aacgacacca
     7081 ctgttaccat ggcagcagaa gcaggtcagc tgcagttgaa cgttatggaa ccggtcattg
     7141 gccaggccat gttcgaatcc gttcacattc tgaccaacgc ttgctacaac ctgctggaaa
     7201 aatgcattaa cggcatcact gctaacaaag aagtgtgcga aggttacgtt tacaactcta
     7261 tcggtatcgt tacttacctg aacccgttca tcggtcacca caacggtgac atcgtgggta
     7321 aaatctgtgc cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga
     7381 ctgaagcgga acttgacgat attttctccg tacagaatct gatgcacccg gcttacaaag
     7441 caaaacgcta tactgatgaa agcgaacagt aatcgtacag ggtagtacaa ataaaaaagg
     7501 cacgtcagat gacgtgcctt ttttcttgtg agcagtaact taaaaataac aatctaatat
     7561 caacttgtta aaaaacaagg aaggctaata tgctagttgt agagctcatc atagttttgc
     7621 tggcgatctt cttgggcgcc agattggggg gaataggtat tggttttgca ggcggattgg
     7681 gggtgctggt tcttgccgct attggcgtta aacccggtaa catcccgttc gatgtcattt
     7741 ccattatcat ggcggttatc gccgctattt ctgccatgca ggttgctggc ggtctggact
     7801 atctggttca tcagacagaa aagctgctgc gccgtaaccc gaaatacatc acgatcctcg
     7861 caccgatcgt gacctatttc ctgactatct ttgctggtac tggcaacatc tctctggcga
     7921 cactgccagt tatcgctgaa gttgcgaagg aacaaggcgt taaaccttgc cgtccgctct
     7981 ctactgcagt ggtatccgcg cagattgcga tcaccgcatc gccaatctca gcggcagtgg
     8041 tttacatgtc ttccgtgatg gaaggtcatg gcatcagcta cctccatctg ctctccgtgg
     8101 tcatcccgtc caccctgctg gcggttctgg tgatgtcctt cctggtcact atgctgttca
     8161 actccaaact ctctgacgat ccgatttatc gcaagcgtct ggaagagggc ttggttgaac
     8221 tgcgcggtga aaagcagatt gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc
     8281 tgctgggcgt agttggcgtg gttatctatg caatcatcaa cagcccaagc atgggtctgg
     8341 ttgaaaaacc gctgatgaac accaccaacg caatcctgat catcatgctc agcgttgcaa
     8401 ctctgaccac cgttatctgt aaagtcgata ccgacaacat cctcaactcc agcaccttca
     8461 aagcaggtat gagcgcctgt atttgtatcc tgggtgttgc gtggctgggc gatactttcg
     8521 tttccaacaa catcgactgg atcaaagata ccgctggtga agtgattcag ggtcatccgt
     8581 ggctgctggc cgtcatcttc ttctttgctt ctgctctgct gtactctcag gctgcaaccg
     8641 caaaagcact gatgccgatg gctctggcac tgaacgtttc accgctgacc gctgttgctt
     8701 ctttcgctgc ggtgtctggt ctgttcattc tgccgaccta cccgacgctg gttgctgcgg
     8761 tacagatgga tgacacgggt actacccgta tcggtaaatt cgtcttcaac catccgttct
     8821 tcatcccggg tactctgggt gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct
     8881 tcatgctgta atgatcaatc gcggggcgtt cacgccccgc tttctttccc gccgactaac
     8941 atccttcccc cgtccgttgt atagtgacct ctctcttgcg gttccatctg ttcttgcgag
     9001 gtgtttatgc ttgatgaaaa aagttcgaat accacgtctg tcgtggtgct atgtaccgca
     9061 ccggatgaag cgacagccca ggatttagcc gccaaagtgc tggcggaaaa actggcggcc
     9121 tgcgcgacct tgatccccgg cgctacctct ctctattact gggaaggtaa gctggagcaa
     9181 gaatacgaag tgcagatgat tttaaaaact accgtatctc accagcaggc actgctggaa
     9241 tgcctgaagt ctcatcatcc atatcaaacc ccggaacttc tggttttacc tgttacacac
     9301 ggagacacag attacctctc atggctcaac gcatctttac gctgattctg ctactttgca
     9361 gcacttccgt ttttgccgga ttattcgacg cgccgggacg ttcacaattt gtccccgcgg
     9421 atcaagcctt tgcttttgat tttcagcaaa accaacatga ccttaatctg acctggcaga
     9481 tcaaagacgg ttactacctc taccgtaaac agatccgcat tacgccggaa cacgcgaaaa
     9541 ttgccgacga gcagctgccg caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg
     9601 agatttaccg cgatcggctg acgcttcccg taaccatcaa ccaggcgagt gcgggagcga
     9661 cgttaactgt cacctaccag ggctgtgctg atgccggttt ctgttatccg ccagaaacca
     9721 aaaccgttcc gttaagcgaa gtggtcgcca acaacgcagc gtcacagcct gtgtctgttt
     9781 cgcagcaaga gcagcacacc gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg
     9841 gtattggtat cgcctttacg ccatgcgtgc tgccaatgta cccactgatt tctggcatcg
     9901 tgctgggtgg taaacagcgg ctctccactg ccagagcatt gttgctgacc tttatttatg
     9961 tgcaggggat ggcactgact tacacggcgc tgggtctggt agttgccgcc gcagggttac
    10021 agttccaggc ggcgctacag cacccatacg tgctcattgg cctcgccatc gtctttacct
    10081 tgctggcgat gtcaatgttt ggcttgttta ctctacaact cccctcttcg ctgcaaacac
    10141 gtctcacgtt gatgagcaat cgccaacagg gcggctcacc tagcggtgtg tttgttatgg
    10201 gggcgattgc cggactgatc tgttcaccat gcaccaccgc accgcttagc gcgattctgc
    10261 tgtatatcgc ccaaagcggg aacatgtggc cgggcggcgg cacgctttat ctctatgcgt
    10321 tgggcatggg cctgccgctg atgctaatta ccgtctttgg taaccgcttg ctgccgaaaa
    10381 gcggcccgtg gatggaacaa gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg
    10441 tcttcctgct ggagcgagtg attggtgatg tatggggatt acgcttgtgg tcggcgctgg
    10501 gtgtcgcatt ctttggctgg gcctttatca ccagcctaca ggctaaacgc ggctggatgc
    10561 gcgtggtgca aataatcctg ctggcagcgg cattggttag cgtgcgccca cttcaggatt
    10621 gggcatttgg tgcgacgcat accgcgcaaa ctcaaacgca tctcaacttt acacaaatca
    10681 aaacggtaga tgagttaaat caggcgctcg ttgaagccaa aggcaaaccg gtgatgttag
    10741 atctttatgc cgactggtgc gtcgcctgta aagagtttga gaaatacacc ttcagcgacc
    10801 cgcaggtgca aaaagcgtta gcagacacgg tcttacttca ggccaacgtc acggccaacg
    10861 acgcacaaga tgtggcgctg ttaaagcatc ttaatgtcct tggcctaccg acaattctct
    10921 tttttgacgg acaaggccag gagcatccac aagcacgcgt cacgggcttt atggatgctg
    10981 aaaccttcag cgcacatttg cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga
    11041 cggaggaaaa tagcgtgcaa cgtgaagatg tactgggaga agccctgaaa ttattagaat
    11101 tacaagggat tgccaacacc acgctggaga tggttgctga acgtgtggat tatccactgg
    11161 acgagctacg ccgcttctgg ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc
    11221 ttagccaaca gatagatgtc tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg
    11281 aacaaaagct gctggcacgt tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc
    11341 cgggctgtct atttatcgct gcctgtacgt tttatcccga tcctggccac cctattcatc
    11401 aactggccga tcagcaaaaa agcgctgcct acgatttcac ccacgaactg ttaaccacgc
    11461 tggaagttga cgatccggcg atggtagcaa agcagatgga actggtgctg gaaggctgtt
    11521 taagccgaat gctggtgaat cgtagccagg cggatgtcga cactgcacat cggctggcgg
    11581 aagatatcct gcgcttcgcc cgctgccgcc agggtggtgc attgacctga cagaaacaca
    11641 gaaaagaagc gatttgccgc aatcttaagc agttgaatcg cttttactga aattaggttg
    11701 acgagatgtg cagatta
//
LOCUS       DAJTUM010000111        11658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTUM010000111 DAJTUM010000000
VERSION     DAJTUM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            597..794
                     /locus_tag="OO471_003374"
     CDS             597..794
                     /locus_tag="OO471_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244518.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO471_003375"
     CDS             complement(744..1937)
                     /locus_tag="OO471_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6244519.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO471_003376"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO471_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6244520.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO471_003377"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO471_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6244521.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO471_003378"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO471_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6244522.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO471_003379"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO471_003379"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6244523.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO471_003380"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO471_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6244524.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO471_003381"
     CDS             complement(10573..10716)
                     /locus_tag="OO471_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244525.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11658
                     /locus_tag="OO471_003382"
     CDS             10955..>11658
                     /locus_tag="OO471_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6244526.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2956 a         2899 c         2992 g         2811 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactc
//
LOCUS       DAJTUM010000112        11592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTUM010000112 DAJTUM010000000
VERSION     DAJTUM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="OO471_003383"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="OO471_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6244527.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="OO471_003384"
     CDS             complement(2051..2974)
                     /locus_tag="OO471_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6244528.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="OO471_003385"
     CDS             3191..4534
                     /locus_tag="OO471_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6244529.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="OO471_003386"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="OO471_003386"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6244530.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="OO471_003387"
     CDS             6554..6778
                     /locus_tag="OO471_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6244531.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="OO471_003388"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="OO471_003388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6244532.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="OO471_003389"
     CDS             complement(7372..8352)
                     /locus_tag="OO471_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244533.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="OO471_003390"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="OO471_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6244534.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="OO471_003391"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="OO471_003391"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6244535.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11017
                     /locus_tag="OO471_003392"
     CDS             10361..11017
                     /locus_tag="OO471_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6244536.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="OO471_003393"
                     /pseudo
     CDS             complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="OO471_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3002 a         2705 c         2905 g         2980 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta
    11401 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
    11461 gactaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
    11521 tttcacagac caaagtatca tttgacctgt gaaacgatta caggcattta aattccagtt
    11581 aagaccaggt gg
//
LOCUS       DAJTUM010000113        11588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTUM010000113 DAJTUM010000000
VERSION     DAJTUM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(282..357)
                     /locus_tag="OO471_003394"
     tRNA            complement(282..357)
                     /locus_tag="OO471_003394"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO471_003395"
     CDS             916..3168
                     /locus_tag="OO471_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244537.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4436
                     /gene="csiD"
                     /locus_tag="OO471_003396"
     CDS             3504..4436
                     /gene="csiD"
                     /locus_tag="OO471_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6244538.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4456..5724
                     /gene="lhgO"
                     /locus_tag="OO471_003397"
     CDS             4456..5724
                     /gene="lhgO"
                     /locus_tag="OO471_003397"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6244539.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5747..7195
                     /gene="gabD"
                     /locus_tag="OO471_003398"
                     /pseudo
     CDS             5747..7195
                     /gene="gabD"
                     /locus_tag="OO471_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7203..8489
                     /gene="gabT"
                     /locus_tag="OO471_003399"
                     /pseudo
     CDS             7203..8489
                     /gene="gabT"
                     /locus_tag="OO471_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8727..10127
                     /gene="gabP"
                     /locus_tag="OO471_003400"
     CDS             8727..10127
                     /gene="gabP"
                     /locus_tag="OO471_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6244540.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10148..10810
                     /gene="csiR"
                     /locus_tag="OO471_003401"
     CDS             10148..10810
                     /gene="csiR"
                     /locus_tag="OO471_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6244541.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO471_003402"
     CDS             complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO471_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6244542.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11344..11502)
                     /locus_tag="OO471_003403"
     CDS             complement(11344..11502)
                     /locus_tag="OO471_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6244543.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2980 a         2846 c         2889 g         2873 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 gggggaagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg
     4081 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
     4141 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
     4201 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
     4261 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     4321 tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     4381 tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc
     4441 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     4501 accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag
     4561 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     4621 tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc
     4681 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct
     4741 cagctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     4801 cgcgagtgga taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt
     4861 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca
     4921 aaaatcttcc aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt
     4981 gagcataaaa acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg
     5041 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     5101 ggctttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     5161 attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat
     5221 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     5281 cgcgaaggct atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg
     5341 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     5401 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     5461 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag
     5521 ctgattgacg attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc
     5581 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     5641 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     5701 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     5761 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     5821 tgaagtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     5881 gggcgcagat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg
     5941 tgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca
     6001 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc
     6061 gaaaggcgaa atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg
     6121 tatttatggc gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca
     6181 gccgattggc gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg
     6241 taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac
     6301 gccgttctct gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt
     6361 gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct
     6421 ggtacgcaaa ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg
     6481 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     6541 tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc
     6601 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     6661 tgccgaaaaa ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg
     6721 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     6781 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg
     6841 caacttcttc cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga
     6901 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     6961 acaagccaat gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg
     7021 cgtcttccgc gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat
     7081 ttccaatgaa gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     7141 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     7201 gaatgcgaag gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     7261 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     7321 tttaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     7381 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     7441 tccaggtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     7501 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     7561 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt
     7621 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     7681 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     7741 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     7801 aagatatcgc cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     7861 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     7921 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     7981 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg
     8041 gcgtcaccgg gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct
     8101 atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg
     8161 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga
     8221 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg
     8281 agctgtttga agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg
     8341 cacgcgcccg cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     8401 gcatccttgt accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg
     8461 gtcagtgttt tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc
     8521 gtcttgtccg gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc
     8581 tgtacgtgaa caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa
     8641 aagaatcaat aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa
     8701 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg
     8761 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     8821 tgtttgtcgg ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc
     8881 tgttcgccgg attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca
     8941 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct
     9001 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca
     9061 tcgccgccat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     9121 tcattaccct cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     9181 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     9241 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     9301 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     9361 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa
     9421 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     9481 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     9541 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     9601 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     9661 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     9721 aaaccccgta cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga
     9781 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     9841 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     9901 aaggaagcga aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg
     9961 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
    10021 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
    10081 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
    10141 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
    10201 cggtaatttt caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact
    10261 tggcgttggg ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt
    10321 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
    10381 cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga
    10441 tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
    10501 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
    10561 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
    10621 ggcgcgttat cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga
    10681 taaacacgat cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc
    10741 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
    10801 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
    10861 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
    10921 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
    10981 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
    11041 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
    11101 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
    11161 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga
    11221 gtttttctcc ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg
    11281 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
    11341 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
    11401 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat
    11461 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
    11521 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
    11581 gccagttt
//
LOCUS       DAJTUM010000114        11351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTUM010000114 DAJTUM010000000
VERSION     DAJTUM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO471_003404"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO471_003404"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6244544.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO471_003405"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO471_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6244545.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OO471_003406"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OO471_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6244546.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OO471_003407"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OO471_003407"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6244547.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="OO471_003408"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="OO471_003408"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6244548.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="OO471_003409"
     CDS             complement(5839..6879)
                     /locus_tag="OO471_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6244549.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="OO471_003410"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="OO471_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6244550.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="OO471_003411"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="OO471_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6244551.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="OO471_003412"
     CDS             7821..8261
                     /locus_tag="OO471_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6244552.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="OO471_003413"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="OO471_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6244553.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="OO471_003414"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="OO471_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6244554.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11351
                     /gene="rsxC"
                     /locus_tag="OO471_003415"
     CDS             9490..>11351
                     /gene="rsxC"
                     /locus_tag="OO471_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6244555.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2651 a         2927 c         2865 g         2908 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat
    11221 gcggaaccag aagaacaggt cgatccgcgc aaagccgccg tcgaagccgc tattgcccgt
    11281 gccaaagcgc gcaagctgga acagcaacag gctaatgcgg aaccagaaga acaggtcgat
    11341 ccgcgcaaag c
//
LOCUS       DAJTUM010000115        11111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTUM010000115 DAJTUM010000000
VERSION     DAJTUM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO471_003416"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO471_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6244556.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO471_003417"
     tRNA            complement(621..695)
                     /locus_tag="OO471_003417"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO471_003418"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO471_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO471_003419"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO471_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6244557.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO471_003420"
     CDS             complement(2932..3990)
                     /locus_tag="OO471_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244558.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO471_003421"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO471_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6244559.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO471_003422"
     CDS             6068..6352
                     /locus_tag="OO471_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6244560.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO471_003423"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO471_003423"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6244561.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO471_003424"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO471_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6244562.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO471_003425"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO471_003425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6244563.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2544 c         2748 g         3044 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacttggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt c
//
LOCUS       DAJTUM010000116        11049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTUM010000116 DAJTUM010000000
VERSION     DAJTUM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            410..1240
                     /locus_tag="OO471_003426"
     CDS             410..1240
                     /locus_tag="OO471_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6244564.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="OO471_003427"
     CDS             complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="OO471_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6244565.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2369..3829)
                     /locus_tag="OO471_003428"
     CDS             complement(2369..3829)
                     /locus_tag="OO471_003428"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6244566.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="OO471_003429"
     CDS             complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="OO471_003429"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6244567.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5434..6777
                     /gene="gntP"
                     /locus_tag="OO471_003430"
     CDS             5434..6777
                     /gene="gntP"
                     /locus_tag="OO471_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6244568.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="OO471_003431"
     CDS             complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="OO471_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6244569.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="OO471_003432"
     CDS             complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="OO471_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6244570.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="OO471_003433"
     CDS             complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="OO471_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6244571.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9006..>11049)
                     /gene="fimD"
                     /locus_tag="OO471_003434"
     CDS             complement(9006..>11049)
                     /gene="fimD"
                     /locus_tag="OO471_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6244572.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2685 a         2829 c         2683 g         2852 t
ORIGIN      
        1 tacgtagatg aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt
       61 ccgcataatc agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa
      121 catatcaaat acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc
      181 aatatcaaat tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa
      241 tgagcgtttt ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga
      301 tatcactaca acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat
      361 ttatctattt ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc
      421 attaagcaat gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa
      481 agtcgacagt gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta
      541 tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat
      601 tagaaaactt aatattggca aacagtcctg gccatttaat tatgtgacag atacatgcag
      661 agaattcaat ggtattacaa caacaggacg tatgccctat cgaaacttga aaatcacctc
      721 agctttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc
      781 agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac
      841 agttcatgaa ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat
      901 aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag
      961 aattgatatc tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt
     1021 cgcccttgcc gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat
     1081 agaagcctat cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg
     1141 tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt
     1201 tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac
     1261 taatattcag gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat
     1321 tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag
     1381 tcaggcgtcg actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc
     1441 gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc
     1501 cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
     1561 caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg
     1621 attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt
     1681 tgcttcagca atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct
     1741 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
     1801 ggtagcctgc agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa
     1861 cagctcaaaa ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct
     1921 gcctgagcta tcaagcacat agataccggc accccggcgc acttccacca gccctttgat
     1981 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
     2041 ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgtg
     2101 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt
     2161 gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg
     2221 cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag
     2281 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga
     2341 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     2401 gttcgcacag ctgctgatat gccgacgtca ccgcgccaac aaagtcggca ttctgcggca
     2461 gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat
     2521 actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac
     2581 cctgctcatc cacgccctgg gtgtaaccca tccagccagc cacgcccagc gccaggtgac
     2641 gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact
     2701 tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac
     2761 gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg
     2821 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg
     2881 tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc
     2941 cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgtgc
     3001 ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga
     3061 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     3121 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt
     3181 tctcttcaat ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga
     3241 ccttcgccac atggccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca
     3301 gccctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg
     3361 cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg
     3421 ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac
     3481 gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca
     3541 tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca
     3601 gttgctgttt tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt
     3661 cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt
     3721 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     3781 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     3841 gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc
     3901 cggtgggtat taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     3961 ggccaggcct ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc
     4021 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     4081 tttacgacgg tgttcttctt caacaatcgc tttcaccact ttgtacatat caacgtcacc
     4141 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     4201 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     4261 acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc
     4321 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg
     4381 cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat
     4441 cgctttcagg aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag
     4501 ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc
     4561 aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc
     4621 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg
     4681 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     4741 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     4801 gcgaatgcca cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc
     4861 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca
     4921 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     4981 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     5041 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     5101 tcctctttca catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt
     5161 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     5221 tctacctttt ggtaagacat gtcaaccaga attctgaaat tggttaaccg cgtcgcaaga
     5281 atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     5341 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     5401 taacaacggt tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     5461 gtattcggca ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg
     5521 gctttgttgg tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg
     5581 ctgcacacca tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg
     5641 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat
     5701 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctt
     5761 attttcggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt
     5821 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     5881 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct
     5941 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta
     6001 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     6061 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     6121 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     6181 gtaaaagata cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg
     6241 tttattgcga tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag
     6301 tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc
     6361 ggtgcgggtg gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc
     6421 atgttgatgt cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta
     6481 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt
     6541 gctgcaatcc ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta
     6601 ctggcgacgg ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg
     6661 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     6721 ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag
     6781 aacaccagag cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg
     6841 gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg
     6901 cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg
     6961 tgcaggtttt gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt
     7021 aattttttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     7081 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta
     7141 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     7201 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     7261 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     7321 gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     7381 catcacaacc gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact
     7441 gaaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac
     7501 cagctttaat agccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7561 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7621 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag
     7681 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7741 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt
     7801 ttacataaac attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac
     7861 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag
     7921 ctcgtttcat tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7981 gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg
     8041 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     8101 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     8161 taatatccgg tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     8221 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata
     8281 agactgaaag aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac
     8341 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     8401 cttgcgagcg ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc
     8461 tccagttact gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca
     8521 ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc
     8581 agggtcgtcc acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca
     8641 ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg
     8701 ttggcattgt ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta
     8761 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     8821 aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca
     8881 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg
     8941 cgacttaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     9001 cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc
     9061 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     9121 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     9181 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc
     9241 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga
     9301 gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta
     9361 tccagcgcca ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc
     9421 cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     9481 acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat
     9541 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata
     9601 ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta
     9661 ccatcgcctc ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc
     9721 aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     9781 tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa
     9841 ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     9901 aggctatagc tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat
     9961 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
    10021 gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg
    10081 aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
    10141 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa
    10201 taacggtaac ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga
    10261 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
    10321 atatccacag acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca
    10381 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
    10441 agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct
    10501 cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag
    10561 ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca
    10621 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
    10681 taaatgccat atccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg
    10741 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
    10801 gcgccgcgaa agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc
    10861 agccgggaac gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta
    10921 tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa
    10981 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
    11041 ccaatccga
//
LOCUS       DAJTUM010000117        10948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTUM010000117 DAJTUM010000000
VERSION     DAJTUM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO471_003435"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO471_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6244573.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO471_003436"
     CDS             complement(1433..1759)
                     /locus_tag="OO471_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6244574.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO471_003437"
     CDS             1894..2235
                     /locus_tag="OO471_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6244575.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO471_003438"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO471_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO471_003439"
     CDS             complement(2833..3543)
                     /locus_tag="OO471_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6244576.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO471_003440"
     CDS             3651..3956
                     /locus_tag="OO471_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6244577.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO471_003441"
     CDS             4155..4475
                     /locus_tag="OO471_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6244578.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO471_003442"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO471_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO471_003443"
     CDS             complement(6299..7288)
                     /locus_tag="OO471_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244579.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLSTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO471_003444"
     CDS             complement(7373..7864)
                     /locus_tag="OO471_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244580.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO471_003445"
     CDS             complement(8185..8685)
                     /locus_tag="OO471_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244581.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO471_003446"
     CDS             complement(8675..9190)
                     /locus_tag="OO471_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244582.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO471_003447"
     CDS             complement(9621..9989)
                     /locus_tag="OO471_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244583.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..>10948)
                     /locus_tag="OO471_003448"
     CDS             complement(10401..>10948)
                     /locus_tag="OO471_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6244584.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2811 a         2784 c         2704 g         2649 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 acagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca
    10861 gaccgagcgc cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg
    10921 tgagccgtgt gtcgtgtgaa ctgatact
//
LOCUS       DAJTUM010000118        10926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTUM010000118 DAJTUM010000000
VERSION     DAJTUM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            154..504
                     /locus_tag="OO471_003449"
     CDS             154..504
                     /locus_tag="OO471_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6244585.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO471_003450"
     CDS             557..952
                     /locus_tag="OO471_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6244586.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO471_003451"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO471_003451"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6244587.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO471_003452"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO471_003452"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6244588.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO471_003453"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO471_003453"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6244589.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO471_003454"
     CDS             complement(5323..6423)
                     /locus_tag="OO471_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6244590.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO471_003455"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO471_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6244591.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO471_003456"
     CDS             complement(7571..8137)
                     /locus_tag="OO471_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6244592.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO471_003457"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO471_003457"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6244593.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OO471_003458"
     CDS             10263..10751
                     /locus_tag="OO471_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6244594.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJTUM010000119        10828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTUM010000119 DAJTUM010000000
VERSION     DAJTUM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            208..1662
                     /gene="amn"
                     /locus_tag="OO471_003459"
     CDS             208..1662
                     /gene="amn"
                     /locus_tag="OO471_003459"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6244595.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2005..2721
                     /locus_tag="OO471_003460"
     CDS             2005..2721
                     /locus_tag="OO471_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6244596.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3177..3252)
                     /locus_tag="OO471_003461"
     tRNA            complement(3177..3252)
                     /locus_tag="OO471_003461"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3217..3219),aa:Asn,seq:gtt)
     gene            complement(3353..4980)
                     /gene="yeeO"
                     /locus_tag="OO471_003462"
                     /pseudo
     CDS             complement(3353..4980)
                     /gene="yeeO"
                     /locus_tag="OO471_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4985..5060
                     /locus_tag="OO471_003463"
     tRNA            4985..5060
                     /locus_tag="OO471_003463"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5018..5020,aa:Asn,seq:gtt)
     gene            complement(5098..6048)
                     /gene="cbl"
                     /locus_tag="OO471_003464"
     CDS             complement(5098..6048)
                     /gene="cbl"
                     /locus_tag="OO471_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6244597.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6150..7067)
                     /gene="nac"
                     /locus_tag="OO471_003465"
     CDS             complement(6150..7067)
                     /gene="nac"
                     /locus_tag="OO471_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6244598.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7395..7470
                     /locus_tag="OO471_003466"
     tRNA            7395..7470
                     /locus_tag="OO471_003466"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7428..7430,aa:Asn,seq:gtt)
     gene            complement(7526..8461)
                     /gene="ldtA"
                     /locus_tag="OO471_003467"
     CDS             complement(7526..8461)
                     /gene="ldtA"
                     /locus_tag="OO471_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6244599.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8523..9602)
                     /gene="cobT"
                     /locus_tag="OO471_003468"
     CDS             complement(8523..9602)
                     /gene="cobT"
                     /locus_tag="OO471_003468"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6244600.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9614..10357)
                     /gene="cobS"
                     /locus_tag="OO471_003469"
     CDS             complement(9614..10357)
                     /gene="cobS"
                     /locus_tag="OO471_003469"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6244601.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10354..>10828)
                     /gene="cobU"
                     /locus_tag="OO471_003470"
     CDS             complement(10354..>10828)
                     /gene="cobU"
                     /locus_tag="OO471_003470"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6244602.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3022 a         2758 c         2336 g         2712 t
ORIGIN      
        1 cacttacttt gccgggaact ggcatagcat cgccatttat ttgctggctg gatgcctgat
       61 ttctgcaatg accgctttat tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga
      121 tcatccgatc ttcgccttat acttttgttt cacattcctg tgacatacta tcggatgtgc
      181 ggtaattgta tggaacagga gacacacatg aataataagg gctccggtct gaccccagct
      241 caggcactgg ataaactcga cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc
      301 attggcaaat atattacaat tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt
      361 tttgtctatc catcactgac cgtaacctgg gacggtagca caacaaatcc ccccaaaacg
      421 cgcgcatttg gtcgttttac tcacgcaggc agctacacca ccacgattac tcgccctact
      481 ctctttcgtt cgtatctgaa tgagcaacta acgttgcttt atcaggatta tggtgcgcat
      541 atctctgtgc aaccctcgca acatgaaatc ccttatcctt atgtcatcga tggctctgaa
      601 ttgacacttg atcgctcaat gagcgctgat ttaactcgct acttcccgac aacagaactg
      661 gcgcaaatcg gcgatgaaac tgcagacggc atttatcatc caacggaatt ctccccgcta
      721 tcgcattttg atgcgcgccg cgtcgatttt tccctcgcac ggttgcgcca ttataccggt
      781 acgccagtag aacattttca accgttcgtc ttgtttacca actacacacg ttatgtggat
      841 gaattcgttc gttggggatg cagccagatc ctcgatcctg atagtcccta cattgccctt
      901 tcttgtgctg gcgggaactg gatcactgcc gaaaccgaag cgccagaaga agccatttcc
      961 gaccttgcat ggaaaaaaca ccagatgcca gcatggcatt taattaccgc tgatggtcag
     1021 ggtattactc tggtgaatat tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat
     1081 ctggcagtgc tacgcccgga tatctggttg atgattggtc actgtggcgg attacgtgaa
     1141 agccaggcca ttggagatta tgtccttgca cacgcttatt tacgcgatga ccacgttctt
     1201 gatgcggttc tgccgcccga tatacctatt ccgagcattg ctgaagtgca acgtgcgctt
     1261 tatgacgcta ccaagttagt cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt
     1321 acaggtactg tggtaaccac agatgacagg aactgggagt tacgttactc agcttctgca
     1381 cttcgtttta acttaagccg ggccgtagca attgatatgg aaagtgcaac cattgccgcg
     1441 caaggatatc gtttccgcgt gccatacggg acactactgt gtgtttcaga taaaccgtta
     1501 catggtgaga ttaagcttcc cggccaggct aaccgttttt atgaaggcgc tatttccgaa
     1561 cacctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat
     1621 tcacgtaaat tacgaacctt taatgagccg ccgttccgat aataagttat gaaccttctt
     1681 atcagaaata gatgtgaagg attaagtaag acatctggca aatagcctgc aataaacgcg
     1741 ggctgcttgc ttccttgggc agatgcgagt tttattatcg tcttaatgat ttccacatat
     1801 taaaagcaag tatgctttca aaacacaatt ataaaaaatc ccgccaacaa cattagtttt
     1861 tataaaatta aatataagat tatggcttta gaatattttt atttctaata gacgagattt
     1921 ttcctgttat gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa
     1981 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
     2041 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
     2101 gctgccaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
     2161 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
     2221 ggcggcggag atgaaacgtt cgtacaggga cgttatgaag gctttggtcc taatggctca
     2281 atgattattg ctgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
     2341 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
     2401 aatacgggtg tgattgtatt taaaggaaca gaccctgacc atatttttga aattttgctt
     2461 gaagctgaag ttgatgttcg tgatgtaact gaagaagaag gtaatattgt tatttatact
     2521 gaacctgctg accttcataa aggaatcgcg gcgctaaaag cagctggaat cactgagttc
     2581 tcaacaacag aattagaaat gattgctcaa tctgaagttg agctttcccc agaagattta
     2641 gaaatctttg aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat
     2701 cataacgtcg caaatctcta attatatttt aaagaaatct gtctttatgg cagatttctt
     2761 taatctcata taattcttat aaaaaatata atatttaact cgtcatattg cttataaaaa
     2821 atccccgtcc ccagagaaat aatatttatt aaaatggcag gtcttctttt tctgattacc
     2881 gaagacaaag tggcaattac gtatagtttc ccgataaaga cgcgacagct acgtcccgca
     2941 taaggcattt ttctctttat ctttgtacgg tacttcatgg aacagaattt ttgaccttgt
     3001 gaatcgtgat atctgttggg gaggggcaat ttgctcactg aagcgtgaga ctcgattaag
     3061 cgcacgaaac acagaaatca aaaaacccgg ccactttttt acaaagtggc cgggtaaaaa
     3121 taatttttat tttttaactg tcctgagtct catagagatg tctcaaaact aaaatttggc
     3181 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
     3241 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
     3301 gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
     3361 acacaactgg ctttttttca cacttttgcg gctcggatcg agggtatttc cacagccaac
     3421 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
     3481 ccatccagac accaaccaca ccccagccaa gcatgattcc ctgcacataa ccgactacaa
     3541 cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca tcacgagcac
     3601 ccttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat aaagcattta
     3661 gctaaatcag aatcacaacg acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca
     3721 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggacagcc
     3781 agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc
     3841 ctgtaatgat cgtagaagca gaaccgagcg cacttccggg taagttgata agagccgcaa
     3901 ttgagaacgc gataaaattt ccggcaataa cgctggtccc catcccggca acgaacattt
     3961 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgctgga ataccaatcc
     4021 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
     4081 ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac
     4141 gagaaatggt taaacccaac ccagccccga ccaggagaaa aggccgtaaa tcaatatgcc
     4201 gctaataata atattaagaa tattcaggct accgttaatc agtagcggta ttttcgtatt
     4261 ccctgcacca cgaagtgccc cgctaccaat aagagtgatg gcagcggctg gataactgag
     4321 tacggtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg catcacccgc
     4381 gacgaaatca ataatttgtt cgccaaaatg atgaattagc gttgccaaca gtacggcaaa
     4441 caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt
     4501 accgagacta aatgctacaa cgacagtagt accaagatcg atagctgcaa aaaaagccat
     4561 aatgaccata ttgaagctgt ccgccaaccc cacgccggcc atcgcatctt ttcccagcca
     4621 actgaccaga aaagtgctca ggacccccat caacaggaca caggcgttct ccatgaagat
     4681 aggaacagca agcggggtta tctcgcgcta gaacaacact ttgtagctct tgcgtttaga
     4741 gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca aagccgacct
     4801 taattgcaga aagtgaaacc acatttcaaa tgatgaggga gaatcagcaa agctgcaaag
     4861 attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt
     4921 gggaaattta aaaatcgggt ttgacaaaat atttttcgcc gttaagatgt gcctcaacag
     4981 cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc
     5041 gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg ctttttgcta
     5101 tatctgataa tcaatttcct cttcactgtt ttccatcacc tggcgcttga tatcctcaac
     5161 tgacagtcct gcattacaaa gttccagaaa gcgccaaaca tagttacgct gaagttgtcc
     5221 tcgcttcagt cccaaccaga cagtattagc atcgaaaaga tgccgcgtat ccaggcggat
     5281 taaattctct tcctcttgtt cgccgctgga ttgttcggca actaatccga tcccaagccc
     5341 aagagcaaca taggttttaa tgacatcaga atcctgtgca cttaatacaa tatctgccgg
     5401 caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc cccgtaattc cctgtcggta
     5461 agtaattaac ggccacttcg ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg
     5521 atgatcgagt ggaacaagca aactatggtg ccaacaaaac catgggaagg cgacgagctg
     5581 cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcgccat tttgcaacaa
     5641 cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc cgaacctccg ggaaaagttc
     5701 gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat
     5761 agtgagaaca ccagacgtat cgttggtaaa caggtctgca agccgacgaa cattactggc
     5821 ttcattcaga atacgttctg caatgaccag taatgctttg cccggttcag tcatccccag
     5881 cagtcgctta cctcgtcgaa caaatatttc gatgccaaat tcatcctcca gttcccgaat
     5941 atgacggcta acgcctgact gtgaggtaaa aagcatattc gcaacctctg tcaggttgta
     6001 atcctgacgt gcagcctcgc ggattatctt tagttgttgg aaattcacgg taaactccgg
     6061 gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg gcataacaaa taataaaaac
     6121 ccgcatctta ttccatcccg atataacgct tagctcacca attgccactg ccttttttcc
     6181 atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc ctgtggtgac
     6241 aagtttgctc tggcgggtaa atttaaggac aaagagagac tcattgaagg agtggtaatg
     6301 cgtgacatcc acccatttac tgcgccacat aacgaacgcg cagccgattc aggtaatact
     6361 gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg caatagactc aatttcacca
     6421 atgacttttg ccgtgagccg ccgtagggaa aaagcctcat caacacgaag tctaacagcg
     6481 ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag cattaacatc aacgttttgc
     6541 cccgggcaat cttgagttcc taccaggaaa agatcttctt tcagcaaagc ctgactggat
     6601 accccagcca caggggaatg ctcataaatc accgccatat cgagttggtg atttatcagt
     6661 ttttcgttaa gcactgcacc actattttca tgaagataga taacgacctc cgggaattca
     6721 gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc tggcgcaaag
     6781 ccaatcgaga cttgccccga taatgactga ccaacgttat gcaccgccag ttgggcctgc
     6841 tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa tttttccggc gtctgttggc
     6901 gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta actcaccttc cagtatggca
     6961 acctgttggc tgagcgctgg ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta
     7021 ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt gcctccggtt
     7081 tttaagaatc ggcccaagtg ccgccattac ttacaaccag tatgcaagat gcctgccagt
     7141 tctattttgg cgttgatgcg caagctaacc aactgtcaaa taagagatta tgatagattc
     7201 gtcattggcc ctttttatca gcagtcgcat tcacctgccc tataaaagga gggtatgcac
     7261 cacgatggtt cattacccaa taagattgaa agctcaccac tttgttgaaa ttggcagcaa
     7321 acaaacaaaa aaaatgcatt tcaccctttg acatcaccat gcactgccat taatatgcgc
     7381 cccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     7441 tcactggttc gagtccagtc agaggagcca aattcaaaaa agcctgcttt cgagcaggct
     7501 ttttactttc taattaccaa cgctcttaaa acatctgtct tgaaccagaa ctaatttgca
     7561 caggcattcc cgatcgatgt tgcaacgcag catttgcgcg atttacatca acttcttgcc
     7621 cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg cacttttcgg tcagactcat
     7681 attctgcacg attgcgcgac agcggctcat gaacttccag ccactttgaa ccatccggtt
     7741 ccgttgtgta tttcactggc tgatcaataa tttgtacacg agtgcctaca ggaacattat
     7801 caaacagata tttgatatcg tcattacgca gacgaataca gccctgactt acacggagcc
     7861 caataccaaa attggcattg gtaccgtgga tggcatacaa cctgccaata taaatcgcgt
     7921 acagccccat ggggttatcg ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt
     7981 tcgcatattc gcgccggatg ttcggcgttg gcgtccaggt tggtgcttct tgtttacgtt
     8041 caacggtagt cacccagtta cgcggggttt ctcgcccagc ctggccgata ccaataggaa
     8101 agacttccac tgtattactg tctggcggat agtaataaag acgcatttca gcgacgttaa
     8161 ccacaatccc tttacgaaca gtggcgggca aaatcagttg ctgcggaatg gtgagttgtg
     8221 agccaggctt cggcaaaaaa acatcagcgc ccggattcgc ttccagcata ttacttaacc
     8281 cttgcccgta ttgtgcggca aaagtctcca gcggctgagt attgtgatca ggaacagtta
     8341 cagtaagcga ctgccccact aaacggctac cctctggagg taatggataa gttaccgcca
     8401 ggctattttg gctggcaaaa agcagagcaa atgagcaaag aatatttaca cgacgcatca
     8461 tgtccctttc ctaagtcgcg aagactatcc gataagtata gcttttatca gacttttcgt
     8521 ttttagctgt tcaaatcaga agtcgtattc cccggtagaa caatattact ggcagcaagt
     8581 tcgcccatgt tgttgtatat cgcacaagca gcttcgatga tggacatcgc cagagctgca
     8641 ccactcccct cacctaaacg catatccata ttgagataag gctccagccc caaatgcgag
     8701 agcgctatac gcgcgccttt ttctgccgac aagtgagaag gaatgagata cggtttgatt
     8761 gcaggagaca tctggcaggc tgcgagcgca gcagcataag aaagaaatcc atccagcaac
     8821 acgggtaaac cgcaggaagc agcacctaac atcactccag ctattccgac caaatcaaat
     8881 ccacccactt ttgccaggac atcaacacca tcctgaggat ttggttgatt caacgtaatc
     8941 gcccgacgca caacatcaat tttattagcc agtttatctg tcggcaggtt tgcgccaatc
     9001 ccaaccactt cttcaggatc ccggccagtg attgtgctga ctattgccgc tgccggtgtc
     9061 gtgtttgcca tccccagttc acctacacca aacagcgtga caccgttttt cgccagctcc
     9121 cgcgtataac atatgacgtc caaaagcaac ttttcagcct gacggcgact cattgccgga
     9181 gctgaagcaa tattgccgct acctcgtgcg acacgcatgt tgataagccc ggggataggc
     9241 tcagcagtat caataccaac atcaattacg tggacgttag cgcccgcttg tgctgccagc
     9301 acacacacgc cggttgttcc acgggtcata ttttcagcct gtatggctgt cacttctttt
     9361 ggagaaatag cgaccccttc ctcccagacg ccgtgatcgg cacacataac cagtaccgct
     9421 tttttgccca catgcggtat gccattcacc cccggcattc ctgccagttg tatggcaagc
     9481 gcctccagct ttcctaggct accaacaggt ttgaataacc cgtcaacatg ccgttgtgca
     9541 cgcgacatag cggcagaatt gatggctggg atcgtattca gtaaatcggc aattatttgc
     9601 atgtcacgtt tctttatagc agtgccagca ggaagaccag ttcaccaagc tctatggctg
     9661 ctcccagcgt atcgcccgtt tgcccgccca gcgtacgttt gagaagctga ccgaggatga
     9721 aaatcgccac catcgtcacc accatagcag ccacaccatg catacccagc aataatacgg
     9781 cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg tcgcccatca attttgccaa
     9841 tgaacacatt gccaagccct tcttcccgcg cataacgatg acgatacatc aataatgcgg
     9901 cagtcccgcg gctgaccgca catgctgccg ccagcgatgc aaggatcggt tcgccacgca
     9961 gagccaactc gcttaacacc agaatctttg ccagtaccac aaaaatcaat gccagaccgc
    10021 cgtgggtgcc taaacggcta tcacgcatga tttccaacat gcgatcgcga ctacgtgcag
    10081 aaaatacgcc atcacaggta tcagcgaggc cgtccagatg gaattccccg gtcatcagcg
    10141 ccagcacaag tacgctaaac agtgccgcca gtggtgcgcc acaccatgcc tgcagcacca
    10201 tgaagaccag cccgctaatc gcgccaagca ataatccaat caaaggaaaa gtaataatac
    10261 cgcgagaata atgttcgaaa tccagtccct gggaccagcg acgcggtaca ggcagacgcg
    10321 taataaatga gagcatcgcc caaaataatt tgctcatttg atttttactc caatacccga
    10381 aaccaccagc catacttcat ttgccgcagc ggccaactgc tgatttaccc gcccggcaat
    10441 atcacgaaaa tgtcgtgcca gacgactctc cggcacaatc cccattccca cttcgttagt
    10501 cactaataca acctttgcgg ggcaacgttg gcaggcagca atcaacgact gaatctcagc
    10561 attaatcgcc tgttccatcg cctgataatc ccattcatca gggtctttat cgccgccata
    10621 atcaaacaac agattagtca ccattgtggt aacgcattcc agcaacacag cctcattcgg
    10681 gttaatgtct gcatgaatta attcatcaag atgttgccag cgctccactg tgcgccagtg
    10741 ctccgggcgg ctttgccgat gatgttctat ccgtgcagcc atctcatcat caaggatttg
    10801 cgaggtagcg atatacagaa cctgtgaa
//
LOCUS       DAJTUM010000120        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTUM010000120 DAJTUM010000000
VERSION     DAJTUM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO471_003471"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO471_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6244603.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO471_003472"
     CDS             214..402
                     /locus_tag="OO471_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6244604.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO471_003473"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO471_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6244605.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO471_003474"
     CDS             597..770
                     /locus_tag="OO471_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6244606.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO471_003475"
     CDS             complement(819..1892)
                     /locus_tag="OO471_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6244607.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO471_003476"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO471_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO471_003477"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO471_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6244608.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO471_003478"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO471_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6244609.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO471_003479"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO471_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6244610.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO471_003480"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO471_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO471_003481"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO471_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6244611.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2684 c         2787 g         2468 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aactgtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattacagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTUM010000121        10293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTUM010000121 DAJTUM010000000
VERSION     DAJTUM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..401
                     /locus_tag="OO471_003482"
                     /pseudo
     CDS             1..401
                     /locus_tag="OO471_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OO471_003483"
     CDS             484..741
                     /locus_tag="OO471_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244612.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO471_003484"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO471_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6244613.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO471_003485"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO471_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6244614.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO471_003486"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO471_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6244615.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO471_003487"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO471_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6244616.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO471_003488"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO471_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6244617.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO471_003489"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO471_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6244618.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO471_003490"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO471_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6244619.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OO471_003491"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OO471_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OO471_003492"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OO471_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OO471_003493"
     CDS             9762..10019
                     /locus_tag="OO471_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR6244620.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2400 a         2735 c         2822 g         2336 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agc
//
LOCUS       DAJTUM010000122        10099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTUM010000122 DAJTUM010000000
VERSION     DAJTUM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            415..546
                     /locus_tag="OO471_003494"
     CDS             415..546
                     /locus_tag="OO471_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6244621.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            806..1801
                     /gene="pgl"
                     /locus_tag="OO471_003495"
     CDS             806..1801
                     /gene="pgl"
                     /locus_tag="OO471_003495"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6244622.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1842..2795)
                     /locus_tag="OO471_003496"
                     /pseudo
     CDS             complement(1842..2795)
                     /locus_tag="OO471_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2979..4031
                     /locus_tag="OO471_003497"
     CDS             2979..4031
                     /locus_tag="OO471_003497"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6244623.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4107..5540
                     /locus_tag="OO471_003498"
     CDS             4107..5540
                     /locus_tag="OO471_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6244624.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5723..7903
                     /locus_tag="OO471_003499"
     CDS             5723..7903
                     /locus_tag="OO471_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6244625.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8044..9327)
                     /locus_tag="OO471_003500"
     CDS             complement(8044..9327)
                     /locus_tag="OO471_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6244626.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9462..>10099)
                     /locus_tag="OO471_003501"
     CDS             complement(9462..>10099)
                     /locus_tag="OO471_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6244627.1"
                     /translation="TLSDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIY
                     QAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLH
                     VDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPP
                     TFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2485 a         2341 c         2531 g         2742 t
ORIGIN      
        1 gccataaggg aatgagtgtt atatcatgct ttctacccta gtaagtatat ttgctttttt
       61 cgtatatttg gttcatcttt ttttgatgtt ttacatattc acaatgcaga ggatttatac
      121 cagaggacat tagtcttgag taagatacta caacatctcc aactatttta gccagcctga
      181 ctacttgact tggtaatatt tattgctaat aatcatgtat aaaataagct tatatacata
      241 gtaacttcag caaaggattt agtaatgatc aaaatcatat ctcaatgtac tgtttttttc
      301 tttaaaaaaa cagtacattc tattttataa tgactgtgta ttcccatgga aggagatcac
      361 ccaattctcc cccccgactt ttgacgcagt gtcttgttaa cagggggatt gaccatgctt
      421 attggctatg tacgcgtatc aacaaatgac cagaataccg atctacaacg taatgcgttt
      481 atttgtgccg gaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg
      541 gcatagtgtt tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga
      601 atagtcgcga aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa
      661 tatgccattc gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt
      721 atcgtgtttt gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg
      781 gatttatcat ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag
      841 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
      901 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
      961 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact
     1021 gacctttgcc gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca
     1081 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
     1141 agatggcctg ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc
     1201 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
     1261 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
     1321 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg
     1381 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
     1441 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
     1501 tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct
     1561 gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc
     1621 aacggaaacc cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc
     1681 tgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca
     1741 tgaaaaaggc cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
     1801 accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac
     1861 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
     1921 ttaatcagca aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga
     1981 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa
     2041 tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact
     2101 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
     2161 tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt
     2221 ttgtaaccca ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc
     2281 cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
     2341 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
     2401 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
     2461 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca
     2521 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
     2581 tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
     2641 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca
     2701 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
     2761 acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac
     2821 gattttgggg ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa
     2881 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat
     2941 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
     3001 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
     3061 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
     3121 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
     3181 atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg
     3241 ataccacgcc taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg
     3301 gtttgattgc agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta
     3361 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
     3421 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
     3481 gaacgaaaac tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
     3541 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
     3601 aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
     3661 aatctatccg gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta
     3721 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
     3781 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
     3841 gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata
     3901 tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg
     3961 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
     4021 atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
     4081 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
     4141 tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg
     4201 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc
     4261 cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat
     4321 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
     4381 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta
     4441 ttgcctatct gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg
     4501 tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
     4561 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
     4621 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
     4681 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
     4741 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
     4801 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     4861 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     4921 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     4981 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     5041 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     5101 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     5161 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     5221 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     5281 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     5341 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
     5401 cggtcatctt tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac
     5461 tgacgatatt aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
     5521 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
     5581 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct
     5641 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
     5701 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
     5761 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
     5821 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
     5881 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
     5941 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
     6001 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
     6061 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
     6121 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
     6181 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
     6241 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
     6301 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
     6361 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
     6421 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
     6481 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta
     6541 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
     6601 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     6661 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     6721 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     6781 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     6841 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     6901 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     6961 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     7021 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     7081 agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     7141 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     7201 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     7261 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     7321 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     7381 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     7441 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     7501 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     7561 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     7621 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     7681 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     7741 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     7801 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     7861 ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg
     7921 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     7981 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     8041 ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     8101 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     8161 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     8221 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     8281 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     8341 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     8401 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     8461 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     8521 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     8581 accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc
     8641 cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag
     8701 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     8761 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     8821 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     8881 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     8941 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     9001 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     9061 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     9121 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     9181 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     9241 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     9301 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     9361 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     9421 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     9481 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     9541 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     9601 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     9661 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     9721 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     9781 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     9841 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     9901 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     9961 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
    10021 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
    10081 aatgcatcgc tcagtgttg
//
LOCUS       DAJTUM010000123        10051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTUM010000123 DAJTUM010000000
VERSION     DAJTUM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            745..2253
                     /gene="ltrA"
                     /locus_tag="OO471_003502"
     CDS             745..2253
                     /gene="ltrA"
                     /locus_tag="OO471_003502"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6244628.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2343..2816
                     /locus_tag="OO471_003503"
                     /pseudo
     CDS             2343..2816
                     /locus_tag="OO471_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            2981..7099
                     /gene="pic"
                     /locus_tag="OO471_003504"
     CDS             2981..7099
                     /gene="pic"
                     /locus_tag="OO471_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6244629.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(7250..7396)
                     /locus_tag="OO471_003505"
                     /pseudo
     CDS             complement(7250..7396)
                     /locus_tag="OO471_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7427..8998)
                     /locus_tag="OO471_003506"
     CDS             complement(7427..8998)
                     /locus_tag="OO471_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6244630.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
     gene            complement(9018..9365)
                     /gene="tnpB"
                     /locus_tag="OO471_003507"
     CDS             complement(9018..9365)
                     /gene="tnpB"
                     /locus_tag="OO471_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6244631.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            complement(9365..10042)
                     /locus_tag="OO471_003508"
     CDS             complement(9365..10042)
                     /locus_tag="OO471_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6244632.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
BASE COUNT         2499 a         2470 c         2769 g         2313 t
ORIGIN      
        1 gcaggcgacc gcgtaaaccg tcagttcgtg gcagaacgac ctgaccagct gtgggtggct
       61 gattttactt acggtgtgcc cggagttcag ggcgggcatg gatgcttaaa tgaaccgcga
      121 gtctgtctgg aatattgaac cggtaactca cgatgagaaa cccaacaatc ccaccgggtg
      181 tgacggtgga gaacctgagc ggcagtgacc tgcggcatgc ccgcagggtg atgtaacccg
      241 ctgacaacgg ggattgaggc gagatcacta agccgagatg atcctcaagg ttaagtactg
      301 aaaggctgaa gaacatgaac ccgttaatcc gcctctgtgg gttgaaaacg tcaccacggc
      361 ctacgtgatc tgacaggccg tgcaggagga actggcagtg atacgtaagc actgccggtc
      421 gaaggtgttt tgacatgtat gcgaaacacc ggggcagcag cgtctatcac acttgcgttg
      481 ctgacttctg ccaacttgcg gcaagcaagg ataaagagtg cgacgggcag cctcctcagt
      541 atgcctgagt ccaggcaggt aaaccgggga aggtcagcga cggatgttaa gggggcatgg
      601 ctccgatgac gcgctggctg gcggagcttc cgtagtagtc cgcgatgggg aaagcccatt
      661 acatggcgaa gggaagcagt ttgaatgtgt ttgcgacgtg aattaactga cctaacgagg
      721 tgaagacctt tgataatcag cgaaatgcaa cgcaagcttg ccacatgggc agccaccgat
      781 ccgtccctac ggattcaacg gctgctgcgt ctgataacac aaccagaatg gctggctgaa
      841 gcggcgcgga tcacgctttc atcaaagggg gcccataccc ccggcgttga tggcgtgaac
      901 aaaacaatgc tacaggccag actggctgtt gagctgcaaa tcctcaggga tgaattactc
      961 tcaggccact accagccctt gcccgccaga cgggtttaca tccctaaaag caacggcaaa
     1021 ctgcgaccac tgggtatccc cgcgttgcgg gatcgtattg ttcagcgggc catgctgatg
     1081 gcgatggagc cgatatggga gagtgatttt catacgctct catatggctt ccggcctgag
     1141 cgcagtgtcc accacgcgat ccgcacggtg aaattacagc tcacagactg tggtgaaacc
     1201 cggggacgct gggtgattga aggcgacctg tccagttact tcgacaccgt acatcatcga
     1261 ctgctgatga aggctgtacg ccgcaggatc agtgacgcac gtttcatgac tctgctgtgg
     1321 aaaaccatca aggcgggaca tatcgatgtc ggtctctttc gggcggccag tgaaggtgtg
     1381 ccacagggcg gtgttatatc gccgctatta tcgaacatca tgctgaatga gttcgatcaa
     1441 tacctgcatg agcgctacct gagcgggaaa gccagaaaag atcggtggta ctggaataac
     1501 agtatccaac ggggccgaag tacggcggtc agagaaaact ggcagtggaa acccgcggtg
     1561 gcgtactgcc gctatgccga tgattttgtc ctcatcgtca aaggcaccaa agcacaggcg
     1621 gaagccatca gggaggagtg tcggggtgtg ctcgaaggca gtctgaaact caggctgaac
     1681 atggataaga ctaaaatcac ccatgttaat gacggcttta tctttctggg gcacaggatc
     1741 attcgcaaac gcagtcgtta tggcgagatg cgagtggtct caacgatccc gcaggagaaa
     1801 gccagaaact tcgccgcatc gctgacagca ctgttatcag gcaactacag tgaaagcaaa
     1861 gtcgatatgg ctgaacaact caaccgaaaa ctgaaaggct gggccatgtt ctatcagttc
     1921 gttgatttta aggccaaagt cttcagttat atcgaccgtg tcgtgttctg gaagctggct
     1981 cactggctgg cccgcaaata ccgtacaggt atcgcttccc tgatgaggtg gtggtgtaaa
     2041 tcaccgaaac cgggtcagag caaaacgtgg gttttatttg gtaaaaccaa tcacggcaag
     2101 ctcagcggcg aaatactgta ccggttggtg gggcaaggca agaagctgtt ccgctggcgg
     2161 ctacccgaag gtaatcccta tctgaggacg gagaccagaa acacgtatac atcgcgcttt
     2221 acagaagtgg caatggcgtt cgccagcatt taaatggaga gccggatgcg ctgaaaggtg
     2281 cacgtccggt tcggggagga gaggcaggga aatagtccga ctacgccctg cctcttactc
     2341 tactcagcac atggcagggc ttcgtctatg tggcgttcat cattgatgtg tttgccggat
     2401 acatcgtgtg gtgacgagtc tcatcgtcta tggaaacgac attcgtgctg gatgcactgg
     2461 agcaggcgtt gtgggcccgt cgtccgtctg gcaccatcca tcacagcgat aaaggctctc
     2521 agtatgtgtc actggcctat aaggcgcgac taaaagaagc aaaactgctg gcatcaacag
     2581 ggagtactgg tgactcgtat gacaacgcga tggcagagat catcaagggc ctttacaaag
     2641 cggaggtaat acaccgtaag agctggaaaa accggacaga agtggagctg gccacactca
     2701 cgtgggtgga ctggtataac aatcgacgat tgctggaaag gctgggccat atcccaccgg
     2761 cagaagcaga aaaagcttat tatgcttcca tcggaaatga tgatctggca gcctgagttc
     2821 acagataaaa cactctccag gaaacccggg gcggttcagt tcacaaaaac acattaatgc
     2881 agtaactata ttttcctttc tggtgataac gtcgggttat cattagcttc ttcagctatt
     2941 ttacttttat atcccttgta aacatcatgg agaatccata gtgaataaag tttattctct
     3001 taaatattgc cccgtcaccg gggggcttat tgctgtctct gaacttgccc gcagggtaat
     3061 aaaaaagaca tgccgaagat taacgcatat tcttctggct ggcattccag caatctgtct
     3121 gtgttactct cagatatccc aggcgggtat tgtccgttcc gatattgcct atcagattta
     3181 tcgtgatttc gccgaaaaca aagggctttt tgtacctggt gccaatgata ttccggtata
     3241 tgataaggac ggaaaacttg tgggaagact gggtaaagcc ccaatggccg atttcagcag
     3301 tgtgagctca aatggcgttg ctacgcttgt atcgcctcag tatatcgtca gcgtaaagca
     3361 taacggagga tatcggagtg tgagctttgg taatgggaaa aatacatatt cccttgttga
     3421 ccgtaataac cacccttcta ttgacttcca tgctccacgt ctgaataaac tggttacaga
     3481 agttattccc tcagcggtaa catcagaagg aaccaaagcc aatgcttata aatacactga
     3541 acgttacacc gctttttatc gggtgggtag tggtacgcag tacactaagg acaaggacgg
     3601 aaatttagtt aaggttgccg gtggatatgc ttttaaaaca ggaggaacca caggagttcc
     3661 tctgatatct gatgcaacaa tagtctctaa tcccgggcaa acttataatc ctgtaaacgg
     3721 ccctttacct gactatggag cccctgggga tagtggttct cctttgtttg cttatgataa
     3781 acaacaaaaa aaatgggtta ttgttgctgt attaagagca tatgcaggta ttaatggtgc
     3841 tacgaactgg tggaatgtca taccaacaga ttatctgaac caggttatgc aggacgattt
     3901 cgatgccccc gtagactttg tttccggact gggccccctg aactggacat acgacaaaac
     3961 atcaggcaca ggtaccctga gccagggcag taaaaactgg accatgcacg ggcagaaaga
     4021 caatgacctc aatgccggta aaaatctggt attcagcggg cagaatggtg caattatcct
     4081 gaaagacagt gtgactcagg gtgccggtta tctcgaattt aaagacagtt acaccgtatc
     4141 tgctgaatcc ggaaaaacat ggacgggtgc cggcattatt actgacaagg ggacgaatgt
     4201 aacctggaag gtcaacggcg ttgccggtga caacttgcat aagctggggg aaggaaccct
     4261 gaccataaac ggaacaggtg taaacccggg aggactgaaa acgggagacg gtatcgttgt
     4321 acttaaccag caggcagaca ctgcaggtaa tatccaggcc ttcagttcag tgaacctcgc
     4381 cagcggacgt ccgaccgtgg tgctcgggga tgcccgtcag gtcaatccgg ataacatttc
     4441 atggggatac cggggaggta agcttgacct taatggtaat gccgttacct tcacccgact
     4501 gcaggctgct gattacgggg cggtgattac aaataatgca cagcaaaaat cccagctttt
     4561 actggatctt aaggctcagg atacaaatgt cagtgaaccg acgattggaa atatatcccc
     4621 ctttggtggt accggcactc caggaaacct gtacagcatg atactcaaca gccagacccg
     4681 cttctatatt ctgaaatctg ccagctatgg taacactctg tgggggaaca gcctgaatga
     4741 tccggctcag tgggagtttg ttggcatgaa caaaaacaaa gcagttcaga cagtaaaaga
     4801 taggatcctg gccgggcggg caaaacaacc cgttatcttt catggtcagc tgaccgggaa
     4861 tatggatgtc gccattccac aggtgccggg gggaagaaag gtaatctttg atggtagcgt
     4921 gaacctgccg gaaggtaccc tgagtcagga cagtggcacc ctgatattcc agggacatcc
     4981 ggttatccat gcctccatca gtggcagtgc accggtcagc ctgaaccaga aagactggga
     5041 aaaccgtcag tttacaatga aaacactgtc gctgaaagac gctgacttcc atctttcacg
     5101 taacgcctcg ctgaacagtg acattaagtc ggataacagc catatcacac tgggaagtga
     5161 cagggcattt gtggataaaa atgacggaac aggaaattat gtcattccgg aggaaggtac
     5221 ctctgtcccg gacaccgtga atgacaggag ccagtatgaa gggaatatta cgctgaacca
     5281 taactcagcc ctggatatcg gcagcaggtt caccgggggg attgacgctt atgacagtgc
     5341 cgtcagcatc acctctccgg acgtcctgtt gacagccccg ggtgcttttg ccggcagttc
     5401 actgacagtg catgatggcg gtcatcttac agcactgaac ggtcttttca gcgacgggca
     5461 tattcaggcc ggtaagaacg gcaaaatcac cctgagcggt acaccggtta aagatacggc
     5521 taatcagtat gcccctgctg tatatctgac ggacggatat gacctgaccg gcgataacgc
     5581 agcactggaa attacccgtg gagcacatgc ttccggtgat attcatgcct ctgcggcatc
     5641 aacagttacc atcgggtctg acacgccggc agaactggct tctgcggaaa cggctgcatc
     5701 ggcgtttgcc ggcagtcttc ttgagggcta taacgcagca ttcaatggtg ccataaccgg
     5761 tggcagggct gatgtcagta tgcataatgc actgtggact ctgggtgggg actctgccat
     5821 ccacagtctt accgtcagaa acagccgtat tagttctgaa ggagaccgta cattccgtac
     5881 cctgacggtg aataaactgg atgcaacagg cagtgatttt gttttgcgta cggacctgaa
     5941 aaatgccgat aaaattaatg tgactgaaaa agccactggt tcagataaca gcctgaacgt
     6001 cagctttatg aataatcctg ctcagggaca ggccctgaat attcctctgg tcacggcacc
     6061 ggcgggaact tcagcagaga tgtttaaggc cggcacccgg gtgacaggtt tcagtcgggt
     6121 gaccccaacc ctgcatgttg ataccagtgg tggcaatacg aagtggatac tggatggttt
     6181 taaagcggag gctgataaag ccgctgccgc gaaggctgac agtttcatga atgccgggta
     6241 taaaaacttc atgacggaag ttaacaatct gaacaaacgt atgggtgacc tgcgtgacac
     6301 aaacggtgat gccggtgcct gggcgcgcat catgagtggt gccggttctg cagacggtgg
     6361 ttacagtgat aattacaccc atgttcaggt cggctttgac aaaaaacatg aactggacgg
     6421 tgtggacctg tttaccggtg tcacgatgac ctataccgac agcagtgcag acagccatgc
     6481 attcagcgga aagacgaaat cggtgggggg cggtctgtat gcttcagcat tgtttgagtc
     6541 cggtgcctat atcgatttga ttggtaaata tattcaccat gacaatgatt acacaggtaa
     6601 ctttgctagc ctgggaacga aacactacaa cacccattcc tggtatgccg gtgctgaaac
     6661 gggttaccgc tatcacctga cagaggacac gttcattgag ccgcaggctg aactggttta
     6721 cggcgccgtg tccgggaaaa cattccgctg gaaagacggt gatatggacc tgagcatgaa
     6781 gaacagggac ttcagtccgc tggttggaag aacaggggtt gaactgggca agaccttcag
     6841 tggtaaggac tggagtgtga cggcccgtgc cggaaccagc tggcagtttg acctgctgaa
     6901 taatggagag accgtactgc gtgatgcgtc cggggagaaa cggataaaag gagagaagga
     6961 cagccggatg ctgtttaatg ttggtatgaa tgcgcagata aaggacaata tgcgctttgg
     7021 tctggagttt gagaagtcag cctttggtaa atataacgtg gataatgcgg taaacgcgaa
     7081 tttccggtat atgttctgat gtgggggtaa agggctgccg gaaggcagcc tttctcagag
     7141 actcttaaaa tataaaactg aaggtcttcg gtaaggggga gtgtattaga cgtcaactag
     7201 ttttgccgac tggcgcgagc ctgtttaaga ttctgtgtaa atgcctttct cagaagtgac
     7261 cgtccaggcg gtcaccgaac tcgataataa agcggctcat tgccattcgc cagtccttca
     7321 acggcatcgt ccatttctgg gacgcagact ggattgccag ccacaccact tttttcaccg
     7381 agtcgtctgt cgggaagtaa gcggctcgtc agaaccgtat tgatatttac tgagagctca
     7441 gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggccagtcc tggatatgtt
     7501 caatgacgta acgcagccac ttttctggct ccacattgtt cagacggcat gtgccgatca
     7561 gcgagtacaa caccgccgca tgttcaccac cgctgtcgga acccgcgaac agccagtttt
     7621 tccggcctac ggccactccc cgtaaggcgt tctctgcgat gttgttgtcg atttccaccc
     7681 agccattact gcagtacacg ttcagggcct cccactgttt cagcaggtat gcgaacgctt
     7741 ttgccgtatc cgagtgacgc gacagtgttt tcatctgagt ctgtatccag tcatacagtg
     7801 actgcatcag tggcgcagct ctggcttttc ttgctgccag acgctgttct gctgaacatc
     7861 cccggacttc tgcctcgatg gcatacagtt caccgatacg ctgcagggct tccgtggtga
     7921 tatcggttgg cgctcttgca tgcacatcgt ggatttttct ccgggcatgg gccatacacg
     7981 cggcttccgt tattctgccg gattcgtata acgcccggta gccaccgtaa gcatcggcct
     8041 gaagcactcc gctgtaaccg gccaggtgat tttgtggatg gatgcctttc cggtccgggc
     8101 tgtacgcgaa ccagaccgcc gcaggcatct gtgaaccggc gttacggtca tcacggacgt
     8161 agacccacag ccgggctgtc cgggttttac cgctgcccgg ctcctggacc gggacgggga
     8221 tatcatcggc atggacttta ccgggcatca gcacatactg gcgcaggacg tcatacagtg
     8281 gctccagcag ttcagcaaca gcacctgtcc agcgccccag tgtggcacgg ctcagctcca
     8341 cgccctgacg acggtatatt tctgactggc ggtataacgg cagatggtct gcatatttcc
     8401 cggtgacaac atgggcaaga agccccgctc cggcataact gcgtgcaatg ggttttgaag
     8461 gtactggtgc ctgcacgata tggtcgcacc ggcaacaggc cagtttcgga cgttgtgttt
     8521 cgataacctt aaaggcgctg ctgataagct ccagttgctc tgacacatca catcccagag
     8581 aactgagttc accaccacag gcaggacagc attcctcttc cggccggatg acccgggttt
     8641 cacggggaag tgaggccggt aacagtttac gggctgaaga ctggcgcagg gcggatggca
     8701 gtaccgggtc atattgctca cccagcgttt ccgccatttc ttcctgaagt gcgctgattc
     8761 gctcctgtgc ttcctgtatc tgtcgttcgg tttttgcgcg aagtttttct gagcttttac
     8821 cgaactgcat gcgctgcagt tttgcaacca gcgccttcag ccggttgatt tcggaagcat
     8881 aagccgccac ccgcagtgag agcaggcggt tatattcggc catctggcgg atggtgtcct
     8941 gttgcgtctg caacagtgcc cgcaggcggg cattctcatg agcaagtgag gtgtccatat
     9001 cctcacttta caacgggtta tatgcggatt ccagcgcgtt ccgttcgttt cgggtgcttc
     9061 cagttgatac cttcaagaag catggataac tgagccggag taaggtgcac cttgccgtca
     9121 cgggtgactg gccagacgaa gcggccccgc tccaggcgtt tggtgaagag gcacagtccg
     9181 tcactgtcag cccacaacac ttttatctgg tcaccccggc gtccgcggaa gatgaacagg
     9241 tgtccggaga acgggtcatc cttcaggacg ttctgaactt ttgaggccag gccgttaaag
     9301 ccatttcgca tatcggtgat accggcaacc agccagatac gcgaacctgc agggagagat
     9361 atcatcagtg gctgctccct tttatttcgc ggataagtgt ctgtaataac gccggcgtca
     9421 gtttaccttt aagcctgaga gttccggccg gcagaaccag ctcacaacac agactgtcgg
     9481 acggtgtatt tatctgctct ggttcctgtg ccggagccgg gattttatta tccggctccg
     9541 gcgttaacgt cacgggaagc agtgccggca tattttttcc ggaaggcagc aggccacctt
     9601 tccggtattg atggcgccag ttgaagagca ggttatcgtt gattccgttt tcccgggcga
     9661 tctgcgccac acaggctccg ggctgcagtg actgctccac taaggcgatt ttaaactcat
     9721 aagggaagtt gggccgccgg ggacgttttt ttaccacggg ggcttcggat ataacggtgc
     9781 tttcaggacg tacgactggt accgtggaaa attgtccgta aaggcaggca tcaagttcct
     9841 gctccgacat gcctgagggc aaaggccacg aaaggccagc tctccgaaag cgcacaaaca
     9901 tactacaaac tgttgatttt ggtacaccca ggcgacgccc ggccacaacc cggggtaaat
     9961 gttcttcaaa gtgaagacgt aaagcttcag tgatccaggt ccggtgtttc atacgatagt
    10021 gtccattaaa aatgatggac attatttttg t
//
LOCUS       DAJTUM010000124         9938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTUM010000124 DAJTUM010000000
VERSION     DAJTUM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO471_003509"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO471_003509"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6244633.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO471_003510"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO471_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6244634.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO471_003511"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO471_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6244635.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO471_003512"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO471_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6244636.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO471_003513"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO471_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6244637.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO471_003514"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO471_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6244638.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO471_003515"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO471_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6244639.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO471_003516"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO471_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6244640.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9938)
                     /locus_tag="OO471_003517"
     CDS             complement(8990..>9938)
                     /locus_tag="OO471_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6244641.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2586 c         2296 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccgag
//
LOCUS       DAJTUM010000125         9929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTUM010000125 DAJTUM010000000
VERSION     DAJTUM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..336
                     /locus_tag="OO471_003518"
     CDS             <1..336
                     /locus_tag="OO471_003518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244642.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            506..1639
                     /locus_tag="OO471_003519"
     CDS             506..1639
                     /locus_tag="OO471_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244643.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2318..2536
                     /locus_tag="OO471_003520"
     CDS             2318..2536
                     /locus_tag="OO471_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6244644.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /locus_tag="OO471_003521"
     CDS             2754..2996
                     /locus_tag="OO471_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244645.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO471_003522"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO471_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6244646.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO471_003523"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO471_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6244647.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO471_003524"
     CDS             complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO471_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6244648.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO471_003525"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO471_003525"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6244649.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="OO471_003526"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="OO471_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6244650.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2395 c         2544 g         2500 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaatc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttcgtga
//
LOCUS       DAJTUM010000126         9737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTUM010000126 DAJTUM010000000
VERSION     DAJTUM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            359..1477
                     /locus_tag="OO471_003527"
     CDS             359..1477
                     /locus_tag="OO471_003527"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6244651.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1543..1791
                     /locus_tag="OO471_003528"
     CDS             1543..1791
                     /locus_tag="OO471_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6244652.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1856..2224
                     /locus_tag="OO471_003529"
     CDS             1856..2224
                     /locus_tag="OO471_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6244653.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2318..2971
                     /gene="nfsB"
                     /locus_tag="OO471_003530"
     CDS             2318..2971
                     /gene="nfsB"
                     /locus_tag="OO471_003530"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6244654.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3079..4326
                     /gene="ybdG"
                     /locus_tag="OO471_003531"
     CDS             3079..4326
                     /gene="ybdG"
                     /locus_tag="OO471_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6244655.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4394..5770)
                     /gene="pheP"
                     /locus_tag="OO471_003532"
     CDS             complement(4394..5770)
                     /gene="pheP"
                     /locus_tag="OO471_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6244656.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5871..9014)
                     /gene="cusA"
                     /locus_tag="OO471_003533"
     CDS             complement(5871..9014)
                     /gene="cusA"
                     /locus_tag="OO471_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6244657.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9026..9544)
                     /gene="cusB"
                     /locus_tag="OO471_003534"
                     /pseudo
     CDS             complement(9026..9544)
                     /gene="cusB"
                     /locus_tag="OO471_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2381 a         2702 c         2362 g         2292 t
ORIGIN      
        1 ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca cccgcagtta
       61 tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt ttcggacttc
      121 tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc tcaatcgtca
      181 aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag tagccaaagt
      241 gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga acttccgcat
      301 ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag agaaacctat
      361 gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac tggaaatgca
      421 ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga ttgacgcggt
      481 taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta tgctggagct
      541 ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag cgatgcagaa
      601 agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg gcacgccccc
      661 gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac gaacgctgga
      721 aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc atgttggctg
      781 tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg tgccgcactt
      841 tatggccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt ttgccccctc
      901 acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg tcagtaactg
      961 gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg acagcattaa
     1021 agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg ttcgggtgat
     1081 ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc aggccaccgc
     1141 ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc tgctgtataa
     1201 attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg atccgcacac
     1261 tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa tcgccccttc
     1321 agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg tcgtcagcgg
     1381 tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc tgattgggct
     1441 ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc ttacagaaga
     1501 atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca ccctttagaa
     1561 accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct gctgctgccc
     1621 gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca tctgatggat
     1681 cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg cgctattgag
     1741 tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta aacgtcgatt
     1801 atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa cgtttatgga
     1861 taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct ttgtcgagct
     1921 ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga tttttatgct
     1981 gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac aaaaatccct
     2041 gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata aaaagcactg
     2101 gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga
     2161 ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag tgcgtccagg
     2221 ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga accttgtaat
     2281 ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt ctgtcgcctt
     2341 aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg aacaggccga
     2401 gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc cgtggcattt
     2461 tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta
     2521 tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac
     2581 cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg atggccgctt
     2641 tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg cctatatgca
     2701 ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg
     2761 taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg aaggttttga
     2821 cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca gtctggtggt
     2881 tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga aatctcgtct
     2941 gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc tgcccggcta
     3001 tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca taggctgctg
     3061 ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt agcgggtatt
     3121 gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct gaccgccgtc
     3181 gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc
     3241 agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg tttagctttt
     3301 accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa aggcaccgaa
     3361 gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca
     3421 gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc ggcatctcag
     3481 ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt
     3541 ttgatgatct cgctgctgat tggtcagtcg ccagcgattc taatcagcgg tcttggtgca
     3601 atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt ggcaggtatt
     3661 cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc gaaatacggc
     3721 gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa ctgggacaat
     3781 accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa ctggagcggg
     3841 atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt caccagtatt
     3901 cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt aaagccttat
     3961 ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc tacggagtcg
     4021 gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct gaacgaatat
     4081 ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca actggctcca
     4141 ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt gtggctggaa
     4201 tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga agagtttggt
     4261 ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg tgcatttaag
     4321 cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg
     4381 tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc
     4441 acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc agcagcaaaa
     4501 tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc gccttaaact
     4561 gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc aggcagatca
     4621 taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca aacgcttttt
     4681 gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa agcatcagcg
     4741 agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca ttaccctgca
     4801 cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac accgacagcg
     4861 atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg agattatgga
     4921 aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag agtgccagta
     4981 aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga tttaccgctt
     5041 ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc agctccagac
     5101 cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag ccggtggcga
     5161 agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg taaccagaaa
     5221 acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta atcagcgcaa
     5281 accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg ttgatgataa
     5341 taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac tgcatataga
     5401 tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag tagttccagc
     5461 cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca aatgaaccgg
     5521 ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc
     5581 cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc gggccaatgc
     5641 caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata tgacggttat
     5701 gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat acggttgacg
     5761 cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt taacatttag
     5821 tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct ttaattccgt
     5881 acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa cagcgacagc
     5941 aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat cacctctgaa
     6001 ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc caccgtcatc
     6061 gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag cttctgctcg
     6121 ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg taaatacatc
     6181 agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc ggtacccgtc
     6241 gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac cagcgcaaac
     6301 ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag atacaacagc
     6361 acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt ggcgcgctcc
     6421 agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg cactttttca
     6481 gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg ggcatcgata
     6541 taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc ggtagagact
     6601 ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag gatcggcaac
     6661 tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt aattggataa
     6721 cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga agtcacaaac
     6781 aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt aatctcaacg
     6841 ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc tggcaccgtt
     6901 cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac agtgccggaa
     6961 actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg gttacgaatt
     7021 ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag ttcctcaatg
     7081 attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg gatggtcgtt
     7141 tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt tttgccaaat
     7201 acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag catactcgcc
     7261 gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc gccttcattg
     7321 atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt cagcaccgaa
     7381 agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa cagcggatga
     7441 taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat tttgccacgg
     7501 atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc acccgccatc
     7561 gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc ttccagggtg
     7621 aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc cggccccact
     7681 tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt ggcgtcagga
     7741 tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac gatagcggca
     7801 tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt ggcattcagc
     7861 ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga aataatcgcc
     7921 accagcgccg agcgcacatg ccagagaaac ggcgcgcaga ctaccgccac cacaataaac
     7981 tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg gctgcggtcg
     8041 tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc cagtttgtcc
     8101 ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac caccccgccc
     8161 gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg gccaatctgg
     8221 accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt taaaacgatg
     8281 tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata ttctgcttcc
     8341 gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc gctttttact
     8401 tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac ctgatactct
     8461 ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt cagctcatat
     8521 ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc gctgcgatcc
     8581 accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag ctcggcgctg
     8641 actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg cgaacgcgcc
     8701 cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc aaactgcgag
     8761 aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag cggataagtc
     8821 acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat aatcacctgc
     8881 acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca ggtgccccag
     8941 atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga gcgacgaata
     9001 atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca gagcgcatcc
     9061 gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg ccggaaacca
     9121 ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat gcctggaaaa
     9181 cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc cgctgttcgt
     9241 tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg gcggtgttga
     9301 gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg acttccagac
     9361 gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat ttgcggatgg
     9421 tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca tctttcacca
     9481 gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata ccctgaattc
     9541 gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga tttgtgattt
     9601 tagtttggta cgcaatttgg tacacaacac tattttcact tcagcgggta gtcatcaaac
     9661 tcaccataac gagcatcgtt gatgatgtat gtgatcacca caaatatcac atctggacta
     9721 gttccgtctt catgcgc
//
LOCUS       DAJTUM010000127         9683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTUM010000127 DAJTUM010000000
VERSION     DAJTUM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(26..214)
                     /locus_tag="OO471_003535"
     CDS             complement(26..214)
                     /locus_tag="OO471_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6244658.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO471_003536"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO471_003536"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6244659.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO471_003537"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO471_003537"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6244660.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO471_003538"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO471_003538"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6244661.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO471_003539"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO471_003539"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6244662.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO471_003540"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO471_003540"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6244663.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO471_003541"
     CDS             5109..6242
                     /locus_tag="OO471_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6244664.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO471_003542"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO471_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6244665.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO471_003543"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO471_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6244666.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO471_003544"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO471_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6244667.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO471_003545"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO471_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6244668.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO471_003546"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO471_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6244669.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="OO471_003547"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="OO471_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6244670.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2481 c         2466 g         2271 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tct
//
LOCUS       DAJTUM010000128         9616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTUM010000128 DAJTUM010000000
VERSION     DAJTUM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="OO471_003548"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="OO471_003548"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6244671.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO471_003549"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO471_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6244672.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO471_003550"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO471_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6244673.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="OO471_003551"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="OO471_003551"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6244674.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="OO471_003552"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="OO471_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="OO471_003553"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="OO471_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6244675.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="OO471_003554"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="OO471_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6244676.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7297..7566)
                     /locus_tag="OO471_003555"
     CDS             complement(7297..7566)
                     /locus_tag="OO471_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6244677.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7623..8291
                     /locus_tag="OO471_003556"
     CDS             7623..8291
                     /locus_tag="OO471_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6244678.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8607..>9616
                     /locus_tag="OO471_003557"
     CDS             8607..>9616
                     /locus_tag="OO471_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6244679.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSTSAELRQQSFAVAAD
                     AT"
BASE COUNT         2749 a         2189 c         1967 g         2711 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actttggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
     7261 cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg
     7321 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt
     7381 ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg
     7441 aagtggcgca atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca
     7501 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
     7561 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
     7621 atatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
     7681 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
     7741 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
     7801 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
     7861 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
     7921 aacataaaat tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg
     7981 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
     8041 gtgtaatacg caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa
     8101 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
     8161 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     8221 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     8281 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta
     8341 cgatttgggg ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat
     8401 gaacgtacga tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat
     8461 ctaagaaaat agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac
     8521 aataaatttc aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta
     8581 tcaggatatc cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg
     8641 gaaacgtctc tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg
     8701 aatgggttga atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc
     8761 ggctccaatt atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca
     8821 gatctctacc acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat
     8881 caatgctccc cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat
     8941 ctgcacttcc tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt
     9001 tcgcacttcc tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt
     9061 ctgtacttcc gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg
     9121 aagtaatacc agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc
     9181 agaaccggat cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca
     9241 atcttagaga aaatcaacta tctcccagaa taatgcaaac tttgttacaa caaaccgccc
     9301 aaccggacta ccacggccca cggatttact tctccatgag tgacggacaa cagaatacac
     9361 tccatcgccc cctggctgat gccgtgacag catggttccc ggaaaacaaa caatctgatg
     9421 tatcacagat atggcatgct tttgaacatg aagagcatgc caacaccttt tccgcgttcc
     9481 ttgaccgcct ttccgatacc gtctctgcac gcaatacctc cggattccgt gaacaggtcg
     9541 ctgcatggct ggaaaaactc agtacctctg cagagcttcg acagcagtct ttcgctgttg
     9601 ctgctgatgc cactga
//
LOCUS       DAJTUM010000129         9595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTUM010000129 DAJTUM010000000
VERSION     DAJTUM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO471_003558"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO471_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6244680.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO471_003559"
     CDS             complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO471_003559"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6244681.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO471_003560"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO471_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6244682.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2403..2606)
                     /locus_tag="OO471_003561"
     CDS             complement(2403..2606)
                     /locus_tag="OO471_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244683.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO471_003562"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO471_003562"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6244684.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO471_003563"
     CDS             complement(3802..4014)
                     /locus_tag="OO471_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6244685.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO471_003564"
     CDS             4274..4582
                     /locus_tag="OO471_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6244686.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO471_003565"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO471_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6244687.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO471_003566"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO471_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6244688.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO471_003567"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO471_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6244689.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO471_003568"
     CDS             complement(8478..8981)
                     /locus_tag="OO471_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6244690.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9069..>9595)
                     /locus_tag="OO471_003569"
     CDS             complement(9069..>9595)
                     /locus_tag="OO471_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6244691.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagcttgtt gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
      901 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacat cggttgacca ggcggttgct gatgcagccg agtcggtgac
     1501 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacaa ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ctgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttgccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagtagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggt gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt
     9061 gaagaatatt accaagtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     9121 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     9181 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     9241 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     9301 gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     9361 ctgtcgtaag gctgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     9421 tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     9481 tcgtaaccgg ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     9541 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagc
//
LOCUS       DAJTUM010000130         9567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTUM010000130 DAJTUM010000000
VERSION     DAJTUM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO471_003570"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO471_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6244692.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO471_003571"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO471_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6244693.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO471_003572"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO471_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6244694.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO471_003573"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO471_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6244695.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO471_003574"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO471_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6244696.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO471_003575"
     CDS             complement(6031..6360)
                     /locus_tag="OO471_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6244697.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO471_003576"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO471_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6244698.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO471_003577"
     CDS             7998..8171
                     /locus_tag="OO471_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6244699.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO471_003578"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO471_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6244700.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2494 a         2247 c         2388 g         2438 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgtg tgaatgtgtt aaacaat
//
LOCUS       DAJTUM010000131         9496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTUM010000131 DAJTUM010000000
VERSION     DAJTUM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..200)
                     /locus_tag="OO471_003579"
     CDS             complement(<1..200)
                     /locus_tag="OO471_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244701.1"
                     /translation="MCYEYDAYYLYRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGV
                     VCDPYICVNSDGISPELTRKYLG"
     gene            713..1408
                     /gene="minC"
                     /locus_tag="OO471_003580"
     CDS             713..1408
                     /gene="minC"
                     /locus_tag="OO471_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6244702.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1432..2244
                     /gene="minD"
                     /locus_tag="OO471_003581"
     CDS             1432..2244
                     /gene="minD"
                     /locus_tag="OO471_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6244703.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2248..2514
                     /gene="minE"
                     /locus_tag="OO471_003582"
     CDS             2248..2514
                     /gene="minE"
                     /locus_tag="OO471_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6244704.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2886..3230)
                     /locus_tag="OO471_003583"
                     /pseudo
     CDS             complement(2886..3230)
                     /locus_tag="OO471_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3265..3384)
                     /locus_tag="OO471_003584"
     CDS             complement(3265..3384)
                     /locus_tag="OO471_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6244705.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3345..3530)
                     /locus_tag="OO471_003585"
     CDS             complement(3345..3530)
                     /locus_tag="OO471_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244706.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3631..3804
                     /locus_tag="OO471_003586"
     CDS             3631..3804
                     /locus_tag="OO471_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244707.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3806..4150
                     /locus_tag="OO471_003587"
     CDS             3806..4150
                     /locus_tag="OO471_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6244708.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4154..4489
                     /locus_tag="OO471_003588"
     CDS             4154..4489
                     /locus_tag="OO471_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6244709.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4546..6867)
                     /locus_tag="OO471_003589"
     CDS             complement(4546..6867)
                     /locus_tag="OO471_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6244710.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6984..7193)
                     /locus_tag="OO471_003590"
     CDS             complement(6984..7193)
                     /locus_tag="OO471_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244711.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7944..9467)
                     /locus_tag="OO471_003591"
     CDS             complement(7944..9467)
                     /locus_tag="OO471_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6244712.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2665 a         2205 c         2078 g         2548 t
ORIGIN      
        1 ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca aatatacggg
       61 tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc agacgccatt
      121 gatgagaaca tcatccccac acctgacaat ccgatataaa taatccgata taaataatac
      181 gcatcatatt catagcacat cctgataatc caaccgtaca acacttatgc tcatttcgtc
      241 atcaatccgt aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca
      301 gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag ataacgggta
      361 tctgttcttt aatgatgctg caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa
      421 atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca cgattagggt
      481 gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg gttgattgac
      541 aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact tttactctga
      601 ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct tatagtcttc
      661 ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca ggatgtcaaa
      721 cactccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc tgcatgaggc
      781 agaacctaag gttatccatc aggcgctgaa agacaaaatc gctcaggccc ccacattttt
      841 aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa actggtcagc
      901 gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga
      961 tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga
     1021 aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa tgccggtcac
     1081 aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg ctccacaatg
     1141 tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg atgggaacat
     1201 tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac
     1261 gcaaatattt tgtacgaacc tgatggcgga attggtgtcc atcgcaggtg aatactggct
     1321 gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt tagtcgaaaa
     1381 cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc tatggcacgc
     1441 attattgttg ttacttcggg taaagggggt gttggtaaga caacctccag cgcggccatc
     1501 gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga tatcggcctg
     1561 cgtaatctcg acctgattat gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc
     1621 attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc
     1681 tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga aggggtcgcc
     1741 aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga ctccccggca
     1801 gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat tattaccacc
     1861 aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct ggcgtcaaaa
     1921 tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt aacgcgctat
     1981 aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct ggagatcctg
     2041 cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc ctctaaccag
     2101 ggtgaaccgg tcattctcga cattaacgcc gatgcgggta aagcctacgc agataccgta
     2161 gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa gaaaggcttc
     2221 ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct ctcgcggaag
     2281 aagaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc
     2341 agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga ggtcatttgt
     2401 aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa agatggcgat
     2461 atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa ataagcccgc
     2521 tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt tcatccctct
     2581 catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg ctatttccac
     2641 ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta tgcctggttg
     2701 agtaaataac catgttatta gtgtgtatat actgggagat gataaccccg caatttcatt
     2761 attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt tttttgcccc
     2821 agaattttct ctttcaacat cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt
     2881 gatggtcaga acgaccagtt cacaccagcc accgcgttcc acggggactc cacaccggca
     2941 ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga ggctttaata
     3001 cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc gtcactattc
     3061 actttcaccc gattatcatc gacaaattct ttgcgcacag ccgccttcag gcagggttca
     3121 atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat
     3181 atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata gtagcgtcta
     3241 agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg
     3301 aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg
     3361 atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt agttggataa
     3421 aaaatcactc ttttcagata atactgtcgt acgcttatct ttagacaatg aggccgtcgg
     3481 aaaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca
     3541 cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg
     3601 acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc
     3661 attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat
     3721 aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca
     3781 acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc
     3841 agcattgttc tgctccacgc cagcgatggc ggatatgaac cgcaccacga aaggtgcatt
     3901 gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc
     3961 agctgttggc gctggtgtta gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg
     4021 taaaggtgca aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg
     4081 cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa
     4141 aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc
     4201 accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat
     4261 tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt
     4321 tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga
     4381 actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct
     4441 gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg
     4501 taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga acttatattt
     4561 caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt
     4621 cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa
     4681 attactcacc ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact
     4741 gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt
     4801 tttctcctga gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc
     4861 gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga cgcccatcca
     4921 gatagcctgt gcctgtggct ggaagatcca cgtatatttc gccgcttcca gaccaaagga
     4981 tttattcagg gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt tcgcgctata
     5041 ggattcatag gctaattcat cccctttcac ggagttttta aaccagctat attgcgccca
     5101 ggagtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg catacagccc
     5161 tgcgctatag cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttaa
     5221 gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac tatcggtaaa
     5281 accaccggtt aacagatcgc cccctaactg cgagacgtag cgatgcgagg tcgttctcaa
     5341 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct
     5401 tgaacgctcc tgttcagtta caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag
     5461 acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc cagcttcggg
     5521 gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt ttgtttcacc
     5581 gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt cggtattacc
     5641 tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa taaattcccc
     5701 atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag ctccgctgcc
     5761 tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa taacgacttt
     5821 atccgtagct gaattatcat catccaattc agttctgaga tgcaatacgc cattattacc
     5881 aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat ctgcccggga
     5941 aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag ctttaatatc
     6001 ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttatcatctg ttgaaatatt
     6061 agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt taatattatt
     6121 gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt tactcgtctc
     6181 atctttatgg tagaggattt catttgctga ccgtagtgag ctaccagata aaatatctac
     6241 cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct gatgcgcgac
     6301 tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcaa tcgcggcgtc
     6361 aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc gggcagcgat
     6421 ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt
     6481 ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg cggcataact
     6541 ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta tggcattatt
     6601 ttcattactt cctgtatcgg cagtactttc ttcatcaaga gaagatacac acgcacacgc
     6661 gcttccggga gatgcacacg caagaacgcc gactgcgtga ttacccgcag taacaataaa
     6721 tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga ctcttattat
     6781 ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt aatcccccgt
     6841 tgtgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac ttacgattgc
     6901 gctagctgtt aatgctgggc ttagaggact attgtcagaa ggatataaac cgcccgttgt
     6961 tacaatgacg ccattgttaa tggttaactt actaccctct cctgcaacgg atacccgtaa
     7021 cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt tccatctttt
     7081 attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata tgcattaccc
     7141 accgccagag caataagtcc caaactaaac ccggggagtt ttttcagttt catagattaa
     7201 ctatccattc attggattaa caaacattcc tggtcaaata acctatgagc tcagattacc
     7261 aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata aacagcgaca
     7321 atatataaat gataaataag tcggcattat catttttcac cttattttca tgacattgat
     7381 cactttgggg tgactatttt gtttaacaaa taactcttcg acatcaaaat tattaatagt
     7441 tgccatggta attatttcgg tgatatataa aaacaactgt gataacacca aggttgacaa
     7501 ctatttgcgc aataattcct ttccttcaat gatccaggcg ttcactgcca gcaacccatt
     7561 cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg gctaacgagt
     7621 ctttctctta aatcgattaa ccagattgat cgccagccaa acaagggcat taagcaaatt
     7681 tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga tgaccaggag
     7741 ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat ccagattatt
     7801 actattattc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt atagcaaaca
     7861 aaataatgaa caatagtaac aagaggcaac gagctaatta gctgtctata attacaccgc
     7921 actatggcct tactgatcaa ttttcactca accacaacct tcaccttcgg tttagaaagg
     7981 ataattttga ccaggtcgat gtatgtaaca ggtttataga aataataacc ctgctgaaat
     8041 gtgatattat tttggttcag atagccaagt tgttctttcg tttcgacacc ttcagcgacg
     8101 atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg ggttgattct
     8161 gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt tgatataatc aatatgcagg
     8221 tcatgaagat aagaaagccc tgagtagcca gtaccgaaat catccagcgc gatgacaaaa
     8281 ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt caatggctca
     8341 cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc acggaaattt
     8401 aaacactcgt cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt aattccaata
     8461 tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac ctgattcatc
     8521 aggctttgtg taagcggaac gattaatccg gatttttcag caagtggaat aaatgccgcg
     8581 ggtgaaatat atccaccgtg aggttgtttc cagcgggcta acacctcaac tccccgcaat
     8641 gtcccttccc gaccatttac cacaggttgg taaaaaggga cgatctcccc attatttatt
     8701 gcccgtcgca ggatctcttc aggcgttgcg cttttattga aatatctatc aagcaggaag
     8761 gcagcggcgc tggcgattaa cagaataaat attaaaatcc caaagccttt atcgataagt
     8821 ctgtagaagc taaagagtgg cggcgggtta aagataatag tgaaatgata ttttgtggag
     8881 tgaaccttct cggtataggg ataatgtcca ttaagcgtca tcacatcagc cgttggtcca
     8941 ataatgtcat ccgccacgcg taatacatac cttacccctt tcaagggtac attaagcgcc
     9001 ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg tatctgcaaa ttggctctga
     9061 tagagcagga taggtaatct attaacggtg tcgattgccg gagccaacag taaattactg
     9121 tccgggacaa caggaatacg agacagcagg acccgattcc caggcaggga tgtacaccag
     9181 actattccct gtttgagaat gatgattgtg cgcaggtgag gtttcagagc tgcttcagtg
     9241 ccaagctgat attgctcctc gaggtcgcat tccttcccgg caatgttaac cgctgtttga
     9301 gtcgcctgtg acgcttcttc aagtatatga ttgatattgt tggcggcata tcttgctccc
     9361 gccaggtact ctgcttttgc ggaataccag agctggatgt tcaaaatagc gacaccggta
     9421 ataaacaagc atatcgcaac gaggatgggt atgagtgtat tgcgcatttg cttgtcccgg
     9481 ccaaagggtt taatac
//
LOCUS       DAJTUM010000132         9440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTUM010000132 DAJTUM010000000
VERSION     DAJTUM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..434)
                     /locus_tag="OO471_003592"
     CDS             complement(<1..434)
                     /locus_tag="OO471_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6244713.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGV"
     gene            complement(626..2908)
                     /gene="pflB"
                     /locus_tag="OO471_003593"
     CDS             complement(626..2908)
                     /gene="pflB"
                     /locus_tag="OO471_003593"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6244714.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(2963..3820)
                     /gene="focA"
                     /locus_tag="OO471_003594"
     CDS             complement(2963..3820)
                     /gene="focA"
                     /locus_tag="OO471_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6244715.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4226..5986)
                     /locus_tag="OO471_003595"
     CDS             complement(4226..5986)
                     /locus_tag="OO471_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6244716.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6116..6742
                     /locus_tag="OO471_003596"
                     /pseudo
     CDS             6116..6742
                     /locus_tag="OO471_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            6941..8029
                     /gene="serC"
                     /locus_tag="OO471_003597"
     CDS             6941..8029
                     /gene="serC"
                     /locus_tag="OO471_003597"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6244717.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8100..9383
                     /gene="aroA"
                     /locus_tag="OO471_003598"
     CDS             8100..9383
                     /gene="aroA"
                     /locus_tag="OO471_003598"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6244718.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2398 a         2261 c         2374 g         2407 t
ORIGIN      
        1 actccaacca gattttggtg gatctcgtcg ttcatctgtt tgagatcgag cattaccagg
       61 tcggttactt ccagcagttc atcaatcacc ggatcgtaac gacgaacaaa accgttggta
      121 tccagacagg tatgaatgcc ttctttttta caggcgcgga accagtcacg aacaaactca
      181 gcttgcagga ttgcttcacc gccggatgcg gtaacgccgc cgccggaagc gttcataaag
      241 tggcgatagg tcaccacttc cttcatcaaa tcttcaacgg taacttcttt accgccatgc
      301 gtgtcccagg tgtcgcggtt atgacaatac aggcagcgca tcaggcagcc ctggaaaaag
      361 gtgataaagc gaatacctgg gccgtctacg gttccacagg attcaaagga gtgaatgcga
      421 ccaataactg acattgcggt gtttctccag atgtggccca tctgaggccg tgttggtgcg
      481 cagctcgaag gctacgtcga gtctgttttg gcagtcacct taaagtatag atagctgaca
      541 aaaaaggctc tcgtgctaaa aaaggcccca ctttcgtgga gcctttattg tacgcttttt
      601 actgtacgat ttcagtcaaa tctaattaca tagattgagt gaaggtacga gtaataacgt
      661 cctgctgctg ttctttagtc agcgagttga aacgtactgc gtagccagat acacggatgg
      721 tcagctgcgg atatttttcc gggttttcca tcgcgtcgag cagcatttca cggttcatca
      781 cgttaacgtt caggtgctga ccaccttcga tggatgcttc gtggtggaag taaccatcca
      841 tcagaccagc caggttggtc ttacgaactt cgtcgtcttt acccagtgcg ttcggaacga
      901 tagagaaggt gtaggagata ccatctttag cgtaagcaaa cggcagttta gcaacggaag
      961 tcagagacgc tacagcacct ttctggtcac gaccgtgcat cgggttagca cccggtccga
     1021 acggcgcgcc agcacgacga ccgtctgggg tgttaccagt tttcttacca tacacaacgt
     1081 tagaagtgat ggtcagaaca gactgagtcg ggatagcgtc acggtaggtg tgcagtttct
     1141 gaattttctt catgaaacgt tctaccaggt caacagccag gtcatctaca cgcggatcat
     1201 tgttaccaaa ctgcgggtat tcgccttcga tttcgaagtc gatagccaga ccgtcttcgt
     1261 cacgaatcgg tttaactttc gcatatttga ttgcagacag ggagtcagca gcaacggaca
     1321 gaccagcgat accacacgcc atggtgcgga taacgtcacg gtcgtgcagc gccatcagag
     1381 aggcttcgta gctgtacttg tcgtgcatgt agtggatgat gttcagtgca gtgatgtact
     1441 gtttagccag ccagtccatg aagtgatcca tgcgctccat cacttcatcg tagctcagga
     1501 gatcgccttt gatcggttca gacttcggac caacctgcat tttcagtttt tcgtcaacgc
     1561 cgccgttgat tgcgtacagc atggttttcg ccaggtttgc acgcgcaccg aagaactgca
     1621 tttgtttacc aacgatcatc gggcttacgc agcaagcgat agcgtagtca tcgttgttga
     1681 agtccggacg catcaggtca tcgttctcat actgcagaga agaggtgtcg atggacactt
     1741 tagcggcgaa tttcttgaag ttcagcggca gtttttcaga ccacagaatg gtcatgttcg
     1801 gttccggaga cggccccata gtgtacaggg tgttcaggaa acggaagctg tttttggtaa
     1861 ccagggtacg accatcgagg cccataccac cgatagattc ggttgcccag attgggtcgc
     1921 cagagaacag ttcatcgtat tccggagtac gcaggaagcg aaccatacgc agtttcatga
     1981 ccaggtggtc aaccatttcc tgcgcttctt gttcggtgat cttgccagct ttcaggtcac
     2041 gttcgatgta cacatccagg aaggtggagg tacgaccgaa ggacattgca gcaccgttct
     2101 gagacttaac agcagccagg tagccgaagt aagtccactg gatagcttcc tgagcgttgg
     2161 tagccggacc agagatgtcg tagccgtatt tcgcagccat ttctttcatc tgacccagag
     2221 cgcggtgctg ttcagcgatt tcttcgcgca gacggatagt ctgttccagg tttacgccgt
     2281 tttccagatc agcctgtaga gaggtgaact gagcgtattt gtctttcatc aggtagtcga
     2341 taccgtacag cgcaacgcga cggtagtcac cgatgatacg gccacggcca taagcatctg
     2401 gcagaccggt cagaacaccg gatttacggc aacgcaggat gtccggagtg taaacgtcga
     2461 acacgccctg gttgtgagtt ttacggtatt cagtgaagat ttttttgatc atcgggtcca
     2521 gttcgcggtt gtacgctttg caggaacctt cgatcatttt gataccaccg aacgggataa
     2581 gagcacgttt cagcggagct tcagtctgca gaccaacaac tttttccaac gctttattga
     2641 tgtagccagc gtcgtgagag gtgatggtgg aagcaacagc ggtgtcaaaa tcaactggcg
     2701 cgtgagtgcg gttttccagt ttaacacctt ccattacttt gtcccacagg gtggtggtcg
     2761 cttcagtagc gccagccagg aaggactcgt caccctcgta cggagtgtag tttttctgaa
     2821 tgaagtcacg gacgtttact tcattctgcc agtcaccttt ggtaaaacct tcccaggctg
     2881 tggctaactt ttcattaagc tcggacatgt aacacctacc ttcttaagtg gattttttat
     2941 ttactgcgta cttcgacaac cattaatggt gatcgttttc acgcaggtaa atgacccagt
     3001 atgtcaaccc aaccaacaaa ccaccgccga taatattacc gatcgtaacc gggatcaggt
     3061 tatcagtgat gaagttcatc acggtcaggt gagaaaaatt ttccggtgca gaaccgacag
     3121 cggtccagaa ttccggagat gcgaagtcgc ggattacaat acccatcggg atcataaaca
     3181 tgtttgcgat actgtgctca aaaccgctgg caacaaacat cgcgaccggc agcaccataa
     3241 tgaacgcttt gtccatcagg ctgcggccag aataactcat ccatactgcc agacatacca
     3301 tcaggtttgc caggatacca agacagacgg cctcaataaa agtatggtgc actttgtggt
     3361 cggcggtttg taggacgttt agtccccatt gaccatttgc ggtcatatac tcgccggaaa
     3421 gccacattaa aagtacaaac agcagtgcgc cgaccaggtt gccaaaatag acatttagcc
     3481 agtttttagc caactgacac caggtgatgc gcccactcgc cttagcaaca acaatcaaca
     3541 ctgtggaagt aaagagatcg gctccgcaga caacacaaag aatcagcccc agagagaagc
     3601 aaatgccgcc aaccagtttt gccatgccga agggcattgt gcctgtgcca gtggttgctg
     3661 tgatatagaa gacgaatgcg attgagatga aaacaccggc ggtaatcgcc agatagaaag
     3721 tcttaagcgg atgtttcgtt gctttataga cacccgcctc ttcggccact ttggccattg
     3781 cagcaggaag taaaagatca aaagggttgt cagctttcac actaactctc tctttattaa
     3841 gtcggcgacg agatactaac aaagcattat agatgagaaa ttgatataga tcatatctcg
     3901 cctggcttat aggcccgtaa ctcgcatggt ttttatgcaa atacggagta actatttgat
     3961 tatccaaata aaaataaatt ttaaaaatta acaaatgagt tgaatttttt ccgcatcctc
     4021 cgctaaaaca gttaattaaa agggagcatc aggcgaataa agtaacaata tcgatcgtat
     4081 ttattaaata caaattaccg atatttaacc ttataattac aattatttta ttaatgcaaa
     4141 tatatgtaaa gcggggcatt aaaaaaacgc cccgtaatat aactcagact aatcattaag
     4201 cctacattgc gtaggctatt tgattttatt ttgcccagaa tgctgctttg gcgcgctgca
     4261 gcttttcgta ggccttcaac aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta
     4321 ccggttgcag gccgtaaaac gccgcttcgc cgctcattgc cgcactggcg gcttctacgg
     4381 catctgcgcc gtacatgcga acaaacgcat tcagatattg cagcggctgg cgatcttctt
     4441 cctgtgccag taataacaac gtttgcaggc agcgataata gttggcgcgt tccggactaa
     4501 atactgatga gttaaactcc atcgtccatt cggtccagac cagagcctgt tccagatcgc
     4561 caccagccag cgccagcatg gcttttaatt caccgatacg cagggtgtac caaccgttat
     4621 ccgacccggt cgccagaccc aacagctcac gcacgcgggt aaagtcatca aaaccttctt
     4681 catccagttg ctcgatgagg ttcaggtaat cttctttttc ccactcgctg cctggtagcg
     4741 aaagaatcgt ttcacgtaaa tggctgccca tactgttatt cgcgagccac agatcttcag
     4801 ccggataaat atcggacatg ccaggcacga taatacggca agcataaacg cccagatgct
     4861 cgtaatcggc aatataaact tctttatctt ctttgttgaa gatagccatc agcgtagcaa
     4921 actcttcttc cgtggtgccg gagaaattcc agtccacaaa cggataatcg gcatcctgct
     4981 tgaacaggtc ccaggagatt aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat
     5041 gttcagcgac ttcttcatca tcgaaggttg gcggagtaaa cacatccaaa tctttcaggc
     5101 cacgaccttg cagcagctcg gtcacggtac gttccagtgc tacgccaaaa tcaggatgcg
     5161 caccgaaaga ggcaaagcag gtgccgtttg caggattaaa cagtaccacg caaatcaccg
     5221 gatactggcc gccaagggaa ccatcatatg cgaagatcgg gaaaccttct gtttccagtg
     5281 tttcgatcgc ttcaaccacc gctgggtaac gcgccagcac gtctgccggg atttccggca
     5341 agctgatgct ttcagcaata atgcggtttt tcacgtagcg ttcgaaaact tcggacaacc
     5401 cctgaacgcg tgcttcgttg cgggtattgc cagcggacat accgttagaa acgtacaggt
     5461 taccaatgat attcatcgga atataaacgg tctgattgtc ggactggcgc gtaaacggca
     5521 gaccgcaaat accacgatct tcgttaccgg attgtaggtc aatcagcata ctgccggtca
     5581 attcattctc cggatcgtaa aatgcgcgca gacggtcatc gagcagccct tctggcacat
     5641 cgtcattttc gctcagtggg aaccattttt cgttgggata atgcacaaat ggaccgttgg
     5701 cgatggtttc gcccagccag aaatcagcaa aaaagtagtt ggttgagaga cgctcgaaat
     5761 attcaccgag tgcagaagcc agcgccgctt tcttggttgc gcctttaccg ttggtaaaac
     5821 acagtgcgca ctctttgtcg cgaatatgta cagaccagac gttaggcacg ggattcagcc
     5881 aggaggcctc ttcaatctga aagccgaggt ctgaaagttt ttgctggaag cgagcgatgg
     5941 aatcttccag agcggcatct ttgccgggga taaatgtttg cgtcatgaaa atcactttag
     6001 tcgtacggaa agcgcgcaat aatacgggtt ttatctcaaa ggcgctatca ccgccgccat
     6061 gccgatgaac tgttgactat gcttttagcg gataaaccac gttaagagca taaaaatgaa
     6121 ggcgttcgat ctccaccgta tggcatttga taaagtgcct tttgatttcc ttggcgaagt
     6181 tgcactacgt agtctttata cctttgtact ggttttttgt tcctcaaaat gaccggaaga
     6241 cgcggtgtgc gacagatgtc gctgtttgaa gtgttaatca ttctcacgct gggttcagcg
     6301 gcgggagatg tggcgtttta tgatgatgtg ccgatggtgc cggtacttat cgtctttatc
     6361 actctggcgt tgttataccg cctggtaatg tgatggcgag ctggcctggt cgaaactcaa
     6421 taactccaac atgacggaat ttgagttctt tatggagcta cgattgcgtg gcgtggagca
     6481 gctggggcag gtacgtctgg cgattctcga aaccaacggg caaatcagtg tctatttctt
     6541 tgaagatgac aaggtgaaac cgggtttact tattttaccc agtgattgta cgcagcgtta
     6601 caaagtggtg ccggagtcgg cggactatgc ctgcattcgt tgtagtgaaa tcattcatat
     6661 gaacgcgggg gaaaaacaat tatgtccgcg ctgtgcaaat ccagaatgga cgaaggcaag
     6721 tcgggcaaaa cgggtgacct gacagtaaaa acatcggctt tttgctaata atccgagaga
     6781 ttcttttgtg tgatgcaagc cacatttttg ccctcaacgg ttttactcat tgcgatgtgt
     6841 gtcactgaat gataaaaccg atagccacag gaataatgta ttacctgtgg tcgcaatcga
     6901 ttgaccgcgg gttaatagca acgcaacgtg gtgaggggaa atggctcaaa tcttcaattt
     6961 tagttctggt ccggcaatgc taccggcaga ggtgcttaaa caggctcaac aggaactgcg
     7021 cgactggaac ggtcttggta cgtcggtgat ggaagtgagt caccgtggca aagagttcat
     7081 tcaggttgca gaggaagccg agaaggattt tcgcgatctt cttaatgtcc cctccaacta
     7141 caaggtatta ttctgccatg gcggtggtcg cggtcagttt gctgcggtac cgctgaatat
     7201 tctcggtgat aaaaccaccg cagattatgt tgatgccggt tactgggcgg caagtgccat
     7261 taaagaagcg aaaaaatact gcacgcctaa tgtctttgac gccaaagtga ctgttgatgg
     7321 tctgcgcgcg gttaagccaa tgcgtgaatg gcaactctct gataatgcgg cttatatgca
     7381 ttactgcccg aatgaaacca tcgacggtat cgccatcgac gagacgccag acttcggcaa
     7441 agatgtggtg gtcgccgccg acttctcttc aaccattctt tcccgtccga ttgacgtcag
     7501 ccgttatggt gtaatttacg ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat
     7561 cgtcatcgtt cgtgaagatt tgctgggcaa agcgaatatc gcgtgtccgt cgattctgga
     7621 ttattccatc ctcaacgata acggctccat gtttaacacg ccgccgacat ttgcctggta
     7681 tctatctggt ctggtcttta aatggctgaa agcgaacggc ggtgtagctg aaatggataa
     7741 aatcaatcag caaaaagcag aactgctata tggggtgatt gataacagcg atttctaccg
     7801 caatgacgtg gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt tggcggacag
     7861 tgcgcttgac aaattgttcc ttgaagagtc ttttgctgca ggccttcatg cactgaaagg
     7921 acaccgtgtg gtcggcggaa tgcgcgcgtc tatttataat gccatgccgc tggaaggcgt
     7981 taaagcgctg acagacttca tggttgagtt cgaacgccgt cacggttaat gccgaaattt
     8041 tgcttaatcc ccacagccag cctgtggggt ttttatttct gttgtagaga gttgagttca
     8101 tggaatccct gacgttacaa cccatcgctc gtgtcgatgg cactattaat ctgcccggtt
     8161 ccaagagcgt ttctaaccgc gctttattgc ttgcggcatt agcacacggc aaaacagtat
     8221 taaccaatct gctggatagc gacgacgtgc gccatatgct gaatgcatta acagcgttag
     8281 ggttaagcta tacgctttca gccgatcgta cgcgttgcga aattatcggt aacggcggtc
     8341 cattacacgc agaaggtgcc ctggagttgt tcctcggtaa cgccggaacg gcaatgcgtc
     8401 cgctggcggc agctctttgt ctggatagca atgatattgt gctgaccggt gagccgcgta
     8461 tgaaagagcg cccgattggt catctggtgg atgcgctgcg cctgggcggg gcgaagatca
     8521 cttacctgga acaagaaaat tatccgccgt tgcgtttaca gggcggcttt actggcggca
     8581 acgttgacgt tgatggctcc gtttccagcc aattcctcac cgcactgtta atgactgcgc
     8641 ctcttgcgcc ggaagatacg gtgattcgta ttaaaggcga tctggtttct aaaccttata
     8701 tcgacatcac actcaatctg atgaagacgt ttggtgttga aattgaaaat cagcactatc
     8761 aacaatttgt cgtaaaaggc gggcagtctt atcagtctcc gggtacttat ttggtcgaag
     8821 gcgatgcatc ttcggcttct tacttcctgg cagcagcagc aatcaaaggc ggcactgtaa
     8881 aagtgaccgg tattggacgt aacagtatgc agggtgatat tcgctttgct gatgtgctgg
     8941 aaaaaatggg cgcgaccatt tgctggggcg atgattatat ttcctgcacg cgtggtgaac
     9001 tgaacgctat tgatatggat atgaaccata ttcctgatgc ggcgatgacc attgccacgg
     9061 cggcgttatt tgcaaaaggc accaccacgc tgcgcaatat ctataactgg cgtgttaaag
     9121 agaccgatcg cctgtttgcg atggcaacag aactgcgtaa agtcggcgcg gaagtggaag
     9181 aggggcacga ttacattcgt atcactcctc cggaaaaact gaactttgcc gagatcgcga
     9241 catacaatga tcaccggatg gcgatgtgtt tctcgctggt ggcgttgtca gatacaccag
     9301 tgacgattct tgatcccaaa tgcacggcca aaacatttcc ggattatttc gagcagctgg
     9361 cgcggattag ccaggcagcc tgaatgaaca acgggcaata aatagccaaa tctttcttta
     9421 tcaaaacgtc ggcacattgt
//
LOCUS       DAJTUM010000133         9424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTUM010000133 DAJTUM010000000
VERSION     DAJTUM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..311)
                     /locus_tag="OO471_003599"
     CDS             complement(<1..311)
                     /locus_tag="OO471_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6244719.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
     gene            complement(492..1868)
                     /gene="trkA"
                     /locus_tag="OO471_003600"
     CDS             complement(492..1868)
                     /gene="trkA"
                     /locus_tag="OO471_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6244720.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1890..3179)
                     /gene="rsmB"
                     /locus_tag="OO471_003601"
     CDS             complement(1890..3179)
                     /gene="rsmB"
                     /locus_tag="OO471_003601"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6244721.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(3225..4172)
                     /gene="fmt"
                     /locus_tag="OO471_003602"
     CDS             complement(3225..4172)
                     /gene="fmt"
                     /locus_tag="OO471_003602"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6244722.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(4187..4696)
                     /gene="def"
                     /locus_tag="OO471_003603"
     CDS             complement(4187..4696)
                     /gene="def"
                     /locus_tag="OO471_003603"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6244723.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4861..5985
                     /gene="dprA"
                     /locus_tag="OO471_003604"
     CDS             4861..5985
                     /gene="dprA"
                     /locus_tag="OO471_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6244724.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5957..6430
                     /locus_tag="OO471_003605"
     CDS             5957..6430
                     /locus_tag="OO471_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6244725.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6459..7001
                     /locus_tag="OO471_003606"
     CDS             6459..7001
                     /locus_tag="OO471_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6244726.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            7006..7578
                     /gene="tsaC"
                     /locus_tag="OO471_003607"
     CDS             7006..7578
                     /gene="tsaC"
                     /locus_tag="OO471_003607"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6244727.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7583..8401
                     /gene="aroE"
                     /locus_tag="OO471_003608"
     CDS             7583..8401
                     /gene="aroE"
                     /locus_tag="OO471_003608"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6244728.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8398..8655
                     /locus_tag="OO471_003609"
     CDS             8398..8655
                     /locus_tag="OO471_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6244729.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8631..9185)
                     /locus_tag="OO471_003610"
     CDS             complement(8631..9185)
                     /locus_tag="OO471_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6244730.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2359 a         2272 c         2358 g         2435 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa gacgtcgctg atggggcgct tacgtctaac aaatgttaag ccattttcct
      421 gccaggaatg aatttaatta atccttgata atcaaaggga aataataagg cgtcattaga
      481 cgccttatta attacaagaa gaaagggctt ggctggaaga gtctttcgac gtcggtaata
      541 aactttttat ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg cagattgtca
      601 ttggcaatca tcacgtcgtt tccacgtacc accgctccaa taatcgttcc tggcggtagc
      661 ttgatttcgt caatgactct gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg
      721 gcttcaatag cttctgctac gccgcggcgc aatgaggaaa caccaacaat atctgctttt
      781 cgcacatggc taagcaacgc agaaatagtt gcttgttgtg gtgaaattgc aatatcgatc
      841 acgctcccct gaaccagatc cacataagcg cgacgctgga tcaataccat cacctttttc
      901 gcacccatac gtttggcaag catggcggac atgatattgg cctcgtcatc gttggtgacg
      961 gcaataaaca gatcaacttg atcgatatgt tcttcggcca gcagttcttg atccgacgca
     1021 tcaccaaaaa agacgatcgt attctgcaac ttttccgcca gttcggcagc gcgctgctga
     1081 ttacgttcga tgagtttaac gctgtaatct ttttccagac gacgcgccag ccctgcaccg
     1141 atattaccgc caccaaccag catgatccgt ttatacggtt tttccagtcg ctgtaattca
     1201 ctcatcaccg cgcggatatg ctgtgaagcg gcaataaaga acacttcatc accagcttca
     1261 acaatggtcg aaccttgtgg acgaatgggg cgatcgtggc ggaaaattgc tgccacacga
     1321 gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg cattaccaat cagcgggccg
     1381 ccataatagg ctttaactac agcgaggctg actttaccct cagcgaagtt caccacctgc
     1441 aatgcgcctg gatactcaat cagtcggtaa atattatcga taaccaactg ctctggtgcg
     1501 atcagatgat caatcggtac agcatctgaa tgaaatagct tatcggcatc gcgtacgtag
     1561 tctggtgagc ggatgcgagc gatgcgatta ggggtgttga aaagtgagta ggctacctgg
     1621 caggcaacca tattggtttc atctgaactg gttacagcaa ccagcatatc ggcgtcgtcg
     1681 gcacctgcct cccgcaatac gcgtggatga gagccatgcc cctgcacgac tcgcaggtca
     1741 aatttatcct gcaaggtccg cagacgctca ccgttggtat cgacaacagt aatatcgttg
     1801 ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct ggccggcacc cagaatgata
     1861 attttcatca gtcgcgaccc gttatctcat cactttttga ttagcttagc gtaaaagaag
     1921 ccgtcgccct cttcggcacc aggctgattt tgtttacccg gttgctctgg tgttcctgtt
     1981 tcgcaaagtt cggcatcagc ggtacgttgc aaaaaggctt taatctgcag gctattctct
     2041 tccggtaaca ccgaacaggt ggcatagacc agggttccac cggattttaa atgcggccaa
     2101 atggcgtcga gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg atcgcgacgt
     2161 aaccatttaa tatctggatg gcgacgaatc acccccgttg ctgaacaagg cgcatctaat
     2221 aaaatgcgat caaactgttg ctcgccacac cattgggaag ggtaacggcc atcaccttgt
     2281 ttcacggttg ccttcatacc aaggcgtttt aaattgtcgt aaatgcgaga gaggcgctgt
     2341 tcgtcaatat caaccgcaac aacctgcgct tctggtgcca cctcaaggat atgcgttgtt
     2401 ttaccgccgg gggcggcaca aagatccaag atgtgttcac cgttttgtgg cgcaagccag
     2461 gtcatgcaac cttgtgctga tgcatcctga acggtaaccc atccgtcttc aaaaccaggt
     2521 agcgcatgaa caggtgcagg tgtttccagc cgtacagcat cagggtaatc cgcatgcggg
     2581 aaacctttca ttcctgcttc atccagcaat gcaagccagc tgtcgcggga atgatgcgta
     2641 cgattaatac gcagccacat tggcggacgc tggttattgg cttcggcgat ggattgccac
     2701 tgctctggat acgctttttg cagacgcttc agcaaccagg aaggatgcag ataacgtgca
     2761 tcacgggtat taaactcggc taataactct tcttgccgac gctggaactg gcgtaataca
     2821 ccgttaatca accctttaag ttgcggacgc ttaattgcga tagcgccttc aaccgtttca
     2881 gccagcgcag catgaggtgg aatgcgggta taaagcagtt gatacaaacc aaccataatc
     2941 aggtaatgca cagtgcgctg tttgccggtc atcggacggg ccattaactt attaatcagc
     3001 cagtctaact gcgaaagcgt acgcagtacg ccaaagcaca actcttgaag aagtgctttg
     3061 tctttatcgg aaactttttg ctggagcggt ggcagaatgt tgcttaatga ttgcccttgc
     3121 tcgacgactt gttcaacggc ctgggccgcc atgctacgta aattacgttg ttttttcata
     3181 agtataaaaa tgctcggcaa gaccgggctt agaagagtgg actatcagac cagacggttg
     3241 cccggaacaa accattcccg acgagagttc aggaggtctt gcgcgctcat cgctttctta
     3301 cccgcaggtt gtaacgagag caggttcagg atgccatcac cagtcgcaac ctgaatgcct
     3361 tgtttgttgg cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc aatgaccgac
     3421 gctttccaga ctttaaccgg ctgtccttca atttccagcc agctcattgg ccatgggttg
     3481 aaagcgcgaa tgcagcgttc aagctgtgct gccgaaagtg accagtcaat acgcgcttct
     3541 tctttactca acttctcggc gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc
     3601 gtgccgtctg ccaattgttt caacgtggtg ataagccctt gtgggccaag ctctgccagc
     3661 ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg ggcaggagag cttatagagc
     3721 atgtcaccgg tgtctaaacc aacatccatt tgcataatgg ttacaccagt ttctgcatca
     3781 cccgcccata gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag cagtgaacca
     3841 tgaatgttga tacagccaag acgcggcatc tccagcacag ctttaggcag aattaaacca
     3901 taggcgacga cgaccataac atcagcctgc aaatcggcga ccagttgctg gttttcttgt
     3961 ggacgcaggg aaacaggttg aaaaacgggc agaccttctt cctcagccag aactttaatc
     4021 gggctgggca tcagtttttt accgcgtcct gccggtcggt ctggctgggt gaacacgcca
     4081 acgacgttat gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta
     4141 cccgcaaaaa taatacgtag tgattctgac acgttagttc ttatccttaa gcccgggctt
     4201 tcagacgatc cagtttttca actttctgac gaattcgttg ttgtttcagc ggtgacagat
     4261 aatccataaa cagtttgcca accaggtgat ccatctcatg ctgaatacag atagctaaca
     4321 gaccctctgc ttccagttca aatggtttac cgtcgcggtc aagggcgcga attttaactt
     4381 tctctgcgcg cggcactaaa gcacgttgtt cagggatcga caggcaacct tcttcaatgc
     4441 ctgtttcgcc gcttttttct aaaagctccg gattgattaa caccagccgt tcgtcacggt
     4501 tttccgaaac atcaataaca atgatacgtt gatggatatc aacctgggtt gccgccaggc
     4561 caataccttc ttctgcgtac atcgtctcga acatatcatc gacgatacgc tgaatttctg
     4621 cattcacttc ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca
     4681 cttgcaaaac tgacataaat ctccagagat gtgttcagga gttagaaaga ttatttcttc
     4741 tattctagac aaatccccct ctgattgaca gcatcacttg attgacagca tcacttgatt
     4801 gacagcatca ctgaccaatc gcaaagattg ctaaggcttg cttatggcag ggagataagg
     4861 atggtcgata tagaaatttg gctgcgttta atgagcatca gcagcttgta cggcgatgat
     4921 atggtccgta tcgctcactg gctggcaaaa cagtcgcata ttgatgcggt tgtattgcag
     4981 caaacagggc ttacattgcg gcaggcacaa cgctttcttt catttccacg aaagagtatc
     5041 gaaagctcac tttgttggtt ggagcaaccc aaccatcatt taatccctgc ggacagcgaa
     5101 ttttatcctc ctcaacttca ggcgacgaca gattaccccg gcgcactgtt tgttgaagga
     5161 gaactgcacg cgctgcattc atttcagctt gccgtagtgg ggagtcgggc gcattcatgg
     5221 tatggcgagc gatgggggcg attattttgc gaaactctgg cgacgtgtgg agtgacaatt
     5281 acgagtggac tggcgcgtgg aatcgatggt gtggtgcata aagcggcctt acaggtaaat
     5341 ggcgtcagca ttgctgtatt ggggaatgga cttaatacca ttcatccgcg tcgccatgcc
     5401 cgactggctg ccagtctgtt tgaacagggt ggcgctctcg tctcggaatt tcccctcgat
     5461 gttccacccc ttgcttacaa tttcccacga agaaatcgca ttatcagtgg tctaagtaaa
     5521 ggtgtactgg tggtggaagc ggctttgcgc agtggttcgc tggtgacagc acgttgtgcg
     5581 cttgagcagg ggcgtgaagt ttttgccttg ccaggtccaa tagggaatcc gggaagcgaa
     5641 gggcctcact ggttaataaa acaaggtgcg attcttgtga cggaaccgga agaaattctg
     5701 gaaaacttgc aatttggatt gcactggttg ccagacgccc ctgaaaattc attttattca
     5761 ccagatcagc aagacgtggc attgccattt cctgagctcc tggctaacgt aggagatgag
     5821 gtaacacctg ttgacgtcgt cgctgaacgt gccggccaac ctgtgccaga ggtagttact
     5881 caactactcg aactggagtt agcaggatgg atcgcagctg tacccggcgg ctatgtccga
     5941 ttgaggaggg catgccatgt tcgacgtact aatgtatttg tttgaaacct atattcacac
     6001 tgaagctgag ttgcgtgtgg atcaagacaa acttgaacag gatcttaccg acgcaggttt
     6061 tgagcgagaa gatatctaca atgccctgct atggctggaa aaacttgctg attatcagga
     6121 agggttggca gaaccgatgc aactggcctc ggatcctctc tccatgcgta tttatacacc
     6181 ggaagagtgt gaacgactgg atgccagctg ccgtgggttt ctgcttttcc ttgagcagat
     6241 tcaggtgctc aaccttgaaa ctcgtgaaat ggtgatagag cgagtgctcg cgctggataa
     6301 cgcagagttc gaactggatg atctgaaatg ggtgatcctg atggtgttgt tcaacatccc
     6361 gggctgcgaa aatgcgtacc agcaaatgga agaattactc tttgaagtga atgaaggtat
     6421 gctgcattaa tcatttcttt aattcagcat aagttgttat ggcaaaatca gcactgttca
     6481 cggtgcgtaa taatgagtcc tgcccaaagt gcggggctga actggttatt cgatccggga
     6541 aacacggtcc gtttcttgga tgctcacagt atccggcgtg tgactacgtc cgtcctctga
     6601 aatcttcagc ggatggacat attgtcaaag ttctggaggg gcaggtttgc cccgtatgtg
     6661 gcgcaaatct ggtattacgc cagggacgct ttggtatgtt tattggttgc agtaactacc
     6721 ctgaatgcga acataccgaa cttatcgata aaccggacga aacagcaatt acatgccccc
     6781 aatgtcggac gggccatctg gtccagcgcc gctcccgtta tggcaaaaca tttcactcct
     6841 gtgatcgcta cccggagtgt caatttgcca ttaacttcaa accaatagct ggagaatgcc
     6901 ctgagtgtca ttatccgcta ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt
     6961 gtgccagtaa acaatgtgga aagccggttt cggcggaata ataacgtgaa taataacctg
     7021 caaggagacg ctatcgcagc tgcgatagat gtcctcaatg aaaaacgtgt catcgcctat
     7081 ccaacggaag ccgttttcgg tgtcgggtgc gatcctgata gcgaaacagc agtgatgaga
     7141 ctgttggagt taaaacagcg tccggttgat aaggggctga ttttaatcgc ggcaaattac
     7201 gagcagctta aaccctatat tgatgacacc atgttgactg acgtgcagcg tgaaaccatt
     7261 ttttcctgct ggccaggtcc tgtcaccttt gtctttcccg cgcctgcgac aacaccgcgc
     7321 tggttgacgg gccgctttga ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt
     7381 gctttgtgcc aggcttatgg taaaccgctg gtttctacca gtgccaactt gagtggattg
     7441 ccaccttgtc gaacagtaga cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg
     7501 cctggtgaaa cgggggggcg tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa
     7561 ctgtttcgac aggggtaaca taatggaaac ctatgctgtt tttggtaatc cgatagccca
     7621 cagcaaatcg ccattcattc atcagcaatt tgctcagcaa ctgaatattg aacatcccta
     7681 tgggcgcgtg ttggcaccca tcaatgattt catcaacaca ctgaacgctt tctttcgtgc
     7741 tggtggtaaa ggtgcgaatg tgacggtgcc ttttaaagaa gaggcttttg ccagagcgga
     7801 tgagcttact gaacgggcag cgttggctgg tgcagttaat accctcaagc ggttagaaga
     7861 tggacgcctg ctgggtgaca ataccgatgg tgtaggcttg ttaagcgatc tggaacgtct
     7921 gtcttttatc cgccctggtt tacgtattct gcttatcggc gctggtggag catctcgcgg
     7981 cgtactactg ccacttcttt ccctggactg tgcggtgaca atcactaatc ggacggtatc
     8041 ccgcgcggaa gagttggcta aattgtttgc gcacactggc agtattcagg cgttgggtat
     8101 ggacgaactg gaaggtcatg agtttgatct cattattaat gcaacatcca gtggcatcag
     8161 tggtgatatt ccggcgatcc cgtcatcgct cattcatcca ggcatttatt gctatgacat
     8221 gttctatcag aaaggaaaaa ctccttttct ggcatggtgt gagcagcgag gctcaaagcg
     8281 taatgctgat ggtttaggaa tgctggtggc acaggcggct catgcctttc ttctctggca
     8341 cggtgttctg cctgacgtag aaccagttat aaagcaattg caggaggaat tgtccgcgtg
     8401 aatcaggcca tccagtttcc ggacagggaa gagtgggacg agaataaaaa atgtgtatgt
     8461 tttcccgctc tcgtgaatgg tatgcaactg acatgcgcga tctctggcga gagtctggcg
     8521 tatcgcttta ctggagatac gccagaacag tggttagcga gttttcgtca gcatcgctgg
     8581 gacctggaag aagaagcgga aaacttaatt caggaacaaa gtgaagatga tcaaggctgg
     8641 gtctggttac cctgatccag atattcgtcc ttccatttca cgtaattatt cgcggaatag
     8701 cgtaacccag ccttctcttc atcacttaac gggcggatct gtttgacggg gctaccgaga
     8761 tacagatatc cgctctccag ccgtttattt tgtgggacca gactacccgc accaatcatc
     8821 acatcatctt ctactattgc gccatcaagt aaaattgagc ccatcccaac caaaactcga
     8881 ttgccaatgg tgcagccgtg gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg
     8941 gttaatgggt tgccatctgg gttgtacgag gatttatgag tgacatgcaa catactgcca
     9001 tcctggatat tggtgcgtgc tccgatctgt acataatgta catctccacg aatcacaacg
     9061 agcggccaga tccccacatc atcagccaga cgaacgtcac caatcacgac actgctatcg
     9121 tcgatcatta cgcgctgacc gatttgtgga aaaagatcgc ggtatgggcg taaaacatca
     9181 gacatactta cctcagcaat aaatgattta ctaatgactt tgggggcatt attggccttg
     9241 tgcaagtctt ttagtatgca aaaaagcacc gttttgtgtg cgattgcagc aaaaagggtg
     9301 aaaaaacaac aaacagaaaa gaagatcaaa aaaatacttg tgcaaaatat tgggatccct
     9361 ataatgcgcc tccgttgaga cgacaacgtg aaacacttca caggatggtc ggaacaacga
     9421 agag
//
LOCUS       DAJTUM010000134         9412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTUM010000134 DAJTUM010000000
VERSION     DAJTUM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="OO471_003611"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="OO471_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6244731.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="OO471_003612"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="OO471_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6244732.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO471_003613"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO471_003613"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6244733.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO471_003614"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO471_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6244734.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="OO471_003615"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="OO471_003615"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6244735.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO471_003616"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO471_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6244736.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="OO471_003617"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="OO471_003617"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6244737.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAJTUM010000135         9291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTUM010000135 DAJTUM010000000
VERSION     DAJTUM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO471_003618"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO471_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6244738.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO471_003619"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO471_003619"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6244739.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO471_003620"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO471_003620"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6244740.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO471_003621"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO471_003621"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6244741.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO471_003622"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO471_003622"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6244742.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO471_003623"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO471_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6244743.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO471_003624"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO471_003624"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6244744.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO471_003625"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO471_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6244745.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO471_003626"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO471_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6244746.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO471_003627"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO471_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6244747.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2357 g         2224 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTUM010000136         9243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTUM010000136 DAJTUM010000000
VERSION     DAJTUM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO471_003628"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO471_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6244748.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO471_003629"
     CDS             1828..3147
                     /locus_tag="OO471_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6244749.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO471_003630"
     CDS             3445..3486
                     /locus_tag="OO471_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244750.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO471_003631"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO471_003631"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6244751.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO471_003632"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO471_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO471_003633"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO471_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO471_003634"
     CDS             5815..6711
                     /locus_tag="OO471_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6244752.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO471_003635"
     CDS             6708..7682
                     /locus_tag="OO471_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6244753.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO471_003636"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO471_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO471_003637"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO471_003637"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6244754.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2260 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 aga
//
LOCUS       DAJTUM010000137         9182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTUM010000137 DAJTUM010000000
VERSION     DAJTUM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..894)
                     /locus_tag="OO471_003638"
     CDS             complement(<1..894)
                     /locus_tag="OO471_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6244755.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OO471_003639"
     CDS             954..1232
                     /locus_tag="OO471_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244756.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO471_003640"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO471_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6244757.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO471_003641"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO471_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6244758.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OO471_003642"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OO471_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6244759.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OO471_003643"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OO471_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6244760.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OO471_003644"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OO471_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OO471_003645"
     CDS             complement(4912..5442)
                     /locus_tag="OO471_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6244761.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO471_003646"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO471_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6244762.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OO471_003647"
     CDS             complement(6411..7634)
                     /locus_tag="OO471_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244763.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8215..>9182)
                     /locus_tag="OO471_003648"
     CDS             complement(8215..>9182)
                     /locus_tag="OO471_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6244764.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2678 a         1825 c         1922 g         2757 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
     8161 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
     8221 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
     8281 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc
     8341 ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac
     8401 agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga caacggcgct
     8461 taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg ctgagggaat
     8521 aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt
     8581 aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt
     8641 ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact
     8701 cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc
     8761 tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact
     8821 ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa
     8881 ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa ataaaaaagt
     8941 gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg gtattgtcga
     9001 gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc ctctcccttt
     9061 tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta tcaggctgac
     9121 accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca caaacatctc
     9181 tg
//
LOCUS       DAJTUM010000138         9166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTUM010000138 DAJTUM010000000
VERSION     DAJTUM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..591)
                     /locus_tag="OO471_003649"
     CDS             complement(<1..591)
                     /locus_tag="OO471_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6244765.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO471_003650"
     CDS             complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO471_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6244766.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1420..1959)
                     /locus_tag="OO471_003651"
     CDS             complement(1420..1959)
                     /locus_tag="OO471_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6244767.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO471_003652"
     CDS             complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO471_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6244768.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO471_003653"
     CDS             complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO471_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6244769.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO471_003654"
                     /pseudo
     CDS             complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO471_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6183..6899
                     /gene="nanC"
                     /locus_tag="OO471_003655"
     CDS             6183..6899
                     /gene="nanC"
                     /locus_tag="OO471_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6244770.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6919..8025
                     /gene="nanM"
                     /locus_tag="OO471_003656"
     CDS             6919..8025
                     /gene="nanM"
                     /locus_tag="OO471_003656"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6244771.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8090..9070
                     /locus_tag="OO471_003657"
     CDS             8090..9070
                     /locus_tag="OO471_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6244772.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2686 a         1995 c         1924 g         2561 t
ORIGIN      
        1 ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca
       61 taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag
      121 gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa
      181 tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc
      241 catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt
      301 attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata
      361 cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg
      421 atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc
      481 ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat
      541 atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc
      601 ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta
      661 ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt
      721 aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa
      781 tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt
      841 cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg
      901 atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc
      961 gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt
     1021 catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg
     1081 taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc
     1141 atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc
     1201 attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc
     1261 acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat
     1321 acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact
     1381 cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg ttaaagagaa
     1441 ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc
     1501 gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt
     1561 aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg aggctatccc
     1621 tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac cgtgaaacgc
     1681 cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac
     1741 cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc ccatattgac
     1801 cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa
     1861 ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg
     1921 cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg
     1981 gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga tactgaacct
     2041 tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca taataacgcg
     2101 cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac
     2161 cgtccagcgt cagagcattg cctgttctgt ctaggatctg cacgccaacg tttgttgcac
     2221 tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca attgccgtac
     2281 ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt
     2341 taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctgtc
     2401 ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt
     2461 cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg
     2521 aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt
     2581 cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc
     2641 aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca
     2701 ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat
     2761 attctattat tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa
     2821 gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt
     2881 gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt tttgtcgttt
     2941 tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acagtttggc
     3001 cccaattgtc ttgtgtttat ttgtttttta actttattat caataagtta aatcaaacct
     3061 cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag
     3121 cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg agataatcct
     3181 gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca
     3241 tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga ataatgcgat
     3301 aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg tcggtccggt
     3361 cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca cgctcatcaa
     3421 aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt
     3481 cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc atcccatgcc
     3541 gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct
     3601 gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac
     3661 tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc
     3721 ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa ttacattaaa
     3781 catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg atttgattca
     3841 atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa gaaaaaagtg
     3901 tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt atttattttg
     3961 aagaacataa atcattagat tttgtatggg tatggttaac ttaatgttta ttatgataat
     4021 taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt
     4081 aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcat
     4141 gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg
     4201 tataccagac agtatgacga atattgcgat gcccaagata atcctagata agtcgcgtat
     4261 ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc ggatgaatct
     4321 ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac tgttgccgag
     4381 aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc ggataagaag
     4441 tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc gggtgcgttg
     4501 ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga tcaatatccg
     4561 aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaaatca
     4621 gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt gactcgattt
     4681 cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc gttttccctt
     4741 ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt tttatcctga
     4801 ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca gttcttctga
     4861 gcgaacttaa atggagtagc gatagttact tataataact ttgccataca aacgccatgc
     4921 tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa taataaccct
     4981 catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta acaagtaaaa
     5041 tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc aaatatgaca
     5101 ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt aatttcttag
     5161 tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg ttcgtcagct
     5221 taggccatga gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg
     5281 ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta agccttcctc
     5341 aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc atcctgaaaa
     5401 caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa
     5461 ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta aacccttatt
     5521 ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct aaataaatta
     5581 tttataaaac gtatagttaa aagaaaatct ttagtttttg aagaccaccg caagtgttcg
     5641 tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt gacgcgaatt
     5701 gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac aggtataaag
     5761 atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac atcacttcag
     5821 ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga aaatgagatc
     5881 aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat ataacttacg
     5941 aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta atctggatct
     6001 ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac aaaaaatata
     6061 aaatacagtt atccgttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat
     6121 aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat
     6181 ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa
     6241 tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg
     6301 agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta
     6361 atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg
     6421 ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa
     6481 cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga
     6541 caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag
     6601 acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc
     6661 atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaatgatt
     6721 atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta caataccata
     6781 tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag ggtgtttaca
     6841 acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt
     6901 agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat gatggcttca
     6961 tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga
     7021 gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc atggtacaag
     7081 ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc tggtggaccg
     7141 agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc
     7201 aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc
     7261 aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca tgtgactttt
     7321 gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat
     7381 tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caacgcccac
     7441 tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc
     7501 tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg
     7561 gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga
     7621 acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgat
     7681 cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt
     7741 atatttgctg gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac
     7801 tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg
     7861 aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg
     7921 aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca
     7981 gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg
     8041 gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat
     8101 aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta
     8161 tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt
     8221 agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc
     8281 actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac
     8341 gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact
     8401 gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc
     8461 ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg
     8521 tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact
     8581 ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt
     8641 aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg
     8701 tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg
     8761 cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg
     8821 caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga
     8881 aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg
     8941 ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac
     9001 ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg
     9061 cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca
     9121 acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggct
//
LOCUS       DAJTUM010000139         9165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTUM010000139 DAJTUM010000000
VERSION     DAJTUM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..923
                     /locus_tag="OO471_003658"
     CDS             <1..923
                     /locus_tag="OO471_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6244773.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO471_003659"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO471_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6244774.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO471_003660"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO471_003660"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6244775.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO471_003661"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO471_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6244776.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO471_003662"
     CDS             4446..4805
                     /locus_tag="OO471_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6244777.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO471_003663"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO471_003663"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6244778.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="OO471_003664"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="OO471_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6244779.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="OO471_003665"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="OO471_003665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6244780.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2219 c         2475 g         2378 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgc
//
LOCUS       DAJTUM010000140         8991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTUM010000140 DAJTUM010000000
VERSION     DAJTUM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO471_003666"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO471_003666"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6244781.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO471_003667"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO471_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6244782.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="OO471_003668"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="OO471_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6244783.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO471_003669"
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO471_003669"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6244784.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO471_003670"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO471_003670"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6244785.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="OO471_003671"
     CDS             complement(7822..8133)
                     /locus_tag="OO471_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6244786.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO471_003672"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO471_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6244787.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8991)
                     /gene="csgA"
                     /locus_tag="OO471_003673"
     CDS             complement(8645..>8991)
                     /gene="csgA"
                     /locus_tag="OO471_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6244788.1"
                     /translation="NSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADV
                     GQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQ
                     VGFGNNATAHQY"
BASE COUNT         2249 a         2328 c         2133 g         2281 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtgat
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ttgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctaa ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacatc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtggct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgc aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacta taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc cccaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaagg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaag attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta t
//
LOCUS       DAJTUM010000141         8771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTUM010000141 DAJTUM010000000
VERSION     DAJTUM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO471_003674"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO471_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6244789.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="OO471_003675"
     CDS             complement(984..1814)
                     /locus_tag="OO471_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6244790.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="OO471_003676"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="OO471_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6244791.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="OO471_003677"
     CDS             3240..3590
                     /locus_tag="OO471_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6244792.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTSEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="OO471_003678"
     CDS             3716..4276
                     /locus_tag="OO471_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6244793.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="OO471_003679"
     CDS             4279..7245
                     /locus_tag="OO471_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6244794.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="OO471_003680"
     CDS             7312..7689
                     /locus_tag="OO471_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6244795.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="OO471_003681"
     CDS             complement(7890..8549)
                     /locus_tag="OO471_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6244796.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2479 c         2348 g         1994 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gtcggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DAJTUM010000142         8764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTUM010000142 DAJTUM010000000
VERSION     DAJTUM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            5..436
                     /locus_tag="OO471_003682"
     CDS             5..436
                     /locus_tag="OO471_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6244797.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO471_003683"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO471_003683"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6244798.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO471_003684"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO471_003684"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6244799.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO471_003685"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO471_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6244800.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO471_003686"
     CDS             complement(4438..5145)
                     /locus_tag="OO471_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6244801.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO471_003687"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO471_003687"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6244802.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO471_003688"
     CDS             complement(6003..6206)
                     /locus_tag="OO471_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6244803.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO471_003689"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO471_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8764
                     /locus_tag="OO471_003690"
     CDS             7870..>8764
                     /locus_tag="OO471_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6244804.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2034 a         2388 c         2252 g         2090 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tggcgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 caat
//
LOCUS       DAJTUM010000143         8642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTUM010000143 DAJTUM010000000
VERSION     DAJTUM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO471_003691"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO471_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6244805.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="OO471_003692"
     CDS             complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="OO471_003692"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6244806.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="OO471_003693"
     CDS             complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="OO471_003693"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6244807.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWYFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="OO471_003694"
     CDS             complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="OO471_003694"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6244808.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5267..7813
                     /gene="cyaA"
                     /locus_tag="OO471_003695"
     CDS             5267..7813
                     /gene="cyaA"
                     /locus_tag="OO471_003695"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6244809.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="OO471_003696"
     CDS             complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="OO471_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6244810.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8221..>8642)
                     /locus_tag="OO471_003697"
     CDS             complement(8221..>8642)
                     /locus_tag="OO471_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6244811.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1968 a         2235 c         2222 g         2217 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca ggttacgcac
     2101 gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     2161 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     2221 atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     2281 ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     2341 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2401 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2461 gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt
     2521 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2581 gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2641 aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac ttttcagcaa
     2701 cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2761 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgatctt
     2821 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2881 ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2941 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     3001 ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga tagccaccgc
     3061 gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     3121 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     3181 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     3241 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     3301 gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3361 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3421 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3481 aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac gaccgccatt
     3541 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3601 gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta cggtatgtag
     3661 cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc atttacgatc
     3721 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3781 atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3841 aaactcaatc agcggaaaat accaggccac ctgccccagt gtgcgcagac ggctcactaa
     3901 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc ctctccgtta
     3961 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     4021 cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     4081 tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc ggcgtagcta
     4141 ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc tgcggtaacg
     4201 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca
     4261 aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt
     4321 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4381 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4441 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4501 gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt ggacacaaag
     4561 gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg cgggaattca
     4621 accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag gagcgcgact
     4681 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4741 acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg gctcgccatc
     4801 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4861 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4921 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4981 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     5041 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     5101 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     5161 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     5221 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     5281 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     5341 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5401 ccatccgcga atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5461 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5521 acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5581 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5641 cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa gctgggccgc
     5701 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5761 aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5821 attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5881 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5941 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     6001 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tgctgaaaac
     6061 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     6121 caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat actgcatgat
     6181 gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc tggatttagt
     6241 acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg
     6301 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6361 ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6421 gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6481 ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc
     6541 gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6601 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt caggttggta
     6661 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6721 ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6781 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6841 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6901 cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc cgacagctgc
     6961 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     7021 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     7081 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     7141 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     7201 tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     7261 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     7321 ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga acgccatcga
     7381 gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa
     7441 tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca tccagttctt
     7501 tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg aaagcaaccg
     7561 ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg
     7621 cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca acttcaacct
     7681 gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc cgttccgaac
     7741 aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc tattacagca
     7801 gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7861 aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7921 cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt
     7981 tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt ttgctgccgt
     8041 tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     8101 ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg cgatgaaatt
     8161 cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag cggcatgggg
     8221 tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat ccgtcaccgg
     8281 aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac gagttttcgc
     8341 cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa cattgcgatc
     8401 gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa tttgcaagct
     8461 ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa atgagcgatc
     8521 aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaatgtcga
     8581 taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa tcagatggaa
     8641 tt
//
LOCUS       DAJTUM010000144         8372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTUM010000144 DAJTUM010000000
VERSION     DAJTUM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO471_003698"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO471_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO471_003699"
     CDS             1127..1234
                     /locus_tag="OO471_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244812.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO471_003700"
     CDS             complement(1287..2045)
                     /locus_tag="OO471_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6244813.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO471_003701"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO471_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6244814.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO471_003702"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO471_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6244815.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO471_003703"
     CDS             3758..4618
                     /locus_tag="OO471_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6244816.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO471_003704"
     CDS             complement(4659..5195)
                     /locus_tag="OO471_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6244817.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO471_003705"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO471_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6244818.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO471_003706"
     CDS             6173..6763
                     /locus_tag="OO471_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6244819.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8286
                     /gene="tcyP"
                     /locus_tag="OO471_003707"
                     /pseudo
     CDS             6896..8286
                     /gene="tcyP"
                     /locus_tag="OO471_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2173 a         2004 c         2006 g         2189 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctagacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     8281 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     8341 atgttccatc gcggcgatgc atactgactc at
//
LOCUS       DAJTUM010000145         8334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTUM010000145 DAJTUM010000000
VERSION     DAJTUM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..159
                     /locus_tag="OO471_003708"
     CDS             <1..159
                     /locus_tag="OO471_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244820.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO471_003709"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO471_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6244821.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO471_003710"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO471_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6244822.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="OO471_003711"
     CDS             875..1246
                     /locus_tag="OO471_003711"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6244823.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFVVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="OO471_003712"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="OO471_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="OO471_003713"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="OO471_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6244824.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="OO471_003714"
     CDS             6949..7509
                     /locus_tag="OO471_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6244825.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="OO471_003715"
     CDS             7640..7852
                     /locus_tag="OO471_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6244826.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8334
                     /locus_tag="OO471_003716"
     CDS             8096..>8334
                     /locus_tag="OO471_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6244827.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1914 a         2084 c         2671 g         1665 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gttgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgtgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca cata
//
LOCUS       DAJTUM010000146         8118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTUM010000146 DAJTUM010000000
VERSION     DAJTUM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="OO471_003717"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="OO471_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO471_003718"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO471_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6244828.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO471_003719"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO471_003719"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6244829.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO471_003720"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO471_003720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6244830.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="OO471_003721"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="OO471_003721"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6244831.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7784..7859
                     /locus_tag="OO471_003722"
     tRNA            7784..7859
                     /locus_tag="OO471_003722"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7817..7819,aa:Gly,seq:gcc)
     gene            8017..8092
                     /locus_tag="OO471_003723"
     tRNA            8017..8092
                     /locus_tag="OO471_003723"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8050..8052,aa:Gly,seq:gcc)
BASE COUNT         1856 a         2093 c         2201 g         1968 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
     7801 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa
     7861 aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag
     7921 gcggcaactg aacgaatcct tgggaactta caaaagtatg tgactaaggt gagtgaaggc
     7981 agccaaggca gtagcggttc gaaagacgaa gattaagcgg gaatagctca gttggtagag
     8041 cacgaccttg ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttaa
     8101 aagtgctgta aggcatag
//
LOCUS       DAJTUM010000147         8079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTUM010000147 DAJTUM010000000
VERSION     DAJTUM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(99..674)
                     /locus_tag="OO471_003724"
                     /pseudo
     CDS             complement(99..674)
                     /locus_tag="OO471_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            678..1078
                     /locus_tag="OO471_003725"
                     /pseudo
     CDS             678..1078
                     /locus_tag="OO471_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1481..2062)
                     /locus_tag="OO471_003726"
     CDS             complement(1481..2062)
                     /locus_tag="OO471_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244832.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2109..5966)
                     /gene="sigA"
                     /locus_tag="OO471_003727"
     CDS             complement(2109..5966)
                     /gene="sigA"
                     /locus_tag="OO471_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6244833.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(6327..7331)
                     /locus_tag="OO471_003728"
                     /pseudo
     CDS             complement(6327..7331)
                     /locus_tag="OO471_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            7316..7693
                     /locus_tag="OO471_003729"
                     /pseudo
     CDS             7316..7693
                     /locus_tag="OO471_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7732..>8079)
                     /locus_tag="OO471_003730"
     CDS             complement(7732..>8079)
                     /locus_tag="OO471_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6244834.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1988 a         1882 c         1844 g         2365 t
ORIGIN      
        1 gctcagctcc acgccctgac gacggtatat ttctgactgg cggtataact gtcaacgacg
       61 gatgaaaagt ggctcttccg caacctgggt ggagattttc atgccagtgg actcattacc
      121 ctctgcctga gtagttcaaa tcctgctcga ccatacatct ggcgtttcag catcttcttc
      181 aggcgattta catgaccttc gacaacacca ttactccagc gactactgat tgcctcacat
      241 atagctgcag catcagcttc catccccgcc gccacgcgcc gaaattctgc tgaaccgctt
      301 tcgtggactc gagtgaacca actgcttagc tgtgatttat tttgggtttt cagaatacgg
      361 tagaactcga gtgccagttg ctgcgctatt ttcagctcag gttctttttc acacatcaga
      421 ctaataaatc gggaagcata attttcttca tccctggtta ttctccaggg catcaaccag
      481 cggcttaccc gggagactga aggaagtctg acggcagtag taaccggtgg gttccagcct
      541 ttacgccatt tggcaaccgc atccctgacg atggtttcac tgcctgtaaa tccccgggcc
      601 accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg ctcttttaac
      661 cactccctcc atggtgtcag cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt
      721 tgcggaaaag atgaagaccc agtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc
      781 acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa
      841 caacatcgct gaaaatgcct tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt
      901 cggctctgac catggtggtg agcggggagc gctactgtac agcctgatcg ggacgtgcaa
      961 actgaatgac gtggatccag aaagctacct tcgccatgtg cttggcgtca tagcagactg
     1021 gccggtcaac cgggtcagcg aactgcttcc gtggcgcata gcactgccag ctgaataaca
     1081 catccccgtc aatacggccc tcgctgtacg cttactcatc acctgctatt aatccccgag
     1141 tcagtacttc gcggcctgac gcgatcaccc tactcagtac ttcagagccc gcctccggta
     1201 cctgcctggc gatcgcccct gtgcaacaaa ataaatcaac cgttatcaaa ccacgatcgt
     1261 tcgtatgcgc cgccgatgac gcgcgcctga aaaacgcgtc aatatgagtt ccccggaagc
     1321 ttacgataat acgcttatga acattacaac acgctcatgc ggaacctgga tgggggaagg
     1381 aacggtgaag ctgttagcga atgaccggcg tttatacaaa cgccggtcat gatgaatgat
     1441 atttcagaat atacctcggg ctaaaatacc gggacagaga tcaggcgtga ttaatctgca
     1501 gcatttttaa taacagaagt actgagctta ctttaccttt gatgatgtaa tagttatttc
     1561 cgtcggtact catttttccc tgaaataatg tcacggatgt ttcttcctgc tgatcctgca
     1621 ggcgcagtcg taaggtcata tttttaatga gctgagatcc gggtggcaag gggtatttgc
     1681 tgtcagatac tgggcgactg agtctgatgt ggcgcgtata cagcccgttt tcgtacaggg
     1741 tggcctctgt tatggtgtca gtgaccagca tgtgggagac aatgctgtat tcttttttaa
     1801 agcacccacc agaaacggtg attgtaatga taatggtctt ctcgatcgag atgtaaatac
     1861 ccgtttcgtt atactgtccg tatcggaacc atgagaactc agttgcattc cagccatgct
     1921 ccagaatatg tgcatttttt tgcctgatag tttctttttt tattcgctca tgtttaaggc
     1981 ataacaggca gataaataat gtcagaaaaa gaaagctaat aatgagagca agagcgatat
     2041 agtctgtcac agggtaatac atattctctc cggtagaaga agggccgcaa acgcggcccg
     2101 ggctgttatc agaaagagta acggaagttg gcgttgatcg cgttatccac gttgtattta
     2161 ccaaatgccg atttctcaaa ctcaagaccg aagcgaagat tatcgcgaat ttcggcgttg
     2221 agaccaacat tcatgagaat acgaccgtct ttttcacctt tgatacgttt ctcaccggac
     2281 gcatcacgca gtacggtttc accgttggca aacaggtcaa actggtagcc aaggccggcg
     2341 cgggctgtga ctttccagtc cttaccggag aaggatttac ccacatcaac accggtacgc
     2401 ccaatcagcg gattaaagtc cttatccttc atggtcacgc tcattccgcg atcctgccag
     2461 tcgaaccgtt tacctgatac ggccccataa accagttctg cctgcggctc aatccaggag
     2521 tcttctgtta catgatagcg gtagcctgct tcagcaccca catacaagga atgagaactg
     2581 tagtcttttg ttccgagtcc agcaaaggtc gccgagtact cattatcatg gtgaacatac
     2641 ttactaatca ggtcgatata ggcaccagag tcaaatatag cggaagcata cagacctgcc
     2701 ccgacggact tcgtcttgcc actgaatgca ttactgctgg catgactgtc ggtatacgtc
     2761 atagtcagac cagtgaaaag gtcaagtcca tccagctcat gttttttatc cacaccaatc
     2821 tgcacatggg tgtagttgtc actgtatcca ccagaagctg aacctgctcc gctcatgatg
     2881 cgggcccatg caccggcctc gccattggtg tcacgcagat cccccattcg tttattcagg
     2941 ttgttgactt ctgtcaggaa ggctttgtaa ttaccggaca tatacgattt tgctgcaccg
     3001 gaagatgctg tattttcagc caccttatat ccggtcaggg tccacgtggt atgttcctga
     3061 tcctctttcg tgctgatcac aggagatatg ttgctgaaac ctatggcctg agtactggct
     3121 ttaaatatct ctttattgct tccttttggg gctgttatca ggtcaacgtt taatcctgag
     3181 ttattatttc ccactttctg tatatcgacc gacagactgt tattgcttcc ggttaactga
     3241 tttttaacaa tgagtttatc cgattgattt acagactggc tcagatcagc ccgcataatc
     3301 acagcagtat tagtggcatc cagtgtgttt actgtcacag tatggaaacc cgaattctct
     3361 atgttactaa acaacaaaag actgtcgcgg gtttttaatg attgcagggc tgactgtccg
     3421 tttactctcc ataaggtgtt atacatgctg gctgaggctg caggagcatc aatacctccg
     3481 gtataagaag tatcaaagcc acccagcaaa cctgtagcca gcggtgtgtg tgtggttttg
     3541 tccaggttat cttctttata ctggtcaaca ccaaacagaa tagatgcttt tttgtctgat
     3601 tttatattac cattgaccca ggcataattt tgagccttta acgtggcacc atcttctgtc
     3661 atattatatc catctgacat atagattgcc gggctgtatt tattattttc tgtaggggtg
     3721 cctgttaatg tcagggttcc ggccagttca atagctttat ctacatacat ttttccctgg
     3781 gcggtaacag tagctccttt gttgataatc aggtctgtat taattaagga taatgcacca
     3841 gtcatagtaa cagttgattt atcaccaaca gttattttgc tgttttgagc actcagactg
     3901 ccgttgaaac tactttcgcc tgctgaatcc ccggagcgaa gctgttgtct gaatccaaaa
     3961 ccttcgccag taatgtttct tcctgagaac atgtcaatgt atgcagtttt atttcccaga
     4021 acaatttgag agtttttagc ctctatgtgt ccttttacat tagcatcccg gcctatactc
     4081 agggttgcgt tttctaaatt taatttccgg aaattaaatt tacgactttc ccagtcgggc
     4141 tgggaaaaag aggctatctg attgttggac ttatattccg tattgtaacg tttatttaca
     4201 gtgctttcct ggtcctttat ggcagcagaa tagtcttttt gacataaaaa aggaattgga
     4261 cagttgttat tgccttcttt aaatatggca tgttctgtcg catgtccttg taatgttaac
     4321 tgtgcattcc ggacagagat atcgtttttg atatcgactg agccatcaaa gatcagattg
     4381 gcattattgt tatgttgctg ggtagtattg ataatatgat ttatgcttat attacctgat
     4441 acattcccat ggtacatata tgcatcagta ttagtaagtg ataatgttga ttgcttcacg
     4501 tttgagttcg ttacggttgt tcccgcatct gttgctgcga ttttctgaaa tgactggtca
     4561 tagccgttta gatccagtgt tccgccattt ctggtaaaat agatttcgcc attaccgctt
     4621 tcgcttaaag cgtctttggc atttattttt accgtacctt tacctccggc catgtaaatt
     4681 ttattgaagg ctttttcagc actaaggatg acagtcccat tacctatttt aaggttattt
     4741 ccctgtgcta tttttacatc cagagtacca ctacctattt tatgcagatt atctccggca
     4801 acgcccttaa cattccattc aacggtggta ttattatcaa tatctatccc ggcaccttta
     4861 taagacttat cttttccgta cactttatat gtctggttct tatcgaatat aagcccgcca
     4921 atgccaagat cggtatcctg cttgagcgtt aatacccctc ctcctgagac agacaggtcc
     4981 ttatctttta taatcgatga taactcttgt tttttaccat ccagctgcat agtgtttgtg
     5041 aaattctttt ttaaattgtt taccagaata ttattaaata acgagtagtt tgtccaggct
     5101 atattggtac tactgataaa accaactgta ctggtaacac catggaaaac ccatttttgt
     5161 tgttctttat catatagata agaaccagaa ccactatcgc caaaaattgg tgagttttta
     5221 aaaggggttc tgttggtata aatgtaactc ttgtttttcc agtccagatt aaaaagactt
     5281 gcgcttaaca agtcgggatt atagttttgt ccaaacagat attgtttgcc gtttaatatc
     5341 gttgaggttg ttccggcacc tgctctgaag ccaattattt gttccttacc gttgtagttt
     5401 acgccatatc ttttcatcgc atcttcggga gaaagtgaga agtcggcact ctccgtaacc
     5461 cctgttgttt ccacgacgaa tttattcaga cgatgaaccg aaaagtcgcc agatgatact
     5521 ctgttacttg ctttatatgc tgtctgatcc catgactgtg ttgttattgc atgatgctgt
     5581 gtaccgttat gagtcgcagt aacactgtac gcacctccaa ttgatgttgt tgcacctttg
     5641 ttggaaacag cagaaaaatc aggtacaggt agttctggaa aatgaaatat ctttccatct
     5701 ttcatcatta attgaacatt tgttgcaccc ggtctgaact ctcctctgtt ttgagcaagg
     5761 tcaagataat cgcgggtcca tatattattt atatccagca ttgctgcaaa tgctgtttcg
     5821 ccataacttc catatattga ggataaaata ataccgagga taactttctt tcttgatgta
     5881 ccgacactaa ctttccgggt cagttcagaa acagcaacta atccacctgt aatatgacta
     5941 tatttcagtg aataaatttt attcatacgt gaactccgat atttctgagt tgtggttatg
     6001 ggatagcatt taattacaac ggggaactat cagtcatggt acgtaactga taagggtata
     6061 atgataatca ctgtcagtta tatttgtatg acagatgtaa caaaatgaaa aaaaagataa
     6121 gcacaagtga ttgattggtt agatattgat cacttggtgt ttgtattttg aattttgcga
     6181 tcaaaatttg atctttgttg tttccggtca atcctttaca ggttttctgt attatccggg
     6241 ggagttaagt ctgtcgatgt ccgacagact tttgatgatg gaaacaacat gcagcgataa
     6301 tgtgcatgct gttttgcagg tggaactcag aaggtgaact ggaactgtgc gaccgcaccg
     6361 taagtgttgt ccgtgccgat caggcctgca acatcaaggt gaaccacatc aaacggtgag
     6421 atccccaccc cggccgtgaa tgcactgccg tcgttaccgg ccagattctg acggtaaccg
     6481 gcgcgcagct gtgcccattt ccaggcatta aattctgtgc caatggcggc aaactgacgg
     6541 ttgctgtcgg aggtgaaacc gctggccggg gtcagatcca catcaaatgc agtggtgaac
     6601 atcgcgttgt gccaggagac accggccgtc acctgcgggc ggatcctgaa ggtttccgtg
     6661 ataccgttca cttcttttgt ctcaatacta cgggagatca ggttctgtgc gaccagtccc
     6721 agcgtccagt tgtcatccag gtccgtactg gcaccgatgt cggcgttgat gccatttttc
     6781 gtgttgtggt agcggtcgcc tttgaaggcg ctgctgtcat aatttctgac cagcacgttg
     6841 tagttgaaca ggtcgacgcg ctggaatttg ggggggaagc ccagagacca tttctgcccg
     6901 gccgtttcca gttccgtagc cagagcaata cccacatcag tgatcagtgc cgtgcgggca
     6961 caggcctgtg atgtcaggcg gcttttgtcc acctcctgcc cggtgctgtt tgccacctct
     7021 tccaggtaag tgaggtcagc atcagagact ttaccgtcga cactgacggt gccgtgtgat
     7081 ttaaccatca gtgcggcaga gaggcgatca ccgggaaggg ccgccacagc agaaacgccc
     7141 agctgtgcgg cggcatgtgt gttacggaag tcccggaggt gttctttcag gcgtgcggca
     7201 gcctcaggaa caccgtggtt actttcgatg gcgcggtcga aggccttcca ctcatcactg
     7261 atccggtcgg cgttgtcggt gatattatcc ggattggaga gctgcgcgcc gacggacgga
     7321 agcaggatgc tgggctcatg cgcgacgcaa aatccacgat gtatatatca gtactaaaag
     7381 cgcgacggcg gaagaagctc tgaaactaat cggtgaactg tacgccatag agcacgatat
     7441 acgcgggtta ccggtgtctg aacgcctggc ggtcaggcaa atgcagagta gaccgctcct
     7501 gacttccctg tataagctga tgcaggagaa agaacacacg ttatcgaaga aatgtcgtct
     7561 gggagatgcg ttccggtata tcaggaagca ctgggctgcg ttgtgcaact tctgtgatga
     7621 tggcctggct gaggtagata ataatgcagc agaaagagcg cttcgtgcag tctgtctcgg
     7681 gaagaaaact ttttcgagga tcactcattc cgtgtctgac tgcttcaggg tttaattttg
     7741 cttctgctga ctgaatgatg attgggcagc aagtgcaata tcccacataa atccagtcaa
     7801 ctcacgagcc accgccgtaa tggctacttg tgattttttc ccttttgctg tcagagttcg
     7861 gtaacgagaa cacaatcgtt gttgagcttt ccatgcaata tctttggaat attgtgttac
     7921 agagtccggt cggtaatata taagccatgc tccaactttt gcaggtgtac gataagacca
     7981 ggcggcttcg taaagcagac gtctcagttc gctgtttcca acttttgtta ttcctctggg
     8041 acgaatactg tttccactgg aatattcacc tggcaccag
//
LOCUS       DAJTUM010000148         8065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTUM010000148 DAJTUM010000000
VERSION     DAJTUM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            7..657
                     /locus_tag="OO471_003731"
     CDS             7..657
                     /locus_tag="OO471_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244835.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO471_003732"
     CDS             629..1063
                     /locus_tag="OO471_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6244836.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO471_003733"
     CDS             complement(1836..2885)
                     /locus_tag="OO471_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6244837.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINAINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO471_003734"
     CDS             3336..4526
                     /locus_tag="OO471_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6244838.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO471_003735"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO471_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6244839.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OO471_003736"
     CDS             complement(6373..7302)
                     /locus_tag="OO471_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6244840.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OO471_003737"
     CDS             complement(7299..7661)
                     /locus_tag="OO471_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6244841.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2492 a         1533 c         1634 g         2406 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgcgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta
     7861 taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaata
     7921 agttacctcg catttaagta aactacgttc tcctcttgtg ccgtatttgt gccattgcga
     7981 cttataatcg catcgatttt gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg
     8041 tatctttgaa ccatttcgag agttt
//
LOCUS       DAJTUM010000149         8027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTUM010000149 DAJTUM010000000
VERSION     DAJTUM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..1779
                     /locus_tag="OO471_003738"
     CDS             <1..1779
                     /locus_tag="OO471_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6244842.1"
                     /translation="SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLG
                     VIGYFIEHKSRNTLLFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLT
                     LKRFSSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGS
                     VWPKSIRGSTPKIKGSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKW
                     EKGKPETVYYLCEHNGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPR
                     SITFHIWTAYSPFTTRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVL
                     MDKVVPYTATVPVRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEE
                     TLLRVDAAINKKYRHADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASV
                     YGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEI
                     FSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDL
                     AVQLHPARMQLHNRPLKNWQRSCPEE"
     gene            1780..1983
                     /locus_tag="OO471_003739"
     CDS             1780..1983
                     /locus_tag="OO471_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6244843.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            1980..3573
                     /locus_tag="OO471_003740"
                     /pseudo
     CDS             1980..3573
                     /locus_tag="OO471_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3563..5068
                     /locus_tag="OO471_003741"
     CDS             3563..5068
                     /locus_tag="OO471_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6244844.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5105..5452
                     /locus_tag="OO471_003742"
     CDS             5105..5452
                     /locus_tag="OO471_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6244845.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5510..6538
                     /locus_tag="OO471_003743"
     CDS             5510..6538
                     /locus_tag="OO471_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6244846.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6590..6976
                     /locus_tag="OO471_003744"
     CDS             6590..6976
                     /locus_tag="OO471_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6244847.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            6988..7365
                     /locus_tag="OO471_003745"
     CDS             6988..7365
                     /locus_tag="OO471_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6244848.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7352..7936
                     /locus_tag="OO471_003746"
     CDS             7352..7936
                     /locus_tag="OO471_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6244849.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            7933..>8027
                     /locus_tag="OO471_003747"
     CDS             7933..>8027
                     /locus_tag="OO471_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6244850.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPA"
BASE COUNT         1847 a         1899 c         2501 g         1780 t
ORIGIN      
        1 tcatacggtg agggggaatg gaaaacgctg cagtttcagg tcgccatcat gaactgcatg
       61 ggcaatgacg agattcgtac agttaacctg attaaatctg cccgtgttgg ttatacaaaa
      121 atggtgcttg gtgtgatcgg gtattttatt gagcacaaat cccgcaacac tcttcttttt
      181 cagccgacgg actcagctgc tgaagatttt atgaagtctc acgtggaagc aacaattcgt
      241 gatgttcctt gcctgaaaaa actttctccc tggctgggcc ggaaacaccg ggataatacg
      301 ctcacactga agcgtttttc ctctggtgtg gggttctggt gtttgggtgg tgctgctgcc
      361 aaaaactacc gtgaaaaatc tgtcgatgtg gtctgctatg acgaactctc ctcgtttgaa
      421 ccggatgtgg aaaaagaagg ttcgccaaca ctgcttggcg ataaacgtat tgaaggttcg
      481 gtatggccaa aatccatacg cggttcaacg ccaaaaatta aaggctcctg tcagattgaa
      541 aaagctgcga atgaatctgc gcatttcatg cggttttatg tcccttgccc tcattgcggg
      601 gaggagcagt atctgaagtt tggcgatgat gcgacgtcgt ttggcctgaa atgggagaag
      661 ggtaaaccgg aaacggtgta ttacctgtgt gaacataatg gctgcgtgat ccgtcagtct
      721 gaacttgatc agaccggggg gcgctggatt tgtgacaata ccgggatgtg gacgtgtgac
      781 ggtctgacgt ttttcagtgc ttcgggtaat gaaattccgc cgccgcgctc catcactttc
      841 catatatgga cggcgtacag tccgttcacc accagggtac agatagtctg tgactggctg
      901 gatgcactga aagatcccaa cggcgtgaaa acttttgtga acaccacgct gggcgagacc
      961 tgggaagagg ccgtgggcga aaaactcgat caccaggtac tgatggataa ggttgtgcct
     1021 tacacggcaa cggtgcctgt ccgggtggtt tatctgacgg cgggcattga ctcgcagcga
     1081 aaccgttttg agatgtatgt ctgggggtgg gctccgggag aggaagcctt tctggtggat
     1141 aaaatcatca tcatgggacg tcctgatgag gaagagacgc tgttacgtgt ggatgcggcg
     1201 atcaataaaa aataccgcca tgcagacggt accgaaatga ccatttcccg tgtctgctgg
     1261 gatatcgggg ggatcgatgg cgaaatcgtg tatcagaggt caaaaaaaca cggtattttc
     1321 cgggtactgc cggtaaaagg tgcctctgtc tatggcaagc cggtgatcac catgccgaaa
     1381 acccgcaatc agcggggtgt gtatctgtgt gaggtgggga cggataccgc aaaagaaatt
     1441 ctctatgccc gaatgaaggc cgatcccacc cctgtgaatg aggccacatc gtatgccatc
     1501 cgctttcctg ataatccgga gattttttcg cagacagagg cacaacaact ggtggcggag
     1561 gaactggtgg aaaagtggga aaaaggaaag atgcgtctgt tgtgggataa caaaaagcgg
     1621 cgtaacgaag cgctggactg cctggtgtat gcctatgcgg cattacgtgt gtccgtgcaa
     1681 cgctggcaac ttgatctggc tgtgcagttg catcccgcaa ggatgcaact gcacaaccga
     1741 cccttgaaga actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg
     1801 caggcgctgc gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag
     1861 aaagacggtc gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc
     1921 aacgatgcgg agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta
     1981 tgaaacgaac gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct
     2041 acaacggagg cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga
     2101 gtgccgatgc ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg
     2161 tgcgcaataa cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc
     2221 acatgtttct tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg
     2281 caaaaagttt tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg
     2341 gcgagatcga cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg
     2401 tgcatgcctt taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt
     2461 tattccgtac gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta
     2521 tggggaacca ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt
     2581 accatatctg tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc
     2641 gtgagcttcc caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc
     2701 agacccgtgg ggctaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc
     2761 tgcaggccac gcagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa
     2821 gtgaacttga taccgaaaag gcatttgaat atatcgcggg ggcaccgcaa ggtcagcagg
     2881 ataatccgct tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga
     2941 cgctgggcgg tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca
     3001 gcgcaggatt cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca
     3061 gccggtcttg gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca
     3121 agtgcccgcg cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt
     3181 gcggcccggc tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg
     3241 attattcgtc cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg
     3301 gcagagtgga ttagctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg
     3361 gtgatgcgca ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa
     3421 gattatcagg acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga
     3481 ctctcacgtc cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg
     3541 ccggaagagg agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt
     3601 caatgagccg ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg
     3661 tgagatgggc gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg
     3721 gatgctggct gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg
     3781 ggtggtgaac ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg
     3841 catgcggcca ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc
     3901 aatggcggac actcaggtac ggggcgtact gctggacatt gacagttctg gcgggcaggc
     3961 caccggcgcg tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg
     4021 ggcactgtgc aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg
     4081 acggctggtt acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag
     4141 ctatgccggt catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca
     4201 caaggtggat ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca
     4261 gcgcattgat gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc
     4321 tgtggatgcg gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc
     4381 cgaactggcg gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct
     4441 gaacacacac gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca
     4501 ggagaaccag cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact
     4561 ggccacgatg ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc
     4621 cgtggcggca ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc
     4681 atgtgaagag gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat
     4741 gacggtggaa aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc
     4801 acttcgtgat cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa
     4861 actggcggcc tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc
     4921 aggcagggca gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa
     4981 ccattcaccg gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga
     5041 cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta
     5101 caatatggta acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa
     5161 tgatccggcc cataccgcca caggctgcag cgggatttcc tcggcaacac cggctctgac
     5221 gcccctgatg ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg
     5281 ccatgtggtt ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta
     5341 caagtcgggg acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa
     5401 aaaggcgaat gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat
     5461 caggtcactg cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt
     5521 tacgacccgc cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct
     5581 ggagctgttt ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat
     5641 taccggaaaa acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg
     5701 tcaccgtggc ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt
     5761 taattaccag caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacggccc
     5821 ggcttaccgt cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt
     5881 ccaggtggaa gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg
     5941 agagcagttt gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca
     6001 ggcaggaggg acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct
     6061 ggatgcgtac tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt
     6121 ctggcgtctg ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc
     6181 aaattcacag ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta
     6241 ttatggtgat ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga
     6301 aaaacgttat ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg
     6361 ttgctacggt gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg
     6421 ttatccgaaa cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc
     6481 cgcgccgctg atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc
     6541 cgtgagcggg agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa
     6601 agaagaaaac ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt
     6661 ctccggtagt gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga
     6721 tgcgggggag gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca
     6781 taaaggcgag tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt
     6841 tacggtggtg gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc
     6901 accgggaacg tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct
     6961 ggcaaaaagg cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg
     7021 cttgccggtg tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc
     7081 ggagaacttg aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt
     7141 gttgccgggg ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat
     7201 atatcgcagt tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat
     7261 cgtattactc cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc
     7321 ccacagaata atgccagaat gaggcgatat gatgaaggga cttgagaatg ccatccgcaa
     7381 tctgaacagc ctcgataccc gcatggtgcc acagaccagc gcatgggcgg taaaccgtgt
     7441 ggcggcaaag attgtttctg tcgccacacg gcaggttgcg cagaataccg ttgccgggga
     7501 taaccaggtg aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc tgcttaaagc
     7561 cagcccctca ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc tgcccgccat
     7621 taagctgggt accgcacagg tcagactggc ccgctcccgg catggcagca actcacgtca
     7681 tcgtggcagc gtactgaaag tggggaaata ccttttccgg gatgcgttta ttcagcaact
     7741 ggcgaatggt cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt accccattga
     7801 tgtggtgaaa atcccgatgt ccggaccgct gacacaggca tttgaagatg cccgcgaccg
     7861 catcattgct gcggaaatgc cgaaacagct ggggtatgcc ctgaaacaac aactgaggtt
     7921 acatctgagt aaatgaaccg acacacacaa atccgtcaga gcgtgctggc acgccttcgg
     7981 gaacagtgtg gagacagcgc cacgtttttt gacgggcttc cggcatt
//
LOCUS       DAJTUM010000150         7949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTUM010000150 DAJTUM010000000
VERSION     DAJTUM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..176)
                     /locus_tag="OO471_003748"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="OO471_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            174..498
                     /locus_tag="OO471_003749"
                     /pseudo
     CDS             174..498
                     /locus_tag="OO471_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(528..3440)
                     /locus_tag="OO471_003750"
     CDS             complement(528..3440)
                     /locus_tag="OO471_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244851.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3443..3559
                     /locus_tag="OO471_003751"
     CDS             3443..3559
                     /locus_tag="OO471_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6244852.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3923..4765
                     /locus_tag="OO471_003752"
     CDS             3923..4765
                     /locus_tag="OO471_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6244853.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4768..5856
                     /gene="capU"
                     /locus_tag="OO471_003753"
     CDS             4768..5856
                     /gene="capU"
                     /locus_tag="OO471_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6244854.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5861..6811
                     /gene="virK"
                     /locus_tag="OO471_003754"
     CDS             5861..6811
                     /gene="virK"
                     /locus_tag="OO471_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6244855.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6876..7820
                     /gene="lpxM"
                     /locus_tag="OO471_003755"
                     /gene_synonym="msbB"
     CDS             6876..7820
                     /gene="lpxM"
                     /locus_tag="OO471_003755"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6244856.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2139 a         1777 c         1886 g         2147 t
ORIGIN      
        1 ttttttacca ccgggagttt gggcataacg gtgctttcag taagtacgac aggtactgtg
       61 gaggcacttc cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc
      121 catgaaagcc ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc
      181 agcggcggac agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt
      241 acccggtcaa aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt
      301 taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag
      361 gcctcccgga agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat
      421 ctgctggagc aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg
      481 tacttacgta cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga
      541 gcggcacgac ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa
      601 cccttcttcc ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc
      661 aaatcatgcc ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc
      721 ttccttattt taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc
      781 ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa
      841 ctccttcgtt tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac acactctgcc
      901 agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac
      961 agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg
     1021 cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt
     1081 ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc
     1141 agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt
     1201 gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga
     1261 tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc
     1321 cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta
     1381 aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc
     1441 agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt
     1501 cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta
     1561 cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc
     1621 cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc
     1681 gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac attgtgttta
     1741 gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga
     1801 tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga
     1861 ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc
     1921 gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag
     1981 catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt
     2041 gaggttttgg cattcagttc ctgctttgtc tcttccctat cgtccagcaa ccggatggtc
     2101 ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag
     2161 cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg
     2221 taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga
     2281 cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag
     2341 agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc
     2401 atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac
     2461 tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt
     2521 tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc
     2581 agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg
     2641 cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg
     2701 ggcagccagt cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt
     2761 tccagaaatt ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa
     2821 gcgtcataga gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata
     2881 tcaccagcag cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag
     2941 ccaacaatca ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac
     3001 agcattttat ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca
     3061 gtcgcgatat caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc
     3121 acgtttctgc gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca
     3181 aggcagatag tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg
     3241 agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg
     3301 gtggcaatag cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga
     3361 taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga
     3421 aggaatgcct tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca
     3481 gaaaaatgct ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc
     3541 gctggaaatg gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca
     3601 ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg
     3661 agtacctgtg ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt
     3721 gccacctcaa ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat
     3781 gaattgttga tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat
     3841 tcatccagta tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct
     3901 tgctaggtgc cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca
     3961 tgttccggta ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc
     4021 cgtgacattc cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc
     4081 agatgaactg gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac
     4141 atttgatgat ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa
     4201 tctcaaagca catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc
     4261 tcctggaaag gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga
     4321 taatgttatg cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt
     4381 tcatgttcat acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca
     4441 gtgtaaacat cttcggcagg acttgttgag cgggagggaa tatttgaaag aaatgacagg
     4501 aaaatgcagt aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt
     4561 cgctgaagaa cttggattct attatttata tacaacagaa agaagaatga atgctcctgc
     4621 taaaggaact acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct
     4681 gaaaagacga cttttttatt atacaacccc gtttttttca tcgttgctgg cctttcataa
     4741 ggggccaaga ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat
     4801 ataggtggtc aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat
     4861 tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattgggg
     4921 attgatatca cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta
     4981 ctcggaatag ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc
     5041 aatattgttg gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa
     5101 aagacatatt tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg
     5161 attgttcccg gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt
     5221 actgttgtgc ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg
     5281 cctccaaatg ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta
     5341 ggaatgttgc gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa
     5401 atgaatggac gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag
     5461 catttacagt atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat
     5521 gtttttcctg ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac
     5581 gaatcgtttg gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt
     5641 cagattggtg gaatccctga tgttattcag aataaccaga ccgggacatt gttaccagca
     5701 ggtaataagc acgcatggat gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt
     5761 tatcagatgg ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact
     5821 gcgttaaaaa tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt
     5881 atcatttatc ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg
     5941 ggaacacaga aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag
     6001 tattcgatat tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca
     6061 acccacgttg ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc
     6121 ccgggggcaa tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc
     6181 tgaatttttg tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga
     6241 ggattttgat atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc
     6301 attgtttttc aataaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa
     6361 tggacacacc gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatat
     6421 tattcggtgc gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc
     6481 attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag
     6541 tcatgttttc cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta
     6601 cagtgatttc tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac
     6661 acaggtggtt cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa
     6721 acgatacgct ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc
     6781 tgtaaactca gaacatcagg acttaaatta aggtttgatg tattctctga ataaaatatt
     6841 aatgatgatt ttggtagggg cattcgcact aaataatgaa aaaatacaaa tccgagttta
     6901 ttcctgaatt taagaaaaat tatctttccc ctgtttactg gtttacatgg ttcgttttgg
     6961 gaatgattgc aggtatttca atgtttcccc cttcattcag agatcctgtc ttggccaaaa
     7021 tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg attaatttat
     7081 cgctttgttt cccggaaaag agtgatacag aacgggaaat aattgtcgac aatatgtttg
     7141 ccacagcatt gcaatccata gtgatgatgg cagaactggc aattcgtggt ccggaaaagt
     7201 tccagaaacg agtgttctgg aaaggcctcg aaattcttga ggaaattcgg cacaataaca
     7261 gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg
     7321 cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt
     7381 atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt acattcccgg gaggatggga
     7441 taaaaccatt tattcagtca gtacgccagg gatactgggg gtattacctt ccagatcagg
     7501 atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac
     7561 caattattgg acgtctgatg aacatcagtc aggctatgat tataccgctt ttcccggttt
     7621 atgatgaaaa aaaacatttc ctgactattg aagttcggcc accaatggat gcatgcattg
     7681 ccagcgcgga caataaaatg attgcccgac aaatgaacaa aacagtggaa attttggtgg
     7741 ggtcacatcc ggaacagtat atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg
     7801 aagcggaccc gtacccttga aattataaag tacaggtatt tccactagtt gtttcttaca
     7861 ggttaccaat cgaaacacat cccccttccg tgtgttgccc gtttggcacg gaggactgtg
     7921 tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DAJTUM010000151         7742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTUM010000151 DAJTUM010000000
VERSION     DAJTUM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..1014
                     /gene="ydfJ"
                     /locus_tag="OO471_003756"
     CDS             <1..1014
                     /gene="ydfJ"
                     /locus_tag="OO471_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6244857.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1103..2563
                     /locus_tag="OO471_003757"
     CDS             1103..2563
                     /locus_tag="OO471_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6244858.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OO471_003758"
     CDS             complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OO471_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6244859.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OO471_003759"
     CDS             complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OO471_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6244860.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OO471_003760"
     CDS             complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OO471_003760"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6244861.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4638..6683
                     /gene="dcp"
                     /locus_tag="OO471_003761"
     CDS             4638..6683
                     /gene="dcp"
                     /locus_tag="OO471_003761"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6244862.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6728..7246)
                     /locus_tag="OO471_003762"
     CDS             complement(6728..7246)
                     /locus_tag="OO471_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6244863.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7522..>7742
                     /gene="ydeI"
                     /locus_tag="OO471_003763"
     CDS             7522..>7742
                     /gene="ydeI"
                     /locus_tag="OO471_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6244864.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISH"
BASE COUNT         1940 a         1812 c         1955 g         2035 t
ORIGIN      
        1 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
       61 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      121 gtggctatgg gaactaactg cggcacctta agcgcaacgg caatctgggc ctttatgttc
      181 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctggcgagc
      241 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
      301 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatgttc
      361 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcggg taactccggt
      421 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
      481 ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc gttccttggt
      541 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctctgcgatt
      601 gtgctggcgt ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
      661 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
      721 attaccatgg cagaaatgtt cggctgtaaa aaccgcttca cccggatggc tatttcaaaa
      781 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
      841 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
      901 atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct
      961 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaaccctc
     1021 aatcccttct cttaccggag aggggatttt tattcatata acaaaacata tagcttgcca
     1081 tatttaaatt taaggaattg ttatgggaaa taatttgtta tcagcaaaag cgacactccc
     1141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
     1201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca gtgactgggg
     1261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
     1321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
     1381 cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg ctgcgatgtg
     1441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
     1501 ggcgaccgga cagttaatgc tcgagcaccc gatggtcgct gccgacgtgc aaaatcccca
     1561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
     1621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
     1681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
     1741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
     1801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
     1861 tttccgccag tgggttatcg aagataactt tgtagccgga cgtccggaat gggaaaaagc
     1921 gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
     1981 cggcagtcat tcattcctgg catatctggg ttatcttgct ggatatcagc acattaatga
     2041 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
     2101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
     2161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
     2221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
     2281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
     2341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
     2401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
     2461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
     2521 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
     2581 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
     2641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
     2701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
     2761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
     2821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
     2881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
     2941 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtc
     3001 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     3061 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     3121 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     3181 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     3241 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     3301 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     3361 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     3421 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     3481 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     3541 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     3601 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     3661 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     3721 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     3781 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     3841 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     3901 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     3961 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     4021 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     4081 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     4141 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     4201 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     4261 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     4321 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctg
     4381 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     4441 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     4501 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     4561 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     4621 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     4681 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     4741 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     4801 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     4861 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     4921 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     4981 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     5041 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     5101 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     5161 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     5221 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     5281 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     5341 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     5401 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     5461 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     5521 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     5581 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     5641 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     5701 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     5761 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     5821 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     5881 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     5941 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     6001 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     6061 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     6121 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     6181 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     6241 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     6301 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     6361 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     6421 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     6481 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     6541 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     6601 atctggaacg cctgcatcga caatggcgtg gtaaggctcc tcagattatg ccgatgctgc
     6661 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     6721 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     6781 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg cctttaatat
     6841 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     6901 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcacct gttgccattt
     6961 ctgctgccac tttctcactc accgcagaat atcgttcaag agactgctgg cttacgctga
     7021 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     7081 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     7141 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg ttttcaatag
     7201 tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactgtt ttttgtccac
     7261 gctattatat gtaaaggtga tttgcttttt ctgtatgctc gtccaatata ataattttca
     7321 aacatataaa tccgattatt ctgaaaacat gtttaagact cttcaatctt cttaacttta
     7381 gtgagtcaaa ttctctgaat atttgacagc tagtgggctg attttaaatg ccttataaca
     7441 attggcagta ttcttatttg gcaattataa aaatagcaac cagacttaat aagtcaaacc
     7501 aaacgcaatt aaggagttag tatgaaattt cagtccattg tattagcaag ttttcttgtc
     7561 atgccttatg cgttggcaga tgaccagggc ggtttaaaac aggatgcagc gccaccgccg
     7621 cctcatgcaa tagaagatgg ctatcgcgga accgatgatg caaaaaaaat gaccgttgat
     7681 ttcgcaaaaa acatgcacga tggcgcttcg gtttcactac gcggtaattt gatttctcac
     7741 aa
//
LOCUS       DAJTUM010000152         7721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTUM010000152 DAJTUM010000000
VERSION     DAJTUM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..830)
                     /locus_tag="OO471_003764"
     CDS             complement(<1..830)
                     /locus_tag="OO471_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6244865.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="OO471_003765"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="OO471_003765"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6244866.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="OO471_003766"
     CDS             complement(2479..2706)
                     /locus_tag="OO471_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6244867.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="OO471_003767"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="OO471_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6244868.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="OO471_003768"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="OO471_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6244869.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="OO471_003769"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="OO471_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6244870.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="OO471_003770"
     CDS             complement(5528..6511)
                     /locus_tag="OO471_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6244871.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="OO471_003771"
     CDS             complement(6557..7381)
                     /locus_tag="OO471_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6244872.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="OO471_003772"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="OO471_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6244873.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccagcgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtat cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcactc a
//
LOCUS       DAJTUM010000153         7705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTUM010000153 DAJTUM010000000
VERSION     DAJTUM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..216
                     /locus_tag="OO471_003773"
     CDS             <1..216
                     /locus_tag="OO471_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6244874.1"
                     /translation="LRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWL
                     REVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            225..944
                     /locus_tag="OO471_003774"
                     /pseudo
     CDS             225..944
                     /locus_tag="OO471_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1283..2053)
                     /locus_tag="OO471_003775"
     CDS             complement(1283..2053)
                     /locus_tag="OO471_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244875.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(2098..2532)
                     /locus_tag="OO471_003776"
                     /pseudo
     CDS             complement(2098..2532)
                     /locus_tag="OO471_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2544..2765)
                     /locus_tag="OO471_003777"
     CDS             complement(2544..2765)
                     /locus_tag="OO471_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244876.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2766..3449)
                     /locus_tag="OO471_003778"
     CDS             complement(2766..3449)
                     /locus_tag="OO471_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6244877.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3513..3824)
                     /locus_tag="OO471_003779"
     CDS             complement(3513..3824)
                     /locus_tag="OO471_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244878.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3821..4723)
                     /locus_tag="OO471_003780"
     CDS             complement(3821..4723)
                     /locus_tag="OO471_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6244879.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            5163..6122
                     /locus_tag="OO471_003781"
     CDS             5163..6122
                     /locus_tag="OO471_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6244880.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6122..6517
                     /locus_tag="OO471_003782"
     CDS             6122..6517
                     /locus_tag="OO471_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6244881.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6700..7080
                     /locus_tag="OO471_003783"
     CDS             6700..7080
                     /locus_tag="OO471_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6244882.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            7126..7705
                     /locus_tag="OO471_003784"
                     /pseudo
     CDS             7126..7705
                     /locus_tag="OO471_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2086 a         1987 c         1891 g         1741 t
ORIGIN      
        1 ttacgatcgg tggcggttgg aagaaaaaat tatctctttt tcggctcaga caagggagga
       61 gaaagtgcgg cgatcatcta cagtctgctg gtcacctgca aacagaacga agtggagccg
      121 gaggactggt tgcgcgaagt gatcgagaag ctcaatgact ggccgtcgaa ccaagtgcat
      181 gaactgctgc cctggaactt ctcgtctgta aaataatcct tacgctacgt acttctcggg
      241 gcgcttacgt tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg
      301 tccagccaag aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc
      361 tcatcagaga taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa
      421 tcagcctgcc gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg
      481 aaatccccga atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg
      541 ttgagcaaat agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg
      601 ctcagcgcat tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc
      661 aacttggtga cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg
      721 ttccccggca gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg
      781 ataaaaattt acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac
      841 gccagcatgg aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg
      901 tggttgcctg cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc
      961 aaaaagaata tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac
     1021 aataagatac aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca
     1081 aatcgacctg attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt
     1141 aggattatca ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg
     1201 atagatttaa gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca
     1261 tagattttcc ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc
     1321 ggatttcggc ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc
     1381 ggggcggttc atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct
     1441 caatacaatc gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga
     1501 tggcatcgag caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc
     1561 tttccttcac gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg
     1621 tggtcagcgc cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata
     1681 ctgcatcgcg caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg
     1741 catacgggaa agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac
     1801 tgccggacag aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt
     1861 gcggcggcag cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca
     1921 gccgcacgta aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct
     1981 cttccggcca gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca
     2041 gcgtttcatt cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca
     2101 gtcgttttcg tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg
     2161 gcgggtaacg tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca
     2221 cagtgcctgt tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg
     2281 gcggttttcg cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc
     2341 aatccgctag tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc
     2401 ggacagggaa atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac
     2461 cagcatcagc ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca
     2521 tgttccgtac atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg
     2581 gtatatgcct ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc
     2641 ggcagggtgt cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg
     2701 tcgcacatgg cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag
     2761 ttcatttacg cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc
     2821 cgctgtacgg cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata
     2881 ccgatatacc ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac
     2941 gggtccagca ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta
     3001 acgggctttt ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc
     3061 agcgggtttt gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga
     3121 tagcccacat atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg
     3181 ctgaatcccg catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag
     3241 ctcaccatca gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc
     3301 cattcatcag ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga
     3361 tatggcggat cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg
     3421 atgcagttac cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct
     3481 gtggtgcccg ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg
     3541 accgtggaat accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt
     3601 gaagggcgtt catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg
     3661 gagcggtaaa agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt
     3721 ttatgccgcg ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg
     3781 gaaaagaaac gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca
     3841 cattgtcgac tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg
     3901 aagaccattc aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc
     3961 agccacagaa atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc
     4021 ggcttaattc tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct
     4081 gaatgaagtt ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc
     4141 cataccgttc cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa
     4201 agccgttcac ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg
     4261 ttttttccgg cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac
     4321 cccatatgtc acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg
     4381 catcatccgg taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat
     4441 gcagcatcag gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc
     4501 gggtgaacgc gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc
     4561 actgttcaga gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca
     4621 gctgaatacc ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc
     4681 gtttaatctc agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt
     4741 ttggcatggg aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc
     4801 gggcgaccgc atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga
     4861 cgaaacgtga ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg
     4921 gttaacgtgc cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga
     4981 gaaaccaata agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca
     5041 acaccgtttg acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag
     5101 atgcaccgac ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa
     5161 gaatgctaaa agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag
     5221 atggcgaggt aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac
     5281 tgtttaatgc cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt
     5341 tgggaagcac tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc
     5401 tgcttgccat acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga
     5461 cagtaacatt acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa
     5521 tagaaagaaa aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta
     5581 aaattaaaaa agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa
     5641 aagataaagt aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg
     5701 attgtggttt aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa
     5761 tcgggacatc ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat
     5821 gtaattattt tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga
     5881 cattaataaa tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga
     5941 aatctctggc agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct
     6001 atctcactgg cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc
     6061 tgaaagtgaa caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt
     6121 aatggaaagc agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact
     6181 ttatccgcag gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg
     6241 agatttctac agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact
     6301 gttaacgcta atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact
     6361 tatagaacaa acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat
     6421 catagaggca tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga
     6481 acaaaccaga cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca
     6541 attacctgct cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat
     6601 cataaaaaat gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc
     6661 aaatatgcat tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac
     6721 gcacgtattg atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc
     6781 agtcaaactc aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca
     6841 tttgtgatca cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg
     6901 ttagctgaag ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg
     6961 attggaaaag caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca
     7021 aaagagaaaa taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa
     7081 tcatctgagt tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac
     7141 cttcccgatg aggcatggac catcatcaag cccctgttac ctcctgaacc cgccacacca
     7201 cgggccggac gcccatgggc tgagcaccgt aaaatcatca atggcatgtt ctgggtgtta
     7261 tgttccagtg caccatggcg cgatttaccc gaacgatatg gggcatggaa aactgtttat
     7321 aaccgcttta accgatggtc aaagtcaggc gtgattaata ttattttcaa caggttgctt
     7381 tcgctacttg atgcaaacgg ctttattgac tggtctgcca ccgcgctgga tggcagtaat
     7441 atccgggcgc tgaaatgtgc cgccggtgct caaaaaaaca tcccgatatc gacggagata
     7501 atgggccggg tcgctctcgc ggcggttttg gcaccaaaat ccatctggca acagacggaa
     7561 gtggcctccc gttaaatatc gtgctaagcc ccggacaagc tcatgaaagc cagttcgcgc
     7621 aacgacttct ggacgggatt ggcgttcagc gtcagaacgg cagcatgaaa cgccgtggac
     7681 atgcggtact ggctgataaa gcgta
//
LOCUS       DAJTUM010000154         7609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTUM010000154 DAJTUM010000000
VERSION     DAJTUM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO471_003785"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO471_003785"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6244883.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO471_003786"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO471_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6244884.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO471_003787"
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO471_003787"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6244885.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4131..4886
                     /locus_tag="OO471_003788"
     CDS             4131..4886
                     /locus_tag="OO471_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6244886.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="OO471_003789"
     tRNA            4965..5038
                     /locus_tag="OO471_003789"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="OO471_003790"
     CDS             5173..6783
                     /locus_tag="OO471_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6244887.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7422
                     /locus_tag="OO471_003791"
                     /pseudo
     CDS             6815..7422
                     /locus_tag="OO471_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1935 a         1862 c         1955 g         1857 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccat
//
LOCUS       DAJTUM010000155         7474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTUM010000155 DAJTUM010000000
VERSION     DAJTUM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OO471_003792"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OO471_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OO471_003793"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OO471_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6244888.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OO471_003794"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OO471_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OO471_003795"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OO471_003795"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6244889.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OO471_003796"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OO471_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6244890.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO471_003797"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO471_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6244891.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OO471_003798"
     CDS             complement(6511..7203)
                     /locus_tag="OO471_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6244892.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1899 a         1809 c         1969 g         1797 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggtg ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggga taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cggggttctc
     7441 ctgagaattc cgacagaaaa agcaaaaata aatg
//
LOCUS       DAJTUM010000156         7387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTUM010000156 DAJTUM010000000
VERSION     DAJTUM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..190
                     /locus_tag="OO471_003799"
     CDS             <1..190
                     /locus_tag="OO471_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6244893.1"
                     /translation="SLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPP
                     NESENRYWKNSNSVASFC"
     gene            complement(214..907)
                     /gene="ospZ"
                     /locus_tag="OO471_003800"
                     /pseudo
     CDS             complement(214..907)
                     /gene="ospZ"
                     /locus_tag="OO471_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            963..1115
                     /locus_tag="OO471_003801"
                     /pseudo
     CDS             963..1115
                     /locus_tag="OO471_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2496..3443
                     /gene="icsP"
                     /locus_tag="OO471_003802"
     CDS             2496..3443
                     /gene="icsP"
                     /locus_tag="OO471_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6244894.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(4005..4377)
                     /locus_tag="OO471_003803"
                     /pseudo
     CDS             complement(4005..4377)
                     /locus_tag="OO471_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4621..4740
                     /locus_tag="OO471_003804"
                     /pseudo
     CDS             4621..4740
                     /locus_tag="OO471_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4990..5856
                     /gene="ospB"
                     /locus_tag="OO471_003805"
     CDS             4990..5856
                     /gene="ospB"
                     /locus_tag="OO471_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6244895.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            6185..6925
                     /gene="phoN2"
                     /locus_tag="OO471_003806"
     CDS             6185..6925
                     /gene="phoN2"
                     /locus_tag="OO471_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6244896.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7285..>7387)
                     /locus_tag="OO471_003807"
     CDS             complement(7285..>7387)
                     /locus_tag="OO471_003807"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6244897.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2310 a         1401 c         1435 g         2241 t
ORIGIN      
        1 gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg aggcagctca
       61 cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg aatataacgg
      121 tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact cggtggccag
      181 tttttgttga ccacttcata caattaatat acattaagta acaggcattc gagccttgat
      241 ttgagaaagc ctgtcattca tttgttttat gtagctatct atttcctctg atgcataact
      301 aaaaagttct cccatttcac gagcaataat aatctcagat ttaatagact ttaatctctg
      361 gcgactcatc tcttaacact tgcataatct ctctttttat atcatatagt tctataaacc
      421 gatttgcccg tagttctcct tgggaagaat catttctgaa tacaacaatt atatacaaaa
      481 aatcactatt ttctttagag aagatcttac aactaatctc tgggagctca cataatgcat
      541 gtaagtcagg atggtacaaa ctatccacag aaaattgccc cataggacgt tttccagata
      601 taactgtcaa ccagcttcct tccggtgtac tggcttcggc tctggaaata ggctgaaaat
      661 cttccggaca tggagaatac attgtcttca tataattaag tcgtctggat ttctcattaa
      721 taaactcctc tatttttaat tgtgccatac ttctggttat accagagata tacttcttgt
      781 catatatgtt tggtgattca ttaaagtaac catgttcaac tctaggataa atatttctca
      841 ctatatattc agtatttgca gtatttattg gaaatacgtt cttaatattc ttgatgggac
      901 taatcatttt aatctctata ctcaaatata aacattacca tgaacaatat aattgttcct
      961 aagtggaatg tctccacgga gaacacttta tctatcggga aatagtgcgg gcaacagtgt
     1021 ttaattatat cgagtgcaat tacaatcgct ggcgacggca ccgttggtgt ggcggcctca
     1081 gtccggaaca atttgaaaac cagaacctcg cttaggcctg tgtccatatt acgtgggtag
     1141 gctcacagag cctcacttac gactgcgtcc atattatgtg ggtaggatca ataaagacgt
     1201 tttatatgtg tctgcttgca gccagaaaat ttataccctg caggtatcat aaacatgctt
     1261 gataacttaa ttggggctcc ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc
     1321 ggggatttca gtatgaaatg aagtatattt aatatacttt acaaaaagtt tgtattagtg
     1381 gaataaggac aatagcttat ccaaactgcc aagcctcttt attataagta agatctggca
     1441 ctggaatatt ctcaaaagga ctaacatcat caccacgacc ttcaaccaac cattttattg
     1501 catttaaatc ccagaatagt tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg
     1561 ttacgttatc gcaatagagt gaactctcgc cctatacggc gttttcaagg attagtcctt
     1621 aatcggacaa ccacattaaa gcataataaa taattaagct ttaataatat actatagata
     1681 cagttctatt ataaaaaata ctattttagg tgatgcatta aattagtaga atatttattc
     1741 ttttataaaa gcaccaatga attgtccaag taaacatgag ataaaatgtg tagaggataa
     1801 ctgagtttgg tcactttaac tgtattagtc gcagtctgac tctcgacttt aaaaggatgg
     1861 gaattacctg tactgatagt gagaaagctt tccattgaaa taagtcaaat aaaatttgat
     1921 ggcttcagtg ctaaagtgag gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt
     1981 attgcttctg ccatttccat aatatttcac gggaaaaaca aaatctgaaa aactatactt
     2041 aagtgttgta ttgagtcagt tgagatacaa atacatattt atcagcacac gttacgtcaa
     2101 atacaggagg ttgtcatgtt ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca
     2161 atttattgtc ttatcgttgt tcttatggaa cgcttttctg attgatttca tattggcgag
     2221 agaacaagaa ggtgagtaga attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa
     2281 caccaaaagg taggtagaga aatctatggc ccccgttact ttattcgcac aatgaggtat
     2341 ttttttattt ttacaaaagt tcaataaata ttataataaa agatatgttc ttggtaacat
     2401 aaacaaatat ttaattaaaa taatgcttgg cattgacgtt tatcttataa agtaagaaga
     2461 tcatggacat ttcaaccaaa aaagtagagt tctcgatgaa attaaaattc tttgtacttg
     2521 cactttgtgt acctgcgatc tttactacac atgctaccac taactatcca cttttcatac
     2581 cggacaacat cagtacggat attagtctag gatctctgag tggcaaaaca aaagaacgcg
     2641 tttatcatcc caaggaaggg gggagaaaaa ttagtcaact ggactggaaa tacagtaatg
     2701 cgactattgt tagaggtggc atcgattgga agctaattcc aaaagtgtct ttcggagttt
     2761 ccggttggac tactttaggt aaccagaaag caagcatggt tgataaagac tggaacaatt
     2821 ccaatacccc tcaggtatgg accgaccaga gctggcatcc caatacgcat ctccgtgatg
     2881 ctaacgaatt cgagctgaat cttaaaggtt ggttattaaa taatttggat tatcgacttg
     2941 gactaatagc aggttaccaa gagagtcgtt acagttttaa tgcaatggga gggagttata
     3001 tttatagtga aaacgggggg agcaggaata aaaaaggggc acatcctagt ggtgaaagaa
     3061 caataggtta caaacagctc ttcaaaatac cttatattgg attaactgct aattaccgcc
     3121 atgagaattt tgagtttgga gcagaactga aatatagtgg ttgggttctt tcatctgata
     3181 cagataaaca ctatcagact gagacaattt ttaaagatga aataaaaaac caaaattact
     3241 gctctgttgc tgcgaatatt ggatactatg tcacccccag tgcaaaattt tatatagaag
     3301 gctccagaaa ttacatttct aataaaaaag gtgatacatc tctttatgag caaagtacca
     3361 atatatctgg caccattaaa aatagtgcaa gtattgaata tattggtttt ctcacttccg
     3421 caggtataaa gtatattttt tgaattggtt caccgagttg attacgtttt catggctaaa
     3481 aattgccgcc cattgtgagt catattcgcc ctgactttcc agaaccatac gaactgcccg
     3541 ttgacggact tcgggggaaa aacgtgtatt tttagtcatt ctgtttacct ctttctcagg
     3601 gcgtttagtc tccaggattt ccggggcggt tcaaaaaact cttcgttgat tactgtggac
     3661 ttactgttgg cattaccgac cctgagaccg gagaaataag aaccgcttag atcatcgtgg
     3721 ctgttctcgg ggtatcaagt tacacatggg cagaggctac ccaaggtcta ctattatcgc
     3781 ctgaaagcgc tgatggaaca gtgctatcag ggacatgctg atgaaaatac ctaaataatc
     3841 agccagcact ctatctttcc aaatccacag catagcaaag agagcaaaag agcctgtaaa
     3901 ttcagaaatt aaggaaacgc ctcttggtgt acataatcta tcttatgtta aatgaaatct
     3961 aatttcttga ttatatgctg gatcgttcgt gggtggcttt gcccctatat ttccagacac
     4021 ctgttatcat tggcccgctg ccgcagatat tcccgtggcg agcgataatc cagcgcacta
     4081 tgcggatgcc attcgttata atgctcgaat gcctctgcaa ggttctttgc tgccgttaac
     4141 ccgtcaggtt tgggcatgat actgatgtag tcacacttta ttgttttcac gaagctcttt
     4201 gctattctgt tactctccgg actccgcacc gccgtgttct tcggttcaac aaagtcccaa
     4261 catccgggtg aactggcgtg tttcattagc tcggtagcat gaaccattat ccgtcagtca
     4321 cttcactgga gacgccggaa gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc
     4381 gccaacggga gagcatctac ggtctgattg aactgtttat tcaaattgtc caccgtctgt
     4441 cagtacgctc tgaacgtcgg ctggttaaaa cactgctcgc tgattttcag agggttcacg
     4501 gtaaaacagc acttttattc cggattgcgg aagcggctct gaataatccg gatgggctgg
     4561 ttaaagaggt tgtgtaccat ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt
     4621 catcatactt ggcgcagtta cgcgatgttt ctgctagagg tgatgccgta aagaacggca
     4681 gaacattacc ggaacaagat agcggtctac ctgcactggt atcagacccg gggcttcccc
     4741 ggatgatctc cacggtactt taggtactgt gtctatatta tgtaggtagg atcaattaac
     4801 tttgtagttg aaattctttc attgaaataa tgaataaaaa agacaaaccg cattaaacaa
     4861 tgccagaata taattgcatt tttgttccgt ttttttaaat catagtatac aaagaagctt
     4921 gcatactata tgaaaatata acacccttta ctatgatgtt tataaaacaa tatatggagt
     4981 catgtaggta tgaatttaga tggtgttaga ccatactgta gaatagtcaa taaaaagaat
     5041 gaaagcatat cagatattgc atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt
     5101 actcacccaa aagcagcatt ggttttttta ggagagaaag gtttttgtga tagcaatgat
     5161 gttctatcta ttatgggaca acaaatacca agagtattta agaacaagat gttatatgat
     5221 tatgttttta aaaatgaaaa aagtaaaaat gattttctaa aaatggctga atcatggcta
     5281 ccacagagtg aaccaatagt aataaataat gatgatgacg cattgaatgc tgctgcttat
     5341 ttttctgtaa aaaaagcgaa aataaaaaca gtaaacgata ctgattttaa agagtataat
     5401 aaggtttata ttcttgggca cggtagtcct ggttctcatc aattaggcct tggttcggaa
     5461 cttattgatg tacaaacaat catttcaaga atgaaagact gtggtattct aaatgtgaaa
     5521 gatatccgtt ttacttcatg cggctccgct gataaagtgg ctcctaaaaa ttttaacaat
     5581 gcccctgctg aaagtctttc ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa
     5641 tctttgctag agcagataaa aaaacacctt gaaaacgatg agtcattgag tgatggtcta
     5701 aaaatatccg gctatcatgg atatggagtt cactatggtc aagagctttt tccctactca
     5761 cattatcgtt caacttcaat tcctgctgat ccggagcata cagtaaaaag aagctctcag
     5821 aaaaagactt ttattattaa taaagaactg gattagtata aaatttttaa cctatagtta
     5881 attgtttgca tttaagatga agttatttta tctttagtag aaaagtgctt ccctatgagg
     5941 gaaaatcaca aatcatcata atacagagac aaaacgatac gaaaaaatag ataaaaaaca
     6001 tcattctttt accacattat tttccgtgaa tatgaaaaat aatgttatta ctttaatata
     6061 agactatttt ttgtttttcc atcactctgt tcaaattttt ccgcatgact tgtgtttttt
     6121 gtaatacagc tcgtttttta cagctgacca aaatcatcaa ttaattatgc taaggaaata
     6181 aattatgaaa accaaaaact ttcttctttt ttgtattgct acaaatatga tttttatccc
     6241 ctcagcaaat gctctgaagg cagaaggttt tctcactcaa caaacttcac cagacagttt
     6301 gtcaatactt ccgccgcctc cggcagagaa ttcagtagta tttcaggctg acaaagctca
     6361 ttatgaattc ggccgctcgc tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc
     6421 atactacgag aattttggtc ttgcattttc agatgcttat ggcatggata tttcaaggga
     6481 aaatacccca atcttatatc agttgttaac acaagtacta caggatagcc atgattacgc
     6541 cgtgcgtaac gccaaagaat attataaaag agttcgtcca ttcgttattt ataaagacgc
     6601 aacctgtaca cctgataaag atgagaaaat ggctatcact ggctcttatc cctctggtca
     6661 tgcatccttt ggttgggcag tagcactgat acttgcggag attaatcctc aacgtaaagc
     6721 ggaaatactt cgacgtggat atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg
     6781 gcaaagcgat gtagaggctg ggcgtttaat gggagcatcg gttgttgcag tacttcataa
     6841 tacacctgaa tttaccaaaa gccttagcga agccaaaaaa gagtttgaag aattaaatac
     6901 tcctaccaat gaactgaccc cataaagctg gacagcctgt atcaggctat ggagggccca
     6961 tagacaaatc taccctatat gagcatagga ggagtctatg ggcacaccac gttttacccc
     7021 tgaatttaag ggattactgg aaaggctggg acatatccct ccggcagaag cagaaaaagc
     7081 ttattatgct gccatcggaa acgatgatct ggcaacctga gttcacagat aaaacattct
     7141 ctaggaaact cggggcggtt ccgttcacca catgcaatgt ggtgttgcag gggaacggtc
     7201 tgcaccatcc cctatgtcga tcaatataac agaaatgaca acttcagatt cagggcacaa
     7261 cctaaatata ttttaggtca cctctcaaat cgtttgcctg ataccgctcc attttttaat
     7321 aaaaaaatca atcatttttg aatctttgct tttcattgca ttgtcaagca tagtgtcccc
     7381 actattt
//
LOCUS       DAJTUM010000157         7375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTUM010000157 DAJTUM010000000
VERSION     DAJTUM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            51..1541
                     /locus_tag="OO471_003808"
     CDS             51..1541
                     /locus_tag="OO471_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6244898.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO471_003809"
     CDS             1593..2750
                     /locus_tag="OO471_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6244899.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO471_003810"
     CDS             complement(2716..2838)
                     /locus_tag="OO471_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244900.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO471_003811"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO471_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO471_003812"
     CDS             4764..7124
                     /locus_tag="OO471_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6244901.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="OO471_003813"
     CDS             7181..>7375
                     /locus_tag="OO471_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244902.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1615 c         1568 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAJTUM010000158         7173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTUM010000158 DAJTUM010000000
VERSION     DAJTUM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(296..1198)
                     /locus_tag="OO471_003814"
     CDS             complement(296..1198)
                     /locus_tag="OO471_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6244903.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO471_003815"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO471_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6244904.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO471_003816"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO471_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6244905.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO471_003817"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO471_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6244906.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO471_003818"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO471_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6244907.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO471_003819"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO471_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1676 a         1809 c         1871 g         1817 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggt
//
LOCUS       DAJTUM010000159         6936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTUM010000159 DAJTUM010000000
VERSION     DAJTUM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..573)
                     /gene="istA"
                     /locus_tag="OO471_003820"
     CDS             complement(<1..573)
                     /gene="istA"
                     /locus_tag="OO471_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR6244908.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVSREVLYDNMKTVV"
     gene            complement(660..1021)
                     /locus_tag="OO471_003821"
                     /pseudo
     CDS             complement(660..1021)
                     /locus_tag="OO471_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1360..1914)
                     /locus_tag="OO471_003822"
                     /pseudo
     CDS             complement(1360..1914)
                     /locus_tag="OO471_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2057..2179
                     /locus_tag="OO471_003823"
                     /pseudo
     CDS             2057..2179
                     /locus_tag="OO471_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2441..3007)
                     /gene="ipgB2"
                     /locus_tag="OO471_003824"
     CDS             complement(2441..3007)
                     /gene="ipgB2"
                     /locus_tag="OO471_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6244909.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3289..3603)
                     /locus_tag="OO471_003825"
     CDS             complement(3289..3603)
                     /locus_tag="OO471_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244910.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3681..3791)
                     /locus_tag="OO471_003826"
                     /pseudo
     CDS             complement(3681..3791)
                     /locus_tag="OO471_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4115..4792)
                     /gene="ospD1"
                     /locus_tag="OO471_003827"
     CDS             complement(4115..4792)
                     /gene="ospD1"
                     /locus_tag="OO471_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6244911.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5164..5301)
                     /locus_tag="OO471_003828"
                     /pseudo
     CDS             complement(5164..5301)
                     /locus_tag="OO471_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5383..5681)
                     /locus_tag="OO471_003829"
                     /pseudo
     CDS             complement(5383..5681)
                     /locus_tag="OO471_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6270..6429)
                     /locus_tag="OO471_003830"
                     /pseudo
     CDS             complement(6270..6429)
                     /locus_tag="OO471_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6433..6936)
                     /locus_tag="OO471_003831"
                     /pseudo
     CDS             complement(6433..6936)
                     /locus_tag="OO471_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1850 a         1568 c         1444 g         2074 t
ORIGIN      
        1 aaccacagtt ttcatattgt catacaacac ttcgcgcgac acaccaccaa agaagcggaa
       61 cgcattacga tggcaggtct ccagcgtgtc ataacgcata ttgtcagtga attcgatgta
      121 caacattcgg ctgtatccgg gaacagcaac gaacacgtga agcggtgagc gaccattacg
      181 catagtgccc cagtcaacct gcatctgtcg tccgggttca gtttcgaacc gaacggcagg
      241 ctcctgctcc tgaggaaccg agagagaacg aatgaatgcc ctgagaatgg tcattccgcc
      301 acgatatccc tggtctctga tctcgcgagc gattaccgtt gccgggattt tgtaaggatg
      361 agcatcggcg atgcgttgac gaatataatc ccggtattca tccaggagtg aagcaacagc
      421 aggtcgcggc gtatattttg gcggctcaga ttttgcctgc aaataacgtt taacggtatt
      481 gcgggagagc cccagttctc tggcaatcgc ccggctactc attccctgct tgtgcaggat
      541 tttaatttcc ataactgtct caaaagtgac cataagctct cctgaatcag gagagcagat
      601 taccccctgg atctgatttc aggcgttggg tgtggatcac tattgcaccg ttcgtgacag
      661 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
      721 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      781 gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt
      841 tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag
      901 tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct
      961 gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca
     1021 tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
     1081 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
     1141 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
     1201 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
     1261 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
     1321 gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
     1381 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
     1441 ttcgatataa ttaaacactg ttgccctcat tatttcccgg ctgataaagt gttctccgtg
     1501 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
     1561 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
     1621 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1681 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacataga
     1741 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1801 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1861 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1921 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1981 gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct
     2041 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     2101 gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat
     2161 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     2221 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     2281 aggggagagg gatcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     2341 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     2401 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat
     2461 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     2521 tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc
     2581 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     2641 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct
     2701 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2761 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2821 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2881 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcca
     2941 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     3001 caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc
     3061 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     3121 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     3181 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     3241 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     3301 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     3361 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     3421 actgttgctg ataaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     3481 aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc
     3541 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     3601 catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc
     3661 cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3721 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact
     3781 gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac
     3841 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag
     3901 tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt
     3961 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     4021 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     4081 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     4141 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     4201 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     4261 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     4321 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     4381 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     4441 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     4501 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     4561 tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac
     4621 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4681 cgttaatgat attatttttc attcctttat tttcattagc agtattgctg cctattatta
     4741 agtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4801 atcctttttg ctaaaaaaaa acaataacct atttttgtaa tgtaatagat attagtctat
     4861 tgctaatcta aaaaaaccat tgccacattg aatggctata ttcaccaata aattctttcg
     4921 atttcgtata ttgacatatc gtagatatat cttatcccta agattcaata atcgagtccg
     4981 tttaccattc tgtgcaagcg ccttcactca gaagtgaccg tcaaggcgat caccaaactt
     5041 gataataaag cagctcattg ccatacgcca gtccttaagg ggcgttgtcc atttctgtga
     5101 tgctgcctag atttaactgc cagctccggc ttatctacat ctaaatatgt gagcctaata
     5161 ccgctacact ttgtccagac tatgtttttc tccgggaagt gggctaaccg tctccataaa
     5221 gtgacgaact tttttcaaca gtggccatat tgaacggcac tgatgattac gggttattgt
     5281 ctcatgaaga gccggccaca tcactgacag cagtgacttc aggccttcag caccagacag
     5341 cctgaaccag ttaatccatc gcccgacaaa tttagtaatc tagggctggt ttttcttcag
     5401 ctgttgcggt tcgaaagtgc cattacgatc acgcagagta cggatttcca gaggttcatc
     5461 tccagtagtg ataatcttcg ttaagcaacc attgcgggaa ttgctgccag atctaagctg
     5521 atttttctcg cacccaagat gatgagtcat attagcattg agtacggttt cattttcagc
     5581 atccaatcaa actgactgag atcttcagga tttttgagac ttttggctag ttcattagct
     5641 aaagcccgca acttattaga tatcaactgt ttttcattca taaattcacc ttcatttgat
     5701 atcaaattaa atagttcaac atcagagaat tatacaaact tatgtacagg ctcctatgat
     5761 cttcgaatcc tgaagattat agtgaaaata agttttcacg ttgaaatccc acatagttag
     5821 tatatgataa ataatcatgg atgacctgat taacctcttg gggtagtgct tttatagaag
     5881 aataaatatt ctactaattt aatacatcac ctaaaatagt attgtttttt atggttggtt
     5941 gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc cagtgccaga tcttacttat
     6001 aataaagggg cttggcagtt tggataaact attgttctta ttccactaat acaaactttt
     6061 tgtaaagtat attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa
     6121 caattacacc ataaaaggaa gagggggccc taattaagtt gtcaagcatg tttatgatac
     6181 ctgcagggta taaattttct ggctgcaagc agacacatat aaaacgtctt tattgatcct
     6241 acccacataa tatggacaca gtcgtaagtg aggctctggt tttcaaattg ttccggactg
     6301 agaccaccac accaacggtg ccgtcgccag cgattgtaat cgcactcgat ataactaaac
     6361 actgttgccc gcactatttc ccgatggata atagtgttct ccgtggagac attccatttt
     6421 cagcgaatga attttcatct gttgctgtat ccagtcatac agtgactgca tcagtggcgc
     6481 ggctctggct tttcttgccg ccagacgctg ttctgctgaa catccccgga cttctgcctc
     6541 gatggcatac agttcaccga tacgctgcag ggcttccgtg gtgatgtcgg ttggcactct
     6601 tgcatgcaca tcgtggattt ttctccggac atgagccata catgcggctt ccgttattct
     6661 gccggattcg tacaacgccc ggtaaccacc gtaagcatcg gcctgaagca ctccgctgta
     6721 accggccaga tgattctgtg gatggatacc tttccggtcc ggtgagtacg cgaaccagac
     6781 cgccggaggc atttccgaac cggcgttacg gtcatcacga acgtagaccc acagccgggc
     6841 agtccttgtt ttaccgctgc ccggctcctg gaccggaacg gggatatcat cggcatggac
     6901 tttaccgggc atcagcacat actggcgcag gatgtc
//
LOCUS       DAJTUM010000160         6790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTUM010000160 DAJTUM010000000
VERSION     DAJTUM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO471_003832"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO471_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6244912.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO471_003833"
     CDS             2089..2238
                     /locus_tag="OO471_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244913.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OO471_003834"
     CDS             complement(3005..3445)
                     /locus_tag="OO471_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6244914.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OO471_003835"
     CDS             3663..4052
                     /locus_tag="OO471_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244915.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="OO471_003836"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="OO471_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="OO471_003837"
     CDS             complement(4607..5443)
                     /locus_tag="OO471_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6244916.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="OO471_003838"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="OO471_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /locus_tag="OO471_003839"
     CDS             complement(5793..6266)
                     /locus_tag="OO471_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6244917.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="OO471_003840"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="OO471_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1585 a         1757 c         1771 g         1677 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaatgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt cttatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DAJTUM010000161         6627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTUM010000161 DAJTUM010000000
VERSION     DAJTUM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            240..2672
                     /locus_tag="OO471_003841"
     CDS             240..2672
                     /locus_tag="OO471_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6244918.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO471_003842"
     CDS             2739..4208
                     /locus_tag="OO471_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6244919.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO471_003843"
     CDS             4208..5869
                     /locus_tag="OO471_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6244920.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO471_003844"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO471_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6244921.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT         1953 a         1569 c         1524 g         1581 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcgg
//
LOCUS       DAJTUM010000162         6523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTUM010000162 DAJTUM010000000
VERSION     DAJTUM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO471_003845"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO471_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6244922.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIAMIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO471_003846"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO471_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6523
                     /gene="narH"
                     /locus_tag="OO471_003847"
     CDS             5646..>6523
                     /gene="narH"
                     /locus_tag="OO471_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6244923.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1691 g         1561 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc atgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct cta
//
LOCUS       DAJTUM010000163         6256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTUM010000163 DAJTUM010000000
VERSION     DAJTUM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO471_003848"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO471_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6244924.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO471_003849"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO471_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6244925.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO471_003850"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO471_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO471_003851"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO471_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1458 c         1477 g         1662 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgatg
//
LOCUS       DAJTUM010000164         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTUM010000164 DAJTUM010000000
VERSION     DAJTUM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..312
                     /locus_tag="OO471_003852"
     CDS             <1..312
                     /locus_tag="OO471_003852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6244926.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OO471_003853"
     CDS             complement(388..852)
                     /locus_tag="OO471_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6244927.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO471_003854"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO471_003854"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6244928.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OO471_003855"
     CDS             complement(1721..2410)
                     /locus_tag="OO471_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6244929.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO471_003856"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO471_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6244930.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO471_003857"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO471_003857"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6244931.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO471_003858"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO471_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6244932.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1584 a         1695 c         1553 g         1423 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatag tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 acaatgggta atgct
//
LOCUS       DAJTUM010000165         6184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTUM010000165 DAJTUM010000000
VERSION     DAJTUM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..173
                     /locus_tag="OO471_003859"
                     /pseudo
     CDS             1..173
                     /locus_tag="OO471_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            698..1876
                     /gene="ltrA"
                     /locus_tag="OO471_003860"
     CDS             698..1876
                     /gene="ltrA"
                     /locus_tag="OO471_003860"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6244933.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1959..2138
                     /locus_tag="OO471_003861"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="OO471_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2168..2650)
                     /locus_tag="OO471_003862"
                     /pseudo
     CDS             complement(2168..2650)
                     /locus_tag="OO471_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2906..3886)
                     /locus_tag="OO471_003863"
     CDS             complement(2906..3886)
                     /locus_tag="OO471_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6244934.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="OO471_003864"
     CDS             complement(3886..5085)
                     /locus_tag="OO471_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6244935.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5407..5745)
                     /locus_tag="OO471_003865"
                     /pseudo
     CDS             complement(5407..5745)
                     /locus_tag="OO471_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5812..>6184)
                     /gene="istB"
                     /locus_tag="OO471_003866"
     CDS             complement(5812..>6184)
                     /gene="istB"
                     /locus_tag="OO471_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6244936.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1674 a         1425 c         1475 g         1610 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgttgtgtca acgacggatg
     5761 aaaagtgatc cacttatatc tccaccaacg gcccaatatt gatccaccgt tttactcagg
     5821 attagcttct gctataaccc cggcctttcg tttctgtctg agtcgatagc tttctccttt
     5881 gatttgaacg acatgtgagt ggtgtaagat acggcccagc atcgctgagg tcagtgctgc
     5941 atcaccggcg aacgtttgat cccactgccc gaacggtaga ttggatgtca ggatcattgc
     6001 gctcttttcg taacgtttag cgatgacctg gaagaacagt tttgcttctt cctgactgaa
     6061 cggcagatag cctatttcat caatgatgag caggcggggg gccattactc cacgctgaag
     6121 cgtcgtttta taacggccct gacgttgtgc cgtagataac tgaagtaaca gatctgctgc
     6181 tgtt
//
LOCUS       DAJTUM010000166         6157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTUM010000166 DAJTUM010000000
VERSION     DAJTUM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..171
                     /locus_tag="OO471_003867"
     CDS             <1..171
                     /locus_tag="OO471_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6244937.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO471_003868"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO471_003868"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO471_003869"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO471_003869"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6244938.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO471_003870"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO471_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6244939.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO471_003871"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO471_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6244940.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5426..6157
                     /locus_tag="OO471_003872"
                     /pseudo
     CDS             5426..6157
                     /locus_tag="OO471_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1578 a         1310 c         1558 g         1711 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcatg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta ttttaaaatg gttaagtttt gctaatcatg atgagttatt
     5341 tttgatagtt catcctgtgg cgtcgctctt aaacttatat tgtaaatgac ttagatttaa
     5401 ataagataat aattttgtta acaatatgag gcgggagatg ttttctacaa acttaataca
     5461 gagtaattat ggtgatttaa atattaaaag cctggctttc gactctttta aagaaaggct
     5521 tgagtctacc atggcggcac tcactttctt tatatcaacg ggtcaatgtg actgcgatga
     5581 aatagcagag tcgaacttca atcgtatgat tgcctatatg tctaacatta actatgatgc
     5641 ctcaaagcta ggagcgccag cacttagttt tgatacgtat ctgcaggaca atgttaaata
     5701 tagagtgata attaataatc tttatggcag tgagtttcgg ataagagata ttaatgagaa
     5761 ttttgttggg atggatttta cctgtgaatt ccgacccgaa aaaatgacga atttgatcaa
     5821 tataaaaaat aggttggtta ttcattattt aaggactata tactatgaaa aaaattatat
     5881 acatcctgcc gggagcattt ttgctgcgat tcaaaaaaaa tgagagtctc ctaaagtttc
     5941 cttcgataat atcaatgctt aatatcaatc ttctttttaa tccactaaat cttccgggaa
     6001 tgggaagtgg tgtgcttgaa gatattatga gtattccaga tagttcactt agaaaacgtc
     6061 tcgggtatga ggttttaagc ttctcactac aggcacattc attgagccag gaatgtattg
     6121 ataagttgga catttttttc gctgacgatc tgtttaa
//
LOCUS       DAJTUM010000167         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTUM010000167 DAJTUM010000000
VERSION     DAJTUM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..143)
                     /locus_tag="OO471_003873"
     CDS             complement(<1..143)
                     /locus_tag="OO471_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6244941.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="OO471_003874"
     CDS             379..651
                     /locus_tag="OO471_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6244942.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO471_003875"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO471_003875"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6244943.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="OO471_003876"
     CDS             1111..1731
                     /locus_tag="OO471_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6244944.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO471_003877"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO471_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="OO471_003878"
     CDS             complement(3114..3767)
                     /locus_tag="OO471_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6244945.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="OO471_003879"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="OO471_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="OO471_003880"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="OO471_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAJTUM010000168         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTUM010000168 DAJTUM010000000
VERSION     DAJTUM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            430..1338
                     /locus_tag="OO471_003881"
     CDS             430..1338
                     /locus_tag="OO471_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6244946.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO471_003882"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO471_003882"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6244947.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO471_003883"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO471_003883"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6244948.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO471_003884"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO471_003884"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6244949.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OO471_003885"
     CDS             complement(5673..>6052)
                     /locus_tag="OO471_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244950.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJTUM010000169         5891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTUM010000169 DAJTUM010000000
VERSION     DAJTUM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..393)
                     /locus_tag="OO471_003886"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO471_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO471_003887"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO471_003887"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6244951.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO471_003888"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO471_003888"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6244952.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1822 a         1335 c          986 g         1748 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag c
//
LOCUS       DAJTUM010000170         5867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTUM010000170 DAJTUM010000000
VERSION     DAJTUM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..266
                     /locus_tag="OO471_003889"
     CDS             <1..266
                     /locus_tag="OO471_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6244953.1"
                     /translation="ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            263..526
                     /locus_tag="OO471_003890"
     CDS             263..526
                     /locus_tag="OO471_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6244954.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            519..905
                     /locus_tag="OO471_003891"
     CDS             519..905
                     /locus_tag="OO471_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR6244955.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            913..1599
                     /locus_tag="OO471_003892"
     CDS             913..1599
                     /locus_tag="OO471_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6244956.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1577..2200)
                     /gene="tetR(B)"
                     /locus_tag="OO471_003893"
     CDS             complement(1577..2200)
                     /gene="tetR(B)"
                     /locus_tag="OO471_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6244957.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2282..3487
                     /gene="tet(B)"
                     /locus_tag="OO471_003894"
     CDS             2282..3487
                     /gene="tet(B)"
                     /locus_tag="OO471_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6244958.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3600..4193)
                     /gene="tetC"
                     /locus_tag="OO471_003895"
     CDS             complement(3600..4193)
                     /gene="tetC"
                     /locus_tag="OO471_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6244959.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4326..4697
                     /locus_tag="OO471_003896"
     CDS             4326..4697
                     /locus_tag="OO471_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6244960.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4707..>5867)
                     /locus_tag="OO471_003897"
     CDS             complement(4707..>5867)
                     /locus_tag="OO471_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6244961.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1615 a         1096 c         1298 g         1858 t
ORIGIN      
        1 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
       61 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
      121 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
      181 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
      241 atctgaacat aaaacactat caataagttg gagtcattac cgaccactat taagtcatgt
      301 agctggtttt atttcaattg aacgttttca aagtctagct acagaaggaa aaatgttatc
      361 gctatcttgg tgggaaaacg aatacgcagt tctgcaatgg aaaaatcatg ttttacatgc
      421 gaaagctcaa caagaagggc gagagtcaat atttgatttt tacaaaatta gtattgctca
      481 tattactcgc gaatattcat ttaaaaagga caaggataat gtttgatgtt cacgttgttt
      541 tagataatca aataggacaa ttagcattac taggaaaaac attaggtaat aaaggtattg
      601 gattggaagg gggagggata tttacggttg gtgatgaatg ccatgctcat tttcttgttg
      661 aacaaggaaa ggaagctaaa atagcgctag agcaagctgg actgttagta cttgcgatcc
      721 ggacaccatt aattcgtaag ttaaaacagg aaaaaccggg ggaacttggc gaaatagcac
      781 gagtattggc ggagaataac attaatattt tagtgcaata cagtgaccat gctaaccaac
      841 tgatattaat aacggacaat gatagtatgg ctgcatctgt tacgctccct tgggcaataa
      901 agtgaacttg cgatggctaa tttaatacga aaagaggtta cctttgagtc ctcaatagcc
      961 gcgatagggg cggctatgtc tgacatttca cgagttaaaa tactcagtgc tttgatggat
     1021 gggcgagctt ggacggccac tgagctaagt tctgtggcga atatatcagc ttcaacggcg
     1081 agcagtcatt tatctaaatt attagattgc cagctaatca cagtagtagc tcaaggcaag
     1141 catcgttatt ttcggctagc aggaaaagat attgctgaat tgatggaaag tatgatgggg
     1201 atctccttaa accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca
     1261 cgtacttgct atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt
     1321 caacagcaat ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt
     1381 catgaaatgg gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta
     1441 gattggagtg aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt
     1501 tatgaatcta aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa
     1561 aaaggttatg ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata
     1621 tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga
     1681 tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag
     1741 cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga
     1801 gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt
     1861 cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc
     1921 gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc
     1981 tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg
     2041 cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta
     2101 gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta
     2161 atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc taatttttgt
     2221 tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga
     2281 aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg
     2341 ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc
     2401 taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg
     2461 gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg
     2521 cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg
     2581 ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga
     2641 taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct
     2701 tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc
     2761 cttttttatc gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg
     2821 tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa
     2881 ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc
     2941 gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg
     3001 atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt
     3061 ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct
     3121 cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt
     3181 agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg
     3241 agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag
     3301 ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc
     3361 actaccaatt tgggatggct ggatttggat tattggttta gcgttttact gtattattat
     3421 cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag
     3481 tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata
     3541 acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt
     3601 tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta
     3661 tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt
     3721 aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg
     3781 acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa
     3841 ccaatgatag gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa
     3901 tcaagataat gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt
     3961 gttgtgatgt aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt
     4021 tttttatttt taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc
     4081 tcatctattg aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc
     4141 agtgattgat aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat
     4201 ttaaaataac ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt
     4261 aagaacattt aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta
     4321 ataacatcca attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc
     4381 tattatggat tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag
     4441 cgggttatac caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg
     4501 ctagctatat tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa
     4561 aaactatcct tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc
     4621 gatttaagta catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat
     4681 tggaggcaat gcactgagag atcccctcat aatttcccca aagcgtaacc atgtgtgaat
     4741 aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat tgtgtagcca
     4801 gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg atttctgact
     4861 gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc cgcaagccaa
     4921 catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc tgagctgctc
     4981 gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg gaaggtttct
     5041 tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg aatttcaaca
     5101 ggtaagttag ttgctagaac ccatggctcc tttgccgacg ctgagtagat tttaggtgac
     5161 gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga gcgagattta
     5221 tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata gcctaaagtc
     5281 tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt ttccgctcct
     5341 aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc cagcttctca
     5401 acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg tgtggtgtta
     5461 ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt tgaacattgc
     5521 tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag tgcgactgaa
     5581 gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac aagtacaatg
     5641 ggcatcgtat tgcccgaaca gataaagcta gcatgccaac ggtatacagc gagtcgctct
     5701 ttgtggaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg ttttgttctc
     5761 gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca gtcaagtaat
     5821 gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaattc
//
LOCUS       DAJTUM010000171         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTUM010000171 DAJTUM010000000
VERSION     DAJTUM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..241
                     /locus_tag="OO471_003898"
     CDS             <1..241
                     /locus_tag="OO471_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244962.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO471_003899"
     CDS             complement(238..1179)
                     /locus_tag="OO471_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6244963.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO471_003900"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO471_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO471_003901"
     CDS             complement(1684..2904)
                     /locus_tag="OO471_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6244964.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO471_003902"
     CDS             complement(2908..3651)
                     /locus_tag="OO471_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6244965.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO471_003903"
     CDS             complement(3542..5008)
                     /locus_tag="OO471_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6244966.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO471_003904"
     CDS             complement(5008..5241)
                     /locus_tag="OO471_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6244967.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO471_003905"
     CDS             complement(5229..5708)
                     /locus_tag="OO471_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244968.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1225 a         1618 c         1436 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccgggacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTUM010000172         5701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTUM010000172 DAJTUM010000000
VERSION     DAJTUM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..170)
                     /locus_tag="OO471_003906"
     CDS             complement(<1..170)
                     /locus_tag="OO471_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244969.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO471_003907"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO471_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6244970.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO471_003908"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO471_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO471_003909"
     CDS             complement(3616..4383)
                     /locus_tag="OO471_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6244971.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO471_003910"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OO471_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5701
                     /locus_tag="OO471_003911"
     CDS             5484..>5701
                     /locus_tag="OO471_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6244972.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQ"
BASE COUNT         1410 a         1469 c         1480 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 a
//
LOCUS       DAJTUM010000173         5602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTUM010000173 DAJTUM010000000
VERSION     DAJTUM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..411
                     /locus_tag="OO471_003912"
     CDS             <1..411
                     /locus_tag="OO471_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6244973.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO471_003913"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO471_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO471_003914"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO471_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6244974.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc ga
//
LOCUS       DAJTUM010000174         5591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTUM010000174 DAJTUM010000000
VERSION     DAJTUM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..279)
                     /locus_tag="OO471_003915"
     CDS             complement(1..279)
                     /locus_tag="OO471_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244975.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO471_003916"
     CDS             220..405
                     /locus_tag="OO471_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244976.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO471_003917"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO471_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6244977.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO471_003918"
     CDS             complement(1254..1670)
                     /locus_tag="OO471_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244978.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO471_003919"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO471_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO471_003920"
     CDS             1837..2862
                     /locus_tag="OO471_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6244979.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO471_003921"
     tRNA            complement(2915..3004)
                     /locus_tag="OO471_003921"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO471_003922"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO471_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6244980.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO471_003923"
     tRNA            3996..4071
                     /locus_tag="OO471_003923"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OO471_003924"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OO471_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1509 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct a
//
LOCUS       DAJTUM010000175         5588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTUM010000175 DAJTUM010000000
VERSION     DAJTUM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            50..244
                     /locus_tag="OO471_003925"
     CDS             50..244
                     /locus_tag="OO471_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6244981.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="OO471_003926"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="OO471_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6244982.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="OO471_003927"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="OO471_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6244983.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="OO471_003928"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="OO471_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6244984.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="OO471_003929"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="OO471_003929"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6244985.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO471_003930"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO471_003930"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6244986.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1310 a         1376 c         1545 g         1357 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga ataaggtgca cagtatacct gaagcggggc aggagtgttt tacgaaagtt
     5041 gtcgcgatgt tgacaagaag agaatgaaag agagaccagg acatctttgt aatagaaata
     5101 gcaataattt catattcact gaaaatattt ttaatcttta tttatagctc ttggttatta
     5161 ttttttggcg tttggttgtg ctgctataaa ttgaataatt caaactttgt tgtatattga
     5221 taagatattt atctggtttg tatttttgat atttataacg cgtatgcatc aaaaataaca
     5281 ttatctgcaa catactgaaa tatcgtcctt ttatcaattt gatgcgtgtt tttctccata
     5341 aattatatat gtccacattc ggacttaggg gaaagaataa ttgaaccatt cgtctgtaac
     5401 gcagcataat cgttagcgcg aaacataata tgttttctat gcattgataa ttgatggatc
     5461 aacttattac gtccctgagg agggatgaca aatgcactcc tggaaaaaga aacttgtagt
     5521 atcacaatta gcattggctt gcactctggc tatcacctct caggctaatg cagcgaccaa
     5581 cgatattt
//
LOCUS       DAJTUM010000176         5566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTUM010000176 DAJTUM010000000
VERSION     DAJTUM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..113)
                     /locus_tag="OO471_003931"
     CDS             complement(<1..113)
                     /locus_tag="OO471_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6244987.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQR"
     gene            complement(110..643)
                     /locus_tag="OO471_003932"
     CDS             complement(110..643)
                     /locus_tag="OO471_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6244988.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(709..1338)
                     /locus_tag="OO471_003933"
     CDS             complement(709..1338)
                     /locus_tag="OO471_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244989.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1342..1557)
                     /locus_tag="OO471_003934"
     CDS             complement(1342..1557)
                     /locus_tag="OO471_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6244990.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1850..1924)
                     /locus_tag="OO471_003935"
     tRNA            complement(1850..1924)
                     /locus_tag="OO471_003935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1889..1891),aa:Gly,seq:tcc)
     gene            complement(1928..2003)
                     /locus_tag="OO471_003936"
     tRNA            complement(1928..2003)
                     /locus_tag="OO471_003936"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1968..1970),aa:Thr,seq:tgt)
     gene            complement(2006..2082)
                     /locus_tag="OO471_003937"
     tRNA            complement(2006..2082)
                     /locus_tag="OO471_003937"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2046..2048),aa:Arg,seq:tct)
     gene            complement(2084..2160)
                     /locus_tag="OO471_003938"
     tRNA            complement(2084..2160)
                     /locus_tag="OO471_003938"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2124..2126),aa:Ile,seq:cat)
     gene            complement(2360..2740)
                     /locus_tag="OO471_003939"
     CDS             complement(2360..2740)
                     /locus_tag="OO471_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6244991.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(2733..2933)
                     /locus_tag="OO471_003940"
     CDS             complement(2733..2933)
                     /locus_tag="OO471_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6244992.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3028..3285)
                     /locus_tag="OO471_003941"
     CDS             complement(3028..3285)
                     /locus_tag="OO471_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6244993.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(3282..4673)
                     /locus_tag="OO471_003942"
     CDS             complement(3282..4673)
                     /locus_tag="OO471_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6244994.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(4670..5548)
                     /locus_tag="OO471_003943"
     CDS             complement(4670..5548)
                     /locus_tag="OO471_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6244995.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
BASE COUNT         1348 a         1559 c         1220 g         1439 t
ORIGIN      
        1 tcgcgctgct ctttgtaggt aatggcgtta tcacggtaat gattaacagc ccatgacagg
       61 cagacgatga tgcagataac cagagcggag ataatcgcgg tgactctgct cattgatcta
      121 tcccccaaca ggctaatgcg ctttcctggt cacgacgaat aacctgtcca tagcagttat
      181 ttgaacgtat gcggcaatcg cgcccaacat cttttatcca ccagcgaatc gcctcgcatg
      241 cgcccttacg atcaccggca ttcagccgct tataaaacgt cgacgggaaa cacttaccgg
      301 ggccaatgtt ataggggcaa aatgacgcta tacccgcttt ctggggttcg gtcagaggta
      361 ctttaatatt gcgctccacc catgccagcg ccttatcacg ttcaatagcg ttaacctggt
      421 cacatttttc cttcgacagc ttcattcccg gtatgacagg cttaccatcc accattgtgg
      481 caccacgaca gatggtccat atgccagaac catcacggta tgccgttgtg tgattaccct
      541 ctttttcgtt cagaaactgg tcaagtatct ggggagcaga cgcgcctgcg gcaatcagcg
      601 ccagaacagc agccgacagg ccgtatttga tttttgcgct catggatatt tatcaggatt
      661 tatcggcaac aaataacgag ccaacttata tacgcctttt aagataagtc agccccggct
      721 ggatccagtc atcaggctct ttcttaaagg gggagtattg aaatcacgaa gaagagcctc
      781 ccgcaccgca gcatccatgt ctgcaccact ggcaagtgct tcaatctctg ccgccacctg
      841 caaatacccc atgcaacggc caatgcgttt cataactctg gtgttttcgt ctttcaggca
      901 tctgatagcg agattaataa ttttcaggca gtgatgatgc gatgcccgga caggttttcc
      961 gttctcataa acgacgaact caccctcttc acaggaaacc ctgaaaggcc gatggggttc
     1021 ctggtcttta tccggtgcag acgcaataaa atcactttta tgctcttcct ctccggcact
     1081 gttaaccgta atgacgtact caggccgcag gtaagccgcc tctttttctg acaatgcggc
     1141 cggtaaaaac ttttcctgca aaaacttcat aaaagtctcg tgagcgactt tgtggcagta
     1201 atcgtaaatc gccgcataat gctcatcgcg gcgtttgttt tcatcttcag aactcaccaa
     1261 cgccgacaat tttttattaa gctcagtgat ttcattttcc aggtgattga atcgctgatt
     1321 catttcttca tgcttcatta tttactctcc ccgtgcagcc ttacgcttgt cttctcttat
     1381 tttgaaatac agattcgtca gataagtcag aagccccaga agcagacttc ccagcacacc
     1441 aatcgcagcc cactgtgatg gcctgacctg atccaaccac tgcaaaaacc agtagccggc
     1501 actgccggcg gaggtgccgt aggcaatgcc cgttgaaatt ttgtccatgg atttcatagc
     1561 ctcacctccg caaataacgg atggcgtagt tttacactga gaaatgaaag ggatttgaaa
     1621 agaaaaaaac gcaaaagcgg gcgaaacgat atatacagta aggaaagcac tctatccaac
     1681 aaaccaccca cagttaatcg gaataaaagc agagtgctta tgaatgatcg cacgcccgaa
     1741 ggttagtatt tctgcacagc aattttgcaa aaaaaggcga tcattcataa cttaaacgtc
     1801 tttcagtcac tccgggattt cccatcatcg cagactgaaa gactctgact ggagcgggca
     1861 gcgggaatcg aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc
     1921 ccgcatatgg tgccgactac cggaatcgaa ctggtgacct actgattaca agtcagttgc
     1981 tctgcctgct gagctaagtc ggcgctggcc caccaccgag gactcgaacc tcgcaccgtc
     2041 aacttagaag gttgatgctc tatccggatg agctagtggt ggttggtggc ccttgctgga
     2101 cttgaaccag cgacctggcg attatgagtc gctcgctctg accaactgag ctaaagggcc
     2161 ggaggcagaa taataaccat atgtcatcac atctgcaaac tcatctgacc accagcgtgt
     2221 ttaacgtcct gtaccgtttt tcaggcataa aaaacccgcc cggaggcggg tttaagctgt
     2281 gtggcgaagc aaccactctt aacagaatat cccgtttttt acgtacgtaa aatattttct
     2341 agaaagtcgc cccttaccat cagggatgtt caatatattt gtccatttct aaccggacac
     2401 ccaacatcat caacattcct tccaccaccc cctcggcttt ttgtagcttc tttcctatat
     2461 aaccatctga gcatccatgc ttccgcgcca gcgtcatgaa tgtcatccca aacacgtagt
     2521 agtcaaccag caagtcatgt agatcgttgt tgttccggtt aaggcgggcc atacacccac
     2581 atataaccat cgcgtcatcg tcacagcatt gcgggcggga ttttactttt gaagggatta
     2641 atcccttaaa accggcagca atggacgacc aggtcacatc ctcatgatta ttagccaccc
     2701 acgcgcccca gcgctcaaga acctgctgaa tatcacgcat cactgtcttt acccctgtcc
     2761 catccacgat gaacaatcag aacaccatca acaatggcgt gccctttgcc ctctttatct
     2821 tcagcatatt ttcttaccgt ggcgcgatta cagttcagca ttcgtgaaac ttcggtcata
     2881 ttacctcgtg tctggataag cagttccggt atcgtttgaa ttttgacgct catcaaatac
     2941 tctccggttc ggtgattttt atcccgggct ttccaccagg gacataatga tcgcgcacaa
     3001 gccagggcaa aacaaactca tggcgcatca gcgcagcgcc tcctgcacca gtttttcaaa
     3061 ctttccgact ctggtttcca gctctgccac acagtccacc agctcatcta ctgctttctg
     3121 tgcgcgatgc ttcgcctgca tcagttcccg aagcgcgggt accatatctt tacggatagc
     3181 atcttttgtt atgcccgttt tttcgagttg ttcagcatga cgcagcattt cctgcgcgtg
     3241 tttacgcaat tgttcagggg taaaagtcat tgtctggttg ttcaaaagaa acgctccatc
     3301 ttactgctgt cagttcgttt attactgtat ctgcgcggat tgccgggctt catgggagtg
     3361 gaaagcaccc gtgcactttc ctggtccaca ggcagaaaat gtccgttata aaaacgccgg
     3421 taaatcgttc ccagagaacc gttacgttgt ttcgtgatat tgatttctgc gatgcccctg
     3481 gcctgcgtat ccgggttgta cacttcatcc ctgtaaagca tcagaatgat gtctgcatcc
     3541 gcctctattt ctccggaatt tttcaggtct gagttcatgg gacgtttatt gggtctggac
     3601 tccacaccgc gggagagctg gctcagcgca atcaacggaa aaccaccgga ttttgccagg
     3661 cctttaagcc cctttgagat ttcacccacg gcaaggtcat gacgccccgt ggttcgggtt
     3721 tttatcagcc cgagataatc aaccaccacc agcgccgttt ccggatattt aatcaggtgg
     3781 tgtttcgttg ttgcgcatat ctcatcaatg gccaggttcg cctggtccac catccagata
     3841 ttgcgcccgg tcatccgccc caccccttgt gagaaacgcg tccagtcttc atcttcaaag
     3901 tgagccacag atttcaggcg tgatactggc atccctccag ccgcagacac catacgttca
     3961 ccaatctgga tgttcgccat ctccatggtg aacagaagca caccatgccc ctgctcagtc
     4021 accttgtcga tgatgtccag cgcaagttcg gttttcccca tcgaaggacg ggcggcaatg
     4081 aataccaggt ctccgggctc cataccgcct gtttttgcgt ccagttcatc aataccggtc
     4141 atcaacgtcc tggatttctc cagcccctga ttccggcatt caacacgctc aaccacttcc
     4201 ggaagcacat catcaatatg taccggctga atgacgccct ttcctgtcga cagtgtgacc
     4261 atcatgttct gcgcatcctt cagggcatct tcagctgctt cacaggtatg cgcatcacgt
     4321 aatttctgca gcgcctcatt cagtgttttt tctgcatcgc gcagtgcggc attgcgccgc
     4381 aacgctgcaa catagtgctc cagtgaagac ttcacccagg ttttacgccc ggtatcagta
     4441 atcaccgggg caagttccgg catctcatta cacaacagca cggggtcaat cactcctgaa
     4501 acacgggcct gtctgcagat gcctgtatag atatcctgat acgctcgtac agaaaaaacg
     4561 tccgctggca gtgtggccag aatatccatc acttcaggat ctgctccgcg cagaaagaac
     4621 gcgccgatga cagcgccttc aaggtcatcg ttacgccata ctggagttgt catgcagcca
     4681 cacctctgat acgagaacgg taactgggcc agttaaacga caaccagttg cgtcccccgt
     4741 ctgtgatcct gtcggcaatt cggggactga tgaacgccca caactcttcc ggtgagaggt
     4801 tactgatcag aatggtgggc aagatacttt cgtacctggc gttgataatt tcctgcaaaa
     4861 tagccatttc agccgcgctg ccaaactgaa cgccaacttc gtcgatgatc agcaaatcca
     4921 ttgacgcata acgctcaata acttcatccg ctgttttttc gctgtcattc cgccagcaat
     4981 ttttcacagc acgggtaagg cgcatcacgt cggtgatctc cacactggcc agatagttac
     5041 ggatgatgtg ttttgccata gccacagcca gatgattttt tccggtacca caactgccgg
     5101 tcataacaag actggtaccg ttctccagca tatctggcca gttctccgca tagcggcgac
     5161 aggccgcaag atttctggct gcgccaggat taacctccag ataattatca aactcgcagt
     5221 cccgaaaacg cagggcaatt ccggcgttat cagtcagctc ttccgccttg atggacgaca
     5281 gctccatggt caaatcgttg gcctcagcga ttaagcagtc agggcagcat gaaatttctt
     5341 ctctgtcctc gccattacga tcgctccaca ccagtatatg cgtgtgatat tcgccatgtt
     5401 tttcgcaata tccgcgacct tcacgcatca ggcaggaacg ataaggccat ggcttttcgc
     5461 ccttctgagc aaatgcaatc tctgcccgta actcatccat tcgcgcctgt agtctttttt
     5521 gttgttcacg caggttaaac gtcatcatcg ctgtcacctc agaatg
//
LOCUS       DAJTUM010000177         5548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTUM010000177 DAJTUM010000000
VERSION     DAJTUM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..642
                     /locus_tag="OO471_003944"
     CDS             <1..642
                     /locus_tag="OO471_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6244996.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OO471_003945"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OO471_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6244997.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="OO471_003946"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="OO471_003946"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6244998.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1373 a         1358 c         1540 g         1277 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagctat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagc
//
LOCUS       DAJTUM010000178         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTUM010000178 DAJTUM010000000
VERSION     DAJTUM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1630)
                     /locus_tag="OO471_003947"
     CDS             complement(<1..1630)
                     /locus_tag="OO471_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6244999.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO471_003948"
     CDS             complement(1646..2530)
                     /locus_tag="OO471_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6245000.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO471_003949"
     CDS             complement(2541..3146)
                     /locus_tag="OO471_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6245001.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="OO471_003950"
     CDS             complement(3317..>5485)
                     /locus_tag="OO471_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR6245002.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1363 c         1141 g         1400 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccta gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAJTUM010000179         5463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTUM010000179 DAJTUM010000000
VERSION     DAJTUM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            16..558
                     /locus_tag="OO471_003951"
     CDS             16..558
                     /locus_tag="OO471_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6245003.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDAGETELSAR
                     LNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIMASNLLFSLGAS
                     MTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVLYGEMRVGSRVV
                     SQEISTADEGDGGQVVVIGR"
     gene            619..4098
                     /locus_tag="OO471_003952"
     CDS             619..4098
                     /locus_tag="OO471_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6245004.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            4166..4765
                     /locus_tag="OO471_003953"
     CDS             4166..4765
                     /locus_tag="OO471_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6245005.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4829..>5463
                     /locus_tag="OO471_003954"
     CDS             4829..>5463
                     /locus_tag="OO471_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6245006.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         1271 a         1329 c         1724 g         1139 t
ORIGIN      
        1 cgcatcgacc ttcgtgtgaa aacgggggct gaagccatcc gcgcactggc cacacagctc
       61 ccggcgtttc gtcagaaact gagtgacggc tggtatcagg tgcgcattgc cgggcgtgat
      121 gcaggtgaaa ccgaattgtc tgcccgtctt aatgagccgc tggcaaatgg tgcagtgatc
      181 cacatagtac cgcgtctggt gggagctaaa agtggcggtg tgtttcaggc ggtgctgggg
      241 gcagctgtta tggcggttgc tatatggatg ccgggggtag gaattatggc gagtaatctg
      301 ctgttttctc tcggtgccag tatgacgctt ggcggtgttg cacagatgct ggcaccgaaa
      361 gccagaactc cccgtacaca gacaacggat aacggcaaac agaacacctg tttctcctca
      421 ctggataaca tggttgccca gggcaatgtt ctgcctgttc tgtacggtga aatgcgcgtg
      481 gggtcgcggg tggtatctca ggagatcagc acggcagacg aaggggatgg tggtcaggtt
      541 gtggtgattg gtcgctgatg caaaatgttt catgtgaaac cgcctgcggg cggttttgtc
      601 gtttatggag cgtgaggaat gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg
      661 aaggacaacc tgaaatccac gcagttgctg agtgtgattg atgccatcag cgaagggccg
      721 gttgacggtc cggtggatgg attaaaaagc gtgctgctga acggtacgcc ggtcctggat
      781 agcgagggga agacaaactt ttccggtgtt acggtggtgt tccgcgccgg cgagcaggag
      841 cagacaccgc cggaggggtt tgaatcttcc ggctcagaga ctgtgctggg tacggaagtg
      901 aaatacgaca cgccgatcac ccggaccatc acgtcggcaa acattgatcg tctgcgcctg
      961 accttcggtg tgcaggcact ggtggaaacc acctcaaagg gggaccggaa tccgtcggaa
     1021 gtccgcctgc tggttcagat acagcgtaac ggtggctggg tgacggaaaa agacatcacc
     1081 attaagggca aaaccacttc gcagtatctg gcctcggtgg tggtgggtaa cctgccgccg
     1141 cgcccgttta atatccggat gcgcaggatg acaccggaca gcaccacaga ccagctgcag
     1201 aacaaaacgc tctggtcgtc gtacaccgaa atcatcgatg tgaaacagtg ctacccgaac
     1261 acggcgctgg tcggcgtgca ggtggattca gagcagttcg gtaaccagca ggtgagtcgc
     1321 aattatcatc ttcgcgggcg cattctgcag gtgccgtcga actataaccc gcagacgcgg
     1381 caatacagcg gtatctggga cggaacgctt aagccagcat acagcaacaa catggcctgg
     1441 tgtctgtggg acatgctcac tcatccgcgc tacggcatgg ggaaacgtct tggtgcggca
     1501 gatgtggaca aatgggcgct gtatgtcatc ggccagtact gcgaccagtc agtgccggac
     1561 ggatttggcg gcacggagcc gcgcatcacc tgtaacgctt acctgaccac acagcgtaag
     1621 gcgtgggatg ttctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggg
     1681 cagacgctga cgttcgtgca ggaccgaccg tcggataaag tgtggaccta taaccgcagt
     1741 aatgtggtga tgccggatga tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac
     1801 cgccataatg tcgttgaggt gaactggatt gatccggaca acggtcatga gacggcgaca
     1861 gagcttgtgg aggacacgca ggccattgtc cgttacggtc gtaacgtcac gaagatggat
     1921 gcctttggct gtaccagccg gggacaggcg caccgcgccg ggctgtggct gattaaaacg
     1981 gaactgctgg aaacgcagac cgtggacttc agcgtcggcg cagaagggct tcgccatgta
     2041 ccgggtgatg tcattgaaat ctgcgatgat gattatgccg gtatcagcac cggtggtcgc
     2101 gtactggcgg tgaacagcca gacccggacg ctgacgctcg accgtgaaat cacgctgccg
     2161 tcctccggta ccacgctgat aagcctggtt gacggaaatg gcaatccggt cagcgtggag
     2221 gttcagtccg tcaccgacgg cgtgaaggtg aaagtgagcc gggttcctga cggcgttgca
     2281 gaatacagcg tgtgggggct gaagctgccg acgctgcgcc agcgcctgtt ccgctgcgtg
     2341 agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca tgtgccggaa
     2401 aaagaggcca tcgtggataa cggggcacac tttgacggtg accggcgcgg cacggtgaat
     2461 ggtgtcacgc cgccagcggt gcagcatctg accgccgaag tcaccgcaga cagcggggaa
     2521 tatcaggtgc tggcgcgatg ggacacgccg aaggtggtga agggtgtgag cttcctgctt
     2581 cgcctgaccg tggcagcgga cgacggcagt gagcggctgg tcagcacagc caggacgacg
     2641 gaaaccacat accgcttcag gcagctggcg ctggggaatt acagtctgac agtccgggcg
     2701 gtaaatgccc gggggcagca gggcgatccg gcgtcggtat cgttccggat tgcggcaccg
     2761 gcagcgcctg tcactattga actgataccg gggtattttc agataacggt ggtcccgaaa
     2821 ctggctgtat atgacccgac ggtgcagttt gagttctggt tttcggaaaa gcggattgcg
     2881 gatatcaggc aggttgaaac cagcgcccgc tatcttggca cggcgctgta ctggatagcc
     2941 gccagtatca atatcaggcc gggccatgat tattattttt acgttcgcag tgtgaacacc
     3001 gttggcaaat cggcatttgt ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttat
     3061 ctgagttttt ataaagggtt gatcaataaa acgcatctcg gcaaggagtt gtggacgcag
     3121 attgataacg gtcagcttgc gccggacctg actgaaatca ggacgtccat tacgaatgtc
     3181 agcaatgaaa tcacgcaaac cgtcaataaa aaactggaaa atcagagtgc tgcaatccag
     3241 cagatacaga aagttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag
     3301 ctgcaacaga tgaaggacgg acgcctttat attgcgggta tcggtgccgg tattgagaat
     3361 acgccagcag gaatgcagag tcaggtgctg ctggcggcag acaggattgc gatgattaat
     3421 cctgcgaatg gcaacacaaa gccgatgttt gttggtcagg gcgatcagat attcatgaac
     3481 gacgtgttcc tgaaacgcct gacggctccg accattacca gcggcggtaa tcctccggca
     3541 ttttccctga cacctggcgg acggctgacg gcgaaaaatg ccgatatcag cggtaacgtg
     3601 aacgcgaact ccgggacgct caacaacgtc acgattaata agaactgtcg ggttctggga
     3661 aaattgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa acctttccca
     3721 cgggactccc gggcaccgga gaggtggcca tcagggacca ttaccgtcag ggtttatgac
     3781 gatcagccgt ttgatcggca aattgttatt cccgcggtgg cgtttcgcgg tgctaaacat
     3841 gagcggaaga ataacaatat ttattcgtca tgccgcctga tagtgaagaa aaacggtgct
     3901 gaaatttata accgaacgac cctggataat acgctgatat atacgggtgt tattgatatg
     3961 cctgccggtc acggtcacat gacgctggag ttttctgtat cggcatggct ggtaaatggc
     4021 tggtatccca cagcaagtat cagcgatttg ctggttgttg tgatgaagaa atccacagca
     4081 ggtatcagta tcagctgaat tttataaccc agatacgggc accagaaatg gtgccttttt
     4141 tattgcagaa aagcgagagg taattatgcg taaactttat gctgccattt tgtccgcagc
     4201 catctgtctg gctgtatccg gtgcgcctgc atgggcgtct gaacatcggt ccacgctgag
     4261 cgcggggtat cttcatgcct cgacgaacgc tcccggcagt gatgatctga acgggattaa
     4321 cgtgaaatac cgttatgagt ttacggacac gctggggctg gtgacgtcat tcagctatgc
     4381 caacgctgaa gatgagcaaa aaacgcatta cagtgatacc cgctggcatg aggattccgt
     4441 gcgtaaccgc tggttcagcg tgatggcggg gctgtctgtg cgcgtgaatg aatggttcag
     4501 cgcgtatgcg atggcgggcg tggcttacag ccgtgtgtcg accttctccg gggattatct
     4561 ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg accgggagtg atgacaaccg
     4621 ccacagcaac acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt
     4681 ggccattgac cttgcttatg aaggttccgg cagtggcgac tggcgcactg acggtttcat
     4741 cgtgggtgtc ggttataagt tctgattagc caggtaacac agtgttatga cagcccgctg
     4801 gttcaggcgg gctttttgtg gggtgaatat ggcagtaaag atttcaggtg tactgaaaga
     4861 tggggcgggt aaacctgtcg taaattgtgc gattgaactg cgggccagaa gaaccagtcc
     4921 gaccgttgtg gcacacgttg ttgccacttg cgtgacggac aataacggtg cttatgtgat
     4981 tgaggctgag ccggggtatt acgaggttgc gcttcactgt aacggctggc agccaacccg
     5041 tgtcggggat attgatgtgg caccgactga tgcaccgggg acactgaacg cgtttctgaa
     5101 tgcaccaaag gatggtgatt tacgtccgga ggtgatgaag cgctttgagg aaatggtggc
     5161 gcaggcgcag cagagtgccg gggctgcagc cggaaacgca cagcagacgg cgcaggatgt
     5221 ggcggcagcc gcaacggccc gtgatgatgc acaacgtttt gcggagaaag cccgacagga
     5281 tgcaaccgtc acagctgagg acagaaaggc cactgcggaa gatgtgacaa gcacaggagc
     5341 aaatgcagcc gcagccggac agagcgcaca ggatgccgca ggttatgccc gcgcagcaga
     5401 acaggccaaa aatgacattg atgctgcgct gaccggcact ctgaaaatgg ctaaccatct
     5461 gtc
//
LOCUS       DAJTUM010000180         5400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTUM010000180 DAJTUM010000000
VERSION     DAJTUM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..497
                     /locus_tag="OO471_003955"
     CDS             <1..497
                     /locus_tag="OO471_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6245007.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO471_003956"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO471_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO471_003957"
     CDS             2500..3114
                     /locus_tag="OO471_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6245008.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO471_003958"
     CDS             3114..3443
                     /locus_tag="OO471_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6245009.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO471_003959"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO471_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6245010.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO471_003960"
     CDS             4529..4840
                     /locus_tag="OO471_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6245011.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="OO471_003961"
     CDS             4824..4982
                     /locus_tag="OO471_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6245012.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="OO471_003962"
     CDS             4975..5373
                     /locus_tag="OO471_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6245013.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1178 a         1344 c         1426 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
//
LOCUS       DAJTUM010000181         5370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTUM010000181 DAJTUM010000000
VERSION     DAJTUM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(102..671)
                     /locus_tag="OO471_003963"
     CDS             complement(102..671)
                     /locus_tag="OO471_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR6245014.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(1416..1559)
                     /locus_tag="OO471_003964"
                     /pseudo
     CDS             complement(1416..1559)
                     /locus_tag="OO471_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(1893..2159)
                     /locus_tag="OO471_003965"
     CDS             complement(1893..2159)
                     /locus_tag="OO471_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245015.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            2300..2506
                     /locus_tag="OO471_003966"
     CDS             2300..2506
                     /locus_tag="OO471_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6245016.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            2601..3203
                     /locus_tag="OO471_003967"
     CDS             2601..3203
                     /locus_tag="OO471_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245017.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNINYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            3374..3571
                     /locus_tag="OO471_003968"
     CDS             3374..3571
                     /locus_tag="OO471_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245018.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            4403..5320
                     /locus_tag="OO471_003969"
     CDS             4403..5320
                     /locus_tag="OO471_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245019.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT         1349 a         1374 c         1251 g         1396 t
ORIGIN      
        1 acactgacgt aaaaaacagt cagtgggcga gtggatacat cgttgccgcc agacaacaac
       61 ccatactttt ggtttgccgg atattggcgc gccagtcgtg ctcaactccg gctacagcca
      121 aaattgcgct caccggtgca gtgagctttg gtatgctcct tcgccagata gtcagcacgt
      181 tccagcactt gctgaaagcc ggtgtcatca ttacgcgcca gccacaccgc cggccaggcc
      241 ggaaaatgca ccagcgtggc atggcacccg acatccactc ccagggcact gcaccacgcc
      301 tgtttaatca tcccggccag agactccgga gcgcggtaat caccggcacg acaccaggta
      361 tcccggttga ccagcagcat caggtgatag tgttttttac ctttgcactc cccaaactcc
      421 cgggcccaga cgtaatgcag ggttgtggga tgcacgcgtt taccttcccg atgcttacgt
      481 ttctggtaag cgtcgattcg ggctttcagg gcattgatga agcgggatat cacagctgcg
      541 tccgttgctg ccggtacatc cgggagacgc aggtcaaccc gcaatgccgt caggcgggga
      601 tgaacattca gtgcgtgccg caccgtctca cgaatacgtt gctgccagaa ggggttgtat
      661 ttgtaggtca tggttaaatc tccgtatggt tcatacggaa tagccacgtc gtaaaaaata
      721 cgcagagtcc ctgaagtgac ctccggcagg acactgtctc aggcactctg tgataacccg
      781 aacggtcgtc tccggactga cggttgaatt ttcagcgtcg ttttttaatg taaaaaaccg
      841 gttacgggta aggctgtgag gagggagtga tggtgatacg caaaaagaag tgcagggact
      901 gtggaaacgc gattactcat aatacagtgt gttgcccgta ttgtggttct gtcgacccct
      961 tcggctacta ccggaataca gaccgtatcg ttaccatact gctggcgctg attattgtgg
     1021 tcctgctgac tactgtcagc gtcagtgtgt gtatactgtg tagctggtga gataacggtt
     1081 catgtatccc ctgagctgac agtgtaccgt gtatattaag cgtaaccgac aggttctcct
     1141 tcataccata ttaaattttc cagggattgc cgctgcctta tttcgtccca tacaggaaaa
     1201 ctcatctggg tttctgacaa atacgcgaaa ctcacagccc ctcaatatca accacacgtt
     1261 gtaagccggt aatacctgaa aatcagccta ttaccgccca cgcctccggc accattccct
     1321 tttgatttcg aattctttgt tgtcgctata ccgcttaatg ttcccggtcc tccggtgcag
     1381 ataacaccgg agtcatcgta gcgctgcttc aatattcagg tgacgtattt ccagacggaa
     1441 gccggcgcgc gatcgtaaag tttgttcatt gtcagttctg ccagccggtt gtcaacggca
     1501 gagttaaagg cttccagctc cgcaagggta agtttataac gcgtgtggga attcactttt
     1561 cccgcgtctc ctgacgtatt acgggctgcc acttacctgg tgtcccgcaa tacagcctta
     1621 gtgttccgtt atcggggatc cggacgcacc gcaacaaccg gggaagtatt tctggtttag
     1681 agcagtgtca agagggattt ctcagcccct cactcaccgg ctcagtctca gggcaggagc
     1741 attaatacgc tcagttcatg tcttatacag gaatgggcac agggggagct ggcgtgtcac
     1801 tgctgatttt acgcatcagc gttcatgtat ctgcactctg atacatgaac gattcacagc
     1861 gctctgttac cggagagatt tactctctta gcttactctg gcaaatcctt tacgttactc
     1921 tctgatgact tcttatattt ataataccaa tcagacagcc ttatcccatc ggtgatatca
     1981 gtctcctcct cgccaggaac gggctgactc actgtaacta cctccgaaaa caactctgca
     2041 attatcagaa cgcctgcttc tctccctgtc ctcacgaaaa ctatcccctc tttatcgcgc
     2101 gtgcgtgcgg aagcatcttt tcgcaacaac cacccggtat accggtacgg ctcagccacc
     2161 gcaacctccc cgtttatctc ctggcggtca cattcccgat tattttttac gtttctcccc
     2221 ggttgttatg ccggtgaagg tggcgcgctg ttttcatgac cacaccggct ggtttaacaa
     2281 catccggagg aacattctca tggctacccc agtttcactg atggatgacc agatggtcga
     2341 catggcattt atcactcaac tgaccggcct gacagataag tggttttaca ggctcatcag
     2401 ggatggggcc tttccggctc ccatcaagct gggccgcagc tcccgctggc tgaaaagtga
     2461 agtggaagcc tggctacagg cgcgtattgc acagccccgc ccgtaatttc tgaccgttat
     2521 ccgttcaccc gcagcagacg taatccccgg cctgccggcg gcattctgct gcctgttata
     2581 tccccgtgag gaatatgaaa atgaaacaac agtatcagac ccgctacgaa tggctccacg
     2641 aaagctacca gaaatggctg accggcttca cccggcacgc cgtatcctgg ggcgtgtgtc
     2701 atccgaatat caactacttc cataatctga cgcccggttg ggtgtcattc aatggtgaaa
     2761 aaccggagat tgccatcgta ccccagagcc tgcaccggct gatttatggc cctgacaaac
     2821 gggccacgcc gcccctggat gatgatctga tagtgaattt atgcaccagt gaacatctgc
     2881 tggttcatca tccgatgctg gaaggcattc tcctgtctga atgtgaacgt ctgaggcagc
     2941 gttctctggc aaataaactg atcagcctct tccgtcagtt tggtggcacg gagttgcgcc
     3001 tcaaactggt ctggctttgc tggcttgatt taatgaccgg aaacagcctt gaagactgga
     3061 aagagaacct gaaacggaaa tcagaaaaag agctggagga atggatcatc aaccgtcaga
     3121 ggcagagcgc agcactgacg gatctgatgg atcagtacgt gctcctggca taccgcacaa
     3181 cggttgacga tagccgcaac tgatgccggt tgcttccgga aggtgtgaca aaatacggaa
     3241 atgatgatgc tcccggcccc catgctcacc tgcagagcgt gttcgtcgtc atcatactga
     3301 gccatgacgg gcgtcttaag ggcatcctgt cattgctgag caggcaacac caccagagga
     3361 gccacggaca gggatgttct ttacagcccg atacacgcca ggttctcagc agactgaatg
     3421 tgcaggtgcg gttattcacc agaaatggca tgctgcggta catacccatc ctggtgacaa
     3481 tatgtacgtg gcgacagcga tcccggcact gtggaagaga ctcactcagc ctgcaggata
     3541 ttctttccgg catccgggaa tgtccgcctg aatgcaaaca ggatgatatt tgaatccgta
     3601 atactgttct ttcatcataa ataatttatg cagatacagt taaccgttac agtagccggt
     3661 ttgttttacc gggaaataat atccgtcaga ctgataccgg aacatatctg aaacacgtta
     3721 tgatgttttg ttatcagaaa tgttgtatat tcttatccgt ggcgacagtt taatgccctc
     3781 tggtatcacc tgataatgta tatcctgcga tgattcgtta tatttacggg acacgcctct
     3841 tttcattgtg ctccttttca gacttcattt tccgtaagtg ttccccggca taaaggaggt
     3901 acctttctgg cgtttataac tctctttatc ggtgacgccg gtaatacagg gaataccaca
     3961 ccgacaaacg aggtattaac agacgatatt tcagtgtcca taatttttct gtccggttaa
     4021 ataacgtcag tctgctgccc tctgactgta atcatcaaag ccggaatgca taccgggtta
     4081 caacgtaaaa aaagtgatgc tggcccccgg tgcagctggc gggcgaaaca tgtttacctt
     4141 tcaatacccc ctgtctgcag tctccactct tccatcctct taagctacgt cggccacatg
     4201 agtgcacttc catgataaca ggtattcctg tcagcataac gaagccggtt accttccctt
     4261 cctcctgcat ttcctgtcag aatgactata cagcgcgcag attgcctttt tattgaccat
     4321 cttaatatac cgttataaac ttctttttat catattatta tcaccttatt atgtttaatt
     4381 tttaagcagt cggagaaaga ggatggttca gaaaattttg tcggataagg ttatgaatga
     4441 aagaacaaat gcgtattact cttattatct tggagagcgt aatatctccg tactgcccct
     4501 taatgtttat gatcccccgg aacgttttat cgcttacata aaaaaaaaca gagaaaatct
     4561 gaacatcaca ctttcagatt ttgaacttga acaaataatt tctggtatgc gtctgaaggc
     4621 cctggccttc ctggtccccc tggaaaaaat atcctggatc gcgggcagtg aacgcgcatg
     4681 cttattttcc tggtatctgt tgatgcagtt tatccagaat aacagggcaa aaataagcgc
     4741 agacttgctt caaaagaata aactgtatct taaagaagag tatctggaag ggaatgcctt
     4801 cccgtcggac tcatcgacac agttcagaca aatactcaga gttctcgata ttctttctga
     4861 taagaatttg cgggatgagt ggataatcca gaccaaagac agatggatgc gggcatttaa
     4921 atcaaagagt cctttcagtt acctcctccc ggagaacgaa catgaatgca tatggacatg
     4981 gaattatttg aagggaaaaa atattgcact ggaaaaactg gccagttttc ctggttctgc
     5041 tgatatttac cacgcaattc acctttcatt tgacatatgg gttacctgtc cactcacctc
     5101 acctgacgat ataaaaaatt tcagaaacag tttcaataag gccaaggcgc agcgcaagta
     5161 caaaaaaatg caggaagata aagtcaatgt acagtttttt cttgacgcag aaaccagggc
     5221 gcagctcaaa gaattatcga gggtcagaag actcagtacc ggcgaaatgc tgcatgacct
     5281 gattgttgaa gagtataaaa ggtaccgaca ttccagataa tttcagacat atattatctc
     5341 cgtgagtccc ccgccacctt tccggtgtgc
//
LOCUS       DAJTUM010000182         5343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTUM010000182 DAJTUM010000000
VERSION     DAJTUM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(65..547)
                     /locus_tag="OO471_003970"
     CDS             complement(65..547)
                     /locus_tag="OO471_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245020.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(615..2564)
                     /locus_tag="OO471_003971"
     CDS             complement(615..2564)
                     /locus_tag="OO471_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6245021.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKYFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2649..2984)
                     /locus_tag="OO471_003972"
     CDS             complement(2649..2984)
                     /locus_tag="OO471_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6245022.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2984..3340)
                     /locus_tag="OO471_003973"
     CDS             complement(2984..3340)
                     /locus_tag="OO471_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6245023.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3340..4836)
                     /locus_tag="OO471_003974"
     CDS             complement(3340..4836)
                     /locus_tag="OO471_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6245024.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4833..4994)
                     /locus_tag="OO471_003975"
     CDS             complement(4833..4994)
                     /locus_tag="OO471_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6245025.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5004..>5343)
                     /locus_tag="OO471_003976"
     CDS             complement(5004..>5343)
                     /locus_tag="OO471_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245026.1"
                     /translation="ERDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTL
                     LDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIE
                     HHLEMRFPQN"
BASE COUNT         1272 a         1562 c         1146 g         1363 t
ORIGIN      
        1 taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc tggcggctta
       61 tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag gtcactcccg
      121 actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca tctacaaaaa
      181 actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa tcaaaatcat
      241 catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg tttgcaaaat
      301 caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata atcgcaccgc
      361 tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta tccaagtcaa
      421 cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc tcctcatttg
      481 ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt aaaaatggct
      541 ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct catctttcac
      601 cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt gattttcata
      661 aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc gttgtcaaac
      721 gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag ctgattcctt
      781 tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc accacgtggc
      841 atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca ctcgtcgaac
      901 ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaatattt aacaagccag
      961 ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc accaatcaga
     1021 ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt tgtccattga
     1081 ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat atacagggca
     1141 gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct gacaacctga
     1201 tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt atcaattatg
     1261 agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat attcatcacg
     1321 ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat cgtcagtaat
     1381 gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac aaacgtgcgc
     1441 acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg caggaaggtt
     1501 tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa ctgattttct
     1561 gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg ctgcgcatcc
     1621 gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg cgcaatggcc
     1681 cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc tttcggcact
     1741 tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg catatccgcc
     1801 gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga tttggtggca
     1861 gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc aatttctgac
     1921 tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga ggccacaccc
     1981 gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt cgcacttgca
     2041 ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc tccctgtgtc
     2101 agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc cgtggtgtca
     2161 aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc acgggcgata
     2221 ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat gggcagttct
     2281 gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt atccagacca
     2341 tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga tttgaccggg
     2401 agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga caggctggca
     2461 aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc cgatacctta
     2521 tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca tctcctcgtt
     2581 aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc gtcgcagctc
     2641 cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga ggtgcttccc
     2701 gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat atccagtttt
     2761 gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata tttcagtgcc
     2821 acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa acccagcgcc
     2881 tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc gttatgcgct
     2941 gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga taccctccct
     3001 cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta gcctctccgt
     3061 gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg gtaatcgtca
     3121 tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta aacgtgccct
     3181 taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg ccgacgatgt
     3241 catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat aactgctgac
     3301 catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact cctctgaata
     3361 ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat aatccggcgg
     3421 gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca gatactgttt
     3481 aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg tcgccagaag
     3541 ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac gggtaccatc
     3601 atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt tgcgaagaac
     3661 atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca cgccatacgc
     3721 attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt ccacataagc
     3781 cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc gcttaccttt
     3841 cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag gatcattccg
     3901 gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag gcgactgggt
     3961 ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc cggcaccaac
     4021 cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact ggcgcgcata
     4081 actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg aggcggcatc
     4141 gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa cagcggcggt
     4201 aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa gccctgctgg
     4261 caaaatctca ccaccaccag aaccataata attcagacag accggtaact cattaccata
     4321 cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg ccgtaaacgg
     4381 cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag tggcatcatc
     4441 gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca gggtgccgct
     4501 ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc tggcttccgg
     4561 tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt ccaccatacg
     4621 cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg gcatcagcac
     4681 caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta atgcaggcgc
     4741 gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca acggcaccag
     4801 cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt atcctgttta
     4861 acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc ttccacattt
     4921 cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg ccctttcagg
     4981 ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag atggtgctca
     5041 atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact cagcagggat
     5101 aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt gatttcatac
     5161 ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag cagggtgcca
     5221 ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag ggctttccag
     5281 agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt ttcatcacgc
     5341 tca
//
LOCUS       DAJTUM010000183         5340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTUM010000183 DAJTUM010000000
VERSION     DAJTUM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO471_003977"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO471_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6245027.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO471_003978"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO471_003978"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6245028.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO471_003979"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO471_003979"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6245029.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO471_003980"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO471_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6245030.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1291 c         1458 g         1281 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgacct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAJTUM010000184         5338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTUM010000184 DAJTUM010000000
VERSION     DAJTUM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            109..293
                     /locus_tag="OO471_003981"
                     /pseudo
     CDS             109..293
                     /locus_tag="OO471_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            436..657
                     /locus_tag="OO471_003982"
                     /pseudo
     CDS             436..657
                     /locus_tag="OO471_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="OO471_003983"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="OO471_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1190..2602
                     /gene="ospC1"
                     /locus_tag="OO471_003984"
     CDS             1190..2602
                     /gene="ospC1"
                     /locus_tag="OO471_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6245031.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2706..2856)
                     /locus_tag="OO471_003985"
                     /pseudo
     CDS             complement(2706..2856)
                     /locus_tag="OO471_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2932..4629
                     /gene="ospD3"
                     /locus_tag="OO471_003986"
     CDS             2932..4629
                     /gene="ospD3"
                     /locus_tag="OO471_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6245032.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4746..4865
                     /locus_tag="OO471_003987"
                     /pseudo
     CDS             4746..4865
                     /locus_tag="OO471_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            5032..5304
                     /locus_tag="OO471_003988"
     CDS             5032..5304
                     /locus_tag="OO471_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245033.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT         1799 a         1019 c         1028 g         1492 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      961 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
     1021 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc
     1081 taattacatg cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc
     1141 ttgtagggaa agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc
     1201 agaaacactg aactcagcaa atacccaatg caatatagat tctatggata acagattaca
     1261 tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga
     1321 aaaaaattta aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc
     1381 acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat
     1441 tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc
     1501 gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa
     1561 tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta attctgattt
     1621 gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac
     1681 aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt
     1741 tggcgttgaa atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt
     1801 tgattttggt gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt
     1861 aaccgatcac tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt
     1921 agataaattt tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat
     1981 tatagatgta ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat
     2041 tgactttatt agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct
     2101 tgcccctgtg gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc
     2161 taggatggta agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga
     2221 ggctgttaca gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc
     2281 tctccaggct ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt
     2341 atctaatttt gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat
     2401 tgagtatcta cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa
     2461 gggattggtt gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga
     2521 taacgcaata aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac
     2581 atctgacaat aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc
     2641 acaaaatatc gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt
     2701 ttgatgggtt tcgccccgtt gtagcggtag tcgaactgga aggtcttcag gcactgctta
     2761 cagcgataga gcagcccccc ctgaactgga atggccgttc cggatacccc cctgagtttc
     2821 agagtaacgg tgacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta
     2881 caacaacact aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca
     2941 gtaaatttaa tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc
     3001 tctgttgaga caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag
     3061 aggagttttt tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc
     3121 tgtcgacatt tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat
     3181 tatcatcact ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa
     3241 aaggctatta atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt
     3301 attttttata ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata
     3361 agtacatcaa atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg
     3421 gattttgtca ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt
     3481 aacaaattta acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa
     3541 catcagaaat gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact
     3601 ccaacaagca tgtcctccat aatcagatgg cctgataatt tacttcaccc caaagttatt
     3661 tatcacgcga tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa
     3721 ctatctgacc tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt
     3781 ttgtcaggat tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga
     3841 gaactcttgg aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa
     3901 ggaatgggag gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact
     3961 atcaaaacat acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg
     4021 aaaaatttgc tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg
     4081 tttgctcttc aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc
     4141 ccccctctcc tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat
     4201 gttcccggac ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt
     4261 gatatcttga atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa
     4321 gattctaatg gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt
     4381 attgcttatg ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta
     4441 ctggcagcag aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat
     4501 tttgaggcaa taaaaactta tatggaaata ataaaaaatg aaaatattac acctgaagaa
     4561 atagcagaac acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata
     4621 aaaagctgaa tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac
     4681 cgtctggtct gttacctccg gttccacaaa aataatgtcc atcattttga agtggacact
     4741 atcgtatgga ataccggacc tggatcactg aagctttacg ccttcatttc gaagaacatt
     4801 taccacgggt tgtggccgga cgtcgcctgg gcgtaccaaa atcaactgtc tgtggtatgt
     4861 tcgtgtgaac cgcccccggt atcccgctca tgctggcgaa cccagacacg cagtgaaccg
     4921 ccccgggttt cctggagagt gttttatctg tgaaccacgg cagcctggcg gctgcaaatc
     4981 ggggtgtggc agaatatgag ctttcagagt aaccatccag aatgagttcc catgtcacac
     5041 aacctggagc accagaaagt ccacaccaga atggtgaaag aggttctgaa agccgtcgcc
     5101 agagccaata atcaccctta taagtccgta tttgcagact ttatcaccgg tcatccctcc
     5161 tgcacggtct gtttctggga aaccttccac aaaatgtacc cggattcacc gtacgaatac
     5221 gtcacgttct gccatacctg ccgtcgtttt gatttatacg aaacagaagc ggagatgaag
     5281 gcggatgacc cgaagtggtg gtgattttca gccccgaccg ctgaaacgcc tgttcaca
//
LOCUS       DAJTUM010000185         5244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTUM010000185 DAJTUM010000000
VERSION     DAJTUM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            17..466
                     /locus_tag="OO471_003989"
     CDS             17..466
                     /locus_tag="OO471_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6245034.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(467..700)
                     /locus_tag="OO471_003990"
     CDS             complement(467..700)
                     /locus_tag="OO471_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6245035.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(786..959)
                     /gene="ortT"
                     /locus_tag="OO471_003991"
     CDS             complement(786..959)
                     /gene="ortT"
                     /locus_tag="OO471_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6245036.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1154..1249
                     /gene="yncL"
                     /locus_tag="OO471_003992"
     CDS             1154..1249
                     /gene="yncL"
                     /locus_tag="OO471_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6245037.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1651..2460)
                     /locus_tag="OO471_003993"
     CDS             complement(1651..2460)
                     /locus_tag="OO471_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6245038.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2670..4094)
                     /gene="patD"
                     /locus_tag="OO471_003994"
     CDS             complement(2670..4094)
                     /gene="patD"
                     /locus_tag="OO471_003994"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6245039.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4116..4910)
                     /locus_tag="OO471_003995"
     CDS             complement(4116..4910)
                     /locus_tag="OO471_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6245040.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4900..>5244)
                     /locus_tag="OO471_003996"
     CDS             complement(4900..>5244)
                     /locus_tag="OO471_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6245041.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1395 a         1350 c         1129 g         1370 t
ORIGIN      
        1 aaccggacgc gattggatga atcagtcgct cacccttgct tttttgattg ccgccggaat
       61 tggtctggta gtccagaaca cgctgatggc gcgcatcacc cagacctcct ctaccattct
      121 catcgccatg ttgctgaact cactggtggg gattgtgctc tttgtttcta ttttatggtt
      181 taaacaaggc atggcggggt ttggcgaact ggtgtccagc gtgcgctggt ggacacttat
      241 acctggcttg ctgggttcgt tttttgtctt cgccagtatc agcggatatc agaatgtggg
      301 agcggcgaca actattgcgg tactggtcgc cagccagctg attggcggtc tggtgctgga
      361 tatcttccgc agtcatggcg taccactacg ggcgttgttc ggcccaatat gtggcgcaat
      421 tttacttgtg gttggcgcct ggttggtggc cagacgctca ttttaatcag aggatggtgc
      481 cgcctttggt taactgttcg tggcgcgctt ccatatcttc tttatgcttg cgaccgtgat
      541 gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg atcttcccga cttaactccg
      601 cgtcatcgct cagggcgatt aataattcgt tcatatgggc aatgacgctc tcttcaatcg
      661 cggcatccac gcgcgcgatg acttcatcca gatgcgacat actttctcct tagccacttg
      721 tgaaatttat cgattaatcc aaatgatata agtctgcgtg gtgccgtcaa gccagtgagt
      781 aagatttaaa acagtgaaaa caacagcgcc accgggaaac tcatcggcca tgtggccccc
      841 accagaaagg cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa ccaggtgata
      901 agaaatgcga ttgctgccat aaccgcataa aaaacaagca tatgttgata gagaaacatg
      961 tacgggcgtc cagccatatg aataaaacgc gcgcaaatat gcgccatttt ttgacgtgca
     1021 tcaagttttt tcaccagttt gagaaaaaat catcaaacag caacttgccc ttatccatct
     1081 tcagaatgag tattattgcg gattttctta acccgtacta tacacatagg ggcaatgata
     1141 aaaggtggca aaaatgaatg tttccagtag aactgtagta ctgataaatg tctttgctgc
     1201 tgttggtttg tttactctta tctctatgag atttggctgg tttatttgat gtagtgattt
     1261 tgcacgcagc acgtactgtc agagaaatca actctctgac agtacgcaag atcttctcat
     1321 actctccaag tttgcaacat tatccttaat atttacttct ttttgctggc atctacattg
     1381 aaatgagtag aaaacatccc cctactgtcg tctatacaaa cagcttactg attcaaacgg
     1441 aatcttgttt tcacattttt ctatcacgaa tactttatac gcgcgggagt ctataaatgc
     1501 tttatacttt caaggaataa cgtaaatata aaaagatggc gtaaagtata tgcatggcag
     1561 gatgtttgca acctccttga tgacgctgcc ccattgaaat tgcaaacccc gccctggtta
     1621 tcttgcccat ttatcatgca aaacagataa ttataggcct ctgaatacct cagaaagatt
     1681 gaatttttta tagtccctct cttgaatatt tccatttgat atcaatcgat tcatctcttc
     1741 aacaaaataa tttatatcat cacacttatt attatccgga agatctgagt agttataatt
     1801 ccaccatttt attctcaaca gttcatcaat aacatttgaa tcaaacctaa atctgattat
     1861 ttttgcagga actcctgcaa ctatggcata tgggggcaca tctttggtaa caacggaatt
     1921 ggcagcaata acagcaccat cacctattgc gatgccacct ttcaatacaa catcatttcc
     1981 tatccagaca tcatttccta taatcggatt ttcaggtttg gtaatgaatg gtttcaatgt
     2041 ccagctacgt tcaaattcac tcatagcaaa cttatcaaat tcaccgttat aagtcatcat
     2101 gtgagtagta aaacgattca aaggatgatt tccacccatt atttttacat ttgatgcaat
     2161 actacaaaat cgacctatac gtatattccc tgaaaaatgg caggtcggaa atgaaaaagc
     2221 gccgacactg gagaaatttt tcacaggcat tgttgaatat tcttcagcaa taacattatc
     2281 ttcaattgtt atcttgtcac cttttgagaa aagaaataaa ttcctttctt tatacgactt
     2341 cttcccctta tgatttaaaa agattttatt atccatacaa aactcaaagt gttttttcgt
     2401 ccatctgaag gtaagggttt tatttttatc ttccgtgaat ttgtacaatt gaactttcat
     2461 tgttaatact tctacttata aagtattagt ttaataacga tactctaaag ccttatgcta
     2521 atcaactgtg acaggatgaa aaatcgccat caaacattta gcctaatttt tacgccagtt
     2581 attaatgtaa tcattctaat acttaaccgc cattaaactc agaacctaaa acgatagtgg
     2641 ccgtcctata acagccacta acatctaact taatgtttaa ccatgacgtg gcggacgacg
     2701 gtgtaatcct ccagcccata aagtgacata tccttgccgt aaccagaaag tttctgccca
     2761 ccgtgcggca tttcacttac cagcatgaaa tgggtattga cccaggtaca accatattgc
     2821 agccgtgcgc tgacgcgatg cgccctgccc acatctttcg tccataccga agatgcaagt
     2881 ccgtactggc tgtcattcgc ccagttcacc acctgttctt cgttgtcgaa gggcgtaaca
     2941 ctcactactg gaccaaatac ctctttttgc acgatggcat cgtcctgtaa tgcgccagcc
     3001 agcagcgtcg gcgcatagta atagccatta cccttgcgct tttcaccgcc agtgatcact
     3061 ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc tgacgcgttc gagatgcgcc
     3121 agcgagctta aaggtccaag ctccgtagac tcgtcatccg gcgcaccaga ttttaacgtt
     3181 gccaccgcag cacccagttt ttccaccagc gtatcgtaaa tgcctttttg cgcgtagatc
     3241 cgacaagccg cagtacaatc ctgtccagca ttgtaatagc caaatgtacg tacaccttcg
     3301 accactgctt caatatccgc atcatcaaaa acaatcactg gcgctttgcc accaagttcc
     3361 atatgagtac gcttaatgga cgacgcggta tggctgatga tgtgctcgcc ggtggcgata
     3421 gagcccgtca gcgacaccat ccgcactttg ggatgaccgg tcagcggatc acccaccgtt
     3481 ttgcctctgc caaacagtat gttaatcacg cctgccggga agatatcttt cgccagctct
     3541 gccaacttca acgcggtcag cggggtaatt tctgatggtt taagcactac gcagttccct
     3601 gccgccagcg ccggagcaag tttccacgcg gccatcatca gcggataatt ccacggtgcg
     3661 atagaagcca cgacccccaa cggatcgcga cggatcatcg aagtatgacc ttcaagatat
     3721 tcacctgccg ccagaccatt cagacagcgc gccgcacccg cgaaaaagcg aaaaacatcg
     3781 acaatcgccg ggatttcatc attgaacgca ctatgcagcg gtttgccaca attacgggat
     3841 tccagttcgg caaaaacctg accattttct tcgataacat ccgccagttt cagcaggcat
     3901 tccgcacgcg ctttcggtgt ggtttgcccc cattcagcaa atgctgcatc tgccgcacgc
     3961 acagcagcat cgacctgctc tgcggatacc tcggcaatct ccagtaaaac gtcccccgtt
     4021 gccggattat agacaggctg tttttcccct tcgccgctaa cccgttctcc gttaatcagt
     4081 aacttatgtt gcatagctta ttcctgtatc agtggttatt gaccattgtc gccttcgcgg
     4141 gttagccacc aggcccccag gatcggcaag gttgttacca acataaccag cagtgccacc
     4201 acgttggtta ccggtacatc acgcggtcgc ccaagctgat tgagcaacca caacggtaac
     4261 gttcgttcat gacctgccgt aaaggtcgta acgatgattt catcgaacga caaggcaaac
     4321 gccagcattc ctcctgccag taacgccgaa ctgagattcg gcaacactac gtagcggaag
     4381 gtttgccagc cattggcccc aagatccatt gacgcctcaa ccagactcca cgaggtgcgg
     4441 cgaaaacggg cgatgacatt gttaaacacc accactacgc aaaaagtcgc atgaccgacc
     4501 acgatggtga aaaatcccgg ctccagattg atggttttaa aggcggttaa tagcgccaga
     4561 ccagtgacaa tgcccggcag cgcaatgggc agcagcaata acagcgaaat ggcgtttttg
     4621 ccgaaaaagt ctcgtctcca cagcgcggca gctgccagcg tccctaacac cagcgcaatt
     4681 aatgtcgcca gcgccgccac tttaagtgac agcgtcacgg catcaagaat atcactacgc
     4741 tgtgctgcca cgctaaacca gcgcagcgtc aggccctgcg gtggaaaact aaacgccgca
     4801 tcttcagtgt taaacgcata ggcggcgatt atcaggatgg gaaaatgtag gaaaacaacg
     4861 ccgccccagg ccgccagttt gaggaaaaac ggtgcgcgtt cagagtgcat cgaacgctcc
     4921 cagacgtttc acgaacgcca ggtacagtgc gatgagaata atcggcacca gggtgaatgc
     4981 cgccgccatc ggcatattgc caatcgcccc ctgctgggaa taaaccatat tgccgataaa
     5041 atatcctgga ggaccaacca gctgcgggac gataaaatcg cccagtgtga gtgagaaggt
     5101 aaagatagag ccagcggcaa tacccgggat tgccagcggc agcaccacat agcgaaaggt
     5161 ttgtcgtgga cgtgcgccga gatcagccga cgcctgcaac aatgacggcg gcaaacgctc
     5221 aagcgccgcc tgaacgggca ggat
//
LOCUS       DAJTUM010000186         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTUM010000186 DAJTUM010000000
VERSION     DAJTUM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..220
                     /locus_tag="OO471_003997"
     CDS             <1..220
                     /locus_tag="OO471_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6245042.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="OO471_003998"
     CDS             complement(392..721)
                     /locus_tag="OO471_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6245043.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="OO471_003999"
     CDS             complement(726..911)
                     /locus_tag="OO471_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6245044.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="OO471_004000"
     CDS             complement(908..3547)
                     /locus_tag="OO471_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6245045.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="OO471_004001"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="OO471_004001"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6245046.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO471_004002"
     CDS             4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO471_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6245047.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1307 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aa
//
LOCUS       DAJTUM010000187         5140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTUM010000187 DAJTUM010000000
VERSION     DAJTUM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            180..437
                     /locus_tag="OO471_004003"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO471_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO471_004004"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO471_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6245048.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO471_004005"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO471_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6245049.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO471_004006"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO471_004006"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6245050.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO471_004007"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO471_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..4410)
                     /locus_tag="OO471_004008"
     CDS             complement(3595..4410)
                     /locus_tag="OO471_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6245051.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4507..>5140
                     /locus_tag="OO471_004009"
     CDS             4507..>5140
                     /locus_tag="OO471_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6245052.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT         1372 a         1173 c         1225 g         1370 t
ORIGIN      
        1 cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3781 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3841 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     3901 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     3961 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     4021 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     4081 taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact
     4141 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     4201 cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc
     4261 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
     4321 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
     4381 tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca
     4441 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
     4501 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
     4561 actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat
     4621 gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct
     4681 gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat
     4741 gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc
     4801 aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt
     4861 gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat
     4921 gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac
     4981 taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa
     5041 cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag
     5101 gccgctttga ctcaggcact actggatctg gaatataccc
//
LOCUS       DAJTUM010000188         5106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTUM010000188 DAJTUM010000000
VERSION     DAJTUM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(329..574)
                     /locus_tag="OO471_004010"
     CDS             complement(329..574)
                     /locus_tag="OO471_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245053.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1351..2193)
                     /locus_tag="OO471_004011"
     CDS             complement(1351..2193)
                     /locus_tag="OO471_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6245054.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2278..2475)
                     /locus_tag="OO471_004012"
     CDS             complement(2278..2475)
                     /locus_tag="OO471_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245055.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2495..2983)
                     /locus_tag="OO471_004013"
     CDS             complement(2495..2983)
                     /locus_tag="OO471_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245056.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2980..3357)
                     /locus_tag="OO471_004014"
     CDS             complement(2980..3357)
                     /locus_tag="OO471_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6245057.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3404..3781)
                     /gene="cbtA"
                     /locus_tag="OO471_004015"
     CDS             complement(3404..3781)
                     /gene="cbtA"
                     /locus_tag="OO471_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR6245058.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3861..4082)
                     /locus_tag="OO471_004016"
     CDS             complement(3861..4082)
                     /locus_tag="OO471_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6245059.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4169..4645)
                     /locus_tag="OO471_004017"
     CDS             complement(4169..4645)
                     /locus_tag="OO471_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6245060.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4661..>5106)
                     /locus_tag="OO471_004018"
     CDS             complement(4661..>5106)
                     /locus_tag="OO471_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6245061.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1190 a         1241 c         1352 g         1323 t
ORIGIN      
        1 gtccccagaa aagaagacag gcatagagtt ttgtgatcta ctgattgcgc taacaaattc
       61 aatgagatca cctctatgcc tgcccgttaa gtatgtcagg cttttttccg taatgctcta
      121 acttccacgc attggtttct gacatcaaat aaggctatga ctttctctcc cttttaatct
      181 cattctgatc taaaaccata tcttaaaaaa atgcatgggt attttaatta ttttgatgtt
      241 gatctaacgc actcaaggac cattttatta ccgattatcc attctattta actgttgcga
      301 tataaatgtt tgttttaggt gctttaattc agtacaagtc atcaaaccgt accagaccct
      361 gactgtcagt gacaccgaca ggtgggggcg gcggtgtggg gtcaaactga gtgtgtctgc
      421 ctctggccca cagagccagc tctgccgcca ttgcccgtgg atgatactcc agctgcacgt
      481 tgagaccgga aactgggttg ttgttctcat ccacgaactg cagcgccagc cagtgcatcc
      541 gctgcttacc gcaggcgact tctttgtact tcattttgtg actcaggcag gtgactgtcg
      601 tgttgcctgt tgcgtccatt ttgcggttaa acccttttaa ttcaatttgt taaattgctt
      661 tccacctttt gcggggcgcg acttaaccaa aatattcact gttatcataa tatgcaatgg
      721 gctggaggtt gcaggtcatt tagggatttt ctgtaagccc cgttttgcag aacgttctat
      781 ctccgattga gatcctgtct ctcggtatta ccggtagtct ggccataatc ccctatggat
      841 catattgata tagtaggcat aacacccaaa agttgactaa agctaaatca atcagagtga
      901 aaaatgttcg gtttgatgtt gggggggact gaggaggggc caacgcaggt ggtatgcgtt
      961 ttacttcaga ccggacaaag accggatgta tccgtgcagt aatgaatccg ttagccaggt
     1021 gctcatgtgg aaatagtgtc tattggggat ttgaagtttg acagaaaagg ccacagtgac
     1081 cagaaggggg ctacttagaa gaaatgttca ggctgggtgg aagaggtaaa gcagtgacat
     1141 cagcattcaa agtgaccgga tatctaccag aaccccagaa agcaaaaaac cagcccgtag
     1201 gctggttctt ctaaatagtg gtgccggact cggaatcgaa aatttctttt atcatattga
     1261 tattaatgca atttgtttgg tttgattaaa aatgtaccct caattatacc cgcagtccct
     1321 tttgatatcg aaagggaatc actctcggga tcaggctgag agcacagaag gaaagtacct
     1381 ggctattata tcgttaattc tgtcaatcag ttccagcctt ttgaaagtga tgtgtgcaga
     1441 tcccttctta aagtatttaa tgatgaacat ctcgtcttca tagcgctctt taccctgaac
     1501 ggactggatg tgaccatcca gacgaatagt gatgttttcc cggttgtcgg ggatcggttt
     1561 gccgctgaac agatgtagca ttctttccag gtcgacgagt cggtcagtct gttgcccggt
     1621 gataagatga aacccccacc ggtcccacct caccaggttg ttgacgataa ttttactgcc
     1681 aaatttacag ggtgagttgg ttttgtaatc ccagctcagc cctttgaaga cgttgatcac
     1741 tccccgttca aacacttcat ctttgttctg gtgcagttgt tcaaatgtgc tcagaatgtt
     1801 cgcttcgctg atttccggaa aattatcata ttccagtgac ctgtaccatg tgtcccgggt
     1861 ctgcgcatcc atcagactca gcatcccgga acgttgcatc agatcacgcc agagttcgcg
     1921 atcaagattg cgtgtgatgg ctttcattgc ggtttccggt ttctccatca gccagcaacc
     1981 gcagcgaaaa tcctgtttca tggcccagtc cagagccgtt ttacctctaa tactttctgt
     2041 cattgctgat acttcagcca gttgtcggat aagtgtgtcg atttgcagca atgtactctt
     2101 tcgtacagcc agaatgtggc ggatactggt tgagcagata acgtccgtat gcccgttgac
     2161 cacttccgga cgagaggctg tgatttcaga cataatcgat attccttttt gcagataaac
     2221 gaaaacgcca gccgtattca ggctggcgtc agtggaatga agtctggtga gtaagactca
     2281 ccggttctgt tgcagaaact gcagctgacg cagggtacgg atatcttccc tggcctgttc
     2341 aatacagggc agcagtgctg ccgaatccgt tttatcctca tcgttgtcga agataatccc
     2401 tgattcgcag tcaatattgt cctgcagcca cgcaatcaga atatccagcg ccgtttctgt
     2461 ggttaataat ttcatccagt gtcttttgtc ctgattacca gtcgaaagtg gggaaccccg
     2521 gcagacattc tgcgccggcg tccagatgaa tgagactgac gccataacgc cgggtgagag
     2581 tgacgaccag acgacggaac gttctggaca ggcccaggcg tttaagccgc agaaccgggt
     2641 acgaccatgc atcggtacgt aacagatagc cgctgcctgt aaaatgaatc cattctgttt
     2701 cgccgtcgtc attagtctgg tgtgacagcg agtacagcca ggcgttgtcc gtttccgtga
     2761 tatgtgcggt gctgcaacgt atgcaggtaa gggttgttaa cggtggtggt gtactggttg
     2821 cgtgttggtc ggatttatcg actacgcgga gtgaataacc gttttcagag accttgttaa
     2881 tcagttcact gagattaaca ccatcgacgt caacgacaat gcgccccatg ttcagtgcct
     2941 gcacgttaac gctgtcggct tccagcgtca gggccagttt cattgtttcg cttccggatg
     3001 cttcccccgg gtaatgttat ttaccgttct gtagttatcg cgggtcatca ggccggttgc
     3061 ccggcgtgcc cggagaatat caatgctgtt gattaactgc gacggggctc ctgcgctgaa
     3121 tcccggctgg tcggtacgta ccagcgcgta tttttcaacg agaaagttca ccgcgtcaca
     3181 cagtgaaatt cctgcctcaa tatgctgctc aatcacacgt tcatcagcga acggtgtgtc
     3241 attcagcgtg aggccatagt gctggtccag cagtcgggtg agcagtgtct gccagatggt
     3301 gatgggagac gggcagcccg atgcctcccg tacgtgagtg tcgggtaatg tattcatctt
     3361 aagttctctg taacaggtgg ttataaaggg gagatatccg gtgttatacg gtggttgcgg
     3421 gcgctgccgg tgtcggataa acagccagat aaacgtaacc acaggagccg agggtgtcgg
     3481 cttcgcaggt cagccctttc gcatacagtg tgacggtatg ctgatggcgg ggattcagtt
     3541 caccgccagt gagcatgagt tccagttgtt tcatcagcag cggaaaggcc tggtccaggt
     3601 ggtacgcatc tacgtcgctg aacctgcctc tgataccggc gcggtctgca aggtaatgca
     3661 ggcggttacc ctcctgtacc agtcgggccc cgaaacaggg cgtcacggtg cagggcagcc
     3721 cccaccaggg gcggtcgtga ttgtcgtcgg gaggcgttgt cccggggagt gtgtctgaca
     3781 cgataaagtc ctcacagaaa atcggttaag aatggtccgg tagtggcgat aattctgctc
     3841 atcagaattc ccttccagac tcagttcagg gtgacgctca tcagccggac atacggacca
     3901 aaactgtcct tacggcgttc tgcaaacaca gccagtacac cgggaatatc ctgcacttca
     3961 cggccggtat atgtatccgt gctaccgtgc cagcggtatt taccggtaca gaacggaaaa
     4021 agacgggatg caggatgctg acggtgaata cgcatggctt caccacgggt gatgattttc
     4081 atgagaatag tcctcctgaa tgtgaaagta agaaacagct gagaaagaga aactgaagtg
     4141 atatgaaggt gattgtgacg gtgacgggtt aaagcagacc gtgttccgca aaggagaaaa
     4201 cctggctgcc accgactatc agatggtccg gtacccggat atcaaccagg gccagtgcct
     4261 gtaccagacg ctcagtgata aggcggtctg ccttgctggg ggggacttca ccggacgggt
     4321 gattgtgcgc cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc
     4381 ggggatggac ttccgtacgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct
     4441 gattctggtt gttcagatac agcacccgga actcttcacg ctccagtccc gccatgttca
     4501 gaatcagcca ttcccgtgcc gcatgcgtgg aggtgaaggc cacgccgggt tcatgaagat
     4561 ggcggtccag ggttttcagg gcccgcagaa tgaggctgcg ctctctggtt gtcatttctc
     4621 cgggcagaaa ggaaagctgc tgcattgtcc ctgccaccat tcagtcgatg atgcgcataa
     4681 tggcgctgca ttccggatgc tgcagggcgt aatcccgcag ccggtaataa tggaccgtca
     4741 tggcataaca ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatgc
     4801 cggcagcttc cggactcatt tcagcgcggt taccgttcat ggcattgaac agtacccatg
     4861 tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt
     4921 acagattcca gataccaccg cagtagtctt cgcacagacg gtccatccag ccgaagacac
     4981 ggggctccag ggtcacccac tgtggaatga ggccaaagtg ctgcggccag aagctgatgc
     5041 gctgttcatc agggacgagg gtggcaacca gttgttgcag gtcagtctcc ggtgcacagg
     5101 ccgaag
//
LOCUS       DAJTUM010000189         5083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTUM010000189 DAJTUM010000000
VERSION     DAJTUM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..655
                     /locus_tag="OO471_004019"
     CDS             <1..655
                     /locus_tag="OO471_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6245062.1"
                     /translation="AGSLQSYKGSLYWNNVTYPFPLTTNTNVLDIGDKTPMPLPLKLY
                     ITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNIISNNSVVMPTGGCTVDS
                     RNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVG
                     VTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            989..3268
                     /gene="ydeP"
                     /locus_tag="OO471_004020"
     CDS             989..3268
                     /gene="ydeP"
                     /locus_tag="OO471_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6245063.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3516..3719
                     /gene="safA"
                     /locus_tag="OO471_004021"
     CDS             3516..3719
                     /gene="safA"
                     /locus_tag="OO471_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6245064.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3787..4548
                     /gene="ydeO"
                     /locus_tag="OO471_004022"
     CDS             3787..4548
                     /gene="ydeO"
                     /locus_tag="OO471_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6245065.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4950..>5083
                     /locus_tag="OO471_004023"
     CDS             4950..>5083
                     /locus_tag="OO471_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6245066.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     S"
BASE COUNT         1454 a         1083 c         1133 g         1413 t
ORIGIN      
        1 cgcaggctcc ctacagtcat ataaaggatc gctgtactgg aataatgtga cctacccctt
       61 cccgttgacg acaaatacca acgtattgga tattggtgat aagacaccga tgccattacc
      121 gttaaaactc tatattaccc cagttggggc ggccggtgga gttgtcataa aagcggggga
      181 agtcattgcc cgtattcata tgtataaaat tgcaacatta ggaagcggaa atccgcgtaa
      241 ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg gctgcaccgt
      301 tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag aaatacccct
      361 tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg ccaccactga
      421 cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat cgggagtggg
      481 tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct ccctggggac
      541 agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa caggaaacaa
      601 ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg aataattgtg
      661 attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt attttgtgaa
      721 ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag tcttattttt
      781 tatattggaa aatatgttaa tttaaacact atgagagatg atgcttaact tatcgtattt
      841 aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct tactcttata
      901 tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt acaaatccta
      961 ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct accagggtgc
     1021 tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc agatggatat
     1081 acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg actgtccggg
     1141 ttgtgcatgg ccagatccta agaacagtgc gtcattcgac atttgtgaaa acggcgcaaa
     1201 agcaatcgct tgggaagtca cggataagca ggtaaatgcc tctttctttg ctgagaatac
     1261 ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc gtctcactca
     1321 gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc aacaagcttt
     1381 cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg aattctatac
     1441 ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc gtgaatacgg
     1501 gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg tgggtttggc
     1561 agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga agtgcgattt
     1621 agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga cttcgttgcg
     1681 cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg aacgtggcct
     1741 ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg agactcagtt
     1801 ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca aggggatgat
     1861 gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct cattgcttga
     1921 tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg acgttctcaa
     1981 ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa tcgccgaact
     2041 ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg ggatcactca
     2101 gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga tgaaaggtaa
     2161 cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg tacagggcga
     2221 ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg
     2281 ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca tgcaagcaat
     2341 atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac tggcaatgcc
     2401 agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac acgtagccac
     2461 taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc cggttctggg
     2521 gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat
     2581 gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga
     2641 gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg tagcctggga
     2701 gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc tgccagagtt
     2761 cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa atgcagctgc
     2821 tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca aagggctgtt
     2881 agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac gcagccacga
     2941 tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat tcggtcaacg
     3001 agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag
     3061 agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca tggatagatt
     3121 aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc cagaatcgaa
     3181 tcacatgcta acacttgata accacgatcc attaagtggt attcctggct ataaaagtat
     3241 tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc tgtcatccgc
     3301 acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg attaaattaa
     3361 actacatact ataagatata aacttctgcc tacagctgta agaaactccg ctcagtactg
     3421 aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac tgattaacga
     3481 cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca gtgaaaaaca
     3541 aaatcacgca aagagataac tataaagaaa tcatgtctgt aattgtggtt gtcttattac
     3601 tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt tcagaaatgg
     3661 gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac tggaaataaa
     3721 cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt gttttaagga
     3781 gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa cgctaacatt
     3841 ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa cgctgttcgt
     3901 acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga aaataccatt
     3961 aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc tgcgaagttt
     4021 ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta tctcaactcg
     4081 gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt
     4141 gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt ttccgggaaa
     4201 gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac
     4261 tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc
     4321 tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt
     4381 tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt tatttatgcg
     4441 ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagg
     4501 cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt atttgctaac
     4561 gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc ctgccccgcg
     4621 ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca tatttatgca
     4681 cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt cagtaaaatg
     4741 caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta cttaatagaa
     4801 cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct aaatctgcta
     4861 tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa attacttcgg
     4921 aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa agtgtcgtaa
     4981 gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct catgcggcag
     5041 atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctc
//
LOCUS       DAJTUM010000190         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTUM010000190 DAJTUM010000000
VERSION     DAJTUM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO471_004024"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO471_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6245067.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO471_004025"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO471_004025"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6245068.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO471_004026"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO471_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6245069.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO471_004027"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO471_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6245070.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO471_004028"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO471_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6245071.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTUM010000191         4836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTUM010000191 DAJTUM010000000
VERSION     DAJTUM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(80..664)
                     /gene="kptA"
                     /locus_tag="OO471_004029"
     CDS             complement(80..664)
                     /gene="kptA"
                     /locus_tag="OO471_004029"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6245072.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            914..1597
                     /locus_tag="OO471_004030"
     CDS             914..1597
                     /locus_tag="OO471_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6245073.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1594..2055
                     /locus_tag="OO471_004031"
     CDS             1594..2055
                     /locus_tag="OO471_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6245074.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2068..3240
                     /gene="iadA"
                     /locus_tag="OO471_004032"
     CDS             2068..3240
                     /gene="iadA"
                     /locus_tag="OO471_004032"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6245075.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3305..4216
                     /gene="hypT"
                     /locus_tag="OO471_004033"
                     /gene_synonym="qseD"
     CDS             3305..4216
                     /gene="hypT"
                     /locus_tag="OO471_004033"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6245076.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4209..4601)
                     /gene="iraD"
                     /locus_tag="OO471_004034"
     CDS             complement(4209..4601)
                     /gene="iraD"
                     /locus_tag="OO471_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6245077.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4598..4681)
                     /gene="idlP"
                     /locus_tag="OO471_004035"
     CDS             complement(4598..4681)
                     /gene="idlP"
                     /locus_tag="OO471_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6245078.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1139 a         1272 c         1213 g         1212 t
ORIGIN      
        1 cttcagactg ataacaaacg ctacattgcc ggatgtgctg cgcttaccag gcctatataa
       61 cccctgcaat atattgaatt tattgttttt gtaccccagt cggcataaac gaccactcaa
      121 gaaactcaac agcaaccgtt gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga
      181 tgcctcgttt cgccatttcc tgtgctttga cggttaagac aaccggcgat ccgtgtcttg
      241 ctcccacttt gcgtgccgtt gcttcatcag cagagagatg cacgtaatga cgatcccctg
      301 caatcagccc ctgttttttt atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa
      361 actgtggcgg tgttttttcg gcaaaggaaa tagcgacctg cgaagtcgaa tgcccctgaa
      421 ccgcgcggat gcacctacca tcactggaat aactaaaacg ctttttatcg ctggttgcga
      481 ccacggtatc cagcaatgca cgggttaatc gcttgcccgc tttttgggca caaagtatga
      541 gcttattgat atccgcccat ccttcacggt ccagtacgat accaatcgcc tccggtttgt
      601 gtcggaggac aaaacttaaa aacttgctcg tatccgccag ctctttttcg ttgtactttg
      661 ccatcgcgtt ttcgtccctg gattttctgt ttttatttac taatgtcatt gtcactgggt
      721 ttatgaattt tctgcaactc aatcctgcgc agaacctttt ccacacagcg taatttttcc
      781 cctatgcaaa aaacgcatag ccagaaacat acttgcattt tccccatacc cacatggctg
      841 ataaggtttt tcagttccag gaattttcct ggctcaagca acacacaaac acaaaaaaac
      901 aatggggatt gttatgacac aacaagggga tgctgtagcc ggggagctgg cgacagaaaa
      961 agtgggcatt aaagggtatc tggctttttt tctcaccata atcttttttc ccggtgtttt
     1021 ttccggaaca gacagctggt ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg
     1081 gcaactgcct ggtgcgaatg gagccaccac ctcatttcgc ggggtgggtg gcgcgggggc
     1141 taaagatggc tttctctttg cgctggagct ggcaccgtca gtcattctct ctctggggat
     1201 tatttcaatt accgatggtc ttggcggatt acgcgccgcg cagcagttaa tgacacctgt
     1261 gctaaaaccg ttgttgggca ttccagggat ctgctcgctg gcactgatcg ctaacctgca
     1321 aaacacggat gccgccgccg gtatgaccaa agagctggcg caggaaggtg aaattaccga
     1381 acgcgataaa gtgattttcg ccgcatatca aaccagcggt agcgccatca tcaccaacta
     1441 cttctcttcc ggcgtcgcgg tgtttgcctt tctgggcacg tccgtaatcg tacctttagc
     1501 cgtcatcctg gtgtttaaat ttgtcggagc caacattttg cgcgtctggc ttaacttcga
     1561 agaacgtcgc aacccaacgc aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc
     1621 atggatatgt ttatcgatgg cgcgcgccgt ggctttacta tcgccacgac caacctgctg
     1681 ccaaacgtgg tgatggcgtt tgtcatcatt caggcgctga aaattaccgg cctgctcgac
     1741 tgggtggggc atatctgtga acccgtaatg gcgctgtggg gattacccgg tgaagcggca
     1801 accgtccttt tagccgcatt aatgagtatg ggcggcgcag tcggtgttgc cgccagtctg
     1861 gcaaccgctg acgcattaac cggccacgat gtcaccgttt tactgcccgc aatgtattta
     1921 atgggcaacc cggtgcaaaa tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa
     1981 tattacccgc atatcattac ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg
     2041 cagcttattg tttaacaagg agttaccatg attgattata ccgcagccgg ttttaccctg
     2101 ctgcagggag cgcatttgta tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct
     2161 aacggcaaaa ttatcgccgt tgccagcaat atcccttctg acattgtacc gaactgcacg
     2221 gttgtcgatc tcagcgggca gatcctctgc ccaggtttta ttgatcaaca cgtccatttg
     2281 attggcggtg gcggcgaagc aggtcccacg acgcgcacgc cggaagtggc gctaagtcgc
     2341 ctgacggaag cgggcgtcac gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc
     2401 cacccggaat ccctgctcgc caagacccgt gcgctcaatg aagaaggcat cagcgcctgg
     2461 atgctgaccg gcgcttatca tgtcccttcc cgcactatta cgggttccgt ggaaaaagac
     2521 gtggcgatta tcgatcgtgt gattggcgtg aaatgcgcca tctctgatca ccgttctgcc
     2581 gcaccggacg tttatcacct ggctaatatg gcggcagaat cccgcgttgg cggtttgctc
     2641 ggcggtaaac ctggcgtcac cgtgttccac atgggcgaca gtaaaaaggc gttaaagcct
     2701 atttatgacc tgctggaaaa ctgcgatgtg ccgatcagca agctgctgcc gacccacgtt
     2761 aaccgcaacg taccgttgtt tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc
     2821 gatatcacca gcagcattga cgaaccggtc gcccctgccg aaggtattgc ccgcgccgtt
     2881 caggcgggta ttccgctggc acgcgtcacc ctcagctccg acggcaacgg tagccagccg
     2941 ttcttcgatg acgaagggaa tttaacccat atcggtgttg caggttttga aacgttgctg
     3001 gaaaccgtgc aggtgctggt caaagactat gatttcagta tcagcgatgc cctgcgcccg
     3061 ctcaccagta gcgtagccgg tttccttaac atgagcggga aaggcgaaat tctgccaggc
     3121 aatgacgcag acttactggt catgacgcca gaattgcgca ttgagcaggt atacgctcgc
     3181 ggcaaactga tggtcaaaga cggcaaagcc tgcgtgaaag gaacgtttga aacggcttaa
     3241 acggttgtca gccgccctaa tggacgtaga atgcccccaa gggcggctga cagagtaaaa
     3301 cgtaatggat gactgtggtg cggttttgca taatattgaa accaaatggc tttatgattt
     3361 tctgactctg gaaaaatgcc gcaatttttc ccaggcggca gtcagtcgca acgtctcgca
     3421 accggcattc agccgccgca tccgtgcact ggaacaggcg attggcgttg aattgtttaa
     3481 ccgccaggtg acgccactgc aactctcaga acaagggaaa atcttccatt cgcagatccg
     3541 ccatctgttg caacagttag aaagcaacct tgcagagctg cgtggcggca gcgattacgc
     3601 gcaacgtaaa atcaagatag ccgctgcaca ctctctttcc ctcggtctgt taccgtccat
     3661 tatcagccag atgccgccgc tctttacctg ggcaattgaa gccattgatg tcgatgaagc
     3721 ggtcgataaa ctgcgtgaag ggcaaagtga ctgtattttt tcctttcacg acgaagattt
     3781 gctggaagcg ccgtttgacc acattcgctt atttgaatct caattgttcc ctgtctgcgc
     3841 cagtgacgaa cacggagaag cactttttaa cctcgcgcag ccacactttc cgttactgaa
     3901 ttacagccgc aactcctaca tggtgcgatt gattaatcgc accctgacgc gccacagtga
     3961 gttaagtttc agcacctttt ttgtctcttc gatgagcgag cttttaaagc aggttgccct
     4021 cgacggctgt gggattgcct ggctgccgga gtatgccatc caacaagaaa ttcgcagcgg
     4081 gcagctcgtt gtgctgaatc gggacgaaca ggtgatcccg attcaggcct acgcataccg
     4141 gatgaatacc cgcatgaatc cagttgccga acgcttctgg cgtgaactgc gcgagctgga
     4201 gattgtgctt agctgacatt ctcaagcgtc gcactgcgcc aaccgccatt atcccagacc
     4261 agatcaaacg ttagcggttc atcgtcgtgg tacagcatta tctcaaggct gatcgccaga
     4321 gtgtggcagt acgcttcttt caaattaacc tgcaacgccg caatccgtgg ctcatgaaat
     4381 aacagtgcag aacgcagctg acggcaatac cagctctgga gatgtttgtc gctcgggtta
     4441 tgcacttgcc actcgggcat gcctgaaaca agtaatgacg gtaatgccga atgaggagaa
     4501 ttaaaaagag tgaggagatc gctgcatacc gatttacgca gcaatgacgt tttattgcct
     4561 gaaatttcag gtaaaacagc ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt
     4621 tagcaacata tgcggcttgc cctgaacacc gggcaccact gtattgatgc tcattttcca
     4681 tagccattcc cttttgttca ttacatccat gtcagtctga gacaggcctg aatcctgtca
     4741 gcaaagtata aaaccttact tgcctatgaa tatctactga cttttcagga aaaaagtcat
     4801 gatttgataa aaatcatcaa actcaaaaaa aaaacg
//
LOCUS       DAJTUM010000192         4776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTUM010000192 DAJTUM010000000
VERSION     DAJTUM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..447
                     /locus_tag="OO471_004036"
     CDS             <1..447
                     /locus_tag="OO471_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6245079.1"
                     /translation="IEDQSTNCGENARFSITLLNQAVERVHTAIVVQDPTMQRRTMAT
                     FRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPRLH
                     DDSDGYGPRGRDFIVHVDFPAEVIHAWQTPKHDAVLIEAMESRSLR"
     gene            644..2083
                     /gene="aldA"
                     /locus_tag="OO471_004037"
     CDS             644..2083
                     /gene="aldA"
                     /locus_tag="OO471_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6245080.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2125..3125)
                     /gene="gap"
                     /locus_tag="OO471_004038"
                     /pseudo
     CDS             complement(2125..3125)
                     /gene="gap"
                     /locus_tag="OO471_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3314..3844
                     /gene="cybB"
                     /locus_tag="OO471_004039"
     CDS             3314..3844
                     /gene="cybB"
                     /locus_tag="OO471_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6245081.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4089..4262
                     /locus_tag="OO471_004040"
     CDS             4089..4262
                     /locus_tag="OO471_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245082.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4334..4483)
                     /gene="hokB"
                     /locus_tag="OO471_004041"
     CDS             complement(4334..4483)
                     /gene="hokB"
                     /locus_tag="OO471_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6245083.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4584..4724)
                     /locus_tag="OO471_004042"
                     /pseudo
     CDS             complement(4584..4724)
                     /locus_tag="OO471_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1221 a         1098 c         1187 g         1270 t
ORIGIN      
        1 attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct ttagcatcac gctattgaat
       61 caggccgtag aacgagttca tacggctatc gttgtacagg accccaccat gcagcggcgc
      121 acgatggcaa cgttccgccg tatgaccggg gacaatcccg atgcaccacg ctggttaagt
      181 tatcccggat tcgttcctca gttaggaaat aacgcagaca gtgtaatctt tattaatcag
      241 ttacaaggat tatggccggt tgagcgttat ctctcactac tcactggcga gctgccgcgt
      301 ttacacgatg atagcgatgg ctacggtccc cgcgggcgag attttatcgt tcacgttgat
      361 tttccggcag aagtcatcca tgcatggcaa acgccgaaac atgatgcggt gctcatcgag
      421 gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa
      481 cgggcatgac tcctgacttt tatttctgcc ttttattcct tttacacttg tttttatgaa
      541 gccctccaca gacttgtcct ttcccgatgc cgtctctctg atgattgatg ttaattaaca
      601 atatattcac cgaaaacaaa catataaatc acaagagtcg cccatgtcag tacccgttca
      661 acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat ggattgatgt
      721 ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg ccgaggatgc
      781 ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat
      841 tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc cgcgaacgcg ccagtgaaat
      901 cagtgcgctg attgttgaag aagggggcaa gatccagcag ctagctgaag tcgaagtggc
      961 ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg agggcgagat
     1021 tattcaaagc gatcgtccgg gagaaaatat tcttctgttt aaacgtgcgc ttggtgtgac
     1081 taccggcatt ctgccgtgga actttccgtt cttcctcatt gcccgcaaaa tggctcccgc
     1141 tcttttgacc ggtaatacca tcgtcattaa acccagtgaa tttacgccaa acaatgcgat
     1201 tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc ggcgtgttta accttgtact
     1261 ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg caatggtcag
     1321 tatgacaggc agcgtctctg caggtgagaa gatcatggcg actgcggcga aaaacatcac
     1381 caaagtgtgt ctggaattgg gtggtaaagc accggctatc gtaatggacg atgccgatct
     1441 tgaactggca gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc aagtgtgtaa
     1501 ctgtgcagaa cgtgtttatg tacagaaagg catttatgat cagttcgtca atctgctggg
     1561 tgaagcgttg caggcggttc aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg
     1621 gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga
     1681 agaaggggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat attattatcc
     1741 gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg aaacctttgg
     1801 cccggtgctg ccagttgtcg catttgacac gctggaagat gctatctcaa tggctaatga
     1861 cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg cgatgaaagc
     1921 cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg aagctatgca
     1981 aggtttccac gccggatggc gtaaatccgg tattggcggc gcagatggta aacatggcct
     2041 gcatgaatat ctgcagaccc aggtggttta tttacagtct taatgagtga aagaggcgga
     2101 ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg tgcgaataag
     2161 ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca gttgtaaatc
     2221 gcccacggcg gtaatttccg tttgcgtggc atcaaacact gaaccgaaat ggctgccaat
     2281 gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat tggtggttgc
     2341 ttgtttaagt gcgttattca cctcttcggc agtcactttt ttccgagaat ggacaccagc
     2401 tcagtgaccg aacctgtttt caccggcacg cgttgcgcat gacctttcag tttgccgctc
     2461 agttccggga tcaccagacc aatggctttt gccgcccccg tagtgtgggg aatgatattt
     2521 tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac cagtgactgg
     2581 gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa actgtcatgc
     2641 aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg acgccacgga aacaatggtg
     2701 tcgttgccat ccagagtgtc gtcattaacg ttataaacga tggttttcat ttcaccggca
     2761 ggagcggaaa tcaacacctt cttcgcacca gcatcaagat gcgcctgcga tttatcggcg
     2821 gaggtataaa agccagtaca ttcgacaatg atttctgccc ctttcgcttt ccacggaata
     2881 tttttagcct ctttttcggc gtaaaccgcg atacttttcc catcaacgat aagtgaatct
     2941 tccgtaaaat caacgctcca ggggaacgga ccatagtttg aatcatgttt cagcagatag
     3001 gcgagaattt ttggggaagt aagatcatta atagcgacaa cgtctatgtt gcttttgact
     3061 tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa aaccgttaat accaacttta
     3121 ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc aggcttagac
     3181 caggattgtg ggatgggcaa tctccattct tacagtgaaa cgtaacgtac tgaaaacggg
     3241 tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg ttaacggaaa attatgttaa
     3301 agcaggaaat gttatggaaa ataaatactc aaggttacaa atcagcattc actggctggt
     3361 ctttttgctg gttatcgcag cgtattgcgc aatggagttt cgtggtttct tcccacgtag
     3421 cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc atctcaattc tcgtgctgat
     3481 ggtggttcgt cttttgttaa gactgaaata cccaaccccg ccgattatac ctaagccgaa
     3541 accgatgatg acgggactgg cgcatttggg acatttggtg atttatctcc tgtttattgc
     3601 gctgccagtg attggtttgg tgatgatgta taaccggggc aacccgtggt ttgcgtttgg
     3661 tttgacgatg ccttacgctt cagaggccaa tttcgaacgg gttgatagct taaagtcgtg
     3721 gcatgagacg ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg cggctgcact
     3781 ggcacaccac tatttctgga aggacaacac acttctacgc atgatgccgc gtaaacgttc
     3841 ctgaaggata tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat
     3901 acgggtctac atcggaagcg cctattatat ttatttgtat gacaaataaa accataatcc
     3961 ttgcccatac gtccatctgg cttattttta atcaattcac ccgatctttg atctcatcaa
     4021 cggtatcaaa ataaaccagc gtataacgtt caaatcgcac ataagattcg ataaaccatg
     4081 gaggttatat gaaaaaactg gcacttattt tgtttatggg aacgcttgtt tccttttatg
     4141 ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac tgtacggcag
     4201 gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca tgcactaact
     4261 gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag tcagatgcgc ggggaaaatc
     4321 cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc agcaacctcc ttatcaccgt
     4381 cccggaaccg cagttcgtag agcgtttgtc gggtcaggag tgtgaatgcc agaatcgtaa
     4441 tgcagataat gagcagacac accaccagag ggttgcgctt catagccttt ctccttgccg
     4501 gatggcgggt aagaggctaa caatctgaat tgcttggttc attcgttggc ctcggtttgt
     4561 actgacccca aaaagttgga cagttaaaca cgaggcatat aggtctgatt ccgatattca
     4621 tttggagtca gaccttttaa tttcaggcta attcttctgc tgttgtagta ttcaatatat
     4681 tccgtaacag catccttcag ttcgcttata ttactgaact cattagactg gccccctgaa
     4741 tctccagaca accagtatca cttatttaag tgatag
//
LOCUS       DAJTUM010000193         4727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTUM010000193 DAJTUM010000000
VERSION     DAJTUM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..287
                     /locus_tag="OO471_004043"
     CDS             <1..287
                     /locus_tag="OO471_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6245084.1"
                     /translation="EWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVK
                     LSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(488..1345)
                     /gene="sitD"
                     /locus_tag="OO471_004044"
     CDS             complement(488..1345)
                     /gene="sitD"
                     /locus_tag="OO471_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6245085.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1342..2199)
                     /gene="sitC"
                     /locus_tag="OO471_004045"
     CDS             complement(1342..2199)
                     /gene="sitC"
                     /locus_tag="OO471_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6245086.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2196..3023)
                     /gene="sitB"
                     /locus_tag="OO471_004046"
     CDS             complement(2196..3023)
                     /gene="sitB"
                     /locus_tag="OO471_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6245087.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3023..3937)
                     /gene="sitA"
                     /locus_tag="OO471_004047"
     CDS             complement(3023..3937)
                     /gene="sitA"
                     /locus_tag="OO471_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6245088.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4369..>4727)
                     /locus_tag="OO471_004048"
     CDS             complement(4369..>4727)
                     /locus_tag="OO471_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6245089.1"
                     /translation="HRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLG
                     TRWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWI
                     DDMNDEQIAMLNARLS"
BASE COUNT         1263 a         1147 c         1141 g         1176 t
ORIGIN      
        1 ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc ctgtccccgg
       61 tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc gatgccgata
      121 atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca atggtgcagg
      181 cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag gaagagctga
      241 atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag aagctgcggc
      301 acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg cgagtattga
      361 atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc gtacctcctc
      421 ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc tgtcctcgga
      481 tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt gtgcgtgcgt
      541 tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg acaatcgtcg
      601 gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac actattaccg
      661 atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt actgcaatgg
      721 cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc agcgtcgcca
      781 caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta tgcaaaccgc
      841 ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat tttacgctaa
      901 taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg atggttatcc
      961 cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt gcaatataca
     1021 agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta tcttgcttga
     1081 ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg gcgacgaatg
     1141 cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga aaaaccgcat
     1201 gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc gacagcagcg
     1261 cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag gtgaactgca
     1321 gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg cacgccgacg
     1381 atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca gcagtgtctg
     1441 cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc acgcgccgag
     1501 gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa aacgatcggt
     1561 taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa aggcaccaac
     1621 cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga tttttaatct
     1681 gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca ccataagatc
     1741 tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga gttgcaggat
     1801 atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt tcacggatgt
     1861 cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga tcaggccgat
     1921 gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc ccgctgccag
     1981 tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg cccccgcaac
     2041 acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt ttagcatcag
     2101 atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca tcgcattgag
     2161 catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct ctgcaccgct
     2221 gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc ttccgagccg
     2281 ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc ggctgtaaaa
     2341 gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt ataatcgcaa
     2401 aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc ttccgcgcgt
     2461 aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc ggtaaaaggt
     2521 tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa gacgcgtttc
     2581 ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat atcgacacgt
     2641 tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa catacccata
     2701 tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga ccagtcaacc
     2761 tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg cgtgggaata
     2821 cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc tttaaacaac
     2881 gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact tggtactgtg
     2941 aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac atcattaacg
     3001 acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt ccctgtacca
     3061 gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg ttttctgtgc
     3121 tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc gcaacctgac
     3181 gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga tttttcttaa
     3241 ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca ttaatcggcc
     3301 acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc ccttcactgg
     3361 tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg ggggcaaggg
     3421 tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt gcatttgccg
     3481 gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca ttatctggcg
     3541 acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc cctactggcg
     3601 tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa aagcgttgga
     3661 accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc gcacgtttaa
     3721 tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt atggatgaga
     3781 cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta aatgttgtaa
     3841 tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg agggcgaggc
     3901 accccaagag catggttact ttttttattg agtgcataat ttagtaccta tagtgagcaa
     3961 aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa aatgaaaata
     4021 attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg ctggcgacat
     4081 gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat gaggaaaatt
     4141 gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt tgtcgctgat
     4201 aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt gtgatttttg
     4261 cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt cttgcattat
     4321 ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact acgataaccg
     4381 cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac cgtaaatttc
     4441 atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg ggttcgctcc
     4501 tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac ggcggcgaat
     4561 accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg agtaataagg
     4621 tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt gtttttcagt
     4681 tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgat
//
LOCUS       DAJTUM010000194         4636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTUM010000194 DAJTUM010000000
VERSION     DAJTUM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..311
                     /locus_tag="OO471_004049"
     CDS             <1..311
                     /locus_tag="OO471_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6245090.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO471_004050"
     CDS             468..1217
                     /locus_tag="OO471_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6245091.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO471_004051"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO471_004051"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6245092.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO471_004052"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO471_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6245093.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO471_004053"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO471_004053"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6245094.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="OO471_004054"
     CDS             4084..4557
                     /locus_tag="OO471_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6245095.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1137 g         1098 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAJTUM010000195         4631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTUM010000195 DAJTUM010000000
VERSION     DAJTUM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..370
                     /gene="bglB"
                     /locus_tag="OO471_004055"
     CDS             <1..370
                     /gene="bglB"
                     /locus_tag="OO471_004055"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6245096.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            456..2072
                     /gene="bglH"
                     /locus_tag="OO471_004056"
     CDS             456..2072
                     /gene="bglH"
                     /locus_tag="OO471_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6245097.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2099..3274
                     /locus_tag="OO471_004057"
     CDS             2099..3274
                     /locus_tag="OO471_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6245098.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3276..3998
                     /locus_tag="OO471_004058"
     CDS             3276..3998
                     /locus_tag="OO471_004058"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6245099.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4060..>4631)
                     /locus_tag="OO471_004059"
     CDS             complement(4060..>4631)
                     /locus_tag="OO471_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6245100.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1284 a         1026 c         1131 g         1190 t
ORIGIN      
        1 acgggttctg ttaaatacgc tttgggatcg ttatcaaaaa ccgttattta ttgtcgagaa
       61 tggattaggc gtaaaagaca gcgttgaagc ggatggttcg atacaggacg attatcgaat
      121 tgcctattta aacgatcacc tggtacaggt aaatgaagcg attgccgatg gtgtggatat
      181 tatggggtac accagttggg ggccaattga tttagtcagt gcatctcatt cacaaatgtc
      241 taagcgctac ggctttattt atgtggatcg tgatgataat ggcgaaggaa gcctcacaag
      301 aacgcgtaag aaaagcttcg gatggtatgc agaagtgatc aaaacgcggg ggctgtcatt
      361 aaaaaaataa ccaataaagc accttaatta tcgtcgcatt cagaacagtc tggatgcgat
      421 acgttaattc tttctttgca ccataaaggg atattatgtt tagacaaaat cttattacct
      481 ctgccatctt attaatggca ccgttagcct tttctgcaca atcattggct gaatcattaa
      541 cggtggaaca acgccttgag ttattagaaa aagcgttaag agaaacgcaa agcgaactca
      601 aaaagtataa agatgaagag aagaaaaaat atacgccagc gacggtgaat cgtagcgtaa
      661 gtacgaatga tcaagggtat gccgccaatc cgttcccgac cagtagtgcc gcaaaacctg
      721 atgctgtact ggtcaaaaat gaagagaaaa atgccagtga gacaggctcg atttattctt
      781 ccatgactct gaaagatttc agtaagtttg tgaaagatga aattggcttt agttacaacg
      841 gctattaccg ttctggttgg gggaccgcct ctcatggttc acctaaatca tgggcgattg
      901 gttctctggg ccgctttggt aacgaatact ccggctggtt tgatttgcag ttaaaacaac
      961 gtgtctacaa cgaaaacggc aaacgggttg atgccgttgt gatgatagat ggtaacgttg
     1021 gtcagcagta ctctaccggc tggtttggcg ataatgccgg tggcgagaac tttatgcagt
     1081 tctccgatat gtacgttacc accaaaggtt tcctgccctt tgcgccagag gctgatttct
     1141 gggtgggtaa acacggtgcg ccgaaaattg aaatccagat gctggactgg aaaacgcagc
     1201 gtactgatgc tgcagcgggt gtaggtctgg aaaactggaa agtcggtccg ggtaaaattg
     1261 atatcgcgct ggttcgcgaa gatgtcgatg attacgatcg cagcctgcaa aacaaacagc
     1321 agattaatac ccatactatt gatttacgct ataaagatat cccgttatgg gataaagcga
     1381 ccttaatggt gagtggtcgt tatgtcacgg caaacgaaag cgcatcggaa aaagataatc
     1441 aggataataa cgggtattat gactggaaag atacctggat gttcggcaca tctttaacgc
     1501 agaaatttga taaaggtggc ttcaacgaat tctccttcct ggttgcgaat aactctatcg
     1561 ccaggaactt tggccgttat gctggcgcaa gtccatttac cacctttaat ggtcgttatt
     1621 atggtgatca caccggcgga acagcagttc gtctgacctc gcagggcgaa gcctatatcg
     1681 gcgatcattt tattgtggct aacgcgattg tttactcctt cggtaacaat atttatagct
     1741 acgaaacagg cgcacactct gatttcgaat ctattcgtgc ggttgttcgc ccggcctata
     1801 tttgggacca atataaccag acaggtgttg aactgggcta tttcacccag caaaacaaag
     1861 atgcgaatag taataaattt aatgagtctg gttataaaac cacgctcttc cataccttta
     1921 aagtcaatac cagtatgttg acctcgcgtc tggaaattcg tttctacgcc acgtatatca
     1981 aagccctgga aaacgaactg gatggcttca ccttcgaaga caataaagac gcccagtttg
     2041 ctgtcggtgc ccaggctgaa atctggtggt aatggaactt aatgaaagga atattacgat
     2101 gaatataaaa attgctgctt taacgctggc tattgccagc ggtatttctg ctcagtgggc
     2161 catagcagcg gatatgccag ccagcccggc acccactatc ccggtgaaac agtatgtgac
     2221 tcaggtcaat gccgataaca gcgtgacctt tcgctacttt gcccctgggg caaaaaatgt
     2281 ctctgtagtg gtgggtgttc cggttccgga caatattcac ccgatgacca aagacgaagc
     2341 aggagtctgg tcgtggcgca cacctatcct gaaaggcaac ctgtacgagt attttttcaa
     2401 tgttgatggt gtacgcagca ttgatacagg caccgcaatg actaagcctc agcgccaggt
     2461 taactccagt atgattctgg tgccaggcag ttatctggat acgcgttctg ttgcgcatgg
     2521 tgatttgatc gccataactt accactccaa cgcattgcaa tctgaacgtc agatgtatgt
     2581 ctggaccccg ccaggataca ccggcatggg cgagcctttg ccagtgctct atttctatca
     2641 cggttttggt gataccggac gttccgctat cgatcagggg cgtatcccgc aaatcatgga
     2701 taacctgctg gctgaaggga aaattaaacc gatgctggtg gtgatcccgg ataccgaaac
     2761 cgatgcgaag ggcattattc ccgaagattt cgtgcctcag gaaagacgta aagtctttta
     2821 tccgctgaat gctaaagcgg cagatcgcga actgatgaac gatattatcc cgctgattag
     2881 caagcgtttt agtgtccgta aagatgccga tggtcgcgcg ctggcagggc tttcacaagg
     2941 cgggtaccag gcgctggttt ccggaatgaa tcatctggaa agctttggct ggctggccac
     3001 attcagtggt gttaccacga caaccgtacc ggatgaaggt gtcgcggccc ggctgaacga
     3061 tccggcagct attaaccagc aactacgtaa ttttactgtg gtcgttggtg ataaagatgt
     3121 cgtaaccggc aaggatatcg ccgggctgaa aactgagctt gagcagaaaa aaattaagtt
     3181 tgattaccag gaatatcccg gcctgaacca tgaaatggat gtctggcggc ctgcctatgc
     3241 agccttcgta cagaaattta aaataaggca ttaagatgaa attaatcatt accgaagatt
     3301 accaggaaat gagccgtgtc gcggcacacc atctgcttgg ttatatgtcg aagacgcgtc
     3361 gtgttaacct ggcaattacc gccggtagca cgcccaaagg catgtatgaa tacctcacca
     3421 ccctggttaa aggtaagcct tggtacgata actgctattt ctataatttc gatgaaattc
     3481 cctttcgcgg caaagaggga gaaggcgtaa cgattaccaa tctgcgtaat ctgtttttca
     3541 cccctgcggg gatcaaagaa gagaatatcc agaagctcac tattgataac taccgcgagc
     3601 atgatcagaa actggcgcgt gaaggcggcc tggatttggt ggtgctggga ttaggtgcag
     3661 atggtcattt ttgtggcaat ttgccgaata cgacccattt ccatgagcaa accgtggagt
     3721 tcccgatcca gggagagatg gttgatattg tggcgcatgg tgagttgggc ggcgactttt
     3781 cgttagtgcc tgacagctac gtgacgatgg ggccgaaaag catcatggcg gcgaaaaact
     3841 tgctgataat tgtcagtggc gcaggtaaag cgcaggcact gaaaaatgtg ctgcaaggtc
     3901 cggttaccga agatgttcca gcttccgtgt tgcagctgca tccttcgctg atggtaatcg
     3961 ctgataaagc cgctgccgca gaattagcgt tcggttaagc tgaagaaata aggccggatg
     4021 tatgaattac gctccggcct gttctcatta tttaaataac tacgaaaaat caccccacag
     4081 tctcagcttg ccgcagtgga gacagcggaa gagataaccc tgacaatcac cacccttttg
     4141 caggcattta gctagatcag aatttctaat accgaaattc tcacaatctt cttcaaggtt
     4201 ggcaaattca tcgaggcgat cttttatatc attccagccc acatagccga taaaagcaca
     4261 gaaatcgcca caatgcgcaa gccagaattc ctgttgccat ccatgataac ctggcgtgcg
     4321 ttcaaccaac tctttcagca tttcatcagg atatgtgttc ttaataccgg caaattcgtc
     4381 ctcttcatca tactcaaatt caacaccttc tatgctggca tcatcctgaa aactacctgc
     4441 aaacttttct gcagcagaac cgtccgcaat acacaacgga cagagatgtt caacttcatc
     4501 aacgcaataa aagggacccg agtaataaac tgacgtctgt tgttcacagc aatcgcactc
     4561 tacggtttta tcctgttcaa atgcgcctgt ttccagtggc ttgggatgat atttgaatgt
     4621 aagcgccccg a
//
LOCUS       DAJTUM010000196         4614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTUM010000196 DAJTUM010000000
VERSION     DAJTUM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..285
                     /locus_tag="OO471_004060"
     CDS             <1..285
                     /locus_tag="OO471_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245101.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO471_004061"
     CDS             296..1036
                     /locus_tag="OO471_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6245102.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO471_004062"
     CDS             1095..1481
                     /locus_tag="OO471_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6245103.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO471_004063"
     CDS             1490..1819
                     /locus_tag="OO471_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6245104.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..>4614
                     /locus_tag="OO471_004064"
     CDS             1791..>4614
                     /locus_tag="OO471_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6245105.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRR"
BASE COUNT         1138 a         1065 c         1485 g          926 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcg
//
LOCUS       DAJTUM010000197         4562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTUM010000197 DAJTUM010000000
VERSION     DAJTUM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..546)
                     /locus_tag="OO471_004065"
     CDS             complement(<1..546)
                     /locus_tag="OO471_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245106.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2197)
                     /locus_tag="OO471_004066"
     CDS             complement(1208..2197)
                     /locus_tag="OO471_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245107.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2281..2772)
                     /locus_tag="OO471_004067"
     CDS             complement(2281..2772)
                     /locus_tag="OO471_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245108.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(3070..3816)
                     /locus_tag="OO471_004068"
     CDS             complement(3070..3816)
                     /locus_tag="OO471_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6245109.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         1148 a         1080 c         1135 g         1199 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gagttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa gacaggtgaa
      841 tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac ttgaagcacg
      901 taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt ttggtctggc
      961 tcaggtcagg ctatgggggc ttttggctcg ctctttatat tagatgaggt ctcacatttt
     1021 tcagtcaaaa ctcatcgtct aatagcccat agccagacca ccagtcccag ctttagcagg
     1081 aagaccacga gatagacttt gtgtaggtcc atttggccct cctactttct gtattcacaa
     1141 actagattac tagtacaaaa taaaggccag acatccagtt cggaagtctg accaatagtc
     1201 gttattgtta gttagcttct ttagtgaagc tcaggcgttt gactacaggc aggacagcag
     1261 tagccgagcc agtacccatc ttgatggtcg tagcagcttc agctacaaca aacaccttgc
     1321 cgagaatctc aaggtcgtga gactgggagt ctccatcacg tgaaaggacc acagtaatgc
     1381 tctcgccatc gtgacggaag caaccgaagc tcagagccgt aaaacgctta gccatcatga
     1441 acacgccacg gtcttcaccg ttgtaatagg cgacgtcagt ccccagcata tcgacgatag
     1501 cgtcttcacc accgaagccc tgcttagcag ccagacggga gagagccttg aggtgtgact
     1561 catgggcgat aatgccgtag tcagaaacct cagaaccgaa agaagactcc gtgttctcgt
     1621 acagggcatc catgatagcg tcagccttag cggtcaggct accagtcagg gccggaagtt
     1681 ctacggtctt cagcagcttt gaggactcca gcacgtcaac catcttagag ttaatggtac
     1741 gaatcacgtt atcgcagatg ttcagtgcgt cctgctggag cgtcccctga gtgaaaccag
     1801 tggtcgactt ctggtcccaa agctttgttt ggtgagacac gaggatcgcc tgttcaatcg
     1861 gctcaacggt gatttcagtg ccagacggag cggtcacaac ttcaggtcgc ttaccaccac
     1921 ggacagggac cagcacagag cggtcagcca gagtgtgagc aatgattgga gtcggtaagc
     1981 tgttacgggt cggcaggact tctacaggga acgggatgtt atcttgagcg tctttaagta
     2041 caccgttcag catagcctta acagtcttag ctaactggag ggcatagacc tgagggtcgg
     2101 cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct
     2161 caatgccaaa ctggaaagcg tttttagtca tattcattat aattaatctc cttgagtcaa
     2221 atacttgaag tccctaacag gcagtcaggg accgtgtgtt ctcagatgag ggacgcttgc
     2281 ttacaagctg tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacgtga
     2341 gcgaccagcg gaatgagtag cgctgcggag gttttcacca taggtccggc gttcagcttt
     2401 gacctgttcg cggcgctgta gctctttttg ccattcgtct tcggtcatga acatttcgcg
     2461 ggtacgctct ttctgagacg ctagctcttt agcgatacgc tcagcttcag ctcgtttgtt
     2521 catctggcgg cgttggtagg cggtcatcag gtcattactg aagaagttac caatggtcac
     2581 gccagcttca gccagagctt ccagtaactc catcgcctcg cggctggtgg acggtaagcc
     2641 tgacaggtag cccggaatga gggtcttgat agcgagcgac aacggatagg actgaccgtc
     2701 cagactcaca gtgttttcat aaaggttgat gtcggtgact gacagcttcc agttgttgtt
     2761 gttgaacatc ataggtaatt ctcctcttga gagttgagac tatcggtatc gcaggcacag
     2821 taaggcctac gggtctcccc gcatcggtca tctcaaaagg tgtttaaatt aggtattgag
     2881 taggtgagct actaacggtt aagcggcagt ttcatagcga ccagttgggc tattagctcg
     2941 tctcgggatt ggtgctggcg ggtctcatct tccgggtcta gaccaagctc tctgaggagg
     3001 acttcagtcc ggtagccacg gtaacggaga gtcatgcgcc cctcaggtgt gcgcgggtca
     3061 atcatcatct tagattccct ccaccttacc ggattgtgcc agcttcagga ggtgctcttg
     3121 cttctgctgg tcggtgccag tgaatgcaat aaggatgcct cgcatcttgt caaccatctc
     3181 aggcttaccg aagacctcca gccccttagc gttgaaccgg aagttatacc cacctgctga
     3241 gaacttctgg tctgacggct taatgatacg aaagcagtcg gaatacttcg gatttacaga
     3301 ccactcgtta ctgattgggt tcttacgaaa gaggccacga tgctggagaa gtgcggtcat
     3361 tagcttctga gcgggcagac cataggtttt gcagtagtcg ccccaagtca cgcctcggcg
     3421 gttagcggac tgaatggctg ctcgcttctt ctggtcttcc agttgaagga ctcgacgctc
     3481 taacgtctga atatggtcgt aacacttggc gcggaggtga acggaatagc cagcgaccag
     3541 aatgaaagta tgcttacggt cgagacgata cattgttcgg ctctcacctt tcgcgtcctt
     3601 gtatacttcc tcgccaacga gcccaaattt ggacccgtta agctcgctgc tcaaagttga
     3661 gcagtgctca gttttggccc cgttaagctc ttcgaccata ttacgaatat ctcgtaaaac
     3721 gtgtttatgt tcttttcctg tcagctcagc aatttcaact gaggtcatgg acacaagctg
     3781 gtcgaggtta atgatgctga tttcttgttg ggacatttac gtgtactcct tttgtttgca
     3841 ccctgaagtc gcaccccact gacctactca agcccccacg taaagggaga ccagcaggca
     3901 acaggacgca aacagaatga cttgaggtaa ttggggttag tggtctgcga tgaccataga
     3961 gatgaaggtt cgacagcatg tccgagcgtg tgtggtgata gacttaagtc acgagaccaa
     4021 agaacacaca ggctgaccag aggctgtctt gccttaggct ggtggctagc ttgctagcgt
     4081 ggaccacggt ttctcgcttg ccgtctctca ggaggtcaga ggcgagatat ggtccctata
     4141 gtgatagttt agtcaaacag gaatgaactt cggagaactt gacgcgaaag tgttcagtac
     4201 ttccagtaag acccgaagca tggagaccac atcatagcga ccataatttt acttcgggtt
     4261 ggcgggagtg gaggctggac ttaaagttct tcttattgga attgtgataa cacaatccat
     4321 aacaagataa actcatggtc tcctccagcc tgctggtcaa gactcaaagc tccaacctca
     4381 gacaccactg atctaacctc tttgagtttc cgcctgtcca caaatggatg gcagacctca
     4441 gtaatggtgt cttggttctt cagttatccg cgcagcgatt atcacagagc caacttaaag
     4501 tcggatgtgg tctactgctc cccgcctgtc aaaagggaag atagcttacg ttaaaaggaa
     4561 tg
//
LOCUS       DAJTUM010000198         4561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTUM010000198 DAJTUM010000000
VERSION     DAJTUM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(37..825)
                     /gene="phoH"
                     /locus_tag="OO471_004069"
     CDS             complement(37..825)
                     /gene="phoH"
                     /locus_tag="OO471_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6245110.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1432..2697)
                     /gene="efeB"
                     /locus_tag="OO471_004070"
     CDS             complement(1432..2697)
                     /gene="efeB"
                     /locus_tag="OO471_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6245111.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2703..3830)
                     /gene="efeO"
                     /locus_tag="OO471_004071"
     CDS             complement(2703..3830)
                     /gene="efeO"
                     /locus_tag="OO471_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6245112.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3888..>4561)
                     /locus_tag="OO471_004072"
     CDS             complement(3888..>4561)
                     /locus_tag="OO471_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6245113.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1063 a         1285 c         1102 g         1111 t
ORIGIN      
        1 agcccggttc gcccgggctc tgcaccgata atacacttaa ctgtatgcat gcagcgtacg
       61 ttggcaaagt gccgaacgta cgcaatcctc ttttccgaac cggacgatcc caaccatttc
      121 atcttcttcg aaacgttcca gcgcgtcact taatccggag cacacgccgc gaggcaaatc
      181 gcattgcgtg atatcaccgt tgacgataac cgtcacgttc tccccgaggc gggttaaaaa
      241 cattttcatt tgcgcggcag tcacattctg cgcctcgtca agaatgacaa ctgcattttc
      301 aaaggtacgt ccacgcatat aggcgaacgg cgcggtttcc accttcccta tttccggtcg
      361 caggcagtac tgcataaagg aagcccctaa gcgccggacc agcaagtcgt agaccgggcg
      421 aaaataggga gcaaactttt ctgcgatatc accaggtaag aagccaagat cttcatcggc
      481 ttgcagaact ggacgggtga cgataatcct gtcgacatcc ttatgtatca gggcctctgc
      541 cgcttttgct gcgctgatcc aggtttttcc gcacccggct tcgcccgtgg cgaatatcag
      601 ctgcttactc tcaatagcct gcagatagtg caattgcgct tcatttcgcg caaggatggg
      661 cgaggtatcg cgactgtcgc gggccatacc aatggcttct acgccgctca tctgcacaag
      721 cgaggtgacc gattcttctt cacgctgctt atggctgcgc gaatcccgtc tcagcacacg
      781 ttttgcctcg cgacgagctt tgatcactgc tttttgtctt cccatggaga gcaccttgag
      841 ttgtttgtat tcatcacacg cgccgttggc agcgcgatta tgcgcacgaa catcagaggg
      901 ttggcttcct tgtaagccat agtttgcttt tggataaaat gccgaaaaac ggctacgcac
      961 accgtttgcg gcgtcggtaa cacgagtaaa gaaaggaagt gagaaaaata cagagtgacg
     1021 aagttgctac cggaggagaa atctccgcga gtggtggagc gttgcttaac aaaacattgt
     1081 ccagaacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     1141 tccgctgcta cttaataagt acaacagaac tcgcattcat tgcaacaata tattcactta
     1201 tatttaacga taacacccat ttcggtgaca ttaatttcta ctggaaagat gacagagtga
     1261 tgacagtgat ggaaaaagct gtgtgctttc cgcaggattc gcagggctgg taaaatgtgc
     1321 ctcgtgtttg ccggattcgg cgcgaacgcc ttacccgacc tacaagtgat taataatttg
     1381 tgcacaaaac tcatgcccga taaacctgcg catcgggcat atttgcgtcg ttcaaacctg
     1441 caatagcgcc cttcccagat aatcgttcgc gtccttcact cccggcagcg aaaaaaaata
     1501 accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac
     1561 tgtcaggaag cctttttcca gatcgtgttg gtagcagaca aacagcaacc ccatatccag
     1621 ttgtccggag ttggtgaccc ccaacgaata actgtagccg cgacgcagca tcaggctgga
     1681 ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg cgatcacctt
     1741 cccttccggg tcgctggcgt aatcaggcac atcatgctca tgctgcattc ccagcggcgc
     1801 accggtttgc ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca
     1861 aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc
     1921 aggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct
     1981 atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt
     2041 actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacac tgagcaaatc
     2101 cggcgtgtgt ttgatgatat cgcgcagcgc atggttcacc gtgtcctggg tgttggcgca
     2161 aatctgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt tggggaaacg
     2221 cgtcatcttc tgcagctttt ttggcatctg tggcgcaagg ccaaagcgct catcaaacaa
     2281 tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat
     2341 gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgctgctc cgccctgact
     2401 cagaaaagca aaacgctgag tcaacaagcg aaacaaccgc tcaagatcgg ctttatcgct
     2461 ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc
     2521 ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa gcgtgcccgg
     2581 tgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg caccaatccc
     2641 tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt tcatctttat actgcataac
     2701 gcttaatcca gtcccagcac accgcgaagt tgcgccagat cttccgccag cgcagtaatc
     2761 ggccctttca gcgcattccg gtcggcatcg gtcaatttgt cgtaggtttc aaaaccgtct
     2821 ttagtacggt atttcgccag aatggtatcg acctttttaa agttggcatc gacttttgcc
     2881 aacagttccg ggttggcttt ttgcagttgt ggacgcagca gatcgacaat tttctgcgag
     2941 ccttcaacgt tagcctggaa atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg
     3001 ctgattttgc tggctgccac ttcctcaatc agtccggctg cgccgccgac cacttttgaa
     3061 ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga ccacatcggt ataaagctgc
     3121 tcagcgtact gatccatccc tttggtggtg ttgtcgccaa acaatgcttt ttccagacgg
     3181 tggaaaccag tgaattttgg gtcggcggct ttttgctcgt aatcatcttc acgggcgtca
     3241 atgctgccat ccagatcgga gaacagttca gcaatcggtt caatacgctc atagtgctgg
     3301 cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc ctgctttaat cgcgtcggta
     3361 aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca tgacatacgc tttatatgca
     3421 gtaattgcac caccaagact taacagcgca tcactttgcg ccgcatccgc cgttgcctca
     3481 cctttgacga tcaacttccc tttcgggtta gtcagcagac cgcaggtcat atcgtattcg
     3541 ccaggctgta aattcgccgt cattttctgg ctaaaaccag gggcgatatt ttcccgctct
     3601 tccaccacca tcacgccttt gaggatctcc cactccagcg ccttctggct atggttctga
     3661 ataatgaact gtgttttccc ggcgttaacc gtaatggtca tcggttcgca ctgcttatcc
     3721 gtcacggtca ctttgacctg cggcacatca gcggcgttag ccataaaagc agaagaaaac
     3781 agcgcagcca cgctcaactg caatgcgtta cggcggaagt taatggtcat gacgagacta
     3841 tccctttaaa gtatgttgta actaagcaag agtttgcgtc gtatttgtta cgcggagcga
     3901 gacgctgtcg cccctgcgcg tggtggcaga gcaaatgcca ccagcgccgg gatgagataa
     3961 ataaaccaga cggcgacttc gctgacgctc ggtgcttcct gatagccaaa aatcccttcc
     4021 atcagcgtgc caaacagcga gtgagttgag aggaccgcgc tcatatcgaa ggcgatttcc
     4081 tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa tggcaccagc cgccagccct
     4141 gcggcgacga agagaataaa caggctggtc catttaaaaa atgcaccaag attgaggcga
     4201 ataccgcccc agtagagcag gaagccgagc accacggcag tagcaagacc gagcattgca
     4261 cccagcggcg gccagatccc gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac
     4321 tccagccctt cccttgcaac ggcaaaaaag accatcatca ccagcgccca gccatgatga
     4381 tttccacgct gcaatgcgct atcgactgcc tgttccagtt gcactttgac gttgcgcgac
     4441 actttgcgca tccagaaaac catccaggta aggatcacca cggcgatcac cgccacgata
     4501 ccttcaaaca gttcctgctc tttttgcgga aattcgccgg tggtttcgtt aatgaagatc
     4561 c
//
LOCUS       DAJTUM010000199         4548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTUM010000199 DAJTUM010000000
VERSION     DAJTUM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..1222
                     /locus_tag="OO471_004073"
     CDS             <1..1222
                     /locus_tag="OO471_004073"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6245114.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO471_004074"
     CDS             1222..1956
                     /locus_tag="OO471_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6245115.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2424..3185
                     /locus_tag="OO471_004075"
     CDS             2424..3185
                     /locus_tag="OO471_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6245116.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3229..4308
                     /locus_tag="OO471_004076"
     CDS             3229..4308
                     /locus_tag="OO471_004076"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6245117.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4305..>4548
                     /locus_tag="OO471_004077"
     CDS             4305..>4548
                     /locus_tag="OO471_004077"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6245118.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          864 c         1120 g         1317 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg atttttttat
     2221 tgtaggcttt tgaaggctat tataactgtg cgctaaatta atgcgttgaa taaaagaaat
     2281 aacaaaatta agaaaagcca tatttacgat atgggcaaca taaatataat ctaaaaaaga
     2341 gtaaggcgtt gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg gatttatcag
     2401 ttgtcgaaat ggtttttata ataatgaaaa attttaatgt actaaccaaa gtcgtatttt
     2461 gcttgtcgtt tttatcaagc gcatttccca cctatgctgc ggatgatgtc aaagtgactc
     2521 aaaacacttc agtttttaaa ctgaagctta gtgcaacgcg attaatctat gaacaagggt
     2581 cggatggcgc agcgataact gtgacaaatc cgcaggattg gccgatgctt gtgcagagta
     2641 aagtctttga agaagataaa gtaactgcag cgccctttat tgtcactccg ccactgttca
     2701 ggcttaatcc aggccagcaa aataaaatgt gggttgtgca gactgcgcca gctttacaag
     2761 acagggaaaa attacactgg ctatgcgcca ccggcattac gcctaaaaag agtgatgttt
     2821 gggccgagaa agataaagaa aaaattgctg aaaatgtgaa cctgaacatc caggttaacg
     2881 tacatacctg cataaaactt attaccaggc catctggtat tgaaggcaag atggttaatg
     2941 cgagtgagaa gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc aatcagtcgc
     3001 cgttctacat caacctttct tctttacagg ttggcggtag caagattgag aaactagatt
     3061 atattgcgcc gttttcgcaa catgagtttg ttatgccaaa aactagcggc caggatatta
     3121 aatggtctgt gattactgat gaaggtggtg agagtcgttt gtttatatct catttgcaat
     3181 cataatgtat tacattttta ttgaatattt ttatgccatg catattccat gttttatcga
     3241 ctgtcattaa ttttgataat ggccttactt gcgggtcagt taagtgccca ggagtggtca
     3301 tttgattctt cacagttgga aggaaacgtt agtgcggaca ctgtggcaat gtttaatcag
     3361 ggagagcaac tccccggaaa ttatcgtgtg gaaatttatc ttaacggtga aaaagttgat
     3421 gttggggagt ttccctttca tcgtccggaa tcccctgaag aaaaagagct tgttccatgt
     3481 ttaaccgttg atgaccttat ccactatggc attaaaattg ataaaagcag cagtgatact
     3541 gacaataaaa agaatcagtg ttttaaatgg aactctattg aaggtttgaa agtaaattat
     3601 gattttgata gccagcgcgt acagataacc gttccacaac tgtatctgca agataaaaaa
     3661 agtagtttag caccagtgtc gttatggaat gagggtgtag ctgcattccg catggtgtat
     3721 cagacaaata ttgatatttc caaacaaaat gataatcaat ctaccacgcg taattctcgt
     3781 tatggcaggt tcactccagg gtttaatctt ggtgcctgga ggtttcgtag ttcagtgaca
     3841 tggagcaagg aacttggcca gtcagaaaga tggcaaaggg gttacatgtg gtttgagcga
     3901 ggcataaatg cgattaaaag ccgattaacg cttggagaat cttatacgtc gtctgaggtg
     3961 tttgacagca taccttttcg cggcgggatg ttagcgaccg acgatgcgat gactccgccg
     4021 gaggacagct actacacacc tgtggtgcat ggaatagcac aaagtgaggc gcaggttatt
     4081 attaaacaaa atgggcaaat tatttttacc cgttctgtgc cgccagggcc ttttgcgctg
     4141 gataatttac caacgctggc cgttggcggg gagcttgatg ttacagtgag agagagtaat
     4201 ggcgaggagc agtatttttc cgtgccattt caaacgccgg ccatcgctct gcatgaagga
     4261 tatttcaaat atagtgtgat gggggggaat ataaagaaga aggtataaaa agctcccctg
     4321 tctccgtggg cgaaatcacg gcgatgtacg ggcttcctta tggcgtcacg gtgtatggag
     4381 ggattcaaaa cgccactcat tttaatgcaa tatctacggg tattggcatt tctctggggc
     4441 tgttagggag tttatcgaca gatattacgc gttcgattgc gaatttatat tatgggaata
     4501 aatatcgtat tcgttatagt aaatccatta gtgattttgg tactcaat
//
LOCUS       DAJTUM010000200         4445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTUM010000200 DAJTUM010000000
VERSION     DAJTUM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..227)
                     /locus_tag="OO471_004078"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="OO471_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            211..469
                     /locus_tag="OO471_004079"
                     /pseudo
     CDS             211..469
                     /locus_tag="OO471_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(578..1156)
                     /locus_tag="OO471_004080"
     CDS             complement(578..1156)
                     /locus_tag="OO471_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245119.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1577..1814)
                     /locus_tag="OO471_004081"
                     /pseudo
     CDS             complement(1577..1814)
                     /locus_tag="OO471_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2001..2430)
                     /locus_tag="OO471_004082"
                     /pseudo
     CDS             complement(2001..2430)
                     /locus_tag="OO471_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2478..2843)
                     /locus_tag="OO471_004083"
     CDS             complement(2478..2843)
                     /locus_tag="OO471_004083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245120.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
     gene            complement(3596..4069)
                     /locus_tag="OO471_004084"
                     /pseudo
     CDS             complement(3596..4069)
                     /locus_tag="OO471_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(3997..4363)
                     /locus_tag="OO471_004085"
                     /pseudo
     CDS             complement(3997..4363)
                     /locus_tag="OO471_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1086 a         1188 c          917 g         1254 t
ORIGIN      
        1 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
       61 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
      121 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
      181 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
      241 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
      301 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
      361 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
      421 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
      481 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
      541 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
      601 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
      661 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
      721 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
      781 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
      841 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
      901 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
      961 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     1021 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     1081 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     1141 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     1201 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     1261 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     1321 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     1381 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     1441 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     1501 ctccctcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     1561 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     1621 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     1681 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     1741 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     1801 tatcgacagt tcatcgatag gaatttaaat ccccaaaaga ttaaaaaagc accgaagaat
     1861 gcgcgtttat tcaacacaac ctgatcatca agtaatcggt atagacactt aatattgctt
     1921 acattgattg attcaatgta tctctgatgg ttttttgctc cgttcccctc aacatggacg
     1981 caacttcccc tctgcgactc tcagccgcac caccgtattc gagccagcgg ctcatgcatc
     2041 ataacctgct ctgccagacg gtaacccctc ttcagccccg taaaccgcat ctgacctccg
     2101 cgcagctcgc acttcagcgg gtcaaccttc agacactgct gatacatccc tctccttgtg
     2161 atttgcctcg ccgtttttct cactgtctcc gttatggcgt acaccacatc ttccagtaat
     2221 cgccgcttca ccggactcag gaccccgtat caccatccgg aaccccttat ccgccacatg
     2281 ccaggagaac ctttccataa actcgtcgcc actcatcaca aggttttctt ctcgttttgt
     2341 ccggtggctg ttgtaacgca ggccaatttc atcctgaccg gcataatgtt ccaggcggct
     2401 catctgcacc ggctgcttct ccagccagtt aagccatcgg tttccctgct gaaaaatatc
     2461 ggcaaaacgg ggaagcatca gaaaggcgga ataacgccat ccggccagtg aaccgtaccg
     2521 cattcagggc aatacatgcc gtcggcgttg ataccggcag gaattgtcgc aaattcccgc
     2581 tccgtgcagc gggcaatttc cggataccct tcgtcatcaa cacgtacaaa ccagaagacc
     2641 agttttttgt ttccggcatc cacaaagaac ggaatattca ggtctgcgca acactcaacg
     2701 gcatcgtcaa aattatcaaa gcgcagaacc tcagcttctt cttcgtcaaa aaaatcatct
     2761 tcgtgaagtt ttacgacata gcggaggagt ttgcttcttt gaggtgcagg ttttcgtttg
     2821 cgtggtttag ctgaacgggc catataacta cctgaaagac aatgatattg tctgttttta
     2881 taacggtaat cacaggccat gacaagccgc agccgtcagg ccgcctactc ggggcggttt
     2941 agaacgctta tttagtacca gatcttactt ataataaagg ggcttggcag tttggataaa
     3001 ctattgtcct tattccacta atacaaactt tttgtaaagt atattaaata tacttcattt
     3061 catactgaaa tccccggaag tattcaatca aacaattaca ccataaaagg aagagggggc
     3121 cccaattaag ttgtcaagca tgtttactgc gcaatcgctt ggtgctccgt ccctgtaccc
     3181 cgcattggta ctctggcgct catggggctt ccacttgcgc ttctcccttc acatcggggt
     3241 gacaggtcca cgttccccac aagagcctga atcgacttca cgccgctttt atgccggatg
     3301 ccgcgcaggc agtaaacata tcccacagga acgactccac cattgttttg acatcatcta
     3361 tatgctttcg acacatcatc agcggttcac tttcgttcgt ctcttcgaac catacctgat
     3421 cggctcacgt ccgacctttt cccatctcgc tcaccaccac atctcttaaa tgcagcagca
     3481 gtggatggtt tggtgcctgc gcctgcacgc cgacaccaag gggccttccc tcatctcttg
     3541 tgctgcatgg ctgcacaggg actcacgtcc ccaggacgcg ccttcgtggc acacagtcat
     3601 cggcatagca acagtaagca acggcaggtt tccattgcca gttttctttg acagcagtac
     3661 ttcggcctcg ctggatactg tgattccagt accagcgatc ctttctggct tttccactca
     3721 ggtagcgttt attcagatac tggtcgaact cattcagtat gatgttcgac agcaacggcg
     3781 agatcacacc gccctgtggc atactttcac tgaccgcccg gaagagacca acatcaacac
     3841 ggcgttttcc acagcagtct cataaaacgt gtatcactga tcctgcggca gacggctttc
     3901 atcagcagcc ggtgatgtac tgtgtcgaag tagctggaca ggtcaccttc aataacccgg
     3961 cgccccgtgt ttccccgcag tcggtgagct gtaatagtac gtgatgtctc ccgcccactt
     4021 ctggttcggg ccactggcgt aaaaatcctg cgtcagcaga ttttctgata caggcaggcc
     4081 gtgtgcacgg taactgatcg ggctgaactt ctgggaggct tttgcgctca gttcctgacg
     4141 gcgcagactg gctgccacgg tttttacgtt aaagtggtaa ccagagcaca cagttcatcc
     4201 gtcaggcgtg gggcaccgta acgctggact gggtaaaagc cttgcggaca acgctgtcgc
     4261 agtgctgacg gaactgcgga cgtgtgttta tccttgtccg ccgctgacac cacgtatacc
     4321 agccgctgcg ggccacccgg agtacgcggc acattgcctt gatgacggtt tacgcggtcg
     4381 cctgcggcaa cggctttccg gctgacggta gtgtggacct ttttaccccg gagaacaccg
     4441 gcaag
//
LOCUS       DAJTUM010000201         4212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTUM010000201 DAJTUM010000000
VERSION     DAJTUM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..438
                     /gene="tnpA"
                     /locus_tag="OO471_004086"
                     /pseudo
     CDS             1..438
                     /gene="tnpA"
                     /locus_tag="OO471_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(556..1500)
                     /gene="hypT"
                     /locus_tag="OO471_004087"
                     /gene_synonym="qseD"
     CDS             complement(556..1500)
                     /gene="hypT"
                     /locus_tag="OO471_004087"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6245121.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1845..2564
                     /locus_tag="OO471_004088"
     CDS             1845..2564
                     /locus_tag="OO471_004088"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6245122.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2630..3928
                     /locus_tag="OO471_004089"
     CDS             2630..3928
                     /locus_tag="OO471_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR6245123.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          914 c         1036 g         1202 t
ORIGIN      
        1 cgcacacccg atggaactgt aaatatcaca tagttttttg cgccaaaata ccgaagacag
       61 gtgttctaca gagagaagcg tagagcaaca ggcagtattt tgagaaagct gtgtgagtgg
      121 aaaagcgtac ggattctgga agctgaatgc tgtgcagatc atatccatat gcttgtggag
      181 atcccgccca aaatgagcgt atccggcttt atgggatatc tgaaagggaa aagcagtctg
      241 atgctttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga gttctggtgc
      301 aggggggact acgtcgatac agtgggtaag aacacggcga agatacagga ttacataaag
      361 caccagcttg aagaggataa aatgggagag cagttatcga ttccctgtcc gggcagcccg
      421 tttacggacc gtaagtaacg aagttggatg caaatgtcag atcgtgtgcg cctgttaggg
      481 cgcggctggt aagagagcct tataggcgca tttgaaaaac ctccggctat gccggaggat
      541 atttattgca cgtcgtcaat tgcggcgtgc tggctcttcc attgatatct gctggattaa
      601 atcgtctggc atatgttcct gtaaaatacg ccagaggttc tcggcggttc tattcagtcg
      661 ggtgttcatt cggtaaatat aagcctgaat ggggactgtc aatttagcag catcaaggca
      721 tatcagacgt ttggtctgta gttcatcgac gatcgaccag atcggtaacc aggcaatgcc
      781 gtatccgttg agcgccatat tctttaataa ctcgctcatc gaagagataa atatcgtgcg
      841 cccggtaata cctccaacct ctgccagatg acgattgacc aacctgccca tataagatgt
      901 ggcggtatag ttgagcaaag gaacctgggg ctgagtgata tcgaatacag ggtgcccctg
      961 tgcgtcggcg gcacataccg ggtacaactc agattcaaac agtttcaggc agcaaaaagg
     1021 cgattgcata agatcttcat cgcgaaacga aataataaag tcactcttgc cttcgcgtaa
     1081 cgtatttacg gcctggacaa catcaatggc ttcaacatga tagacaaatt ctccgccata
     1141 ggcggtaagt gaatgcacca gtttgggtaa tacgctcagg gatagtgagt gcgcggctgc
     1201 aattttaatg ttgggaacgc cgagtaaact ctggccgttt aactccccca aattgcattc
     1261 aagctgctgt aataagctgc gagtctggga atgaaagagc ttgccctctt cggttaattg
     1321 taaaggggtg gtcgtgcgat cgaataaaac aacgccgatc gctgcctcca gcgccttaat
     1381 tcgtcggctg aatgcgggtt gcgaaagatt acgctctttt gccgcctgag aaaaatggcg
     1441 acaggcttcc agtgtcagga aatcgtataa ccattttgtc tcgatattct tcattaccat
     1501 ggcacctgta ttttcaatcc aaagacataa taaaacatcc cggcaataaa aataatgttc
     1561 tcaattagca caacctggcc tttatcattc gttttgtgca taaccccttc atttccggta
     1621 ttgacgttga tatcacattt cgccaaaaaa acgaaatttt tccgtcagat aaccagaata
     1681 catcatcact atggtgaata agcggaatgt tccatagctg catcatgcgt gtcttgcatc
     1741 gccttgtaca accttgcatt tctcatcgcc catttcgact gttaaaaaag agagcagcag
     1801 ttatttaccg cgtattgtca ttaacgtttt gagaggcagg tattgtgaaa ggtttaattg
     1861 gtgtactcgg tggaatgggg ccggcagcta ccgtcgacct gtttaataaa tttgtaactt
     1921 ttaccgctgc acaatgtgat caggaacata ttcctttaat tatttcttcg atcccggata
     1981 ttcctgaccg taccgatgca ttaatgcatc atgggcgttc accattaccc gttatgcgtg
     2041 attatatgca taagctggaa gatgccgggg cagaatgtat tgttattccc tgtaataccg
     2101 cgcattactg gttcaaagaa ttaaaagatg cttgtcatat tgatatatta agtatcgtag
     2161 aaactacgat caatgaagtt cgcgcctgtg gtaaaacccg aattggcttg ttagccacca
     2221 atgccacgct atatatgggt ttataccaaa aaggaattga gtcgctggga tttacctgtg
     2281 tcagccccga tgccgacggt cagaaaaaag tgatggaaag tatttattcc ttgaaagctg
     2341 gcaatatagc gcacgcacaa aagttgatga atgagcaggc tgaaatatta ttttcccgcg
     2401 gtgcagaaat ccttgttctt ggttgtacag aaattccggt tattctggcg caagccgtta
     2461 aagagcagcc ttcccgctat attgactcaa cggcgtcact cgttcgtgcc ggaataaaat
     2521 ggtacgaaaa tcgtgtcggt aaacattatc ttttaaccca ataatcattt aaatcgcagc
     2581 cagaaagtta ttcgctttta actgaattat atttataacg gagaacatta tggtttggct
     2641 ggaaattatc gtagtacttg gtgcaatatt ttttggtatt cgccaggggg gaatcggtat
     2701 tggtttatgt ggcgggcttg ggcttgccat tctgactctg ggatttggtc tgcctatggg
     2761 gtcgccgcca gtcgacgtga tcctgattat tatgaccgtt gttgtggcgg cctcagcctt
     2821 acaggccgca ggcggaatgg attatctggt acgcgtcgct ggtaatttta tgcggcgtaa
     2881 cccgaaatat atcaacataa tcgcgccgat tatcacctgg tctatgacca ttatggccgg
     2941 tactgggttt attgtttttt ccacgctgcc ggtgattgct gaagtggcga aagagtccgg
     3001 tatccgtcct tcccgcacgc ttgccggttc ggttgtcgct tcgcaggttg cgatttccgg
     3061 ttcgccgatc agcgccgcaa tggcggctat gctcactatt atggaggtaa acggtgtcag
     3121 ttttattcaa gtgatgtcgg tatgtcttcc cacctctttt gctgccgcga tggtcgccgc
     3181 ttttattgct tcccgacagg gatgtgagtt gcaggatgac gaggtctacc ttgagcgcct
     3241 gcaaaaagga ctggtgcaaa aatacgagaa taatagcagc atcaagccag gtgcgacgct
     3301 ctccgtcgga ttgttcctgc tggcgacggt cgctattgtt attcttgctg ccttccccca
     3361 actgcgtccg ggttttgata tcggtaaacc gatggaaaca cgcgatatca ttattatctg
     3421 tatgctctct gccgcctgcc tgatggtggt gctgtgtaag acggcgaccg atgccattat
     3481 tctcacctcc acattccgtg cggggatgag ttcgctggcg gtaattctcg gcattgtaac
     3541 cttaggcacc acttttattg atgcgcattt aactgagatt aaagatattg ctggtgatat
     3601 tttgcaggcg tatccgatgt tgttggcgct ggttcttttt ggcacttgcg cgctgctcta
     3661 ctcgcaaggt gcgaccacgc cgttaattat cccgcttgcc gtcgcgcttg acgtgcccac
     3721 ctgggcaatt ctggcgtcct atgtggccgt caccggagta tttgtactgc caacgtatcc
     3781 aacatcactg gctgccatgg aatttgatac taccggcaca actcgtgtag gcaaatatgt
     3841 gctgaatcat ccatttatgc tgcctgggtt ggggggaatt attgctggag tggcattagg
     3901 ctttgtgatt gcgccaatga ttatttagca ctatcaaaca tcagtatcga tggtttatac
     3961 acaataaaga cagatatggc ggaatatagt ctgttgtaag cgccaacaaa gaatagggga
     4021 attccgccat gtctgaacat cttcacaata tggtattaag tgttgtcgtt taatgattta
     4081 tttcttggtt atgttgtgta ttagctgcat ctatcgcttt ttgatacctg aggtgtactg
     4141 gcaatagcgg acactaccat ttgttctttt tttaagcagt catctgatga tatttttccc
     4201 tgaaggctgc cg
//
LOCUS       DAJTUM010000202         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTUM010000202 DAJTUM010000000
VERSION     DAJTUM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO471_004090"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO471_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6245124.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO471_004091"
     tRNA            complement(324..399)
                     /locus_tag="OO471_004091"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO471_004092"
     tRNA            complement(406..480)
                     /locus_tag="OO471_004092"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO471_004093"
     tRNA            complement(597..681)
                     /locus_tag="OO471_004093"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO471_004094"
     tRNA            complement(690..765)
                     /locus_tag="OO471_004094"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO471_004095"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO471_004095"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6245125.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO471_004096"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO471_004096"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6245126.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO471_004097"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO471_004097"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6245127.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTUM010000203         4198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTUM010000203 DAJTUM010000000
VERSION     DAJTUM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            84..752
                     /locus_tag="OO471_004098"
     CDS             84..752
                     /locus_tag="OO471_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245128.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO471_004099"
     CDS             complement(995..1690)
                     /locus_tag="OO471_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6245129.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO471_004100"
     CDS             complement(1683..3110)
                     /locus_tag="OO471_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6245130.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO471_004101"
     CDS             complement(3121..3840)
                     /locus_tag="OO471_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6245131.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAJTUM010000204         4188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTUM010000204 DAJTUM010000000
VERSION     DAJTUM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="OO471_004102"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="OO471_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6245132.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="OO471_004103"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="OO471_004103"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6245133.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1169 a         1021 c          961 g         1037 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcattggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccacgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttctgg
//
LOCUS       DAJTUM010000205         4166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTUM010000205 DAJTUM010000000
VERSION     DAJTUM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO471_004104"
     CDS             complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO471_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6245134.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO471_004105"
                     /pseudo
     CDS             complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO471_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="OO471_004106"
                     /pseudo
     CDS             complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="OO471_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3785..>4166
                     /locus_tag="OO471_004107"
     CDS             3785..>4166
                     /locus_tag="OO471_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR6245135.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1207 c         1021 g          901 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcgctta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacatagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     3481 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     3541 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     3601 cgttcaatgg tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata
     3661 ggttcagttg cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc
     3721 tatagaatcg cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga
     3781 tcaaatggaa tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac
     3841 caacatagca tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga
     3901 ccgattactt tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt
     3961 agatcaaaat gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc
     4021 tcgcgggata ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc
     4081 cacggggatt gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca
     4141 cgatctcggt aatccaccat ttgggc
//
LOCUS       DAJTUM010000206         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTUM010000206 DAJTUM010000000
VERSION     DAJTUM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..158)
                     /locus_tag="OO471_004108"
     CDS             complement(<1..158)
                     /locus_tag="OO471_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6245136.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO471_004109"
     CDS             complement(308..469)
                     /locus_tag="OO471_004109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245137.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO471_004110"
     CDS             complement(466..654)
                     /locus_tag="OO471_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6245138.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO471_004111"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO471_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6245139.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO471_004112"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO471_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO471_004113"
     tRNA            complement(2161..2237)
                     /locus_tag="OO471_004113"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO471_004114"
     tRNA            complement(2339..2414)
                     /locus_tag="OO471_004114"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO471_004115"
     CDS             complement(2559..3380)
                     /locus_tag="OO471_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6245140.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO471_004116"
     CDS             complement(3395..3751)
                     /locus_tag="OO471_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6245141.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="OO471_004117"
     CDS             complement(3764..>4114)
                     /locus_tag="OO471_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6245142.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTUM010000207         4043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTUM010000207 DAJTUM010000000
VERSION     DAJTUM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(886..1077)
                     /locus_tag="OO471_004118"
     CDS             complement(886..1077)
                     /locus_tag="OO471_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6245143.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="OO471_004119"
     CDS             1142..1465
                     /locus_tag="OO471_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6245144.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO471_004120"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO471_004120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO471_004121"
     CDS             2143..2673
                     /locus_tag="OO471_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6245145.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTHWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO471_004122"
     CDS             2680..2913
                     /locus_tag="OO471_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6245146.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3460..3852)
                     /locus_tag="OO471_004123"
     CDS             complement(3460..3852)
                     /locus_tag="OO471_004123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245147.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          918 c         1208 g          951 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 cactggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggg caccgggaac gcgcttcagt cagaccttgc
     3121 tgaaaacaca ggaacgagtt aaaacgcata cgggcgcgat tctggtgtgt cttttgtcgc
     3181 tgtgttttgc ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctca
     3241 ggtccccgtc ttcttcgctt ccgaaagcat gaataaaatc cgtttcagct gtctctggct
     3301 ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg
     3361 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     3421 cgcgccgaac gcgtttcagc ggtgcgtaca attaagatat tacgttaaat tgtgagggaa
     3481 tggtccccat atactgactt tatcataggg atagggttgg tagtgggtcg attgggtttc
     3541 ggcattgttt ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga
     3601 ttctcctggg cctggaaagg ctaagtgttt agaattgatg gcaaaatatc ttccgtccga
     3661 tttttgaagt agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat
     3721 ttgtagtttt tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg
     3781 tattattctc tcttttaata agctgtttac cacagggttt agttgttgtc taaagcacgc
     3841 agataatgcc attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg
     3901 tgtttgatgt gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac
     3961 ggctataaaa caaaattttc atagagtgat tcaagatctt cttgagatcc ttttttgcgg
     4021 cgcgtaatct gctgcctgca aac
//
LOCUS       DAJTUM010000208         4017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTUM010000208 DAJTUM010000000
VERSION     DAJTUM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(162..2195)
                     /gene="metG"
                     /locus_tag="OO471_004124"
     CDS             complement(162..2195)
                     /gene="metG"
                     /locus_tag="OO471_004124"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6245148.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2327..3436
                     /gene="apbC"
                     /locus_tag="OO471_004125"
     CDS             2327..3436
                     /gene="apbC"
                     /locus_tag="OO471_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6245149.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3698..3979
                     /locus_tag="OO471_004126"
     CDS             3698..3979
                     /locus_tag="OO471_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6245150.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          988 a          991 c         1038 g         1000 t
ORIGIN      
        1 ctgatagata aagtgtctca ttctgcatcc ctataatttt atccagttga atgcagatgc
       61 taccagtatt tatacgggtt agagagagac aaaatcgcaa gaaaacaact ttaggaattt
      121 ataaagaaaa ggcgctgtcg atgcagcgcc ttgaaggggg attatttcac ctgatgaccc
      181 ggtttagcac cggcatccgg gcttagcagg aaaatatctt tcccgccagg accggcagcc
      241 atcaccatgc cttcagagat accgaagcgc attttacgtg gtgccaggtt agccaccata
      301 atggtgtgac gaccaatcag tgcctgcgga tccgggtaag cagaacgaat accggagaag
      361 acattgcgtt tttcaccgcc gagatccagc gtcaggcgca gcagtttgtc agaaccttca
      421 acaaactctg cgttttcgat cagcgccacg cgcaggtcaa ctttggcgaa gtcgtcaaag
      481 gtgatggttt cctgaatcgg atcatctgcc agtgggccag taactggcgc ggtagtggct
      541 ttcacttctt ctttagaggc ttccaccaga gcttcaacct gtttcatatc gatgcggtta
      601 tacagcgcct tgaacggatt cactttgtgg cccagcagcg gttgctggat accatcccag
      661 gtcagttccg tattgaggaa ggcttctgca cgctcggtca gtttcggcag tacaggcttc
      721 aggtaagtca tcagcacgcg gaacaggttg atgcccattg agcaaatcgc ctgcaggtca
      781 gcatcgcgac cttcctgttt tgcgactacc cacggagcct gctcgtcgac atagcggtta
      841 gccaggtcag ccagtgccat gatttcgcgc actgctttac caaattcacg gctttcccac
      901 gcttcaccaa tcacttcagc ggcatcggta aaggttttgt acagctcagg gtcagccagc
      961 tcgcttgcca gcacaccgtc aaaacgcttg ttgataaagc ccgcattacg ggaggccagg
     1021 ttaaccactt tgttcacgat atcggcattc acacgctgaa cgaaatcttc caggttgaga
     1081 tcgatatcat caatgcgcga agagagtttc gcagtgtagt agtaacgcag gctgtcagcg
     1141 tcaaaatgat tcagccaggt gctggcttta ataaaggtgc cgcgagactt ggacatcttc
     1201 gcgccgttca ccgtcacata gccatgaaca aacaggttgg tcggcttgcg gaagttgctg
     1261 ccttccagca tggcaggcca gaacaggctg tggaagtaaa cgatatcttt accgatgaag
     1321 tggtacagct cggcggtgga gtctttcttc cagtattcat cgaagcttac gctgtcgccg
     1381 cgcttgtcgc acagattctt gaaagaaccc atgtagccaa tcggtgcgtc cagccagacg
     1441 tagaaatatt tgcccggtgc gttcggaatt tcaaaaccaa agtatggcgc gtcgcgggag
     1501 atatcccact gttgcaggcc ggattcaaac cactcctgca ttttgtttgc cacctgctcc
     1561 tgcaacgcac cgctgcgggt ccatgcctgc agcatttcgc tgaaagaggg cagatcaaag
     1621 aagaagtgtt cagaatcacg catcactggt gtagcaccag aaaccaccga tttcggctcg
     1681 atcaattcag tcgggctgta ggtcgcgccg cagacttcgc agttatcgcc gtattgatcc
     1741 ggggatttac atttcgggca ggtgcctttc acaaaacggt ccggcaggaa catgcctttt
     1801 tccggatcgt acagctgaga gatggtgcgg tttttaataa aaccgttttc tttcaggcga
     1861 gagtagataa gttctgacaa ctggcggttc tcttcgctgt gcgtcgagtg atagttgtca
     1921 tagctgatgt taaagcctgc gaaatcagtc tgatgctcct gactcatttc gccaatcatc
     1981 tgctccgggg tgataccgag ctgctgagct ttcagcatga tcggtgtacc gtgggcatcg
     2041 tcggcgcaga tgaagttgac ctcgtggccg cgcattcgct ggtaacggac ccagacatca
     2101 gcctggatgt gctccagcat atggccgagg tggattgagc cgttagcgta cggcagtgcg
     2161 cacgtcacca gaattttctt cgcgacttga gtcatagtag gcattacttc ttaatagtga
     2221 aaaggggtta gatagtacca aatgggaaaa tgttaagtaa gtacttctct atattcttca
     2281 ggtaaactat cagctgatgg tttatctata caaaaaagga gtcgggatga acgaacaatc
     2341 ccaggccaaa tcaccagaag ccttgcgcgc aatggtcgcc gggacactgg ccaattttca
     2401 gcacccaacc ctgaagcata acctcaccac gctgaaagcg ttgcaccatg tcgcctggat
     2461 ggacgacacg ttgcatgtgg aactggttat gccgtttgtc tggcatagcg cgttcgaaga
     2521 gttaaaagag caatgtagcg ccgaactgct gcgcatcacc ggggcgaaag ctatcgactg
     2581 gaaactgtcg cataacatag ccactctgaa acgcgtgaag aatcagccgg gaattaacgg
     2641 tgtgaagaac attatcgcgg tgagttccgg taaaggcggc gtgggtaaat cgtccacggc
     2701 ggtaaacctg gcgctggcac tggcggcgga aggggcgaaa gtcggcattc tggacgccga
     2761 tatctacggc ccatcaattc ccaccatgct gggcgcagaa aatcagcgtc caacttcgcc
     2821 ggacggtacc cacatggcac cgattatgtc tcatggcctg gcaaccaatt caattggtta
     2881 tctggtcact gacgacaatg cgatggtgtg gcgtggaccg atggcgggca aggcgttaat
     2941 gcagatgttg caggaaacct tgtggccgga tctcgactat ctggtgcttg atatgccgcc
     3001 aggcaccggt gacattcaat tgacgctggc gcaaaacatt ccagtaactg gtgcagtcgt
     3061 ggtcaccacc ccgcaggaca tcgcgttgat cgatgcgaaa aaaggcattg tgatgttcga
     3121 gaaagtcgag gttccggtgc tcggtattgt cgagaacatg agtgtgcata tttgcagtaa
     3181 ctgtggtcac catgaaccga ttttcggtac cggtggtgca gagaaactgg ctgaaaaata
     3241 ccatacacag ctgctggggc agatgccgtt acacatctcc ttacgtgagg atctcgataa
     3301 aggcacgcca acggttatca gccgtccgga gagcgaattt accgctatct accgccagct
     3361 ggctgaccgc gttgcagctc agctctactg gcagggtgaa gtcattcctg gcgagatttc
     3421 cttccgcgcg gtctaacgcc tattaaccat tctggttatt cttcatacac cgcagagatg
     3481 ttatattgat gcggtgtatt tgtttttgcc gcattaatga acacttattt aatactgcga
     3541 atattctgcg gaccttcgtc cgaaactcta caaagtcgag tgatcgtaca ggcaatgctt
     3601 cattatcatc cttcgtgctg tgtagtggat gtacagctat agtttgttat gcactcattc
     3661 tatccaggca taacctttac tacagggata acgttcaatg aataagtatt ggctgtcagg
     3721 aattattttt cttgcctatg gattggcttc tcccgcagtc tcttcagaga ctgccacatt
     3781 aacgattaat ggcaggatca gtcccccaac atgcagtatg gctatggtta atagtcaacc
     3841 acaacagcat tgtggtcagt taacgtataa tgttgacacc cgtcatcagg tttcttcgcc
     3901 cgtcaaaggt gttacgacag aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt
     3961 gttaaatcgc tacgattaat ttggcaacct gattttaatt gcatttagaa agacgaa
//
LOCUS       DAJTUM010000209         3810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTUM010000209 DAJTUM010000000
VERSION     DAJTUM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(226..429)
                     /locus_tag="OO471_004127"
     CDS             complement(226..429)
                     /locus_tag="OO471_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245151.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OO471_004128"
     CDS             complement(431..586)
                     /locus_tag="OO471_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6245152.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OO471_004129"
     CDS             complement(789..1196)
                     /locus_tag="OO471_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6245153.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OO471_004130"
     CDS             1273..1500
                     /locus_tag="OO471_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6245154.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OO471_004131"
     CDS             1484..1906
                     /locus_tag="OO471_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245155.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="OO471_004132"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="OO471_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3514
                     /locus_tag="OO471_004133"
                     /pseudo
     CDS             2768..3514
                     /locus_tag="OO471_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3529..>3810
                     /locus_tag="OO471_004134"
     CDS             3529..>3810
                     /locus_tag="OO471_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6245156.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          974 a          836 c         1045 g          955 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggttaat tttaactggg agagctggcg
     3481 tccgaatgtt agccattcga gggttgttaa gtagtttcag gaggatttat ggcgaaacct
     3541 tttactcccg aacagcggga agaactgaag acgcgaattg tggaactcgt gcatcaggac
     3601 ggtcgggtca cgattcggca gttgtccgat gaaacaggta tcagtcgtgc gtctgtcggt
     3661 cgcttatgca tagaactggt cgcaagtggt gatgtatata attctggcta cggcttattc
     3721 ccgtctgaac aggctcgcaa ggactggcaa agcgcccgca aaaaactctc gagagtaaag
     3781 gtgaggaaac cggttgttgt tgatccggac
//
LOCUS       DAJTUM010000210         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTUM010000210 DAJTUM010000000
VERSION     DAJTUM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            437..2089
                     /locus_tag="OO471_004135"
     CDS             437..2089
                     /locus_tag="OO471_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6245157.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO471_004136"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO471_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO471_004137"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO471_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO471_004138"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO471_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTUM010000211         3725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTUM010000211 DAJTUM010000000
VERSION     DAJTUM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..723
                     /locus_tag="OO471_004139"
     CDS             <1..723
                     /locus_tag="OO471_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6245158.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVART
                     VLRAVRDTVVSVARAVWNWIFF"
     gene            1095..>3725
                     /gene="ag43"
                     /locus_tag="OO471_004140"
     CDS             1095..>3725
                     /gene="ag43"
                     /locus_tag="OO471_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6245159.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
BASE COUNT          854 a          964 c         1121 g          786 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
      241 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
      361 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
      541 gacgagctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg tacggttctg
      661 cgtgccgtcc gtgacacggt ggtctctgtt gcccgcgctg tatggaactg gattttcttc
      721 tgaacagtct gccagggaaa tgcctgataa cattacgtta accaactgaa aacatgacac
      781 aaacccctca tactcctcct gtttctcttg cgtgaccgcc ttgtcgttaa tagaataaaa
      841 ctatcgataa aacagatatc gatcgttgct atcgatcgtt tatagtggtt aatggtctgc
      901 gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaacg gactgatact
      961 tctctggcgg atgagagagg ggaagcattt atgtccggga aaaaccactt tatctgacca
     1021 tgctgttttg tacctgccgg tatccacttt tgtgggtacc ggctttttta ttcaccctct
     1081 gtaaggaaaa gctgatgaaa cgacatctga acaccagcta caggctggta tggaatcaca
     1141 ttacgggcac cctggtggtg gcttccgaac tggcccgctc acggggaaaa cgcgccggtg
     1201 tggcggttgt gctgtctctt gctgctgtca catcagtccc ggcactggct gctgacacga
     1261 ttgtacaggc gggagaaacc gtgaacggcg gaacactgac aaatcatgac aaccagattg
     1321 ttctcggtac ggccaacgga atgaccatca gtagcggtct ggagtatggg ccggataacg
     1381 aggccaatac cggcgggcaa tggatacaaa atggcggtat cgccaacaac actactgtca
     1441 ccggtggtgg tcttcagaga gtgaatgccg gaggaagcgt ttcagacacg gttatcagtg
     1501 ccggaggcgg acagagcctt caggggcagg cagtgaatac cactctgaac ggcggtgagc
     1561 agtgggtaca tgaaggcggg attgcaacgg gtaccgtcat taatgagaag ggctggcaga
     1621 ccgtcaaatc cggcgcaatg gcaaccgaca cggttgtgaa taccggcgcg gaagggggac
     1681 cagatgcaga aaatggtgat accgggcagt ttgttcgcgg aaatgccgta cgtaccacta
     1741 tcaatgaaaa tggtcgtcag attgtggctg ctgaaggaac agcaaatacc actgtggttt
     1801 atgccggcgg cgaccagacg gtacacgggc atgcgctgga taccacactg aatggcggtt
     1861 accagtatgt gcacaacggc ggtacggcgt ctggcactgt tgtaaacagt gacggctggc
     1921 agattatcaa ggaaggtggt ctggcggatt tcaccaccgt taaccagaaa ggcaaactgc
     1981 aggtgaacgc cggtggtacc gccacacatg tcaccctgaa acagggtgga gcactggtta
     2041 ccagtactgc ggcaacggtt cttggcagca accgtctggg caatttcacg gtggaaaacg
     2101 gtaaggctga cggagttgtt ctggagtccg gcggtcgcct ggatgtactg gaaggccatt
     2161 cagcgcagaa aacacgggtg gatgacggcg gaaccctggc agtgtctgcc ggtggtaagg
     2221 caacaggtgt caccatgaca tccggtggtg ccctgattgc agacagtggt gccactgttg
     2281 aggggaccaa tgccagcggt aagttcagta ttgatggcac atccggtcag gccagcggac
     2341 tgctgctgga aaatggcggc agctttacgg ttaatgccgg aggactggcc agcaacacca
     2401 ctgtcggaca tcgtggaaca ctgacgctgg ctgccggggg aagtctgagt ggcagaacac
     2461 agctcagtaa aggtgccagc atggtactga atggcgatgt ggtcagtacc ggcgatattg
     2521 ttaacgcagg ggagattcgc tttgataatc agacgacacc ggatgccgcg ctgagccgtg
     2581 ctgttgcaaa aggcgactcc ccggtaacgt tccataaact gaccaccagt aacctcaccg
     2641 gtcagggtgg caccatcaat atgcgtgttc gccttgatgg cagcaatacc tctgaccagc
     2701 tggtgattaa tggtggtcag gcaaccggca aaacctggct tgcgtttaca aatgtcggaa
     2761 acagtaacct cggggtggca acctccggac agggtatccg ggttgtggat gcacagaatg
     2821 gcgccaccac agaagaaggt gcgtttgccc tgagtcgccc gcttcaggcc ggcgccttta
     2881 actacaccct gaaccgtgac agcgatgaag actggtacct gcgcagtgaa aatgcttatc
     2941 gtgctgaagt ccccctgtat acatccatgc tgacacaggc aatggactat gaccggattc
     3001 tggcaggctc ccgcagccat cagaccggtg taaacggtga aaataacagc gtccgtctca
     3061 gcattcaggg cggtcatctc ggtcacgata acaacggcgg tattgcccgt ggagccacgc
     3121 cggaaagcag cggcagctat ggcttcgtcc gtctggaggg tgacctgctg agaacagagg
     3181 ttgccggtat gtctctgacg acaggggtgt atggtgccgc aggccattct tccgttgatg
     3241 ttaaggatga tgacggttcc cgcgccggca cggtccggga tgatgccggc agcctgggcg
     3301 gatacctgaa tctgacacac acgtcctccg gcctgtgggc tgacattgtg gcacagggaa
     3361 cccgccacag catgaaagcg tcatcggaca ataacgactt ccgtgcccgg ggctggggct
     3421 ggctgggctc actggaaacc ggtctgccct tcagtatcac tgacaacctg atgctggagc
     3481 cacaactgca ctatacctgg cagggactta ccctggatga cggccaggat aacgccggtt
     3541 atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc
     3601 acaacgatat gagctttggt gaaggcacct catcccgtga cactctgcgc gacagtacaa
     3661 aacaccgtgt gagtgaactg ccggtgaact ggtgggtaca gccttctgtt atccgcactt
     3721 tcagc
//
LOCUS       DAJTUM010000212         3687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTUM010000212 DAJTUM010000000
VERSION     DAJTUM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..221
                     /locus_tag="OO471_004141"
     CDS             <1..221
                     /locus_tag="OO471_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6245160.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="OO471_004142"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="OO471_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6245161.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="OO471_004143"
     CDS             515..1105
                     /locus_tag="OO471_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6245162.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="OO471_004144"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="OO471_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6245163.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="OO471_004145"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="OO471_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6245164.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="OO471_004146"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="OO471_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6245165.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="OO471_004147"
     CDS             3454..3645
                     /locus_tag="OO471_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245166.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          997 a          901 c          928 g          861 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagttac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga caaatatgca
     3121 cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc cctcataact
     3241 gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttggca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttga
//
LOCUS       DAJTUM010000213         3413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTUM010000213 DAJTUM010000000
VERSION     DAJTUM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..785)
                     /locus_tag="OO471_004148"
     CDS             complement(<1..785)
                     /locus_tag="OO471_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6245167.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
     gene            complement(940..1098)
                     /locus_tag="OO471_004149"
     CDS             complement(940..1098)
                     /locus_tag="OO471_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6245168.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(1169..1387)
                     /locus_tag="OO471_004150"
                     /pseudo
     CDS             complement(1169..1387)
                     /locus_tag="OO471_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            1582..3123
                     /gene="istA"
                     /locus_tag="OO471_004151"
     CDS             1582..3123
                     /gene="istA"
                     /locus_tag="OO471_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCR6245169.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            3138..>3413
                     /gene="istB"
                     /locus_tag="OO471_004152"
     CDS             3138..>3413
                     /gene="istB"
                     /locus_tag="OO471_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR6245170.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQG"
BASE COUNT          873 a          858 c          858 g          824 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
      481 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
      541 tgttttgtgt atccccggcg gcccgggtcg cgcacgcggg tctggcaggc gaagaatggc
      601 tgaaaacctt cctgctgcag gctttccagt acggtgatgg tgggaatgta cgcatatcgt
      661 ttactgcggg aagtatgccg gtcttcaccg aaaatactgg gaacatagtg catcagttct
      721 tcgtgtgtca gcggacggtc acggcgtatc tggttcgcat aaccaaaacg actggctagt
      781 cgcataattt gctccttatc ggtggttacg atttactggt gtaataaatg aaaaagctgc
      841 gtctcccgga gaagacgcgg cctgacagat gaaatgaatg acgtttattg tctgagaagc
      901 ccttaactgg cgagctgagt attaagctgt gttccggcat caccagcgca actgaccttc
      961 agcattacgg ataaccagcc gggaatatgt tccctggtca tcttcagtaa acacattgcg
     1021 gtaagctgtt ttgacggcaa cagcctgttc gcgtgagaaa gggccttcgg gcaataaacg
     1081 tgatgtacaa cccggcatat ctgttgctcc ctgaaagtaa aaagccccgg tcatgatgac
     1141 cggggcctga aggagagtga cctgattatc aaaaagtcac attcagcgtg gcctgaccgt
     1201 tataaccttc agcgctgctg ccgctgacgc tgtgggcata accggcctga acgcccaggg
     1261 tgatattttc ccggacacgg gcttccagtc cggcctgcag gtccagtgac gtgccattcc
     1321 gggacggtga gaacgtcatg ttactgccgg cggcggctgt ccccatgctc atgtcacccc
     1381 gggagctgtg cgtattttca tgaaaggaga tcactcaata acttccatcg agatcgggta
     1441 ataacatttg aacagatcgc tgaataacat cgatggagat cacttttgac tcattttgtt
     1501 attcagtgat ctccatcaat gttattggaa cttcacaggt gtgttgatct gtatcttttg
     1561 ccattccggt aaaggatacc tatgccaaca gttccaattt ctatgagaaa acttaaagaa
     1621 attcttaggc ttaaatacgg tgttggactc agccatcgac aaattggtcg tagtcttgca
     1681 atctcccctt ccgttgtatc cagatatgct aatcgggcgg ctcaacttgg cataaagcag
     1741 tggcccttac ctacaggatg ggatgataca aaactaaaac atgcgttcct tcagacccag
     1801 gttaagatga agaagcactc tctgcctgac tgggctacag tacaccggga actgcgtaat
     1861 aaatgcgtga cgctgcagct actctgggaa gaatactgtg agcgtaatcc aggcggtttt
     1921 tacagctata accattactg ccggatgtac cgtgaatggc tcaaaaccac ttcaccatca
     1981 atgcgtcagg tacataaagc tggcgaaaaa cttttcgttg attactgtgg acctaccgtt
     2041 ggcgttaccg accctgagac cggagaaata agaactgctc aggtcatcgt agctgttctc
     2101 ggggcatcaa gttacacatg ggcagaggcc acctggtctc agcagcttga agactgggtg
     2161 atgagtcatg ttcgctgctt ccagtggttg ggtggcgttc ctgaacttgt tgttccggac
     2221 aatctgaaaa gcgccacatc cagggcatgt aagtatgatc ctgacgttaa ccctacctac
     2281 cagcagatgc ttgagcatta taatgtcgca gttttgcctg cgcggccacg taaaccgaaa
     2341 gataaagcca aagctgaagt tggcgttcag gttgttgaac gctggatcat ggcccgaatc
     2401 aggcatgaga tcttctacag ccttgcatcg cttaatcagc gcattcggga gttgctggaa
     2461 agactgaata acaaaataat gcagaagttg ggttattcac gtgcagaact cttcatccag
     2521 cttgataaac ccgcactgaa gcctcttcct gaagccagtt acagttacac cctggtgaag
     2581 aaagtcagag ttcatgccga ttaccacgtg gaaatcgaca aacattacta ctcggttcca
     2641 tgttcgctgt taggccagca actggaagca tggatctccg gagaactggt aagactcttc
     2701 aatcaggggc aggaggttgc tgtgcacccg cgcaagcgta cttatggcta cagtacccgc
     2761 aacgagcaca tgcctgaagc tcatcgacag catgccacct ggacgccaga gcgtcttctg
     2821 gaatgggcgg ggcacatagg cagtgaaact catagttatg tgcttcatat actgaactct
     2881 cgtccacatc cggaacaaag ctatcgcttc tgccttggac tcctgaacct tcataaaaaa
     2941 tacagtaaag ccagacttaa tgcagcatgt gcaagagctc tgaaaacaaa ggtatggcgt
     3001 ctgtcaggta ttaaatcgat cctggaaaaa ggtctggata aacaacctgt tcaggatcca
     3061 aaaccagatc tgttatccac gatggaacac gaaaacgtac gcggcagtga gtattaccac
     3121 tgatacggga tccaatgatg aatcatcttt acgaacaact gaccgcactt aaactcaccg
     3181 gcttccgtga tgcgcttaaa aagcaacttg ctcagccggg cacataccag gagctgggct
     3241 tcgaagaacg cctgtcatta ctgacagcag aagaactaac ctgccgtgaa aacaggaagg
     3301 cagagcgtct gatcaaacat gcacggttca gacttaatgc tgagttatca aagctggatt
     3361 atcgtaacaa tagagggctg gacagggccc tcatccgttc actcagtcag gga
//
LOCUS       DAJTUM010000214         3397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTUM010000214 DAJTUM010000000
VERSION     DAJTUM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(105..1814)
                     /locus_tag="OO471_004153"
     CDS             complement(105..1814)
                     /locus_tag="OO471_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6245171.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2246..2965)
                     /locus_tag="OO471_004154"
     CDS             complement(2246..2965)
                     /locus_tag="OO471_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6245172.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3090..3397)
                     /locus_tag="OO471_004155"
                     /pseudo
     CDS             complement(3090..3397)
                     /locus_tag="OO471_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1002 a          682 c          581 g         1132 t
ORIGIN      
        1 tgccaattga ggtggttgcc tgaaagttgg cagttacccg gtcactacac cttgttggag
       61 tgaacatatc aaaaacaggt aattacacaa tttaaattac agtctcataa aaaatgatta
      121 aatctattta ttatttcaat aaacgttttt ttcatcttct ttgaaaattg ttctgctata
      181 gtatccggtt ccagattgtt ctcttttatt attctacaat actctaatat tgtttctttg
      241 tctctattta ctacagatac aaatacacca ggaataccat ctttattagt tgcggacagc
      301 atttcgacca atcgtcttgt ggtaaaacta tagtttttta gaatattaca aaaggcacgt
      361 attgtttcgg tatggccatt ataaaatgcc ataaaaagtc ctgatgtacc gttgggattt
      421 tttatccata ataattcatc tatatattcc tggtgagggg ggagtatttt tagtatatct
      481 ccatacgcca taatggcctt tgtgtgccca ttttgaagtg ccataaacaa cccatgcttt
      541 actttcaata attctttaat ttgttcacta ctaagcattt tcttattaag cagacttccg
      601 aacatacgaa tactatctgc atgtccattc tgcaatgcaa aaaataaccc aggtaaatca
      661 tggctgtctt tcgcagctaa tagatataat ctcttatcgg aagttaacgg ggttttaaag
      721 actatttccc cataggcttt tattgtgtct acatgtccgt tcataaaaga catgcataaa
      781 cccggcacat tgtttgcccc taccgcagca agcaatgtaa gtattgtttc ttgactaaga
      841 ttgcacatat ttaatatctt accatatact tgaataatgt ctgaatatcc gttttgaagt
      901 gctatataaa atgcaggagt accatcagag cttctcgtgg ccagaaggtt caccagttta
      961 gattgagaaa tttcaccatt tttgtatttt tccatcagta taaataatac ttctgtcaga
     1021 ttctttgtta tcgcatgata tataactctg gaattgatca attcatctgg taaaagtttc
     1081 actgtgtttg ttagagggga attatgccta tcttcaaata ttacctgccc gggttgctcc
     1141 agaccataaa ttttccaata aaaatcatca aaaaaatcct tagcggataa ttgactcagt
     1201 tcgaaatggc tatcacaagt aaatacagta cgtttatgag taacagttcg attaggatcg
     1261 aaaaactgaa caacccattt ttcactttct tgtacgcgct ttattttcaa ccctaacgcc
     1321 atttggtggt ttaatgtgca tgctgataat gttctgacaa agtctccatt ttttttcatg
     1381 ttataaaaaa aattagtaat aactgttccc cactttttat tttccacaaa ataaatataa
     1441 gctggtgatt ttgaaaaatc ttccgttttt tccaaacata cattttttgc aatagcatca
     1501 ggggtgctaa gaagctgata gtttaccttc ccatttgttt ccataaaatt catttcccaa
     1561 taggaagcta ggtgacggca aactatttgt cttccatata tatcttttgc cactccattt
     1621 atatttacct ccacatttct cttttctgaa aaataaggtg ctgttttatt cttaaactga
     1681 attagttcac tggagtttga gaccctcata atagaacttg ttattgttct attatctctg
     1741 ttaacagaca tatctgttga taacgaattt tttgttgaaa atatactaga tgaaaatgtt
     1801 ttatttaacg gcatagaaga caaccataga gatgttataa tactattaga ttgcagttac
     1861 taatagtaaa agcctcctta caaggaggta gcttattcag aatgctccta taagatagcg
     1921 agctcgtcaa cgatacagat gatctgatta tattgtgcaa aaggagaaca ggaaaatagg
     1981 cactggaaaa gatgaaggcc tgatgggagc attaactaat gcaaaatccg acaggttaat
     2041 atcccgaaaa gcaatcaaac gactcaaaca ggagatgcac gctgaaatgg tggacaactg
     2101 ttcggatttc agatgagtag ctcagcagtt tatgagccga atgctctgtc acgggcagga
     2161 atcaggctgc atacgcagtg ccaaatctaa tcgtagttat tctaaccagt tttcaagcta
     2221 caaggtggtg tagctggcat cttctctact ctatcatcaa acgataaaat ggttcctctc
     2281 gtaacatttg ctcttgcctt tcacttccat ctcgatcgct tcgtagttca ttacgatagc
     2341 tgacatattt ccagtcttca ggtctaacat ctgacgccgg atactccccg ggagcgattt
     2401 tgcttcttaa tagattagac tcgagacaca ttataaattg tcgtatttta tgaagtaata
     2461 aagcactata ttgcgagcat tcctgatctg attttacata tagcgtaaac tgagctccta
     2521 tccccacacg agattgctga gaaactcgat tcatatcagt tatcttccat ttatctatag
     2581 gactgtcctc tgaaaataat aaaccagaca aaatttggaa tgccagagga acttgctccc
     2641 tagctatact aatatgaaac ttgtccccaa caaaatcccc cttactctga ggattttcac
     2701 gacatgcatg aataaacaca tctccactat ggttcacaat ctggaatcct tggtacatga
     2761 cagaaaaaga ttgacatata tcagagtaag ttggcacgtt cgatgctact ttgaaatagt
     2821 acgctgggta ttgccgtatt tggttgtaca atatattgaa gttatttttc aatctttcat
     2881 tcgcagaaag catctgcggt atttcagacc tcacaacatt caaagaatct aaatttagtt
     2941 ttagacaggg cttttttatg ggcatagaaa acgtcctcta taaaatagat atattatctt
     3001 atattgattg tttatcatct tttataaaac gatacattat atgtaatgta tgtaagcgta
     3061 agggtcctct ccgaacatgg tgggaaggct tagaacgcca tattcagttt cccatagtgg
     3121 aacatcactc ccagcttaaa gcgctcccgg ttgcggtagc ccttggcttt tattctcagc
     3181 agcctgatct tgctgttcag tgcttccgca tttccgtttg atacgccatg gcgcatggcg
     3241 ttcaggatac cgtacaacct tttccctatc gttttagcca cattcttcat catggggacg
     3301 tcactgttgg ccgcagccat ctctgccagt cgttgcgtct ttcctcgctc catggtctgc
     3361 tccagatatc ctttgccagt tctttcagcg cccagca
//
LOCUS       DAJTUM010000215         3394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTUM010000215 DAJTUM010000000
VERSION     DAJTUM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..442
                     /locus_tag="OO471_004156"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO471_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1027..1743
                     /locus_tag="OO471_004157"
     CDS             1027..1743
                     /locus_tag="OO471_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245173.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2295..2699
                     /locus_tag="OO471_004158"
     CDS             2295..2699
                     /locus_tag="OO471_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245174.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2762..3268
                     /locus_tag="OO471_004159"
                     /pseudo
     CDS             2762..3268
                     /locus_tag="OO471_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          938 a          714 c          776 g          966 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaatactgg attgtgggcc ataaatattc caaaaagata
      481 gcccgctccg tttattaccg gaacgggcga gagcatgaca ttgtctgtta ttataatgag
      541 actgctgatt ctacttattt ttaatttcaa gaaaagcgtt ttctacaaag aattatgaga
      601 tttaatgttt tgtttagaca atattatcat tctgttcaat aatgaaagtc ttcatcaaca
      661 acaagtaaaa acaacgtccc tctcatcgaa ccggacttgc agatttcccg catccggctc
      721 tcttgcaaca catcagacct tcacgttcgg gcggttatgg ggaaggtcca gcaagctgta
      781 tgagccctaa ctgtttgaat accagtagat taccagaaac ttctatcgat tgaagacatt
      841 accaactagc gttactaaat gttgaccaag ttttgttcac ttgtctggtt ttgatagtag
      901 cattctgcag agtattttga tatgataaat atgcattaac aatgatatta ttgttaagtt
      961 cctagcatga aaattaaaag atcaactttt atctcgaata ttttttacat tatcagttag
     1021 tttcttatga atgacaattc attgcttaga aattcttcat tattcatcgc ctatatggga
     1081 tgtgtagggt gggtcagtgc ttattcttat ggctggggaa cctcttttta ctatggattt
     1141 ccatggtggg ttgttggtgc aggtttggat gatgttgcca gaagtttact ctatgctatc
     1201 atagtaatgg gtatactttt taccggttgg gggattggta ttttattttt tcttcttata
     1261 aagaaaagaa gtaaaataca agacttgagt ttttttagac tattttttgc tattacccta
     1321 ctgtttttcc ctgtaatatt cgaactttta attctcaaac aatatttcat acttccatta
     1381 tcactttcat tcattattag ttcattggtt atttcaataa taattcgtat atatggacgg
     1441 atcttttctg tatcatgttt ttcagatatc ccttttgtta gagaacaccg cattaagtta
     1501 attatggctg gttttttggt ttatttttgg tttttttcct ttcttgttgg ctggtataaa
     1561 cctcaattaa aaaaagaata tcagatgctt tgttataata atagttggta ttatgttctt
     1621 gctcgttatg attcacggct cgttttatct agttcattta aagatgacag taacagattc
     1681 ttaatattta atacagaaca gtcgggattt tatgaaatca atgatgttta tgtcagaaag
     1741 taataatcct ctcccacaaa gtgaattaaa tgaatatata atgcgttcga agtatatgta
     1801 ttgagctgtc atactgagaa acaaacctta gggcagcaca attaaagcca gaaacaacta
     1861 tgcaagaaag ttactaaata agtgctcgga acagtaaaac actcaggggg gactcagtcc
     1921 ggattatgta atcagataaa ttgccaaaca tcacaaatca ccagccggac tgagtgatgc
     1981 cgagcattgc accaataccc cgtgatgaac gatgcctgat gcagaaagct ccctataaaa
     2041 caaagaacaa aaactacgac cggcgattca ccgtcatact gatgctgcat cgtggtgaca
     2101 ctgtctgtca ctttcatgtt gactgaacaa ctctgctgcg aagaaggtgt accgccggcg
     2161 ggatttgatt gtccggcgtc tagcttccac ttccctgatg gaactgaaag ttgttatggc
     2221 cggtcagctt gccctgtccg caggacatga ggtgctgtga cggacagcac cagtaacgga
     2281 aagtgacaat gaaaatgaat actgcgctga tagttgcact aatgtgtatg tggtatgcgg
     2341 tacccgctgc tgcaaaagag actttactgg ctatgccacg taattcaaca gaacattgct
     2401 atgcggagat taatgtgcac ggaccatatg gggtgtattt ccgagtggtc ccgcatcccc
     2461 ccgggggaaa gagctgggtc gaatgtaaca gtgactacta ttactctgat aaaccaccgg
     2521 gagtacagat actcggaacc cgtgcagggt gtcgggtata cggaatctgc gggaccacct
     2581 cgacactgca tgtggctgga agaggggttg tgtgtattaa aaatatatgc agtccacgtg
     2641 ggatgataat tcacaggata cggaaacgtc ctgtagtggc tgtaagcgat gagatgtgat
     2701 cttacccagc aatagtagac acgcggctaa gtgagtaaac tctcagtcag aggtgactca
     2761 catgacaaaa acagtatcaa ccagcaaaaa aacacgtaaa cagcattcgc ctgaattttg
     2821 cagtgaagcc ctgaagcttg ctgaacgcat cggtgttgct gccgcagccc gtgaactcag
     2881 cctgtatgaa tctcagctct atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc
     2941 tgagcgtgaa agtgaactgc ccgcctgaag cggcaactgg cggagcaggc agaggaactg
     3001 gctatcctcc aaaaggccgc gacatacttt gcgcagcggc tggtatacgt ggtgtcagcg
     3061 tcggacagga ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttgtcctcgc
     3121 ggcggccttt acccggtcaa aacagcgtta cggtgcacca cgcctgacgg atgaactgcg
     3181 tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct
     3241 gagggcaaag gcctcccgga agttcacgta taggaaattg aaaaaccaga cgataccgct
     3301 gtccacgcct tatgccacgt gagcttttct gcttttccct ccactccggt gttttctctc
     3361 tctgcatggt cgattcttat accgcaacag gctg
//
LOCUS       DAJTUM010000216         3336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTUM010000216 DAJTUM010000000
VERSION     DAJTUM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            274..578
                     /locus_tag="OO471_004160"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO471_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..2111
                     /locus_tag="OO471_004161"
     CDS             747..2111
                     /locus_tag="OO471_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6245175.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2161..2370
                     /locus_tag="OO471_004162"
                     /pseudo
     CDS             2161..2370
                     /locus_tag="OO471_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2464..2655)
                     /locus_tag="OO471_004163"
                     /pseudo
     CDS             complement(2464..2655)
                     /locus_tag="OO471_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2696..3193)
                     /locus_tag="OO471_004164"
                     /pseudo
     CDS             complement(2696..3193)
                     /locus_tag="OO471_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          819 a          738 c          790 g          989 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta attttctcag gaaaaacata aaattactga ctaaatattt attttttgaa
     2161 tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta tggatacagt
     2221 atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt tcagcttcat
     2281 ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat cctgtatgca
     2341 ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg cgatatatct
     2401 ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg tgatgtcaga
     2461 tgtccccagt aagactatgt tttcattgcg ctctatgaag ctgagtgagc gtaacgactg
     2521 gagctgcttc tgcggtgctc cggtggcgaa cgtgaagtca tactcttcga acattttcac
     2581 cgccgggaag gctgccattc gggtatacat cgcctgttta cgttgatgac gtgccagttt
     2641 ttcttcatga agcatgatgc tccaagtaaa cgttcaccga acaccgtatt gacgattatc
     2701 ctgatggtag caccaccttc catggcagga ggtcgtcaac tcggttggaa ggccattccg
     2761 gcagtacgct cagaatatgg cgcagatacg cttccggatc gataccgttc agacggcagg
     2821 tgccgatcag cccgtacagc agtgcaccac gctcgccgcc gtgatcgcta ccgaagaaca
     2881 cgtaattttt ctttccgaga cagactgcac gaagcgctct ttccgctgtg ttattgtccg
     2941 cctccgccag accgtcatca ctgtaataac agagggcgtc ccactgattc agtacatagc
     3001 tgaatgcttc gcccagtctg gattttttcg acagcgtgcc attcttctcc accatccatt
     3061 catgcagcga cgtcagtaac gctttgcttc gctgctgcct gactgcaaga cactctgact
     3121 ctggtaatcc ccgtatttca tcctcgatgg cgtacagttc actgattcgc ttcagggctt
     3181 cttctgccgt cgcacgcaag taatccgggg cccgctatgc cgcgctctat cgggcgggac
     3241 ggggctggcg ctttgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg
     3301 ctgccagatc atcgtttccg atggaagcat aataag
//
LOCUS       DAJTUM010000217         3310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTUM010000217 DAJTUM010000000
VERSION     DAJTUM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            440..1267
                     /locus_tag="OO471_004165"
     CDS             440..1267
                     /locus_tag="OO471_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6245176.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1311..2060
                     /locus_tag="OO471_004166"
     CDS             1311..2060
                     /locus_tag="OO471_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6245177.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2057..2626
                     /locus_tag="OO471_004167"
     CDS             2057..2626
                     /locus_tag="OO471_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245178.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2750..2992
                     /locus_tag="OO471_004168"
     CDS             2750..2992
                     /locus_tag="OO471_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6245179.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2996..3142
                     /locus_tag="OO471_004169"
     CDS             2996..3142
                     /locus_tag="OO471_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245180.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3151..>3310
                     /gene="xisR"
                     /locus_tag="OO471_004170"
     CDS             3151..>3310
                     /gene="xisR"
                     /locus_tag="OO471_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6245181.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          924 a          789 c          853 g          744 t
ORIGIN      
        1 agcgttttat cttcaacggg aatgagatat ggaaacgtgg agaatcaacc acagagtata
       61 cccgcgcaca ccagcgaaca atcagacgac tgcgcagact catcgccagg aaacagtata
      121 cagaaaaatg gttcagaaaa tatgacacat ggagcgccgg atattacgaa ctgatggcaa
      181 caaaaccatt ctgacgtaaa cgaaattaac catgacgcaa ttaaataagg caagccgaat
      241 acatcaggag gaccatgaac atttatttca gaatagttat atcactggca attatcgcat
      301 gtatttacgg attaatcgtt ccggccctca tatcaatgaa ggatacggta gcagtgattt
      361 ctggctttgc tctggcgtgt ctgaccccgc cctgcattta tgccatttat aagggtcttt
      421 ctttttctaa ggataaaaga tgaaaaaagt tattcctgct ttagcccttg ttctgctgac
      481 caccggactt gtcggttgcg atcgcgttga gcctggtaat gttggcatca aggtaaacaa
      541 actgggcgac gataaaggcg tcggagaagt ggtcggtgtt gggcgctact ggacgggatg
      601 gaacactgag gtttacatct tcccgacctt caaacaaatg aagacctacg atgagccatt
      661 cagcttccag atgagtgacg gtacaaccat cggctatcac atcggcgtgg cctacaaagt
      721 tgatccatcc aaagttacca cagtctttca gacctaccgc aaaggcgtgg acgacattac
      781 cgacaccgac ctgcgccaga agatagccga cgcactcaac cgactggcca gcaaaatgac
      841 caccgacaaa tttatcgacg gcggcaagtc tgagctgctg gatgcagctc ttaaagacat
      901 tcaggaagaa atgacgccca tcggtattca ggtaatgagc ctctcatatg tgggtaagcc
      961 ggagtacccg cctactgtta tcgacagcat taatgccaaa gtcacggcga accagaaaac
     1021 cctgcaatgc gagcaggaag taaaacagcg cgaagcggaa gccaacatgt tgcgcgcgga
     1081 agctgccgga caggcagatg cgattcgcac aaaagcccag gccgaagccg atgctattcg
     1141 tttacgcggt gaagctctgc gccagaaccc aaacgttatg gagctggaag ccatcaacaa
     1201 gtggaacggt acactgccgc aatacatgac cagcggtgcc aacacaccat ttattcaggt
     1261 taagtaatac atatgcccgg tattacacgc cggggtgtct ggagataaaa atgaatactg
     1321 taaccatcaa caacaaacag tttccggtaa tcgaatatcg cggtcagcgt gttgtgacgc
     1381 tggcgatgat tgatgaagtc caccagcgac cggaaggaac cgcgcgtgca gcattcaacc
     1441 gaaaccgtga gcatttcatc agcggtgtgg attatgccga attaggtgcg gacgtaatac
     1501 gtacggacct cccggaagga acattctcta aatttgcacc atcggggatt gtactttttg
     1561 aatcaggtta cctgatgttg acgaagccat tcaatgacgc ccttgcctgg caggttcagc
     1621 gcgaactggt taacagctac ttccgcactc gcacaccgct aacggaaatc gagatgatcg
     1681 ctgcgatagc cgccgacgct gttcgtcaac agaagcgcct gaatcatgtg gaagaacagc
     1741 tcgaaacagt cacagaagcg gtggaaacca tcaaacgcgg aaatatgcgc gccggatatg
     1801 tcggttaccg ccaggtggta gccaaaagtg gaatgagtga caccaagtgt cggaatctgg
     1861 tgaacgcata tcaaattcct acagacaccc acgaattcat gactccggac ggtctgttgt
     1921 ctcgcagggc tatcgtcgag cttgagccat ttatggcggc gttccaccaa atgatgtcag
     1981 aggctgaccc acgcggcaca cgctggtatc acccgaagat ggggcttttc caggtgatcg
     2041 gatgggagga taaagcatga tcatccagtc aaaacttatt cgcgccgctc tggtgtgcgc
     2101 tgctaaaaac gacgttcgtt attacctgaa cggtcttcac atcacgccga aacatattga
     2161 ggcaaccaat ggttccgtgg cactgcgcat ggctcacggc atccggacga agaaaaacat
     2221 cattgtccag ttcgaagggg gcgtcccggc caaagccgaa acgacagagc tgatttttag
     2281 taaagagccg atcgctgttc atcgcgacca gtttcagcga cgactgtcca ttaccggcat
     2341 taaactggtg gacggttgtt ttccggattt ggatcgcatc attccgaaaa agtttgaccg
     2401 ctgtactcac ccggtgctcc aggcgggtta cctgagttat ccagagaaga tgtttggtcg
     2461 tgagcgtaaa tttattcccg tccagttacg tccctccggt gacgggcaag cggtcagaat
     2521 tcagtttgat tccatcatca actcaatgta tggcaatcct gaatttgttg tgatgccttg
     2581 tcgtgatcat ggcgatttca acgtggctca ggagcatccg gaatgaaaat cgaataccag
     2641 gacgccgcag gaggtgaatc aatgagctgg cctgatgcaa tcgtaactct ggggatggta
     2701 ttcgcagtag cgtttgttgt gtactcgatt tgtcgatggg gataaccaca tgttcgcttt
     2761 gattcaacgc gggcagatat acacagacag agccggatac ccagtagaaa ttactcgcag
     2821 tactgagcac tcagtgttct ttcggaggat ggacggacgt accgggcgag tacgcatcgg
     2881 tgaattcagt agcctgttcg agcatattga ccacctggaa tatcacaaga ttctggctga
     2941 aacagaacaa gaaaagcacc tgaagaaatt acgcgcaatg caaaggaagt aaaaaatgaa
     3001 taaagcgttt gagctatggg tccaccagcg ttacggcaac cactacgacc tgacgcgaga
     3061 tgttgacggt ttctactgtc gtgaaattgt gaaacgaatg tttgaggtct ggtgccactg
     3121 ccgtgggctg agtgttgtgt gaggtaatgc atgggcaatg tgattcaact ggctcccaat
     3181 gaatgggttt gtgaaagcgt tctaatcgca attaccgggc tcaaaccagg cacaattctt
     3241 cggacccgga aagaatgctg gatggttgga agagagtata ttcacgtatc accagacggt
     3301 aatccaaagc
//
LOCUS       DAJTUM010000218         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTUM010000218 DAJTUM010000000
VERSION     DAJTUM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO471_004171"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO471_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6245182.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO471_004172"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO471_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTUM010000219         3130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTUM010000219 DAJTUM010000000
VERSION     DAJTUM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..238
                     /locus_tag="OO471_004173"
     CDS             <1..238
                     /locus_tag="OO471_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6245183.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO471_004174"
     CDS             248..886
                     /locus_tag="OO471_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6245184.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO471_004175"
     CDS             889..2052
                     /locus_tag="OO471_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6245185.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3130
                     /locus_tag="OO471_004176"
     CDS             2136..>3130
                     /locus_tag="OO471_004176"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6245186.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPL"
BASE COUNT          705 a          738 c          891 g          796 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat
//
LOCUS       DAJTUM010000220         3092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTUM010000220 DAJTUM010000000
VERSION     DAJTUM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..689)
                     /gene="ipaH9.8"
                     /locus_tag="OO471_004177"
     CDS             complement(<1..689)
                     /gene="ipaH9.8"
                     /locus_tag="OO471_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6245187.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSS"
     gene            complement(951..1046)
                     /locus_tag="OO471_004178"
                     /pseudo
     CDS             complement(951..1046)
                     /locus_tag="OO471_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1082..1387)
                     /gene="ccdB"
                     /locus_tag="OO471_004179"
     CDS             complement(1082..1387)
                     /gene="ccdB"
                     /locus_tag="OO471_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6245188.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1389..1607)
                     /gene="ccdA"
                     /locus_tag="OO471_004180"
     CDS             complement(1389..1607)
                     /gene="ccdA"
                     /locus_tag="OO471_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6245189.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2143..3092)
                     /locus_tag="OO471_004181"
                     /pseudo
     CDS             complement(2143..3092)
                     /locus_tag="OO471_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          772 a          748 c          747 g          825 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt atcactaata
       61 tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact tatctgattt
      121 ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc tggtagctca
      181 gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga tggtaaggag
      241 actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc tggcaggttt
      301 tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac gggcaattct
      361 gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc aggtaggtta
      421 gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt gtcaggtagc
      481 gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag acattcttta
      541 agtcgggaga cagcctcatc acgctcttca ccggggagcg cttgtttttc ccatttatcc
      601 catgctgaaa agtaatcagc atatgtaccg gaaatagtgt tataaaaaga attttggggc
      661 aatgaaaagt tattatttat cggtaacatg atgggcatcc agttaaagcc ggtggacaaa
      721 tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg aaatgaactt
      781 cagcactttt ttattataat aaggataaac aaaattggct gtaaaaaaac gatactgttt
      841 cagttaaccg tgaatggcct gtttgcaccg tattgttctt gtttttcggt tagttaaaca
      901 tcgtacttca tatttgaacg gtaagcggct cgtcagaacc gtattgatat ttactgagag
      961 gtcagatcaa ctttccaagg caacagatcg cgtacccggt ttgccggcca gtcctggata
     1021 tgctcaatga cgtaacgcag ccattttgtc aacgccacga tttttgaccc tagcctgaca
     1081 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     1141 gatcagccac ttcttccccg ataacgaaga tcggcacact ggccatatca gtggtcatca
     1201 tgcgccagct ttcatccccg acatgcacca ccgggtaaag ctcacgggag actttatctg
     1261 acagcaggcg ggcactggcc agggggatca ccatccgtcg tccgggcgtg tcaataatgt
     1321 cactctgtac atccacaaac agacgataac gactctctct tttataggcg taaaccttaa
     1381 actgcatttc accagtccct gttctcatcg gcaaaagagc cgttcatttc aataaaacgg
     1441 gcgatctcag ccatcccctg ctgattttca gcctgccagc gttccgcacg cagacggcgg
     1501 gcctcttttt gcatggccgt atttaccagg cctgagatat tcacgtttgc ggatttaagt
     1561 aactgatagc tgtcactgtc tatagtgaca gtaatacgct gcttcatgat aagccctcaa
     1621 aaacatactt atgatatgta tatgagtata tattaatggt acaaaccatc aatatccata
     1681 aaaagtacac cattaattcg ctataagttt ttaatacaat tttttctgct gcgatttttt
     1741 tatactataa ttagcatact tataacaccg taagtaaatt acatgaaagc aggcacagcg
     1801 tgatccgatt tttctgcgta taacgatttt ccggaagtgt aatacggttg cgccccgggc
     1861 cgggcaccct tttctgttgt tgtgtacggg cccacagaga atggtaccgg aggcgggctc
     1921 agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta ctgacacggg tattggtgac
     1981 tggtgatgaa gtactgcccc gggtatgatt tcaggctgat tcaggttctg attctctgtc
     2041 agtccgcgtg gttgctggcc tggctgattt cctgttgtca ggcgttcaac aaccgactgc
     2101 aggttatagc ccggttcacg aacacctgac ggcaggtatc actcacccgg tgaaggtaaa
     2161 tccctcaaga ggcagcaact ctgccagtcg ctcctccggc cattccggca gacgcgtcag
     2221 gacgtccgtc agccaggcat gtggctccag accattacgt tttgctgttt ccagaaggct
     2281 cattatctgt gccgcccggt ttccggccat cagtgaaccg gcgaacagcc acgctttccg
     2341 tcccataaca accgggcgaa tggcacgctc gcagatattg ttgtccaggg gcacagctcc
     2401 gtcacccagg aagcagctca gttcatcgcg ccggttcaga atatagttaa tcgccttatt
     2461 taatggcccg tcgggcggac agcacggctc ctgctcttca agccaggcaa acaggtcatt
     2521 cactatcggc cgggagtacc gttatcgcca ctgccgtatt ttttccacag ggcgctcacg
     2581 gatgagtttc tcaatgcggt aaagacccgc gatttttctc agctccacgc ctgcacgggg
     2641 ctccctgctg gccttataca gatttgcgaa gccgcgccct gcgtgactcc agcaacaggc
     2701 tgatgtgatg ccggggtgat tatccgccag agatttgtac acgctgtatc cgtcactcac
     2761 cagcgtactg ccacaccagc actgcagcca ggtctcaggg tatctgaggg cccgtccggt
     2821 ctgcgagtca aaacagacga ccggcggacc gcttctttca ccgctgacgt aagcacacag
     2881 ccagccggag caggatttac cgcctttccg ggtatcaagg agcctcaggg acgtttcatc
     2941 tgcatgaata acatcccgtg tcagaagctc atggcgtagc agtttcgcca gcggggccag
     3001 tgcagctccg gcaacaccga ccatatccgc cattgtactg acggggagct ccacacccat
     3061 tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTUM010000221         3071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTUM010000221 DAJTUM010000000
VERSION     DAJTUM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            52..1083
                     /gene="ynaI"
                     /locus_tag="OO471_004182"
     CDS             52..1083
                     /gene="ynaI"
                     /locus_tag="OO471_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6245190.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1134..2747)
                     /gene="mppA"
                     /locus_tag="OO471_004183"
     CDS             complement(1134..2747)
                     /gene="mppA"
                     /locus_tag="OO471_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6245191.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          808 a          705 c          679 g          879 t
ORIGIN      
        1 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
       61 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
      121 attctgatga gcttttggtt tcgccgtgga aatcgtaaaa gaaaaggatt tttattccat
      181 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat cagtaattat
      241 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
      301 tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa
      361 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
      421 atcaccatta ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
      481 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
      541 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
      601 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
      661 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
      721 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
      781 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
      841 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac
      901 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt
      961 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
     1021 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acaggggcgc
     1081 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
     1141 ttcacaatat acatagtccg actgtacgct acatcttcag ggttattaat gggataacct
     1201 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
     1261 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
     1321 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
     1381 cctgaatgca ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
     1441 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
     1501 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
     1561 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
     1621 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
     1681 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa
     1741 tgccacgctg gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc
     1801 gtcatactta atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag
     1861 gcataataat aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac
     1921 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
     1981 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt
     2041 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
     2101 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
     2161 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
     2221 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
     2281 agctgatcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
     2341 gcggcaaacc atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag
     2401 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
     2461 aaagtccaga tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc
     2521 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
     2581 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
     2641 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
     2701 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
     2761 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
     2821 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
     2881 ttagtgtaaa taatagacgc caaaatgtgg caaagcacat agaacaccga acactacgtt
     2941 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
     3001 gaagaataaa aaataaccaa aacgttgaaa ggaactgaga atacgttaag agaagaatta
     3061 aattaatgta t
//
LOCUS       DAJTUM010000222         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTUM010000222 DAJTUM010000000
VERSION     DAJTUM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..498
                     /locus_tag="OO471_004184"
     CDS             <1..498
                     /locus_tag="OO471_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6245192.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO471_004185"
     CDS             578..1273
                     /locus_tag="OO471_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6245193.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="OO471_004186"
     CDS             1302..>3069
                     /locus_tag="OO471_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6245194.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAJTUM010000223         3065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTUM010000223 DAJTUM010000000
VERSION     DAJTUM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..197
                     /locus_tag="OO471_004187"
     CDS             <1..197
                     /locus_tag="OO471_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6245195.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO471_004188"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO471_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6245196.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO471_004189"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO471_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6245197.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO471_004190"
     CDS             1821..2576
                     /locus_tag="OO471_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6245198.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="OO471_004191"
     CDS             2977..>3065
                     /locus_tag="OO471_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6245199.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          797 a          683 c          727 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DAJTUM010000224         3055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTUM010000224 DAJTUM010000000
VERSION     DAJTUM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..776
                     /locus_tag="OO471_004192"
     CDS             <1..776
                     /locus_tag="OO471_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6245200.1"
                     /translation="KPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIK
                     VPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRR
                     VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRK
                     MRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRR
                     YAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1125..2297
                     /locus_tag="OO471_004193"
     CDS             1125..2297
                     /locus_tag="OO471_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6245201.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKESFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2351..2974)
                     /locus_tag="OO471_004194"
     CDS             complement(2351..2974)
                     /locus_tag="OO471_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245202.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          947 a          643 c          605 g          860 t
ORIGIN      
        1 ggaaacctgt tccagaatac acgccaaaac cagcgtccgt tgcaacgaag gctacgcatc
       61 tttcatttat aaaggcccta ctaagagccg cagagcgtga atggaaaatg ctggataagg
      121 caccaattat taaagtgcct caaccaaaga ataaacggat ccgctggctg gagccccatg
      181 aagcacaaag gctgattgat gaatgtccgg agccattaaa gtctgttgtt gaatttgcac
      241 tggcaacagg cttaagacgc tcgaacatca tcaaccttga atggcaacaa atagatatgc
      301 agcgccgggt ggcatggata aacccggaag agagtaaatc aaaccgcgca attggcgttg
      361 cgctgaatga tactgcatgt cgcgtattga aaaaacaaat cgggaatcat caccgttggg
      421 tatttgtgta caaggaaagc tgtaccaaac cagacggaac gaaagcgcca acggtcagga
      481 agatgcggta tgacgcaaac acagcctgga aagcggcgct gagacgagca ggtattgatg
      541 atttcagatt tcacgacttg agacacacct gggcaagttg gctggttcaa gccggagtcc
      601 cgttgtcagt gttacaggaa atgggaggct gggagtctat cgaaatggtt cgtcgatatg
      661 ctcaccttgc acctaatcac cttaccgaac acgcacggca aatagactcg atcctgaacc
      721 catcggtccc aaattcgtcc cagtcaaaaa ataaggaagg tactaatgat gtgtaactta
      781 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaatctgt
      841 agcaccaatc ataactatct gttttagcaa gcattaaccg cattcactaa gctatagttg
      901 atgacacaaa cagaaagttg atgcatgatg ttgccatata tatgtcacaa atacggcaca
      961 acgatcttca aacatatagc cacacattca cagaagagca caaagccttg caatccagtg
     1021 caaagcttgg tgtgtctccg ttttgtttca tcagttacac ataactatat gatcattaac
     1081 taaaagtcag tatacttgga gaaattttaa ttgcgtaata gagcatgcat atgattgaaa
     1141 ttaacagttt attattaata acatccgtga tattgatgtc gttattagct gtggggttat
     1201 ttgataaaat ttccccaata aatcttgttg agcacggcag gaataatcaa atagatggaa
     1261 tgcgggggtt cttagcaatt ttcgtgctta ttcatcacgc agcaatttgg aatggatact
     1321 tgtcatctgg agtatgggaa gcaccttcat caaatctgtt agcaaattta ggccaagttg
     1381 gtgtgtcatt cttttttatg attactggtt atctgttctt ttcaaagatt atctctggag
     1441 atcaggactg gacaaggctt tatgtatcaa gattactacg attaacccca atgttcatag
     1501 ttagcctatg tctcattttt atcattgtag gttttaagtc tggatggaga atgcaggtat
     1561 ccacagaaga gctttttgtg tcaataatga agtggttgcc attcactgcg ctaggtatgc
     1621 cgaacattaa tgacgtaaaa gagtcattta ctatcaatgc cgctgtaaca tggacacttg
     1681 tatatgaatg gttcttttat ttttctcttc cggtaatttc cgcgctcata aaaagaaaag
     1741 tcagtattta catggttatg attagcgcaa tatcgctatt tgttttcatt ttatttttca
     1801 gtaaaataca catagcatca tttctttttg ggctcctggc atttttgcta aataaatcaa
     1861 agatagtcaa tggaattgcc aaagcaaaag taacaccaat tataataact gcgataatgg
     1921 tttttgaaat gacgtatttc aaaacaactt acgcaccgct accactgatt ctttgtggaa
     1981 taacatttat cattatcgca tcaggttgtg atttgtatgg aatattaaga ttaaatataa
     2041 ccagaaagct aggagaaact acctacagcg tttaccttct gcatgggata ttcctttatt
     2101 gcctaatgac gtggattatt cctaataatt acactgaaaa tacatttatc atactggtaa
     2161 gcacaactgc atttcttatt acgttcacat catgcctaac ttttaaatta atagagacgc
     2221 cattcatcaa attaacaaaa caaaccacaa ctttagtaaa agaattaata cccacattaa
     2281 caaacaacaa tcaataaaca ataaacataa gccgccctac atatatgtcc aaatggggcg
     2341 gcgaatgtat ttatattagt ctggtaatgc taaatgcaaa cgaactggag attcctgccc
     2401 cgctaagcgt attgcgcagg gtcaccctaa atgtatctac gccaatataa gtaacatcac
     2461 aactaactga atcaggcgca gatataggtg ttatctgcac aatataaccg ctagcaactg
     2521 ctgcgagcgg atgagaaaag ctaacatcat acacacctgt agaaaccacg ttaaccgatg
     2581 aaacaccagt agcaacgtcg atactaccat tggcgttaat ttttcccgag cgtgtgactg
     2641 acaatgcaga agccgatgtt accttactgg ttgtttctgt aaaaacacac ccaatgtcaa
     2701 ctacctcgca attagctcct gttatccagt atggtcgttc tgcacttggc tgataatccc
     2761 ctgcgctctg gaacgttgtg ccgttgagta tgactgtaac ttttccccca cctgagcttg
     2821 ttacttgaat attcgtgtgc gtgtatcttg cactgctcac gcgatgaaaa tttccaccgt
     2881 ttataaccac agttacaggg cgcgtcccag tattatctat agcaaggtct gcccccccgg
     2941 cattagcctc aaagtatggg ttgtttaata ccaaagcgca cgaaccgtta aggccattta
     3001 tagctaattg cgcaccacct gtactggaat ccccctgagt accatttcct tcaca
//
LOCUS       DAJTUM010000225         2952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTUM010000225 DAJTUM010000000
VERSION     DAJTUM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OO471_004195"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OO471_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6245203.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO471_004196"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO471_004196"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6245204.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..>2952
                     /locus_tag="OO471_004197"
     CDS             2604..>2952
                     /locus_tag="OO471_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6245205.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          701 c          779 g          813 t
ORIGIN      
        1 aaaggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg ggggcgaggt gctgtatgct
      841 gatatggggc actttggtaa gttctctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aa
//
LOCUS       DAJTUM010000226         2939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTUM010000226 DAJTUM010000000
VERSION     DAJTUM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..2743
                     /locus_tag="OO471_004198"
     rRNA            <1..2743
                     /locus_tag="OO471_004198"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2839..>2939
                     /gene="rrf"
                     /locus_tag="OO471_004199"
     rRNA            2839..>2939
                     /gene="rrf"
                     /locus_tag="OO471_004199"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          771 a          651 c          923 g          594 t
ORIGIN      
        1 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
       61 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
      121 agtgtgtgtg ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac
      181 acaaaaatgc acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
      241 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
      301 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
      361 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
      421 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
      481 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
      541 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
      601 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
      661 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
      721 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
      781 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
      841 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
      901 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
      961 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
     1021 gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta
     1081 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     1141 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     1201 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     1261 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     1321 ggttttccag gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc
     1381 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     1441 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     1501 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg
     1561 aagcgacttg ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     1621 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     1681 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
     1741 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     1801 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     1861 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     1921 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     1981 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     2041 tggacccgtg atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     2101 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     2161 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     2221 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     2281 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     2341 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     2401 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     2461 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     2521 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     2581 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
     2641 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     2701 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt
     2761 ttggcggatt gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     2821 ctgataaaac agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg
     2881 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagt
//
LOCUS       DAJTUM010000227         2876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTUM010000227 DAJTUM010000000
VERSION     DAJTUM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..290)
                     /locus_tag="OO471_004200"
     CDS             complement(<1..290)
                     /locus_tag="OO471_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6245206.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIA"
     gene            complement(337..1056)
                     /gene="yehT"
                     /locus_tag="OO471_004201"
     CDS             complement(337..1056)
                     /gene="yehT"
                     /locus_tag="OO471_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6245207.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1053..2738)
                     /gene="btsS"
                     /locus_tag="OO471_004202"
     CDS             complement(1053..2738)
                     /gene="btsS"
                     /locus_tag="OO471_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6245208.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          744 a          770 c          653 g          709 t
ORIGIN      
        1 aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt
       61 gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgacgac
      121 aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg
      181 atctgttccg cggtggcttc gacattatcc agcgccagaa tacgcaccag gtcattatta
      241 ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttatcat gaggccttca
      301 ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa
      361 gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc
      421 ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca gatactgacg
      481 atggcagcgc agtagtggtg tacgactttc caaggtacgt aatgtcaatt cggtaaagcc
      541 ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga caaatgccac
      601 atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa atttcagcgc
      661 ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac gcaatcgcgc
      721 cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa aggcatgttc
      781 ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat acgggcgatg
      841 ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag
      901 aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg cgtttgaaca
      961 ctctccaacg atttcaatat cgctctgctc ctgcaaaaat atacgcaggt tctcccgtgc
     1021 taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagtcgt
     1081 aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata gtcatcgcca
     1141 aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc
     1201 ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg
     1261 gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt
     1321 tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata
     1381 gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc
     1441 acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa gtttttgcgg
     1501 aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg gcggatcacc
     1561 gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc gtgaagcagt
     1621 ttgatttctg actgggtgag catcgctttt tgccgctcat attgccctgc aagaatctgc
     1681 gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact gaataaccgg
     1741 tttttggctt catacaattt gatggtgccc atcacgcgct gattttcgcc acgcaacgga
     1801 attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg
     1861 ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta agtcgaagaa
     1921 atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag caatttctct
     1981 cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagtacctg agccactttc
     2041 atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc tgccactttc
     2101 agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat
     2161 atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggcgc agcaatatta
     2221 ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg caaggatgat cagcatttgc
     2281 accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagtg
     2341 cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc aacgatggtt
     2401 gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa gccgccggtc
     2461 agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcgcctatc
     2521 gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag
     2581 aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt gacctgcatt
     2641 aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc
     2701 tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca cacttccctt
     2761 taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc agaaaaatga
     2821 gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatag
//
LOCUS       DAJTUM010000228         2807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTUM010000228 DAJTUM010000000
VERSION     DAJTUM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            6..81
                     /locus_tag="OO471_004203"
     tRNA            6..81
                     /locus_tag="OO471_004203"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO471_004204"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO471_004204"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO471_004205"
     CDS             complement(299..1057)
                     /locus_tag="OO471_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6245209.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO471_004206"
     CDS             complement(1065..2168)
                     /locus_tag="OO471_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6245210.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="OO471_004207"
     CDS             complement(2178..>2807)
                     /locus_tag="OO471_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6245211.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAJTUM010000229         2793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTUM010000229 DAJTUM010000000
VERSION     DAJTUM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            624..1223
                     /locus_tag="OO471_004208"
     CDS             624..1223
                     /locus_tag="OO471_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6245212.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1595..1996
                     /locus_tag="OO471_004209"
     CDS             1595..1996
                     /locus_tag="OO471_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245213.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            1984..2418
                     /locus_tag="OO471_004210"
     CDS             1984..2418
                     /locus_tag="OO471_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR6245214.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          707 a          663 c          711 g          712 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag acttttggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggctgttg
     1561 cctgcgtgtc gtctggcggc tgaggtatat gactatgtac gcaaaatcct ttatcgctct
     1621 tgatggcaac ggacgtctga cgggcgcccg tactgcacag gccgcacctt atgctaacta
     1681 cacctgccac ttgtgtggca gtgcactcag ataccatccg caatacgaaa ctgaacttcc
     1741 ctggtttgaa cacactgacg acaggctgac agagcacggt caacagtgcc cttatgtcag
     1801 gccggagcgc agagaaatac agttgattaa acgtctgcag caattcgtac cggatgcctt
     1861 acccgtggtg cgtaaagcca gctggcactg cagacaatgt caccacgatt attatgggga
     1921 gcagtactgc acacactgcc agaccggagg attcagtatt ccccggacaa ctcaggagga
     1981 aatatgcgaa ttctgaactg ttatatggcg aatgacagca aaggccattt tgttacggcg
     2041 aaagaagctg cgaagcacaa ccgacaggac gttttgtgct gtgtgtcctg tggatgcccg
     2101 ttaacacttc agcggggcaa tgacggacaa ccaccgtggt ttgaacatga ccagatgact
     2161 gtcgctgaaa aaatcctgct gcgatgtacc tggcttgacc cggcagagaa agaggcccgt
     2221 cgtttgcatc tgcagggcat gacggttccg gattatacgg tgaaggtgag aaagtggttt
     2281 tgtgtgatgt gtgacgaaga ttatgagggg gaaaagtgct gcccacgctg cggtaccggg
     2341 gtatacagca gggcgtgggg gcggcaggag gtgccgtcgg aagatgccag ggctgataat
     2401 ccgttacaga ggctgtaatg gttgcctccg gagcagtttg cggtgatgcc tttcccggga
     2461 atggattgcc agtgcgggga ctgtggagct taattccggt gttggtggcc tttccgttga
     2521 ttttatgcca acagccccct gtgactgaca ggctgcctcg tcattccatt cgtatctcca
     2581 gtaacagggg gttgtattgt attttattgt gccggttcag gtgtgcgttt cccggcgcgt
     2641 ctgcaccggc ttaaccaaat tcaacaggga taaaatagtg gtaagcgtac agcctgaacc
     2701 gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt agtgggaacc
     2761 aaagtgtcag atatgcagaa aaatgtgact ccc
//
LOCUS       DAJTUM010000230         2711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTUM010000230 DAJTUM010000000
VERSION     DAJTUM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            50..2572
                     /locus_tag="OO471_004211"
     CDS             50..2572
                     /locus_tag="OO471_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6245215.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2711
                     /locus_tag="OO471_004212"
     CDS             2583..>2711
                     /locus_tag="OO471_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6245216.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          677 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc g
//
LOCUS       DAJTUM010000231         2679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTUM010000231 DAJTUM010000000
VERSION     DAJTUM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO471_004213"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO471_004213"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6245217.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="OO471_004214"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="OO471_004214"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6245218.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          637 a          678 c          768 g          596 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcg
//
LOCUS       DAJTUM010000232         2606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTUM010000232 DAJTUM010000000
VERSION     DAJTUM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..261)
                     /locus_tag="OO471_004215"
     CDS             complement(<1..261)
                     /locus_tag="OO471_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6245219.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFA"
     gene            454..957
                     /locus_tag="OO471_004216"
     CDS             454..957
                     /locus_tag="OO471_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6245220.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1003..1419)
                     /gene="rraB"
                     /locus_tag="OO471_004217"
     CDS             complement(1003..1419)
                     /gene="rraB"
                     /locus_tag="OO471_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6245221.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1581..2594
                     /gene="argF"
                     /locus_tag="OO471_004218"
     CDS             1581..2594
                     /gene="argF"
                     /locus_tag="OO471_004218"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6245222.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          736 a          606 c          598 g          666 t
ORIGIN      
        1 tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa acattcccac cggtaattcc
       61 ataagaaaat cgttgtccgt taacttccat attagcataa agataacgct tacatttcat
      121 taatgcccat ggtaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca
      181 aataagaaaa tatctctcac ctgtaccatg aaaaacaaaa gaatgggacg gaacatccat
      241 ttcattaata acttgagcca taaaaatccc ctgaattaaa aaatccattg atataattta
      301 tagatacaga cagtaaaatg ttctgccacg ttacaatact cttatcagta ataggctggc
      361 aaaaacttta ttgctttata agacgcttgc gcacgtaaga agagagtggt attaaacaaa
      421 tgtataatta ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct
      481 gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat ccgtcaggtt
      541 tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc gaatctcgac
      601 gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga atacaatggc
      661 gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga catttgcgaa
      721 ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa aaaactggcc
      781 ttaatggcga tggagcaggc acgagagatg ggcttcaaac gctgctatct ggaaacgacc
      841 gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca tatcgactat
      901 gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga actctaaaaa
      961 aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc gaaccccgtc
     1021 atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat cttcaaagta
     1081 agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca gttgttcaac
     1141 ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc agcaaatcac
     1201 gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac cgagtttaaa
     1261 cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat ggtgttcgat
     1321 ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa tcaaacgtgt
     1381 ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct cttaatgtcc
     1441 tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg acaaagattt
     1501 atgctttaga cttgcaaatg aataataata cacataaatt gagttttaat tcattgaggc
     1561 gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg aaattactcg
     1621 atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg aaagctgata
     1681 agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc atcttcgaaa
     1741 aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag ggggctcgcg
     1801 ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt aaagacactg
     1861 cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt caggagattg
     1921 tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc aatgagtttc
     1981 accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc ggtaaagcat
     2041 tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc aattcgatgc
     2101 tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag gcatgctggc
     2161 cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc gggaatatta
     2221 cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat accgatgtat
     2281 gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta ctgcgtgatt
     2341 atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa ttcctccatt
     2401 gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg gaagagtttg
     2461 gcctacatgg cggaatggaa gtgactgatg aggtcttcga atctgccgcc agcattgtat
     2521 ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg acgctcagta
     2581 aattaaataa ttaatatcgc tataaa
//
LOCUS       DAJTUM010000233         2575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTUM010000233 DAJTUM010000000
VERSION     DAJTUM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..567
                     /locus_tag="OO471_004219"
     CDS             <1..567
                     /locus_tag="OO471_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6245223.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="OO471_004220"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="OO471_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6245224.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OO471_004221"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OO471_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6245225.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="OO471_004222"
     CDS             2169..2405
                     /locus_tag="OO471_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6245226.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2444..2530)
                     /locus_tag="OO471_004223"
     tRNA            complement(2444..2530)
                     /locus_tag="OO471_004223"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2494..2496),aa:Leu,seq:cag)
BASE COUNT          655 a          572 c          662 g          686 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     2521 ccaccttcgc acagtcatct tacttttttt gatatcgcct cgtttggtgc gaggg
//
LOCUS       DAJTUM010000234         2562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTUM010000234 DAJTUM010000000
VERSION     DAJTUM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..771
                     /gene="lsrF"
                     /locus_tag="OO471_004224"
     CDS             <1..771
                     /gene="lsrF"
                     /locus_tag="OO471_004224"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6245227.1"
                     /translation="MQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAPLF
                     EHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCA
                     VAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAA
                     EMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMG
                     RNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            795..1085
                     /gene="lsrG"
                     /locus_tag="OO471_004225"
     CDS             795..1085
                     /gene="lsrG"
                     /locus_tag="OO471_004225"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6245228.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1125..1883
                     /gene="tam"
                     /locus_tag="OO471_004226"
     CDS             1125..1883
                     /gene="tam"
                     /locus_tag="OO471_004226"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6245229.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1887..>2562)
                     /locus_tag="OO471_004227"
     CDS             complement(1887..>2562)
                     /locus_tag="OO471_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245230.1"
                     /translation="EARKLWGQLMIASRSLLREVKTTLPDSASVREFARLQIAFAHCL
                     RMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRILLIMGEWLAVQRRNGQLSDILFI
                     SLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLFCIMLPFALVVDLHYMTPFI
                     SVLISYTFISLDCLAEELEDPFGTENNDLPLDAICNAIEIDLLQMNDEAEIPAKILPD
                     RHYQLT"
BASE COUNT          652 a          603 c          685 g          622 t
ORIGIN      
        1 atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt gatgctggcc
       61 tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga tataaatatc
      121 gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta
      181 gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa ctctattctg
      241 gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt
      301 tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc gatcaaaaat
      361 attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc
      421 gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct
      481 gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga acggattgtt
      541 gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga gcgcgagacg
      601 ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat ggggcgtaat
      661 attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt ggttcaccat
      721 aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta actgcggatt
      781 taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa gacaaggttg
      841 acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa gaaggtaatt
      901 tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt tacgaagcct
      961 ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa acctgtgtcg
     1021 cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat gggttgatgc
     1081 cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct gactggaacc
     1141 cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag ctgcttgcca
     1201 gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca ggtaacagca
     1261 ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac tcgtctccgg
     1321 cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa gcagacatcc
     1381 gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca ctgcaatggc
     1441 tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg cagggcgtgc
     1501 tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg cgcgaagttg
     1561 cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt catgcttact
     1621 acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact tactatcacc
     1681 agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta cgtccgtggt
     1741 tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat cagatgctgg
     1801 aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg cgtctgttta
     1861 ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc gggaagaatt
     1921 tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat agcgttgcag
     1981 atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag ttcttccgcc
     2041 agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg cgtcatgtaa
     2101 tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac ggtacgatgc
     2161 aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca gcctgccaga
     2221 accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc tgaaagctgt
     2281 ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg gttagctgga
     2341 gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc cagcacttcc
     2401 gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat ttgcagcctg
     2461 gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg cagtaacgac
     2521 cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt ca
//
LOCUS       DAJTUM010000235         2547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTUM010000235 DAJTUM010000000
VERSION     DAJTUM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..98
                     /locus_tag="OO471_004228"
     CDS             <1..98
                     /locus_tag="OO471_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6245231.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO471_004229"
     CDS             156..512
                     /locus_tag="OO471_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245232.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="OO471_004230"
     CDS             605..823
                     /locus_tag="OO471_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6245233.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="OO471_004231"
     CDS             825..1190
                     /locus_tag="OO471_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6245234.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="OO471_004232"
     CDS             1187..1852
                     /locus_tag="OO471_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245235.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="OO471_004233"
     CDS             1852..2217
                     /locus_tag="OO471_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6245236.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          716 a          513 c          684 g          634 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcaga
//
LOCUS       DAJTUM010000236         2542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTUM010000236 DAJTUM010000000
VERSION     DAJTUM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO471_004234"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO471_004234"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6245237.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2542
                     /locus_tag="OO471_004235"
     CDS             1647..>2542
                     /locus_tag="OO471_004235"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6245238.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQEL"
BASE COUNT          662 a          569 c          669 g          642 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ct
//
LOCUS       DAJTUM010000237         2487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTUM010000237 DAJTUM010000000
VERSION     DAJTUM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>2487
                     /locus_tag="OO471_004236"
     CDS             <1..>2487
                     /locus_tag="OO471_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6245239.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAJTUM010000238         2420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTUM010000238 DAJTUM010000000
VERSION     DAJTUM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..201
                     /locus_tag="OO471_004237"
     CDS             <1..201
                     /locus_tag="OO471_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245240.1"
                     /translation="KLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSN
                     SLQLLDETFSVSGTESSATRQH"
     gene            complement(334..846)
                     /locus_tag="OO471_004238"
     CDS             complement(334..846)
                     /locus_tag="OO471_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6245241.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1041..1805
                     /locus_tag="OO471_004239"
     CDS             1041..1805
                     /locus_tag="OO471_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245242.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1756..>2420
                     /locus_tag="OO471_004240"
     CDS             1756..>2420
                     /locus_tag="OO471_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6245243.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          683 a          539 c          614 g          584 t
ORIGIN      
        1 aagctgaaaa tgatgctctt cggcgcaagc ttgataatgg tggtcgggtg ctcgtcaaag
       61 gaaaatgccc tgtgccatcc tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg
      121 ccaccgtcga actctctcca gttgctggac gaaacgttct cggtatccgg gacggaatca
      181 tcagcgacca gacagcattg agaatgcttc aggagtacat caggatccaa tgccttgggg
      241 ggtagcggta attttactca ttatccttca aatcagattc tgttgtcaga ggaatggggg
      301 aagctggatt cgcaagagct ttttatgggc agtttagcta aaaaaaggca acacgaagct
      361 aaaactacca acataaagtg ctgaccagct agcagctgaa atcagactca ctgtatacac
      421 tttcactgga tgaagttgca acattcctgc caccaaagga gcaatgtaac gcagcacggc
      481 gataaagcgc gaagtgaaaa gtattgacac agagttattt tgcaactgaa ggcgaacccg
      541 ttctattgta ttggaacggc tcgatattat acatgcaagt tgagggatat gcccaattat
      601 tataccaaga tggtaattaa cgatagtacc gcaccatgcg ccactcatga ccgtgattcc
      661 agcttcccat ggtgaaagag tcgtcttgct gactgtaatt acagtggcca tcataattga
      721 tgctggtggc aatatcgccg aaatgagtaa agtggatttt gtgaacgcta ttataaaaag
      781 aagcccccat aggcgatatg gatgaagtac aaaatagttc atcaaagcat ttatccactc
      841 catcaatgtc tttccttctg tgaatttttc tttgctattt aaccgtgata cgacttaatt
      901 taggcttaaa caccttacat ttacaaggta ctcctgtggg gggccctgcc acggagcgtc
      961 gcaggagtga gtgatgagga agaattaaag caacctattg tgacaggcag cgataacagt
     1021 aaatcacagg aagcataatc atggcaaaac cggactggga ggccatcgaa tcggcatacc
     1081 gggccggagt ccttagcctc cgtgatatag gcgagaaata cggcgttaca gaaggggcta
     1141 tcaggaagag ggccaaaaag cttgattggg cacgcagtgg cggtacgcag gtttgcaaaa
     1201 atggtacgca aaaaaggaaa gtgcgtacca gcagaaagcc tgccattact ggccttacac
     1261 aaaaaagtac gcaactaaaa acagaatcta caccggatac gaaaccgata cgcggaatgc
     1321 gtaccgatcc cccgactaac ccattccaac ccggtaacca gcaggcatta aaacacggtg
     1381 gttatgcccg tcgccttctg ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc
     1441 tcgaggacga attatttcgc cttcgtgcta acaaccttgt cgctgcagag aatattggtc
     1501 ggtggttggt gtcgctggaa gatgctaatg gggaccagga aaggaagatg ctgattgaaa
     1561 atatcagcgc tgccgagaaa gcaatgatgc gcaatacagt tcgtattgag tccatcgttg
     1621 gcacgcttgc gacggtagga aaaatatttg ctgatacagc ctaccgcaag gccgccactg
     1681 ataaggtgtc tctggaggct gatcgtcttc gccgtgatgc aggtattgat gatggcaatg
     1741 gagagcgtga cctcaatgac ttctactctg acatccaaac cgacgctgaa tccggcttta
     1801 cgtagtttct ggactatgcg ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca
     1861 aaatcatggg atgccgctgg cattgccata tttctgtcga ataaatacac cctgcgtttt
     1921 tgttgtgccc gtcagatcca gaataaaatc gaagagtcgg tgtataccct gctcaaaatt
     1981 cagatagaca ggtttggtct gcggcaccgt ttccgtattc tgaacaacaa aatcattaac
     2041 cgggttactg gctcggaatt tgttttttat ggattatggc gcaacatcga agaaattaag
     2101 tcactggagg ggatcgatat gttgtggctg gaagaagccc acgcactgac ggaataccag
     2161 tggaaaattc tggagccaac gatccgtaaa gagggttcgg aatgctggtt catattcaac
     2221 cccggacttg ttactgattt cgtctggcgc aacttcgttg ttgatccgcc cgaaggcact
     2281 ctcatccgca aaattaacta tgacgaaaat ccgtttctgt ctgacaccat gcttaaggtt
     2341 atcgacgcgg cgcgacgccg tgatccggat ggttttaaac atgtgtatga gggcgttccg
     2401 gagtctgatg atgatgcggc
//
LOCUS       DAJTUM010000239         2327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTUM010000239 DAJTUM010000000
VERSION     DAJTUM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            82..321
                     /gene="relB"
                     /locus_tag="OO471_004241"
     CDS             82..321
                     /gene="relB"
                     /locus_tag="OO471_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6245244.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            321..608
                     /gene="relE"
                     /locus_tag="OO471_004242"
     CDS             321..608
                     /gene="relE"
                     /locus_tag="OO471_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6245245.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            680..835
                     /gene="hokD"
                     /locus_tag="OO471_004243"
     CDS             680..835
                     /gene="hokD"
                     /locus_tag="OO471_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6245246.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1053..1304
                     /locus_tag="OO471_004244"
     CDS             1053..1304
                     /locus_tag="OO471_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245247.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1371..1649
                     /locus_tag="OO471_004245"
                     /pseudo
     CDS             1371..1649
                     /locus_tag="OO471_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1651..>2327
                     /locus_tag="OO471_004246"
     CDS             1651..>2327
                     /locus_tag="OO471_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6245248.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          590 a          555 c          616 g          566 t
ORIGIN      
        1 ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc gatacttgta atgacatttg
       61 taattacaag aggtgtaaga catgggtagc attaacctgc gtattgacga tgaacttaaa
      121 gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc cttctgaagc gcttcgtctc
      181 atgctcgagt atatcgctga caatgaacgc ttgccgttca aacagacact cctgagtgat
      241 gaagatgctg aacttgtgga gatagtgaaa gaacggcttc gtaatcctaa gccagtacgt
      301 gtgacgctgg atgaactctg atggcgtatt ttctggattt tgacgagcgg gcactaaagg
      361 aatggcgaaa gctgggctcg acggtacgtg aacagttgaa aaagaagctg gttgaagtac
      421 ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat gcctgattgt tacaagatta
      481 agctccggtc ttcaggctat cgccttgtat accaggttat agacgagaaa gttgtcgttt
      541 tcgtgatttc tgttgggaaa agagaacgct cggaagtata tagcgaggcg gtcaaacgca
      601 ttctctgaac caaagcatga catctctgtt tcgcaccgaa ggtgacactt ctgctttgcg
      661 ttgacaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat
      721 ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacgaat
      781 ccgaaccggc cagacggagg tcgctgtctt cacagcttac gaacctgagg agtaagagac
      841 ccggcgagtg agaaatccct cgccacctct gatgtggcag gcatcctcaa cgcacccgca
      901 cttaacccgc ttcggcgggt ttttgttttt attttcaacg catttgaagt tctggatggt
      961 gccggaatag aatcaaaaat acttaagtag cgcgcaggga taaaagggat ggaccccgaa
     1021 caggggagtg ctacttatct ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa
     1081 ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa tggaaatatt gttagtcatt
     1141 accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat taattttgtg gatgcccgag
     1201 tgacggacgg atttatcttg cgcgacggtg agtttgtcac ttcattacag gcattgaaag
     1261 agatcggaat aaaagctggc ttttctgctt tttcagaaga ataaactcat ctacaatctt
     1321 gcgcggggct gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca
     1381 acgcaaaata ttacaatcat gataattcga ccgttcttgc ccacacgcac gagcggtatt
     1441 ctcacgcatt taagtcagac tggtaccagc atccctcatg cactgaagaa caggccgaat
     1501 ggcttattca gtgttaccgc aggcgcggat gcgaggttaa aaaagccctt agcctcgact
     1561 accgtcacta gataatctcc gtcaggctcc cttactccga acagccaccg cgtccgtccc
     1621 gcacattcca gcaacggatc tggaggtaac gtgcgggtat tacttcgacc tgttctggtt
     1681 ccggaactcg ggctggtggt ccttaagccc ggtcgtgaat ccatgccggt attccacaat
     1741 acccgggtac tggtggagcc ggaaccgaaa agcatgcgta atctgccgtc cggggtcgtt
     1801 cctgccgttc gccagccgct agtggaagac aaaacattgc tgccgttttt cagtaacgca
     1861 cgggtgattc gtgctgctgg tggtgctggc gcattgtctg actggctgtt gcgccatatt
     1921 aaatcctgcc agtggccaca cggcgattat catcacagcg aaaccgtcat tcaccgttat
     1981 ggtaccggcg caatggtatt gtgctggcac tgcgacaacc agctgcgtga ccagacatcc
     2041 gaatcactcg agcaacttgc tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt
     2101 cacgcaataa gcggtacgca ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg
     2161 gtccgcaatc aggtggcgga cgcgctaccg gaagcggtat tacgtcgttc gctggggttg
     2221 cgtgcggaaa aaatccgctc aatgtaccgt gaaagcgaca tcgtgccggg agagcagacc
     2281 gccaccagca tactgaagca gcgcacaaaa aatcttgcgc cgctgcc
//
LOCUS       DAJTUM010000240         2221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTUM010000240 DAJTUM010000000
VERSION     DAJTUM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO471_004247"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO471_004247"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6245249.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO471_004248"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO471_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6245250.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2221)
                     /gene="arsR"
                     /locus_tag="OO471_004249"
     CDS             complement(1881..>2221)
                     /gene="arsR"
                     /locus_tag="OO471_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6245251.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          590 a          611 c          556 g          464 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 a
//
LOCUS       DAJTUM010000241         2212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTUM010000241 DAJTUM010000000
VERSION     DAJTUM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(495..2099)
                     /locus_tag="OO471_004250"
     CDS             complement(495..2099)
                     /locus_tag="OO471_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6245252.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          564 a          523 c          480 g          645 t
ORIGIN      
        1 ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct tagagttaat
       61 ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct ctaattgtgt
      121 ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt taagttaact
      181 ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt cttattgcta
      241 ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag aacgcttggt
      301 gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta gttgtcctag
      361 aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta tcccttgaca
      421 agtagagaaa actatgagtc aaaaacatga ccacacaggt ctgtagtaag tgtcatccta
      481 gactatggtc tcctctaggt ctccttataa acatcagcca tcacattgac cagcctatca
      541 acctcgacct ctttagcata acggtcgtac gtaatagacc ctgaactatg ccccatgatc
      601 gcctgtgcat acgccaactc gaccccatgt tgttttaact gggccgcaac tgtatgtcta
      661 aacgagtgaa acgctaagcc gctacggtct acgaggactt taggcaacaa cgtccgatta
      721 aaccatttac tcacctgctc accgtaaccg ttcttcatga gacgcagacc atcgaacacc
      781 atagcgtcat caccgtcagc gcctctacgg gtctctacaa gcgccataaa gtcagccaat
      841 ataaaaccat aggccccatc gaccagaggc acacagcggt cactatgagc gttcttgatg
      901 ctcttaccgg gtagactact atcgtcttca ttgatatgaa tataaacggt tcccgcctca
      961 gtggtcctga cgtctttgac ctgtagttgg gctatttcat tgagtctcgc cccagtgata
     1021 gcagccaagg cggtaacata ataatgatac ggcttaccag aggtcttctg agagtatgcc
     1081 ttagctgcga ccagtagttg ccccacctgc tccgtagaga aggcgttacg ggcccctgag
     1141 gctttacgct gagggacttt aagctccaga ccctcagtca tgttcttttt aatcaagtca
     1201 ttgcgcacag cccacctaaa gacagcagcc atcttaataa gatacttgtt gttgatggta
     1261 acgacatcca gacaatctgt cttatcttca cggctcaaca ggtcagcaag tgggacatcc
     1321 ttgaagcgct gcttacggtt cctaggtaac tgctggagca catcacggac cctaagcatg
     1381 tcagccctgt tagcgtctgc attgagaccc agatggtcga atatttcgat taacgcagcg
     1441 tgtgacgcct tgttttccct taaggttgca ggcttccagt tctgatagtt ctccgcttcg
     1501 tattgttcaa agaggctcgc tatggtaatg ctaggcgtaa cttctggttt tacttctgga
     1561 gcattaacag atagggacac agagtctgct tggttatctt gcctatcaat atcattaaaa
     1621 agatcgacgt aatctatcag aggctgactg tctcccgctt cgatacgcct catacaggct
     1681 tttagcacct tcagggcctc attgatgtaa cgatgctggt caatgcttag cggctcacta
     1741 gccagagcat cggtaaggct ctcaccaagt tctccgtatt ggtcccggta gatatccctc
     1801 atgtcaggct caaagagatc tgaacggccc atagacagtt cacactcagc gatgtcctta
     1861 aggtgttccc tcatttcctc ataggtagcg ttgggtctgt ctgcgtgtat tgccttgagt
     1921 gctgccttta tgtgccttga gtaagccata gcggtgcgtc tatccttggt ccgtaatgat
     1981 attgaggtca ttctatcatt gtttgattgt ctgaccctaa gacggaagta gtagacgccg
     2041 ttacgctggt agagatagta gactgctgga agtttgtgac tactggatag ctgaatcata
     2101 tgtctgcatg ccctgagagg gttacacagt gggttacagt aagtaactct gcaaggaggc
     2161 ttaaagccgt atcccttagg attcagtagg ttggagtact ttaagaaagg tc
//
LOCUS       DAJTUM010000242         2189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTUM010000242 DAJTUM010000000
VERSION     DAJTUM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            106..285
                     /locus_tag="OO471_004251"
     CDS             106..285
                     /locus_tag="OO471_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245253.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            337..531
                     /locus_tag="OO471_004252"
     CDS             337..531
                     /locus_tag="OO471_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245254.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1330..1666)
                     /locus_tag="OO471_004253"
                     /pseudo
     CDS             complement(1330..1666)
                     /locus_tag="OO471_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          700 a          447 c          452 g          590 t
ORIGIN      
        1 tattaagact atcacttaaa taagtaatac tggttgtctg gagattcagg gggccagtct
       61 aggtagcgta aggcactaca gcgcattgct ttacgagtaa taacaatgga tttatcacca
      121 gaagacgcaa agtttttacg agaactgcaa caccgtatgc tcagagaagc cattatcgaa
      181 cagcaaacgg gcatgagcgc catagaccca ttgcgataca aacgggcctc atctccttta
      241 gttgacgaca attccaatgc ggataaaaca cgtccaccac actgattaca tttttcatta
      301 gcacagtggt gtgatgcagc atacgaggaa aaccggatga atgagaacaa aataaaaagg
      361 ctggagcaac tgttacaggc acgccaacgg gagtttgcca ctaaatgggg acgtgccgcg
      421 ctgccacatg aacgcctaac ctattttcca cttgaagacc tcattaaaaa taatggcaaa
      481 aaaatcaggt ctgataagaa acacttctta gaatgcaatg aagacaaata acaacaatct
      541 gcatattgcc accaaccaca gatgtggcat cgttatcagc gcacttgatt ttctttatat
      601 tcactgacga gcttgccggg gcacctttgt ttgccggtca ttctggtatc atgcagatat
      661 ttacggcgta caagcccact agcatgacat tgtaataagg cctgagccta acgctttaat
      721 gtgtgtcata cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc
      781 cagcgaaatc catctggtca taatctgaag catccgacaa agtggtgtcc accaaataag
      841 tgatctgcgc ccaatatcct gagccggaac tttgacaaag gccaacgaaa aatgggagcg
      901 gatgttacag agttctcggt acaggggaaa agtggtacct gtcgccagtt ctccgatctg
      961 tttaaccggg aaattatctc ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa
     1021 tacgatgcta tacgatgcat tcacagtgcc cggccagagg atgccccgtc gctgcatatg
     1081 gatcagggtt ggcaatatcg aatcgcaggc tataggcaaa gttaaagccc catggagtag
     1141 cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag aacttcttca
     1201 gcacactgct aaaacgcata gtgatcgagc gctgattctg gcgaacaact gaactaatac
     1261 atcagaatct gcattatgtt aaataaatat gaaaagatgg tttaaatacc ccgttacttt
     1321 tgacttacac tatacggtat cgcatcgttt aatattcgca ccggccagat ttttatttct
     1381 attagttgtc acaatactga atgcgtacga ccacagtatt ctggctcctg tgtggttatg
     1441 ctttaattct gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat
     1501 gtcagttccc ctttttacca gtgctgattt ccacattccg tccaacagag cttatagcct
     1561 ttccctggat tatagcattg tccggctgaa gttctttttg aataataata gaagcactgc
     1621 tggcagatcc agtccgtttt tcataaccca ctgtactgat aaccataatc taatcagtag
     1681 aaattgagtc gaaaataagc actactctat acaggataat tagaggtcag tttgattatt
     1741 cacaattcat catcagcatt ttctatttct gacgaaatca acatgaaaat aaccatatat
     1801 gataattatt ataataacgg ctttaattgg aatacatata ttacaacgta ttatatataa
     1861 ttggtattct gggaactata ttctcaaaat acagtagaaa cgaggtatgt ttctggtgga
     1921 aaggacagtg ggattaaaaa gtaagaattg ataaaaaaaa cgccagcaac acatagtgcc
     1981 ggcggaggga ataccccatg gagaaaatgt gatgcctaga gcatcatgaa tatataatta
     2041 aaaatagtta gcgttgtcaa tactttcaca aaaataattt tcgtagtata gaaagatatt
     2101 tttatgcatg acctacctga atttgctccg ggtagaggtt ataaataaaa attgaatcac
     2161 gacaaacaca atattcatag tatggcgat
//
LOCUS       DAJTUM010000243         2177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTUM010000243 DAJTUM010000000
VERSION     DAJTUM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(220..384)
                     /locus_tag="OO471_004254"
     CDS             complement(220..384)
                     /locus_tag="OO471_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245255.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OO471_004255"
     CDS             1286..1570
                     /locus_tag="OO471_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6245256.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OO471_004256"
     CDS             1558..2043
                     /locus_tag="OO471_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6245257.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          562 a          457 c          620 g          538 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatg
//
LOCUS       DAJTUM010000244         2143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTUM010000244 DAJTUM010000000
VERSION     DAJTUM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO471_004257"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO471_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6245258.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="OO471_004258"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="OO471_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1984)
                     /locus_tag="OO471_004259"
                     /pseudo
     CDS             complement(1294..1984)
                     /locus_tag="OO471_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          626 a          396 c          491 g          630 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     1501 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     1561 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc
     1621 cagtgttgtt gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg
     1681 gcaacatcag cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg
     1741 tccgtatgac ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc
     1801 cgttatgtgc catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt
     1861 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
     1921 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
     1981 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
     2041 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
     2101 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aat
//
LOCUS       DAJTUM010000245         2113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTUM010000245 DAJTUM010000000
VERSION     DAJTUM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..114
                     /locus_tag="OO471_004260"
     CDS             <1..114
                     /locus_tag="OO471_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6245259.1"
                     /translation="RLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(263..1717)
                     /gene="ospC2"
                     /locus_tag="OO471_004261"
     CDS             complement(263..1717)
                     /gene="ospC2"
                     /locus_tag="OO471_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6245260.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          661 a          372 c          350 g          730 t
ORIGIN      
        1 cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag aaattacctg
       61 gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa ttaagggaca
      121 cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt tgcaggggaa
      181 cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc agattcaggg
      241 cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc gctccatttt
      301 ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca agcatagtgt
      361 ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta ctaataaaat
      421 attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca tcatacagct
      481 cacaatacat attgttgttc attttctcca tctctgcgat atcttgtttt gtaaaattaa
      541 atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa taagcaagag
      601 cttgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa ttggatgctt
      661 taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta tctgtgctta
      721 gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta tccagactaa
      781 caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta ctctttctta
      841 taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtatta aatattggca
      901 catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca acctctttaa
      961 agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata gcattatcaa
     1021 agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata tacgcattag
     1081 caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga tgattggaaa
     1141 tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc tcagagaatg
     1201 tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta atattaagag
     1261 tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa ttaataataa
     1321 tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt atttcagaaa
     1381 gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac cgtgctccag
     1441 atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag aacattttac
     1501 tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca gcacagtgat
     1561 ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat atgttttttt
     1621 gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca atattgttct
     1681 gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca ttatgtgcgt
     1741 ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg tatgtattat
     1801 aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga gtttaatcac
     1861 acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat atatatcttg
     1921 cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg caaaacaggg
     1981 catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa gaatcatggg
     2041 cgctcaacaa aaagagggta atgctgccaa cttactgatt tagtgtatga tggtgatttt
     2101 aaggtgcttg cgt
//
LOCUS       DAJTUM010000246         2093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTUM010000246 DAJTUM010000000
VERSION     DAJTUM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..800
                     /locus_tag="OO471_004262"
                     /pseudo
     CDS             1..800
                     /locus_tag="OO471_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            915..1256
                     /locus_tag="OO471_004263"
                     /pseudo
     CDS             915..1256
                     /locus_tag="OO471_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1239..2076
                     /locus_tag="OO471_004264"
                     /pseudo
     CDS             1239..2076
                     /locus_tag="OO471_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          439 t
ORIGIN      
        1 ttctgggcct tgcccgtagc agttattttt atcaccgggc tgcactgaaa gccggtgata
       61 aatacgccac gatacgtaag atgctgacag atatctttaa cagtaattac cagtgttatg
      121 gctatcgtcg cctgcatgcg atgctcaggc atgagggggg gcggctatca gaaaaggttg
      181 tacgcagact tatggtggag gaacagcttg tcgtcagtcg taaccgtcgt cgtcgctaca
      241 gctcatattg cggagaaatc ggaccggctc cggataacct tatcgccaga gattttaagg
      301 cggagcaacc taatcagaaa tagctgacag atatcacgga gttccagctc cctgcaggta
      361 aagtctggct gtcaccagtg gtggactgct tcgatggaaa agttgtgagc tggtctctca
      421 gtacacgccc tgatgctgaa ctggtcaaca ctatgctgga tagcgctgtc gaaacgttaa
      481 atgctggcga acgaccggtg atacacagtg acagaggtgg gcattatcgc tggccaggct
      541 ggctggaaag agtgaatgca gcaggtctta ttcgctcaat gtcccgtaaa ggatgttcac
      601 ctgataatgc tgcatgcgaa ggctttttcg gcagactgaa aacggaaatg tattatgggc
      661 gtaaatggtc gggcatcacg ccagaaaagt tcatgcagca agtggatgct tacatcagat
      721 ggtataacga gcggcgtata aaattatcgc tgggtgccgt cagccccaaa atgtaccgcc
      781 aacaatgcgg gctggaatga taaagcagtc caggaaatcg tccgcatccc cccttcacca
      841 tttggattgt ccgctccacc caacatgttg tttccttaag gttcctacac cacaaaggaa
      901 actctcatgc tgcgcagtgg atggctgacg ttgccgacag gcgggaactt cgccagttca
      961 ggcagacacc ggaacagcgc ttcacgcagg aacaggagca tctgcagccg ctgctgggta
     1021 cggacttcga tatccgtcat gtctcctggg atggctatat cgaggttggc gggaaccgtt
     1081 acagtgtgcc ggaaagcctg tgtggtcagc tggtatcaat acggatctcg ctggatgatg
     1141 aactgcggat ctacagtaat gagcaacagg tagcatcgca ccgtctctgt tcggctgcat
     1201 acggctggca gaccgtgccg gagcatcacg ctccactctg gcagcaggct tcggagcgca
     1261 tggccgaatg gctgggggag atacagaaac gggtaatcac tgtctgtgac cgggaagcgg
     1321 atatctggca ttacctgtat tataaagtca gccacggaca gcgtggtgcg tgctgcacag
     1381 aatcgccggc tggaagaggc acccggcaag ctcttcgaac tgccggaagt cctggcaacc
     1441 gcaggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg tcaggcccgg
     1501 atgttcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg ccaggcgctc
     1561 ccgctcacgt atgtctgttg ccgggagcag gcagaggacg gtgcctgctg gcatctgctg
     1621 acgtcaggaa aagcggcgag tgccgccgat gcacgacgta ttgtcagcca ttacgagcga
     1681 cgctggctga cagaggaata ccacaaggcg tggaaaagtg gtggtacatg gaatcgctgc
     1741 gaatgcagac ccgggataac cttgagcgca tggtggttat cctggcgttt atagcggtac
     1801 gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac acaaaatgac agctgtgaga
     1861 aaatactgac gccgacagag tggaaactac tgtgggttaa gctggaagga aagccgcttc
     1921 cggtgcaggc accgaccctg aaatgggcct ggggcgatgg catgacagca aacgcacagg
     1981 tcgtcccggt tggggcgtca tgtgggatgg ctggtcagac ttcaggatat ggttgagggt
     2041 tacctggtga tgaagtctct tgatcaggag ctctgatcaa gagacagcag ctt
//
LOCUS       DAJTUM010000247         2082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTUM010000247 DAJTUM010000000
VERSION     DAJTUM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            106..1143
                     /gene="curA"
                     /locus_tag="OO471_004265"
     CDS             106..1143
                     /gene="curA"
                     /locus_tag="OO471_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6245261.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1341..2006
                     /gene="mcbR"
                     /locus_tag="OO471_004266"
     CDS             1341..2006
                     /gene="mcbR"
                     /locus_tag="OO471_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6245262.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          575 a          441 c          557 g          509 t
ORIGIN      
        1 tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca atcccgtgag
       61 cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg gcaacaaaag
      121 cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt tccggagaat
      181 ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt actgcgcaca
      241 gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc atcttattca
      301 ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt cgtggagtcg
      361 aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg gcaggactat
      421 gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa tccatcgtgg
      481 tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct ggatatcggt
      541 cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt gggggcgacg
      601 gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg tggcgcggaa
      661 aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca ccacgcggac
      721 gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta ttatgaaaac
      781 gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc gcgcattccc
      841 gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc ggatcgttta
      901 cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt tattatcgct
      961 caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg ggtgaaagag
     1021 gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc acagacgttt
     1081 atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc gggtgatgat
     1141 taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct tattttcagc
     1201 tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg ggtggtttat
     1261 gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg gttggacaga
     1321 aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc agtttgactc
     1381 tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa cctggcgcac
     1441 gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct gtgcgtgaag
     1501 cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa gcatttacag
     1561 taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac gaactggaat
     1621 taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca gaacttcagg
     1681 aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa atcattaatg
     1741 tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc ctgtgtgaga
     1801 tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat gaagcgatta
     1861 acccagcgga attacgggag cgcatagaaa actatcatct attactcgct gcgttaaaag
     1921 caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa aatattgcta
     1981 ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg catcaagtaa
     2041 ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAJTUM010000248         2076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTUM010000248 DAJTUM010000000
VERSION     DAJTUM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..694
                     /locus_tag="OO471_004267"
                     /pseudo
     CDS             1..694
                     /locus_tag="OO471_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            696..1301
                     /locus_tag="OO471_004268"
     CDS             696..1301
                     /locus_tag="OO471_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6245263.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2076
                     /locus_tag="OO471_004269"
     CDS             1288..>2076
                     /locus_tag="OO471_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245264.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGV"
BASE COUNT          502 a          491 c          579 g          504 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgtt
//
LOCUS       DAJTUM010000249         2034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTUM010000249 DAJTUM010000000
VERSION     DAJTUM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..364)
                     /locus_tag="OO471_004270"
     CDS             complement(<1..364)
                     /locus_tag="OO471_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6245265.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..1645)
                     /locus_tag="OO471_004271"
     CDS             complement(431..1645)
                     /locus_tag="OO471_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6245266.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          494 c          413 g          494 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtacgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      961 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
     1021 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
     1081 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
     1141 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
     1201 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
     1261 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
     1321 gcagttgata caatggcata tttgccgctt tagctttgat atcccacaac gccatatcaa
     1381 tggcagaaat ggctgacatc gtgacggggc cacgtcgcca gtaagcacct ttataaaaga
     1441 attgccagat atcttcgata cggctggcat cgcggccaat tagttgtgga caaagatgat
     1501 ctttcagata agaagcgacc gacaattcgc gaccatttaa cgtggcatct cctaatccac
     1561 aaagtccgct ttcagtggtt attttaagcg taacgaaatt acgccctggg caagtaacaa
     1621 aaacatctgc agcaataatt ttcattattg caatctcctg tgttgtaaat agacgtttca
     1681 aacctaattt tacgcctact accatacaag agagaaaaag cgattattca accgtgatca
     1741 cattaaaaac aaaaatttct accgaggaga aaaaataccc tcaaaaacca cctgaataat
     1801 taaccattat tgttaaaaca acacgttgaa taatcacata acaaactcca ttaatcattg
     1861 aaaatgagca cagaaataga aattaattat attcaattaa ttaaaatgtg tattgcttga
     1921 tgtatttaaa aatattattt tcatggtgag agtggaggta aagaagacac aataataaaa
     1981 aggaatgtga caaataatgg ttctggttgg gcgttaaatt taaggtcacc agaa
//
LOCUS       DAJTUM010000250         2034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTUM010000250 DAJTUM010000000
VERSION     DAJTUM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..2034
                     /gene="sat"
                     /locus_tag="OO471_004272"
                     /pseudo
     CDS             1..2034
                     /gene="sat"
                     /locus_tag="OO471_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          747 a          356 c          400 g          531 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc agat
//
LOCUS       DAJTUM010000251         1969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTUM010000251 DAJTUM010000000
VERSION     DAJTUM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            240..509
                     /locus_tag="OO471_004273"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO471_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO471_004274"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO471_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO471_004275"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO471_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6245267.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1690..1969
                     /locus_tag="OO471_004276"
                     /pseudo
     CDS             1690..1969
                     /locus_tag="OO471_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAJTUM010000252         1963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTUM010000252 DAJTUM010000000
VERSION     DAJTUM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..354
                     /locus_tag="OO471_004277"
     CDS             <1..354
                     /locus_tag="OO471_004277"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6245268.1"
                     /translation="RKGCPNYPPEFKQLLVAASCEPGISISKLALENGINANLLFKWR
                     QQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGN
                     VSEKLLTLLIQELKR"
     gene            351..698
                     /gene="tnpB"
                     /locus_tag="OO471_004278"
     CDS             351..698
                     /gene="tnpB"
                     /locus_tag="OO471_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6245269.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            748..>1963
                     /locus_tag="OO471_004279"
     CDS             748..>1963
                     /locus_tag="OO471_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HCR6245270.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGS"
BASE COUNT          444 a          555 c          568 g          396 t
ORIGIN      
        1 cgaaaaggct gccctaatta tcctcccgaa tttaaacagc tgctcgttgc tgcctcctgt
       61 gaacccggga tatccatctc aaaacttgct cttgaaaatg gcattaacgc caatctgttg
      121 ttcaaatggc gacaacaatg gcgcgaggga aagctgctat taccttcttc agagagcccc
      181 cagctacttc ctgtgactct cgatgcagct gccgaacagc cagaatcgct cgcagaggac
      241 ccggaaaccc tcagtatcag ctgtgaggta acgttccggc acgggacgct ccgcttcaat
      301 ggcaatgtca gcgaaaagct cctgactctg ctgatacagg aactgaagcg atgatcccgt
      361 taccttccgg gaccaaaatt tggctggttg ccggtatcac cgatatgaga aatagcttca
      421 acggcctggc tgcgaaagta caaacggcgc tgaaagacga tcccatgtcc ggccatattt
      481 tcattttccg gggccgcagc ggcagtcagg ttaaactgct gtggtccacc ggcgacggac
      541 tgtgcctcct gaccaaacgg ctggagcgtg ggcgcttcgc ctggccgtca gcccgtgatg
      601 gcaaagtgtt ccttacgcag gcgcagctgg cgatgctgct ggaaggtatc gactggcgac
      661 agcccaagcg gctgctgacc tccctgacca tgctgtaaat ctctttatcc tggttgtcac
      721 agaataagcc cggtaaaata cgggcttatg aacgacatct cttctgacga catcttcctg
      781 ctgaaacagc gcctggccga acaggaagcg ctgatccacg ccctgcagga aaagctgagc
      841 aaccgggagc gcgaaataga ccatctgcag gcgcagctgg ataaactccg ccggatgaac
      901 ttcggcagtc gttccgaaaa agtctcccgc cgtatcgcac aaatggaagc cgatctgaac
      961 cggcttcaga aagagagcga tacgctgact ggtagggtgt atgacccggc agtacagcgt
     1021 ccgttgcgtc agacccgcac ccgtaagccg ttccctgaat cactaccccg tgacgaaaag
     1081 cgactgttgc ctgcggcgcc gtgctgcccg aactgcggcg gttcactgag ctatctgggc
     1141 gaggataccg ccgaacagct ggagttgatg cgtagcgcct tccgggttat ccggacggta
     1201 cgggaaaaac atgcctgtac tcagtgcgat accatcgtgc aggcacctgc accttcgcgg
     1261 cccatcgagc ggggtatcgc cggaccgggg ctgctggccc gcgtgctgac ctcgaagtat
     1321 gcagagcaca ccccgctgta ttgccagtca gaaatatacg gccggcaagg tgtggagctg
     1381 agccgttcac tgctgtcggg ctgggtggat gcatgctgcc ggctgctgtc tccgctggaa
     1441 gaggcgcttc atggctatgt catgactgac ggcaaactcc atgccgatga taccccggtc
     1501 caggtactgc tgccgggtaa taagaagacg aagaccgggc ggttgtgggc gtatgttcgt
     1561 gatgaccgca atgccgggtc agcgttggca cctgcagtgt ggttcgctta cagcccggac
     1621 agaaaaagca tccatccgca gactcatctt gcttgcttca gcggtgtgct gcaagcggat
     1681 gcgtacgccg ggttcaacga gctgtatcgc aatggtggga taacggaagc tgcctgctgg
     1741 gctcatgccc gccgaaagat ccacgatgtg cacgtccgca tcccgtcagc actgacggaa
     1801 gaagccctgg agcagatcgg tcagttgtac gccatagagg cggatataag gggaatgccg
     1861 gcagagcagc ggcttgctga acgtcagcga aaaacgaaac cgctgttgaa atccctggaa
     1921 agctggttgc gtgaaaagat gaagaccctg ttcttcggct ctg
//
LOCUS       DAJTUM010000253         1910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTUM010000253 DAJTUM010000000
VERSION     DAJTUM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            353..1711
                     /locus_tag="OO471_004280"
     CDS             353..1711
                     /locus_tag="OO471_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR6245271.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          505 a          473 c          514 g          418 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa
//
LOCUS       DAJTUM010000254         1905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTUM010000254 DAJTUM010000000
VERSION     DAJTUM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO471_004281"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO471_004281"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6245272.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO471_004282"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO471_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6245273.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..>1905
                     /locus_tag="OO471_004283"
     CDS             1599..>1905
                     /locus_tag="OO471_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6245274.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     "
BASE COUNT          443 a          525 c          492 g          445 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtg
//
LOCUS       DAJTUM010000255         1903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTUM010000255 DAJTUM010000000
VERSION     DAJTUM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(53..1702)
                     /locus_tag="OO471_004284"
     CDS             complement(53..1702)
                     /locus_tag="OO471_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6245275.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          450 a          502 c          489 g          462 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctc
//
LOCUS       DAJTUM010000256         1898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTUM010000256 DAJTUM010000000
VERSION     DAJTUM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..1824)
                     /locus_tag="OO471_004285"
     CDS             complement(<1..1824)
                     /locus_tag="OO471_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6245276.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
BASE COUNT          459 a          555 c          395 g          489 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagc
//
LOCUS       DAJTUM010000257         1888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTUM010000257 DAJTUM010000000
VERSION     DAJTUM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..682)
                     /locus_tag="OO471_004286"
     CDS             complement(<1..682)
                     /locus_tag="OO471_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245277.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRE"
     gene            complement(687..1385)
                     /locus_tag="OO471_004287"
     CDS             complement(687..1385)
                     /locus_tag="OO471_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6245278.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(1385..1714)
                     /locus_tag="OO471_004288"
     CDS             complement(1385..1714)
                     /locus_tag="OO471_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245279.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            complement(1714..>1888)
                     /locus_tag="OO471_004289"
     CDS             complement(1714..>1888)
                     /locus_tag="OO471_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6245280.1"
                     /translation="GMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQ
                     QARDGGRLSGAYR"
BASE COUNT          405 a          627 c          446 g          410 t
ORIGIN      
        1 attcccggtg acgccagagg gagtgtgtgc gtcgctgcca tttgtcggta tacctctctc
       61 gtttactcag ttgttcagga atatggtgca gcagctcacc atcgccgcag taaatggcgg
      121 catgattcgg caccgatgaa ccaaaacagc acagcagcac atcgcccggt tgtgctgatg
      181 acaacggcac ctgatacagc cctgtggcct ccagattatc cagatagaga ttctgaccgt
      241 gacgccacca gtcatccccg cgatgaaaat ccggcatctc aatccccgcc agatgataag
      301 catcccggaa cagcgtgtaa cagtccgtca ccccgtgctc aaagcgccgc ccggtgagat
      361 gcggcacaca gcggaacttg tgaatcgtcc cccggcagac cagccaccac ggcaaatcac
      421 tctgcacctg cagccgccgg tcagcctcac tcagccaggg cagaccaccg ggatgactgt
      481 ggaccagtgc cacaatctca ccctgcattt gtgcccgcag ccagtcctcc ggagccatcc
      541 ggaaccgttc ctccggctca ccggagatat tctcgctggg aagatacctg tctccctccg
      601 gcgttctcac cacgaagcca cacgactccg ctggcgcaca tcgccgggcg tgcgccagaa
      661 tcgctgattc tgtctgtgtc atggatttac tgagaaagtt tattgatgga aaggaaaccg
      721 ccgaaattac cggtattgtt gcgcaactca cacccgcgca tacacttgct gcatttgtcc
      781 ttccggatat ccgttgttgg tttgtcgaac tcgtcagcca ctgccctgcc cgtgtaacca
      841 cactcatcag aacggtaagt ccacatacag gtattcgcca gcatgatacg ccccgggaaa
      901 accgcgccat ccgtctccgt tggtgtggcc agcacaaaag aggcactgac cgcgctcagt
      961 tcgctgcact gctcgatgcg ccagcggctg atcacctcct gttccggatc ggcgtcgctg
     1021 tttccgttga cgaagttcac cgcatccaga aaacgggcgt aaaccttacg ccggaccacc
     1081 gttccgccga ccagactctg cagatcttcc gccatcccgg tgaccatgcc gtgcaggtta
     1141 gagaccgtca gtgtcggacg ggcagcacag cccctgccgt tcagttcaaa ccccgacccc
     1201 tgaatggggt atgcctgata ctgccgcccc tgccaggtga ccggctcacc tttttcgttc
     1261 tgctcattac agaaaaaata acgctcacca ccgacctctg tcagatcgat ttcccagagc
     1321 tccacccggg ctgactgctc cgcacgggtg cattcattca gtgtttcctg ccggatatcc
     1381 tgcatcagtt caccacctgt tcaaaatcgg ccgttacagt cacccataac gcccccacgc
     1441 ttgccgacca tttacgacaa accaccctga tcagtttcca gtcataaggt ggcgtccact
     1501 gaaatgcgcg gacgccaccg tgccgttcca gaaaggcttt taaagatggg tgttcacctt
     1561 tacgaacacg tatcgtcacg ctgtaagtcg acaactggtt attcagtccc gccgcacgac
     1621 gctgttcata accatcgccc agcttcactg tcaccacttt cggctctgat accacattca
     1681 tatccgggcg cactttccag tgaaacgtct ccattaccgg tatgctccac ttaaccggcc
     1741 accatcacgg gcctgctgtt gcataaagtc cgcggccgct tttttcccga ggtcataaac
     1801 caccttcagg gcagccggac ctatctgccc gttcgtgccg tcgttattga tctcaatgtt
     1861 gtactgcggg gcaaacatca ccatgcct
//
LOCUS       DAJTUM010000258         1878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTUM010000258 DAJTUM010000000
VERSION     DAJTUM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            375..518
                     /locus_tag="OO471_004290"
                     /pseudo
     CDS             375..518
                     /locus_tag="OO471_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            665..931
                     /gene="ospE1"
                     /locus_tag="OO471_004291"
     CDS             665..931
                     /gene="ospE1"
                     /locus_tag="OO471_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6245281.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1322..>1878
                     /locus_tag="OO471_004292"
     CDS             1322..>1878
                     /locus_tag="OO471_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6245282.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          633 a          393 c          314 g          538 t
ORIGIN      
        1 cttcttacca tatttgaaat aaagctcaat agcgcgaatt ttttgttctt cagtaaacat
       61 gtttcatctt ctgagagtcc aggaaatcgt ccgcatcccc attaccgtct tatatcactg
      121 gcgctgacat gtgctcattg atgaagtgaa tctgaatgac atgttttgcc atatcaacgc
      181 caaccggtgt atatttcatt tgtggagtct ccagtctgag gacgctttat gcatcccgta
      241 ttgggcagta tgatgccgga aagctgcgag gctccacacc tgccagtttc acccttttaa
      301 ccatcatact actccgtgtt taattgtgtg ggatgcgttc attacatttt aacagtgtca
      361 ccccgtcggc cacgaaagat aaacaggtga ccagaaaagg gatcttcatc cagcacatgc
      421 tatatcttga acgtaacaac ctgaccctcc gcacccgcat caagcggctg gcacgtaaaa
      481 caatctgctt ctcgcgttcc gtggaaatcc acgaaaagtc atcggctcct tcattgaaaa
      541 acacatattc tactgattgg aagcgccacc cgaaaaagta tcgttttttt acagtaaact
      601 tcatttagcc gactataatg taaaaaaata aattttttac caaaatataa cataaggaag
      661 taatatgctt acacaaacta tattcccttg tcttccacaa aaacaagaaa atatcatcct
      721 tgaagtatca aatcctgtat tattatcttc tactgttact acagatggat atactgtttt
      781 caataaaaaa gcagctatat acgaattaca aatacctgct gccagccgga cgaaaacgtt
      841 gaaattcact gctacagaaa tgcaatggtt gacaaagata aatgaagccg gaattgatga
      901 aaaacaaagc caacggtatt ctgatttctg aagttaattt cagactcgtt taagctccat
      961 tttcaaaaaa aatatatcag ggtttgttac taataacttc tgaaaacaga aaaaaaatag
     1021 agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt
     1081 atcgtgatat attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg
     1141 accttaaata cctttttatg gctgaattac cattctcagg aataacagca gactccttcc
     1201 catagacggc ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa
     1261 aattaataag ccatattcta tttaactaga attcttatcg ttgataagaa ttctagttat
     1321 aatgattaaa tcaaccaata tacaggcaat cggttctggc attatgcatc aaataaacaa
     1381 tgtatactcg ttaactccat tatctttacc tatggaactg actccatctt gtaatgaatt
     1441 ttatttaaaa acctggagcg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat
     1501 cgccttcaat aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga
     1561 gttagattta aaaacattac cagatttacc gcctcagata acaacactgg aaataagaaa
     1621 aaacctatta acacatctcc ctgatttacc accaatgctt aaggtaatac atgctcaatt
     1681 taatcaactg gaaagcttac ctgccttacc cgagacgtta gaagagctta atgcgggtga
     1741 taacaagata aaagaattac catttcttcc tgaaaatcta actcatttac gggttcataa
     1801 taaccgattg catattctgc cactattgcc accggaacta aaattactgg tagtttctgg
     1861 aaacagatta gacagcat
//
LOCUS       DAJTUM010000259         1836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTUM010000259 DAJTUM010000000
VERSION     DAJTUM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..344)
                     /locus_tag="OO471_004293"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="OO471_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="OO471_004294"
     CDS             complement(344..1264)
                     /locus_tag="OO471_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6245283.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1836
                     /gene="pgrR"
                     /locus_tag="OO471_004295"
     CDS             1402..>1836
                     /gene="pgrR"
                     /locus_tag="OO471_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6245284.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          459 a          447 c          424 g          506 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtc
//
LOCUS       DAJTUM010000260         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTUM010000260 DAJTUM010000000
VERSION     DAJTUM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(208..609)
                     /locus_tag="OO471_004296"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO471_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO471_004297"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO471_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6245285.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTUM010000261         1809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTUM010000261 DAJTUM010000000
VERSION     DAJTUM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            173..1198
                     /locus_tag="OO471_004298"
     CDS             173..1198
                     /locus_tag="OO471_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6245286.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="OO471_004299"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="OO471_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAJTUM010000262         1709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTUM010000262 DAJTUM010000000
VERSION     DAJTUM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(110..577)
                     /locus_tag="OO471_004300"
     CDS             complement(110..577)
                     /locus_tag="OO471_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6245287.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO471_004301"
     CDS             complement(643..882)
                     /locus_tag="OO471_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6245288.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO471_004302"
     CDS             complement(1017..1622)
                     /locus_tag="OO471_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6245289.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          546 a          377 c          303 g          483 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaa
//
LOCUS       DAJTUM010000263         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTUM010000263 DAJTUM010000000
VERSION     DAJTUM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..360
                     /locus_tag="OO471_004303"
     CDS             <1..360
                     /locus_tag="OO471_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6245290.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO471_004304"
     CDS             361..726
                     /locus_tag="OO471_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6245291.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO471_004305"
     CDS             723..1406
                     /locus_tag="OO471_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6245292.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWVWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          452 a          323 c          438 g          387 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggtgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJTUM010000264         1591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTUM010000264 DAJTUM010000000
VERSION     DAJTUM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(333..479)
                     /locus_tag="OO471_004306"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="OO471_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="OO471_004307"
     CDS             533..715
                     /locus_tag="OO471_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR6245293.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="OO471_004308"
     CDS             complement(816..1145)
                     /locus_tag="OO471_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6245294.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1591)
                     /locus_tag="OO471_004309"
     CDS             complement(1317..>1591)
                     /locus_tag="OO471_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245295.1"
                     /translation="LNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLNDAVE
                     ELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          430 a          366 c          375 g          420 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat a
//
LOCUS       DAJTUM010000265         1481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTUM010000265 DAJTUM010000000
VERSION     DAJTUM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..586)
                     /locus_tag="OO471_004310"
                     /pseudo
     CDS             complement(1..586)
                     /locus_tag="OO471_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(634..1206)
                     /locus_tag="OO471_004311"
     CDS             complement(634..1206)
                     /locus_tag="OO471_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6245296.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          440 a          312 c          289 g          440 t
ORIGIN      
        1 caacgatgac atccatcaca ctgatatgaa ttgggttggg attctctgct atcagatttc
       61 gcccttcccg gcgccatttc actttcggcg cagcatcttc gggaataagg cggatatttg
      121 ctggtcgcca gaacagcttg atacgcgttt ttactgctat ttccagcgag ttcgtcgctt
      181 tgttgctcgc tgatggcgga atatttttaa tattcagcca gaagactgac tcgcgatcat
      241 caggcaatga cacacctttg gtgtgaataa tgcgtaatat tttttcttgc gaaggttcta
      301 atcgtgatac tggcggaata acggtaaacg gcgctttgct tttatcctct ttattaaatg
      361 gatctaccca agcctggatg agataaggta ttttttcttc tttgctaaat acagaaatag
      421 atttatcagc attattactt aagtaaacca cccgcgtact accaatgaca acgcctgcat
      481 aagttaatgc tggttggcct aaaaaaacgc tgagtataat cgcggtaaat aacttacatt
      541 aaactgttgt tttaaagagc ctgattaaac aactttttct ggacataacg actccctggc
      601 caaagccaac tgaataaaaa ggcccttgat aaattatttc ttatattcga cagtaaattg
      661 tgaatcagcg ttcgccgtac cagccgttac ggtcgcattg gttgccacat agcgtgcaat
      721 aaacttcatt tcagccttgc cttcagcatc gatagcggta taagcagagt cgttataaag
      781 tttaacctgc gaattatcgg actggttaaa aatacgaata ccaacgccgg ttgccgcaat
      841 cgcttgtcct gtaccggtaa aatcaccagg gttgcctgct gtaagcggcg tacccacttt
      901 caggtcgccg tcgccgccag gcgcttctgt accatcaaaa cgaacggctg cttgagtgta
      961 cgttgccggg caattttcaa gtttaatact gaacttctgt ggcgacgcca gactacctac
     1021 acctgaaaat gcatttttac tgactttacc cagtgggaca atttgatcgg ctgtatcgga
     1081 ggtaacttca caagtagtgt cgacaatttc accgacgaag ttaacaacac catcttctgc
     1141 cagtgcatgc ccggccagca ttagagatgc cagagtgaat gcaccgccta taatattacg
     1201 cttcataaaa actccattga tataatatat cctttaccat gaatcatcac ttatattaat
     1261 aagtgtgttt ttgattcaaa ggaatagtac accaaagaga cggctttatc taaaaataat
     1321 attaattacg caaaaataaa atatagattt attgtttaaa tattatttaa acagtaaaaa
     1381 atcaagtttg ttatcagaca ttaatgataa ttcagaatat atttatatag aaagatagat
     1441 gaatttattg atgtcagtta taaaaaaggg ccaccggtcg g
//
LOCUS       DAJTUM010000266         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTUM010000266 DAJTUM010000000
VERSION     DAJTUM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..167
                     /locus_tag="OO471_004312"
     CDS             <1..167
                     /locus_tag="OO471_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6245297.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="OO471_004313"
     CDS             152..790
                     /locus_tag="OO471_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6245298.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="OO471_004314"
     CDS             1018..>1404
                     /locus_tag="OO471_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245299.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAJTUM010000267         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTUM010000267 DAJTUM010000000
VERSION     DAJTUM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            400..1149
                     /locus_tag="OO471_004315"
     CDS             400..1149
                     /locus_tag="OO471_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6245300.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          388 a          278 c          274 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTUM010000268         1365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTUM010000268 DAJTUM010000000
VERSION     DAJTUM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..682)
                     /locus_tag="OO471_004316"
     CDS             complement(<1..682)
                     /locus_tag="OO471_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6245301.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFD"
     gene            complement(1281..>1365)
                     /locus_tag="OO471_004317"
     CDS             complement(1281..>1365)
                     /locus_tag="OO471_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6245302.1"
                     /translation="ADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          361 a          219 c          332 g          453 t
ORIGIN      
        1 tgtcaaaata accgttcctt aaattttccg gcaggtatgg aatatcggtt aacatattgt
       61 cgattgccca tatggagtga atgttgtgag gtagaggggg gagttcacta agaagattcc
      121 ctattgcgct aatctcttgt aaagaaaatg gtaaaggtgg taaaactgcc agcccattac
      181 atgatacatc taatgtttcc agtagttctg gtaaaacagg aagagaacat aattgattat
      241 ttgagacatg aagctccttc aaagatatgg ggagtgtggg tagtgtgatt agttgattgt
      301 gggacgcatt caataattta agtccttgag gcaaagcagg cagttcaata agtctgttat
      361 agctgacatt aagttgtgta agggacgcag gcaatgggga gattaagctt aaattatttt
      421 tacttatatt aattgattta attcccgggg ggatttcagg taatgttgtc aggcctaatt
      481 cagacaagtc caggttcgtc tcttggtttt gtagacatga tactagtcgc tgaaaagcga
      541 tgtctcgttg ttcttcttgt atgcggttat ttttccattc agtccaatgg gttagataat
      601 tttcatatgc gcggctagtg tcgattgaat aagtggagaa cgaatttgaa attaatctgt
      661 gattgttatt tgtcgggagc ataaatatca gggtgtattc tttatgttga gaggtgttgg
      721 attctttttt atctgctttg caatgttatt ggttcccttc agcagtagac aggaacttct
      781 ggaggaggcg gatatatagt ttgtgtaagc atattaactc catatgttat atattgaaga
      841 acttctgctt tacactatcc taaactggat cggtttaatg taaaaaaacg atactttttg
      901 ggggggggca atttttcctt aaggtatgaa aaaaggagct gaagctatat aatagctatt
      961 aatgccactg gtatcagtga atcatgtata taaaaaactg tgttaaggag gctgtccatt
     1021 agcggttttt tattcttctg gaaccgcggc atctttgcgt atctttttgt cttctcaccg
     1081 tctggtcggt gttttgctga gactgctaat ttcctgtctt tgctagtgct gtactggtac
     1141 tgcccaatca tgattggcgg gggcgggcgg agttgagacc gcagacacgt cgtatgcaag
     1201 aacgtgctgc ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccagccg
     1261 ttactgattt tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct
     1321 ttcagagttt cctgaacaaa agttttagct gaatctttat cggcg
//
LOCUS       DAJTUM010000269         1364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTUM010000269 DAJTUM010000000
VERSION     DAJTUM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            598..882
                     /locus_tag="OO471_004318"
     CDS             598..882
                     /locus_tag="OO471_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6245303.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            882..1157
                     /locus_tag="OO471_004319"
     CDS             882..1157
                     /locus_tag="OO471_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6245304.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          365 a          342 c          340 g          317 t
ORIGIN      
        1 taatcgacaa caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat
       61 gtgcctgaca gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca
      121 ttaagtatca cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata
      181 aaatctcgta gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact
      241 ggtttgtact ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg
      301 gccctcacgg cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac
      361 cactccgacc gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc
      421 ggagggggac tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt
      481 acacctgtgc tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg
      541 ccttgttccg tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg
      601 caaatgaaaa acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct
      661 atggctgata aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc
      721 aaacaggcgc tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa
      781 gccctggacg atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg
      841 gacagcctca gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca
      901 gtaaggcact ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg
      961 ctgccgccag aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac
     1021 gtatgggaga acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg
     1081 agtacgaaat ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca
     1141 cacgagagaa caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat
     1201 tctggagcat tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat
     1261 aaaataaatt acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct
     1321 ccgggctggc aaataaccca tcagaaacgt tattgccaga catt
//
LOCUS       DAJTUM010000270         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTUM010000270 DAJTUM010000000
VERSION     DAJTUM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(41..100)
                     /locus_tag="OO471_004320"
     CDS             complement(41..100)
                     /locus_tag="OO471_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6245305.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="OO471_004321"
     CDS             complement(339..1247)
                     /locus_tag="OO471_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245306.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            complement(1281..>1356)
                     /locus_tag="OO471_004322"
     CDS             complement(1281..>1356)
                     /locus_tag="OO471_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6245307.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAJTUM010000271         1338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTUM010000271 DAJTUM010000000
VERSION     DAJTUM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(209..364)
                     /gene="hokD"
                     /locus_tag="OO471_004323"
     CDS             complement(209..364)
                     /gene="hokD"
                     /locus_tag="OO471_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6245308.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(592..1257)
                     /locus_tag="OO471_004324"
     CDS             complement(592..1257)
                     /locus_tag="OO471_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6245309.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          315 g          350 t
ORIGIN      
        1 gtccatccct cttctccctg cgcgctactt aagtgagtcg attctatctg cgaaggtgcg
       61 cgaatcaaat tcaccggaaa taaacaacaa aaaacccgcc gaaacgctga gagatcacag
      121 cgatgggcgg gttaagtgcg ggtgcgttga ggatgcctga cacatcagag gtgggcgggg
      181 atgggatcag ctccccaccc ggtcactctt acttcctgga ttcgtagtct acgaagacag
      241 cgacctccgt ctggccggtt cggattcgta cctcacagag gtctttcctc gttaccagtg
      301 ccgtcactat gacggttaaa cagatgacga tcagggcgat taacatcgcc ttttgctgct
      361 tcatagcctg cttctccttg cctttcggca tgtaagaggc taacctacat gtgcaaagca
      421 tgaaattggc ctcagattaa tgttaagcgt cttgccggac gcgtaatgtt aactggggct
      481 tttctctatc tgccttttgg tgttcatgcc tgaagcagat agcctcaagc acccgcagtc
      541 attctactta cctcgccaat atgaaatcaa tcagaaaggt gccccataaa atcactcctt
      601 ctcttcttta ccgtagtgga gttgaccaat tttgataaga gggcgtccct gagatttgcg
      661 gtgtagattg gtatcgcgca gagaatacac acagccacaa tattcctgct gatagaattt
      721 ttcgcgcttg ctgatttcaa tcatacggga cgagccgccc tgcttgcgcc agttataatc
      781 ccagtacacc atgcccggat aatgcgcgac ggctcgccgc ccacagtcgt taacctgctg
      841 catatttttc cagcgtgaaa tgcccagtga actgctgatc acactgaaac cattttcagc
      901 ggcgtacagc gctgtccgct caaaacgcat gtcaaaacac atggtgcaac ggatccctcg
      961 ttcgggctcc cattccattc ctttggctcg ttcaaaccag ttgtctgtgt cgtaatcagc
     1021 atcaataaac ggcacgccgt gttgttcagc aaagcgaata ttctcatcct tacgaattaa
     1081 atactctttc tgaggatgaa tgttcgggtt gtagaaaaag atggtgtagt cgatttccga
     1141 ggcctgaagc gcctccatca cctcaccgga acatggagca cagcaagagt gcagtagtag
     1201 tttgtttgcc ccgtttggga gctccaattt gggccgtttg aaatcagcaa ctgtcataaa
     1261 tatgtttatt gaggtcataa aaatatcaaa gagtgtagca ttagaacggg gctatcggaa
     1321 acagatgtgt gactgctc
//
LOCUS       DAJTUM010000272         1333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTUM010000272 DAJTUM010000000
VERSION     DAJTUM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..312
                     /locus_tag="OO471_004325"
     CDS             <1..312
                     /locus_tag="OO471_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6245310.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
     gene            315..>1333
                     /locus_tag="OO471_004326"
     CDS             315..>1333
                     /locus_tag="OO471_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6245311.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILT"
BASE COUNT          234 a          354 c          355 g          390 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg
      301 caaggagctt aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta
      361 tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg
      421 acatgtttga tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc
      481 tcctggcgat tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgttaa
      541 aacgcattgt tgacctgccg atgaaactgg tggggcataa accaggcggg ctgggctacg
      601 tcggcgtgct ggcagctatg attatggcga gcctttctgg ttctgccgtt gcggataccg
      661 ccgccgttgc cgctctactg gtgccgatga tgcgcagcgc caactacccg gtcaaccggg
      721 cggcggggct gattgcctct ggcggcatta tcgcgccaat tattccccct tccattccgt
      781 ttattatctt cggtgtttcc agcggattat ccatcagtaa gctgtttatg gcgggcattg
      841 cccctggcat aatgatgggc gcaacgctga tgcttacctg gtggcggcag gccagtcgcc
      901 ttaatttacc ccgccagcaa aaagcaacga tgcaggaaat ctggcactct tttgtctccg
      961 gtatctgggc gctgtttctt ccagtcatca ttattggcgg gtttcgctcc ggtctgttta
     1021 cccctaccga agccggggcc gttgctgctt tctatgcgct gtttgtcgcc acagttattt
     1081 accgtgaaat gaccttcgcc acactttggc atgtactcat tggcgcggcg aaaaccacct
     1141 cagtggttat gtttctggtg gcctcggcac aagtttccgc ctggctgatt accattgctg
     1201 aactgccgat gatggtttct gatttactgc aaccgttggt cgattcaccg cgtctgctgt
     1261 ttatcgtcat tatggtggct atcctgattg tcggcatggt catggattta acgccgacgg
     1321 tattaattct tac
//
LOCUS       DAJTUM010000273         1301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTUM010000273 DAJTUM010000000
VERSION     DAJTUM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO471_004327"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO471_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6245312.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          364 a          235 c          292 g          410 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc a
//
LOCUS       DAJTUM010000274         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTUM010000274 DAJTUM010000000
VERSION     DAJTUM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            34..435
                     /locus_tag="OO471_004328"
     CDS             34..435
                     /locus_tag="OO471_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6245313.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO471_004329"
     CDS             447..818
                     /locus_tag="OO471_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245314.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1294
                     /locus_tag="OO471_004330"
     CDS             805..>1294
                     /locus_tag="OO471_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245315.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLT"
BASE COUNT          306 a          293 c          401 g          294 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acac
//
LOCUS       DAJTUM010000275         1284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTUM010000275 DAJTUM010000000
VERSION     DAJTUM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(1..265)
                     /locus_tag="OO471_004331"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OO471_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO471_004332"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO471_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6245316.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1171..1284)
                     /locus_tag="OO471_004333"
                     /pseudo
     CDS             complement(1171..1284)
                     /locus_tag="OO471_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          379 a          319 c          202 g          384 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca ggactaacag
     1201 ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt
     1261 agtgttgtac gaataccaga gcca
//
LOCUS       DAJTUM010000276         1166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTUM010000276 DAJTUM010000000
VERSION     DAJTUM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..846)
                     /locus_tag="OO471_004334"
     CDS             complement(<1..846)
                     /locus_tag="OO471_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6245317.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
BASE COUNT          297 a          293 c          278 g          298 t
ORIGIN      
        1 ttttaaagaa agtaattgtt ccacagtcat atttttaatt gcgccccggt taacaagagt
       61 ccatccctgt ttttccagat aaaaccggaa agtctccagg gtacagacca gtgcgccatc
      121 aggaacggtt tcggtgaatt cgacattgcc gaatttgtcg aagtgaacaa ccagagtgcg
      181 accatcaccc ggaatcatct tgtctgcagg tggggtgtta ttctggcgca gttcggcctc
      241 catgcggtcg aactcagcaa tgtaggcttc cttgaatgcg gcggcttttt tgccagtgaa
      301 gcccatcacc aggaaaacga agccgttttt ggtgatttgg tacattgggc gtttttcgcc
      361 tttggcgtcg gtataggtga cgggcttaaa attaagccgg ttaaactccg gcgagcattc
      421 gagagtttcg atcttctgaa taacgttttt gtgcattttc cggaagaact ccgcaaccgc
      481 aacagacgta gtgacagcgc gaccattttc gatggttgcg tcagggtgag aaagggtaag
      541 gatagtagcc atgatggcag cctcgatagt cagtttaaat gactcaccac cagaggtagc
      601 aatctcatgg gtggtgagac gcacagggtt gctacaaccg gtgactaccg aaaccggcca
      661 gccttgcggc tgccctgcac gccccaccat aatctgaatg tggctgtgct tgacgcataa
      721 aaaaaccgcc tgagcgcggt tatgcgcagt agtcatcgtc ggggtagcaa tcccggcacc
      781 cgttttatga ggtgcaggtg cactataatt ccacccgttc tggttttcaa tagctacatt
      841 caacattttc tctttccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc
      901 tccaggtgac gttaaccagt taacaattac agttggcttt cccacccaaa ccaataagga
      961 ctaacatgac ttttaactgt gccgcgtgcg catagccgca ttcaccgcat cacaaaattc
     1021 actttaaaaa gggcggacat cagccagcaa ttaaaccgat gccgccaact ggtacttcac
     1081 acagcaatgt cgttatttac aaccggaagc gcactcccac catttaaatt tcacagacaa
     1141 gaccgactct ttatggatac cggaaa
//
LOCUS       DAJTUM010000277         1150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTUM010000277 DAJTUM010000000
VERSION     DAJTUM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            115..>1150
                     /gene="cadB"
                     /locus_tag="OO471_004335"
     CDS             115..>1150
                     /gene="cadB"
                     /locus_tag="OO471_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6245318.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGG"
BASE COUNT          243 a          253 c          296 g          358 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta
//
LOCUS       DAJTUM010000278         1121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTUM010000278 DAJTUM010000000
VERSION     DAJTUM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(157..639)
                     /locus_tag="OO471_004336"
     CDS             complement(157..639)
                     /locus_tag="OO471_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6245319.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(630..932)
                     /locus_tag="OO471_004337"
     CDS             complement(630..932)
                     /locus_tag="OO471_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6245320.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          326 a          272 c          229 g          294 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcagactcag actttataaa acaaggtatt
      181 aggtgccaca actgactgaa caaaaccaaa acgctcataa aatgctctgg cgtcatcatc
      241 aattgcatgt accataacag ccttaatgcc aacctgatca gccagattga caacccggtg
      301 aatggcatca cttagtagcc atttaccgaa accatgcccc tgggtacaaa catcaacagc
      361 cagacgccct aacagaacaa cagggacagg attgggcatg ttatgccgca aactgcgacc
      421 aagctcggca tgagtgacgg aaccagtagc taatgagtag tatcccacaa tacgtaaagt
      481 atcctccaag caaataacga acgtgcgaga agcattgagc atctggtttt tcatagcccg
      541 accacgtaac cagttactta gcacctcatt tccacagtca aatggctgaa gtatatgctc
      601 atccgtcaac aatgctggcg cggtgacatt tatttccatt caggctttac cgccatcaga
      661 cgttcacgcc cttctgcatt ctgcacagga gcttccagtt gcgtaataaa cgaatcataa
      721 cgttcgttat ccagcacaaa taaacgttga tccaggataa tgtttttagc ttcctggtac
      781 gccagttcca tgatgaaatc ggtacggttc ttattgacca agctcgcagc ataatcaata
      841 acggctcgtt cctcatcagt tgcccggatg ttaatctgat gttctcgcgg tgttttacgg
      901 acgcttgcag ctgtagacat aaaaacctcc attggtaaca atttgctatg caattaatgt
      961 tatcgcaaat ggtaacagaa cgctatacag aatcacttac gggaaaagcg atcccctgat
     1021 aaaaacaatg gcagagacct gaacattgtg acgatgatct gtgtcggcgc acaaatcatg
     1081 tctgattacg gccgtttaat gtgattttga taatgaaatg c
//
LOCUS       DAJTUM010000279         1109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTUM010000279 DAJTUM010000000
VERSION     DAJTUM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..1019
                     /locus_tag="OO471_004338"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO471_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAJTUM010000280         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTUM010000280 DAJTUM010000000
VERSION     DAJTUM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            55..>1100
                     /locus_tag="OO471_004339"
     CDS             55..>1100
                     /locus_tag="OO471_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6245321.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTUM010000281         1087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTUM010000281 DAJTUM010000000
VERSION     DAJTUM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..565
                     /locus_tag="OO471_004340"
                     /pseudo
     CDS             1..565
                     /locus_tag="OO471_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          337 a          238 c          225 g          287 t
ORIGIN      
        1 acagcaccta atcaggtctg ggtcgcagat tcagttgtcc aggccttccg aaatcaaccg
       61 actgaaggcg ctggtcgcga aactgcagcg tatgcagttc ggtaaaagct cagaaaaact
      121 tcgcgcaaaa accgaacggt ggatacagga agcacaggag cgaatcagcg cacttcagga
      181 ggaaatggcg gaaacactgg gtgagcaata tgacccggta ctgccatctt ccctgcgcca
      241 gtcttcagcc cgtaaaccgt taccagcctc acttccccgt gccccccggg tcatccggcc
      301 ggaagaggaa tgctgtccgg cctgtggtgg tgaactcagt ccactgggat gtgatgtgtc
      361 agagcagctg gagcttatca gcagcgcctt taaggttatc gaaaaacaac gccctaaact
      421 ggcctgtcgc cggtgcgacc atatcgtgca ggcaccggtg ccttcaaaac ccattgcacg
      481 cagttatgcc ggagcggggc ttctggccca tgttgttacc ggaaagtatg cagaccatct
      541 gccattatac cgccaaagtg atctactatt ccacacagct atttagctct ctttttgttg
      601 agctcccatg attctttcat ttattgttac taattatttt atgttatcat gatgttagaa
      661 cacgctctgc tctgtgtctc tattttatct atcatattct gaggattaac tacataacaa
      721 ggcgcaagat atatatacaa aaacaaataa taccgttgca ataattcctt ttagtaaagg
      781 aggtgtgatt aatttcaggg gatttcagag taaaaattgt acaacatatt aaaatatgtt
      841 catttcgtta taatacacac aaggaaatat taagttagat aatatattct aaataaccat
      901 agataaaaac gcacataatg gggaatattt tatgaaaata cctgaagcag taaatcatat
      961 taatgttcag aataatattg atcttgttga tggaaaaacg aatccgaata aagcaacaaa
     1021 agcattacaa aaaatatatt acgcgtaaca aactcatctt cttctggcat aagtgaaaaa
     1081 catttag
//
LOCUS       DAJTUM010000282         1080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTUM010000282 DAJTUM010000000
VERSION     DAJTUM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(19..822)
                     /gene="dkgB"
                     /locus_tag="OO471_004341"
     CDS             complement(19..822)
                     /gene="dkgB"
                     /locus_tag="OO471_004341"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6245322.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(985..1061)
                     /locus_tag="OO471_004342"
     tRNA            complement(985..1061)
                     /locus_tag="OO471_004342"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Asp,seq:gtc)
BASE COUNT          283 a          253 c          245 g          299 t
ORIGIN      
        1 aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc taaccaggcg
       61 gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct gtaaattttg
      121 tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta ctgagtaacc
      181 ttcccccata gcccacgcca gaatcacttg tgccggaatc gcattgtgtt tagctgcaat
      241 acgagcaata acctcatctt tcagtgcctt accataagcc agcgtcatat aggaagtgat
      301 atgaataccg tgctgtttag cccaggcaac cactttacgg ttttgcagat aaggagagag
      361 ttcaatctgg ttggtagcga tgttttcagc gccaacagca gcaatcactt tttccatcaa
      421 tgggatcgtg aagttggaaa taccgatctc acgcgtcagc ccttgttttt tggcttccag
      481 cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc agtggattag
      541 cgtcagatca acataatcgg tacgcaattt ttgcaggctc tctttcagac tcgggatcaa
      601 tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt catgacgtgg
      661 cacgccactt tctgcaatcg cctgacctac tgcggcttcg ttatcataga tttgtgcggt
      721 atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc
      781 tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc tcttttaatg
      841 ctattttcgt taactgaggt gattatgggc ttcagactgt tgcgggaaaa gagcgtaaaa
      901 cgcagaggat ttttgcgatt ctagcaataa tagatatcca gacgcaaaaa aacatcgagc
      961 gttaactcga ggcttcttaa taagtggcgg aacggacggg actcgaaccc gcgaccccct
     1021 gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc ttttacaacc
//
LOCUS       DAJTUM010000283         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTUM010000283 DAJTUM010000000
VERSION     DAJTUM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..199)
                     /locus_tag="OO471_004343"
     CDS             complement(<1..199)
                     /locus_tag="OO471_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HCR6245323.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLP"
     gene            385..1068
                     /locus_tag="OO471_004344"
                     /pseudo
     CDS             385..1068
                     /locus_tag="OO471_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          331 a          251 c          246 g          240 t
ORIGIN      
        1 ggggaagttt tccgccccga taaaaaaact ccagttcatc tgatgacagt gttttccagt
       61 tgttttcagc caaccatttc atttgtttcc ggaatgtctc gggagaaagt gtaaccagtc
      121 ctgggcaatg gctgacatga tgatacatta ataccggaac atgatttgct ttaaacaaaa
      181 tgccccctcc gtttaaaatt taaagtttct ggcacccagt aagtagagat aaaaggaaag
      241 cctaataaag gtggtgaact ttatatacta ctaaatcaaa catcaacaat ttataaacaa
      301 taatatatta tcatatcttt taaaaacggc tcactaagaa ccttgaggtg gcacactaaa
      361 tttagctacc tgaacagagg tgatatgctc acctcagaac aacacaggtg ccataatgaa
      421 aaaagaaatt tcagcgcaga gtttaaacgc gaatccgctc aactggtcgt tgaccagaaa
      481 tacaccgtgg cagatgcagc cagcgctatg gatgtcggcc tttccacaat gacgcgatgg
      541 gtgaaacaat tacgtgatga gcggcaggga aaaacaccaa aagcctcccc cattaccccg
      601 gaacaaattg aaatccgtga gctcaggaaa aagctacaac gtattgaaat ggaaaatgaa
      661 atattaaaaa ggctaccgcg ctcttgatgt cagactccct gaacagtttt cgataatcgg
      721 gaaactcagg gcgcgttatc ctgtggccac tctctgccat gtgttcgggg tccatcgcag
      781 cagctacaaa tactggaaaa accgtcctga aaaaccagac ggcagacggg ctgtattacg
      841 cagccaggta cttgaactgc atggcatcag ccacggctct gccggagcaa gaagcatcgc
      901 cacaatggca acccagagag gctaccagat ggggcgctgg cttgctggca gactcatgaa
      961 agagctgggg ctggtcagtt gccagcagcc gactcaccgg tataagcgtg gcggtcatga
     1021 gcacgttgct atcccgaatc atcttgagcg acagttcgcc gtaacgga
//
LOCUS       DAJTUM010000284         1058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTUM010000284 DAJTUM010000000
VERSION     DAJTUM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..550
                     /locus_tag="OO471_004345"
     CDS             <1..550
                     /locus_tag="OO471_004345"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245324.1"
                     /translation="DAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQA
                     IQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLT
                     GSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEV
                     ETALFNARALALKIYSMSGWNL"
     gene            777..>1058
                     /locus_tag="OO471_004346"
     CDS             777..>1058
                     /locus_tag="OO471_004346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6245325.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          309 a          199 c          255 g          295 t
ORIGIN      
        1 cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa ctcagcatga
       61 caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg gtgtgacaga
      121 taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct ccggcgggga
      181 gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca ctttgcttat
      241 tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag caaaaagcgg
      301 acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc gttatctgag
      361 aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca ttacagcaga
      421 agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg tgaatgaagt
      481 tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca tgtcaggctg
      541 gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt gtcagctcct
      601 gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg attcagcctc
      661 aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta gacagtgaga
      721 ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat ttgcatatgg
      781 aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat gtaaacggag
      841 gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa caatttacgg
      901 ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt ggtgttcata
      961 tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata gaacagtcgg
     1021 cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAJTUM010000285         1052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTUM010000285 DAJTUM010000000
VERSION     DAJTUM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..392
                     /locus_tag="OO471_004347"
     CDS             <1..392
                     /locus_tag="OO471_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245326.1"
                     /translation="AFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIP
                     LNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRN
                     SADNVDGVYYRPLQKLINGTWYNVASI"
     gene            395..940
                     /locus_tag="OO471_004348"
     CDS             395..940
                     /locus_tag="OO471_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6245327.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            964..>1052
                     /locus_tag="OO471_004349"
     CDS             964..>1052
                     /locus_tag="OO471_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6245328.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNT"
BASE COUNT          297 a          227 c          273 g          255 t
ORIGIN      
        1 cagcgtttta tttgtggggt aatccatcca ggcctgttgt tgcagagctt ggtgatgatt
       61 caggctggca ttttttcagc cagagaaacc cggataacag catagtgttc actgttaacg
      121 gacaggtaat tccgttaaat tacggaaact tcgatgcccg ctataaatat cgaacagagg
      181 gggtacagga tgtacggtat ggccatgaaa tgtattacag ccccggcagt aacaccgttt
      241 cgtggagatt ttgcgcacct tcgggacacg ggctgtcagg gatggcgata tcggataccg
      301 gccgtaactc agcggataac gttgacggtg tgtattaccg accactgcaa aaactgatta
      361 atggcacctg gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactgc
      421 gggtaatccg aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct
      481 tttttcagaa gacggaaaaa actggtatga agagctgaag aatttcgcca gcgacacaat
      541 aaaaatagct tacaccggag atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat
      601 cgagccacgc aatgccagcg ttatcgaagt tccggatatt accgctaacc gacggattac
      661 cgcgccgggt tactggtttt accgcaatga tgaatttgtc tttgactaca gactcaaagc
      721 ggaagatgag cgtgatgccc ttctggctca ggtcagtgcc cggacagggg aatgggaaga
      781 agacctgctg ctggggttaa tcagcgacga agataaagaa aagctgaaag cctgtcgtat
      841 ttacgcgaaa tcgctgcagg cgatggattt cagcaccatc actgataaat cctcatacaa
      901 cgccattgaa tggcccgcct ctccggaagg ttcttcctga tttaatttat cgcgagaaaa
      961 acaatgtctg tagtgatatc aggtgcgctg actgatggcg caggtatccc catgtccgga
     1021 taccatatta ttctgaaatc ccgggtaaac ac
//
LOCUS       DAJTUM010000286         1042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTUM010000286 DAJTUM010000000
VERSION     DAJTUM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(228..653)
                     /locus_tag="OO471_004350"
     CDS             complement(228..653)
                     /locus_tag="OO471_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245329.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(650..1033)
                     /locus_tag="OO471_004351"
     CDS             complement(650..1033)
                     /locus_tag="OO471_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245330.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          246 a          296 c          258 g          242 t
ORIGIN      
        1 tctcgcgtat ggttgattga caccacagtg cgaattcggc gaatgcgaga tcacggtacg
       61 attccgcacc acactaccaa tcaatacccc gggttttatt caggtaagca ggactgcgga
      121 tatccggtgt cgcgcctttg tgtcacgaac ggggtgggtg cgaaacacca gataaaatac
      181 aggttggcaa tacctctgaa cgccctgagc aggtcgggca tttcggatta agtactcgca
      241 cctccgcagt cctcaaacaa gtctggctgg tagctgtaaa cagacttcgt acataccgct
      301 ctgcaataaa tccccgtacc acaggcctca cagaacttct tcccatgaaa aaactgctca
      361 cacatcacac aatgccactc cagcacgacc ggtaatggcg ataaaaacat cgccatatcc
      421 tcaatgtaag ggtgggactt ctccggattc agcaccacac agtccgcctt ctgttgctca
      481 ctcagggcat gtaaatcatg ctcaaaccac gccccctgag catctgtctg cagaatcaac
      541 cggccatggc aggaaaggca gaaacactgc cggatatttc tgctgagcgc agagtcggca
      601 ctgataatgt gctcacccgg cgtgatcccc agccccgttt ttataccgtt cattctgctc
      661 ccccctcctc actgaagcgc ccggtctggc agtgtgtgca gtactgctcc ccataataat
      721 cgtggtgaca ttgcctgcag tgccagctgg ctttacgtac tacgggtaag gtatccggga
      781 cgaattgctg cagacgttta atcaaccgta tttctctgcg ttccggcctg acataagggc
      841 actcatgacc gtgctccgtc agcccttcgt cagcgtgttc aaaccagggc cgttcagtgt
      901 cgtattgcgg gtggtatctg agtgcactgc cgcaaaggtg gcaggtgtag ctatcgtaag
      961 gtgcagtctg tgcggtacgg gcaccggtca gacgtccgtt gccatcaagt gcgagaaaag
     1021 attttgcgta catagtatat gt
//
LOCUS       DAJTUM010000287          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTUM010000287 DAJTUM010000000
VERSION     DAJTUM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            82..>990
                     /locus_tag="OO471_004352"
     CDS             82..>990
                     /locus_tag="OO471_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6245331.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAJTUM010000288          964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTUM010000288 DAJTUM010000000
VERSION     DAJTUM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>964
                     /locus_tag="OO471_004353"
     CDS             <1..>964
                     /locus_tag="OO471_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR6245332.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAKTDARNAAIIAEAART
                     LPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPAPERVLGPRL
                     EHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPG
                     TNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLT
                     EVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKR"
BASE COUNT          209 a          314 c          246 g          195 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga
      241 cttacacgcc ggtgaagcta aaactgatgc tcgtaacgct gccatcattg ccgaagctgc
      301 ccgtaccctg cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact
      361 ctccatgctc tgcggcttcg atgatgatct tgccgcacag acaacgcagg ccagcaaccg
      421 tatccgcggc cttctgaccc agatacatcc ggcaccggag cgcgttctcg gtccgagact
      481 tgagcacccg gcggtactcg atcttctcca gcgatatccc tcaccagaaa aactcgcttc
      541 gctgggtgag aagaagctgg cagcccagct ctgcaaactt gcgcctcgtc tgggtaaacg
      601 ccttgcagca gacatagctc aggcactggc cgaacaaacc gtcgtcgttc ccggcacgaa
      661 tgccgctgcc gtagtactgc cacgtctggc actccagctc atcacgctgc gtaagcaaag
      721 agacgaggtg gcgcttgagg tagaacagcg agttcttgct caccctcttt acccggtcct
      781 gaccagtatg cccggagtcg gtgtcaggac cgcagccaga ctcctcaccg aggtcgcctg
      841 ccgcgccttc gcctctgccg cacatctcgc tgcttatgct ggccttgcgc cggtaactcg
      901 gcgatccggc tcgtcaatac gcggtgagca tccctcgcga cggggtaata aagctctcaa
      961 acgg
//
LOCUS       DAJTUM010000289          963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTUM010000289 DAJTUM010000000
VERSION     DAJTUM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
BASE COUNT          286 a          217 c          201 g          259 t
ORIGIN      
        1 gatacgtggg aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca
       61 tcacaaaatt cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa
      121 ggtaccaatc aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga
      181 caacccggtg tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc
      241 gcgttgtgtg cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac
      301 aagaaatttg tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag
      361 cgatgtcatc tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc
      421 aggctgaggt tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg
      481 aatatttgtc atctcattga tgagctgaca atctctaaaa gcaatgaggc tgcgctcaaa
      541 gcaaaactca cggagataga aaagtttaag ttccaaagtg aagactgcga acctcgctaa
      601 ttgattactg gaacgattgt gtatgccaga cagtgcaata ctagctctgg caagattacc
      661 atgtattttt gtccagaatg ttttgcgaag agagcaatat ctatattgca acctttccct
      721 atcgaggaag gcgataaata tacgaaaact caatgcccct cctgtaaaaa tgagtatctc
      781 atggactaca atttcaataa tgatttttga gtcacggtat tcagattttt aaagaacatg
      841 ccggatactc acccgtgtcc ggcgcacgca ctccacctca cccgtggaga actccttaat
      901 taccaacctt ggcttcgttg gttagccatt aacgctggta tgtaatcatt ctggcaatgc
      961 tta
//
LOCUS       DAJTUM010000290          905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTUM010000290 DAJTUM010000000
VERSION     DAJTUM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..781)
                     /locus_tag="OO471_004354"
     CDS             complement(<1..781)
                     /locus_tag="OO471_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6245333.1"
                     /translation="MRQRELAATAKAFMLDRPEVSLQELTEHLRSMAEQFLTDASDDY
                     WNGLEVATLVDEKSNLKELAATQALSLDQQKGIRLALEVLTAAQQRVDTGDTSGLIKL
                     IDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQERQSSVVFSSLVSSLLAEKVQTLKTS
                     SYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRDSMLASEVRPSTINKLLTKAKMCLDY
                     GLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEELERLLVRVE"
BASE COUNT          200 a          244 c          232 g          229 t
ORIGIN      
        1 actcaacacg gaccagaagt ctctccagct cctcgtctgt gaacgctctg cgcttggagt
       61 cgatatcctt ggtcagcttc atccgttcaa tagggttgcg gccctcaagt tgcccattca
      121 tcaggccata atcgagacac atcttcgctt tggtcagcag cttgttgatt gtcgatggtc
      181 tcacctcaga agccagcata gagtccctga cagccagcca cccagagcgg gacatcaggt
      241 ccatgtcttc aggcaggaac cgggagacag tattgagtga ggacgagagg tccttgtagc
      301 tggatgtctt cagggtctgg accttctcag ccagcagcga ggacactagg cttgaaaaaa
      361 ctacagatga ctggcgctcc tgtgtgaaaa ctgccggtct atccccctgc tcattattca
      421 gaattgacgt agagtcgcct atagttgagt catcagtaag gttattatca tcaatcagct
      481 taatcagacc agaggtatca ccagtgtcga ctctctgctg tgctgcggtc aggacttcca
      541 gcgctaacct tatgcccttc tgctggtcca gactcaaggc ttgcgtagcg gctagctcct
      601 tcaggttgga cttctcgtcg accagagtag cgacctcaag tccattccag taatcgtcac
      661 tagcgtctgt caggaactgc tcagccatag accggagatg ctctgtaagc tcctgtagtg
      721 agacttcagg cctatccaac atgaaggcct tagcggtggc tgcaagctct ctctgacgca
      781 ttgtggctac tctcctgtct gtgctgcggg tcgatactga aagaaacggt gtggtctgtc
      841 ccttgagtct gaagcggata gtatatcgac cattggctct gcggactaac tgaggcttag
      901 ctacg
//
LOCUS       DAJTUM010000291          880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTUM010000291 DAJTUM010000000
VERSION     DAJTUM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(338..>880)
                     /gene="aqpZ"
                     /locus_tag="OO471_004355"
     CDS             complement(338..>880)
                     /gene="aqpZ"
                     /locus_tag="OO471_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6245334.1"
                     /translation="FAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVA
                     AALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGAT
                     DKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVV
                     PIVGGIIGGLIYRTLLEKRD"
BASE COUNT          234 a          265 c          219 g          162 t
ORIGIN      
        1 cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata taaagccact
       61 ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc ccgggctctg
      121 gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa ccgggtcggg
      181 cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc tgccccggcc
      241 ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg taatgtcgaa
      301 gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt tccagcagag
      361 tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag aagaaccaca
      421 gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc gccgggttaa
      481 cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatag
      541 cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc actaacagga
      601 agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa ccgcctggtg
      661 aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac
      721 cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc tgggcaatta
      781 cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca atagtgaccg
      841 ccgggttaaa atgaccacca gaaatatgac caacagcgaa
//
LOCUS       DAJTUM010000292          877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTUM010000292 DAJTUM010000000
VERSION     DAJTUM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            24..>877
                     /locus_tag="OO471_004356"
     CDS             24..>877
                     /locus_tag="OO471_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6245335.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKR"
BASE COUNT          200 a          229 c          255 g          193 t
ORIGIN      
        1 gacggagtcg ccccgccctt ctgatgctcc cccgttttgc cgatattttt cagcagggaa
       61 accgctggct taactggctg gagaaacaac cggaaggggc agtccgtccg gtggtaattg
      121 aatcagtgac aaaaatcatg gcctgcggga ccacactgat gggctacacg cagtggtgtt
      181 gttcatcacc ggactgttgc catacaaaaa aggtctgctt ccggtgtaaa agccgctcct
      241 gcccgcactg cggggtgaag gctggcgcac agtggataca gtatctgctg agcctggtcc
      301 cctactgccc gtggcagcat attgtgttca cacttccctg ccagtactgg tccctggtgt
      361 tccacaaccg gtggttactg gcagagatga gccgcattgc agcggatgtg atactggaaa
      421 tctgccatca ggcagatgtg gagccgggga tattcacggt gatccacaca tgggggcgtg
      481 accagcagtg gcatccgcat atccatttat cgacaactgc cggtggtgtg acgtcgggcc
      541 acacctggaa aaatcttcat ttttacgccc gtaaggtgat gagcatgtgg cgctaccgga
      601 taacacggtt gctgtcacgg aaataccctt acctggtgat gccggatgcg ctggcagcag
      661 aaggaagcag taaacgggaa tggaatcgct tcctggacac gcattaccgc cgtggctgga
      721 atgtcaacgt atcccgggta atggataatg ccacccatgt ggcggtgtac tttggctctt
      781 acctgaaaaa gccaccggtg ccgatgagtc gtctggagca ttatgccggt caggatgaaa
      841 tcggtctgcg ttacaacagt caccgaacaa aacggga
//
LOCUS       DAJTUM010000293          856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTUM010000293 DAJTUM010000000
VERSION     DAJTUM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..446
                     /locus_tag="OO471_004357"
     CDS             <1..446
                     /locus_tag="OO471_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6245336.1"
                     /translation="PDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDA
                     SNSSPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFD
                     EVASASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(448..674)
                     /locus_tag="OO471_004358"
                     /pseudo
     CDS             complement(448..674)
                     /locus_tag="OO471_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          215 a          218 c          186 g          237 t
ORIGIN      
        1 tcccggatgc aatcaccttc accggcatct gttactcaca tcgcgtcttt gttgcgttga
       61 atgaaaagcc taatgtaaca gcaatacttt gtggtggtac gtatcgtgcc agaagtgatg
      121 ctttttacga tgccagtaac tcttcgccat tagactctct caatccgcga aaaatattta
      181 tttccgccag cggtgtgcat aatcactttg gcgtcagctg gtttaaccct gaagatcttg
      241 ccactaagcg taaagtgatg aaccgtggac tacggaaaat tttgctcgcc cgccacgcgt
      301 tgttcgatga agtggcctct gccagcctcg caccgatctc tgcatttgac gttctgatta
      361 gcgatcgtcc gttaccggca gattatgtta cgcactgcca gaatggttct gtaaagatca
      421 ttacacctga ttcagaagac gaatgactta ctgaaaaaac accacaatct tgttaaacat
      481 cgtcgggttg gactgattac gttgcacttt caccacatat tccagcttat ctatttggct
      541 tatcacctac tccagacgct ggtcatcctt gaccagtagc cagatatggc ttttgtcgca
      601 gtcctgaatc ggcaggcaga gaatgtcttc aacgttaaga gcgcgacggg caaaagccca
      661 caaaggtgag tcattacacc cggatggtaa acaggcactt ccttgttgta gaagccgtta
      721 tattgtccat aatgtctggg tttttaatgc atactgggta acaaccggac gaatcttcga
      781 atatcactgg cgctgccagc cggtttgaat tcatctcaca accctgcata gagcgaatct
      841 cctgcctgca cgtcac
//
LOCUS       DAJTUM010000294          851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTUM010000294 DAJTUM010000000
VERSION     DAJTUM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..851
                     /locus_tag="OO471_004359"
                     /pseudo
     CDS             1..851
                     /locus_tag="OO471_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          265 a          168 c          198 g          220 t
ORIGIN      
        1 aagttcgatt tcttgaaacg cggcttatgt atctaaaaaa gctgaaagcc ttagctcatc
       61 ccacgaaaaa gtgaaagtac tcaacgagct aaggcagttt tatcctcttg atgagcttct
      121 cagggctgcg gagataccgc gcagtacgtt ttattaccat ctaaaggctc tcagcaagcc
      181 tgacaagtat gcggacgtta aaaagcgtat tggtgagatt tatcacgaga atagaggccg
      241 atacggatac cgtagggtaa cgctgtctct tcatcgagaa gggaaacaga ttaaccataa
      301 agctgttcag cgcctgatgg gaaccctctc acttaaagca gcaattaagg tcaagcgata
      361 ccgctcttac agaggagagg tagggcaaac cgcccctaat gttctccaaa gagatttcaa
      421 ggctacgcgg ccaaacgaga agtgggttac cgatgttact gaatttgcag tcaatgggcg
      481 caagctgtat ttgtctccag taatagatct cttcaacaac gaagttattt cttacagcct
      541 ttcggaaaga ccagtgatga acatggttga gaatatgctc gatcaggcat tcaaaaagct
      601 taatcctcac gagcatcctg ttctgcactc tgaccaggga tggcagtatc gtatgagaag
      661 atatcaaaat atccttaaag aacatggtat taaacaaagc atgtccagaa aaggcaattg
      721 tctggataat gctgtggtgg agtgtttctt tggaacctta aagtcggagt gtttttatct
      781 tgatgagttc agtaatataa gcgaactgaa ggatgctgtt acggaatata ttgaatacta
      841 taacagcaga a
//
LOCUS       DAJTUM010000295          843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTUM010000295 DAJTUM010000000
VERSION     DAJTUM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..180)
                     /locus_tag="OO471_004360"
     CDS             complement(<1..180)
                     /locus_tag="OO471_004360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR6245337.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
     gene            complement(189..>843)
                     /locus_tag="OO471_004361"
     CDS             complement(189..>843)
                     /locus_tag="OO471_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6245338.1"
                     /translation="PPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKI
                     EQVRAKAAAYGRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTD
                     SVGQQRMAALHNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGI
                     DSFVLSGYPHLEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          179 a          261 c          229 g          174 t
ORIGIN      
        1 ccacagttcg ccgctggcgg agagcgtcca gaacgccgtc accactcctt ccggtgaagg
       61 caaaatacgc gtcgacagcc agccaaccga ggaggccagt tgccacaccg ccacgatgcc
      121 caccggtaaa aaccaggggg caacgcgcaa taaccacttc ttcactggcg ttgccatcat
      181 cgtgttcctt agctttgcgc gactttacgg gggataaaat catttgccac cgcttcgcct
      241 tgcggattca gcggctgcgg ctggggaatt tccggaatgg cgacatccag atgtgggaac
      301 agcaactcgc caacccgata cgcttcttcc agatgcgggt agcccgaaag cacaaagctg
      361 tcgatgccaa gcgcggcgta ttcgttgatt cgcgcggcga ccgtaggacc atcgcccacc
      421 agcgccgttc cggcaccgcc gcgcactaag ccaacgcccg cccataaatt ggggctgatc
      481 tccagattgt cgcgcttgcc gttatgtagc gccgccattc gctgttgtcc tacggaatcc
      541 gtccgggcga atgcggcttg tgctttggcg atagtctcat catcaagatg cgagattaac
      601 cgctcggcgg cctgccacgc ttcgtcgtta gtttcacgaa caatcacatg cagacgaata
      661 ccgaaacgaa ttttgcgccc atacgcggca gctttcgccc gcacttgttc gattttctct
      721 ttaacaagtt ccggcggttc gccccaggtg aggtagagat caacttgttc tgccgccagc
      781 tcctgggcga catctgacga tccgccaaag taaagcggag gataaggctg ttgaatcggc
      841 ggg
//
LOCUS       DAJTUM010000296          838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTUM010000296 DAJTUM010000000
VERSION     DAJTUM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            64..838
                     /locus_tag="OO471_004362"
                     /pseudo
     CDS             64..838
                     /locus_tag="OO471_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          235 a          210 c          221 g          172 t
ORIGIN      
        1 acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa cacaggtgct
       61 ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg aatccgctca actggttgtt
      121 gaccagaaat acacggtggc agatgccgcc aaagctatgg atgttggcct ttccacaatg
      181 acaagatggg tcaaacaact gcgtgatgag cgtcagggca aaacaccaaa agcctccccc
      241 attaccccgg aacaaattga aatccgtgag ctcaggaaaa agctacaacg cattgaaatg
      301 gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt cagactccct gaacagttct
      361 cgataatcgg gaaactcaga gcgcattatc ctgtggtcac actctgccat gtgttcgggg
      421 ttcatcgcag cagctacaga tactggaaaa accgtcctga aaaaccagac ggcagacggg
      481 ctgtattacg cagtcaggta cttgagctac atggcatcag ccacggttcg gccggagcaa
      541 gaagcatcgc cacaatggca acccggagag gctaccagat gggacgctgg cttgctggca
      601 ggctcatgaa agagctgggg ctggtcagct gtcagcagcc gactcaccgg tataaacgtg
      661 gtggtcatga acatgttgct atccctaact accttgaaag gcagttcgcc gtgaccgagc
      721 caaatcaggt gtggtgcggt gatgtgacct atatctggac gggtaagcgc tgggcgtacc
      781 tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg ctgggccatg tcgttctc
//
LOCUS       DAJTUM010000297          832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTUM010000297 DAJTUM010000000
VERSION     DAJTUM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..326
                     /locus_tag="OO471_004363"
     CDS             <1..326
                     /locus_tag="OO471_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6245339.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO471_004364"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO471_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6245340.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          242 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga gt
//
LOCUS       DAJTUM010000298          791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTUM010000298 DAJTUM010000000
VERSION     DAJTUM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..791
                     /locus_tag="OO471_004365"
                     /pseudo
     CDS             1..791
                     /locus_tag="OO471_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          187 a          221 c          224 g          159 t
ORIGIN      
        1 agcattcgcc tgaatttcgc agtgaagccc tgaagcttgc tgaacgcatc ggtgttactg
       61 ccgcagcccg tgaactcagc ctgtatgaat cacagctcta caactggcgc agtaaacagc
      121 aaaatcagca gacgtcttct gaacgtgaac tggagatgtc taccgagatt gcacgtctca
      181 aacgccagct ggcagaacgg gatgaagagc tggctatcct ccaaaaggcc gcgacatact
      241 tcgcgaagcg cctgaaatga agtatgtctt tattgaaaaa catcaggctg agttcagcat
      301 caaagcaatg tgccgcgtgc tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg
      361 gcggacaagg ataagcacgc gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc
      421 ggcttttacc cggtcaaaac agcgttacgg tgccccacgc ctgacggatg aactgcgtgc
      481 tcagggttac ccctttaacg taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag
      541 ggcaaaggcc tcccggaagt tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc
      601 agaaaatctg ttggagcagg atttttacgc cagtggcccg aaccagaagt ggccaggaga
      661 catcacgtac ttacgtacag atgaaggctg gctgtatctg gcagtggtca ttgacctgtg
      721 gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg acggcgcaac tgccctgcga
      781 tgccctgcag a
//
LOCUS       DAJTUM010000299          790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTUM010000299 DAJTUM010000000
VERSION     DAJTUM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..262)
                     /locus_tag="OO471_004366"
     CDS             complement(<1..262)
                     /locus_tag="OO471_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6245341.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNND"
     gene            complement(356..790)
                     /locus_tag="OO471_004367"
                     /pseudo
     CDS             complement(356..790)
                     /locus_tag="OO471_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
BASE COUNT          231 a          162 c          173 g          224 t
ORIGIN      
        1 catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc acagcttccc
       61 tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt cgcttacact
      121 gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc agagaaggaa
      181 ttgccttatt cataccaggg caaggtatta atttaaactg taataattta attttaggat
      241 gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg gcgttacagg
      301 aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca ataggtcaaa
      361 aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc gtcaaataat
      421 ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta atatccagga
      481 tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt tccaacccta
      541 aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc tcatagtttc
      601 ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac atttaaagga
      661 ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc gtttgccttg
      721 ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac ggaaacgaga
      781 tatctctccg
//
LOCUS       DAJTUM010000300          789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTUM010000300 DAJTUM010000000
VERSION     DAJTUM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..119)
                     /locus_tag="OO471_004368"
     CDS             complement(<1..119)
                     /locus_tag="OO471_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCR6245342.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCG"
     gene            complement(119..718)
                     /locus_tag="OO471_004369"
     CDS             complement(119..718)
                     /locus_tag="OO471_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6245343.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          197 a          210 c          187 g          195 t
ORIGIN      
        1 ccgcacgtcc ccgccagcct gtaatgcgcc agcacagaag tttcgggatt gtgattgcag
       61 tagccaggaa ttctgacggt gcacatctgc ccccgcgccg ctttacgtaa atccaccatt
      121 acgcaaactc cagtaactgc gcggccacat tttcgacttc ctccggagag gaaaatttac
      181 ggaacaggat ccagttccac agcacattca gtacagattt ataaacctgc tgaaactcgg
      241 tttcgtccat gttcgcaaat gcgatagatt ttgccctgcg cccacgacta ccatcaggat
      301 aaatatgctc ggtgtaaaat ccggcctgaa tggttaccca ctcgcggaaa gcgtcaaacg
      361 actttagcaa tgccgtatcc cgggttctgc atgtcgcaac tgtattaagg tattgctctg
      421 cggcatcact cagggctgga gtgtgttccc gacctactga ttcgcacagg taatcaacga
      481 agcctgatac cagttttcgt tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat
      541 caaatcccag ttgcaggagt ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct
      601 taaagtctgc gtgtatccac tcacctattt tgatttgatg caaaaaatcg caactctccg
      661 gcgtcgccgg gagaagtaat ccggaagagg tttgtttgac cagttgtata tgcgccatcg
      721 tagttctccg ctggcgcagt agaatgggtg ttcagcccgt tatgtagtat accagaatta
      781 atgccaata
//
LOCUS       DAJTUM010000301          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTUM010000301 DAJTUM010000000
VERSION     DAJTUM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            36..776
                     /locus_tag="OO471_004370"
                     /pseudo
     CDS             36..776
                     /locus_tag="OO471_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          226 a          159 c          195 g          196 t
ORIGIN      
        1 attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa tacccttttg
       61 aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac aggatcatct
      121 cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc tatgaaaaac
      181 atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca gagttgcgta
      241 ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct gctgctcact
      301 ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa gagcgcggag
      361 aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata tcagttgatc
      421 cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag gatcttgaaa
      481 ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa gccttagctc
      541 atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc ttgatgagct
      601 tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg ctctcagcaa
      661 gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg agaatagagg
      721 ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac agatta
//
LOCUS       DAJTUM010000302          766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTUM010000302 DAJTUM010000000
VERSION     DAJTUM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..87)
                     /locus_tag="OO471_004371"
     CDS             complement(<1..87)
                     /locus_tag="OO471_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6245344.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLD"
BASE COUNT          233 a          193 c          152 g          188 t
ORIGIN      
        1 atccaaccac tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc
       61 tgttgatatt ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga
      121 aagaaaaaag gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg
      181 aaaaaacacg cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc
      241 attcttaatt catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc
      301 ccaatgtttt tgctggcggg tccttttttc tacggtcctc ctccccggag gggggcattt
      361 cattattttt ctaatcgtta ctggtgagaa ggcggaagaa aaagccagtt cttcgcccct
      421 aaataacagc atgattccag tccatgcaaa atctgatttc cagctcacac ataccgagcg
      481 ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
      541 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgaatca tcagtcccct
      601 gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc
      661 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
      721 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctc
//
LOCUS       DAJTUM010000303          761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTUM010000303 DAJTUM010000000
VERSION     DAJTUM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(570..>761)
                     /locus_tag="OO471_004372"
     CDS             complement(570..>761)
                     /locus_tag="OO471_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6245345.1"
                     /translation="GQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDKSDYV
                     LAHGLNTDDQLAVGIVYQF"
BASE COUNT          206 a          122 c          199 g          234 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc c
//
LOCUS       DAJTUM010000304          759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTUM010000304 DAJTUM010000000
VERSION     DAJTUM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>759
                     /locus_tag="OO471_004373"
     CDS             <1..>759
                     /locus_tag="OO471_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6245346.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAJTUM010000305          758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTUM010000305 DAJTUM010000000
VERSION     DAJTUM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            79..351
                     /locus_tag="OO471_004374"
     CDS             79..351
                     /locus_tag="OO471_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245347.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            351..656
                     /locus_tag="OO471_004375"
     CDS             351..656
                     /locus_tag="OO471_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245348.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            656..>758
                     /locus_tag="OO471_004376"
     CDS             656..>758
                     /locus_tag="OO471_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6245349.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQAT"
BASE COUNT          241 a          163 c          189 g          165 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat
       61 gaaacagatg ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca
      121 caggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
      181 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
      241 ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc
      301 aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt
      361 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
      421 acgatattga gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg
      481 aacgcgaagc ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga
      541 gagcaaaaaa cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct
      601 ggcagcaacg agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga
      661 gccatacagc ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc
      721 cattaactgg atacgcacag ggcgtcttca ggcaacac
//
LOCUS       DAJTUM010000306          711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTUM010000306 DAJTUM010000000
VERSION     DAJTUM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..377
                     /locus_tag="OO471_004377"
     CDS             <1..377
                     /locus_tag="OO471_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6245350.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
     gene            377..>711
                     /gene="tnpB"
                     /locus_tag="OO471_004378"
     CDS             377..>711
                     /gene="tnpB"
                     /locus_tag="OO471_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6245351.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAG"
BASE COUNT          166 a          196 c          194 g          155 t
ORIGIN      
        1 accggcggcc taattttcct tatgaattta aaatcgcttt agtggagcag tcactgcagc
       61 ccggagcctg tgtggcgcag atcgcccgtg aaaacggaat caacgataac ctgctcttca
      121 actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat atgcctgcac
      181 tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg gcacaggaac
      241 cagagcagat aaatacaccg tccgacagtc tgtgctgtga gctggttctg ccggccggaa
      301 ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gatacttatc cgcgaaataa
      361 aagggagcag ccactgatga tatctttccc tgcaggttcg cgtatctggc tggttgcagg
      421 tatcaccgat atgcgaaatg gctttaacgg cctggtctca aaagttcaga acgtcctgaa
      481 ggatgacccg ttctccgggc atctgttcat cttccgcgga cgccggggtg accagataaa
      541 agtgttgtgg gctgacagtg acggactgtg cctctttacc agacgcctgg agcggggccg
      601 cttcgtctgg ccggtcaccc gtgacggcaa ggtgcacctt actccggccc agttatccat
      661 gcttcttgag ggcatcgact ggaaacaccc gaaacgaacg gaacgcgctg g
//
LOCUS       DAJTUM010000307          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTUM010000307 DAJTUM010000000
VERSION     DAJTUM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..673
                     /locus_tag="OO471_004379"
     rRNA            <1..673
                     /locus_tag="OO471_004379"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAJTUM010000308          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTUM010000308 DAJTUM010000000
VERSION     DAJTUM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            5..>693
                     /locus_tag="OO471_004380"
     CDS             5..>693
                     /locus_tag="OO471_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6245352.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          173 a          162 c          216 g          142 t
ORIGIN      
        1 gaccatgaaa aatattgcga caggaggcgt tctggagcgt atccgcagac tgaccccacc
       61 acatgtaacc gccccattca gaacggttgc ggagtggcgc gagtggcaac ttgctgaagg
      121 ccagaaacgt tgcgaggaga tcaaccgcct gaatcgtcag ttgcgggtgg aaaaaatcct
      181 gaaccgctcc ggcatccagc cgttgcaccg gaagtgttcg tttgcgaatt accgggcgca
      241 gaacgacggt cagcgacatg cactgagtca ggcgaaatcc atagctgacg aactgatgac
      301 aggctgtacg aattttgtgt tcagcggtaa gcccggcacc ggaaaaaatc atcttgcagc
      361 cgcgattggc aaccggctga tggcgaaggg gcgtagcgtg attatcgtca ccgtgtccga
      421 tgtcatgagc gtgttgcatg agagctacga caacggcaaa tccggtgaaa aatttttaca
      481 ggagctttgt ggtgttgacc tgctggtcct ggatgaaatt ggcatgcagc gggatacgaa
      541 aaacgagcag gtggtactga accagattgt tgatcgccgg acggcatcgt tacgcggtgt
      601 ggggatgctg acaaatatta accatgcagc gatgaataca cttctcggcg agcgggtgat
      661 ggatcgcatg gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTUM010000309          687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTUM010000309 DAJTUM010000000
VERSION     DAJTUM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            224..>687
                     /locus_tag="OO471_004381"
     CDS             224..>687
                     /locus_tag="OO471_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6245353.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALA"
BASE COUNT          187 a          140 c          165 g          195 t
ORIGIN      
        1 ggagcaagct gttttccttg ccaacaatct tgcaaaaaaa atgcgattgc taaaacaaaa
       61 gtgcaatttg cggcaatata gttattccat gaccgtacaa tcttgcattc agtgtaaata
      121 atcttgttta gctctcgcta aacgcggttg tataaccgca atacacgccg tattttatcc
      181 ggcatatatt gtttttgctt gctcatggaa tagggaggtt gtcatgtatc aacgctgttt
      241 tgataatgcc agtgaaacgc tgtttgtcgc cggtaaaacg ccacggcttt cacgttttgc
      301 atttagcgat gatccaaaat gggagtctgg acatcacgtt catgacaatg aaaccgagct
      361 gatttacgtc aagaaagggg ttgcaaggtt taccatcgat tcttcgttat atgtcgcaca
      421 tgcggatgac attgtggtga tagaacgcgg caggctgcat gcggtggcct ctgacgttaa
      481 cgatccggca acgacgtgta cctgtgcgct gtacggcttt cagtttcagg gggctgagga
      541 aaatcagtta ctgcaaccgc attcttgtcc ggtaattgcc gcggggcagg gaaaagaagt
      601 cataaaaacc ttatttaatg agctaagtgt gattttgccg caaagtaaaa atagccaaac
      661 atcttcgtta tgggatgcat tagccta
//
LOCUS       DAJTUM010000310          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTUM010000310 DAJTUM010000000
VERSION     DAJTUM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            103..>659
                     /locus_tag="OO471_004382"
     CDS             103..>659
                     /locus_tag="OO471_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6245354.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTUM010000311          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTUM010000311 DAJTUM010000000
VERSION     DAJTUM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(151..648)
                     /locus_tag="OO471_004383"
     CDS             complement(151..648)
                     /locus_tag="OO471_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6245355.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAJTUM010000312          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTUM010000312 DAJTUM010000000
VERSION     DAJTUM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            18..491
                     /locus_tag="OO471_004384"
     CDS             18..491
                     /locus_tag="OO471_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6245356.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTUM010000313          621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTUM010000313 DAJTUM010000000
VERSION     DAJTUM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            459..>621
                     /locus_tag="OO471_004385"
     CDS             459..>621
                     /locus_tag="OO471_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6245357.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDG"
BASE COUNT          214 a          113 c           97 g          197 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc a
//
LOCUS       DAJTUM010000314          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTUM010000314 DAJTUM010000000
VERSION     DAJTUM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>617
                     /locus_tag="OO471_004386"
     CDS             <1..>617
                     /locus_tag="OO471_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6245358.1"
                     /translation="KYNRLLSQRVAAYASEINRLKALVAKLQRMQFGKSSEKLRAKTE
                     RQILEAQERISALQEEMAETLGEQYDPVLPSPLRQSSARKPLPASLPRETRVIRPEEE
                     CCPACGGELSSLGCDVSEQLELISSAFKVIETQRPKLACCRCDHIVQAPVPSKPIARS
                     YAGAGLLAHVVAGKYADYLPLYRQSEIYRRQGVELSRATLGRWTGA"
BASE COUNT          146 a          172 c          176 g          123 t
ORIGIN      
        1 aaatacaacc gcctgctctc acagcgggtg gcggcttatg cttccgaaat caaccggctg
       61 aaggcgctgg ttgcgaaact gcagcgcatg cagttcggta aaagctcaga aaaacttcgc
      121 gcaaaaaccg aacggcagat actggaagca caggagcgaa tcagcgcact tcaggaagaa
      181 atggcggaaa cgctgggtga gcaatatgac ccggtactgc catctcccct gcgccagtct
      241 tcagcccgta aaccgttacc tgcctcactt ccccgtgaaa cccgggtcat ccgtccggaa
      301 gaggaatgct gcccggcctg tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag
      361 cagctggagc ttatcagcag cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc
      421 tgttgccggt gtgaccatat cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt
      481 tatgccggag cggggcttct ggcccatgtt gttgctggaa aatatgcaga ctatctgccg
      541 ttataccgcc agtcagaaat ataccgtcgt cagggcgtgg agctgagccg tgccacactg
      601 gggcgctgga caggtgc
//
LOCUS       DAJTUM010000315          599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTUM010000315 DAJTUM010000000
VERSION     DAJTUM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..>599)
                     /gene="ipaH3"
                     /locus_tag="OO471_004387"
     CDS             complement(<1..>599)
                     /gene="ipaH3"
                     /locus_tag="OO471_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6245359.1"
                     /translation="AVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNAL
                     ISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNN
                     QLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEET
                     EIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIR"
BASE COUNT          158 a          101 c          164 g          176 t
ORIGIN      
        1 ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc ggatcaaggc
       61 taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa aatatctcgg
      121 tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga agattagtac
      181 ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc tggttatttc
      241 ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc tgattgttat
      301 ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt tggttgttat
      361 ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg cgattgtcac
      421 aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg gcattctgag
      481 taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac agatttaaac
      541 gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg gagaccgct
//
LOCUS       DAJTUM010000316          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTUM010000316 DAJTUM010000000
VERSION     DAJTUM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
BASE COUNT          217 a          105 c          122 g          148 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 gggggggatg aaaatacctg aagcagtaaa tcatattaat gttcagaata atattgatct
      541 tgttgatgga aaaacgaatc cgaataaagc aacaaaagca ttacaaaaaa at
//
LOCUS       DAJTUM010000317          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTUM010000317 DAJTUM010000000
VERSION     DAJTUM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..466)
                     /gene="queE"
                     /locus_tag="OO471_004388"
     CDS             complement(<1..466)
                     /gene="queE"
                     /locus_tag="OO471_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6245360.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERA"
BASE COUNT          153 a          158 c          129 g          150 t
ORIGIN      
        1 tggctcgctc cagtgcctgt gacaacactt catagccgcc gcgcatgttc agctttggcg
       61 atacggtaac ccaggtattc ggtgtgcagc gtacttcatg agttccactg gtttcgatct
      121 ggcagctaaa accgttcttt tcgagcaggt cagtcagtgg cagcaaatca tgaatgcaag
      181 gctcaccacc cgtaatcacc acatgccgcg cggtatatcc ctgacgacca atgacagcca
      241 gcaagtcttc actgctcgcc gccccccact tatcactctc tttggtcttc gccagaatgc
      301 tgaaaaggga gacttcccga tcctcaagct tttcccaggt gtgtttggtg tcgcaccagg
      361 cacagccaac cgggcatccc tgtaaacgaa taaaaatggc gggaacgccg gtaaagtaac
      421 cctcaccttg cagggtctgg aacatctcgt taatcgggta ctgcatagca ttctctgtga
      481 agtggataat tgttaattat tgcagatcct gccgcaacaa tcatgtctta ttaacattct
      541 gttacatgca gatttaaaga aaaatacgaa acatatcgtt aataattaaa
//
LOCUS       DAJTUM010000318          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTUM010000318 DAJTUM010000000
VERSION     DAJTUM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..>581)
                     /locus_tag="OO471_004389"
     CDS             complement(<1..>581)
                     /locus_tag="OO471_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6245361.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISE"
BASE COUNT          158 a          142 c          102 g          179 t
ORIGIN      
        1 tttctgaaat agctttccag ccacctttag cggtgatttg tttattcagt tcctgagtga
       61 ccttctgaag agtatcggca gattttaaag cccccttctt catagcatca aataattcct
      121 gttcagtctt tccggttgct ttagcgaata cctgaaccat accaggaaca gcttcagcta
      181 gttgcccctt aagctcttca gccataatct gacccttacc agccatctgt gataatgctg
      241 tcagtgctcg cttaatctct tcttcacttc gccccatcag acgagaatag ccaatcatat
      301 ttctaaaaag ttcttcagaa ccttgaatgc ctagagactc tctagctgaa gcaccgaaac
      361 cagcaaactg tgattgcagt tcagcgatat ctaaacccgt tgtttgagct aatgactttg
      421 tgaaatcttt tacttgattt gcatctttag aaaatacgaa atcaattgaa cgttcagcac
      481 ttgcacgttg aaggccaact tccattactc gctgataagc ggatagtaca gcctgaaagc
      541 tgataagtac acctgctgtt ttaactgctg ctgaacttaa a
//
LOCUS       DAJTUM010000319          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTUM010000319 DAJTUM010000000
VERSION     DAJTUM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(137..>577)
                     /locus_tag="OO471_004390"
     CDS             complement(137..>577)
                     /locus_tag="OO471_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6245362.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          146 a          160 c           99 g          172 t
ORIGIN      
        1 agcacgttta acgtcctgtg ccgtttttca ggtataaaaa aaaccgcata aagcgggctc
       61 tttcaaatgt ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc gtacaagaat
      121 tgaagcagtt tttacgtcaa aaagcaataa cttttttctc tataccaaaa gccataacca
      181 ttggtttgta caaaataaat tctgccacct ttagccaatg ctcaatgcgt ctttcacagg
      241 ttcttaaact ccatttcggg tgtgcatcat tcagcagttc agccattttg catttggtca
      301 tcccccgccc ctcataccgt tgccgaagga cgctaatcaa tcctggatgc tctgccagca
      361 cctcacttat gacccgatca atacataacg cctctgcatc agtacaatgc gccagccagc
      421 tcttttgctt accgttaatc atatcccgca aaaaagcctc aagttcagac ttgttcagac
      481 ctgctttttt catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg
      541 tcaacatcag gttaaacata ttccccgtct taccgcc
//
LOCUS       DAJTUM010000320          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTUM010000320 DAJTUM010000000
VERSION     DAJTUM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..179)
                     /locus_tag="OO471_004391"
     CDS             complement(<1..179)
                     /locus_tag="OO471_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6245363.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITH"
     gene            complement(320..>573)
                     /locus_tag="OO471_004392"
     CDS             complement(320..>573)
                     /locus_tag="OO471_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6245364.1"
                     /translation="LCIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHP
                     EWCFRTCCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          157 a          156 c          137 g          123 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acg
//
LOCUS       DAJTUM010000321          572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTUM010000321 DAJTUM010000000
VERSION     DAJTUM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..>572)
                     /locus_tag="OO471_004393"
     CDS             complement(<1..>572)
                     /locus_tag="OO471_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6245365.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGG
                     LIIAAYQGEADGDKVIRLVRGQSYRGSRLG"
BASE COUNT          127 a          159 c          143 g          143 t
ORIGIN      
        1 tcccagtcgt gagcctctgt atgactgccc cctgacaagt ctgatgactt tatcaccgtc
       61 agcttctccc tggtacgcag caataatcag cccgccaatg tcagggtctc cccatctgtt
      121 gcggacagag ctcgccacga ttctgtaaat aatatcttct gtggttatat ttattttcac
      181 ccagccagtc agtctggata tgggccagta gcagtagcgg gtgtgataaa tggggccgtt
      241 aatgccgtaa aaagtaaggg atttggctct gtaccgcggt tctgttgtct cagggcgtgc
      301 atcagtccac cggatgctga gcaccccttc aaaccgtgtg tcgggtatga tgatgccgta
      361 ggggccagca acggaatatt cacctggcag cgcattcctt acccaggcca ggaaatcact
      421 cttagtgtca aaacggataa catcttcagg cagaaaagca cacccaaagc cgaatgcgcc
      481 gggtatcgcc agacggcctt ttgtccggtc gtaaatgtcg ctctgtgctt ccatcgtggc
      541 cgcacttttc agccccagat tatcccggga ct
//
LOCUS       DAJTUM010000322          567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTUM010000322 DAJTUM010000000
VERSION     DAJTUM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            153..>567
                     /locus_tag="OO471_004394"
     CDS             153..>567
                     /locus_tag="OO471_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6245366.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          143 a          156 c          142 g          126 t
ORIGIN      
        1 gctgcatccg actcttttat caacgcttac gtaattaagg gacacagcct aaatagctgc
       61 gcggaatagt agatcccttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc
      121 aaatcaaaac aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg
      181 acgaacgacg cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca
      241 gactgactgc catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc
      301 tctgctgcgc ccgttcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg
      361 agggactgaa atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca
      421 cactgttacg tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct
      481 ggagtacaga actgctggca ataaaaatca atgagataac cggctgccag ttaaatgccg
      541 gaaccgttcg ccgctggttg ccgtctg
//
LOCUS       DAJTUM010000323          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTUM010000323 DAJTUM010000000
VERSION     DAJTUM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..296
                     /locus_tag="OO471_004395"
     CDS             <1..296
                     /locus_tag="OO471_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6245367.1"
                     /translation="SAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGI
                     DDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(404..>565)
                     /locus_tag="OO471_004396"
     CDS             complement(404..>565)
                     /locus_tag="OO471_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245368.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          184 a          133 c          104 g          144 t
ORIGIN      
        1 gcagcgctca gtactccggc gaaataaagg gatacgatta cgatacatat accttctatg
       61 ccaaaaaagg tcagaaagtg catgtgagta tctctaatga aggcgctgat acttatctct
      121 tcgggccagg aatcgatgat tctgttgatt tatccagata ttccccagaa ctcgatagtc
      181 acggacaata ctcactccct gcttctggca aatatgagct gcgagtgctg caaactcgaa
      241 acgacgcccg taaaaacaaa actaaaaaat ataacgtcga tattcaaatt aagtaaatgt
      301 caaccgacgg ggactatccc cgtcatagac gttaacgccc cactcctctc tgtcgctcaa
      361 gaggttaatg acgaatagca gagaacataa tgtaaacaag gcatcactca cgacacataa
      421 ataacatctt tgtggtggtt tgattgattt ttccactccg cttaccatct acaccaaaat
      481 aacgatcatc acgacataat ttttctttaa catcgcaaaa tacaccgtta gcgaatgtaa
      541 attcgctggg atcgtagcct tgtaa
//
LOCUS       DAJTUM010000324          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTUM010000324 DAJTUM010000000
VERSION     DAJTUM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>555
                     /locus_tag="OO471_004397"
     CDS             <1..>555
                     /locus_tag="OO471_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6245369.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETV"
BASE COUNT          164 a          135 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcag
//
LOCUS       DAJTUM010000325          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTUM010000325 DAJTUM010000000
VERSION     DAJTUM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..269
                     /locus_tag="OO471_004398"
     CDS             <1..269
                     /locus_tag="OO471_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6245370.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>552
                     /gene="tnpB"
                     /locus_tag="OO471_004399"
     CDS             266..>552
                     /gene="tnpB"
                     /locus_tag="OO471_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6245371.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVA"
BASE COUNT          131 a          145 c          163 g          113 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gc
//
LOCUS       DAJTUM010000326          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTUM010000326 DAJTUM010000000
VERSION     DAJTUM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>548
                     /gene="ospC4"
                     /locus_tag="OO471_004400"
     CDS             <1..>548
                     /gene="ospC4"
                     /locus_tag="OO471_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6245372.1"
                     /translation="NFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRK
                     SKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIK
                     ASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRY
                     CNLYDVEYLLSKDGANYKVLEY"
BASE COUNT          210 a           63 c          105 g          170 t
ORIGIN      
        1 taactttaaa gaggttgtgg atgaagttag caggtatgtg catggtaatc aggggaaaac
       61 tgatgttcca atatttaaca ctaaagatat gaggcttgga attgggttgc atcttattga
      121 ttttataaga aagagtaaag atcaaggatt cagagagttt tgttataaca aaaatattga
      181 tcctgttagt ctggatagaa ttataaactt tgtgtttcag cttgagtatc atataccaag
      241 aatgctaagt acagataact tcaaaaaaat aaaactcaga gacatatctt tggaggatgc
      301 gattaaagca tctaattatg aagagattaa caataaagtg actgataaaa aaatggctca
      361 tcaggccctt gcttattctt taggcaataa aaaagctgat atagcacttt atttactatc
      421 taaatttaat tttacaaaac aagatgtcgc agagatggag aaaatgaaaa acaatagata
      481 ttgtaatctg tatgatgttg aatacttact tagtaaagat ggtgccaact ataaagtgct
      541 agaatatt
//
LOCUS       DAJTUM010000327          535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTUM010000327 DAJTUM010000000
VERSION     DAJTUM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(427..>535)
                     /locus_tag="OO471_004401"
     CDS             complement(427..>535)
                     /locus_tag="OO471_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6245373.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          138 a          124 c          110 g          163 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga gtcaagttcc
      181 cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat tccccggagg
      241 caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga aagagggacc
      301 atctccatct cattaaatgg accacgccag acaattcact gtcatgctga tgctgcactg
      361 tggggacaga atcactcatg atgacttcga taatcgacga cattattatt tggccgagct
      421 tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata aaaaatcaat
      481 catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac tattt
//
LOCUS       DAJTUM010000328          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTUM010000328 DAJTUM010000000
VERSION     DAJTUM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
BASE COUNT          141 a          114 c          104 g          173 t
ORIGIN      
        1 tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta ccctcatcgg
       61 taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt cagggaagta
      121 atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt tgctactaaa
      181 tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg ttatatccac
      241 atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt tgattgttta
      301 atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat atggttattt
      361 ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta gtgcgggtgc
      421 gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcct ggtctcttac
      481 tcctcaggtt cgtaagctgt gaagacagcg acctccgtct ggccggttcg ga
//
LOCUS       DAJTUM010000329          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTUM010000329 DAJTUM010000000
VERSION     DAJTUM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..252
                     /locus_tag="OO471_004402"
     CDS             <1..252
                     /locus_tag="OO471_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6245374.1"
                     /translation="TSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNA
                     QMVFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            273..>522
                     /locus_tag="OO471_004403"
     CDS             273..>522
                     /locus_tag="OO471_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR6245375.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          170 a          120 c          121 g          111 t
ORIGIN      
        1 acaagtatct taaaacgcgc aggtctgaga cacagaaaat cgtatcaatc caggcatacc
       61 tatgcatgct ggtcacttgc cgcaggagct aatcctagtt ttatcgcaag ccagatgggc
      121 cacacaaacg cacaaatggt attcaatgtt tacggagcat ggatgaaaga caacaatcac
      181 gaacagatag aacttcttaa cagaagacta tctgaaagtg tcccatgcat gccccataag
      241 aaagcggggt aaaataaaaa cttgcaaaat caattggttt acctttaatc cctgtcacgt
      301 tacgcgcgtg gcagaggcgt tacggattgc tgaaaccgca acggacagac ggcggtcatc
      361 ggctgttcaa cgatgccgat attgaccgga tccgcgagat caaacgctgg atcgacaacg
      421 gcgtgcaggt cagcaaagtt aaaatactgc tcagtaatga aaatgttgat gtgcagaacg
      481 tctggcgcga tcagcaagaa acattactga cttacctgca aa
//
LOCUS       DAJTUM010000330          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTUM010000330 DAJTUM010000000
VERSION     DAJTUM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(30..518)
                     /gene="capU"
                     /locus_tag="OO471_004404"
                     /pseudo
     CDS             complement(30..518)
                     /gene="capU"
                     /locus_tag="OO471_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
BASE COUNT          160 a          117 c           98 g          143 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccta aattcgtgcc ctttttccgg gcgcaacatt
       61 cctacctgag caattacggg gcatcctctt cgggacgcca gccaggaaag aacactcgga
      121 ggcaaactgt ttcgggaatc aacgtataat tcctggaaat caaagccagg aggcacaaca
      181 gtaacccgag ttcgacatcc ttcctgctcc agatgtgtct tcatatttgt tccgggaaca
      241 atcacctcat cgcaaaaatg atttattgaa aaaaattttg tttttcgtgt caaatatgtc
      301 ttttgcctga taattctgaa tggatgtttc caggtaaaaa accgtaccaa accaacaata
      361 ttactatcat gtccactgtg acaaacgatt gcatcgggct gaaaactatg aactattccg
      421 agtaatctcc atacagtagg aatgtgaaga ctgtttcgaa ataacgcgaa tgtgatatca
      481 attccaaact tactggcttc aaaagcaatt ttgctgtt
//
LOCUS       DAJTUM010000331          512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTUM010000331 DAJTUM010000000
VERSION     DAJTUM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..195
                     /locus_tag="OO471_004405"
                     /pseudo
     CDS             1..195
                     /locus_tag="OO471_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            295..>512
                     /locus_tag="OO471_004406"
     CDS             295..>512
                     /locus_tag="OO471_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245376.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFT"
BASE COUNT          175 a          106 c          101 g          130 t
ORIGIN      
        1 ctacgtaatt aaaacagaaa ataaagaaac cccattcact gtagttgata tcgacggtct
       61 atcaggaaac tttaaaactc ttgttgaagg tgccacaaaa atgtgcctag tttacatcgg
      121 aaataatctg cccgcagaaa aaaagccgaa tttctggcaa atctgattgc aatgaaatta
      181 aaaggtgaaa tctgaaaaaa agaaagcctg cacagtgtgc aggcctgagt gaagaacctg
      241 ggacatttat tcatcactcg cagtaatttt aatctgagtt gaggttaaaa aacaatgagc
      301 acaaaaccac tcttcctgtt acggaaagcg aaaaaatcat ccggtgaacc tgacgtcgtc
      361 ctgtgggcaa gcgacgattt tgaatcgacc agcaccactc tggactatct gctcgttaag
      421 tcaggtaaaa aactgagcaa ctattttaaa gctgttgcca caaattttcc tgtcgttaat
      481 gaccttcccc ctgaaggtga gatcgatttt ac
//
LOCUS       DAJTUM010000332          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTUM010000332 DAJTUM010000000
VERSION     DAJTUM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            1..493
                     /locus_tag="OO471_004407"
                     /pseudo
     CDS             1..493
                     /locus_tag="OO471_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a          120 c          154 g          110 t
ORIGIN      
        1 agaagccgtt gaatatggac gggcaaaaaa gtggatagcg cacgcgccct tattgcccgg
       61 ggatggggag taagcttagt cagccgttgt ctccgggtgt cgcgtgcgca gttgcacgtc
      121 attctcagac gaaccgatga ctggatggat ggccgccgca gtcgtcacac tgatgatacg
      181 gatgtgcttc tccgtataca ccatgttatc ggagagctgc ccacgtatgg ttatcgtcgg
      241 gtatgggcgc tgcttcgcag acaggcagaa cttgatggta tgcctgcgat caatgccaaa
      301 cgtgtttacc ggctcatgcg ccagaatgcg ctgttgcttg agcgaaaacc tgctgtaccg
      361 ccatcgaaac gggcacatac aggcagagtg gccgtgaaag aaagcaatca gcgatggtgc
      421 tctgacgggt tcgagttctg ctgtgataac ggagagagac tgcgtgtcac gttcgcgctg
      481 gactgctgtg atc
//
LOCUS       DAJTUM010000333          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTUM010000333 DAJTUM010000000
VERSION     DAJTUM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            120..>491
                     /locus_tag="OO471_004408"
     CDS             120..>491
                     /locus_tag="OO471_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6245377.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAE"
BASE COUNT          168 a          105 c          121 g           97 t
ORIGIN      
        1 gatcccacta agaataaatc tccttacctc acaacacgac aagcctgcat tgcggctctt
       61 cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg gcataacaga ggaaagaaaa
      121 tgtcactctt ccgcagaggt gaaatatggt acgccagttt cacattgccg aacggtaaaa
      181 gatttaaaca gtctcttgga acaaaggaca aaaggcaggc gacagaactc catgacaagc
      241 taaaggctga agcatggcgg gtcagcaaac ttggtgaaat acctgatata acgttcgagg
      301 aagcgtgtgt caggtggctt gaagagaaag cacataaaaa atcactggac gatgacaaaa
      361 gccggatcgg attctggctt caacatttcg caggaatgca actaagagac attactgaat
      421 caaaaattta ttcagcaatg cagaaaatga cgaaccggcg tcatgaggaa aactggagac
      481 tcagggcaga a
//
LOCUS       DAJTUM010000334          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTUM010000334 DAJTUM010000000
VERSION     DAJTUM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..425)
                     /gene="mddA"
                     /locus_tag="OO471_004409"
     CDS             complement(<1..425)
                     /gene="mddA"
                     /locus_tag="OO471_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6245378.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQ"
BASE COUNT          134 a          138 c          108 g          110 t
ORIGIN      
        1 atttgtgcgg tgacgacaaa tcccagcgac tggtgcagat gcagcgaagc ctgattttgc
       61 gattcgatcc cggcgaccat gacatgcttc ccgcaatccc gcgcttcatc aatcaatcga
      121 cttaacaatt tacgccccag acctttgccc tgatgatcgg gatggacata aaccgaatgt
      181 tccacggtat ggcgaaaacc atcgaaacta cgccagtcgc caaacgaggc atatcccgta
      241 actacgccat cttcttcact taccagcacc ggataacctg ctatggtccg cgcttcaaac
      301 caggcaatgc ggttatctgc atccaccgtt tggtcattcc agatagccgc cgtatacaac
      361 acggcgtggt tataaatttc cgcaatggca gcacagtcgg ctttgcgggc aaaacggatg
      421 gacatgtctg gcctcaataa aataatgatg atgatgttat caatactatc gaaaacagcc
      481 cgaagcaacg
//
LOCUS       DAJTUM010000335          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTUM010000335 DAJTUM010000000
VERSION     DAJTUM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>484
                     /locus_tag="OO471_004410"
     CDS             <1..>484
                     /locus_tag="OO471_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6245379.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DAJTUM010000336          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTUM010000336 DAJTUM010000000
VERSION     DAJTUM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>484
                     /locus_tag="OO471_004411"
     CDS             <1..>484
                     /locus_tag="OO471_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245380.1"
                     /translation="ISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNS
                     QAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETG
                     QVWRTARILKDRSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFAND
                     G"
BASE COUNT          146 a          111 c          119 g          108 t
ORIGIN      
        1 catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa accgtaaacg
       61 aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct ttaccgatcg
      121 atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg agtatgactt
      181 tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa tgaaagctga
      241 aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa tgaacataga
      301 aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc gtattgatgg
      361 acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt ctcttaacct
      421 gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat ttgccaacga
      481 cggt
//
LOCUS       DAJTUM010000337          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTUM010000337 DAJTUM010000000
VERSION     DAJTUM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..412
                     /gene="istB"
                     /locus_tag="OO471_004412"
     CDS             <1..412
                     /gene="istB"
                     /locus_tag="OO471_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR6245381.1"
                     /translation="FLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTR
                     LNNSDLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTH
                     ADAILDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          139 a          104 c          123 g          110 t
ORIGIN      
        1 gttcctggca tgtgcacttg gtcataatgc ctgccgacag ggatacaagg tctactatta
       61 tcgccttaaa gcgctgatgg aacagtgcta tcaggggcat gctgatggaa gatacagcaa
      121 acttttgacc aggctgaata atagcgatct gctgcttctg gatgactggg ggctggaacc
      181 tctctcatca gaacagcgta gcgacctgct ggaaatagtg gatctgatgt accaacgagg
      241 ctcaatcatc gtagtgagcc agttgccggt ggaaaactgg tacaaaatga tcggagactc
      301 cacacatgcg gatgccatcc tagatcgact ggttcatggc agtatcaaga tcgaacttaa
      361 aggagaatca atgcggaaaa tacaatctcc gttgaccgaa ggagatcagt gaaggtaatt
      421 taaaaacggt tctgtgaaag tgacacgaac cgatctccat cgatgttact caccga
//
LOCUS       DAJTUM010000338          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTUM010000338 DAJTUM010000000
VERSION     DAJTUM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>472
                     /locus_tag="OO471_004413"
     CDS             <1..>472
                     /locus_tag="OO471_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6245382.1"
                     /translation="KAHIFLITSFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLR
                     WSEVNEMLQSGLVEFHVHTHTHTRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCS
                     KHLCWPEGYYNKDYIQIAEELGFHYLYTTERRMNAPAKGTTRIGRISTKERENCV"
BASE COUNT          162 a           78 c          104 g          128 t
ORIGIN      
        1 tcaaggcaca tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc
       61 ctggaaagga atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata
      121 atgttatgct tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc
      181 atgttcatac acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt
      241 gtaaacatct tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa
      301 aatgcagtaa acatttgtgt tggccggaag gttattataa caaggattat attcagatcg
      361 ctgaagaact tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta
      421 aaggaactac acggatagga agaataagca ccaaggaacg ggaaaattgc gt
//
LOCUS       DAJTUM010000339          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTUM010000339 DAJTUM010000000
VERSION     DAJTUM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>472
                     /locus_tag="OO471_004414"
     CDS             <1..>472
                     /locus_tag="OO471_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6245383.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTUM010000340          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTUM010000340 DAJTUM010000000
VERSION     DAJTUM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(110..>466)
                     /locus_tag="OO471_004415"
     CDS             complement(110..>466)
                     /locus_tag="OO471_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6245384.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          115 a          118 c           83 g          150 t
ORIGIN      
        1 cctgtgccat ttttcaggca taaaaaaacc gctcggcggc gggttttgct tactttacca
       61 tcgcgtacaa aatcggcaaa gtatcagatt tgcatgagat atatgccttt taatctactt
      121 ttgcaatact ttgctttgaa aatgccgctt tttgttctga acgtgttctc atcacaaaca
      181 ataaagcctc actatccagt cggtgaaaaa tgtgtttcat tacaacccag tgaccagtaa
      241 atgttttgga ccagtttttg gttgttactc ccgccagtaa tgccagctcc tgatattcat
      301 aaccttcccc accaaaaagt tctgctttta ccgcctgcgc cgccagccag attaattttt
      361 tcaggcgttc ctgtgttttc tctgcaattt tcctagtgcc agaatgagcc ttaaactctt
      421 cccacgccca gtgggttatg gtgatctgat actcccagca aatact
//
LOCUS       DAJTUM010000341          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTUM010000341 DAJTUM010000000
VERSION     DAJTUM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>463
                     /locus_tag="OO471_004416"
     CDS             <1..>463
                     /locus_tag="OO471_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6245385.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTUM010000342          449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTUM010000342 DAJTUM010000000
VERSION     DAJTUM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(160..>449)
                     /gene="pflA"
                     /locus_tag="OO471_004417"
     CDS             complement(160..>449)
                     /gene="pflA"
                     /locus_tag="OO471_004417"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="activates pyruvate formate-lyase 1 under anaerobic
                     conditions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6245386.1"
                     /translation="EFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVE
                     KIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT          120 a          105 c          100 g          124 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactcca
//
LOCUS       DAJTUM010000343          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTUM010000343 DAJTUM010000000
VERSION     DAJTUM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..373)
                     /locus_tag="OO471_004418"
     CDS             complement(<1..373)
                     /locus_tag="OO471_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245387.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           96 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcgg
//
LOCUS       DAJTUM010000344          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTUM010000344 DAJTUM010000000
VERSION     DAJTUM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..245
                     /locus_tag="OO471_004419"
     CDS             <1..245
                     /locus_tag="OO471_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6245388.1"
                     /translation="IVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTH
                     AGLLIDDEQFDETNIVRGLPVPGGRLGIKITELECA"
     gene            242..>440
                     /locus_tag="OO471_004420"
     CDS             242..>440
                     /locus_tag="OO471_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6245389.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIP"
BASE COUNT          121 a           96 c          126 g           97 t
ORIGIN      
        1 ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg ataaagatta
       61 ttgcagagcc accggataag cggcgtcgtg acctggacaa tatcctgaaa gcaccactgg
      121 atgcactgac acatgcgggg ttgcttatcg acgacgagca gtttgatgaa accaatattg
      181 tgcgcggact gcctgttcct ggtggtcggt tggggataaa aatcacagag ctggagtgtg
      241 catgaataac cagtatttac agtttgttcg tgagcagctc attatcgcca ccgctgattt
      301 gagtggggcg acaaaaggtc agcttgaagc ctggcaggag aatgccatgt tcgatacagg
      361 gcgttacagg cgtaaaaaaa tccggtaccg cgatgaggtg accggaaaaa tgataacgcg
      421 ggataatcca ccaatcccgg
//
LOCUS       DAJTUM010000345          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTUM010000345 DAJTUM010000000
VERSION     DAJTUM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            35..434
                     /locus_tag="OO471_004421"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO471_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTUM010000346          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTUM010000346 DAJTUM010000000
VERSION     DAJTUM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            21..179
                     /locus_tag="OO471_004422"
     CDS             21..179
                     /locus_tag="OO471_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6245390.1"
                     /translation="MASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRA
                     AFPRFKKR"
     gene            261..>428
                     /locus_tag="OO471_004423"
     CDS             261..>428
                     /locus_tag="OO471_004423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6245391.1"
                     /translation="MRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPAHPSAS
                     MVGLDAGVAKLA"
BASE COUNT          111 a          110 c          110 g           97 t
ORIGIN      
        1 aatacatccc ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa
       61 cgcaatggct taaagattct ccctcacagc cattgcaaca gtcactgaaa gaccttgagc
      121 gggcttacaa aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggtgac
      181 agaatgatgc attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt
      241 ttctgccgaa actgggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga
      301 aaaatgtcac tgtcagccag tcctgcggta cgtggtacat cagtattcag acagaaagtg
      361 aagtatccac tccggctcac ccttcagcat caatggtcgg gctggatgct ggcgtggcta
      421 aactcgcc
//
LOCUS       DAJTUM010000347          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTUM010000347 DAJTUM010000000
VERSION     DAJTUM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>421
                     /locus_tag="OO471_004424"
     CDS             <1..>421
                     /locus_tag="OO471_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6245392.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMD"
BASE COUNT           95 a          136 c          114 g           76 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 t
//
LOCUS       DAJTUM010000348          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTUM010000348 DAJTUM010000000
VERSION     DAJTUM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            78..143
                     /locus_tag="OO471_004425"
                     /pseudo
     CDS             78..143
                     /locus_tag="OO471_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          134 a           87 c           93 g          105 t
ORIGIN      
        1 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac tgggacctcg
       61 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
      121 ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga agatcgtcca
      181 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
      241 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
      301 ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
      361 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaa
//
LOCUS       DAJTUM010000349          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTUM010000349 DAJTUM010000000
VERSION     DAJTUM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            229..336
                     /locus_tag="OO471_004426"
     CDS             229..336
                     /locus_tag="OO471_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6245393.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           98 a          103 c          127 g           87 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg ccttt
//
LOCUS       DAJTUM010000350          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTUM010000350 DAJTUM010000000
VERSION     DAJTUM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            22..>415
                     /locus_tag="OO471_004427"
     CDS             22..>415
                     /locus_tag="OO471_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6245394.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDA"
BASE COUNT          132 a           88 c           87 g          108 t
ORIGIN      
        1 ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct cgttgttgca
       61 tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa gaaattcagc
      121 gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga caaggtgctt
      181 aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac caaagaagat
      241 gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg tgttgaagaa
      301 aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat ggaaaaagtg
      361 gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga tgcca
//
LOCUS       DAJTUM010000351          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTUM010000351 DAJTUM010000000
VERSION     DAJTUM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            155..340
                     /locus_tag="OO471_004428"
                     /pseudo
     CDS             155..340
                     /locus_tag="OO471_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAJTUM010000352          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTUM010000352 DAJTUM010000000
VERSION     DAJTUM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..347
                     /gene="rlmB"
                     /locus_tag="OO471_004429"
     CDS             <1..347
                     /gene="rlmB"
                     /locus_tag="OO471_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6245395.1"
                     /translation="RSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQEENIWIVG
                     TAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVAT
                     GICLFEAVRQRS"
BASE COUNT           91 a           93 c          115 g           90 t
ORIGIN      
        1 atcgctccgc acagctcaac gctacggcga aaaaagtagc ctgcggcgcg gcagaaagcg
       61 ttccgctgat tcgggtgact aaccttgcgc gcaccatgcg tatgttgcag gaagagaata
      121 tctggatcgt cggtacggca ggcgaggcgg atcatacact ctatcagagc aaaatgaccg
      181 gacgcctggc gctggtgatg ggcgcggaag gtgaaggtat gcgtcgcctg acccgtgaac
      241 attgcgatga gttgatcagc atcccaatgg caggaagtgt ttcttccctg aacgtttcgg
      301 ttgcaaccgg aatttgctta tttgaagcgg ttcgtcagcg cagctagttc ctctgaatta
      361 tgaaaagcca tccagatttg gatggtttt
//
LOCUS       DAJTUM010000353          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTUM010000353 DAJTUM010000000
VERSION     DAJTUM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            260..318
                     /locus_tag="OO471_004430"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO471_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTUM010000354          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTUM010000354 DAJTUM010000000
VERSION     DAJTUM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(11..>371)
                     /locus_tag="OO471_004431"
     CDS             complement(11..>371)
                     /locus_tag="OO471_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6245396.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT           69 a          129 c           82 g           91 t
ORIGIN      
        1 ttagcctgtg tcaggccggc atatacctca gcagactgat atcccgcgcg tttttcttca
       61 gcccctccac attcagtccc gcgatggccc ggcggtatac catccggccg ccacacagca
      121 cgcattcgaa cgggtcacga ctcaggaact gtttcaccat ttgtgcatag cacactttcg
      181 ccactggttc cggtttatcc atccccagag cacggtacac ctgcggcagc ttctctccac
      241 acacccggtt ggcaaggaac ccgaagtacc tcaccatctt aaaaaacttc tccgggatgt
      301 gctgtttcag cctcgcgacc agctcacgct gtgtcagcgt ttccgtcgcc gtttctcccg
      361 ttttgtggtc c
//
LOCUS       DAJTUM010000355          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTUM010000355 DAJTUM010000000
VERSION     DAJTUM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAJTUM010000356          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTUM010000356 DAJTUM010000000
VERSION     DAJTUM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            39..>357
                     /locus_tag="OO471_004432"
     CDS             39..>357
                     /locus_tag="OO471_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6245397.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAJTUM010000357          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTUM010000357 DAJTUM010000000
VERSION     DAJTUM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>356
                     /locus_tag="OO471_004433"
     CDS             <1..>356
                     /locus_tag="OO471_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6245398.1"
                     /translation="GEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFL
                     IAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDI
                     DAALTGTLKTANHLSE"
BASE COUNT           91 a           88 c          112 g           65 t
ORIGIN      
        1 tggtgaatac tgtttccatg cgcggactgg aaaatatggt gtgtatctga aacaggactg
       61 gcgcaacgag tacaacgttg gcgacattgc tgtatatgag gactcaaagc ccggcacgct
      121 gaatgacttt ctgattgctc ctgatgaggg cgacctgaaa ccggatgtcg tcaaacgctt
      181 tgaggaaatg gtggcgcagg cgcagcagag cgccggggcc gcagccggaa acgcacagca
      241 gacggcgcag gatgtggcgg cagccgcagg ttatgcccgc gcagcagagc aggccaaaaa
      301 tgacattgat gctgcgctga ccggcactct gaaaacggct aaccatctgt ctgaaa
//
LOCUS       DAJTUM010000358          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTUM010000358 DAJTUM010000000
VERSION     DAJTUM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..306
                     /locus_tag="OO471_004434"
     CDS             <1..306
                     /locus_tag="OO471_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6245399.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
BASE COUNT           97 a           79 c           83 g           93 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg ta
//
LOCUS       DAJTUM010000359          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTUM010000359 DAJTUM010000000
VERSION     DAJTUM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO471_004435"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO471_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6245400.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTUM010000360          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTUM010000360 DAJTUM010000000
VERSION     DAJTUM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(166..>323)
                     /locus_tag="OO471_004436"
     CDS             complement(166..>323)
                     /locus_tag="OO471_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6245401.1"
                     /translation="WRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPL
                     ARMRWCG"
BASE COUNT           80 a          105 c           66 g           72 t
ORIGIN      
        1 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
       61 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      121 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      181 catccgggcc agcggctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      241 ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc agcgggtcaa ccttcagtaa
      301 cctctgatac atccctctcc agg
//
LOCUS       DAJTUM010000361          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTUM010000361 DAJTUM010000000
VERSION     DAJTUM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..206)
                     /locus_tag="OO471_004437"
     CDS             complement(<1..206)
                     /locus_tag="OO471_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245402.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSL"
     gene            complement(196..>322)
                     /locus_tag="OO471_004438"
     CDS             complement(196..>322)
                     /locus_tag="OO471_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCR6245403.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT           99 a           89 c           73 g           61 t
ORIGIN      
        1 tccagactcc agtaaggaac aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg
       61 aatacctgac tggggatatc atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga
      121 gatccatagg tgaagaagaa aaacggggcc agcaacaata accagccagc gccttgtagc
      181 aatacgttcc gacgttcagt tatcacgacg caccgccatc gaaaccgtaa aaatgcccca
      241 cacatcaatc cgttgagtgc atttatcaaa tccggcatta cgcacgagcg aatccatctc
      301 cccttgcgaa cgcacgcgca tt
//
LOCUS       DAJTUM010000362         5818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.guided.171, whole genome shotgun sequence.
ACCESSION   DAJTUM010000362 DAJTUM010000000
VERSION     DAJTUM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.guided.171"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO471_004439"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO471_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6245404.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="OO471_004440"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="OO471_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /locus_tag="OO471_004441"
     CDS             2144..2617
                     /locus_tag="OO471_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6245405.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="OO471_004442"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="OO471_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="OO471_004443"
     CDS             2967..3803
                     /locus_tag="OO471_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6245406.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="OO471_004444"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="OO471_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="OO471_004445"
     CDS             complement(4358..4747)
                     /locus_tag="OO471_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6245407.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="OO471_004446"
     CDS             4965..5405
                     /locus_tag="OO471_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6245408.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1399 a         1539 c         1517 g         1363 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggataaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcattcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgta
//
LOCUS       DAJTUM010000363         5095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.guided.190, whole genome shotgun sequence.
ACCESSION   DAJTUM010000363 DAJTUM010000000
VERSION     DAJTUM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.guided.190"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..776)
                     /locus_tag="OO471_004447"
     CDS             complement(<1..776)
                     /locus_tag="OO471_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR6245409.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGG"
     gene            complement(779..1339)
                     /locus_tag="OO471_004448"
     CDS             complement(779..1339)
                     /locus_tag="OO471_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6245410.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1465..1815)
                     /locus_tag="OO471_004449"
     CDS             complement(1465..1815)
                     /locus_tag="OO471_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6245411.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTSEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(2018..3031)
                     /gene="intI1"
                     /locus_tag="OO471_004450"
     CDS             complement(2018..3031)
                     /gene="intI1"
                     /locus_tag="OO471_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6245412.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3241..4071
                     /locus_tag="OO471_004451"
     CDS             3241..4071
                     /locus_tag="OO471_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6245413.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            4184..4975
                     /gene="aadA1"
                     /locus_tag="OO471_004452"
     CDS             4184..4975
                     /gene="aadA1"
                     /locus_tag="OO471_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6245414.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1231 a         1378 c         1388 g         1098 t
ORIGIN      
        1 ccgccctcgc gggcaatctt gagcaggcgg ttctggtgaa ccagccgctc gatgccggta
       61 ggcagatcga gtgcctgcca tgccttgagg cgttcgatgt gttccagcat atgccgcgaa
      121 tttggcttgg ccggagactg gcgcaaccaa gccaaccagg tcgtcttgcc gttgtcccgg
      181 cgcttgagca gatcgtcgag gcggcggcga tgcgcgtccg ccagtggttc ggccaaggcg
      241 tcgtagatgc gccggttagc acgggtgatc gcctcggcac tcgcccgctc gacggcgttg
      301 agggcgggca gaatgaccga ctgccgccgc aggtgcccga tcaaggcgct ggccagcacg
      361 atgcctttgt cggtttgcat cgccagctcg gtcagcatct ggacggcctg ccggtaatgg
      421 ctcatggtga agggccggaa accgaacacg gtttgcagct cgctcaggtg ctcgcgccgg
      481 gtctgctccc gctggccgta ctcgttccag ctttcgacgc cgaccttgag ctggtcggcg
      541 accagcttca acaagggcgg gaacggtagt tcatcgacgc ccaggatgac gccgggaaag
      601 cgcaggtaac agagctgcac cgcgaagccc agccgattgg ctggcccgcg ccgctgtcgg
      661 atgatcgaga ggtcggtatc gttgaatgtg taatgtcgga tcaggtcgtc cttggagtcc
      721 ggcaacgcca gcaggctttc ccgctcggcg gcggacagga tggaacgacg tggcatattt
      781 actgatccgt tctcaagtat tgatacaggg tttcgcgact gattccgaat tcacgagcaa
      841 gcttggtctt ttgctcgcca gcctcgacac gttggcgcag ttcggcaata cgctcagacg
      901 acagggattt cttcctgcca cggtaagccc cgcgttgctt ggcgagcgca ataccctcgc
      961 gctgacgctc gcggatcagg gcgcgctcga actcggcgaa cgcgcccatc accgagagca
     1021 tcaggttcgc catcggagag tcttcgccag taaaactgag gtgttccttg acgaattcga
     1081 tatgcacgcc gcgttgtgtc agcgtttgca cgatccggcg caaatcatcg agattgcgcg
     1141 ccaggcgatc catgctatgc accaccacgg tgtcgccggt gcgggcgaag cttatcagcg
     1201 cttccagttg cggacgcttg acatccttgc cggatgcctt gtcgctaaaa gcgcgatcaa
     1261 ccttgacgcc ttccagttgc cgttccgggt tctggtcgaa ggtgctgacc ctgatatacc
     1321 caatgcgctg tccagtcatg gaattccctg caaaatgtca gggaagactc tatgaccttc
     1381 aacgagatat gtcaataaat tcaaaattca atcctatcct gacgcaattt acacatggca
     1441 tctgacatca ggttagggta tgcctcaacc tgacggcggc gaatcacaag cgtccggttt
     1501 gacgctggtt tcggtcgcag tgctggcccg cgcctggaag cgctgatagc actccagccc
     1561 gcagaaatgc tccacgtact cggccccttc cgacgtgaag gcggcatcga gcgggatttc
     1621 cttgcagcac acgcagcaac tggtgctcgg ttcattggcg ttcatggtgg tgttcctcca
     1681 ttggttgacg aagccgacga aggccgccgc cggcatcggc ctggcgaaca ggaaaccctg
     1741 caccgtgtcg caacccgcct gccgcaacca cgcaaggcag tcgggtgttt ccacaccctc
     1801 ggctaccacc tccattgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
     1861 cactatagca gcggaggggt tggatccatc aggcaacgac gggctgctgc cggccatcag
     1921 cggacgcagg gaggactttc cgcaaccggc cgttcgatgc ggcaccgatg gccttcgcgc
     1981 aggggtagtg aatccgccag gattgacttg cgctgcccta cctctcacta gtgaggggcg
     2041 gcagcgcatc aagcggtgag cgcactccgg caccgccaac tttcagcaca tgcgtgtaaa
     2101 tcatcgtcgt agagacgtcg gaatggccga gcagatcctg cacggttcga atgtcgtaac
     2161 cgctgcggag caaggccgtc gcgaacgagt ggcggagggt gtgcggtgtg gcgggcttcg
     2221 tgatgcctgc ttgttctacg gcacgtttga aggcgcgctg aaaggtctgg tcatacatgt
     2281 gatggcgacg cacgacaccg ctccgtggat cggtcgaatg cgtgtgctgc gcaaaaaccc
     2341 agaaccacgg ccaggaatgc ccggcgcgcg gatacttccg ctcaagggcg tcgggaagcg
     2401 caacgccgct gcggccctcg gcctggtcct tcagccacca tgcccgtgca cgcgacagct
     2461 gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc ttggagccct
     2521 tgccctcccg cacgatgatc gtgccgtgat cgaaatccag atccttgacc cgcagttgca
     2581 aaccctcact gatccgcatg cccgttccat acagaagctg ggcgaacaaa cgatgctcgc
     2641 cttccagaaa accgaggatg cgaaccactt catccggggt cagcaccacc ggcaagcgcc
     2701 gcgacggccg aggtcttccg atctcctgaa gccagggcag atccgtgcac agcaccttgc
     2761 cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa accttgcgct
     2821 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac
     2881 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     2941 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     3001 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     3061 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     3121 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttgggcgaac
     3181 ccggagcctc attaattgtt agccgttaaa attaagccct ttaccaaacc aatacttatt
     3241 atgaaaaaca caatacatat caacttcgct atttttttaa taattgcaaa tattatctac
     3301 agcagcgcca gtgcatcaac agatatctct actgttgcat ctccattatt tgaaggaact
     3361 gaaggttgtt ttttacttta cgatgcatcc acaaacgctg aaattgctca attcaataaa
     3421 gcaaagtgtg caacgcaaat ggcaccagat tcaactttca agatcgcatt atcacttatg
     3481 gcatttgatg cggaaataat agatcagaaa accatattca aatgggataa aacccccaaa
     3541 ggaatggaga tctggaacag caatcataca ccaaagacgt ggatgcaatt ttctgttgtt
     3601 tgggtttcgc aagaaataac ccaaaaaatt ggattaaata aaatcaagaa ttatctcaaa
     3661 gattttgatt atggaaatca agacttctct ggagataaag aaagaaacaa cggattaaca
     3721 gaagcatggc tcgaaagtag cttaaaaatt tcaccagaag aacaaattca attcctgcgt
     3781 aaaattatta atcacaatct cccagttaaa aactcagcca tagaaaacac catagagaac
     3841 atgtatctac aagatctgga taatagtaca aaactgtatg ggaaaactgg tgcaggattc
     3901 acagcaaata gaaccttaca aaacggatgg tttgaagggt ttattataag caaatcagga
     3961 cataaatatg tttttgtgtc cgcacttaca ggaaacttgg ggtcgaattt aacatcaagc
     4021 ataaaagcca agaaaaatgc gatcaccatt ctaaacacac taaatttata aaaaatctaa
     4081 tggcaaaatc gcccaaccct tcaatcaagt cgggacggcc aaaagcaagc ttttggctcc
     4141 cctcgctggc gctcggcgcc ccttatttca aacgttaaac atcatgaggg aagtggtgat
     4201 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     4261 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     4321 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     4381 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     4441 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     4501 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     4561 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     4621 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     4681 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     4741 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     4801 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     4861 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     4921 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     4981 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     5041 taagcacata attgcggtga tgctgccaac ttactgattt agtgtatgat ggtgt
//
LOCUS       DAJTUM010000364         4987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.guided.193, whole genome shotgun sequence.
ACCESSION   DAJTUM010000364 DAJTUM010000000
VERSION     DAJTUM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.guided.193"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            <1..258
                     /locus_tag="OO471_004453"
     CDS             <1..258
                     /locus_tag="OO471_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HCR6245415.1"
                     /translation="LFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFEL
                     APKAANIIVKDEKMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            269..628
                     /gene="frdD"
                     /locus_tag="OO471_004454"
     CDS             269..628
                     /gene="frdD"
                     /locus_tag="OO471_004454"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6245416.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            691..1824
                     /locus_tag="OO471_004455"
     CDS             691..1824
                     /locus_tag="OO471_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6245417.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1913..2446
                     /gene="blc"
                     /locus_tag="OO471_004456"
     CDS             1913..2446
                     /gene="blc"
                     /locus_tag="OO471_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6245418.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2443..2760)
                     /gene="sugE"
                     /locus_tag="OO471_004457"
     CDS             complement(2443..2760)
                     /gene="sugE"
                     /locus_tag="OO471_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6245419.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2936..3082)
                     /gene="ecnB"
                     /locus_tag="OO471_004458"
     CDS             complement(2936..3082)
                     /gene="ecnB"
                     /locus_tag="OO471_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6245420.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3193..3318)
                     /gene="ecnA"
                     /locus_tag="OO471_004459"
     CDS             complement(3193..3318)
                     /gene="ecnA"
                     /locus_tag="OO471_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6245421.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3370..3936)
                     /gene="efp"
                     /locus_tag="OO471_004460"
     CDS             complement(3370..3936)
                     /gene="efp"
                     /locus_tag="OO471_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6245422.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3978..>4987
                     /gene="epmB"
                     /locus_tag="OO471_004461"
     CDS             3978..>4987
                     /gene="epmB"
                     /locus_tag="OO471_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6245423.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDL"
BASE COUNT         1234 a         1276 c         1274 g         1203 t
ORIGIN      
        1 ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt tttacaaaac
       61 ccggttatcg tgatcattaa cctgatcact ctgacggcag ctctgctgca caccaaaacc
      121 tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca
      181 gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt
      241 gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga
      301 accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt
      361 gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg atgcgctgag
      421 ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat
      481 gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa
      541 aatccacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt
      601 cacgctgatt ggtgtcgtta caatctaacg catcgccaat gtaaatccgg cccgcctatg
      661 gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg cgccttatta
      721 attaccgcct cttactccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc
      781 acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat
      841 cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
      901 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt
      961 ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa atactggcct
     1021 gaacttaccg ctaaacagtg gaatgggatc acactattac atcttgcgac ctacaccgct
     1081 ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt gctgcgcttc
     1141 tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc caactccagt
     1201 atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg
     1261 caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt acctccccca
     1321 gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgccaggg
     1381 gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg
     1441 caaagcaatt taccccccct tgatatcaat gagaaaacgc ttcaacaagg gatacaactg
     1501 gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg
     1561 gactggccgg tcaatcctga cagcatcatt aacggcagtg acaataaaat tgcactggca
     1621 gcacgcccgg taaaagcgat taagccccca actcctgcag tacgcgcatc atgggtacat
     1681 aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
     1741 ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgt
     1801 cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc
     1861 gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct
     1921 gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgta gttttcctac
     1981 gccgccgcgt ggcgtgaccg tagtgaataa tttcgacgcc aaacgttatc tcggtacctg
     2041 gtatgagatt gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag tcaccgcaac
     2101 atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata accctgacag
     2161 aggaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa ctcgcgctgc
     2221 gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg cactcgatcg
     2281 ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga tactctcccg
     2341 cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga cccgggaagg
     2401 gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag tgctgagttt
     2461 cagaccaata atccccaata cgattaacgc cagactcgcc agacgcatcg ggttagcgga
     2521 ctcaccgagc agcacaatgc cggtgatggc cgcgccgact gcgccaatac ccgtccacac
     2581 ggcataagcc gtccctaccg gtaacgattt catcgcccag gcaagtagcg ccatactgac
     2641 tatcatcgcc gtcacggtaa taacactcgg cgtcaaacgg ctaaagccgt gggtatattt
     2701 caggccaacg gcccatacca cttccagcag accagcaata actaagataa tccaggacat
     2761 atcaggctcc ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg
     2821 caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt
     2881 tatgacgatc gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt
     2941 gctgcgcttt cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa
     3001 cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg
     3061 ctgcaattgt cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct
     3121 atagcaaatt aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga
     3181 agagaagaaa aattagctgg cagcgcgaga gatggagtta ccgagatgtt tgatgtcttc
     3241 gccgaaaccg caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac
     3301 gataaggcgt ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc
     3361 acaaccgcat tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac
     3421 ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg
     3481 tttgccgcca gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag
     3541 ttcaacgaag ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca
     3601 ctctgcctga tccagcagcc atttagcatt gtcgccaatt gcttttgcat cagcagacag
     3661 ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt
     3721 caggttcata tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc
     3781 tacgcgagta ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc
     3841 cggttttacg aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag
     3901 accagcacga aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt
     3961 ggtagctaag ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt
     4021 ggttaacgca acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata
     4081 tagacgcgga cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg
     4141 tgccccgctc atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc
     4201 aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg
     4261 aagaacagca cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc
     4321 tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg
     4381 ccgaaaatca gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc
     4441 cggaactgga cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gagcacgagc
     4501 tggactggtt gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca
     4561 gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc
     4621 gttctacgct gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa
     4681 cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg
     4741 ttctgttacg tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt
     4801 tcgatgccgg cgtgatgccg tattacctgc atgtgctaga taaagtacag ggcgcggcgc
     4861 attttatggt gagtgatgac gaagcacggc agattatgcg tgagttgctg acattggtgt
     4921 cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc
     4981 tggatct
//
LOCUS       DAJTUM010000365         3974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.guided.212, whole genome shotgun sequence.
ACCESSION   DAJTUM010000365 DAJTUM010000000
VERSION     DAJTUM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.guided.212"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            complement(<1..268)
                     /gene="tetR(B)"
                     /locus_tag="OO471_004462"
     CDS             complement(<1..268)
                     /gene="tetR(B)"
                     /locus_tag="OO471_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6245424.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA"
     gene            350..1555
                     /gene="tet(B)"
                     /locus_tag="OO471_004463"
     CDS             350..1555
                     /gene="tet(B)"
                     /locus_tag="OO471_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6245425.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1668..2261)
                     /gene="tetC"
                     /locus_tag="OO471_004464"
     CDS             complement(1668..2261)
                     /gene="tetC"
                     /locus_tag="OO471_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6245426.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2394..2765
                     /locus_tag="OO471_004465"
     CDS             2394..2765
                     /locus_tag="OO471_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6245427.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(2775..>3974)
                     /locus_tag="OO471_004466"
     CDS             complement(2775..>3974)
                     /locus_tag="OO471_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6245428.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1055 a          747 c          859 g         1313 t
ORIGIN      
        1 aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta
       61 aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag
      121 cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga
      181 gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa
      241 tcactttact tttatctaat ctagacatca ttaattccta atttttgttg acactctatc
      301 attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa tgaatagttc
      361 gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc ttatcatgcc
      421 agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta accactttgg
      481 cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc ttggaaaaat
      541 gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg catcgctgga
      601 ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc gtttgctttc
      661 agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata ccacctcagc
      721 ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg gtttaatagc
      781 ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct tttttatcgc
      841 tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg aaaccaaaaa
      901 tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt cggtatacat
      961 cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc aattgatagg
     1021 ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat ggaatagcat
     1081 gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc aagcctttgt
     1141 ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg gatttattgc
     1201 agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag ttttccctgt
     1261 tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag tgatgtctat
     1321 ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc ttaccaatgc
     1381 aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac taccaatttg
     1441 ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc tgctatcgat
     1501 gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg cttagttatt
     1561 tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac ccaagagggc
     1621 attttttacg ataaagaaga tttagcttca aataaaacct atctatttta tttatctttc
     1681 aagctcaata aaaagccgcg gtaaatagca ataaattggc cttttttatc ggcaagctct
     1741 tttaggtttt tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa gtttttaagc
     1801 acatcatcat cataagcttt aagttggttc tcttggatca atttgctgac aatggcgttt
     1861 accttaccag taatgtattc aaggctaatt ttttcaagtt cattccaacc aatgataggc
     1921 atcacttctt ggatagggat aaggttttta ttattatcaa taatataatc aagataatgt
     1981 tcaaatatac tttctaaggc agaccaacca tttgttaaat cagtttttgt tgtgatgtag
     2041 gcatcaatca taattaattg ctgcttataa caggcactga gtaattgttt tttattttta
     2101 aagtgatgat aaaaggcacc tttggtcacc aacgcttttc ccgagatctc atctattgaa
     2161 acagcttgat agcctttttc aacaaacaat attcgtgctg agttaaccag tgattgatag
     2221 gtactcttaa aattttcttg ttgatgattt ttattttcca tgatagattt aaaataacat
     2281 accgtcagta tgtttatggt atcatgatga tgtggtcgtg acaatcttaa gaacatttag
     2341 gttattttat gtatattgaa cagcattctc gctatcaaaa taaagctaat aacatccaat
     2401 taagatatga tgataagcag tttcatacaa cggttatcaa agatgttcta ttatggattg
     2461 aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg ggttatacca
     2521 agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct agctatattc
     2581 gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa actatccttg
     2641 agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcga tttaagtaca
     2701 tttttaaggt tacaccaagt tattatcggc gtaataaatt atgggaattg gaggcaatgc
     2761 actgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct
     2821 agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa
     2881 acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg
     2941 aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga
     3001 agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg
     3061 cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt
     3121 cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt
     3181 gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa
     3241 tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt
     3301 tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta
     3361 gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat
     3421 tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac
     3481 catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg
     3541 ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg
     3601 aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc
     3661 ataagtcgtt tttgctcacg aatatcagac cagtcaacaa gtacaatggg catcgtattg
     3721 cccgaacaga taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga
     3781 cgattaccta acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc
     3841 aggttacggc caagttcggt aagagtgaga gttttacagt caagtaatgc gtggcaagcc
     3901 aacgttaagc tgttgagtcg ttttaagtgt aattcggggt aatgactcca acttactgat
     3961 agtgttttat gttc
//
LOCUS       DAJTUM010000366         2159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.guided.248, whole genome shotgun sequence.
ACCESSION   DAJTUM010000366 DAJTUM010000000
VERSION     DAJTUM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.guided.248"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            264..923
                     /locus_tag="OO471_004467"
     CDS             264..923
                     /locus_tag="OO471_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6245429.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OO471_004468"
     CDS             complement(1124..1501)
                     /locus_tag="OO471_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6245430.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>2159)
                     /locus_tag="OO471_004469"
     CDS             complement(1568..>2159)
                     /locus_tag="OO471_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6245431.1"
                     /translation="YDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRK
                     LGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFF
                     NRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYL
                     SPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          525 a          541 c          562 g          531 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatag
//
LOCUS       DAJTUM010000367          561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 102017
            SAMEA8899808-rid15816143.guided.329, whole genome shotgun sequence.
ACCESSION   DAJTUM010000367 DAJTUM010000000
VERSION     DAJTUM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899808
            Sequence Read Archive: ERR6004881
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477795.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477795.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:50:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,391
            Genes (coding)                    :: 3,993
            CDSs (with protein)               :: 3,993
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 185 of 398
            Pseudo Genes (incomplete)         :: 110 of 398
            Pseudo Genes (internal stop)      :: 147 of 398
            Pseudo Genes (multiple problems)  :: 43 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899808-rid15816143.guided.329"
                     /serovar="S. flexneri 2a"
                     /isolate="102017"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2009-01-18"
     gene            106..>561
                     /gene="gpt"
                     /locus_tag="OO471_004470"
     CDS             106..>561
                     /gene="gpt"
                     /locus_tag="OO471_004470"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6245432.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          132 a          139 c          146 g          144 t
ORIGIN      
        1 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
       61 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
      121 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
      181 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggggcgtta
      241 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
      301 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
      361 gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca
      421 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
      481 gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc
      541 gtcccgccaa tctccggtcg c
//
